CSPI01G03720 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G03720
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein
Location	Chr1: 2327502 .. 2328691 (+)
RNA-Seq Expression	CSPI01G03720
Synteny	CSPI01G03720

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCTTAATTCGATTATGAAGAAAAAGTTGAATCTCACAGTGAGCTTAGAGTTTGGCAAGAAAAAACAAAAACAAAAACTTAGAAAGTTGAAGTTATTTGCCCTTAACTCATGAGTCTTTCACTCCAAGCATGTTTTGCTTTGGCACTCACAAGTATTGGAGAGGGAAGAACAAGAAGTGTGTGGAATGGCACACAGACAGCATGTTTCAACTCACTTAAACAATGAACTTTCCCTCTATTTCTACACTGTAAAAGCTAAAGAAAGCTTTATTGACTTTGCAACCATGGCATCTCTTCCACATCTCCCCCTTCCTCAACAAGCTATCTCATACCTAAGTCTGCTGTTTCTCCTGCACGTTCTACCGATCACCATATCACAAGGTCCAACCATATCAACTCCCACCATAGCTCAATGCACGACGAGTATTCTTCCATTGGCTTCTTGTGCACCTTTTGTGCAAGGTGTAACACCAACACCACCTATGGGATGCTGTGATAACCTGAAACAACTCTACAACGTTGTCCCTAACTGCCTTTGCCTCTTGCTGAATGGCACTAACTTAAGCTCTTTCCCTATCAACACTACTAGAGCCCTCCAGCTGCCTGATATTTGCAGCCTTCAAGTTAACATCTCCACTTGTTCAGGTAAAACTATAATCCTGTCATCCTAAAAGATGTTATATCTTTTCTACTCTCTACTTCTCTCTACAATCTGATCCCACGGTTTAACATTTATAATTCTTTGAACGGTTCTTTGAAGCTCTCCTTGGGCCACCTAGTCCACCCAGTTCTCATGTTCCTCCTAGAGTACACATCAACTCCACAATTAGCAATGCTACGGTTCTAGGTATACTTCAGATATTCATCCAGACGATATAGTTTTTGGTTCAGTTCTACTTCTAATACAGCCTTCGAATATGTAACAATCTGACTCTACTTTTGTAAATTTCAGCTTCTCCAATGACTCAACCAACACCAAAACCTAGCATTGTGGGTTTAGGATTGGGCAAGATCAGTGCTAGTTTCAAATTAAGGGGAGGAATTCAGCTCACAGTAGTGATAACAATAAGCACTTTTCTGTTTCTAGTAGTACTTTATTGAAATAAATTATCATATGATGTATGGGGTTGCTTCAGCAGCTAGCAGTGATGTTCTTCTTTATTAGACTTCTACTAGATTGTTTTAGTTAA

mRNA sequence

GCCTTAATTCGATTATGAAGAAAAAGTTGAATCTCACAGTGAGCTTAGAGTTTGGCAAGAAAAAACAAAAACAAAAACTTAGAAAGTTGAAGTTATTTGCCCTTAACTCATGAGTCTTTCACTCCAAGCATGTTTTGCTTTGGCACTCACAAGTATTGGAGAGGGAAGAACAAGAAGTGTGTGGAATGGCACACAGACAGCATGTTTCAACTCACTTAAACAATGAACTTTCCCTCTATTTCTACACTGTAAAAGCTAAAGAAAGCTTTATTGACTTTGCAACCATGGCATCTCTTCCACATCTCCCCCTTCCTCAACAAGCTATCTCATACCTAAGTCTGCTGTTTCTCCTGCACGTTCTACCGATCACCATATCACAAGGTCCAACCATATCAACTCCCACCATAGCTCAATGCACGACGAGTATTCTTCCATTGGCTTCTTGTGCACCTTTTGTGCAAGGTGTAACACCAACACCACCTATGGGATGCTGTGATAACCTGAAACAACTCTACAACGTTGTCCCTAACTGCCTTTGCCTCTTGCTGAATGGCACTAACTTAAGCTCTTTCCCTATCAACACTACTAGAGCCCTCCAGCTGCCTGATATTTGCAGCCTTCAAGTTAACATCTCCACTTGTTCAGCTCTCCTTGGGCCACCTAGTCCACCCAGTTCTCATGTTCCTCCTAGAGTACACATCAACTCCACAATTAGCAATGCTACGGTTCTAGCTTCTCCAATGACTCAACCAACACCAAAACCTAGCATTGTGGGTTTAGGATTGGGCAAGATCAGTGCTAGTTTCAAATTAAGGGGAGGAATTCAGCTCACACAGCTAGCAGTGATGTTCTTCTTTATTAGACTTCTACTAGATTGTTTTAGTTAA

Coding sequence (CDS)

ATGGCACACAGACAGCATGTTTCAACTCACTTAAACAATGAACTTTCCCTCTATTTCTACACTGTAAAAGCTAAAGAAAGCTTTATTGACTTTGCAACCATGGCATCTCTTCCACATCTCCCCCTTCCTCAACAAGCTATCTCATACCTAAGTCTGCTGTTTCTCCTGCACGTTCTACCGATCACCATATCACAAGGTCCAACCATATCAACTCCCACCATAGCTCAATGCACGACGAGTATTCTTCCATTGGCTTCTTGTGCACCTTTTGTGCAAGGTGTAACACCAACACCACCTATGGGATGCTGTGATAACCTGAAACAACTCTACAACGTTGTCCCTAACTGCCTTTGCCTCTTGCTGAATGGCACTAACTTAAGCTCTTTCCCTATCAACACTACTAGAGCCCTCCAGCTGCCTGATATTTGCAGCCTTCAAGTTAACATCTCCACTTGTTCAGCTCTCCTTGGGCCACCTAGTCCACCCAGTTCTCATGTTCCTCCTAGAGTACACATCAACTCCACAATTAGCAATGCTACGGTTCTAGCTTCTCCAATGACTCAACCAACACCAAAACCTAGCATTGTGGGTTTAGGATTGGGCAAGATCAGTGCTAGTTTCAAATTAAGGGGAGGAATTCAGCTCACACAGCTAGCAGTGATGTTCTTCTTTATTAGACTTCTACTAGATTGTTTTAGTTAA

Protein sequence

MAHRQHVSTHLNNELSLYFYTVKAKESFIDFATMASLPHLPLPQQAISYLSLLFLLHVLPITISQGPTISTPTIAQCTTSILPLASCAPFVQGVTPTPPMGCCDNLKQLYNVVPNCLCLLLNGTNLSSFPINTTRALQLPDICSLQVNISTCSALLGPPSPPSSHVPPRVHINSTISNATVLASPMTQPTPKPSIVGLGLGKISASFKLRGGIQLTQLAVMFFFIRLLLDCFS*

Homology

[Close]

BLAST of CSPI01G03720 vs. ExPASy Swiss-Prot
Match: Q9C9B1 (Non-specific lipid transfer protein GPI-anchored 10 OS=Arabidopsis thaliana OX=3702 GN=LTPG10 PE=3 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 3.1e-21
Identity = 68/157 (43.31%), Postives = 86/157 (54.78%), Query Frame = 0


Query: 75  AQCTTSILPLASCAPFVQGVTPTPPMGCCDNLKQLYNVVPNCLCLLLNGTNLSS--FPIN 134
           A C + +L LA C PFVQG    P   CCD+L Q+Y+    CLCL LN T+  S  FPIN
Sbjct: 28  ATCASRLLSLAPCGPFVQGFAQLPAQPCCDSLNQIYSQEATCLCLFLNNTSTLSPAFPIN 87

Query: 135 TTRALQLPDICSLQVNISTCSALLGPPSPPSSH---VPPRVHINSTIS-----NATVLAS 194
            T ALQLP +C++  N STCS+     +P  S     PP     S IS     N+ V A+
Sbjct: 88  QTLALQLPPLCNIPANSSTCSSSFPGEAPSDSSSVAPPPSSSTGSQISQGAKNNSRVAAT 147

Query: 195 PMTQPTPKP-SIVGLGLGKISASFKLRGGIQLTQLAV 221
           P+ Q  P+P S +GLG G  S+  K    IQLT  A+
Sbjct: 148 PVAQMAPRPTSFMGLGYGLKSSGSK--SEIQLTIFAL 182

[Close]

BLAST of CSPI01G03720 vs. ExPASy Swiss-Prot
Match: O64864 (Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3702 GN=LTPG13 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 7.1e-10
Identity = 43/134 (32.09%), Postives = 68/134 (50.75%), Query Frame = 0


Query: 76  QCTTSILPLASCAPFVQGVTPTPPMGCCDNLKQLYNVVPNCLCLLLNGTNLS--SFPINT 135
           +CT  ++ +A+C P+VQG   +P   CC  LKQ+ N    CLC+++   N       +N 
Sbjct: 35  ECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQVINSDMKCLCMIIQERNDPDLGLQVNV 94

Query: 136 TRALQLPDICSLQVNISTCSALLG-PPSPPSSHVPPRV--HINSTISNATVLASPMTQPT 195
           + AL LP +C    +I+ C ALL   P+ P + V  ++   +N T+S A+      ++PT
Sbjct: 95  SLALALPSVCHATADITKCPALLHLDPNSPDAQVFYQLAKGLNETVS-ASAPTGSASEPT 154

Query: 196 PKPSIVGLGLGKIS 205
              S  G   G  S
Sbjct: 155 SMSSTPGSSAGNNS 167

[Close]

BLAST of CSPI01G03720 vs. ExPASy Swiss-Prot
Match: O64865 (Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3702 GN=LTPG14 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.5e-09
Identity = 33/94 (35.11%), Postives = 51/94 (54.26%), Query Frame = 0


Query: 76  QCTTSILPLASCAPFVQGVTPTPPMGCCDNLKQLYNVVPNCLCLLLNGTNLS--SFPINT 135
           +CT  ++ +A+C P+VQG   +P   CC  LKQ+ N    CLC+++   N       IN 
Sbjct: 34  ECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQVLNSNKKCLCVIIQDRNDPDLGLQINV 93

Query: 136 TRALQLPDICSLQVNISTCSALLG-PPSPPSSHV 167
           + AL LP +C    +++ C ALL   P+ P + V
Sbjct: 94  SLALALPSVCHAAADVTKCPALLHLDPNSPDAQV 127

[Close]

BLAST of CSPI01G03720 vs. ExPASy Swiss-Prot
Match: Q2PE60 (Non-specific lipid transfer protein GPI-anchored 23 OS=Arabidopsis thaliana OX=3702 GN=LTPG23 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 9.7e-07
Identity = 41/136 (30.15%), Postives = 61/136 (44.85%), Query Frame = 0


Query: 64  SQGPTISTPTIAQCTTSILPLASCAPF--VQGVTPTPPMGCCDNLKQLYNVVPNCLCLLL 123
           S  PT        C++ I  +  C  F  V    P+P   CC  +K + N  P CLC  L
Sbjct: 32  SMAPTPQPSNSTDCSSVIYSMVDCLSFLTVGSTDPSPTKTCCVGVKTVLNYSPKCLCSAL 91

Query: 124 NGTNLSSFPINTTRALQLPDICSLQV----NISTCSALLGPPSPPSSHVPPRVHINSTIS 183
             +    F ++ T+AL +P IC++ +    ++ST +A   P SPP     P    +S  S
Sbjct: 92  ESSREMGFVLDDTKALAMPKICNVPIDPNCDVSTPAAST-PVSPPVE--SPTTSPSSAKS 151

Query: 184 NATVLASPMTQPTPKP 194
            A   +SP    +P P
Sbjct: 152 PAITPSSPAVSHSPPP 164

[Close]

BLAST of CSPI01G03720 vs. ExPASy Swiss-Prot
Match: Q9FY78 (Non-specific lipid transfer protein GPI-anchored 29 OS=Arabidopsis thaliana OX=3702 GN=LTPG29 PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.1e-05
Identity = 34/104 (32.69%), Postives = 53/104 (50.96%), Query Frame = 0


Query: 68  TISTPTI---AQCTTSILPLASCAPFVQ--GVTPTPPMGCCDNLKQLYNVVPNCLCLLLN 127
           ++S+P +   + C T ++ L  C PF+   G   TP   CC +LK + +  P CLC  L 
Sbjct: 16  SVSSPYVHGASDCDTLVITLFPCLPFISIGGTADTPTASCCSSLKNILDTKPICLCEGLK 75

Query: 128 GTNLSSFPINTTRALQLPDICSLQV-NISTCSALLGPPSPPSSH 166
              L    +N T++  LP  C L    +S C + L P SPP+++
Sbjct: 76  KAPL-GIKLNVTKSATLPVACKLNAPPVSACDS-LPPASPPTAN 117

[Close]

BLAST of CSPI01G03720 vs. ExPASy TrEMBL
Match: A0A0A0LSN1 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G022510 PE=4 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 2.1e-105
Identity = 199/200 (99.50%), Postives = 200/200 (100.00%), Query Frame = 0


Query: 34  MASLPHLPLPQQAISYLSLLFLLHVLPITISQGPTISTPTIAQCTTSILPLASCAPFVQG 93
           MASLPHLPLPQQAISYLSLLFLLHVLPITISQGPTISTPTIAQCTTSILPLASCAPFVQG
Sbjct: 1   MASLPHLPLPQQAISYLSLLFLLHVLPITISQGPTISTPTIAQCTTSILPLASCAPFVQG 60

Query: 94  VTPTPPMGCCDNLKQLYNVVPNCLCLLLNGTNLSSFPINTTRALQLPDICSLQVNISTCS 153
           VTPTPPMGCCDNLKQLYNVVPNCLCLLLNGTNLSSFPINTTRALQLPDICSLQVNISTCS
Sbjct: 61  VTPTPPMGCCDNLKQLYNVVPNCLCLLLNGTNLSSFPINTTRALQLPDICSLQVNISTCS 120

Query: 154 ALLGPPSPPSSHVPPRVHINSTISNATVLASPMTQPTPKPSIVGLGLGKISASFKLRGGI 213
           ALLGPPSPPSSHVPPRVHINSTISNATVLASPMTQPTPKPSIVGLGLGKISASFKL+GGI
Sbjct: 121 ALLGPPSPPSSHVPPRVHINSTISNATVLASPMTQPTPKPSIVGLGLGKISASFKLKGGI 180

Query: 214 QLTQLAVMFFFIRLLLDCFS 234
           QLTQLAVMFFFIRLLLDCFS
Sbjct: 181 QLTQLAVMFFFIRLLLDCFS 200

[Close]

BLAST of CSPI01G03720 vs. ExPASy TrEMBL
Match: A0A5A7TH96 (Protein YLS3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00390 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 2.0e-92
Identity = 178/196 (90.82%), Postives = 187/196 (95.41%), Query Frame = 0


Query: 34  MASLPHLPLPQQAISYLSLLFLLHVLPITISQGPTISTPTIAQCTTSILPLASCAPFVQG 93
           MASLP+LPLPQ AISYLSLLFLLHVLPITISQGPTISTPTIAQCTTSILPLASCAPFVQG
Sbjct: 1   MASLPYLPLPQLAISYLSLLFLLHVLPITISQGPTISTPTIAQCTTSILPLASCAPFVQG 60

Query: 94  VTPTPPMGCCDNLKQLYNVVPNCLCLLLNGTNLSSFPINTTRALQLPDICSLQVNISTCS 153
           VTPTPPMGCCDNLKQLYN+VPNCLCLLLN TNLSSFPINTTRALQLPD+CSLQVNISTCS
Sbjct: 61  VTPTPPMGCCDNLKQLYNIVPNCLCLLLNDTNLSSFPINTTRALQLPDLCSLQVNISTCS 120

Query: 154 ALLGPPSPPSSHVPPRVHINSTISNATVLASPMTQPTPKPSIVGLGLGKISASFKLRGGI 213
           ALLG PSPPSSHVPPRVHINSTISNATVLASP+TQPTPKPSIVGLGLGKISASFKLRGGI
Sbjct: 121 ALLGSPSPPSSHVPPRVHINSTISNATVLASPVTQPTPKPSIVGLGLGKISASFKLRGGI 180

Query: 214 QLTQLAVMFFFIRLLL 230
           QLT +  +  F+ L++
Sbjct: 181 QLTAVIAITTFLFLVV 196

[Close]

BLAST of CSPI01G03720 vs. ExPASy TrEMBL
Match: A0A1S3BVJ6 (protein YLS3 OS=Cucumis melo OX=3656 GN=LOC103494119 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 2.0e-92
Identity = 178/196 (90.82%), Postives = 187/196 (95.41%), Query Frame = 0


Query: 34  MASLPHLPLPQQAISYLSLLFLLHVLPITISQGPTISTPTIAQCTTSILPLASCAPFVQG 93
           MASLP+LPLPQ AISYLSLLFLLHVLPITISQGPTISTPTIAQCTTSILPLASCAPFVQG
Sbjct: 1   MASLPYLPLPQLAISYLSLLFLLHVLPITISQGPTISTPTIAQCTTSILPLASCAPFVQG 60

Query: 94  VTPTPPMGCCDNLKQLYNVVPNCLCLLLNGTNLSSFPINTTRALQLPDICSLQVNISTCS 153
           VTPTPPMGCCDNLKQLYN+VPNCLCLLLN TNLSSFPINTTRALQLPD+CSLQVNISTCS
Sbjct: 61  VTPTPPMGCCDNLKQLYNIVPNCLCLLLNDTNLSSFPINTTRALQLPDLCSLQVNISTCS 120

Query: 154 ALLGPPSPPSSHVPPRVHINSTISNATVLASPMTQPTPKPSIVGLGLGKISASFKLRGGI 213
           ALLG PSPPSSHVPPRVHINSTISNATVLASP+TQPTPKPSIVGLGLGKISASFKLRGGI
Sbjct: 121 ALLGSPSPPSSHVPPRVHINSTISNATVLASPVTQPTPKPSIVGLGLGKISASFKLRGGI 180

Query: 214 QLTQLAVMFFFIRLLL 230
           QLT +  +  F+ L++
Sbjct: 181 QLTAVIAITTFLFLVV 196

[Close]

BLAST of CSPI01G03720 vs. ExPASy TrEMBL
Match: A0A6J1E230 (protein YLS3 OS=Cucurbita moschata OX=3662 GN=LOC111430130 PE=4 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 2.7e-68
Identity = 144/197 (73.10%), Postives = 162/197 (82.23%), Query Frame = 0


Query: 34  MASLPHLPLPQQAISYLSLLFLLHV-LPITISQGPTISTPTIAQCTTSILPLASCAPFVQ 93
           MASLP+LP        L+LLFLLHV LPI +SQ PT+S+PTIAQCT S+LPLASCAPF+Q
Sbjct: 1   MASLPYLPC-------LTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFMQ 60

Query: 94  GVTPTPPMGCCDNLKQLYNVVPNCLCLLLNGTNLSSFPINTTRALQLPDICSLQVNISTC 153
           G+   PPM CC NLKQLY  VP+CLCLLLNGT LSSFPINTTRA QLPD+CSLQVNISTC
Sbjct: 61  GMASMPPMTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTC 120

Query: 154 SALLGPPSPPSSHVPPRVHINSTISNATVLASPMTQPTPKPSIVGLGLGKISASFKLRGG 213
           SALL PPSPPSSHV PRV  N+TI+NATVLA+P+ QPTPKPS+VGLGLG ISASFK R G
Sbjct: 121 SALLEPPSPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAG 180

Query: 214 IQLTQLAVMFFFIRLLL 230
           IQLT +  M  F+ LL+
Sbjct: 181 IQLTVVIAMKTFLFLLV 190

[Close]

BLAST of CSPI01G03720 vs. ExPASy TrEMBL
Match: A0A6J1JIC4 (protein YLS3 OS=Cucurbita maxima OX=3661 GN=LOC111484860 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 3.9e-67
Identity = 144/197 (73.10%), Postives = 160/197 (81.22%), Query Frame = 0


Query: 34  MASLPHLPLPQQAISYLSLLFLLHV-LPITISQGPTISTPTIAQCTTSILPLASCAPFVQ 93
           MASLP+LP        L+LL LLHV LPI +SQ PT S+PTIAQCTTS+L LASCAPFVQ
Sbjct: 1   MASLPYLPC-------LTLLLLLHVTLPIAVSQDPTPSSPTIAQCTTSLLQLASCAPFVQ 60

Query: 94  GVTPTPPMGCCDNLKQLYNVVPNCLCLLLNGTNLSSFPINTTRALQLPDICSLQVNISTC 153
           G+  TPPM CC NLKQLY  VP+CLCLLLNGT LSSFPINTTRA QLPD+CSLQVNIS C
Sbjct: 61  GMASTPPMTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISAC 120

Query: 154 SALLGPPSPPSSHVPPRVHINSTISNATVLASPMTQPTPKPSIVGLGLGKISASFKLRGG 213
           SALL PPSPPSSHV PRV+ NSTI+NATVLA+P+ QPTPKPS+VGLGLG ISASFK R G
Sbjct: 121 SALLEPPSPPSSHVSPRVNTNSTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAG 180

Query: 214 IQLTQLAVMFFFIRLLL 230
           IQLT    M  F+ L++
Sbjct: 181 IQLTVAIAMKTFLFLVV 190

[Close]

BLAST of CSPI01G03720 vs. NCBI nr
Match: XP_004138342.2 (protein YLS3 [Cucumis sativus])

HSP 1 Score: 425.6 bits (1093), Expect = 2.7e-115
Identity = 217/229 (94.76%), Postives = 223/229 (97.38%), Query Frame = 0


Query: 1   MAHRQHVSTHLNNELSLYFYTVKAKESFIDFATMASLPHLPLPQQAISYLSLLFLLHVLP 60
           MAHRQHVSTHLNNELSLYFYTVKAKESFIDFATMASLPHLPLPQQAISYLSLLFLLHVLP
Sbjct: 1   MAHRQHVSTHLNNELSLYFYTVKAKESFIDFATMASLPHLPLPQQAISYLSLLFLLHVLP 60

Query: 61  ITISQGPTISTPTIAQCTTSILPLASCAPFVQGVTPTPPMGCCDNLKQLYNVVPNCLCLL 120
           ITISQGPTISTPTIAQCTTSILPLASCAPFVQGVTPTPPMGCCDNLKQLYNVVPNCLCLL
Sbjct: 61  ITISQGPTISTPTIAQCTTSILPLASCAPFVQGVTPTPPMGCCDNLKQLYNVVPNCLCLL 120

Query: 121 LNGTNLSSFPINTTRALQLPDICSLQVNISTCSALLGPPSPPSSHVPPRVHINSTISNAT 180
           LNGTNLSSFPINTTRALQLPDICSLQVNISTCSALLGPPSPPSSHVPPRVHINSTISNAT
Sbjct: 121 LNGTNLSSFPINTTRALQLPDICSLQVNISTCSALLGPPSPPSSHVPPRVHINSTISNAT 180

Query: 181 VLASPMTQPTPKPSIVGLGLGKISASFKLRGGIQLTQLAVMFFFIRLLL 230
           VLASPMTQPTPKPSIVGLGLGKISASFKL+GGIQLT +  +  F+ L++
Sbjct: 181 VLASPMTQPTPKPSIVGLGLGKISASFKLKGGIQLTVVITISTFLFLVV 229

[Close]

BLAST of CSPI01G03720 vs. NCBI nr
Match: KAE8652458.1 (hypothetical protein Csa_013723 [Cucumis sativus])

HSP 1 Score: 362.1 bits (928), Expect = 3.7e-96
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 0


Query: 1   MAHRQHVSTHLNNELSLYFYTVKAKESFIDFATMASLPHLPLPQQAISYLSLLFLLHVLP 60
           MAHRQHVSTHLNNELSLYFYTVKAKESFIDFATMASLPHLPLPQQAISYLSLLFLLHVLP
Sbjct: 1   MAHRQHVSTHLNNELSLYFYTVKAKESFIDFATMASLPHLPLPQQAISYLSLLFLLHVLP 60

Query: 61  ITISQGPTISTPTIAQCTTSILPLASCAPFVQGVTPTPPMGCCDNLKQLYNVVPNCLCLL 120
           ITISQGPTISTPTIAQCTTSILPLASCAPFVQGVTPTPPMGCCDNLKQLYNVVPNCLCLL
Sbjct: 61  ITISQGPTISTPTIAQCTTSILPLASCAPFVQGVTPTPPMGCCDNLKQLYNVVPNCLCLL 120

Query: 121 LNGTNLSSFPINTTRALQLPDICSLQVNISTCSALLGPPSPPSSHVPPRVHINSTISNAT 180
           LNGTNLSSFPINTTRALQLPDICSLQVNISTCSALLGPPSPPSSHVPPRVHINSTISNAT
Sbjct: 121 LNGTNLSSFPINTTRALQLPDICSLQVNISTCSALLGPPSPPSSHVPPRVHINSTISNAT 180

Query: 181 VL 183
           VL
Sbjct: 181 VL 182

[Close]

BLAST of CSPI01G03720 vs. NCBI nr
Match: XP_008453387.1 (PREDICTED: protein YLS3 [Cucumis melo] >KAA0041426.1 protein YLS3 [Cucumis melo var. makuwa] >KAA0058065.1 protein YLS3 [Cucumis melo var. makuwa] >TYK28411.1 protein YLS3 [Cucumis melo var. makuwa])

HSP 1 Score: 348.6 bits (893), Expect = 4.2e-92
Identity = 178/196 (90.82%), Postives = 187/196 (95.41%), Query Frame = 0


Query: 34  MASLPHLPLPQQAISYLSLLFLLHVLPITISQGPTISTPTIAQCTTSILPLASCAPFVQG 93
           MASLP+LPLPQ AISYLSLLFLLHVLPITISQGPTISTPTIAQCTTSILPLASCAPFVQG
Sbjct: 1   MASLPYLPLPQLAISYLSLLFLLHVLPITISQGPTISTPTIAQCTTSILPLASCAPFVQG 60

Query: 94  VTPTPPMGCCDNLKQLYNVVPNCLCLLLNGTNLSSFPINTTRALQLPDICSLQVNISTCS 153
           VTPTPPMGCCDNLKQLYN+VPNCLCLLLN TNLSSFPINTTRALQLPD+CSLQVNISTCS
Sbjct: 61  VTPTPPMGCCDNLKQLYNIVPNCLCLLLNDTNLSSFPINTTRALQLPDLCSLQVNISTCS 120

Query: 154 ALLGPPSPPSSHVPPRVHINSTISNATVLASPMTQPTPKPSIVGLGLGKISASFKLRGGI 213
           ALLG PSPPSSHVPPRVHINSTISNATVLASP+TQPTPKPSIVGLGLGKISASFKLRGGI
Sbjct: 121 ALLGSPSPPSSHVPPRVHINSTISNATVLASPVTQPTPKPSIVGLGLGKISASFKLRGGI 180

Query: 214 QLTQLAVMFFFIRLLL 230
           QLT +  +  F+ L++
Sbjct: 181 QLTAVIAITTFLFLVV 196

[Close]

BLAST of CSPI01G03720 vs. NCBI nr
Match: XP_038890355.1 (non-specific lipid transfer protein GPI-anchored 13 [Benincasa hispida])

HSP 1 Score: 319.7 bits (818), Expect = 2.1e-83
Identity = 164/196 (83.67%), Postives = 178/196 (90.82%), Query Frame = 0


Query: 34  MASLPHLPLPQQAISYLSLLFLLHVLPITISQGPTISTPTIAQCTTSILPLASCAPFVQG 93
           MASLP+LPLPQ AISYLSLLFLLHVLP+ ISQ PT S+PTIAQCTTS+LPLASCA FVQG
Sbjct: 1   MASLPYLPLPQLAISYLSLLFLLHVLPLAISQDPTFSSPTIAQCTTSLLPLASCASFVQG 60

Query: 94  VTPTPPMGCCDNLKQLYNVVPNCLCLLLNGTNLSSFPINTTRALQLPDICSLQVNISTCS 153
           VTPTPPM CCDNLKQLYN VP CLCLLLNGT LSSFPINTTRAL+LPD+CSLQVNIS CS
Sbjct: 61  VTPTPPMVCCDNLKQLYNNVPTCLCLLLNGTTLSSFPINTTRALELPDLCSLQVNISICS 120

Query: 154 ALLGPPSPPSSHVPPRVHINSTISNATVLASPMTQPTPKPSIVGLGLGKISASFKLRGGI 213
           ALLGPPSPPSSHVPPRV+ NSTISNATVLASP+TQPTPKPSIVGLGLG+ISASFKLR GI
Sbjct: 121 ALLGPPSPPSSHVPPRVNTNSTISNATVLASPVTQPTPKPSIVGLGLGRISASFKLRAGI 180

Query: 214 QLTQLAVMFFFIRLLL 230
           Q+T +  M  F+ L++
Sbjct: 181 QVTVVIAMKTFLFLVV 196

[Close]

BLAST of CSPI01G03720 vs. NCBI nr
Match: KAG7023240.1 (Protein YLS3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 295.0 bits (754), Expect = 5.5e-76
Identity = 162/230 (70.43%), Postives = 182/230 (79.13%), Query Frame = 0


Query: 1   MAHRQHVSTHLNNELSLYFYTVKAKESFIDFATMASLPHLPLPQQAISYLSLLFLLHV-L 60
           MAHR H STHLN+ LSLYFY+    ++     TMASLP+LP        L+LLFLLHV L
Sbjct: 1   MAHRLHGSTHLNSVLSLYFYS----QNLHCLCTMASLPYLPC-------LTLLFLLHVLL 60

Query: 61  PITISQGPTISTPTIAQCTTSILPLASCAPFVQGVTPTPPMGCCDNLKQLYNVVPNCLCL 120
           PI +SQ PT+S+PTIAQCT S+LPLASCAPFVQG+   PPM CC NLKQLY  VP+CLCL
Sbjct: 61  PIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQGMASMPPMTCCGNLKQLYTSVPSCLCL 120

Query: 121 LLNGTNLSSFPINTTRALQLPDICSLQVNISTCSALLGPPSPPSSHVPPRVHINSTISNA 180
           LLNGT LSSFPINTTRA QLPD+CSLQVNISTCSALL PPSPPSSHV PRV  N+TI+NA
Sbjct: 121 LLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPPSPPSSHVSPRVDTNTTITNA 180

Query: 181 TVLASPMTQPTPKPSIVGLGLGKISASFKLRGGIQLTQLAVMFFFIRLLL 230
           TVLA+P+ QPTPKPS+VGLGLG ISASFK R GIQLT    M  F+ LL+
Sbjct: 181 TVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVAIAMKTFLFLLV 219

[Close]

BLAST of CSPI01G03720 vs. TAIR 10
Match: AT1G73890.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 103.6 bits (257), Expect = 2.2e-22
Identity = 68/157 (43.31%), Postives = 86/157 (54.78%), Query Frame = 0


Query: 75  AQCTTSILPLASCAPFVQGVTPTPPMGCCDNLKQLYNVVPNCLCLLLNGTNLSS--FPIN 134
           A C + +L LA C PFVQG    P   CCD+L Q+Y+    CLCL LN T+  S  FPIN
Sbjct: 28  ATCASRLLSLAPCGPFVQGFAQLPAQPCCDSLNQIYSQEATCLCLFLNNTSTLSPAFPIN 87

Query: 135 TTRALQLPDICSLQVNISTCSALLGPPSPPSSH---VPPRVHINSTIS-----NATVLAS 194
            T ALQLP +C++  N STCS+     +P  S     PP     S IS     N+ V A+
Sbjct: 88  QTLALQLPPLCNIPANSSTCSSSFPGEAPSDSSSVAPPPSSSTGSQISQGAKNNSRVAAT 147

Query: 195 PMTQPTPKP-SIVGLGLGKISASFKLRGGIQLTQLAV 221
           P+ Q  P+P S +GLG G  S+  K    IQLT  A+
Sbjct: 148 PVAQMAPRPTSFMGLGYGLKSSGSK--SEIQLTIFAL 182

[Close]

BLAST of CSPI01G03720 vs. TAIR 10
Match: AT2G44290.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 65.9 bits (159), Expect = 5.1e-11
Identity = 43/134 (32.09%), Postives = 68/134 (50.75%), Query Frame = 0


Query: 76  QCTTSILPLASCAPFVQGVTPTPPMGCCDNLKQLYNVVPNCLCLLLNGTNLS--SFPINT 135
           +CT  ++ +A+C P+VQG   +P   CC  LKQ+ N    CLC+++   N       +N 
Sbjct: 35  ECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQVINSDMKCLCMIIQERNDPDLGLQVNV 94

Query: 136 TRALQLPDICSLQVNISTCSALLG-PPSPPSSHVPPRV--HINSTISNATVLASPMTQPT 195
           + AL LP +C    +I+ C ALL   P+ P + V  ++   +N T+S A+      ++PT
Sbjct: 95  SLALALPSVCHATADITKCPALLHLDPNSPDAQVFYQLAKGLNETVS-ASAPTGSASEPT 154

Query: 196 PKPSIVGLGLGKIS 205
              S  G   G  S
Sbjct: 155 SMSSTPGSSAGNNS 167

[Close]

BLAST of CSPI01G03720 vs. TAIR 10
Match: AT2G44300.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 63.5 bits (153), Expect = 2.5e-10
Identity = 33/94 (35.11%), Postives = 51/94 (54.26%), Query Frame = 0


Query: 76  QCTTSILPLASCAPFVQGVTPTPPMGCCDNLKQLYNVVPNCLCLLLNGTNLS--SFPINT 135
           +CT  ++ +A+C P+VQG   +P   CC  LKQ+ N    CLC+++   N       IN 
Sbjct: 34  ECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQVLNSNKKCLCVIIQDRNDPDLGLQINV 93

Query: 136 TRALQLPDICSLQVNISTCSALLG-PPSPPSSHV 167
           + AL LP +C    +++ C ALL   P+ P + V
Sbjct: 94  SLALALPSVCHAAADVTKCPALLHLDPNSPDAQV 127

[Close]

BLAST of CSPI01G03720 vs. TAIR 10
Match: AT1G36150.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 55.5 bits (132), Expect = 6.9e-08
Identity = 41/136 (30.15%), Postives = 61/136 (44.85%), Query Frame = 0


Query: 64  SQGPTISTPTIAQCTTSILPLASCAPF--VQGVTPTPPMGCCDNLKQLYNVVPNCLCLLL 123
           S  PT        C++ I  +  C  F  V    P+P   CC  +K + N  P CLC  L
Sbjct: 32  SMAPTPQPSNSTDCSSVIYSMVDCLSFLTVGSTDPSPTKTCCVGVKTVLNYSPKCLCSAL 91

Query: 124 NGTNLSSFPINTTRALQLPDICSLQV----NISTCSALLGPPSPPSSHVPPRVHINSTIS 183
             +    F ++ T+AL +P IC++ +    ++ST +A   P SPP     P    +S  S
Sbjct: 92  ESSREMGFVLDDTKALAMPKICNVPIDPNCDVSTPAAST-PVSPPVE--SPTTSPSSAKS 151

Query: 184 NATVLASPMTQPTPKP 194
            A   +SP    +P P
Sbjct: 152 PAITPSSPAVSHSPPP 164

[Close]

BLAST of CSPI01G03720 vs. TAIR 10
Match: AT3G22600.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 52.0 bits (123), Expect = 7.6e-07
Identity = 30/97 (30.93%), Postives = 52/97 (53.61%), Query Frame = 0


Query: 61  ITISQGPTISTPTIAQ--CTTSILPLASCAPFVQGVTPTPPMGCCDNLKQLYNVVPNCLC 120
           +T+      ST   AQ  CT +++ ++ C  ++ G + +P   CC+ L ++    P+CLC
Sbjct: 10  LTVFMAVMSSTMVSAQSSCTNALISMSPCLNYITGNSTSPNQQCCNQLSRVVQSSPDCLC 69

Query: 121 LLLNGTNLS-SFPINTTRALQLPDICSLQV-NISTCS 154
            +LNG        +N T+AL LP  C++Q   +S C+
Sbjct: 70  QVLNGGGSQLGINVNQTQALGLPRACNVQTPPVSRCN 106

The following BLAST results are available for this feature:

BLAST of CSPI01G03720 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C9B1	3.1e-21	43.31	Non-specific lipid transfer protein GPI-anchored 10 OS=Arabidopsis thaliana OX=3...	[more]
O64864	7.1e-10	32.09	Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3...	[more]
O64865	3.5e-09	35.11	Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3...	[more]
Q2PE60	9.7e-07	30.15	Non-specific lipid transfer protein GPI-anchored 23 OS=Arabidopsis thaliana OX=3...	[more]
Q9FY78	1.1e-05	32.69	Non-specific lipid transfer protein GPI-anchored 29 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of CSPI01G03720 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LSN1	2.1e-105	99.50	AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G022510 PE=4 SV...	[more]
A0A5A7TH96	2.0e-92	90.82	Protein YLS3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00390 P...	[more]
A0A1S3BVJ6	2.0e-92	90.82	protein YLS3 OS=Cucumis melo OX=3656 GN=LOC103494119 PE=4 SV=1	[more]
A0A6J1E230	2.7e-68	73.10	protein YLS3 OS=Cucurbita moschata OX=3662 GN=LOC111430130 PE=4 SV=1	[more]
A0A6J1JIC4	3.9e-67	73.10	protein YLS3 OS=Cucurbita maxima OX=3661 GN=LOC111484860 PE=4 SV=1	[more]

BLAST of CSPI01G03720 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138342.2	2.7e-115	94.76	protein YLS3 [Cucumis sativus]	[more]
KAE8652458.1	3.7e-96	100.00	hypothetical protein Csa_013723 [Cucumis sativus]	[more]
XP_008453387.1	4.2e-92	90.82	PREDICTED: protein YLS3 [Cucumis melo] >KAA0041426.1 protein YLS3 [Cucumis melo ...	[more]
XP_038890355.1	2.1e-83	83.67	non-specific lipid transfer protein GPI-anchored 13 [Benincasa hispida]	[more]
KAG7023240.1	5.5e-76	70.43	Protein YLS3, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of CSPI01G03720 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73890.1	2.2e-22	43.31	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT2G44290.1	5.1e-11	32.09	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT2G44300.1	2.5e-10	35.11	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G36150.1	6.9e-08	30.15	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT3G22600.1	7.6e-07	30.93	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	SMART	SM00499	aai_6	coord: 77..152 e-value: 3.2E-4 score: 30.0
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	PFAM	PF14368	LTP_2	coord: 67..152 e-value: 1.8E-14 score: 53.6
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	GENE3D	1.10.110.10		coord: 73..152 e-value: 3.8E-15 score: 57.5
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	SUPERFAMILY	47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 76..152
IPR043325	Alpha-Amylase Inhibitors (AAI), Lipid Transfer (LT) and Seed Storage (SS) Protein	PANTHER	PTHR33044	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED	coord: 54..178
None	No IPR available	PANTHER	PTHR33044:SF11	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN	coord: 54..178
None	No IPR available	CDD	cd00010	AAI_LTSS	coord: 84..145 e-value: 2.10124E-14 score: 63.985

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G03720.1	CSPI01G03720.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane