Bhi12G001461 (gene) Wax gourd (B227) v1

Overview
NameBhi12G001461
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionserine/threonine-protein kinase BLUS1-like
Locationchr12: 53276253 .. 53277845 (-)
RNA-Seq ExpressionBhi12G001461
SyntenyBhi12G001461
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCAGCATCAAGTTCAAGAATCGGCCACCACAACCGCCAATAGTACACTGAGTTTCAAGTTTCCGACTACCTCTAATGGCTACAAAATCATCACTCAGATCGGTGTCAGCCCCCACAGCATTGTATACATGGCCAATTGCAACTCTGAAACCGTCGCCGTGAAAATCATCGATATCAATATCAACCTCAACCTTGATTCCCTAAATACTTCACTATCACTGATCGACCACCCCAATATTCTCCGCCCACATTGTTGTTTCATCGGCGAAGACGGCCGAATTTGGATCGTAATGCCGTTTATTCCAACCGGGCCTTTACAATCCATTCTGTCCCAAATTTTCCCAAGAGGTATGCCAGATCATTTCATCTCCATTCTTTTAAATCAACTCCTTCGTGCTCTGGTTTATCTTCACGAATTGGGTTTTGTTCATAAAGCCATTAAACCAAGTAATATCTTCTTTGATTCACAAGGCTCTGTTAAGCTCTCTGTTTTTGACATTATGATATCCAAGAACAGTACTAATGAAATTGATCATGATTCTTCAATTCCTTATTGGGTTCCACCGGATGAAGAATATAGTTTCAAATCAGACGCTTGGGCAGTTGGGGTTTTAGCTATGGAAATTACTTGGGGGGGTCCTCCAATTATAGACCCAAGAATTCTTGAATCACAATTCTTCAATATCTCAAGAAGGTTTCCTTATGAACCGAAAGCAGAGAGGAGTCGTGACAGAAGATTATTTGGAGACTCTGTTAAAGAATTTGTGAAATTTTGCCTCGTGAAAGATCAGTTTGGAAGGCCTACGGCTTTGAGTTTGTTGGAACATCCATTTGTGAAGAAAGGGGAAAACTCGAATTTTTTGTTCAAGTGTATTCCAAAAGGAGTGATTGAAATTGATAAAATATTTGAACAAGAAAAGGAAAGAATCATTGGGTTGATGAAAAATGATGAGAAATTGAAGAACAAGAGTGAAGGGGACAAGATTATGAAGATTAGTGGATGGGATTATAATAATTTTTTGTTTGAACTGGAACCTGATGAGGAATCAGTAGGGAAAAGAGTTCGATTTGGTGGGGAAGTCGTAAGACAATTTAAGAAAGATGAAGATGAAGATAAAGATGAAGATGAAGAAGAAGATGAAGAAGAAGAAGAAGAAGAGAGCAAGAAAGTTGATGATATGTCACCTTTACTTCAAAAAATAAAGAGTAATTTGAGTGGTGTTGGTGAATCAAGCAAGAAGGCTGGTGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAAAATGTTGATGAAGGAAGTTTAGTGAAAAAGCTGTGTTCATTGTTAGATAATATTGATGAACAAAGAGAGATGGTTTTGGTGTTGATAAATCATATTGGTTATTTGACTGAAAAACAGTTGGAAATGAGTAATGAAATCAAAAAGTTGGAGGCAGATTTGGCTTCTGAGAAAGAAAGATACTTTCAATTAGAGTTAGAATGTGAATTTCTAAAGCTCAAAGTTCCTGATTCTGAGGATGAAAATGAATATTCAGATGAGGAACAACAATATTATGGGGATGAGGATGAACAACCAGATTCTCAAGAGTGA

mRNA sequence

ATGAAGCAGCATCAAGTTCAAGAATCGGCCACCACAACCGCCAATAGTACACTGAGTTTCAAGTTTCCGACTACCTCTAATGGCTACAAAATCATCACTCAGATCGGTGTCAGCCCCCACAGCATTGTATACATGGCCAATTGCAACTCTGAAACCGTCGCCGTGAAAATCATCGATATCAATATCAACCTCAACCTTGATTCCCTAAATACTTCACTATCACTGATCGACCACCCCAATATTCTCCGCCCACATTGTTGTTTCATCGGCGAAGACGGCCGAATTTGGATCGTAATGCCGTTTATTCCAACCGGGCCTTTACAATCCATTCTGTCCCAAATTTTCCCAAGAGGTATGCCAGATCATTTCATCTCCATTCTTTTAAATCAACTCCTTCGTGCTCTGGTTTATCTTCACGAATTGGGTTTTGTTCATAAAGCCATTAAACCAAGTAATATCTTCTTTGATTCACAAGGCTCTGTTAAGCTCTCTGTTTTTGACATTATGATATCCAAGAACAGTACTAATGAAATTGATCATGATTCTTCAATTCCTTATTGGGTTCCACCGGATGAAGAATATAGTTTCAAATCAGACGCTTGGGCAGTTGGGGTTTTAGCTATGGAAATTACTTGGGGGGGTCCTCCAATTATAGACCCAAGAATTCTTGAATCACAATTCTTCAATATCTCAAGAAGGTTTCCTTATGAACCGAAAGCAGAGAGGAGTCGTGACAGAAGATTATTTGGAGACTCTGTTAAAGAATTTGTGAAATTTTGCCTCGTGAAAGATCAGTTTGGAAGGCCTACGGCTTTGAGTTTGTTGGAACATCCATTTGTGAAGAAAGGGGAAAACTCGAATTTTTTGTTCAAGTGTATTCCAAAAGGAGTGATTGAAATTGATAAAATATTTGAACAAGAAAAGGAAAGAATCATTGGGTTGATGAAAAATGATGAGAAATTGAAGAACAAGAGTGAAGGGGACAAGATTATGAAGATTAGTGGATGGGATTATAATAATTTTTTGTTTGAACTGGAACCTGATGAGGAATCAGTAGGGAAAAGAGTTCGATTTGGTGGGGAAGTCGTAAGACAATTTAAGAAAGATGAAGATGAAGATAAAGATGAAGATGAAGAAGAAGATGAAGAAGAAGAAGAAGAAGAGAGCAAGAAAGTTGATGATATGTCACCTTTACTTCAAAAAATAAAGAGTAATTTGAGTGGTGTTGGTGAATCAAGCAAGAAGGCTGGTGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAAAATGTTGATGAAGGAAGTTTAGTGAAAAAGCTGTGTTCATTGTTAGATAATATTGATGAACAAAGAGAGATGGTTTTGGTGTTGATAAATCATATTGGTTATTTGACTGAAAAACAGTTGGAAATGAGTAATGAAATCAAAAAGTTGGAGGCAGATTTGGCTTCTGAGAAAGAAAGATACTTTCAATTAGAGTTAGAATGTGAATTTCTAAAGCTCAAAGTTCCTGATTCTGAGGATGAAAATGAATATTCAGATGAGGAACAACAATATTATGGGGATGAGGATGAACAACCAGATTCTCAAGAGTGA

Coding sequence (CDS)

ATGAAGCAGCATCAAGTTCAAGAATCGGCCACCACAACCGCCAATAGTACACTGAGTTTCAAGTTTCCGACTACCTCTAATGGCTACAAAATCATCACTCAGATCGGTGTCAGCCCCCACAGCATTGTATACATGGCCAATTGCAACTCTGAAACCGTCGCCGTGAAAATCATCGATATCAATATCAACCTCAACCTTGATTCCCTAAATACTTCACTATCACTGATCGACCACCCCAATATTCTCCGCCCACATTGTTGTTTCATCGGCGAAGACGGCCGAATTTGGATCGTAATGCCGTTTATTCCAACCGGGCCTTTACAATCCATTCTGTCCCAAATTTTCCCAAGAGGTATGCCAGATCATTTCATCTCCATTCTTTTAAATCAACTCCTTCGTGCTCTGGTTTATCTTCACGAATTGGGTTTTGTTCATAAAGCCATTAAACCAAGTAATATCTTCTTTGATTCACAAGGCTCTGTTAAGCTCTCTGTTTTTGACATTATGATATCCAAGAACAGTACTAATGAAATTGATCATGATTCTTCAATTCCTTATTGGGTTCCACCGGATGAAGAATATAGTTTCAAATCAGACGCTTGGGCAGTTGGGGTTTTAGCTATGGAAATTACTTGGGGGGGTCCTCCAATTATAGACCCAAGAATTCTTGAATCACAATTCTTCAATATCTCAAGAAGGTTTCCTTATGAACCGAAAGCAGAGAGGAGTCGTGACAGAAGATTATTTGGAGACTCTGTTAAAGAATTTGTGAAATTTTGCCTCGTGAAAGATCAGTTTGGAAGGCCTACGGCTTTGAGTTTGTTGGAACATCCATTTGTGAAGAAAGGGGAAAACTCGAATTTTTTGTTCAAGTGTATTCCAAAAGGAGTGATTGAAATTGATAAAATATTTGAACAAGAAAAGGAAAGAATCATTGGGTTGATGAAAAATGATGAGAAATTGAAGAACAAGAGTGAAGGGGACAAGATTATGAAGATTAGTGGATGGGATTATAATAATTTTTTGTTTGAACTGGAACCTGATGAGGAATCAGTAGGGAAAAGAGTTCGATTTGGTGGGGAAGTCGTAAGACAATTTAAGAAAGATGAAGATGAAGATAAAGATGAAGATGAAGAAGAAGATGAAGAAGAAGAAGAAGAAGAGAGCAAGAAAGTTGATGATATGTCACCTTTACTTCAAAAAATAAAGAGTAATTTGAGTGGTGTTGGTGAATCAAGCAAGAAGGCTGGTGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAAAATGTTGATGAAGGAAGTTTAGTGAAAAAGCTGTGTTCATTGTTAGATAATATTGATGAACAAAGAGAGATGGTTTTGGTGTTGATAAATCATATTGGTTATTTGACTGAAAAACAGTTGGAAATGAGTAATGAAATCAAAAAGTTGGAGGCAGATTTGGCTTCTGAGAAAGAAAGATACTTTCAATTAGAGTTAGAATGTGAATTTCTAAAGCTCAAAGTTCCTGATTCTGAGGATGAAAATGAATATTCAGATGAGGAACAACAATATTATGGGGATGAGGATGAACAACCAGATTCTCAAGAGTGA

Protein sequence

MKQHQVQESATTTANSTLSFKFPTTSNGYKIITQIGVSPHSIVYMANCNSETVAVKIIDININLNLDSLNTSLSLIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQSILSQIFPRGMPDHFISILLNQLLRALVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMISKNSTNEIDHDSSIPYWVPPDEEYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNISRRFPYEPKAERSRDRRLFGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGENSNFLFKCIPKGVIEIDKIFEQEKERIIGLMKNDEKLKNKSEGDKIMKISGWDYNNFLFELEPDEESVGKRVRFGGEVVRQFKKDEDEDKDEDEEEDEEEEEEESKKVDDMSPLLQKIKSNLSGVGESSKKAGEKKKKKKKKKKNVDEGSLVKKLCSLLDNIDEQREMVLVLINHIGYLTEKQLEMSNEIKKLEADLASEKERYFQLELECEFLKLKVPDSEDENEYSDEEQQYYGDEDEQPDSQE
Homology
BLAST of Bhi12G001461 vs. TAIR 10
Match: AT4G14480.1 (Protein kinase superfamily protein )

HSP 1 Score: 184.9 bits (468), Expect = 1.7e-46
Identity = 146/499 (29.26%), Postives = 247/499 (49.50%), Query Frame = 0

Query: 21  KFPTTSNGYKIITQIGVSPHSIVYMANC---NSETVAVKIIDIN-INLNLDSL---NTSL 80
           +FP  +  Y+II +IGV   + VY A C   NS  VA+K ID++    + DSL     ++
Sbjct: 7   EFPLDAEAYEIICKIGVGVSASVYKAICIPMNSMVVAIKAIDLDQSRADFDSLRRETKTM 66

Query: 81  SLIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQSILSQIFPRGMPDHFISILLNQLLR 140
           SL+ HPNIL  +C F   D  +W+VMPF+  G L SI+S  FP G+P++ IS+ L + L 
Sbjct: 67  SLLSHPNILNAYCSFT-VDRCLWVVMPFMSCGSLHSIVSSSFPSGLPENCISVFLKETLN 126

Query: 141 ALVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMIS-----------KNSTNEIDHDS 200
           A+ YLH+ G +H+ IK  NI  DS GSVKL+ F +  S            +S+  +   +
Sbjct: 127 AISYLHDQGHLHRDIKAGNILVDSDGSVKLADFGVSASIYEPVTSSSGTTSSSLRLTDIA 186

Query: 201 SIPYWVPPD-----EEYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNISRRFPY- 260
             PYW+ P+       Y FK+D W+ G+ A+E+  G PP+     L+S    I++RF + 
Sbjct: 187 GTPYWMAPEVVHSHTGYGFKADIWSFGITALELAHGRPPLSHLPPLKSLLMKITKRFHFS 246

Query: 261 ----EPKAERSRDRRLFGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGENSNFLFKC 320
                      +  + F  + +E V  CL +D   RP+A  LL+HPF K  +  +F+ K 
Sbjct: 247 DYEINTSGSSKKGNKKFSKAFREMVGLCLEQDPTKRPSAEKLLKHPFFKNCKGLDFVVKN 306

Query: 321 IPKGVIEIDKIFEQEKERIIGLMKNDEKLKNKSEGDKIMK---ISGWDYNNFLFELEPDE 380
           +   +   +++F + +  I  +  +DE  + + E ++I+K   ISGW++     +L P  
Sbjct: 307 VLHSLSNAEQMFMESQILIKSVGDDDE--EEEEEDEEIVKNRRISGWNFREDDLQLSPVF 366

Query: 381 ESVGKRVRFGGEVVRQFKKDEDEDKDEDEEEDEEEEEEESKKVDDMSPLLQKIKSNLSGV 440
            +                     + D  E    EE++ + KK DD           ++G 
Sbjct: 367 PAT--------------------ESDSSESSPREEDQSKDKKEDD--------NVTITGY 426

Query: 441 GESSKKAGEKKKKKKKKKKNVDEGSLVKKLCSLLDNIDEQREMVLVLINHIGYLTE---K 486
                 + E+ K ++ +    D+  +++KL  L  +++ QR  V ++I  +    E   +
Sbjct: 427 ELGLGLSNEEAKNQEGEVVGFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKEEKSR 474

BLAST of Bhi12G001461 vs. TAIR 10
Match: AT1G23700.1 (Protein kinase superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 4.1e-40
Identity = 109/330 (33.03%), Postives = 174/330 (52.73%), Query Frame = 0

Query: 21  KFPTTSNGYKIITQIGVSPHSIVYMANC--NSETVAVKIIDININLN-LDSLNTS---LS 80
           +FP  +  Y+I+ +IG      VY A C    E VA+KI ++    N L+++      LS
Sbjct: 8   RFPLVAKDYEILEEIGDG----VYRARCILLDEIVAIKIWNLEKCTNDLETIRKEVHRLS 67

Query: 81  LIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQSILSQIFPRGMPDHFISILLNQLLRA 140
           LIDHPN+LR HC FI +   +WIVMPF+  G   +I+  ++P G+ +  I+ILL ++L+A
Sbjct: 68  LIDHPNLLRVHCSFI-DSSSLWIVMPFMSCGSSLNIMKSVYPNGLEEPVIAILLREILKA 127

Query: 141 LVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMISKNSTNEIDHDSS------IPYWV 200
           LVYLH LG +H+ +K  N+  DS+G+VKL  F++  S   + E    SS       P  +
Sbjct: 128 LVYLHGLGHIHRNVKAGNVLVDSEGTVKLGDFEVSASMFDSVERMRTSSENTFVGNPRRM 187

Query: 201 PPDEE------YSFKSDAWAVGVLAMEITWGGPP--IIDPRILESQFFNISRRFPYEPKA 260
            P+++      Y FK D W+ G+ A+E+  G  P  ++   +  S F N      YE   
Sbjct: 188 APEKDMQQVDGYDFKVDIWSFGMTALELAHGHSPTTVLPLNLQNSPFPN------YEEDT 247

Query: 261 ERSRDRRLFGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGENSNFLFKCIPKGVIEI 320
           +       F  S +E V  CL++D   RPTA  LLE+PF+++  ++ +L      G+  +
Sbjct: 248 K-------FSKSFRELVAACLIEDPEKRPTASQLLEYPFLQQTLSTEYLASTFLDGLSPL 307

Query: 321 DKIFEQEKERIIGLMKNDEKLKNKSEGDKI 331
            + + + KE    L+K  +  K K   + +
Sbjct: 308 GERYRKLKEEKAKLVKGVDGNKEKVSQENV 319

BLAST of Bhi12G001461 vs. TAIR 10
Match: AT1G79640.1 (Protein kinase superfamily protein )

HSP 1 Score: 154.1 bits (388), Expect = 3.2e-37
Identity = 97/338 (28.70%), Postives = 166/338 (49.11%), Query Frame = 0

Query: 21  KFPTTSNGYKIITQIGVSPHSIVYMANC--NSETVAVKIIDI---NINLNLDSLNT-SLS 80
           K+P     Y +   IG    ++V+ A C    E VA+KI+D    N +LN  S    ++ 
Sbjct: 8   KYPIGPEHYTLYEFIGQGVSALVHRALCIPFDEVVAIKILDFERDNCDLNNISREAQTMM 67

Query: 81  LIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQSILSQIFPRGMPDHFISILLNQLLRA 140
           L+DHPN+L+ HC F+  D  +W++MP++  G    IL   +P G  +  I+ +L + L+ 
Sbjct: 68  LVDHPNVLKSHCSFV-SDHNLWVIMPYMSGGSCLHILKAAYPDGFEEAIIATILREALKG 127

Query: 141 LVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMISKNSTNEIDHDSS----IPYWVPP 200
           L YLH+ G +H+ +K  NI   ++G+VKL  F +      + +     +     P W+ P
Sbjct: 128 LDYLHQHGHIHRDVKAGNILLGARGAVKLGDFGVSACLFDSGDRQRTRNTFVGTPCWMAP 187

Query: 201 D-----EEYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNISRRFPYEPKAERSRD 260
           +       Y FK+D W+ G+  +E+  G  P      ++     +       P  +  RD
Sbjct: 188 EVMEQLHGYDFKADIWSFGITGLELAHGHAPFSKYPPMKVLLMTLQNA---PPGLDYERD 247

Query: 261 RRLFGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGENSNF----LFKCIPKGVIEID 320
           ++ F  S K+ +  CLVKD   RP+A  LL+H F K+  +S++    L   +P  V  + 
Sbjct: 248 KK-FSRSFKQMIASCLVKDPSKRPSAKKLLKHSFFKQARSSDYIARKLLDGLPDLVNRVQ 307

Query: 321 KIFEQEKERIIGLMKNDEKLKNKSEGDKIMKISGWDYN 340
            I  +E++ +      D + +  S+ +    ISGW++N
Sbjct: 308 AIKRKEEDMLAQEKMADGEKEELSQNEYKRGISGWNFN 340

BLAST of Bhi12G001461 vs. TAIR 10
Match: AT1G79640.2 (Protein kinase superfamily protein )

HSP 1 Score: 154.1 bits (388), Expect = 3.2e-37
Identity = 97/338 (28.70%), Postives = 166/338 (49.11%), Query Frame = 0

Query: 21  KFPTTSNGYKIITQIGVSPHSIVYMANC--NSETVAVKIIDI---NINLNLDSLNT-SLS 80
           K+P     Y +   IG    ++V+ A C    E VA+KI+D    N +LN  S    ++ 
Sbjct: 5   KYPIGPEHYTLYEFIGQGVSALVHRALCIPFDEVVAIKILDFERDNCDLNNISREAQTMM 64

Query: 81  LIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQSILSQIFPRGMPDHFISILLNQLLRA 140
           L+DHPN+L+ HC F+  D  +W++MP++  G    IL   +P G  +  I+ +L + L+ 
Sbjct: 65  LVDHPNVLKSHCSFV-SDHNLWVIMPYMSGGSCLHILKAAYPDGFEEAIIATILREALKG 124

Query: 141 LVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMISKNSTNEIDHDSS----IPYWVPP 200
           L YLH+ G +H+ +K  NI   ++G+VKL  F +      + +     +     P W+ P
Sbjct: 125 LDYLHQHGHIHRDVKAGNILLGARGAVKLGDFGVSACLFDSGDRQRTRNTFVGTPCWMAP 184

Query: 201 D-----EEYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNISRRFPYEPKAERSRD 260
           +       Y FK+D W+ G+  +E+  G  P      ++     +       P  +  RD
Sbjct: 185 EVMEQLHGYDFKADIWSFGITGLELAHGHAPFSKYPPMKVLLMTLQNA---PPGLDYERD 244

Query: 261 RRLFGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGENSNF----LFKCIPKGVIEID 320
           ++ F  S K+ +  CLVKD   RP+A  LL+H F K+  +S++    L   +P  V  + 
Sbjct: 245 KK-FSRSFKQMIASCLVKDPSKRPSAKKLLKHSFFKQARSSDYIARKLLDGLPDLVNRVQ 304

Query: 321 KIFEQEKERIIGLMKNDEKLKNKSEGDKIMKISGWDYN 340
            I  +E++ +      D + +  S+ +    ISGW++N
Sbjct: 305 AIKRKEEDMLAQEKMADGEKEELSQNEYKRGISGWNFN 337

BLAST of Bhi12G001461 vs. TAIR 10
Match: AT1G70430.1 (Protein kinase superfamily protein )

HSP 1 Score: 153.3 bits (386), Expect = 5.5e-37
Identity = 106/336 (31.55%), Postives = 173/336 (51.49%), Query Frame = 0

Query: 21  KFPTTSNGYKIITQIGVSPHSIVYMANC--NSETVAVKIIDININLN-LDSLNTS---LS 80
           +FP  +  Y++  ++G    + VY A C   +E VAVKI+D+    N L+++      +S
Sbjct: 8   RFPLYAKDYELFEEVGEGVSATVYRARCIALNEIVAVKILDLEKCRNDLETIRKEVHIMS 67

Query: 81  LIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQSILSQIFPRGMPDHFISILLNQLLRA 140
           LIDHPN+L+ HC FI +   +WIVMP++  G    ++  ++P G+    I+ LL ++L+A
Sbjct: 68  LIDHPNLLKAHCSFI-DSSSLWIVMPYMSGGSCFHLMKSVYPEGLEQPIIATLLREVLKA 127

Query: 141 LVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMISKNSTNEIDHDSS----IPYWVPP 200
           LVYLH  G +H+ +K  NI   S+G VKL  F +      + E     +     P W+ P
Sbjct: 128 LVYLHRQGHIHRDVKAGNILIHSKGVVKLGDFGVSACMFDSGERMQTRNTFVGTPCWMAP 187

Query: 201 D-----EEYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNISRRFPYEPKAERSRD 260
           +     + Y FK  A      +       PP+   ++L     N   R  Y+      RD
Sbjct: 188 EVMQQLDGYDFKYLAHGHAPFSKY-----PPM---KVLLMTLQNAPPRLDYD------RD 247

Query: 261 RRLFGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGENSNFLFKCIPKGVIEIDKIFE 320
           ++ F  S +E +  CLVKD   RPTA  LL+HPF K   ++++L + I  G+  + + F+
Sbjct: 248 KK-FSKSFRELIAACLVKDPKKRPTAAKLLKHPFFKHARSTDYLSRKILHGLSPLGERFK 307

Query: 321 QEKERIIGLMK--NDEKLKNKSEGDKIMKISGWDYN 340
           + KE    L K  N +K +  S+ + +  IS W+++
Sbjct: 308 KLKEAEAELFKGINGDK-EQLSQHEYMRGISAWNFD 326

BLAST of Bhi12G001461 vs. ExPASy Swiss-Prot
Match: O23304 (Serine/threonine-protein kinase BLUS1 OS=Arabidopsis thaliana OX=3702 GN=BLUS1 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 2.4e-45
Identity = 146/499 (29.26%), Postives = 247/499 (49.50%), Query Frame = 0

Query: 21  KFPTTSNGYKIITQIGVSPHSIVYMANC---NSETVAVKIIDIN-INLNLDSL---NTSL 80
           +FP  +  Y+II +IGV   + VY A C   NS  VA+K ID++    + DSL     ++
Sbjct: 7   EFPLDAEAYEIICKIGVGVSASVYKAICIPMNSMVVAIKAIDLDQSRADFDSLRRETKTM 66

Query: 81  SLIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQSILSQIFPRGMPDHFISILLNQLLR 140
           SL+ HPNIL  +C F   D  +W+VMPF+  G L SI+S  FP G+P++ IS+ L + L 
Sbjct: 67  SLLSHPNILNAYCSFT-VDRCLWVVMPFMSCGSLHSIVSSSFPSGLPENCISVFLKETLN 126

Query: 141 ALVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMIS-----------KNSTNEIDHDS 200
           A+ YLH+ G +H+ IK  NI  DS GSVKL+ F +  S            +S+  +   +
Sbjct: 127 AISYLHDQGHLHRDIKAGNILVDSDGSVKLADFGVSASIYEPVTSSSGTTSSSLRLTDIA 186

Query: 201 SIPYWVPPD-----EEYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNISRRFPY- 260
             PYW+ P+       Y FK+D W+ G+ A+E+  G PP+     L+S    I++RF + 
Sbjct: 187 GTPYWMAPEVVHSHTGYGFKADIWSFGITALELAHGRPPLSHLPPLKSLLMKITKRFHFS 246

Query: 261 ----EPKAERSRDRRLFGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGENSNFLFKC 320
                      +  + F  + +E V  CL +D   RP+A  LL+HPF K  +  +F+ K 
Sbjct: 247 DYEINTSGSSKKGNKKFSKAFREMVGLCLEQDPTKRPSAEKLLKHPFFKNCKGLDFVVKN 306

Query: 321 IPKGVIEIDKIFEQEKERIIGLMKNDEKLKNKSEGDKIMK---ISGWDYNNFLFELEPDE 380
           +   +   +++F + +  I  +  +DE  + + E ++I+K   ISGW++     +L P  
Sbjct: 307 VLHSLSNAEQMFMESQILIKSVGDDDE--EEEEEDEEIVKNRRISGWNFREDDLQLSPVF 366

Query: 381 ESVGKRVRFGGEVVRQFKKDEDEDKDEDEEEDEEEEEEESKKVDDMSPLLQKIKSNLSGV 440
            +                     + D  E    EE++ + KK DD           ++G 
Sbjct: 367 PAT--------------------ESDSSESSPREEDQSKDKKEDD--------NVTITGY 426

Query: 441 GESSKKAGEKKKKKKKKKKNVDEGSLVKKLCSLLDNIDEQREMVLVLINHIGYLTE---K 486
                 + E+ K ++ +    D+  +++KL  L  +++ QR  V ++I  +    E   +
Sbjct: 427 ELGLGLSNEEAKNQEGEVVGFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKEEKSR 474

BLAST of Bhi12G001461 vs. ExPASy Swiss-Prot
Match: Q551H4 (Serine/threonine-protein kinase fray2 OS=Dictyostelium discoideum OX=44689 GN=fray2 PE=3 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 8.0e-33
Identity = 90/326 (27.61%), Postives = 162/326 (49.69%), Query Frame = 0

Query: 9   SATTTANSTLSFK----FPTTSNGYKIITQIGVSPHSIVYMANC--NSETVAVKIIDIN- 68
           S TTTA S ++ K    +P +++ Y++   IG     +V  A C    E VA+KIID+  
Sbjct: 47  STTTTAASPITPKPRKNYPNSADQYELKETIGKGGSGLVQRAICLPFQENVAIKIIDLEH 106

Query: 69  -INLNLDSLN---TSLSLIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQSILSQIFPR 128
             N++L+ +     ++SL  HPN++  H  F+  +  +W++M F+  G    I+   FP+
Sbjct: 107 CKNVSLEEIRKEIQAMSLCHHPNVVAYHTSFVYNES-LWVIMDFLSAGSCSDIMRFSFPQ 166

Query: 129 GMPDHFISILLNQLLRALVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMISKNSTNE 188
           G  +H I+ +L + L+A+ Y H+ G +H+ IK  NI  DS G+++LS F +  +   T E
Sbjct: 167 GFEEHVIATILKEALKAICYFHKTGRIHRDIKSGNILIDSNGNIQLSDFGVSATLIDTGE 226

Query: 189 IDHDSSI--PYWVPPD----EEYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNIS 248
              ++ +  P W+ P+      Y +  D W+ G+ A+E+  G  P  +   ++     + 
Sbjct: 227 TSRNTFVGTPCWMAPEIMEQVNYDYAVDIWSFGITALELARGKAPFAEYPPMKVLLLTLQ 286

Query: 249 RRFPYEPKAERSRDRRLFGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGENSNFLFK 308
                 P +        +  S K+ V+ CL KD   RP    LLEH F K+ +  ++L +
Sbjct: 287 N----PPPSLEGDGESKWSHSFKDLVEKCLQKDPSKRPLPSKLLEHRFFKQAKKPDYLVQ 346

Query: 309 CIPKGVIEIDKIFEQEKERIIGLMKN 318
            I   +  + + ++   E    +++N
Sbjct: 347 HILAKLPPLGQRYQMLSEDSFAMLRN 367

BLAST of Bhi12G001461 vs. ExPASy Swiss-Prot
Match: Q55GV3 (Serine/threonine-protein kinase pakC OS=Dictyostelium discoideum OX=44689 GN=pakC PE=1 SV=2)

HSP 1 Score: 117.1 bits (292), Expect = 6.1e-25
Identity = 82/262 (31.30%), Postives = 131/262 (50.00%), Query Frame = 0

Query: 29  YKIITQIGVSPHSIVYMANC--NSETVAVKIIDININLNLDSLNTSLSLI---DHPNILR 88
           YK +T+IG      V++A    N++ VA+K I+IN N N   L T ++++    H NI+ 
Sbjct: 204 YKNMTKIGEGAAGEVFVATSSKNNKRVAIKKIEIN-NDNAKLLVTEIAIMKTSHHDNIVN 263

Query: 89  PHCCFIGEDGRIWIVMPFIPTGPLQSILSQIFPRGMPDHFISILLNQLLRALVYLHELGF 148
               +I  D  +W+ M F+  G L  IL       M +  I+ ++ + L+AL Y+H L  
Sbjct: 264 YIDSYIVNDRELWVAMEFMGGGCLTDILEAFDNIKMSEIQIAYVVKETLKALQYIHSLHR 323

Query: 149 VHKAIKPSNIFFDSQGSVKLSVFDIMISKNSTNEIDHD-SSIPYWVPPD----EEYSFKS 208
           +H+ IK  NI   S+GSVK++ F          +  +     PYW+ P+     +Y  K 
Sbjct: 324 IHRDIKSDNILLGSEGSVKIADFGYAAQLTQKQQKRNTVVGTPYWMAPELIRGHDYGVKV 383

Query: 209 DAWAVGVLAMEITWGGPPIIDPRILESQFFNISRRFPYEPKAERSRDRRLFGDSVKEFVK 268
           D W++G++ ME+  G PP +D   L + F   ++  P  P  E ++  + F D    F  
Sbjct: 384 DIWSLGIMMMEMAEGEPPYMDFPPLRALFLITTKGIP--PLKETTKWSKTFQD----FFS 443

Query: 269 FCLVKDQFGRPTALSLLEHPFV 281
            CL  +   RP A  LL+HPF+
Sbjct: 444 KCLDINVANRPDATDLLKHPFM 458

BLAST of Bhi12G001461 vs. ExPASy Swiss-Prot
Match: Q6P9R2 (Serine/threonine-protein kinase OSR1 OS=Mus musculus OX=10090 GN=Oxsr1 PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.4e-24
Identity = 84/345 (24.35%), Postives = 158/345 (45.80%), Query Frame = 0

Query: 29  YKIITQIGVSPHSIVYMANC--NSETVAVKIIDI-NINLNLDSL---NTSLSLIDHPNIL 88
           Y++   IG    ++V  A C    E VA+K I++     ++D L     ++S   HPNI+
Sbjct: 17  YELQEVIGSGATAVVQAAYCAPKKERVAIKRINLEKCQTSMDELLKEIQAMSQCHHPNIV 76

Query: 89  RPHCCFIGEDGRIWIVMPFIPTGPLQSILSQIFPRG------MPDHFISILLNQLLRALV 148
             +  F+ +D  +W+VM  +  G +  I+  I  +G      + +  I+ +L ++L  L 
Sbjct: 77  SYYTSFVVKD-ELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDEPTIATILREVLEGLE 136

Query: 149 YLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMISKNSTNEIDHDS------SIPYWVPP 208
           YLH+ G +H+ +K  NI     GSV+++ F +     +  +I  +         P W+ P
Sbjct: 137 YLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTPCWMAP 196

Query: 209 D-----EEYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNISRRFP-YEPKAERSR 268
           +       Y FK+D W+ G+ A+E+  G  P      ++     +    P  E   +   
Sbjct: 197 EVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQDKE 256

Query: 269 DRRLFGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGENSNFLFKCIPKGVIEIDKIF 328
             + +G S ++ +  CL KD   RPTA  LL H F +K +N  FL     + +++     
Sbjct: 257 MLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKFFQKAKNKEFL----QEKILQRAPTI 316

Query: 329 EQEKERIIGLMKNDEKLKNKSEGDKIMKISGWDYNNFLFELEPDE 350
            +  +++  +  +  +L    +G       GW++++  F+ E +E
Sbjct: 317 SERSKKVRRVPGSSGRLHKTEDG-------GWEWSDDEFDEESEE 349

BLAST of Bhi12G001461 vs. ExPASy Swiss-Prot
Match: Q5R495 (Serine/threonine-protein kinase OSR1 OS=Pongo abelii OX=9601 GN=OXSR1 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.4e-24
Identity = 84/345 (24.35%), Postives = 158/345 (45.80%), Query Frame = 0

Query: 29  YKIITQIGVSPHSIVYMANC--NSETVAVKIIDI-NINLNLDSL---NTSLSLIDHPNIL 88
           Y++   IG    ++V  A C    E VA+K I++     ++D L     ++S   HPNI+
Sbjct: 17  YELQEVIGSGATAVVQAAYCAPKKEKVAIKRINLEKCQTSMDELLKEIQAMSQCHHPNIV 76

Query: 89  RPHCCFIGEDGRIWIVMPFIPTGPLQSILSQIFPRG------MPDHFISILLNQLLRALV 148
             +  F+ +D  +W+VM  +  G +  I+  I  +G      + +  I+ +L ++L  L 
Sbjct: 77  SYYTSFVVKD-ELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDESTIATILREVLEGLE 136

Query: 149 YLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMISKNSTNEIDHDS------SIPYWVPP 208
           YLH+ G +H+ +K  NI     GSV+++ F +     +  +I  +         P W+ P
Sbjct: 137 YLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTPCWMAP 196

Query: 209 D-----EEYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNISRRFP-YEPKAERSR 268
           +       Y FK+D W+ G+ A+E+  G  P      ++     +    P  E   +   
Sbjct: 197 EVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQDKE 256

Query: 269 DRRLFGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGENSNFLFKCIPKGVIEIDKIF 328
             + +G S ++ +  CL KD   RPTA  LL H F +K +N  FL     + +++     
Sbjct: 257 MLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKFFQKAKNKEFL----QEKILQRAPTI 316

Query: 329 EQEKERIIGLMKNDEKLKNKSEGDKIMKISGWDYNNFLFELEPDE 350
            +  +++  +  +  +L    +G       GW++++  F+ E +E
Sbjct: 317 SERAKKVRRVPGSSGRLHKTEDG-------GWEWSDDEFDEESEE 349

BLAST of Bhi12G001461 vs. NCBI nr
Match: XP_038875030.1 (serine/threonine-protein kinase BLUS1-like [Benincasa hispida])

HSP 1 Score: 939.5 bits (2427), Expect = 1.3e-269
Identity = 489/530 (92.26%), Postives = 489/530 (92.26%), Query Frame = 0

Query: 1   MKQHQVQESATTTANSTLSFKFPTTSNGYKIITQIGVSPHSIVYMANCNSETVAVKIIDI 60
           MKQHQVQESATTTANSTLSFKFPTTSNGYKIITQIGVSPHSIVYMANCNSETVAVKIIDI
Sbjct: 1   MKQHQVQESATTTANSTLSFKFPTTSNGYKIITQIGVSPHSIVYMANCNSETVAVKIIDI 60

Query: 61  NINLNLDSLNTSLSLIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQSILSQIFPRGMP 120
           NINLNLDSLNTSLSLIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQSILSQIFPRGMP
Sbjct: 61  NINLNLDSLNTSLSLIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQSILSQIFPRGMP 120

Query: 121 DHFISILLNQLLRALVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMISKNSTNEIDH 180
           DHFISILLNQLLRALVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMISKNSTNEIDH
Sbjct: 121 DHFISILLNQLLRALVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMISKNSTNEIDH 180

Query: 181 DSSIPYWVPPDEEYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNISRRFPYEPKA 240
           DSSIPYWVPPDEEYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNISRRFPYEPKA
Sbjct: 181 DSSIPYWVPPDEEYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNISRRFPYEPKA 240

Query: 241 ERSRDRRLFGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGENSNFLFKCIPKGVIEI 300
           ERSRDRRLFGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGENSNFLFKCIPKGVIEI
Sbjct: 241 ERSRDRRLFGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGENSNFLFKCIPKGVIEI 300

Query: 301 DKIFEQEKERIIGLMKNDEKLKNKSEGDKIMKISGWDYNNFLFELEPDEESVGKRVRFGG 360
           DKIFEQEKERIIGLMKNDEKLKNKSEGDKIMKISGWDYNNFLFELEPDEESVGKRVRFGG
Sbjct: 301 DKIFEQEKERIIGLMKNDEKLKNKSEGDKIMKISGWDYNNFLFELEPDEESVGKRVRFGG 360

Query: 361 EVVRQFKKDEDEDKDEDEEEDEEEEEEESKKVDDMSPLLQKIKSNLSGVGESSKKAGEKK 420
           EV                                         SNLSGVGESSKKAGEKK
Sbjct: 361 EV-----------------------------------------SNLSGVGESSKKAGEKK 420

Query: 421 KKKKKKKKNVDEGSLVKKLCSLLDNIDEQREMVLVLINHIGYLTEKQLEMSNEIKKLEAD 480
           KKKKKKKKNVDEGSLVKKLCSLLDNIDEQREMVLVLINHIGYLTEKQLEMSNEIKKLEAD
Sbjct: 421 KKKKKKKKNVDEGSLVKKLCSLLDNIDEQREMVLVLINHIGYLTEKQLEMSNEIKKLEAD 480

Query: 481 LASEKERYFQLELECEFLKLKVPDSEDENEYSDEEQQYYGDEDEQPDSQE 531
           LASEKERYFQLELECEFLKLKVPDSEDENEYSDEEQQYYGDEDEQPDSQE
Sbjct: 481 LASEKERYFQLELECEFLKLKVPDSEDENEYSDEEQQYYGDEDEQPDSQE 489

BLAST of Bhi12G001461 vs. NCBI nr
Match: XP_008465200.1 (PREDICTED: serine/threonine-protein kinase BLUS1-like [Cucumis melo] >KAA0057526.1 serine/threonine-protein kinase BLUS1-like [Cucumis melo var. makuwa] >TYK08207.1 serine/threonine-protein kinase BLUS1-like [Cucumis melo var. makuwa])

HSP 1 Score: 634.8 bits (1636), Expect = 6.7e-178
Identity = 343/503 (68.19%), Postives = 401/503 (79.72%), Query Frame = 0

Query: 22  FPTTSNGYKIITQIGVSPHSIVYMANCNSE--------TVAVKIIDI-NINLNLDSLNTS 81
           FPTTS+ Y I+TQIG SP+S VY+A C+S+        TVA+KII+   I  + + LNTS
Sbjct: 3   FPTTSDQYTILTQIGASPNSAVYLAKCDSQDSSTSSTVTVAIKIIETPQIIFDTNLLNTS 62

Query: 82  LSLIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQSILSQIFPRGMPDHFISILLNQLL 141
           L+LIDHPNI+RPHCCF  +D R+WIVMP++  GPLQS+LS  FPRGMPD F SILL+Q+L
Sbjct: 63  LTLIDHPNIVRPHCCFFADDNRLWIVMPYMSAGPLQSVLSHSFPRGMPDQFTSILLSQVL 122

Query: 142 RALVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMISKNSTNEIDHDSSIPYWVPPDE 201
           +ALVYLH +GFVHKAIKPSNIFFDSQG+VKLSVFD+MIS  + N+ID DSSIPYWVPPD+
Sbjct: 123 QALVYLHRMGFVHKAIKPSNIFFDSQGTVKLSVFDLMIS--ADNKIDRDSSIPYWVPPDD 182

Query: 202 EYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNISRRFPYEPKAERSR----DRRL 261
           EYSFKSD W VGVLAME+TWGGPPIIDPRILESQ  NISRRFPYE K E  R    DRR+
Sbjct: 183 EYSFKSDIWEVGVLAMEVTWGGPPIIDPRILESQVLNISRRFPYELKTETGRRNSGDRRI 242

Query: 262 FGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGEN-SNFLFKCIPKGVIEIDKIFEQE 321
           FGD+VKEFVK CL+KD+  RPTA SLL+HPFV KGEN +NFLFK IPKGVIEID+ F+ E
Sbjct: 243 FGDAVKEFVKTCLLKDRLERPTAASLLDHPFVVKGENANNFLFKSIPKGVIEIDRRFQLE 302

Query: 322 KERIIGLMKNDEKLKNKSEGDKIMKISGWDYNNFLFELEPDEESVGKRVRFGGEVVRQFK 381
           KERIIG+M NDE LKN+SE  KI KISGW YN+FLFELEPD ES+GKRVRFGGE +R+F+
Sbjct: 303 KERIIGIMNNDEGLKNQSE--KITKISGWVYNDFLFELEPDGESLGKRVRFGGETIREFE 362

Query: 382 KDEDEDKDEDEEEDEEEEEEESKKVDDMSPLLQKIKSNLSGVGESSKKAGEKKKKKKKKK 441
           K          EE+EEEE E S KVDD   LL+ I+ NL   G+ S   GE  KKKKKKK
Sbjct: 363 K----------EEEEEEEGESSTKVDDTPSLLEIIEKNLMSGGDGS---GESSKKKKKKK 422

Query: 442 KNVDEGSLVKKLCSLLDNIDEQREMVLVLINHIGYLTEKQLEMSNEIKKLEADLASEKER 501
           K VD+ SLVK L S+L NID QREMV +LINH+GYL+EKQ EMSNEIK+LE +L +EKE+
Sbjct: 423 KIVDQESLVKMLTSVLGNIDAQREMVSMLINHVGYLSEKQSEMSNEIKRLETELKAEKEQ 482

Query: 502 YFQLELECEFLKLKVPDSEDENE 511
           YFQLELE EFLKLKVPDS+D+ +
Sbjct: 483 YFQLELEYEFLKLKVPDSDDDQK 488

BLAST of Bhi12G001461 vs. NCBI nr
Match: XP_022958864.1 (serine/threonine-protein kinase BLUS1-like [Cucurbita moschata])

HSP 1 Score: 490.3 bits (1261), Expect = 2.0e-134
Identity = 278/542 (51.29%), Postives = 372/542 (68.63%), Query Frame = 0

Query: 1   MKQHQVQESATTTANSTLSFKFPTTSNGYKIITQIGVSPHSIVYMANCNS----ETVAVK 60
           M + Q+Q+  T+ A  T S KFP  ++ Y+I +QIG S HS VY A C S      VA+K
Sbjct: 1   MDRRQIQDQETSAAKGTHS-KFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTIVAIK 60

Query: 61  IIDININLN---LDSLNTSLSLIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQSILSQ 120
           IID N+ ++   + ++NT+LS I+HP+IL PHCCF   DGR+WIVMPFIP GPLQ+I+SQ
Sbjct: 61  IIDSNVEIDQTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQAIISQ 120

Query: 121 IFPRGMPDHFISILLNQLLRALVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMISKN 180
            F  G+PD F+SIL+NQLLRALV+LHE G VHKA+K SNIFFD+ G V LS  D+ +SK 
Sbjct: 121 RFRTGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLS--DLDVSKE 180

Query: 181 STNEIDHDSSIPYWVPPDEEYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNISRR 240
              +    SS+PYW PP+E+YSFKSD W+VG+LAMEI +G PPI D +++++QF  ISRR
Sbjct: 181 MKKDC---SSVPYWDPPEEDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISRR 240

Query: 241 FPY----EPKAERSRDRRLFGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGENSNFL 300
           FPY    + ++ +S   R FGD++K FV  CL KD+F R +ALSLL+HPFVKKGENSNFL
Sbjct: 241 FPYDLNLDNRSGKSVVPRKFGDTLKHFVGSCLAKDRFARASALSLLDHPFVKKGENSNFL 300

Query: 301 FKCIPKGVIEIDKIFEQEKERIIGLMKNDEKLKNKSE-GDKIMKISGWDYNNFLFELEPD 360
           FK I KG++ I ++FEQ+K+RII LMKN+ +L   S+ G K+++I+GWDYNNFLFELEPD
Sbjct: 301 FKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNFLFELEPD 360

Query: 361 EESVGKRVRFGGEVVRQFKKDEDEDKDEDEEEDEEEEEEESKKVDDMSPLLQKIKSNLSG 420
           EES GKRVRF GE V  +K                 E+E+  K D        +KS + G
Sbjct: 361 EESFGKRVRFRGETVFSYK-----------------EQEQCNKFD--------MKSLMHG 420

Query: 421 VGESSKKAGEKKKKKKKKKKNVDEGSLVKKLCSLLDNIDEQREMVLVLINHIGYLTEKQL 480
                   G   +KKK+  + +D  +L   L SLLDNID Q+EMV +L+NH G++ +K L
Sbjct: 421 --------GSSDEKKKEVDQAMDTETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDL 480

Query: 481 EMSNEIKKLEADLASEKERYFQLELECEFLKLKVPDSEDENEYSDEEQQYYGDEDEQPDS 531
           EM  +IK+LE +LA+EKE+ FQLEL  E LKLK+PDS++E E  + E +   + ++QP+ 
Sbjct: 481 EMVKQIKRLEDELAAEKEKSFQLELNFELLKLKLPDSDEEQEQEEYEPEPEPEPEQQPER 503

BLAST of Bhi12G001461 vs. NCBI nr
Match: KAG6574930.1 (Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 487.6 bits (1254), Expect = 1.3e-133
Identity = 279/542 (51.48%), Postives = 372/542 (68.63%), Query Frame = 0

Query: 1   MKQHQVQESATTTANSTLSFKFPTTSNGYKIITQIGVSPHSIVYMANCNS----ETVAVK 60
           M + Q+Q+  T+ A  T S KFP  ++ Y+I +QIG S HS VY A C S      VA+K
Sbjct: 1   MDRRQIQDQETSAAKGTHS-KFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTIVAIK 60

Query: 61  IIDININLN---LDSLNTSLSLIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQSILSQ 120
           IID N+ ++   + ++NT+LS I+HP+IL PHCCF   DGR+WIVMPFIP GPLQ+I+SQ
Sbjct: 61  IIDSNVEIDQTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQAIISQ 120

Query: 121 IFPRGMPDHFISILLNQLLRALVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMISKN 180
            F  G+PD F+SIL+NQLLRALV+LHE G VHKA+K SNIFFD+ G V LS  D+ +SK 
Sbjct: 121 RFRTGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLS--DLDVSKE 180

Query: 181 STNEIDHDSSIPYWVPPDEEYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNISRR 240
              +    SS+PYW PP+E+YSFKSD W+VG+LAMEI +G PPI D +++++QF  ISRR
Sbjct: 181 MKKDC---SSVPYWDPPEEDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISRR 240

Query: 241 FPY----EPKAERSRDRRLFGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGENSNFL 300
           FPY    + ++ +S   R FGD++K FV  CL KD+F R +ALSLL+HPFVKKGENSNFL
Sbjct: 241 FPYDLNLDNRSGKSVVPRKFGDTLKHFVGSCLAKDRFARASALSLLDHPFVKKGENSNFL 300

Query: 301 FKCIPKGVIEIDKIFEQEKERIIGLMKNDEKLKNKSE-GDKIMKISGWDYNNFLFELEPD 360
           FK I KG++ I ++FEQ+K+RII LMKN+ +L   S+ G K+++I+GWDYNNFLFELEPD
Sbjct: 301 FKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNFLFELEPD 360

Query: 361 EESVGKRVRFGGEVVRQFKKDEDEDKDEDEEEDEEEEEEESKKVDDMSPLLQKIKSNLSG 420
           EES GKRVRF GE V  +K                 E+E+  K D        +KS + G
Sbjct: 361 EESFGKRVRFRGETVFSYK-----------------EQEQCNKFD--------MKSLMHG 420

Query: 421 VGESSKKAGEKKKKKKKKKKNVDEGSLVKKLCSLLDNIDEQREMVLVLINHIGYLTEKQL 480
                   G   +KKK+  + +D  +L   L SLLDNID Q+EMV +L+NH G++ +K L
Sbjct: 421 --------GSSDEKKKEVDQAMDTETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDL 480

Query: 481 EMSNEIKKLEADLASEKERYFQLELECEFLKLKVPDSEDENEYSDEEQQYYGDEDEQPDS 531
           EM  +IK+LE +LA+EKE+ FQLEL  E LKLK+PDS++E E   EE +   + ++QP+ 
Sbjct: 481 EMVKQIKRLEDELAAEKEKSFQLELNFELLKLKLPDSDEEQE--QEEYEPEPEPEQQPER 501

BLAST of Bhi12G001461 vs. NCBI nr
Match: KAG7013499.1 (Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 487.3 bits (1253), Expect = 1.7e-133
Identity = 279/547 (51.01%), Postives = 374/547 (68.37%), Query Frame = 0

Query: 1   MKQHQVQESATTTANSTLS-----FKFPTTSNGYKIITQIGVSPHSIVYMANCNS----E 60
           M + Q+Q+  T+ A+S  +      KFP  ++ Y+I +QIG S HS VY A C S     
Sbjct: 1   MDRRQIQDQETSAASSASAAKGTHSKFPKVADEYEIQSQIGASDHSTVYKAICKSADTPT 60

Query: 61  TVAVKIIDININLN---LDSLNTSLSLIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQ 120
            VA+KIID N+ ++   + ++NT+LS I+HP+IL PHCCF   DGR+WIVMPFIP GPLQ
Sbjct: 61  IVAIKIIDSNVEIDQTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQ 120

Query: 121 SILSQIFPRGMPDHFISILLNQLLRALVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDI 180
           +I+SQ F  G+PD F+SIL+NQLLRALV+LHE G VHKA+K SNIFFD+ G V LS  D+
Sbjct: 121 AIISQRFRTGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLS--DL 180

Query: 181 MISKNSTNEIDHDSSIPYWVPPDEEYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFF 240
            +SK    +    SS+PYW PP+E+YSFKSD W+VG+LAMEI +G PPI D +++++QF 
Sbjct: 181 DVSKEMKKDC---SSVPYWDPPEEDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFS 240

Query: 241 NISRRFPY----EPKAERSRDRRLFGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGE 300
            ISRRFPY    + ++ +S   R FGD++K FV  CL KD+F R +ALSLL+HPFVKKGE
Sbjct: 241 KISRRFPYDLNLDNRSGKSVVPRKFGDTLKHFVGSCLAKDRFARASALSLLDHPFVKKGE 300

Query: 301 NSNFLFKCIPKGVIEIDKIFEQEKERIIGLMKNDEKLKNKSE-GDKIMKISGWDYNNFLF 360
           NSNFLFK I KG++ I ++FEQ+K+RII LMKN+ +L   S+ G K+++I+GWDYNNFLF
Sbjct: 301 NSNFLFKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNFLF 360

Query: 361 ELEPDEESVGKRVRFGGEVVRQFKKDEDEDKDEDEEEDEEEEEEESKKVDDMSPLLQKIK 420
           ELEPDEES GKRVRF GE V  +K                 E+E+  K D        +K
Sbjct: 361 ELEPDEESFGKRVRFRGETVFSYK-----------------EQEQCNKFD--------MK 420

Query: 421 SNLSGVGESSKKAGEKKKKKKKKKKNVDEGSLVKKLCSLLDNIDEQREMVLVLINHIGYL 480
           S + G G S        +KKK+  + +D  +L   L SLLDNID Q+EMV +L+NH G++
Sbjct: 421 SLMHGGGSSD-------EKKKEVDQAMDTETLATLLSSLLDNIDTQKEMVSMLMNHCGFM 480

Query: 481 TEKQLEMSNEIKKLEADLASEKERYFQLELECEFLKLKVPDSEDENEYSDEEQQYYGDED 531
            +K LEM  +IK+LE +LA+EKE+ FQLEL  E LKLK+PDS++E E   EE +   + +
Sbjct: 481 HDKDLEMVKQIKRLEDELAAEKEKSFQLELNFELLKLKLPDSDEEQE--QEEYEPEPEPE 508

BLAST of Bhi12G001461 vs. ExPASy TrEMBL
Match: A0A5D3C8A9 (Serine/threonine-protein kinase BLUS1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1503G00250 PE=4 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 3.2e-178
Identity = 343/503 (68.19%), Postives = 401/503 (79.72%), Query Frame = 0

Query: 22  FPTTSNGYKIITQIGVSPHSIVYMANCNSE--------TVAVKIIDI-NINLNLDSLNTS 81
           FPTTS+ Y I+TQIG SP+S VY+A C+S+        TVA+KII+   I  + + LNTS
Sbjct: 3   FPTTSDQYTILTQIGASPNSAVYLAKCDSQDSSTSSTVTVAIKIIETPQIIFDTNLLNTS 62

Query: 82  LSLIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQSILSQIFPRGMPDHFISILLNQLL 141
           L+LIDHPNI+RPHCCF  +D R+WIVMP++  GPLQS+LS  FPRGMPD F SILL+Q+L
Sbjct: 63  LTLIDHPNIVRPHCCFFADDNRLWIVMPYMSAGPLQSVLSHSFPRGMPDQFTSILLSQVL 122

Query: 142 RALVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMISKNSTNEIDHDSSIPYWVPPDE 201
           +ALVYLH +GFVHKAIKPSNIFFDSQG+VKLSVFD+MIS  + N+ID DSSIPYWVPPD+
Sbjct: 123 QALVYLHRMGFVHKAIKPSNIFFDSQGTVKLSVFDLMIS--ADNKIDRDSSIPYWVPPDD 182

Query: 202 EYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNISRRFPYEPKAERSR----DRRL 261
           EYSFKSD W VGVLAME+TWGGPPIIDPRILESQ  NISRRFPYE K E  R    DRR+
Sbjct: 183 EYSFKSDIWEVGVLAMEVTWGGPPIIDPRILESQVLNISRRFPYELKTETGRRNSGDRRI 242

Query: 262 FGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGEN-SNFLFKCIPKGVIEIDKIFEQE 321
           FGD+VKEFVK CL+KD+  RPTA SLL+HPFV KGEN +NFLFK IPKGVIEID+ F+ E
Sbjct: 243 FGDAVKEFVKTCLLKDRLERPTAASLLDHPFVVKGENANNFLFKSIPKGVIEIDRRFQLE 302

Query: 322 KERIIGLMKNDEKLKNKSEGDKIMKISGWDYNNFLFELEPDEESVGKRVRFGGEVVRQFK 381
           KERIIG+M NDE LKN+SE  KI KISGW YN+FLFELEPD ES+GKRVRFGGE +R+F+
Sbjct: 303 KERIIGIMNNDEGLKNQSE--KITKISGWVYNDFLFELEPDGESLGKRVRFGGETIREFE 362

Query: 382 KDEDEDKDEDEEEDEEEEEEESKKVDDMSPLLQKIKSNLSGVGESSKKAGEKKKKKKKKK 441
           K          EE+EEEE E S KVDD   LL+ I+ NL   G+ S   GE  KKKKKKK
Sbjct: 363 K----------EEEEEEEGESSTKVDDTPSLLEIIEKNLMSGGDGS---GESSKKKKKKK 422

Query: 442 KNVDEGSLVKKLCSLLDNIDEQREMVLVLINHIGYLTEKQLEMSNEIKKLEADLASEKER 501
           K VD+ SLVK L S+L NID QREMV +LINH+GYL+EKQ EMSNEIK+LE +L +EKE+
Sbjct: 423 KIVDQESLVKMLTSVLGNIDAQREMVSMLINHVGYLSEKQSEMSNEIKRLETELKAEKEQ 482

Query: 502 YFQLELECEFLKLKVPDSEDENE 511
           YFQLELE EFLKLKVPDS+D+ +
Sbjct: 483 YFQLELEYEFLKLKVPDSDDDQK 488

BLAST of Bhi12G001461 vs. ExPASy TrEMBL
Match: A0A1S3CN88 (serine/threonine-protein kinase BLUS1-like OS=Cucumis melo OX=3656 GN=LOC103502862 PE=4 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 3.2e-178
Identity = 343/503 (68.19%), Postives = 401/503 (79.72%), Query Frame = 0

Query: 22  FPTTSNGYKIITQIGVSPHSIVYMANCNSE--------TVAVKIIDI-NINLNLDSLNTS 81
           FPTTS+ Y I+TQIG SP+S VY+A C+S+        TVA+KII+   I  + + LNTS
Sbjct: 3   FPTTSDQYTILTQIGASPNSAVYLAKCDSQDSSTSSTVTVAIKIIETPQIIFDTNLLNTS 62

Query: 82  LSLIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQSILSQIFPRGMPDHFISILLNQLL 141
           L+LIDHPNI+RPHCCF  +D R+WIVMP++  GPLQS+LS  FPRGMPD F SILL+Q+L
Sbjct: 63  LTLIDHPNIVRPHCCFFADDNRLWIVMPYMSAGPLQSVLSHSFPRGMPDQFTSILLSQVL 122

Query: 142 RALVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMISKNSTNEIDHDSSIPYWVPPDE 201
           +ALVYLH +GFVHKAIKPSNIFFDSQG+VKLSVFD+MIS  + N+ID DSSIPYWVPPD+
Sbjct: 123 QALVYLHRMGFVHKAIKPSNIFFDSQGTVKLSVFDLMIS--ADNKIDRDSSIPYWVPPDD 182

Query: 202 EYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNISRRFPYEPKAERSR----DRRL 261
           EYSFKSD W VGVLAME+TWGGPPIIDPRILESQ  NISRRFPYE K E  R    DRR+
Sbjct: 183 EYSFKSDIWEVGVLAMEVTWGGPPIIDPRILESQVLNISRRFPYELKTETGRRNSGDRRI 242

Query: 262 FGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGEN-SNFLFKCIPKGVIEIDKIFEQE 321
           FGD+VKEFVK CL+KD+  RPTA SLL+HPFV KGEN +NFLFK IPKGVIEID+ F+ E
Sbjct: 243 FGDAVKEFVKTCLLKDRLERPTAASLLDHPFVVKGENANNFLFKSIPKGVIEIDRRFQLE 302

Query: 322 KERIIGLMKNDEKLKNKSEGDKIMKISGWDYNNFLFELEPDEESVGKRVRFGGEVVRQFK 381
           KERIIG+M NDE LKN+SE  KI KISGW YN+FLFELEPD ES+GKRVRFGGE +R+F+
Sbjct: 303 KERIIGIMNNDEGLKNQSE--KITKISGWVYNDFLFELEPDGESLGKRVRFGGETIREFE 362

Query: 382 KDEDEDKDEDEEEDEEEEEEESKKVDDMSPLLQKIKSNLSGVGESSKKAGEKKKKKKKKK 441
           K          EE+EEEE E S KVDD   LL+ I+ NL   G+ S   GE  KKKKKKK
Sbjct: 363 K----------EEEEEEEGESSTKVDDTPSLLEIIEKNLMSGGDGS---GESSKKKKKKK 422

Query: 442 KNVDEGSLVKKLCSLLDNIDEQREMVLVLINHIGYLTEKQLEMSNEIKKLEADLASEKER 501
           K VD+ SLVK L S+L NID QREMV +LINH+GYL+EKQ EMSNEIK+LE +L +EKE+
Sbjct: 423 KIVDQESLVKMLTSVLGNIDAQREMVSMLINHVGYLSEKQSEMSNEIKRLETELKAEKEQ 482

Query: 502 YFQLELECEFLKLKVPDSEDENE 511
           YFQLELE EFLKLKVPDS+D+ +
Sbjct: 483 YFQLELEYEFLKLKVPDSDDDQK 488

BLAST of Bhi12G001461 vs. ExPASy TrEMBL
Match: A0A6J1H6C5 (serine/threonine-protein kinase BLUS1-like OS=Cucurbita moschata OX=3662 GN=LOC111460018 PE=4 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 9.8e-135
Identity = 278/542 (51.29%), Postives = 372/542 (68.63%), Query Frame = 0

Query: 1   MKQHQVQESATTTANSTLSFKFPTTSNGYKIITQIGVSPHSIVYMANCNS----ETVAVK 60
           M + Q+Q+  T+ A  T S KFP  ++ Y+I +QIG S HS VY A C S      VA+K
Sbjct: 1   MDRRQIQDQETSAAKGTHS-KFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTIVAIK 60

Query: 61  IIDININLN---LDSLNTSLSLIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQSILSQ 120
           IID N+ ++   + ++NT+LS I+HP+IL PHCCF   DGR+WIVMPFIP GPLQ+I+SQ
Sbjct: 61  IIDSNVEIDQTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQAIISQ 120

Query: 121 IFPRGMPDHFISILLNQLLRALVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMISKN 180
            F  G+PD F+SIL+NQLLRALV+LHE G VHKA+K SNIFFD+ G V LS  D+ +SK 
Sbjct: 121 RFRTGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLS--DLDVSKE 180

Query: 181 STNEIDHDSSIPYWVPPDEEYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNISRR 240
              +    SS+PYW PP+E+YSFKSD W+VG+LAMEI +G PPI D +++++QF  ISRR
Sbjct: 181 MKKDC---SSVPYWDPPEEDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISRR 240

Query: 241 FPY----EPKAERSRDRRLFGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGENSNFL 300
           FPY    + ++ +S   R FGD++K FV  CL KD+F R +ALSLL+HPFVKKGENSNFL
Sbjct: 241 FPYDLNLDNRSGKSVVPRKFGDTLKHFVGSCLAKDRFARASALSLLDHPFVKKGENSNFL 300

Query: 301 FKCIPKGVIEIDKIFEQEKERIIGLMKNDEKLKNKSE-GDKIMKISGWDYNNFLFELEPD 360
           FK I KG++ I ++FEQ+K+RII LMKN+ +L   S+ G K+++I+GWDYNNFLFELEPD
Sbjct: 301 FKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNFLFELEPD 360

Query: 361 EESVGKRVRFGGEVVRQFKKDEDEDKDEDEEEDEEEEEEESKKVDDMSPLLQKIKSNLSG 420
           EES GKRVRF GE V  +K                 E+E+  K D        +KS + G
Sbjct: 361 EESFGKRVRFRGETVFSYK-----------------EQEQCNKFD--------MKSLMHG 420

Query: 421 VGESSKKAGEKKKKKKKKKKNVDEGSLVKKLCSLLDNIDEQREMVLVLINHIGYLTEKQL 480
                   G   +KKK+  + +D  +L   L SLLDNID Q+EMV +L+NH G++ +K L
Sbjct: 421 --------GSSDEKKKEVDQAMDTETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDL 480

Query: 481 EMSNEIKKLEADLASEKERYFQLELECEFLKLKVPDSEDENEYSDEEQQYYGDEDEQPDS 531
           EM  +IK+LE +LA+EKE+ FQLEL  E LKLK+PDS++E E  + E +   + ++QP+ 
Sbjct: 481 EMVKQIKRLEDELAAEKEKSFQLELNFELLKLKLPDSDEEQEQEEYEPEPEPEPEQQPER 503

BLAST of Bhi12G001461 vs. ExPASy TrEMBL
Match: A0A6J1KYZ4 (serine/threonine-protein kinase BLUS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498878 PE=4 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 2.3e-131
Identity = 274/546 (50.18%), Postives = 370/546 (67.77%), Query Frame = 0

Query: 1   MKQHQVQESATTTANSTLSFKFPTTSNGYKIITQIGVSPHSIVYMANCNSE----TVAVK 60
           M + Q+Q+  T+ A  T  FKFP  ++ Y+I +QIG S HS VY A C S      VA+K
Sbjct: 1   MDRRQIQDQETSAAKGT-HFKFPKVADEYEIQSQIGASDHSTVYKAICKSADSTMIVAIK 60

Query: 61  IIDININLN---LDSLNTSLSLIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQSILSQ 120
           IID N+ ++   + ++NT+LS I+HP+IL PHCCFI  DG +WIVMPFI  GPLQ+I+SQ
Sbjct: 61  IIDSNVEIDPTAITNINTNLSFINHPHILSPHCCFIANDGCLWIVMPFIHAGPLQAIISQ 120

Query: 121 IFPRGMPDHFISILLNQLLRALVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMISKN 180
            F  G+PD F+SIL+NQLLRALV+LHE G VHK++K SNIFFD+ G V LS  D+ +SK+
Sbjct: 121 RFRTGLPDQFMSILINQLLRALVFLHEKGLVHKSVKASNIFFDTHGCVLLS--DLDVSKD 180

Query: 181 STNEIDHDSSIPYWVPPDEEYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNISRR 240
              +    SS+PYWVPP+E+YS KSD W+VG+LAMEI +G PPI D +++++QF  ISRR
Sbjct: 181 MNKDC---SSVPYWVPPEEDYSSKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISRR 240

Query: 241 FPY----EPKAERSRDRRLFGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGENSNFL 300
           FPY    + ++ +S   R FGD++K FV  CL KD F R +ALSLL+HPFVKKGENSNFL
Sbjct: 241 FPYDLNLDTRSGKSVVPRKFGDTLKHFVGSCLAKDPFARVSALSLLDHPFVKKGENSNFL 300

Query: 301 FKCIPKGVIEIDKIFEQEKERIIGLMKNDEKLKNKSE---GDKIMKISGWDYNNFLFELE 360
           FK I KG++ I ++FEQ+K+RII LMKN+ +L   S+   G K+++I+GWDYNNFLFELE
Sbjct: 301 FKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGGGKVLRITGWDYNNFLFELE 360

Query: 361 PDEESVGKRVRFGGEVVRQFKKDEDEDKDEDEEEDEEEEEEESKKVDDMSPLLQKIKSNL 420
           PDEES GKRVRF GE V  +K                 E+E+  K D        +KS +
Sbjct: 361 PDEESFGKRVRFRGETVFSYK-----------------EQEQCNKFD--------MKSLM 420

Query: 421 SGVGESSKKAGEKKKKKKKKKKNVDEGSLVKKLCSLLDNIDEQREMVLVLINHIGYLTEK 480
            G  +          +KK+  + +D  +L   L SLLDNID Q+EMV +L+NH G++ +K
Sbjct: 421 HGGSD----------EKKEVDQAMDSETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDK 480

Query: 481 QLEMSNEIKKLEADLASEKERYFQLELECEFLKLKVPDSEDENEYS--DEEQQYYGDEDE 531
            LEM  +IK+LE +LA+EKE+ FQLEL  E LKLK+PDS++E E    + E +   + ++
Sbjct: 481 DLEMVKQIKRLEDELAAEKEKSFQLELNFELLKLKLPDSDEEQEQEEYEPEPEPEPEPEQ 505

BLAST of Bhi12G001461 vs. ExPASy TrEMBL
Match: A0A6J1CA92 (serine/threonine-protein kinase BLUS1-like OS=Momordica charantia OX=3673 GN=LOC111009741 PE=4 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 6.0e-124
Identity = 261/517 (50.48%), Postives = 354/517 (68.47%), Query Frame = 0

Query: 5   QVQESATTTANSTLSFKFPTTSNGYKIITQIGVSPHSIVYMANC----NSETVAVKIIDI 64
           Q Q+     A   L F+FPT ++GYKI+ QIGVS HS+VY A C    NS  VAVK+ID+
Sbjct: 6   QDQDPENAAAAGRLHFQFPTAADGYKILYQIGVSDHSVVYKAICESANNSAKVAVKVIDV 65

Query: 65  NINLN---LDSLNTSLSLIDHPNILRPHCCFIGEDGRIWIVMPFIPTGPLQSILSQIFPR 124
           + +++   +  +N SLSLIDH NIL PHCCF   D R+W VMPFI  GPLQ+IL+  FP 
Sbjct: 66  DRHVDEFFVMKMN-SLSLIDHANILSPHCCFT-VDRRLWTVMPFIHAGPLQAILANRFPN 125

Query: 125 GMPDHFISILLNQLLRALVYLHELGFVHKAIKPSNIFFDSQGSVKLSVFDIMISKNSTNE 184
           G+PD F++ILL+QLLR+L+ LHELG+VH+ IK SNIF DS+GSVKLS  ++ +     ++
Sbjct: 126 GLPDQFLAILLHQLLRSLICLHELGYVHRNIKASNIFVDSEGSVKLSDLNVYVG----DD 185

Query: 185 IDHDSSIPYWVPPDEEYSFKSDAWAVGVLAMEITWGGPPIIDPRILESQFFNISRRFPYE 244
            D DSS+PYW PPDEEYSFKSD WA+G++A+E+ +G PPI D R + SQ  NISRRFPY 
Sbjct: 186 DDDDSSVPYWDPPDEEYSFKSDVWAIGIVALEVAYGCPPISDSRTIRSQLLNISRRFPYN 245

Query: 245 PKAERSRDR----RLFGDSVKEFVKFCLVKDQFGRPTALSLLEHPFVKKGENSNFLFKCI 304
              ++   +    R FG+  K+FV  CLVKD   RP+A SLL HPF+KKGE SN LFK I
Sbjct: 246 LNLDKMSSKSVIDRKFGELFKDFVSSCLVKDPSKRPSAASLLAHPFIKKGETSNVLFKSI 305

Query: 305 PKGVIEIDKIFEQEKERIIGLMKNDEKLKNKSEGDKIMKISGWDYNNFLFELEPDEESVG 364
            K +  I+  FE+ K+R++ LMK D ++++ + G+ ++K+SGWD+NNFLFELEPDEES+G
Sbjct: 306 AKDMAGIEDRFEKNKDRLVELMKKDRRVEDNNRGN-VVKVSGWDFNNFLFELEPDEESLG 365

Query: 365 KRVRFGGEVVRQFKKDEDEDKDEDEEEDEEEEEEESKKVDDMSPLLQKIKSNLSGVGESS 424
           KRVRF GE +   +         D+     E  E SK      PLL K   +LS  G S 
Sbjct: 366 KRVRFRGETIIPLR---------DDHVSLNEAGEASK---FNVPLLLKRMDSLSLSGLS- 425

Query: 425 KKAGEKKKKKKKKKKNVDEGSLVKKLCSLLDNIDEQREMVLVLINHIGYLTEKQLEMSNE 484
              G    + +++ + +D  +L K L SL+++ID+QRE+++++I+  G L E+ +EM+ E
Sbjct: 426 ---GRMGSRAEEEVRVMDSETLAKVLTSLVESIDQQREVIMMVISQFGRLLERDMEMTKE 485

Query: 485 IKKLEADLASEKERYFQLELECEFLKLKVPDSEDENE 511
           IKKL+A+LA EKER FQLEL+ E LKLK+PDSEDE++
Sbjct: 486 IKKLKAELAYEKERSFQLELDYEVLKLKLPDSEDEDK 499

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G14480.11.7e-4629.26Protein kinase superfamily protein [more]
AT1G23700.14.1e-4033.03Protein kinase superfamily protein [more]
AT1G79640.13.2e-3728.70Protein kinase superfamily protein [more]
AT1G79640.23.2e-3728.70Protein kinase superfamily protein [more]
AT1G70430.15.5e-3731.55Protein kinase superfamily protein [more]
Match NameE-valueIdentityDescription
O233042.4e-4529.26Serine/threonine-protein kinase BLUS1 OS=Arabidopsis thaliana OX=3702 GN=BLUS1 P... [more]
Q551H48.0e-3327.61Serine/threonine-protein kinase fray2 OS=Dictyostelium discoideum OX=44689 GN=fr... [more]
Q55GV36.1e-2531.30Serine/threonine-protein kinase pakC OS=Dictyostelium discoideum OX=44689 GN=pak... [more]
Q6P9R21.4e-2424.35Serine/threonine-protein kinase OSR1 OS=Mus musculus OX=10090 GN=Oxsr1 PE=1 SV=1[more]
Q5R4951.4e-2424.35Serine/threonine-protein kinase OSR1 OS=Pongo abelii OX=9601 GN=OXSR1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038875030.11.3e-26992.26serine/threonine-protein kinase BLUS1-like [Benincasa hispida][more]
XP_008465200.16.7e-17868.19PREDICTED: serine/threonine-protein kinase BLUS1-like [Cucumis melo] >KAA0057526... [more]
XP_022958864.12.0e-13451.29serine/threonine-protein kinase BLUS1-like [Cucurbita moschata][more]
KAG6574930.11.3e-13351.48Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. so... [more]
KAG7013499.11.7e-13351.01Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. ar... [more]
Match NameE-valueIdentityDescription
A0A5D3C8A93.2e-17868.19Serine/threonine-protein kinase BLUS1-like OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3CN883.2e-17868.19serine/threonine-protein kinase BLUS1-like OS=Cucumis melo OX=3656 GN=LOC1035028... [more]
A0A6J1H6C59.8e-13551.29serine/threonine-protein kinase BLUS1-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1KYZ42.3e-13150.18serine/threonine-protein kinase BLUS1-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1CA926.0e-12450.48serine/threonine-protein kinase BLUS1-like OS=Momordica charantia OX=3673 GN=LOC... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 367..388
NoneNo IPR availableCOILSCoilCoilcoord: 467..494
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 25..317
e-value: 1.4E-44
score: 154.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 372..390
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 367..429
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 507..530
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 504..530
NoneNo IPR availablePANTHERPTHR48014:SF7SERINE/THREONINE-PROTEIN KINASE BLUS1coord: 17..508
NoneNo IPR availablePANTHERPTHR48014SERINE/THREONINE-PROTEIN KINASE FRAY2coord: 17..508
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 29..280
e-value: 3.9E-28
score: 98.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 29..280
score: 29.926638
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 24..293

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi12M001461Bhi12M001461mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity