CSPI01G05150 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G05150
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionD-cysteine desulfhydrase
LocationChr1: 3370086 .. 3375489 (+)
RNA-Seq ExpressionCSPI01G05150
SyntenyCSPI01G05150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGACAAAAGGCAATCGAAATTGGAAATAAAACAAGCGGATGCGGATGCGAGGCCGGTAATGGAATCGAAAGGGGCGGATATTTTCACTGGCGGACTGGCGAAATGTTGAGCGGTGGCAAGTTCCAACTGTGTCTACTCACCGCAGCACCATCCAACTTGGCCTCATCGTCATCGTCGTCGTCATCGCTCTTCTCAACTTCTCTAATTCATCCAAGAAGGTTCGACAACATCAACAAGCGTTTCTCCTTTCCTCATCCATCAACTCCGAGTTTAGATTGCATTCGTTGCTGTATTTCACAATCTCAGTCCATGGAGATCAACACTGAAAGCAAGCAACACGACGAGGTCCCTAACAGCCTCAACTTCCTCTCCGTCAGACCTTACGTCCCTCCGTCCTGGGCCTCACACCTTAATCCAATCCCCACCCACCTCTCTTCTCTTGCCCGTGTAAGTTTACTCTTCCTAGTTTCTTTTCCCGCTTGCCTCTTCCTGGGTCTATTGCAATTATTGCTTCCTTAAGAACATCGTTGTAGTTGGTTGCTGATTACAATTTTGGCATCAGCTTCCCACTCCCATTCACAAGTGGAACCTTCCTAATCTGCCCAACAACACAGAGGTCTGGCTCAAGGTACATCATTCTGACTCAATTTCAACTATTTTCTTTTACAGAAACTCGTTTGATTGTCTTCCCGCCAACGTGGTAATGGATGCGTTACATCCATCATTTCTCTGTTAGAGTGTTTGTACATTTACTGTTTATTGTTTCTCTGTGATACGTTTTGTGCTCCACTCTATTTATTCAATAAGATGAACTCTCTTTAATTTGATAGGCTATTACATCATGCACGGCACTTTTAAGAATTTTGCCCCGTTTCATACTTCCTCAGAACATAATTTTGCCGGTGGCCTTTCTGCTGATTTACGGAACTCTGCTTTCCGTGTCCGAAAATTGATTGAGAGTTCTATGATCAGATTGACAGTTTTTCGTTTGAAGAGAATTTTGTATGTGAAACTTTCAGGCGCATGAGCAACATTAAAGAACAATATAGGACCATAAAATAAATAATATATCAACTGTTGGAGGGAGTACATAAGCTTTTCTGGCTTCACCATGCCTTCACTTTAATGGTATTTTCATATTTCTTCTATCCACAATTGAAGTATCTGTTCATGCGTGACTGCACTTTTGCCCAAGGACTTCAGAGTCACACTTCGATGTCCTTATCAATAAAATATTTCAATTCCAATTTTGAGTCCCTCTTAGATATAATATTAAATCTGCCATCATCCACTAGTTTAAGCTATTGGGCCGAGTTGGTGATTTAAGATGGTATTAAAGTAGTGTGGTTTGGGATGTCCTGGTTCAAGACTCTGCATTGTTGTTTCCTTTTAATTTAATATTCATTTTCATTTTTTGGATATTTATTCATATTTCTAACCCACAAGAGAGGGGAATTAGATATAATATCTAATTTTGTTTCTAAATGTACCTAGCTTAGTTATTCTTGTTTAACTTTTTTTTTCCTTATTTACTTGTTTACACTAATGAAATATTCCTGGCAAAATGAATACAGCGTGATGATCTTTCAGGAATGCAATTGAGTGGAAATAAAGTCAGAAAATTAGAATTCTTGATAGCAGATGCTTTGCAGCAGGGTGCTGACTGTATTATAACTATAGGAGGCATCCAAAGCAATCACTGTCGTGCTACTGCTGTGGCTGCAAAGTATTTCAATCTTGACACATATCTAATTCTACGCACTTCAAAGGTTATCATTATTTTCCCTCTGTTTTCAACTTATTTACTTAAAATATGCTTGTTTTGCATTTTTTAAAATGACCTCTCTCAGGATTTTTCTAAGTTATGACTTGATAACCAATGTTACGTCCCTGATGCAGGTCCTTGTGAATGAAAACCCCGGATTGACTGGAAATCTTCTTGTTGAGCGGTTAGTCGGAGCTCATGTCGAACTTATCTCAAAAGAAGAGTATGCAAAAATTGGAAGCGTGGTTTGTTGATTTCTTCTATGTTACCATTTCTTTTTTTGGTTTTTAGAAGCAGTTCATTCTCCTATTGATCAGTTGACAGCTTCTCTAATTCTAGACTGATAGATGTTATAAGAATCATGGAGCTCACTAGTTTCTCTTTTCTCCTAAACAAAAGTAACTCCTTGAGAGCTTATTACCTTCTTTTGGTAATTATAATAGGTAACAATTTTAGGGATAATAATTAAGTATATAGCAACATTTTTCAAAAAATTGCAAATATAACGAAATCTATCAGCGATAAACTTCTATTTGCGAAATGTCTATCAACTAGAATTCTATCATTGACAGACTCTGATAAGTTTTGCTATATTTATAATTTTATAAAAATGTTGCTATAGATGTTAATGTTTTGAATCTAATTAGGTTTAGCTATATTTACAATTTTTTTAAAATGTTGCTATGTATGCCTAATATTTTAAATCTAATTGCCATATTTTCAACTGTTTTTTTCTTTTTATATATTGCAGGATATTTGACTAATGCTATTGCTAAGTTTACTGTTACTCTTATGAGTTTGTAAGGCTCATGAAGCAATGTCTGCGTATTTTTTTATGATCATGTTCATATGCTTTTGGCACCTGCTAATATTGTGTTAAACATACTTGAATCTTGTTTCAGCGTTCTAGTTCTTTGATATTAGGTAGTCCTTCTTTCACTTCAAATTGGCTAGAGTTTCAGCATTTACCTGGTTGTTACGAGACAAAATATTCTGCCAATTTCTTTTACATACCAAATAGTAGATTCACGAGGGTTTCGTTTTCCTCACGTCCTTCTTTACTTAATCAATATTGATATATTTTTTTTGTTTTGCATTTTATTCTGATAAGAAGTGTATTGCTTTAATCGACTGTGGTTACTCTTTACCCACTTATTTACTTGGGATATGACGCTCTTCACTCTTCTATACATTTCCATTGCTTTGTTTTTAGTTTGTTTATTTCAACATCTGTTTGATGCTTTTTCTTCTTTGGTTCCAATTTCAGGCACTAACAGATTTTCTAAAGTCAAAATTAGTTGCTGAAGGAAGAAAGCCATATGTTATTCCTGTTGGAGGTTCAAATTCCCTTGGAACTTGGTAAGAATTGGTTGTGCTTGTTTCCTCTTGTCCACTATATTAGGTGTGGTTGGATTTGATGATGATAGTATGCTGGCTGTTCTCTGGAAAAGAATAGAACAAGCATGGTTAAAGAATAGAACAAGCATGGTTAAGGAGCATAAATAATCTTCTAGATTTCATTCTAGATAGTCGTTGGTTTTCTTCCTTCTTCCTTTCAAACTTCTACTTCACATTGGGATCATATTCTACCACCATTTTTTATTCTTCCTCGAGATTTTTGCTTTTCTGATAGTTCTTTTACTTCATCACAGTCTTGTTAGGTTTTTAACTTTCAATTTCTGATTCATAAGAGGATTTTTTATTGTTCTTTATTCTTGTAATTATGTTCGTATTAAAATATGTCTTTGTTTTATGGGAAAAATTTTTTTTGCATTCGTTGCAACAAAAAAGCATCCAGCTCCACTTTATTTCTTCTTCTCCCTTCTTGAACCTATTTTGCCATGCTAGTACGCCCCGCCCCCACCCCCCTTGAGAACAGTAATATGTGTAAATGCTTGAGTATTAAGTTTATTGATTTACTGTCAGTTAAGGAGACATTATCATTCGAATATATATTGCATAATAATCATCTGTACATCTTCTCCGTCTCTCGCTTTCTCTCTGTTATTATAATTTTATATAGCAAATGTGTTTTGGTTATGTTCAGGGGCTACATCGAAGCAATTAGGGAGCTTGAACAGCAACTGGATTCCGGAAATGGCAAGATTAAATTTGATGATATTGTAGTAGCTTGTGGCAGGTTCTTCTTCTTCTTTTGGTCGATGGGATCACTTGGTCCATTTTCCCTGTTTAGTTTCTTTTTATTAACTAGGGCGTGGGCTTCATGCTGAATAATTCACAAGTTTTCTGTGACAGGTGATAAGAATAATTTTTTTAATCTTTGATGTTTTGCTTTTGTTTATTTTCAGTGGGGGCACAGTCGCTGGTTTATCATTGGGATCATGGTTGAGCACATTGAAGACAAAAGTATGATATCTTTTTTTCTTTCTAAATTTTTTGCATTGTTGCTCTTTACATTTTAGTTCCTCACATTGTAAGTTTGTTGAATCTACAGATTCGTGCGTTCTCTGTTTGTGATGATCCTGATTACTTTTACGAGTTTGTTCAAGGTTTACTTGATGGGCTTCATGCTGGTGTCGACTCACGCGATATAGTTGAAATACAAAATGTAAGTAGGACTTTTAGTATGAAAGGTTGTTGGTTTGCTCACGTTTGGCAGCACCATTTATGTTGTGATGTTGAGGTGAAACCTTTCCCGTAGTGTCATAGTTCTTTGGAGCCTAATCTCCGATCTAGAGATACAAAGCACATCTTTCTTCTTCGGTTCTTCCCTTCTCTTTGTGATAGTTTAGATCTTTCATCTCCATAAAAGAAACTTGAGAGGAACTCTCTTCTTCTCGTTCTCTGATTAGGCAAAGGGTCTTGGCTATGCAATCAACACGCCTGATGAGCTTAATTTCGTAAAAGAAGTTGCAGAATCAACCGGCGTTGTTCTTGATCCAGTATACAGGTATGTTGGTATTTATAACTAGTCAAGTTCATAGTTTATGCTCACAATTGACGACAGACCATCTTGTATTTCTTTTTAAAATTGTAGTTCATCTTGTAAACCGTTGATATGTTTGCATGGTTTTTATACGAAGGTATGCATAGAGTAAAATGTAATGGCTTTGTTTTTTTCCCCTTTCTCTATGACATTATTTTCAGCGGGAAAGCAGCTTATGGTATGATGAAAGACATGGCAGAAAATCCAAAGAAGTGGGAAGGCAGGAAGATTCTGTTCATTCACACAGGAGGGTTGTTGGGACTATATGACAAAGCTGATCAGATCAATTCAACGCTGGGGAAATGGCATCGTTTGGACGTTAATGAGACTGTGCCACGAATTGATGGAGTTGGGAAAATGTTCTAGAACAACTCGGATCAAAAAGTGTAGCCACTATTGAAAGACGGGTGCTTTAAGATTTCTTATCAGACATTTTAATTATTAGGGGTTCAAATATATTGTTATGGATTATTGATAATCCAATAATATATACCTTGTCTTTATTTTGTATTTGCTAGACGTATCATGAATGCCAATTCGTACCTTATTAGTTGAATAAGGCACCGATTGCTATTTTGAATATCGATAGTTCCGTATGTAAATTGTTATTGAAAAAAACCTTGAAGCAAGGAAGTGTATAGTTGGTATTTTCCTTTTGTGTTTTCTTTTTAAAGACAACATAATTTTCAGGG

mRNA sequence

CGACAAAAGGCAATCGAAATTGGAAATAAAACAAGCGGATGCGGATGCGAGGCCGGTAATGGAATCGAAAGGGGCGGATATTTTCACTGGCGGACTGGCGAAATGTTGAGCGGTGGCAAGTTCCAACTGTGTCTACTCACCGCAGCACCATCCAACTTGGCCTCATCGTCATCGTCGTCGTCATCGCTCTTCTCAACTTCTCTAATTCATCCAAGAAGGTTCGACAACATCAACAAGCGTTTCTCCTTTCCTCATCCATCAACTCCGAGTTTAGATTGCATTCGTTGCTGTATTTCACAATCTCAGTCCATGGAGATCAACACTGAAAGCAAGCAACACGACGAGGTCCCTAACAGCCTCAACTTCCTCTCCGTCAGACCTTACGTCCCTCCGTCCTGGGCCTCACACCTTAATCCAATCCCCACCCACCTCTCTTCTCTTGCCCGTCTTCCCACTCCCATTCACAAGTGGAACCTTCCTAATCTGCCCAACAACACAGAGGTCTGGCTCAAGCGTGATGATCTTTCAGGAATGCAATTGAGTGGAAATAAAGTCAGAAAATTAGAATTCTTGATAGCAGATGCTTTGCAGCAGGGTGCTGACTGTATTATAACTATAGGAGGCATCCAAAGCAATCACTGTCGTGCTACTGCTGTGGCTGCAAAGTATTTCAATCTTGACACATATCTAATTCTACGCACTTCAAAGGTCCTTGTGAATGAAAACCCCGGATTGACTGGAAATCTTCTTGTTGAGCGGTTAGTCGGAGCTCATGTCGAACTTATCTCAAAAGAAGAGTATGCAAAAATTGGAAGCGTGGCACTAACAGATTTTCTAAAGTCAAAATTAGTTGCTGAAGGAAGAAAGCCATATGTTATTCCTGTTGGAGGTTCAAATTCCCTTGGAACTTGGGGCTACATCGAAGCAATTAGGGAGCTTGAACAGCAACTGGATTCCGGAAATGGCAAGATTAAATTTGATGATATTGTAGTAGCTTGTGGCAGTGGGGGCACAGTCGCTGGTTTATCATTGGGATCATGGTTGAGCACATTGAAGACAAAAATTCGTGCGTTCTCTGTTTGTGATGATCCTGATTACTTTTACGAGTTTGTTCAAGGTTTACTTGATGGGCTTCATGCTGGTGTCGACTCACGCGATATAGTTGAAATACAAAATGCAAAGGGTCTTGGCTATGCAATCAACACGCCTGATGAGCTTAATTTCGTAAAAGAAGTTGCAGAATCAACCGGCGTTGTTCTTGATCCAGTATACAGCGGGAAAGCAGCTTATGGTATGATGAAAGACATGGCAGAAAATCCAAAGAAGTGGGAAGGCAGGAAGATTCTGTTCATTCACACAGGAGGGTTGTTGGGACTATATGACAAAGCTGATCAGATCAATTCAACGCTGGGGAAATGGCATCGTTTGGACGTTAATGAGACTGTGCCACGAATTGATGGAGTTGGGAAAATGTTCTAGAACAACTCGGATCAAAAAGTGTAGCCACTATTGAAAGACGGGTGCTTTAAGATTTCTTATCAGACATTTTAATTATTAGGGGTTCAAATATATTGTTATGGATTATTGATAATCCAATAATATATACCTTGTCTTTATTTTGTATTTGCTAGACGTATCATGAATGCCAATTCGTACCTTATTAGTTGAATAAGGCACCGATTGCTATTTTGAATATCGATAGTTCCGTATGTAAATTGTTATTGAAAAAAACCTTGAAGCAAGGAAGTGTATAGTTGGTATTTTCCTTTTGTGTTTTCTTTTTAAAGACAACATAATTTTCAGGG

Coding sequence (CDS)

ATGTTGAGCGGTGGCAAGTTCCAACTGTGTCTACTCACCGCAGCACCATCCAACTTGGCCTCATCGTCATCGTCGTCGTCATCGCTCTTCTCAACTTCTCTAATTCATCCAAGAAGGTTCGACAACATCAACAAGCGTTTCTCCTTTCCTCATCCATCAACTCCGAGTTTAGATTGCATTCGTTGCTGTATTTCACAATCTCAGTCCATGGAGATCAACACTGAAAGCAAGCAACACGACGAGGTCCCTAACAGCCTCAACTTCCTCTCCGTCAGACCTTACGTCCCTCCGTCCTGGGCCTCACACCTTAATCCAATCCCCACCCACCTCTCTTCTCTTGCCCGTCTTCCCACTCCCATTCACAAGTGGAACCTTCCTAATCTGCCCAACAACACAGAGGTCTGGCTCAAGCGTGATGATCTTTCAGGAATGCAATTGAGTGGAAATAAAGTCAGAAAATTAGAATTCTTGATAGCAGATGCTTTGCAGCAGGGTGCTGACTGTATTATAACTATAGGAGGCATCCAAAGCAATCACTGTCGTGCTACTGCTGTGGCTGCAAAGTATTTCAATCTTGACACATATCTAATTCTACGCACTTCAAAGGTCCTTGTGAATGAAAACCCCGGATTGACTGGAAATCTTCTTGTTGAGCGGTTAGTCGGAGCTCATGTCGAACTTATCTCAAAAGAAGAGTATGCAAAAATTGGAAGCGTGGCACTAACAGATTTTCTAAAGTCAAAATTAGTTGCTGAAGGAAGAAAGCCATATGTTATTCCTGTTGGAGGTTCAAATTCCCTTGGAACTTGGGGCTACATCGAAGCAATTAGGGAGCTTGAACAGCAACTGGATTCCGGAAATGGCAAGATTAAATTTGATGATATTGTAGTAGCTTGTGGCAGTGGGGGCACAGTCGCTGGTTTATCATTGGGATCATGGTTGAGCACATTGAAGACAAAAATTCGTGCGTTCTCTGTTTGTGATGATCCTGATTACTTTTACGAGTTTGTTCAAGGTTTACTTGATGGGCTTCATGCTGGTGTCGACTCACGCGATATAGTTGAAATACAAAATGCAAAGGGTCTTGGCTATGCAATCAACACGCCTGATGAGCTTAATTTCGTAAAAGAAGTTGCAGAATCAACCGGCGTTGTTCTTGATCCAGTATACAGCGGGAAAGCAGCTTATGGTATGATGAAAGACATGGCAGAAAATCCAAAGAAGTGGGAAGGCAGGAAGATTCTGTTCATTCACACAGGAGGGTTGTTGGGACTATATGACAAAGCTGATCAGATCAATTCAACGCTGGGGAAATGGCATCGTTTGGACGTTAATGAGACTGTGCCACGAATTGATGGAGTTGGGAAAATGTTCTAG

Protein sequence

MLSGGKFQLCLLTAAPSNLASSSSSSSSLFSTSLIHPRRFDNINKRFSFPHPSTPSLDCIRCCISQSQSMEINTESKQHDEVPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPIHKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHCRATAVAAKYFNLDTYLILRTSKVLVNENPGLTGNLLVERLVGAHVELISKEEYAKIGSVALTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVACGSGGTVAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAKGLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTGGLLGLYDKADQINSTLGKWHRLDVNETVPRIDGVGKMF*
Homology
BLAST of CSPI01G05150 vs. ExPASy Swiss-Prot
Match: Q6ZHE5 (Putative D-cysteine desulfhydrase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0773300 PE=2 SV=2)

HSP 1 Score: 600.5 bits (1547), Expect = 1.6e-170
Identity = 277/372 (74.46%), Postives = 324/372 (87.10%), Query Frame = 0

Query: 87  NFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPIHKWNLPNLPNNTEVWLKRDDLSGMQL 146
           +FLS RPY PPSWASHL+P P+   SL   PTPIHKWNLPNLPN TEVW+KRDD+SGMQL
Sbjct: 56  SFLSKRPYAPPSWASHLSPAPSQTFSLGHFPTPIHKWNLPNLPNGTEVWIKRDDISGMQL 115

Query: 147 SGNKVRKLEFLIADALQQGADCIITIGGIQSNHCRATAVAAKYFNLDTYLILRTSKVLVN 206
           SGNKVRKLEFL+ADA+ QGADC+IT+GGIQSNHCRATAVAAKY NLD YLILRTSK+LV+
Sbjct: 116 SGNKVRKLEFLMADAVAQGADCVITVGGIQSNHCRATAVAAKYINLDCYLILRTSKLLVD 175

Query: 207 ENPGLTGNLLVERLVGAHVELISKEEYAKIGSVALTDFLKSKLVAEGRKPYVIPVGGSNS 266
           ++PGL GNLLVERLVGAH++L+SKEEY KIGSVAL D LK KL+ EGRKPYVIPVGGSNS
Sbjct: 176 KDPGLVGNLLVERLVGAHIDLVSKEEYGKIGSVALADLLKKKLLEEGRKPYVIPVGGSNS 235

Query: 267 LGTWGYIEAIRELEQQLDSGNGKIKFDDIVVACGSGGTVAGLSLGSWLSTLKTKIRAFSV 326
           LGTWGYIEAIRE+E Q+   +G ++FDDIVVACGSGGT+AGL+LGS LS+LK K+ AFSV
Sbjct: 236 LGTWGYIEAIREIEHQIQI-SGDVQFDDIVVACGSGGTIAGLALGSKLSSLKAKVHAFSV 295

Query: 327 CDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAKGLGYAINTPDELNFVKEVAESTGVVL 386
           CDDP YF+ +VQ L+DGLH+ + S D+V I+NAKGLGYA+NT +EL FVK++A +TG+VL
Sbjct: 296 CDDPGYFHSYVQDLIDGLHSDLRSHDLVNIENAKGLGYAMNTAEELKFVKDIATATGIVL 355

Query: 387 DPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTGGLLGLYDKADQINSTLGKWHRLDVNE 446
           DPVYSGKAAYGM+KDM  NP KWEGRKILF+HTGGLLGLYDK D+++S  G W R+D+ E
Sbjct: 356 DPVYSGKAAYGMLKDMGANPAKWEGRKILFVHTGGLLGLYDKVDELSSLSGSWRRMDLEE 415

Query: 447 TVPRIDGVGKMF 459
           +VPR DG GKMF
Sbjct: 416 SVPRKDGTGKMF 426

BLAST of CSPI01G05150 vs. ExPASy Swiss-Prot
Match: F4HYF3 (Bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DCD PE=1 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 5.1e-169
Identity = 278/389 (71.47%), Postives = 330/389 (84.83%), Query Frame = 0

Query: 70  MEINTESKQHDEVPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPIHKWNLPNLP 129
           +E+   S     VP+  +FL+ +PY PPSWASHL P+P+H  SLA LPTPIH+WNLP LP
Sbjct: 13  LELARRSMSATSVPSMADFLTKKPYSPPSWASHLRPLPSHTFSLAHLPTPIHRWNLPGLP 72

Query: 130 NNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHCRATAVAAKY 189
           N TE+W+KRDD +GM+LSGNKVRKLEFL+A+A+ Q AD +ITIGGIQSNHCRATA A+ Y
Sbjct: 73  NGTELWIKRDDFTGMELSGNKVRKLEFLMAEAVDQHADTVITIGGIQSNHCRATATASNY 132

Query: 190 FNLDTYLILRTSKVLVNENPGLTGNLLVERLVGAHVELISKEEYAKIGSVALTDFLKSKL 249
            NL+++LILRTSK+L +E+PGL GNLLVERLVGA+V LISKEEY+ IGS ALT+ LK KL
Sbjct: 133 LNLNSHLILRTSKLLADEDPGLVGNLLVERLVGANVHLISKEEYSSIGSEALTNALKEKL 192

Query: 250 VAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVACGSGGTVAGLS 309
             EG+KPYVIPVGGSNSLGTWGYIEA RE+E+QL+     +KFDDIVVACGSGGT+AG+S
Sbjct: 193 EKEGKKPYVIPVGGSNSLGTWGYIEAAREIEEQLNYRPDDLKFDDIVVACGSGGTIAGIS 252

Query: 310 LGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAKGLGYAINTP 369
           LGSWL  LK K+ AFSVCDDPDYFY+FVQGLLDGLHAGV+SRDIV I NAKG GYA+NT 
Sbjct: 253 LGSWLGALKAKVHAFSVCDDPDYFYDFVQGLLDGLHAGVNSRDIVNIHNAKGKGYAMNTS 312

Query: 370 DELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTGGLLGLYDKA 429
           +EL FVK+VA STGV+LDPVYSGKAAYG++ ++ ++PK WEGRKILFIHTGGLLGLYDK 
Sbjct: 313 EELEFVKKVASSTGVILDPVYSGKAAYGLINEITKDPKCWEGRKILFIHTGGLLGLYDKV 372

Query: 430 DQINSTLGKWHRLDVNETVPRIDGVGKMF 459
           DQ+ S +G W R+DV+E+VPR DGVGKMF
Sbjct: 373 DQMASLMGNWSRMDVSESVPRKDGVGKMF 401

BLAST of CSPI01G05150 vs. ExPASy Swiss-Prot
Match: Q9WY68 (Putative 1-aminocyclopropane-1-carboxylate deaminase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=TM_0225 PE=3 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 2.7e-53
Identity = 125/324 (38.58%), Postives = 191/324 (58.95%), Query Frame = 0

Query: 113 LARLPTPIHKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITI 172
           L+  PTP+      +      +++KRDDL+ +  SGNK+RKLE+L+ +AL++GA  + T 
Sbjct: 5   LSLKPTPVQFLKRLSEKYGFNIYVKRDDLTELVGSGNKIRKLEYLLWEALKKGATTVFTC 64

Query: 173 GGIQSNHCRATAVAAKYFNLDTYLILRTSKVLVNENPGLTGNLLVERLVGAHVELISKEE 232
           GG+QSNH RATA  ++ + L   L LR  + ++N      GNLL++ L+GA +  +S EE
Sbjct: 65  GGLQSNHARATAYVSRRYGLKPVLFLRKGEKVLN------GNLLLDILLGAEIVEVSPEE 124

Query: 233 YAKIGSVALTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKF 292
           Y +I  +   D  K     +G K YVIP GGSNSLG +GY  A+ E++ QL+  +    F
Sbjct: 125 YERIDEI--FDVHKKMREKKGEKVYVIPEGGSNSLGAFGYFNAVLEMKDQLNLES----F 184

Query: 293 DDIVVACGSGGTVAGLSLG-SWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLH---AGV 352
           D IV A GSGGT+AGLS G S+L      +       + DYF   V+ ++ G+      V
Sbjct: 185 DAIVCAVGSGGTIAGLSAGISFLEYHVPVVGVNVTTKNSDYFVGKVKRIISGMEEYGLRV 244

Query: 353 DSRDIVEIQNAKGLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKK 412
           +      + + +G GYAI + +++  +KEVA   G++LDPVY+ KA  GM+ +M  N +K
Sbjct: 245 NETVFEVVDDYRGPGYAIPSSEDVEILKEVASIEGIILDPVYTAKAFRGMI-EMFRNSEK 304

Query: 413 WEGRKILFIHTGGLLGLYDKADQI 433
                +LFIHTGG+ GL+ ++ ++
Sbjct: 305 ----NVLFIHTGGIFGLFAQSRRL 311

BLAST of CSPI01G05150 vs. ExPASy Swiss-Prot
Match: O57809 (Putative 1-aminocyclopropane-1-carboxylate deaminase OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) OX=70601 GN=PH0054 PE=1 SV=2)

HSP 1 Score: 207.2 bits (526), Expect = 3.9e-52
Identity = 132/344 (38.37%), Postives = 191/344 (55.52%), Query Frame = 0

Query: 107 PTHLSSLARLP-TPIHKWNLP--NLPN-----NTEVWLKRDDLSGMQLSGNKVRKLEFLI 166
           P   + LA+ P   +  W  P   LPN       +V++KRDDL+G+ + GNK+RKLE+L+
Sbjct: 3   PKIFALLAKFPRVELIPWETPIQYLPNISREIGADVYIKRDDLTGLGIGGNKIRKLEYLL 62

Query: 167 ADALQQGADCIITIGGIQSNHCRATAVAAKYFNLDTYLILRTSKVLVNENPGLTGNLLVE 226
            DAL +GAD +IT+G + SNH   T +AAK   LD  L+LR  +        L GN L++
Sbjct: 63  GDALSKGADVVITVGAVHSNHAFVTGLAAKKLGLDAILVLRGKE-------ELKGNYLLD 122

Query: 227 RLVGAHVELISKEEYAKIGSVALTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRE 286
           +++G    +   ++  ++   A  + +  +L  EGRKPYVIP GG++ +GT GY+ A+ E
Sbjct: 123 KIMGIETRVYDAKDSFELMKYA--EEIAEELKREGRKPYVIPPGGASPIGTLGYVRAVGE 182

Query: 287 LEQQLDSGNGKIKFDDIVVACGSGGTVAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQ 346
           +  Q      ++KFD IVVA GSGGT+AGLSLG  LS L   IR   +      F E + 
Sbjct: 183 IATQ-----SEVKFDSIVVAAGSGGTLAGLSLG--LSILNEDIRPVGIA--VGRFGEVMT 242

Query: 347 GLLDGL------HAGVDSRDIVEIQNAKGLGYAINTPDELNFVKEVAESTGVVLDPVYSG 406
             LD L        GV      E+ +     Y   T +    +++V    G++LDPVY+G
Sbjct: 243 SKLDNLIKEAAELLGVKVEVRPELYDYSFGEYGKITGEVAQIIRKVGTREGIILDPVYTG 302

Query: 407 KAAYGMMKDMAENPKKWEGRKILFIHTGGLLGLYDKADQINSTL 437
           KA YG++ D+A   K   G KILFIHTGG+ G +   D++ S L
Sbjct: 303 KAFYGLV-DLAR--KGELGEKILFIHTGGISGTFHYGDKLLSLL 325

BLAST of CSPI01G05150 vs. ExPASy Swiss-Prot
Match: Q8U4R3 (Putative 1-aminocyclopropane-1-carboxylate deaminase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=PF0010 PE=3 SV=2)

HSP 1 Score: 193.0 bits (489), Expect = 7.6e-48
Identity = 124/326 (38.04%), Postives = 188/326 (57.67%), Query Frame = 0

Query: 118 TPIHKWNLPNLPN--NTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGI 177
           TPI    LPN+      ++++KRDDL+G+ + GNK+RKLE+L+ DA+ + AD IIT+G +
Sbjct: 22  TPIQ--YLPNISKLVGADIYVKRDDLTGLGIGGNKIRKLEYLLGDAIIRKADVIITVGAV 81

Query: 178 QSNHCRATAVAAKYFNLDTYLILRTSKVLVNENPGLTGNLLVERLVGAHVELISKEEYAK 237
            SNH   T +AAK    D  L+LR  +        L GN L+++++G    +   ++  +
Sbjct: 82  HSNHAFVTGLAAKKLGFDVVLVLRGKE-------ELRGNYLLDKIMGIETRVYEAKDSFE 141

Query: 238 IGSVALTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNG-KIKFDD 297
           +   A  + +  +L  +GRKPY+IPVGG++ +GT GY+ A  E+ +Q   GN   + FD 
Sbjct: 142 LMKYA--EEVAKELEEKGRKPYIIPVGGASPVGTLGYVRASGEIAEQ---GNRIGVNFDS 201

Query: 298 IVVACGSGGTVAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGL--LDGLHAGVDSRD 357
           IVVA GSGGT+AGLS+G  +   +T+    +V    +     V+ L    G   GV +  
Sbjct: 202 IVVATGSGGTLAGLSVGLAILRKETRAIGMAVGKFGETMVNKVEELAKATGEFIGVKNLK 261

Query: 358 I-VEIQNAKGLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWE- 417
           + +E+ +     Y   T +    ++ V    GV+LDPVY+GKA YG++ D+A   KK E 
Sbjct: 262 LKIELYDYSFGEYGKITREVAETIRLVGTKEGVILDPVYTGKAFYGLL-DLA---KKGEL 321

Query: 418 GRKILFIHTGGLLGLYDKADQINSTL 437
           G KILFIHTGG+ G +   D+I S L
Sbjct: 322 GEKILFIHTGGISGTFHYGDKILSFL 329

BLAST of CSPI01G05150 vs. ExPASy TrEMBL
Match: A0A1S3CSU2 (bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103504538 PE=3 SV=1)

HSP 1 Score: 897.1 bits (2317), Expect = 3.0e-257
Identity = 443/458 (96.72%), Postives = 452/458 (98.69%), Query Frame = 0

Query: 1   MLSGGKFQLCLLTAAPSNLASSSSSSSSLFSTSLIHPRRFDNINKRFSFPHPSTPSLDCI 60
           MLS GKFQLCLL+AA SN  S+SSSSS+LFSTSLIHPRRFDN+NKRFSFPHPSTPSLDCI
Sbjct: 1   MLSSGKFQLCLLSAASSN--SASSSSSALFSTSLIHPRRFDNLNKRFSFPHPSTPSLDCI 60

Query: 61  RCCISQSQSMEINTESKQHDEVPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPI 120
           RC ISQSQ+MEI TESKQHDE+PNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPI
Sbjct: 61  RCSISQSQAMEIKTESKQHDELPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPI 120

Query: 121 HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC 180
           HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC
Sbjct: 121 HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC 180

Query: 181 RATAVAAKYFNLDTYLILRTSKVLVNENPGLTGNLLVERLVGAHVELISKEEYAKIGSVA 240
           RATAVAAKYFNLDTYLILRTSKVLVNE+PGLTGNLLVERLVGAHVELISKEEYAKIGSVA
Sbjct: 181 RATAVAAKYFNLDTYLILRTSKVLVNEDPGLTGNLLVERLVGAHVELISKEEYAKIGSVA 240

Query: 241 LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVACG 300
           LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVACG
Sbjct: 241 LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVACG 300

Query: 301 SGGTVAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAK 360
           SGGTVAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAK
Sbjct: 301 SGGTVAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAK 360

Query: 361 GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTG 420
           GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTG
Sbjct: 361 GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTG 420

Query: 421 GLLGLYDKADQINSTLGKWHRLDVNETVPRIDGVGKMF 459
           GLLGLYDKADQI+STLGKWHRLDVNE+VPRIDGVGKMF
Sbjct: 421 GLLGLYDKADQISSTLGKWHRLDVNESVPRIDGVGKMF 456

BLAST of CSPI01G05150 vs. ExPASy TrEMBL
Match: A0A5A7T3I8 (Bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold112G00370 PE=3 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 7.2e-251
Identity = 436/458 (95.20%), Postives = 445/458 (97.16%), Query Frame = 0

Query: 1   MLSGGKFQLCLLTAAPSNLASSSSSSSSLFSTSLIHPRRFDNINKRFSFPHPSTPSLDCI 60
           MLS GKFQLCLL+AA SN  S+SSSSS+LFSTSLIHPRRFDN+NKRFSFPHPSTPSLDCI
Sbjct: 1   MLSSGKFQLCLLSAASSN--SASSSSSALFSTSLIHPRRFDNVNKRFSFPHPSTPSLDCI 60

Query: 61  RCCISQSQSMEINTESKQHDEVPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPI 120
           RC ISQSQ+MEI TESKQHDE+PNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPI
Sbjct: 61  RCSISQSQAMEIKTESKQHDELPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPI 120

Query: 121 HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC 180
           HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC
Sbjct: 121 HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC 180

Query: 181 RATAVAAKYFNLDTYLILRTSKVLVNENPGLTGNLLVERLVGAHVELISKEEYAKIGSVA 240
           RATAVAAKYFNLDTYLILRTSKVLVNE+PGLTGNLLVERLVGAHVELISKEEYAKIGSVA
Sbjct: 181 RATAVAAKYFNLDTYLILRTSKVLVNEDPGLTGNLLVERLVGAHVELISKEEYAKIGSVA 240

Query: 241 LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVACG 300
           LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVA  
Sbjct: 241 LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVA-- 300

Query: 301 SGGTVAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAK 360
                AGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAK
Sbjct: 301 ----FAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAK 360

Query: 361 GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTG 420
           GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTG
Sbjct: 361 GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTG 420

Query: 421 GLLGLYDKADQINSTLGKWHRLDVNETVPRIDGVGKMF 459
           GLLGLYDKADQI+STLGKWHRLDVNE+VPRIDGVGKMF
Sbjct: 421 GLLGLYDKADQISSTLGKWHRLDVNESVPRIDGVGKMF 450

BLAST of CSPI01G05150 vs. ExPASy TrEMBL
Match: A0A6J1GBY4 (bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminase, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111452580 PE=3 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 1.7e-223
Identity = 389/460 (84.57%), Postives = 420/460 (91.30%), Query Frame = 0

Query: 1   MLSGGKFQLCLLTAAPSNLASSSSSSSSLFSTSLIHPRRFDNINKRFSFPH--PSTPSLD 60
           MLS GKFQ+ LLTA   +  ++SSSSS+ FS S+IH  R  + N+RFS     P    L 
Sbjct: 1   MLSSGKFQVYLLTA--PSFKAASSSSSTFFSASVIHSGRLRSRNRRFSSSSSPPPNSGLH 60

Query: 61  CIRCCISQSQSMEINTESKQHDEVPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPT 120
            I C ISQSQ+ME+N ESK++D+V +SLNF SV+PYVPPSWASHLNPIPTH SSL  LPT
Sbjct: 61  SIGCSISQSQAMEVNPESKKNDKVLDSLNFFSVKPYVPPSWASHLNPIPTHFSSLGHLPT 120

Query: 121 PIHKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSN 180
           PIHKWNLPNLP NTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSN
Sbjct: 121 PIHKWNLPNLPANTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSN 180

Query: 181 HCRATAVAAKYFNLDTYLILRTSKVLVNENPGLTGNLLVERLVGAHVELISKEEYAKIGS 240
           HCRATAVAAKYFNLDTYLILRTSKVLVN++PGLTGNLLVERLVGAHVELISKEEYAKIGS
Sbjct: 181 HCRATAVAAKYFNLDTYLILRTSKVLVNQDPGLTGNLLVERLVGAHVELISKEEYAKIGS 240

Query: 241 VALTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVA 300
           VALTD+LKSKL+AEGRKPYVIPVGGSNSLGTWGYIEA+RE+EQQLD+ N KIKFDDIVVA
Sbjct: 241 VALTDYLKSKLIAEGRKPYVIPVGGSNSLGTWGYIEAVREIEQQLDTVNDKIKFDDIVVA 300

Query: 301 CGSGGTVAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQN 360
           CGSGGTVAGLSLGSWLSTLK KIRA+SVCDDPDYFY+FVQGLLDGLHAGVDSRDIVE QN
Sbjct: 301 CGSGGTVAGLSLGSWLSTLKAKIRAYSVCDDPDYFYDFVQGLLDGLHAGVDSRDIVEFQN 360

Query: 361 AKGLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIH 420
           AKGLGYA+NTP+ELNFV+EVAESTGVVLDPVYSGKAAYGMMKDM+ENPKKWEGRKILFIH
Sbjct: 361 AKGLGYAMNTPEELNFVREVAESTGVVLDPVYSGKAAYGMMKDMSENPKKWEGRKILFIH 420

Query: 421 TGGLLGLYDKADQINSTLGKWHRLDVNETVPRIDGVGKMF 459
           TGGLLGLYDKADQ+ S LGKWHR+DVNE+VPRIDGVGKMF
Sbjct: 421 TGGLLGLYDKADQMGSRLGKWHRMDVNESVPRIDGVGKMF 458

BLAST of CSPI01G05150 vs. ExPASy TrEMBL
Match: A0A6J1K967 (bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminase, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111492816 PE=3 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 4.9e-223
Identity = 388/459 (84.53%), Postives = 419/459 (91.29%), Query Frame = 0

Query: 1   MLSGGKFQLCLLTAAPSNLASSSSSSSSLFSTSLIHPRRFDNINKRFSFPHPSTPS-LDC 60
           MLS GKF + LLTA   +  ++SSSSS+ FS S+IHP RF + N+RFS   P   S L  
Sbjct: 1   MLSSGKFPVYLLTA--PSFKAASSSSSTFFSASVIHPARFRSRNRRFSSSSPPPNSGLHS 60

Query: 61  IRCCISQSQSMEINTESKQHDEVPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTP 120
           I C ISQSQ+ME+N ESK++D+V +SLNF SVRPYVPPSWASHL PIPTH SSL  LPTP
Sbjct: 61  IGCSISQSQAMEVNPESKKNDKVLDSLNFFSVRPYVPPSWASHLTPIPTHFSSLGHLPTP 120

Query: 121 IHKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNH 180
           IHKWNLPNLP NTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNH
Sbjct: 121 IHKWNLPNLPTNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNH 180

Query: 181 CRATAVAAKYFNLDTYLILRTSKVLVNENPGLTGNLLVERLVGAHVELISKEEYAKIGSV 240
           CRATAVAAKYFNLDTYLILRTSKVLVN++PGLTGNLLVERLVGAHVELISKEEYAKIGSV
Sbjct: 181 CRATAVAAKYFNLDTYLILRTSKVLVNQDPGLTGNLLVERLVGAHVELISKEEYAKIGSV 240

Query: 241 ALTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVAC 300
           ALTD+LKSKL+AEGRKPYVIPVGGSNSLGTWGYIEA+RE+EQQLD+ N KIKFDDIVVAC
Sbjct: 241 ALTDYLKSKLIAEGRKPYVIPVGGSNSLGTWGYIEAVREIEQQLDTVNDKIKFDDIVVAC 300

Query: 301 GSGGTVAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNA 360
           GSGGTVAGLSLGSWLS LK KIRA+SVCDDPDYFY+FVQGLLDGLHAGVDSRD+VE QNA
Sbjct: 301 GSGGTVAGLSLGSWLSKLKAKIRAYSVCDDPDYFYDFVQGLLDGLHAGVDSRDVVEFQNA 360

Query: 361 KGLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHT 420
           KGLGYA+NTP+ELNFV+EVAESTGVVLDPVYSGKAAY MMKDM+ENPKKWEGRKILFIHT
Sbjct: 361 KGLGYAMNTPEELNFVREVAESTGVVLDPVYSGKAAYEMMKDMSENPKKWEGRKILFIHT 420

Query: 421 GGLLGLYDKADQINSTLGKWHRLDVNETVPRIDGVGKMF 459
           GGLLGLYDKADQ+ S LGKWHR+DVNE+VPRIDGVGKMF
Sbjct: 421 GGLLGLYDKADQMGSMLGKWHRMDVNESVPRIDGVGKMF 457

BLAST of CSPI01G05150 vs. ExPASy TrEMBL
Match: A0A0A0LVC4 (PALP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G031770 PE=3 SV=1)

HSP 1 Score: 780.8 bits (2015), Expect = 3.2e-222
Identity = 399/458 (87.12%), Postives = 406/458 (88.65%), Query Frame = 0

Query: 1   MLSGGKFQLCLLTAAPSNLASSSSSSSSLFSTSLIHPRRFDNINKRFSFPHPSTPSLDCI 60
           MLSGGKFQLCLLTAAPSNLA    SSSSLFSTSLIHPRRFDNINKRFSFPHPSTPSLDCI
Sbjct: 1   MLSGGKFQLCLLTAAPSNLA----SSSSLFSTSLIHPRRFDNINKRFSFPHPSTPSLDCI 60

Query: 61  RCCISQSQSMEINTESKQHDEVPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPI 120
           RCCISQSQSMEINTESKQHDEVPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPI
Sbjct: 61  RCCISQSQSMEINTESKQHDEVPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPI 120

Query: 121 HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC 180
           HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC
Sbjct: 121 HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC 180

Query: 181 RATAVAAKYFNLDTYLILRTSKVLVNENPGLTGNLLVERLVGAHVELISKEEYAKIGSVA 240
           RATAVAAKYFNLDTYLILRTSKVLVNE+PGLTGNLLVERLVGAHVELISKEEYAKIGSVA
Sbjct: 181 RATAVAAKYFNLDTYLILRTSKVLVNEDPGLTGNLLVERLVGAHVELISKEEYAKIGSVA 240

Query: 241 LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVACG 300
           LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVACG
Sbjct: 241 LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVACG 300

Query: 301 SGGTVAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAK 360
              ++     G W +     +    +C D +                           AK
Sbjct: 301 RTFSMK----GCWFA----HVWQHHLCCDVE---------------------------AK 360

Query: 361 GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTG 420
           GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTG
Sbjct: 361 GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTG 419

Query: 421 GLLGLYDKADQINSTLGKWHRLDVNETVPRIDGVGKMF 459
           GLLGLYDKADQINSTLGKWHRLDVNETVPRIDGVGKMF
Sbjct: 421 GLLGLYDKADQINSTLGKWHRLDVNETVPRIDGVGKMF 419

BLAST of CSPI01G05150 vs. NCBI nr
Match: XP_004137385.1 (bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminase, mitochondrial [Cucumis sativus] >KAE8652497.1 hypothetical protein Csa_013952 [Cucumis sativus])

HSP 1 Score: 916.4 bits (2367), Expect = 9.9e-263
Identity = 453/458 (98.91%), Postives = 454/458 (99.13%), Query Frame = 0

Query: 1   MLSGGKFQLCLLTAAPSNLASSSSSSSSLFSTSLIHPRRFDNINKRFSFPHPSTPSLDCI 60
           MLSGGKFQLCLLTAAPSNLA    SSSSLFSTSLIHPRRFDNINKRFSFPHPSTPSLDCI
Sbjct: 1   MLSGGKFQLCLLTAAPSNLA----SSSSLFSTSLIHPRRFDNINKRFSFPHPSTPSLDCI 60

Query: 61  RCCISQSQSMEINTESKQHDEVPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPI 120
           RCCISQSQSMEINTESKQHDEVPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPI
Sbjct: 61  RCCISQSQSMEINTESKQHDEVPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPI 120

Query: 121 HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC 180
           HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC
Sbjct: 121 HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC 180

Query: 181 RATAVAAKYFNLDTYLILRTSKVLVNENPGLTGNLLVERLVGAHVELISKEEYAKIGSVA 240
           RATAVAAKYFNLDTYLILRTSKVLVNE+PGLTGNLLVERLVGAHVELISKEEYAKIGSVA
Sbjct: 181 RATAVAAKYFNLDTYLILRTSKVLVNEDPGLTGNLLVERLVGAHVELISKEEYAKIGSVA 240

Query: 241 LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVACG 300
           LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVACG
Sbjct: 241 LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVACG 300

Query: 301 SGGTVAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAK 360
           SGGTVAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAK
Sbjct: 301 SGGTVAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAK 360

Query: 361 GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTG 420
           GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTG
Sbjct: 361 GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTG 420

Query: 421 GLLGLYDKADQINSTLGKWHRLDVNETVPRIDGVGKMF 459
           GLLGLYDKADQINSTLGKWHRLDVNETVPRIDGVGKMF
Sbjct: 421 GLLGLYDKADQINSTLGKWHRLDVNETVPRIDGVGKMF 454

BLAST of CSPI01G05150 vs. NCBI nr
Match: XP_008467141.1 (PREDICTED: bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminase, mitochondrial [Cucumis melo])

HSP 1 Score: 897.1 bits (2317), Expect = 6.2e-257
Identity = 443/458 (96.72%), Postives = 452/458 (98.69%), Query Frame = 0

Query: 1   MLSGGKFQLCLLTAAPSNLASSSSSSSSLFSTSLIHPRRFDNINKRFSFPHPSTPSLDCI 60
           MLS GKFQLCLL+AA SN  S+SSSSS+LFSTSLIHPRRFDN+NKRFSFPHPSTPSLDCI
Sbjct: 1   MLSSGKFQLCLLSAASSN--SASSSSSALFSTSLIHPRRFDNLNKRFSFPHPSTPSLDCI 60

Query: 61  RCCISQSQSMEINTESKQHDEVPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPI 120
           RC ISQSQ+MEI TESKQHDE+PNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPI
Sbjct: 61  RCSISQSQAMEIKTESKQHDELPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPI 120

Query: 121 HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC 180
           HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC
Sbjct: 121 HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC 180

Query: 181 RATAVAAKYFNLDTYLILRTSKVLVNENPGLTGNLLVERLVGAHVELISKEEYAKIGSVA 240
           RATAVAAKYFNLDTYLILRTSKVLVNE+PGLTGNLLVERLVGAHVELISKEEYAKIGSVA
Sbjct: 181 RATAVAAKYFNLDTYLILRTSKVLVNEDPGLTGNLLVERLVGAHVELISKEEYAKIGSVA 240

Query: 241 LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVACG 300
           LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVACG
Sbjct: 241 LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVACG 300

Query: 301 SGGTVAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAK 360
           SGGTVAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAK
Sbjct: 301 SGGTVAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAK 360

Query: 361 GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTG 420
           GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTG
Sbjct: 361 GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTG 420

Query: 421 GLLGLYDKADQINSTLGKWHRLDVNETVPRIDGVGKMF 459
           GLLGLYDKADQI+STLGKWHRLDVNE+VPRIDGVGKMF
Sbjct: 421 GLLGLYDKADQISSTLGKWHRLDVNESVPRIDGVGKMF 456

BLAST of CSPI01G05150 vs. NCBI nr
Match: KAA0036045.1 (bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminase [Cucumis melo var. makuwa])

HSP 1 Score: 875.9 bits (2262), Expect = 1.5e-250
Identity = 436/458 (95.20%), Postives = 445/458 (97.16%), Query Frame = 0

Query: 1   MLSGGKFQLCLLTAAPSNLASSSSSSSSLFSTSLIHPRRFDNINKRFSFPHPSTPSLDCI 60
           MLS GKFQLCLL+AA SN  S+SSSSS+LFSTSLIHPRRFDN+NKRFSFPHPSTPSLDCI
Sbjct: 1   MLSSGKFQLCLLSAASSN--SASSSSSALFSTSLIHPRRFDNVNKRFSFPHPSTPSLDCI 60

Query: 61  RCCISQSQSMEINTESKQHDEVPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPI 120
           RC ISQSQ+MEI TESKQHDE+PNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPI
Sbjct: 61  RCSISQSQAMEIKTESKQHDELPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPI 120

Query: 121 HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC 180
           HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC
Sbjct: 121 HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC 180

Query: 181 RATAVAAKYFNLDTYLILRTSKVLVNENPGLTGNLLVERLVGAHVELISKEEYAKIGSVA 240
           RATAVAAKYFNLDTYLILRTSKVLVNE+PGLTGNLLVERLVGAHVELISKEEYAKIGSVA
Sbjct: 181 RATAVAAKYFNLDTYLILRTSKVLVNEDPGLTGNLLVERLVGAHVELISKEEYAKIGSVA 240

Query: 241 LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVACG 300
           LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVA  
Sbjct: 241 LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVA-- 300

Query: 301 SGGTVAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAK 360
                AGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAK
Sbjct: 301 ----FAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAK 360

Query: 361 GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTG 420
           GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTG
Sbjct: 361 GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTG 420

Query: 421 GLLGLYDKADQINSTLGKWHRLDVNETVPRIDGVGKMF 459
           GLLGLYDKADQI+STLGKWHRLDVNE+VPRIDGVGKMF
Sbjct: 421 GLLGLYDKADQISSTLGKWHRLDVNESVPRIDGVGKMF 450

BLAST of CSPI01G05150 vs. NCBI nr
Match: XP_038895263.1 (LOW QUALITY PROTEIN: bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminase, mitochondrial [Benincasa hispida])

HSP 1 Score: 852.0 bits (2200), Expect = 2.3e-243
Identity = 421/458 (91.92%), Postives = 440/458 (96.07%), Query Frame = 0

Query: 1   MLSGGKFQLCLLTAAPSNLASSSSSSSSLFSTSLIHPRRFDNINKRFSFPHPSTPSLDCI 60
           MLS GKFQLCLLT+A S+  S+SSSSS+LFST LIHP RF+N+N+RFSF  PS PSLDCI
Sbjct: 1   MLSSGKFQLCLLTSASSS-NSASSSSSALFSTPLIHPGRFNNLNQRFSFSPPSGPSLDCI 60

Query: 61  RCCISQSQSMEINTESKQHDEVPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPI 120
           RC ISQSQ+MEINTESK++DEV NSLNFLSVRPYVPPSWASHLNPIPTH SSLARLPTPI
Sbjct: 61  RCSISQSQAMEINTESKKNDEVRNSLNFLSVRPYVPPSWASHLNPIPTHFSSLARLPTPI 120

Query: 121 HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC 180
           HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC
Sbjct: 121 HKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHC 180

Query: 181 RATAVAAKYFNLDTYLILRTSKVLVNENPGLTGNLLVERLVGAHVELISKEEYAKIGSVA 240
           RATAVAAKYFNLDT+LILRTSKVLVN++PGLTGNLLVERLVGAHVELISKEEYA IGSVA
Sbjct: 181 RATAVAAKYFNLDTFLILRTSKVLVNQDPGLTGNLLVERLVGAHVELISKEEYAIIGSVA 240

Query: 241 LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVACG 300
           LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRE+EQQLDSGN KIKFDDIVVACG
Sbjct: 241 LTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIREIEQQLDSGNDKIKFDDIVVACG 300

Query: 301 SGGTVAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAK 360
           SGGTVAGLSLGSWLS+LK K+RAFSVCDDPDYFY FVQGLLDGLHAG+DSRDIVEIQNAK
Sbjct: 301 SGGTVAGLSLGSWLSSLKAKLRAFSVCDDPDYFYNFVQGLLDGLHAGIDSRDIVEIQNAK 360

Query: 361 GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTG 420
           GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENP KWEGRKILFIHTG
Sbjct: 361 GLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPNKWEGRKILFIHTG 420

Query: 421 GLLGLYDKADQINSTLGKWHRLDVNETVPRIDGVGKMF 459
           GLLGLYDKADQI+STLGKWHRLDVNE+ PRIDGVGKMF
Sbjct: 421 GLLGLYDKADQISSTLGKWHRLDVNESXPRIDGVGKMF 457

BLAST of CSPI01G05150 vs. NCBI nr
Match: XP_023522011.1 (bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminase, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 789.3 bits (2037), Expect = 1.8e-224
Identity = 391/460 (85.00%), Postives = 420/460 (91.30%), Query Frame = 0

Query: 1   MLSGGKFQLCLLTAAPSNLASSSSSSSSLFSTSLIHPRRFDNINKRFSFPH--PSTPSLD 60
           MLS GKFQ+ LLTA  S   S+SS SS+ FS S+IHP RF + N+RFS     P    L 
Sbjct: 1   MLSSGKFQVYLLTATSSK--SASSLSSTFFSASVIHPARFRSRNRRFSSSSSPPPNSGLH 60

Query: 61  CIRCCISQSQSMEINTESKQHDEVPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPT 120
            I C ISQSQ+ME+N ESK++D+V +SLNF SV+PYVPPSWASHLNPIPTH SSL  LPT
Sbjct: 61  SIGCSISQSQAMEVNPESKKNDKVLDSLNFFSVKPYVPPSWASHLNPIPTHFSSLGHLPT 120

Query: 121 PIHKWNLPNLPNNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSN 180
           PIHKWNLPNLP NTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSN
Sbjct: 121 PIHKWNLPNLPANTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSN 180

Query: 181 HCRATAVAAKYFNLDTYLILRTSKVLVNENPGLTGNLLVERLVGAHVELISKEEYAKIGS 240
           HCRATAVAAKYFNLDTYLILRTSKVLVN++PGLTGNLLVERLVGAHVELISKEEYAKIGS
Sbjct: 181 HCRATAVAAKYFNLDTYLILRTSKVLVNQDPGLTGNLLVERLVGAHVELISKEEYAKIGS 240

Query: 241 VALTDFLKSKLVAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVA 300
           VALTD+LKSKL+AEGRKPYVIPVGGSNSLGTWGYIEA+RE+EQQLD+ N KIKFDDIVVA
Sbjct: 241 VALTDYLKSKLIAEGRKPYVIPVGGSNSLGTWGYIEAVREIEQQLDTVNDKIKFDDIVVA 300

Query: 301 CGSGGTVAGLSLGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQN 360
           CGSGGTVAGLSLGSWLSTLK KIRA+SVCDDPDYFY+FVQGLLDGLHA VDSRDIVE QN
Sbjct: 301 CGSGGTVAGLSLGSWLSTLKAKIRAYSVCDDPDYFYDFVQGLLDGLHASVDSRDIVEFQN 360

Query: 361 AKGLGYAINTPDELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIH 420
           AKGLGYA+NTP+ELNFV+EVAESTGVVLDPVYSGKAAYGMMKDM+ENPKKWEGRKILFIH
Sbjct: 361 AKGLGYAMNTPEELNFVREVAESTGVVLDPVYSGKAAYGMMKDMSENPKKWEGRKILFIH 420

Query: 421 TGGLLGLYDKADQINSTLGKWHRLDVNETVPRIDGVGKMF 459
           TGGLLGLYDKADQ+ S LGKWHR+DVNE+VPRIDGVGKMF
Sbjct: 421 TGGLLGLYDKADQMGSMLGKWHRMDVNESVPRIDGVGKMF 458

BLAST of CSPI01G05150 vs. TAIR 10
Match: AT1G48420.1 (D-cysteine desulfhydrase )

HSP 1 Score: 595.5 bits (1534), Expect = 3.6e-170
Identity = 278/389 (71.47%), Postives = 330/389 (84.83%), Query Frame = 0

Query: 70  MEINTESKQHDEVPNSLNFLSVRPYVPPSWASHLNPIPTHLSSLARLPTPIHKWNLPNLP 129
           +E+   S     VP+  +FL+ +PY PPSWASHL P+P+H  SLA LPTPIH+WNLP LP
Sbjct: 13  LELARRSMSATSVPSMADFLTKKPYSPPSWASHLRPLPSHTFSLAHLPTPIHRWNLPGLP 72

Query: 130 NNTEVWLKRDDLSGMQLSGNKVRKLEFLIADALQQGADCIITIGGIQSNHCRATAVAAKY 189
           N TE+W+KRDD +GM+LSGNKVRKLEFL+A+A+ Q AD +ITIGGIQSNHCRATA A+ Y
Sbjct: 73  NGTELWIKRDDFTGMELSGNKVRKLEFLMAEAVDQHADTVITIGGIQSNHCRATATASNY 132

Query: 190 FNLDTYLILRTSKVLVNENPGLTGNLLVERLVGAHVELISKEEYAKIGSVALTDFLKSKL 249
            NL+++LILRTSK+L +E+PGL GNLLVERLVGA+V LISKEEY+ IGS ALT+ LK KL
Sbjct: 133 LNLNSHLILRTSKLLADEDPGLVGNLLVERLVGANVHLISKEEYSSIGSEALTNALKEKL 192

Query: 250 VAEGRKPYVIPVGGSNSLGTWGYIEAIRELEQQLDSGNGKIKFDDIVVACGSGGTVAGLS 309
             EG+KPYVIPVGGSNSLGTWGYIEA RE+E+QL+     +KFDDIVVACGSGGT+AG+S
Sbjct: 193 EKEGKKPYVIPVGGSNSLGTWGYIEAAREIEEQLNYRPDDLKFDDIVVACGSGGTIAGIS 252

Query: 310 LGSWLSTLKTKIRAFSVCDDPDYFYEFVQGLLDGLHAGVDSRDIVEIQNAKGLGYAINTP 369
           LGSWL  LK K+ AFSVCDDPDYFY+FVQGLLDGLHAGV+SRDIV I NAKG GYA+NT 
Sbjct: 253 LGSWLGALKAKVHAFSVCDDPDYFYDFVQGLLDGLHAGVNSRDIVNIHNAKGKGYAMNTS 312

Query: 370 DELNFVKEVAESTGVVLDPVYSGKAAYGMMKDMAENPKKWEGRKILFIHTGGLLGLYDKA 429
           +EL FVK+VA STGV+LDPVYSGKAAYG++ ++ ++PK WEGRKILFIHTGGLLGLYDK 
Sbjct: 313 EELEFVKKVASSTGVILDPVYSGKAAYGLINEITKDPKCWEGRKILFIHTGGLLGLYDKV 372

Query: 430 DQINSTLGKWHRLDVNETVPRIDGVGKMF 459
           DQ+ S +G W R+DV+E+VPR DGVGKMF
Sbjct: 373 DQMASLMGNWSRMDVSESVPRKDGVGKMF 401

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6ZHE51.6e-17074.46Putative D-cysteine desulfhydrase 1, mitochondrial OS=Oryza sativa subsp. japoni... [more]
F4HYF35.1e-16971.47Bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminas... [more]
Q9WY682.7e-5338.58Putative 1-aminocyclopropane-1-carboxylate deaminase OS=Thermotoga maritima (str... [more]
O578093.9e-5238.37Putative 1-aminocyclopropane-1-carboxylate deaminase OS=Pyrococcus horikoshii (s... [more]
Q8U4R37.6e-4838.04Putative 1-aminocyclopropane-1-carboxylate deaminase OS=Pyrococcus furiosus (str... [more]
Match NameE-valueIdentityDescription
A0A1S3CSU23.0e-25796.72bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminas... [more]
A0A5A7T3I87.2e-25195.20Bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminas... [more]
A0A6J1GBY41.7e-22384.57bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminas... [more]
A0A6J1K9674.9e-22384.53bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminas... [more]
A0A0A0LVC43.2e-22287.12PALP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G031770 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_004137385.19.9e-26398.91bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminas... [more]
XP_008467141.16.2e-25796.72PREDICTED: bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxyla... [more]
KAA0036045.11.5e-25095.20bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminas... [more]
XP_038895263.12.3e-24391.92LOW QUALITY PROTEIN: bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1... [more]
XP_023522011.11.8e-22485.00bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminas... [more]
Match NameE-valueIdentityDescription
AT1G48420.13.6e-17071.47D-cysteine desulfhydrase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001926Pyridoxal-phosphate dependent enzymePFAMPF00291PALPcoord: 114..420
e-value: 1.3E-34
score: 120.0
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 149..261
e-value: 2.7E-97
score: 327.4
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 112..423
e-value: 2.7E-97
score: 327.4
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeSUPERFAMILY53686Tryptophan synthase beta subunit-like PLP-dependent enzymescoord: 111..426
IPR005966D-cysteine desulfhydraseTIGRFAMTIGR01275TIGR01275coord: 112..432
e-value: 6.5E-130
score: 431.0
IPR0272781-aminocyclopropane-1-carboxylate deaminase/D-cysteine desulfhydrasePANTHERPTHR437801-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE-RELATEDcoord: 86..448
NoneNo IPR availablePANTHERPTHR43780:SF8SUBFAMILY NOT NAMEDcoord: 86..448

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G05150.1CSPI01G05150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019447 D-cysteine catabolic process
biological_process GO:0009693 ethylene biosynthetic process
biological_process GO:1990170 stress response to cadmium ion
cellular_component GO:0005739 mitochondrion
molecular_function GO:0019148 D-cysteine desulfhydrase activity
molecular_function GO:0003824 catalytic activity