CSPI01G10980 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G10980
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	gibberellin 2-beta-dioxygenase 8
Location	Chr1: 6873410 .. 6875374 (+)
RNA-Seq Expression	CSPI01G10980
Synteny	CSPI01G10980

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAGAAAAAACAAGATCAACATAAAGAAAAGAAAAAGGGTCTTTGTTATTATTATTTGCTTCCTTCTTTTAATTAATTTGTCTTCTATTTCTCCGATTACTTTGTCTTCTTATACAATAAATTTGATAATCACCACTTTTAATTTTGTGTCATAATTTATATCAGTCGTCTGCAAACGTGGCCATGGACCCACCATTTCACGAGGCTTACAAGACCCTCTTGGCCGAAAGGGCGGCGGCGGCGACGGGTGCAAAGGGAGCCAGCGACGTCGTCCTCACGGTGGTCGAGGAATGGGATCTTCCGCTGGTTGATTTAGAGCGTTTGACGGCGGGCAAAGTGGAAGAGGTGGAACAATGCAAAAATGACATAATTACAGCTTCTAAGGAATGGGGTTTCTTTCAAGTTGTGAATCATGGGATTTCAAATCAGCTTTTGGCCAAAATGAGAGCTAAGCAAATCGAGCTTTTCAAGCAGCCGTTTGAGCGTAAGAGTAAAGAAGATCAATTTTCGAATTTCTCCGCCGGAAGTTACCGTTGGGGAACCCCCTCCGCCACTTCTATTACACAACTTTCTTGGTCCGAAGCTTTTCATGTTTCTCTATCTGATATTCTTGGAACCAACGGCTCCGATGATGATGATCTCAGGTACAATATCACACCTTTTTCTTCTTCTTTTCTCTTGATGAAAATTGTTTGAGCTTTAACGGAATAAAGAAACTCACCTCCACTTAAACAGTCTCAGTTTAGATTCACTTTTTTTTCTCGTTTTGATATATATTTTGTTTTTGTAATTATACTAACAAACACAAATTTTTACTCATGGTTTTCTTATTATATATTATGATATACCATAAAACGTTTTCAAAACTCATGCAATTTTTTTTTGAAAAACTGAAAAATATTACTTTTTAGATTGAAGAAATGAATTATCCCTCCTTACCTTATTTAATATGGTATTATAGTGTGTAAAATGTAAATAGATTATAACATCGAAAATCGGTTGAAGATGATCATCTCCATAAGAAACTTTTGACAAGTTTGATTTACTCCACAAGTTAAAAGAGATAAAAGAATAAGAATAATATATACATATATATATGTATGTACTTTGATATATATTCCTGATAATAGAAGTTGTACTAGAATAGATTAAATTCTTTGATACCATACATAGCATGTAGAGGAAGAGAGGCAAATATAATTAAAGAAAGGGTTTAATAATAAATGAAAAGGATGCATTCAGGTCAACAATGGAAGAATATGCGGGAAAAGTTTCAAGGTTAGCACAGAAGCTAGCAGAGATATTGGGAGAGAACTTGGGGAGAAGCTCAAAATTCTTCGTGGAAAATTGCGTGCCCAGCACGTGCTATCTCCGGATGAACCGGTACCCGCCATGCCATGTTCCGGGTCAAATATTCGGTCTGATGCCACACACCGACAGCGATTTTCTCACCATTCTTCACCAAGATCAAGTCGGCGGCTTGGAGTTGGTCAAAGATGGGAAATGGATCGCCGTTAAACCCAATCCCCAGGCTCTTATTATCAACATAGGAGACTTATTTCAGGTAATACTTAATTACGACTTTTTTGTTTACTCTTTTATGCTATTTCTGTTCACAATTAATTAATTGATATTTGTACTGCAGGTTTGGAGTAACGATGAATACAAAAGTGTAGAGCATAGAGTTGTGACAAACTCGAAAAAGGAGAGATATTCAATAGCCTATTTTTTGTGTCCATGGAGTGAGACGGTGATAAAGAGCAAGAGTGAGCCAGGGGTTTATAGAAGGTTTAGTTTTAGGGAATTCAGGAATCAAGTTCAAGAAGATGTTCGTAAGTATGGCTACAAGATTGGTTTGCCTAGGTTTGTCTTATGATCCATCAAACTTCAAACCCCAATTTTTTCAAAGAAAATAATCTCGCTCATCTATATATATATATATACAAGTTTGTTTTTTTGGATTT

mRNA sequence

AAAAGAAAAAACAAGATCAACATAAAGAAAAGAAAAAGGTCGTCTGCAAACGTGGCCATGGACCCACCATTTCACGAGGCTTACAAGACCCTCTTGGCCGAAAGGGCGGCGGCGGCGACGGGTGCAAAGGGAGCCAGCGACGTCGTCCTCACGGTGGTCGAGGAATGGGATCTTCCGCTGGTTGATTTAGAGCGTTTGACGGCGGGCAAAGTGGAAGAGGTGGAACAATGCAAAAATGACATAATTACAGCTTCTAAGGAATGGGGTTTCTTTCAAGTTGTGAATCATGGGATTTCAAATCAGCTTTTGGCCAAAATGAGAGCTAAGCAAATCGAGCTTTTCAAGCAGCCGTTTGAGCGTAAGAGTAAAGAAGATCAATTTTCGAATTTCTCCGCCGGAAGTTACCGTTGGGGAACCCCCTCCGCCACTTCTATTACACAACTTTCTTGGTCCGAAGCTTTTCATGTTTCTCTATCTGATATTCTTGGAACCAACGGCTCCGATGATGATGATCTCAGGTCAACAATGGAAGAATATGCGGGAAAAGTTTCAAGGTTAGCACAGAAGCTAGCAGAGATATTGGGAGAGAACTTGGGGAGAAGCTCAAAATTCTTCGTGGAAAATTGCGTGCCCAGCACGTGCTATCTCCGGATGAACCGGTACCCGCCATGCCATGTTCCGGGTCAAATATTCGGTCTGATGCCACACACCGACAGCGATTTTCTCACCATTCTTCACCAAGATCAAGTCGGCGGCTTGGAGTTGGTCAAAGATGGGAAATGGATCGCCGTTAAACCCAATCCCCAGGCTCTTATTATCAACATAGGAGACTTATTTCAGGTTTGGAGTAACGATGAATACAAAAGTGTAGAGCATAGAGTTGTGACAAACTCGAAAAAGGAGAGATATTCAATAGCCTATTTTTTGTGTCCATGGAGTGAGACGGTGATAAAGAGCAAGAGTGAGCCAGGGGTTTATAGAAGGTTTAGTTTTAGGGAATTCAGGAATCAAGTTCAAGAAGATGTTCGTAAGTATGGCTACAAGATTGGTTTGCCTAGGTTTGTCTTATGATCCATCAAACTTCAAACCCCAATTTTTTCAAAGAAAATAATCTCGCTCATCTATATATATATATATACAAGTTTGTTTTTTTGGATTT

Coding sequence (CDS)

ATGGACCCACCATTTCACGAGGCTTACAAGACCCTCTTGGCCGAAAGGGCGGCGGCGGCGACGGGTGCAAAGGGAGCCAGCGACGTCGTCCTCACGGTGGTCGAGGAATGGGATCTTCCGCTGGTTGATTTAGAGCGTTTGACGGCGGGCAAAGTGGAAGAGGTGGAACAATGCAAAAATGACATAATTACAGCTTCTAAGGAATGGGGTTTCTTTCAAGTTGTGAATCATGGGATTTCAAATCAGCTTTTGGCCAAAATGAGAGCTAAGCAAATCGAGCTTTTCAAGCAGCCGTTTGAGCGTAAGAGTAAAGAAGATCAATTTTCGAATTTCTCCGCCGGAAGTTACCGTTGGGGAACCCCCTCCGCCACTTCTATTACACAACTTTCTTGGTCCGAAGCTTTTCATGTTTCTCTATCTGATATTCTTGGAACCAACGGCTCCGATGATGATGATCTCAGGTCAACAATGGAAGAATATGCGGGAAAAGTTTCAAGGTTAGCACAGAAGCTAGCAGAGATATTGGGAGAGAACTTGGGGAGAAGCTCAAAATTCTTCGTGGAAAATTGCGTGCCCAGCACGTGCTATCTCCGGATGAACCGGTACCCGCCATGCCATGTTCCGGGTCAAATATTCGGTCTGATGCCACACACCGACAGCGATTTTCTCACCATTCTTCACCAAGATCAAGTCGGCGGCTTGGAGTTGGTCAAAGATGGGAAATGGATCGCCGTTAAACCCAATCCCCAGGCTCTTATTATCAACATAGGAGACTTATTTCAGGTTTGGAGTAACGATGAATACAAAAGTGTAGAGCATAGAGTTGTGACAAACTCGAAAAAGGAGAGATATTCAATAGCCTATTTTTTGTGTCCATGGAGTGAGACGGTGATAAAGAGCAAGAGTGAGCCAGGGGTTTATAGAAGGTTTAGTTTTAGGGAATTCAGGAATCAAGTTCAAGAAGATGTTCGTAAGTATGGCTACAAGATTGGTTTGCCTAGGTTTGTCTTATGA

Protein sequence

MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGTPSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLGRSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL*

Homology

[Close]

BLAST of CSPI01G10980 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 423.3 bits (1087), Expect = 2.5e-117
Identity = 207/339 (61.06%), Postives = 255/339 (75.22%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGA---SDVVLTVVEEWDLPLVDLERLTAGKVEEVEQ 60
           MDPPF+E Y  LL  +             S  V  VVEE +LP++D+ RL  G  EE E+
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDNDVSEIPFSFSVTAVVEEVELPVIDVSRLIDGAEEEREK 60

Query: 61  CKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYR 120
           CK  I  AS+EWGFFQV+NHGIS  +L KMR +QI +F++PF++KSK ++   FSAGSYR
Sbjct: 61  CKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEK---FSAGSYR 120

Query: 121 WGTPSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGE 180
           WGTPSATSI QLSWSEAFHV ++DI  ++  D   L STME++A +   LA  LAE+L E
Sbjct: 121 WGTPSATSIRQLSWSEAFHVPMTDI--SDNKDFTTLSSTMEKFASESEALAYMLAEVLAE 180

Query: 181 NLGRSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELV 240
             G++S FF ENCV +TCYLRMNRYPPC  P +++GLMPHTDSDFLTIL+QDQVGGL+L+
Sbjct: 181 KSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQDQVGGLQLI 240

Query: 241 KDGKWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETV 300
           KD +WIAVKPNP+ALIINIGDLFQ WSN  YKSVEHRV+TN K ER+S AYF+CP  + V
Sbjct: 241 KDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVMTNPKVERFSTAYFMCPSYDAV 300

Query: 301 IKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFV 337
           I+  S+   YR FSFREFR QVQEDV+K+G+K+GLPRF+
Sbjct: 301 IECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRFL 334

[Close]

BLAST of CSPI01G10980 vs. ExPASy Swiss-Prot
Match: Q7XP65 (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 2.0e-90
Identity = 173/354 (48.87%), Postives = 226/354 (63.84%), Query Frame = 0


Query: 1   MDPPFHEAYKTL----------LAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAG 60
           +DPP  ++Y+ L          +A  A    G+ GA       V E DLP+VDLERLT G
Sbjct: 9   IDPPLADSYRALALLRRDRDGGIAPPAVQMVGSGGA-------VLERDLPMVDLERLTRG 68

Query: 61  KVEEVEQCKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSN 120
              E + C   +  A+ EWGFFQ+ NHG+  +L+ +MR +Q  LF+ PFE K K    + 
Sbjct: 69  GAGERKACAGAMARAASEWGFFQLTNHGVGRELMEEMRREQARLFRLPFETKEK----AG 128

Query: 121 FSAGSYRWGTPSATSITQLSWSEAFHVSLSDILGTNGSDDD--DLRSTMEEYAGKVSRLA 180
              GSYRWG P+ATS+  LSWSEAFHV L+ I G +    D   LR  M+E A  +SR+A
Sbjct: 129 LLNGSYRWGNPTATSLRHLSWSEAFHVPLASISGADCDFGDLTSLRGVMQEVAEAMSRVA 188

Query: 181 QKLAEILGENL-GR------SSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSD 240
             +A  L E L GR      ++ +F   C  +TC+LR+NRYP C      FGL+PHTDSD
Sbjct: 189 NTVAAALAEELTGRGGGGASAAPWFPAGCDETTCFLRLNRYPACPFAADTFGLVPHTDSD 248

Query: 241 FLTILHQDQVGGLELVKDGKWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKK 300
           FLT+L QDQVGGL L+KD +W+AV+P P AL++NIGDLFQ WSN+ YKSVEH+VV N+K 
Sbjct: 249 FLTVLCQDQVGGLHLMKDSRWVAVRPRPDALVVNIGDLFQAWSNNRYKSVEHKVVANAKT 308

Query: 301 ERYSIAYFLCPWSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRF 336
           +R S+AYFLCP  ++++ +  EP  YR F+F E+R +VQEDVR  G KIGLP F
Sbjct: 309 DRLSVAYFLCPSYDSLVGTCGEPSPYRAFTFGEYRKKVQEDVRTTGKKIGLPNF 351

[Close]

BLAST of CSPI01G10980 vs. ExPASy Swiss-Prot
Match: Q8LGZ9 (Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX5 PE=1 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.2e-77
Identity = 159/342 (46.49%), Postives = 212/342 (61.99%), Query Frame = 0


Query: 2   DPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKND 61
           +PP    YK L  E+        GA D     V+E +LP++DL    AG +E  + C+ D
Sbjct: 11  NPPLMSTYKHLFVEQHRLDMD-MGAID-----VDECELPVIDL----AGLMEAEQVCRAD 70

Query: 62  IITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGTP 121
           ++ A+ EWGFFQV NHG+   LL ++   Q+ +F++PF+ K  E +   FS  SYRWGTP
Sbjct: 71  MVRAASEWGFFQVTNHGVPQALLRELHDAQVAVFRRPFQEKVTE-RLLGFSPESYRWGTP 130

Query: 122 SATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENL-G 181
           +A  + QLSWSEA+H+ ++       S     R+ +EE +  +  LAQKLAEIL   L G
Sbjct: 131 TAKCLEQLSWSEAYHIPMTT---PRPSTSIRARAVIEEVSRAMYELAQKLAEILMRGLPG 190

Query: 182 RSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILH--QDQVGGLELVK 241
                 +      TC+LR+NRYPPC +    FGL PHTDSD LTI+H  QD VGGL+L+K
Sbjct: 191 AGEGETMVTTREETCFLRLNRYPPCAMAMGGFGLCPHTDSDLLTIVHQQQDTVGGLQLLK 250

Query: 242 DGKWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVI 301
            G+W+AVKP+P  LI+N+GDL Q WSND YKSVEHRV+ N+  ER+S+A+FLCP   T+I
Sbjct: 251 GGRWVAVKPSPSTLIVNVGDLLQAWSNDVYKSVEHRVMANATLERFSMAFFLCPSYHTLI 310

Query: 302 KSKS-----EPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRF 336
              S     +   YR F+F E+R Q+ EDVR  G KIGL RF
Sbjct: 311 IPSSSHVHDDDAHYRSFTFGEYRKQIMEDVRSTGRKIGLHRF 338

[Close]

BLAST of CSPI01G10980 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 2.6e-77
Identity = 142/299 (47.49%), Postives = 205/299 (68.56%), Query Frame = 0


Query: 39  LPLVDLERLTAGKVEEVEQCKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQP 98
           LP++DL  LT+G+  + ++C   ++ A+KEWGFFQ+VNHGI   +   M  ++ +LF QP
Sbjct: 40  LPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQP 99

Query: 99  FERKSKEDQFSNFSAGSYRWGTPSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTME 158
           F  K +E +FS+ S  SYRWG PSATS  Q S SEAFH+ LS++      D ++LR+ +E
Sbjct: 100 FSVKVRE-RFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEV-SRISDDRNNLRTIVE 159

Query: 159 EYAGKVSRLAQKLAEILGENLGRSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHT 218
            Y  +++R+AQ + EILG+ +  SS++F         +LR+N+Y P     ++FGL+PHT
Sbjct: 160 TYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHT 219

Query: 219 DSDFLTILHQDQVGGLELVKDGKWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTN 278
           D+ FLTIL QDQ+GGLEL  +G+WI+VKP  +AL +NIGD+FQ  SN  Y+SV HRV++ 
Sbjct: 220 DTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISP 279

Query: 279 SKKERYSIAYFLCPWSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 338
           +  ER SIA+F+CP+ ET I     P  YRRFSFRE++ Q + DV++ G K+GL RF++
Sbjct: 280 ANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRFLI 336

[Close]

BLAST of CSPI01G10980 vs. ExPASy Swiss-Prot
Match: Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 171.0 bits (432), Expect = 2.3e-41
Identity = 101/281 (35.94%), Postives = 142/281 (50.53%), Query Frame = 0


Query: 34  VEEWDLPLVDLERLTAGKVEEVEQCKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQIE 93
           V E D+PL+DL+ L +     ++  +  I  A K+ GFF VVNHGIS +L++        
Sbjct: 56  VLELDVPLIDLQNLLSDPSSTLDASRL-ISEACKKHGFFLVVNHGISEELISDAHEYTSR 115

Query: 94  LFKQPFERKSKEDQFSNFSAGSYRWGTPSATSITQLSWSEAFHVSLSDILGTNGSDDD-- 153
            F  P   K +  + S  S G Y        S T+L W E       D +  + S  D  
Sbjct: 116 FFDMPLSEKQRVLRKSGESVG-YASSFTGRFS-TKLPWKETLSFRFCDDMSRSKSVQDYF 175

Query: 154 ---------DLRSTMEEYAGKVSRLAQKLAEILGENLGRSSKFFVENCVPSTCYLRMNRY 213
                          +EY   +S L+ K+ E+LG +LG    +F E    +   +R+N Y
Sbjct: 176 CDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYFREFFEENDSIMRLNYY 235

Query: 214 PPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAVKPNPQALIINIGDLFQV 273
           PPC  P    G  PH D   LTILHQD V GL++  + +W +++PNP+A ++NIGD F  
Sbjct: 236 PPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIRPNPKAFVVNIGDTFMA 295

Query: 274 WSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKSKSE 304
            SND YKS  HR V NS+ ER S+A+FLCP  + V+    E
Sbjct: 296 LSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRE 333

[Close]

BLAST of CSPI01G10980 vs. ExPASy TrEMBL
Match: S0CRC1 (GA 2-oxidase (Fragment) OS=Cucumis sativus OX=3659 GN=GA2ox5 PE=2 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 5.4e-195
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60
           MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN
Sbjct: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60

Query: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120
           DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT
Sbjct: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120

Query: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 180
           PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLG
Sbjct: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 180

Query: 181 RSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 240
           RSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG
Sbjct: 181 RSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 240

Query: 241 KWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS 300
           KWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS
Sbjct: 241 KWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS 300

Query: 301 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 338
           KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL
Sbjct: 301 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 337

[Close]

BLAST of CSPI01G10980 vs. ExPASy TrEMBL
Match: A0A5A7UM12 (Gibberellin 2-beta-dioxygenase 8 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G006030 PE=3 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 2.7e-178
Identity = 314/337 (93.18%), Postives = 319/337 (94.66%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60
           MDPPFHEAYKTLLAE AA    AKG SDVV   VEEWDLP+VDL RLT GKVEE E+CKN
Sbjct: 1   MDPPFHEAYKTLLAETAAE---AKGTSDVV--TVEEWDLPVVDLGRLTGGKVEEEEECKN 60

Query: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120
           DII ASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT
Sbjct: 61  DIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120

Query: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 180
           PSATSITQLSWSEAFHVSLSDILG+N   DDDLRSTMEEYAGKVSRLAQKLAEILGENLG
Sbjct: 121 PSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 180

Query: 181 RSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 240
           RSSKFFVENCVPSTCYLRMNRYPPC V GQIFGLMPHTDSDFLTILHQDQVGGLELVKDG
Sbjct: 181 RSSKFFVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 240

Query: 241 KWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS 300
           KWIAVKPNPQALI+NIGDLFQVWSNDEYKSVEHRVVTNS+KERYSIAYFLCPWSETVIKS
Sbjct: 241 KWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKS 300

Query: 301 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 338
           KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL
Sbjct: 301 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 332

[Close]

BLAST of CSPI01G10980 vs. ExPASy TrEMBL
Match: A0A1S3B3F3 (gibberellin 2-beta-dioxygenase 8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485556 PE=3 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 2.7e-178
Identity = 314/337 (93.18%), Postives = 319/337 (94.66%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60
           MDPPFHEAYKTLLAE AA    AKG SDVV   VEEWDLP+VDL RLT GKVEE E+CKN
Sbjct: 46  MDPPFHEAYKTLLAETAAE---AKGTSDVV--TVEEWDLPVVDLGRLTGGKVEEEEECKN 105

Query: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120
           DII ASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT
Sbjct: 106 DIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 165

Query: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 180
           PSATSITQLSWSEAFHVSLSDILG+N   DDDLRSTMEEYAGKVSRLAQKLAEILGENLG
Sbjct: 166 PSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 225

Query: 181 RSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 240
           RSSKFFVENCVPSTCYLRMNRYPPC V GQIFGLMPHTDSDFLTILHQDQVGGLELVKDG
Sbjct: 226 RSSKFFVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 285

Query: 241 KWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS 300
           KWIAVKPNPQALI+NIGDLFQVWSNDEYKSVEHRVVTNS+KERYSIAYFLCPWSETVIKS
Sbjct: 286 KWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKS 345

Query: 301 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 338
           KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL
Sbjct: 346 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 377

[Close]

BLAST of CSPI01G10980 vs. ExPASy TrEMBL
Match: A0A1S3B309 (gibberellin 2-beta-dioxygenase 8 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485556 PE=3 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 2.7e-178
Identity = 314/337 (93.18%), Postives = 319/337 (94.66%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60
           MDPPFHEAYKTLLAE AA    AKG SDVV   VEEWDLP+VDL RLT GKVEE E+CKN
Sbjct: 14  MDPPFHEAYKTLLAETAAE---AKGTSDVV--TVEEWDLPVVDLGRLTGGKVEEEEECKN 73

Query: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120
           DII ASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT
Sbjct: 74  DIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 133

Query: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 180
           PSATSITQLSWSEAFHVSLSDILG+N   DDDLRSTMEEYAGKVSRLAQKLAEILGENLG
Sbjct: 134 PSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 193

Query: 181 RSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 240
           RSSKFFVENCVPSTCYLRMNRYPPC V GQIFGLMPHTDSDFLTILHQDQVGGLELVKDG
Sbjct: 194 RSSKFFVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 253

Query: 241 KWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS 300
           KWIAVKPNPQALI+NIGDLFQVWSNDEYKSVEHRVVTNS+KERYSIAYFLCPWSETVIKS
Sbjct: 254 KWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKS 313

Query: 301 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 338
           KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL
Sbjct: 314 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 345

[Close]

BLAST of CSPI01G10980 vs. ExPASy TrEMBL
Match: A0A6J1F422 (gibberellin 2-beta-dioxygenase 8 OS=Cucurbita moschata OX=3662 GN=LOC111440045 PE=3 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 5.6e-160
Identity = 283/338 (83.73%), Postives = 306/338 (90.53%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAG-KVEEVEQCK 60
           MDPPFHEAYK+LLAE A    G     D V   V+EW+LP+VDL RL  G +V EVEQCK
Sbjct: 13  MDPPFHEAYKSLLAETAVERGG-----DAV--AVKEWELPVVDLRRLRDGAEVGEVEQCK 72

Query: 61  NDIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWG 120
           NDII AS+EWGFFQVVNHG+S+QLL KMRAKQI LFKQPFERKSKED F NFSAGSYRWG
Sbjct: 73  NDIIRASQEWGFFQVVNHGVSSQLLGKMRAKQIGLFKQPFERKSKEDMFLNFSAGSYRWG 132

Query: 121 TPSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENL 180
           TP+ATS+TQLSWSEAFHVSLSDILG+NGS  DDLRSTMEE+AGKVSRLAQ+LAEILGENL
Sbjct: 133 TPTATSLTQLSWSEAFHVSLSDILGSNGS--DDLRSTMEEFAGKVSRLAQELAEILGENL 192

Query: 181 GRSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKD 240
           GRSSKFFV+NCVPSTCYLRMNRYPPC VPG++FGLMPHTDSDFLTILHQDQVGGLELVKD
Sbjct: 193 GRSSKFFVDNCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKD 252

Query: 241 GKWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIK 300
           G+WIAVKPNP+ALIINIGDLFQ WSN+ YKSVEHRVVTNSK ER+SIAYF CPWSETVIK
Sbjct: 253 GEWIAVKPNPEALIINIGDLFQAWSNNVYKSVEHRVVTNSKLERFSIAYFFCPWSETVIK 312

Query: 301 SKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 338
           SK EPGVYRRFSFREFRNQVQEDVRK+G+KIGLP+FVL
Sbjct: 313 SKCEPGVYRRFSFREFRNQVQEDVRKHGFKIGLPKFVL 341

[Close]

BLAST of CSPI01G10980 vs. NCBI nr
Match: XP_004153456.2 (gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis sativus] >XP_031742868.1 gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis sativus] >KAE8652673.1 hypothetical protein Csa_013900 [Cucumis sativus])

HSP 1 Score: 689.9 bits (1779), Expect = 1.1e-194
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60
           MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN
Sbjct: 15  MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 74

Query: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120
           DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT
Sbjct: 75  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 134

Query: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 180
           PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLG
Sbjct: 135 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 194

Query: 181 RSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 240
           RSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG
Sbjct: 195 RSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 254

Query: 241 KWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS 300
           KWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS
Sbjct: 255 KWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS 314

Query: 301 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 338
           KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL
Sbjct: 315 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 351

[Close]

BLAST of CSPI01G10980 vs. NCBI nr
Match: XP_031742874.1 (gibberellin 2-beta-dioxygenase 8 isoform X2 [Cucumis sativus] >CCL98007.1 GA 2-oxidase, partial [Cucumis sativus])

HSP 1 Score: 689.9 bits (1779), Expect = 1.1e-194
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60
           MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN
Sbjct: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60

Query: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120
           DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT
Sbjct: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120

Query: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 180
           PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLG
Sbjct: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 180

Query: 181 RSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 240
           RSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG
Sbjct: 181 RSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 240

Query: 241 KWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS 300
           KWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS
Sbjct: 241 KWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS 300

Query: 301 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 338
           KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL
Sbjct: 301 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 337

[Close]

BLAST of CSPI01G10980 vs. NCBI nr
Match: XP_008441451.1 (PREDICTED: gibberellin 2-beta-dioxygenase 8 isoform X2 [Cucumis melo])

HSP 1 Score: 634.4 bits (1635), Expect = 5.5e-178
Identity = 314/337 (93.18%), Postives = 319/337 (94.66%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60
           MDPPFHEAYKTLLAE AA    AKG SDVV   VEEWDLP+VDL RLT GKVEE E+CKN
Sbjct: 14  MDPPFHEAYKTLLAETAAE---AKGTSDVV--TVEEWDLPVVDLGRLTGGKVEEEEECKN 73

Query: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120
           DII ASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT
Sbjct: 74  DIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 133

Query: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 180
           PSATSITQLSWSEAFHVSLSDILG+N   DDDLRSTMEEYAGKVSRLAQKLAEILGENLG
Sbjct: 134 PSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 193

Query: 181 RSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 240
           RSSKFFVENCVPSTCYLRMNRYPPC V GQIFGLMPHTDSDFLTILHQDQVGGLELVKDG
Sbjct: 194 RSSKFFVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 253

Query: 241 KWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS 300
           KWIAVKPNPQALI+NIGDLFQVWSNDEYKSVEHRVVTNS+KERYSIAYFLCPWSETVIKS
Sbjct: 254 KWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKS 313

Query: 301 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 338
           KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL
Sbjct: 314 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 345

[Close]

BLAST of CSPI01G10980 vs. NCBI nr
Match: KAA0056882.1 (gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis melo var. makuwa] >TYJ99385.1 gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 634.4 bits (1635), Expect = 5.5e-178
Identity = 314/337 (93.18%), Postives = 319/337 (94.66%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60
           MDPPFHEAYKTLLAE AA    AKG SDVV   VEEWDLP+VDL RLT GKVEE E+CKN
Sbjct: 1   MDPPFHEAYKTLLAETAAE---AKGTSDVV--TVEEWDLPVVDLGRLTGGKVEEEEECKN 60

Query: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120
           DII ASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT
Sbjct: 61  DIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120

Query: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 180
           PSATSITQLSWSEAFHVSLSDILG+N   DDDLRSTMEEYAGKVSRLAQKLAEILGENLG
Sbjct: 121 PSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 180

Query: 181 RSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 240
           RSSKFFVENCVPSTCYLRMNRYPPC V GQIFGLMPHTDSDFLTILHQDQVGGLELVKDG
Sbjct: 181 RSSKFFVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 240

Query: 241 KWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS 300
           KWIAVKPNPQALI+NIGDLFQVWSNDEYKSVEHRVVTNS+KERYSIAYFLCPWSETVIKS
Sbjct: 241 KWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKS 300

Query: 301 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 338
           KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL
Sbjct: 301 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 332

[Close]

BLAST of CSPI01G10980 vs. NCBI nr
Match: XP_008441442.1 (PREDICTED: gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis melo])

HSP 1 Score: 634.4 bits (1635), Expect = 5.5e-178
Identity = 314/337 (93.18%), Postives = 319/337 (94.66%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGASDVVLTVVEEWDLPLVDLERLTAGKVEEVEQCKN 60
           MDPPFHEAYKTLLAE AA    AKG SDVV   VEEWDLP+VDL RLT GKVEE E+CKN
Sbjct: 46  MDPPFHEAYKTLLAETAAE---AKGTSDVV--TVEEWDLPVVDLGRLTGGKVEEEEECKN 105

Query: 61  DIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 120
           DII ASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT
Sbjct: 106 DIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 165

Query: 121 PSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 180
           PSATSITQLSWSEAFHVSLSDILG+N   DDDLRSTMEEYAGKVSRLAQKLAEILGENLG
Sbjct: 166 PSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 225

Query: 181 RSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 240
           RSSKFFVENCVPSTCYLRMNRYPPC V GQIFGLMPHTDSDFLTILHQDQVGGLELVKDG
Sbjct: 226 RSSKFFVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 285

Query: 241 KWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKS 300
           KWIAVKPNPQALI+NIGDLFQVWSNDEYKSVEHRVVTNS+KERYSIAYFLCPWSETVIKS
Sbjct: 286 KWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKS 345

Query: 301 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 338
           KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL
Sbjct: 346 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 377

[Close]

BLAST of CSPI01G10980 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 423.3 bits (1087), Expect = 1.8e-118
Identity = 207/339 (61.06%), Postives = 255/339 (75.22%), Query Frame = 0


Query: 1   MDPPFHEAYKTLLAERAAAATGAKGA---SDVVLTVVEEWDLPLVDLERLTAGKVEEVEQ 60
           MDPPF+E Y  LL  +             S  V  VVEE +LP++D+ RL  G  EE E+
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDNDVSEIPFSFSVTAVVEEVELPVIDVSRLIDGAEEEREK 60

Query: 61  CKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYR 120
           CK  I  AS+EWGFFQV+NHGIS  +L KMR +QI +F++PF++KSK ++   FSAGSYR
Sbjct: 61  CKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEK---FSAGSYR 120

Query: 121 WGTPSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGE 180
           WGTPSATSI QLSWSEAFHV ++DI  ++  D   L STME++A +   LA  LAE+L E
Sbjct: 121 WGTPSATSIRQLSWSEAFHVPMTDI--SDNKDFTTLSSTMEKFASESEALAYMLAEVLAE 180

Query: 181 NLGRSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELV 240
             G++S FF ENCV +TCYLRMNRYPPC  P +++GLMPHTDSDFLTIL+QDQVGGL+L+
Sbjct: 181 KSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQDQVGGLQLI 240

Query: 241 KDGKWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETV 300
           KD +WIAVKPNP+ALIINIGDLFQ WSN  YKSVEHRV+TN K ER+S AYF+CP  + V
Sbjct: 241 KDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVMTNPKVERFSTAYFMCPSYDAV 300

Query: 301 IKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFV 337
           I+  S+   YR FSFREFR QVQEDV+K+G+K+GLPRF+
Sbjct: 301 IECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRFL 334

[Close]

BLAST of CSPI01G10980 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 290.4 bits (742), Expect = 1.8e-78
Identity = 142/299 (47.49%), Postives = 205/299 (68.56%), Query Frame = 0


Query: 39  LPLVDLERLTAGKVEEVEQCKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQP 98
           LP++DL  LT+G+  + ++C   ++ A+KEWGFFQ+VNHGI   +   M  ++ +LF QP
Sbjct: 40  LPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQP 99

Query: 99  FERKSKEDQFSNFSAGSYRWGTPSATSITQLSWSEAFHVSLSDILGTNGSDDDDLRSTME 158
           F  K +E +FS+ S  SYRWG PSATS  Q S SEAFH+ LS++      D ++LR+ +E
Sbjct: 100 FSVKVRE-RFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEV-SRISDDRNNLRTIVE 159

Query: 159 EYAGKVSRLAQKLAEILGENLGRSSKFFVENCVPSTCYLRMNRYPPCHVPGQIFGLMPHT 218
            Y  +++R+AQ + EILG+ +  SS++F         +LR+N+Y P     ++FGL+PHT
Sbjct: 160 TYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHT 219

Query: 219 DSDFLTILHQDQVGGLELVKDGKWIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRVVTN 278
           D+ FLTIL QDQ+GGLEL  +G+WI+VKP  +AL +NIGD+FQ  SN  Y+SV HRV++ 
Sbjct: 220 DTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISP 279

Query: 279 SKKERYSIAYFLCPWSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 338
           +  ER SIA+F+CP+ ET I     P  YRRFSFRE++ Q + DV++ G K+GL RF++
Sbjct: 280 ANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRFLI 336

[Close]

BLAST of CSPI01G10980 vs. TAIR 10
Match: AT5G58660.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 182.2 bits (461), Expect = 7.0e-46
Identity = 122/335 (36.42%), Postives = 176/335 (52.54%), Query Frame = 0


Query: 30  VLTVVEEWDLPLVDLERLTAGKVEEVEQCKNDIITASKEWGFFQVVNHGISNQLLAKMRA 89
           V+   ++ ++P++DLERL           K  +  A KEWG F++ NHG+   L ++++ 
Sbjct: 25  VIVQGKDINIPVIDLERLD----------KEILREACKEWGIFRLENHGVPLALTSRLQE 84

Query: 90  KQIELFKQPFERKSKEDQFSNFSAGSYRWGTPS----------ATSITQLSWSEAFHVSL 149
               L   PFE+K +E   +  S  SY WGTP+              + L+  E F+V L
Sbjct: 85  ISESLLSLPFEKK-RELFAAVKSPLSYFWGTPALNRSGDALKRGAQASNLTMLEGFNVPL 144

Query: 150 SDI-----LGTNGSDDDD---------LRSTMEEYAGKVSRLAQKLAEILGE--NLGRSS 209
           S +     L T+   DDD          R  MEEY   ++R+A  L E + +  NL  S 
Sbjct: 145 SSLSSLSKLPTSTCCDDDAQEEPKLESFRVLMEEYGKHITRIAVSLFEAIAQTLNLELSG 204

Query: 210 KFFVENCVPSTCYLRMNRYPPC--HVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGK 269
               E    ST  +R+ RYP        +  G+  HTDS  ++IL +D+ GGLE++K  +
Sbjct: 205 NRRSEYLSESTGLIRVYRYPQSSEEAAREALGMEVHTDSSVISILREDESGGLEIMKGEE 264

Query: 270 WIAVKPNPQALIINIGDLFQVWSNDEYKSVEHRV-VTNSKKERYSIAYFLCPWSETVIKS 329
           W  VKP    LI+N+GD+ Q  S+DEYKSV HRV   N K ER+S+ YF+ P  + VIKS
Sbjct: 265 WFCVKPVANTLIVNLGDMMQAISDDEYKSVTHRVKKRNRKTERHSVCYFVFPKRDCVIKS 324

Query: 330 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRF 336
            +    Y+ F++ +F  QVQ DV+  G KIGLPRF
Sbjct: 325 SN----YKLFTYSDFEAQVQADVQSLGTKIGLPRF 344

[Close]

BLAST of CSPI01G10980 vs. TAIR 10
Match: AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 171.0 bits (432), Expect = 1.6e-42
Identity = 101/281 (35.94%), Postives = 142/281 (50.53%), Query Frame = 0


Query: 34  VEEWDLPLVDLERLTAGKVEEVEQCKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQIE 93
           V E D+PL+DL+ L +     ++  +  I  A K+ GFF VVNHGIS +L++        
Sbjct: 56  VLELDVPLIDLQNLLSDPSSTLDASRL-ISEACKKHGFFLVVNHGISEELISDAHEYTSR 115

Query: 94  LFKQPFERKSKEDQFSNFSAGSYRWGTPSATSITQLSWSEAFHVSLSDILGTNGSDDD-- 153
            F  P   K +  + S  S G Y        S T+L W E       D +  + S  D  
Sbjct: 116 FFDMPLSEKQRVLRKSGESVG-YASSFTGRFS-TKLPWKETLSFRFCDDMSRSKSVQDYF 175

Query: 154 ---------DLRSTMEEYAGKVSRLAQKLAEILGENLGRSSKFFVENCVPSTCYLRMNRY 213
                          +EY   +S L+ K+ E+LG +LG    +F E    +   +R+N Y
Sbjct: 176 CDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYFREFFEENDSIMRLNYY 235

Query: 214 PPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAVKPNPQALIINIGDLFQV 273
           PPC  P    G  PH D   LTILHQD V GL++  + +W +++PNP+A ++NIGD F  
Sbjct: 236 PPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIRPNPKAFVVNIGDTFMA 295

Query: 274 WSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKSKSE 304
            SND YKS  HR V NS+ ER S+A+FLCP  + V+    E
Sbjct: 296 LSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRE 333

[Close]

BLAST of CSPI01G10980 vs. TAIR 10
Match: AT5G07200.1 (gibberellin 20-oxidase 3 )

HSP 1 Score: 169.5 bits (428), Expect = 4.7e-42
Identity = 99/291 (34.02%), Postives = 140/291 (48.11%), Query Frame = 0


Query: 32  TVVEEWDLPLVDLERLTAGKVEEVEQCKNDIITASKEWGFFQVVNHGISNQLLAKMRAKQ 91
           T V+   +PL+DL    +G      +    +  A+ + GFF + NHG+   LL++     
Sbjct: 51  TDVQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHM 110

Query: 92  IELFKQPFERKSKEDQFSNFSAGSYRWGTPS--ATSI-----TQLSWSEAFHVSLSD--- 151
              FK P   K K  +         +WG  S  A+S      ++L W E      S    
Sbjct: 111 DSFFKAPACEKQKAQR---------KWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEK 170

Query: 152 ---------ILGTNGSDDDDLRSTMEEYAGKVSRLAQKLAEILGENLGRSSKFFVENCVP 211
                    +    G   +D     +EYA  ++ L+ K+ E+LG +LG   ++F E    
Sbjct: 171 IHSQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFED 230

Query: 212 STCYLRMNRYPPCHVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAVKPNPQAL 271
           S    R+N YP C  P    G  PH D   LTILHQDQVGGL++  D KW ++ PNP A 
Sbjct: 231 SDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAF 290

Query: 272 IINIGDLFQVWSNDEYKSVEHRVVTNSKKERYSIAYFLCPWSETVIKSKSE 304
           ++NIGD F   +N  YKS  HR V NS++ER + A+FLCP  E V+K   E
Sbjct: 291 VVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEE 332

The following BLAST results are available for this feature:

BLAST of CSPI01G10980 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49561	2.5e-117	61.06	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ...	[more]
Q7XP65	2.0e-90	48.87	Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
Q8LGZ9	1.2e-77	46.49	Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
Q9C6I4	2.6e-77	47.49	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ...	[more]
Q39110	2.3e-41	35.94	Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2	[more]

BLAST of CSPI01G10980 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
S0CRC1	5.4e-195	100.00	GA 2-oxidase (Fragment) OS=Cucumis sativus OX=3659 GN=GA2ox5 PE=2 SV=1	[more]
A0A5A7UM12	2.7e-178	93.18	Gibberellin 2-beta-dioxygenase 8 isoform X1 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3B3F3	2.7e-178	93.18	gibberellin 2-beta-dioxygenase 8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485...	[more]
A0A1S3B309	2.7e-178	93.18	gibberellin 2-beta-dioxygenase 8 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485...	[more]
A0A6J1F422	5.6e-160	83.73	gibberellin 2-beta-dioxygenase 8 OS=Cucurbita moschata OX=3662 GN=LOC111440045 P...	[more]

BLAST of CSPI01G10980 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004153456.2	1.1e-194	100.00	gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis sativus] >XP_031742868.1 gi...	[more]
XP_031742874.1	1.1e-194	100.00	gibberellin 2-beta-dioxygenase 8 isoform X2 [Cucumis sativus] >CCL98007.1 GA 2-o...	[more]
XP_008441451.1	5.5e-178	93.18	PREDICTED: gibberellin 2-beta-dioxygenase 8 isoform X2 [Cucumis melo]	[more]
KAA0056882.1	5.5e-178	93.18	gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis melo var. makuwa] >TYJ99385...	[more]
XP_008441442.1	5.5e-178	93.18	PREDICTED: gibberellin 2-beta-dioxygenase 8 isoform X1 [Cucumis melo]	[more]

BLAST of CSPI01G10980 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21200.1	1.8e-118	61.06	gibberellin 2-oxidase 8	[more]
AT1G50960.1	1.8e-78	47.49	gibberellin 2-oxidase 7	[more]
AT5G58660.1	7.0e-46	36.42	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G25420.1	1.6e-42	35.94	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G07200.1	4.7e-42	34.02	gibberellin 20-oxidase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 39..140 e-value: 1.1E-15 score: 58.5
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 198..292 e-value: 4.9E-30 score: 104.0
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 37..329 e-value: 6.9E-81 score: 274.0
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 28..330
None	No IPR available	PANTHER	PTHR47990:SF13	GIBBERELLIN 2-BETA-DIOXYGENASE 8-LIKE	coord: 28..330
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 33..321
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 193..292 score: 15.63971

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G10980.1	CSPI01G10980.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009685	gibberellin metabolic process
molecular_function	GO:0052635	C-20 gibberellin 2-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding