Bhi11G002328 (gene) Wax gourd (B227) v1

Overview


Name	Bhi11G002328
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	3'-5' exonuclease domain-containing protein
Location	chr11: 75293041 .. 75293673 (-)
RNA-Seq Expression	Bhi11G002328
Synteny	Bhi11G002328

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCATTCTGAGCATCTCCGACCGCCAAATCCCCCTCAACTCCCACGACTACTACGATATCACTTTCGACAACGATGCTATCCTGACTCTCCTTACTGCCTGTCTTTCCGTGGTCGACATGTGGATCTCTGAAATCCTCCGCATCCACCGCCGCAGACTCCACCGTCTCGTCGTCGGGCTTGACATTGAGTGGCGCCCTAACTTCAATCGTCGTTACCGTAAACCCGTTGCCACTTTGCAACTCTGCGTTGGTCGCCGCTGCCTCATCTTCCAACTCATCCACACCTCCGAAATACCTGAGTCTTTGATCGATTTCTTGGAGGACGATACCTTCACTTTCGTCGGCGTCGGAATCGATCAGGACGTTCAACAGCTCTACAATGACTACGATCTGAAAGTCGCTAACACTGTCGATCTTAGGGAGTTGGCTGCCAATGAGATGGAGAGAGATGATTTGAAGAATGCGGGACTGAAGATTTTGGGAAGAGAGGTTCTCGGAAGGGAGATTGATAAGCCGAGAAATGTGACGCTGAGTCAGTGGGATCGTCAATGTCTGAGTCCTGGTCAGGTACTCTATGCTACTGTTGATGCTTTTTTGTCGTTTGAGATTGGAAGGTATCTGCTGAGTTAA

mRNA sequence

ATGGCCATTCTGAGCATCTCCGACCGCCAAATCCCCCTCAACTCCCACGACTACTACGATATCACTTTCGACAACGATGCTATCCTGACTCTCCTTACTGCCTGTCTTTCCGTGGTCGACATGTGGATCTCTGAAATCCTCCGCATCCACCGCCGCAGACTCCACCGTCTCGTCGTCGGGCTTGACATTGAGTGGCGCCCTAACTTCAATCGTCGTTACCGTAAACCCGTTGCCACTTTGCAACTCTGCGTTGGTCGCCGCTGCCTCATCTTCCAACTCATCCACACCTCCGAAATACCTGAGTCTTTGATCGATTTCTTGGAGGACGATACCTTCACTTTCGTCGGCGTCGGAATCGATCAGGACGTTCAACAGCTCTACAATGACTACGATCTGAAAGTCGCTAACACTGTCGATCTTAGGGAGTTGGCTGCCAATGAGATGGAGAGAGATGATTTGAAGAATGCGGGACTGAAGATTTTGGGAAGAGAGGTTCTCGGAAGGGAGATTGATAAGCCGAGAAATGTGACGCTGAGTCAGTGGGATCGTCAATGTCTGAGTCCTGGTCAGGTACTCTATGCTACTGTTGATGCTTTTTTGTCGTTTGAGATTGGAAGGTATCTGCTGAGTTAA

Coding sequence (CDS)

ATGGCCATTCTGAGCATCTCCGACCGCCAAATCCCCCTCAACTCCCACGACTACTACGATATCACTTTCGACAACGATGCTATCCTGACTCTCCTTACTGCCTGTCTTTCCGTGGTCGACATGTGGATCTCTGAAATCCTCCGCATCCACCGCCGCAGACTCCACCGTCTCGTCGTCGGGCTTGACATTGAGTGGCGCCCTAACTTCAATCGTCGTTACCGTAAACCCGTTGCCACTTTGCAACTCTGCGTTGGTCGCCGCTGCCTCATCTTCCAACTCATCCACACCTCCGAAATACCTGAGTCTTTGATCGATTTCTTGGAGGACGATACCTTCACTTTCGTCGGCGTCGGAATCGATCAGGACGTTCAACAGCTCTACAATGACTACGATCTGAAAGTCGCTAACACTGTCGATCTTAGGGAGTTGGCTGCCAATGAGATGGAGAGAGATGATTTGAAGAATGCGGGACTGAAGATTTTGGGAAGAGAGGTTCTCGGAAGGGAGATTGATAAGCCGAGAAATGTGACGCTGAGTCAGTGGGATCGTCAATGTCTGAGTCCTGGTCAGGTACTCTATGCTACTGTTGATGCTTTTTTGTCGTTTGAGATTGGAAGGTATCTGCTGAGTTAA

Protein sequence

MAILSISDRQIPLNSHDYYDITFDNDAILTLLTACLSVVDMWISEILRIHRRRLHRLVVGLDIEWRPNFNRRYRKPVATLQLCVGRRCLIFQLIHTSEIPESLIDFLEDDTFTFVGVGIDQDVQQLYNDYDLKVANTVDLRELAANEMERDDLKNAGLKILGREVLGREIDKPRNVTLSQWDRQCLSPGQVLYATVDAFLSFEIGRYLLS

Homology

[Close]

BLAST of Bhi11G002328 vs. TAIR 10
Match: AT2G36110.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 104.0 bits (258), Expect = 1.5e-22
Identity = 67/193 (34.72%), Postives = 99/193 (51.30%), Query Frame = 0


Query: 16  HDYYDITFDNDAILTLLTACLSVVDMWISEILRIHR-RRLHRLVVGLDIEWRPNFNRRYR 75
           H+   I F  + ++  +T   S +  WI  I    R R  H LVVGLD++W P  +    
Sbjct: 14  HENRYIDFFGERLIVTVTHTTSTIRRWIHSIRFFSRLRSSHPLVVGLDVQWTPGGS---D 73

Query: 76  KPVATLQLCVGRRCLIFQLIHTSEIPESLIDFLEDDTFTFVGVGIDQDVQQLYN-DYDLK 135
            P   LQLCVG RCLI QL H   IPE L  FLED+T TFVGV   QD  +L    + L+
Sbjct: 74  PPPDILQLCVGNRCLIIQLSHCKRIPEVLRSFLEDETITFVGVWNSQDQGKLERFRHQLE 133

Query: 136 VANTVDLRELAANEMERDDLKNAGLKILGREVLG-REIDKPRNVTLSQWDRQCLSPGQVL 195
           +   +D+R           L N+  + +  E LG + + K + + +S W  + LS  Q++
Sbjct: 134 IWRLLDIRHYLPTR-----LLNSSFEKIVEECLGYKGVRKDKEICMSNWGARSLSHDQIV 193

Query: 196 YATVDAFLSFEIG 206
            A+ D ++  ++G
Sbjct: 194 QASDDVYVCCKLG 198

[Close]

BLAST of Bhi11G002328 vs. TAIR 10
Match: AT4G13870.2 (Werner syndrome-like exonuclease )

HSP 1 Score: 98.6 bits (244), Expect = 6.3e-21
Identity = 61/170 (35.88%), Postives = 93/170 (54.71%), Query Frame = 0


Query: 41  MWISEILRIHRRRLHRLVVGLDIEWRPNFNRRYRK-PVATLQLCV-GRRCLIFQLIHTSE 100
           M + ++L   R       VGLDIEWRP+F +      VAT+Q+CV    C +  + H S 
Sbjct: 112 MQLIKVLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFH-SG 171

Query: 101 IPESLIDFLEDDTFTFVGVGIDQDVQQLYNDYDLKVANTVDLRELAANEMERDDLKNAGL 160
           IP+SL   +ED T   VG+GID D  +L++DY + + +  DL +LA  ++  D  K  GL
Sbjct: 172 IPQSLQHLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGL 231

Query: 161 KILGREVLGREIDKPRNVTLSQWDRQCLSPGQVLYATVDAFLSFEIGRYL 209
             L   ++ +E+ KP  + L  W+   LS  Q+ YA  DA+ S+ + + L
Sbjct: 232 ASLTETLVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVL 278

[Close]

BLAST of Bhi11G002328 vs. TAIR 10
Match: AT3G12430.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 98.2 bits (243), Expect = 8.3e-21
Identity = 63/209 (30.14%), Postives = 102/209 (48.80%), Query Frame = 0


Query: 19  YDITFDNDAILTLLTACLSVVDMWISEILRIHRRRLHRLVVGLDIEWRPN---------- 78
           Y + F  +  + ++T   SV+  WI ++L  +R   H LVVG+ ++W P+          
Sbjct: 17  YLVDFFGEEFIVIVTPDPSVIGQWIHDVLSHNRFSSHPLVVGVGVQWTPSSYYSASSPVR 76

Query: 79  ----------------FNRRYRKPVATLQLCVGRRCLIFQLIHTSEIPESLIDFLEDDTF 138
                             RR+  P  TLQLCVG RC+I QL H   +P+ L +FL D  +
Sbjct: 77  YRSDSPPVRYRSDSPRVRRRFDPPADTLQLCVGNRCIIIQLSHCERVPQVLRNFLADRDY 136

Query: 139 TFVGVGIDQDVQQL-YNDYDLKVANTVDLRELAANEMERDDLKNAGLKILGREVLGRE-I 198
           TFVG+   QD  +L  + ++L++A  +DLR+  ++   R  +K    + +  E LG   +
Sbjct: 137 TFVGIWNSQDAGKLKRSKHELEIAVLLDLRKFVSDSSGR-TMKRCSFEKIVEENLGHSGV 196

Query: 199 DKPRNVTLSQWDRQCLSPGQVLYATVDAF 200
              R V+ S W    L   Q+L A++D +
Sbjct: 197 RLDRKVSRSDWRVYDLRYVQILQASIDVY 224

[Close]

BLAST of Bhi11G002328 vs. TAIR 10
Match: AT4G13870.1 (Werner syndrome-like exonuclease )

HSP 1 Score: 97.8 bits (242), Expect = 1.1e-20
Identity = 60/166 (36.14%), Postives = 91/166 (54.82%), Query Frame = 0


Query: 41  MWISEILRIHRRRLHRLVVGLDIEWRPNFNRRYRK-PVATLQLCV-GRRCLIFQLIHTSE 100
           M + ++L   R       VGLDIEWRP+F +      VAT+Q+CV    C +  + H S 
Sbjct: 112 MQLIKVLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFH-SG 171

Query: 101 IPESLIDFLEDDTFTFVGVGIDQDVQQLYNDYDLKVANTVDLRELAANEMERDDLKNAGL 160
           IP+SL   +ED T   VG+GID D  +L++DY + + +  DL +LA  ++  D  K  GL
Sbjct: 172 IPQSLQHLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGL 231

Query: 161 KILGREVLGREIDKPRNVTLSQWDRQCLSPGQVLYATVDAFLSFEI 205
             L   ++ +E+ KP  + L  W+   LS  Q+ YA  DA+ S+ +
Sbjct: 232 ASLTETLVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHL 274

[Close]

BLAST of Bhi11G002328 vs. TAIR 10
Match: AT3G12460.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 3.1e-20
Identity = 63/209 (30.14%), Postives = 99/209 (47.37%), Query Frame = 0


Query: 19  YDITFDNDAILTLLTACLSVVDMWISEILRIHRRRLHRLVVGLDIEWRPNFNRRYRKPVA 78
           Y + F  + +   +T   SV+  WI ++   +R   H LVVG+ ++W P+       PV+
Sbjct: 17  YSVDFFGEELFVTVTPDPSVIGQWIHDVFFHNRLSSHPLVVGVGVQWTPSGYHPASPPVS 76

Query: 79  --------------------------TLQLCVGRRCLIFQLIHTSEIPESLIDFLEDDTF 138
                                     TLQLCVG RC+I QL +   +P+ L +FL D   
Sbjct: 77  YRSDSSPDSYRSDSPPVFYSSDPPADTLQLCVGNRCIIIQLRYCERVPQVLRNFLADRDN 136

Query: 139 TFVGVGIDQDVQQLYND-YDLKVANTVDLRELAANEMERDDLKNAGLKILGREVLGRE-I 198
           TFVG+   QD  +L    + L++A  +DLRE  ++   R  + N  L+ +  E LG   +
Sbjct: 137 TFVGIWNSQDAGKLERSRHQLEIAELMDLREFVSDSSGRRSMYNYSLEKIVEENLGYPGV 196

Query: 199 DKPRNVTLSQWDRQCLSPGQVLYATVDAF 200
              R V++S W    LS  Q+L A++D +
Sbjct: 197 RLDREVSMSDWRVYNLSYDQILQASIDVY 225

[Close]

BLAST of Bhi11G002328 vs. ExPASy Swiss-Prot
Match: Q84LH3 (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 8.9e-20
Identity = 61/170 (35.88%), Postives = 93/170 (54.71%), Query Frame = 0


Query: 41  MWISEILRIHRRRLHRLVVGLDIEWRPNFNRRYRK-PVATLQLCV-GRRCLIFQLIHTSE 100
           M + ++L   R       VGLDIEWRP+F +      VAT+Q+CV    C +  + H S 
Sbjct: 112 MQLIKVLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFH-SG 171

Query: 101 IPESLIDFLEDDTFTFVGVGIDQDVQQLYNDYDLKVANTVDLRELAANEMERDDLKNAGL 160
           IP+SL   +ED T   VG+GID D  +L++DY + + +  DL +LA  ++  D  K  GL
Sbjct: 172 IPQSLQHLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGL 231

Query: 161 KILGREVLGREIDKPRNVTLSQWDRQCLSPGQVLYATVDAFLSFEIGRYL 209
             L   ++ +E+ KP  + L  W+   LS  Q+ YA  DA+ S+ + + L
Sbjct: 232 ASLTETLVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVL 278

[Close]

BLAST of Bhi11G002328 vs. ExPASy Swiss-Prot
Match: Q14191 (Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2)

HSP 1 Score: 93.2 bits (230), Expect = 3.8e-18
Identity = 53/152 (34.87%), Postives = 84/152 (55.26%), Query Frame = 0


Query: 58  VVGLDIEWRPNFNRRYRKPVATLQLCVGR-RCLIFQLIHTSEIPESLIDFLEDDTFTFVG 117
           VVG D+EW P +NR     VA +QLCV   +C +F +   S  P+ L   LE+      G
Sbjct: 78  VVGFDMEWPPLYNRGKLGKVALIQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAG 137

Query: 118 VGIDQDVQQLYNDYDLKVANTVDLRELAANEMERDDLKNAGLKILGREVLGREIDKPRNV 177
           VGI+ D  +L  D+D+K+ N V+L ++A  +++  +     L  L + +LG+++ K +++
Sbjct: 138 VGIEGDQWKLLRDFDIKLKNFVELTDVANKKLKCTE--TWSLNSLVKHLLGKQLLKDKSI 197

Query: 178 TLSQWDRQCLSPGQVLYATVDAFLSFEIGRYL 209
             S W +  L+  Q LYA  DA+  F I R L
Sbjct: 198 RCSNWSKFPLTEDQKLYAATDAYAGFIIYRNL 227

[Close]

BLAST of Bhi11G002328 vs. ExPASy Swiss-Prot
Match: O09053 (Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn PE=1 SV=3)

HSP 1 Score: 85.9 bits (211), Expect = 6.0e-16
Identity = 48/143 (33.57%), Postives = 79/143 (55.24%), Query Frame = 0


Query: 58  VVGLDIEWRPNFNRRYRKPVATLQLCVGR-RCLIFQLIHTSEIPESLIDFLEDDTFTFVG 117
           VVG D+EW P +    R  VA +QLCV   +C +F +   S  P+ L   LE+ +    G
Sbjct: 72  VVGFDMEWPPIYKPGKRSRVAVIQLCVSESKCYLFHISSMSVFPQGLKMLLENKSIKKAG 131

Query: 118 VGIDQDVQQLYNDYDLKVANTVDLRELAANEMERDDLKNAGLKILGREVLGREIDKPRNV 177
           VGI+ D  +L  D+D+K+ + V+L ++A  +++    +   L  L + VLG+++ K +++
Sbjct: 132 VGIEGDQWKLLRDFDVKLESFVELTDVANEKLK--CAETWSLNGLVKHVLGKQLLKDKSI 191

Query: 178 TLSQWDRQCLSPGQVLYATVDAF 200
             S W    L+  Q LYA  DA+
Sbjct: 192 RCSNWSNFPLTEDQKLYAATDAY 212

[Close]

BLAST of Bhi11G002328 vs. ExPASy Swiss-Prot
Match: Q9VGN7 (Exonuclease 3'-5' domain-containing protein 2 OS=Drosophila melanogaster OX=7227 GN=Exd2 PE=2 SV=2)

HSP 1 Score: 85.5 bits (210), Expect = 7.8e-16
Identity = 57/164 (34.76%), Postives = 84/164 (51.22%), Query Frame = 0


Query: 42  WISEILRIHRRRLHRLVVGLDIEWRPNFNRRYRKPVATLQLCVGR-RCLIFQLIHTSEIP 101
           W+   L+ H +     V+G D EW        R+PVA LQL   R  C +F+L H  +IP
Sbjct: 65  WVLNELKNHCQTFK--VLGFDCEWITVGGS--RRPVALLQLSSHRGLCALFRLCHMKQIP 124

Query: 102 ESLIDFLEDDTFTFVGVGIDQDVQQLYNDYDLKVANTVDLRELAANEMERDDLKNAGLKI 161
           + L + LEDD+   VGV   +D  +L +DY + VA+T+DLR L      + +    GL  
Sbjct: 125 QDLRELLEDDSVIKVGVAPQEDAMKLSHDYGVGVASTLDLRFLCVMAGHKPE----GLGK 184

Query: 162 LGREVLGREIDKPRNVTLSQWDRQCLSPGQVLYATVDAFLSFEI 205
           L +  L   +DK   +  S W+ + L P Q+ YA  DA ++  I
Sbjct: 185 LSKTHLNYTLDKHWRLACSNWEAKTLEPKQLDYAANDALMAVAI 220

[Close]

BLAST of Bhi11G002328 vs. ExPASy Swiss-Prot
Match: O93530 (Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.5e-14
Identity = 50/154 (32.47%), Postives = 83/154 (53.90%), Query Frame = 0


Query: 58  VVGLDIEWRPNFNRRYRKPVATLQLCVG-RRCLIFQLIHTSEIPESLIDFLEDDTFTFVG 117
           V+G DIEW P + +     VA +Q+CV  ++C +F +   +  P+ L   LED++   VG
Sbjct: 72  VLGFDIEWPPVYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLKRLLEDESVRKVG 131

Query: 118 VGIDQDVQQLYNDYDLKVANTVDLRELAANEMERDD--LKNAGLKILGREVLGREIDKPR 177
           VGI+ D  +L +DY+LK+   ++L E+A  ++   +    N  +K L +E    ++ K +
Sbjct: 132 VGIEGDQWKLMSDYELKLKGFIELSEMANQKLRCKEKWTFNGLIKHLFKE----QLYKRK 191

Query: 178 NVTLSQWDRQCLSPGQVLYATVDAFLSFEIGRYL 209
           +   S WD   L+  Q LYA  DA+    I + L
Sbjct: 192 SYRCSNWDIFLLTEDQKLYAATDAYAGLLIYKKL 221

[Close]

BLAST of Bhi11G002328 vs. NCBI nr
Match: XP_038906336.1 (Werner Syndrome-like exonuclease [Benincasa hispida])

HSP 1 Score: 422.9 bits (1086), Expect = 1.6e-114
Identity = 210/210 (100.00%), Postives = 210/210 (100.00%), Query Frame = 0


Query: 1   MAILSISDRQIPLNSHDYYDITFDNDAILTLLTACLSVVDMWISEILRIHRRRLHRLVVG 60
           MAILSISDRQIPLNSHDYYDITFDNDAILTLLTACLSVVDMWISEILRIHRRRLHRLVVG
Sbjct: 1   MAILSISDRQIPLNSHDYYDITFDNDAILTLLTACLSVVDMWISEILRIHRRRLHRLVVG 60

Query: 61  LDIEWRPNFNRRYRKPVATLQLCVGRRCLIFQLIHTSEIPESLIDFLEDDTFTFVGVGID 120
           LDIEWRPNFNRRYRKPVATLQLCVGRRCLIFQLIHTSEIPESLIDFLEDDTFTFVGVGID
Sbjct: 61  LDIEWRPNFNRRYRKPVATLQLCVGRRCLIFQLIHTSEIPESLIDFLEDDTFTFVGVGID 120

Query: 121 QDVQQLYNDYDLKVANTVDLRELAANEMERDDLKNAGLKILGREVLGREIDKPRNVTLSQ 180
           QDVQQLYNDYDLKVANTVDLRELAANEMERDDLKNAGLKILGREVLGREIDKPRNVTLSQ
Sbjct: 121 QDVQQLYNDYDLKVANTVDLRELAANEMERDDLKNAGLKILGREVLGREIDKPRNVTLSQ 180

Query: 181 WDRQCLSPGQVLYATVDAFLSFEIGRYLLS 211
           WDRQCLSPGQVLYATVDAFLSFEIGRYLLS
Sbjct: 181 WDRQCLSPGQVLYATVDAFLSFEIGRYLLS 210

[Close]

BLAST of Bhi11G002328 vs. NCBI nr
Match: XP_004136771.1 (Werner Syndrome-like exonuclease [Cucumis sativus])

HSP 1 Score: 360.5 bits (924), Expect = 9.6e-96
Identity = 176/210 (83.81%), Postives = 192/210 (91.43%), Query Frame = 0


Query: 1   MAILSISDRQIPLNSHDYYDITFDNDAILTLLTACLSVVDMWISEILRIHRRRLHRLVVG 60
           MA L+ISDRQIPLNSHDYYDITFD+D ILTL+TA  S+VDMWISEILRIHRRRLHRLVVG
Sbjct: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60

Query: 61  LDIEWRPNFNRRYRKPVATLQLCVGRRCLIFQLIHTSEIPESLIDFLEDDTFTFVGVGID 120
           LD+EWRPNF+R +R PVATLQLCVGRRCLIFQLIH SE P+SLIDFLEDDTFTFVGVGID
Sbjct: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120

Query: 121 QDVQQLYNDYDLKVANTVDLRELAANEMERDDLKNAGLKILGREVLGREIDKPRNVTLSQ 180
            DV +LYNDYDL VANTVDLRELAA+EM+ D+ + AGLK LGREVLGREIDKPRNV LS+
Sbjct: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180

Query: 181 WDRQCLSPGQVLYATVDAFLSFEIGRYLLS 211
           WDRQ L+P Q+LYATVDAFLSFEIGRYLLS
Sbjct: 181 WDRQWLNPAQILYATVDAFLSFEIGRYLLS 210

[Close]

BLAST of Bhi11G002328 vs. NCBI nr
Match: KAE8651170.1 (hypothetical protein Csa_000755 [Cucumis sativus])

HSP 1 Score: 353.6 bits (906), Expect = 1.2e-93
Identity = 172/206 (83.50%), Postives = 188/206 (91.26%), Query Frame = 0


Query: 1   MAILSISDRQIPLNSHDYYDITFDNDAILTLLTACLSVVDMWISEILRIHRRRLHRLVVG 60
           MA L+ISDRQIPLNSHDYYDITFD+D ILTL+TA  S+VDMWISEILRIHRRRLHRLVVG
Sbjct: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60

Query: 61  LDIEWRPNFNRRYRKPVATLQLCVGRRCLIFQLIHTSEIPESLIDFLEDDTFTFVGVGID 120
           LD+EWRPNF+R +R PVATLQLCVGRRCLIFQLIH SE P+SLIDFLEDDTFTFVGVGID
Sbjct: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120

Query: 121 QDVQQLYNDYDLKVANTVDLRELAANEMERDDLKNAGLKILGREVLGREIDKPRNVTLSQ 180
            DV +LYNDYDL VANTVDLRELAA+EM+ D+ + AGLK LGREVLGREIDKPRNV LS+
Sbjct: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180

Query: 181 WDRQCLSPGQVLYATVDAFLSFEIGR 207
           WDRQ L+P Q+LYATVDAFLSFEIGR
Sbjct: 181 WDRQWLNPAQILYATVDAFLSFEIGR 206

[Close]

BLAST of Bhi11G002328 vs. NCBI nr
Match: XP_022147765.1 (Werner Syndrome-like exonuclease, partial [Momordica charantia])

HSP 1 Score: 272.3 bits (695), Expect = 3.4e-69
Identity = 133/197 (67.51%), Postives = 157/197 (79.70%), Query Frame = 0


Query: 14  NSHDYYDITFDNDAILTLLTACLSVVDMWISEILRIHRRRLHRLVVGLDIEWRPNFNRRY 73
           NSH+ YDITFD+D I T +T+   +VD WIS ILR HRRRLHRL+VGLD+EWRPNFNR  
Sbjct: 5   NSHNCYDITFDSDQIKTTITSSPYMVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSR 64

Query: 74  RKPVATLQLCVGRRCLIFQLIHTSEIPESLIDFLEDDTFTFVGVGIDQDVQQLYNDYDLK 133
           R P ATLQLCVGRRCLIFQLIH  EIP SL+ FL D+TFTFVGVGIDQD ++L++DYDL 
Sbjct: 65  RNPAATLQLCVGRRCLIFQLIHAPEIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLT 124

Query: 134 VANTVDLRELAANEMERDDLKNAGLKILGREVLGREIDKPRNVTLSQWDRQCLSPGQVLY 193
           V N VDLR +A +   R DL+NAGLK L RE+LGREI KP+ VT+S+WD + L+  QV Y
Sbjct: 125 VNNAVDLRSMAVDRTGRRDLRNAGLKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAY 184

Query: 194 ATVDAFLSFEIGRYLLS 211
           A++DAFLSFEIGR L S
Sbjct: 185 ASIDAFLSFEIGRSLSS 201

[Close]

BLAST of Bhi11G002328 vs. NCBI nr
Match: XP_022011120.1 (Werner Syndrome-like exonuclease [Helianthus annuus] >KAF5766786.1 putative DNA helicase [Helianthus annuus])

HSP 1 Score: 267.7 bits (683), Expect = 8.5e-68
Identity = 128/207 (61.84%), Postives = 160/207 (77.29%), Query Frame = 0


Query: 4   LSISDRQIPLNSHDYYDITFDNDAILTLLTACLSVVDMWISEILRIHRRRLHRLVVGLDI 63
           +SIS+ Q+P N+HDY+D+TF ND ILTL+T   S VDMWIS+I RIHR RLH LVVGLDI
Sbjct: 3   ISISNHQVPYNTHDYFDVTFSNDTILTLVTHTPSYVDMWISDIERIHRHRLHSLVVGLDI 62

Query: 64  EWRPNFNRRYRKPVATLQLCVGRRCLIFQLIHTSEIPESLIDFLEDDTFTFVGVGIDQDV 123
           EWRP+FNR  + PVATLQLCVGRRCLIFQL+H   IP+SL++FL +  +TF GVGI+ DV
Sbjct: 63  EWRPSFNRNIQNPVATLQLCVGRRCLIFQLLHAPSIPQSLVNFLRNPNYTFTGVGIESDV 122

Query: 124 QQLYNDYDLKVANTVDLRELAANEMERDDLKNAGLKILGREVLGREIDKPRNVTLSQWDR 183
           ++L +DY+L V    DLR LAA      +LKN G+K L   VLG+E++KP+ VT+S+WD+
Sbjct: 123 EKLVDDYNLGVERMEDLRTLAAEAYGEWELKNGGIKSLAERVLGKEVNKPKWVTMSKWDQ 182

Query: 184 QCLSPGQVLYATVDAFLSFEIGRYLLS 211
           Q L P QV YA VDAFLSFEIG+ L+S
Sbjct: 183 QWLKPAQVQYACVDAFLSFEIGKVLIS 209

[Close]

BLAST of Bhi11G002328 vs. ExPASy TrEMBL
Match: A0A0A0LHC5 (3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G819830 PE=4 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 4.7e-96
Identity = 176/210 (83.81%), Postives = 192/210 (91.43%), Query Frame = 0


Query: 1   MAILSISDRQIPLNSHDYYDITFDNDAILTLLTACLSVVDMWISEILRIHRRRLHRLVVG 60
           MA L+ISDRQIPLNSHDYYDITFD+D ILTL+TA  S+VDMWISEILRIHRRRLHRLVVG
Sbjct: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60

Query: 61  LDIEWRPNFNRRYRKPVATLQLCVGRRCLIFQLIHTSEIPESLIDFLEDDTFTFVGVGID 120
           LD+EWRPNF+R +R PVATLQLCVGRRCLIFQLIH SE P+SLIDFLEDDTFTFVGVGID
Sbjct: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120

Query: 121 QDVQQLYNDYDLKVANTVDLRELAANEMERDDLKNAGLKILGREVLGREIDKPRNVTLSQ 180
            DV +LYNDYDL VANTVDLRELAA+EM+ D+ + AGLK LGREVLGREIDKPRNV LS+
Sbjct: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180

Query: 181 WDRQCLSPGQVLYATVDAFLSFEIGRYLLS 211
           WDRQ L+P Q+LYATVDAFLSFEIGRYLLS
Sbjct: 181 WDRQWLNPAQILYATVDAFLSFEIGRYLLS 210

[Close]

BLAST of Bhi11G002328 vs. ExPASy TrEMBL
Match: A0A6J1D121 (Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016622 PE=4 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 1.7e-69
Identity = 133/197 (67.51%), Postives = 157/197 (79.70%), Query Frame = 0


Query: 14  NSHDYYDITFDNDAILTLLTACLSVVDMWISEILRIHRRRLHRLVVGLDIEWRPNFNRRY 73
           NSH+ YDITFD+D I T +T+   +VD WIS ILR HRRRLHRL+VGLD+EWRPNFNR  
Sbjct: 5   NSHNCYDITFDSDQIKTTITSSPYMVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSR 64

Query: 74  RKPVATLQLCVGRRCLIFQLIHTSEIPESLIDFLEDDTFTFVGVGIDQDVQQLYNDYDLK 133
           R P ATLQLCVGRRCLIFQLIH  EIP SL+ FL D+TFTFVGVGIDQD ++L++DYDL 
Sbjct: 65  RNPAATLQLCVGRRCLIFQLIHAPEIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLT 124

Query: 134 VANTVDLRELAANEMERDDLKNAGLKILGREVLGREIDKPRNVTLSQWDRQCLSPGQVLY 193
           V N VDLR +A +   R DL+NAGLK L RE+LGREI KP+ VT+S+WD + L+  QV Y
Sbjct: 125 VNNAVDLRSMAVDRTGRRDLRNAGLKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAY 184

Query: 194 ATVDAFLSFEIGRYLLS 211
           A++DAFLSFEIGR L S
Sbjct: 185 ASIDAFLSFEIGRSLSS 201

[Close]

BLAST of Bhi11G002328 vs. ExPASy TrEMBL
Match: A0A251SDD2 (Putative ribonuclease H-like domain-containing protein OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr15g0495511 PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 2.0e-67
Identity = 128/207 (61.84%), Postives = 160/207 (77.29%), Query Frame = 0


Query: 4   LSISDRQIPLNSHDYYDITFDNDAILTLLTACLSVVDMWISEILRIHRRRLHRLVVGLDI 63
           +SISD Q+P N+HDY+D+TF ND ILTL+T   S VDMWISEI RIHR RLH LVVGLDI
Sbjct: 3   ISISDHQVPFNTHDYFDVTFSNDTILTLVTHTPSFVDMWISEIERIHRHRLHSLVVGLDI 62

Query: 64  EWRPNFNRRYRKPVATLQLCVGRRCLIFQLIHTSEIPESLIDFLEDDTFTFVGVGIDQDV 123
           EWRP+FNR  + PVATLQLCVGRRCLIFQL++   IP+SL++FL +  +TF GVGI+ DV
Sbjct: 63  EWRPSFNRNIQNPVATLQLCVGRRCLIFQLLYAPYIPQSLLNFLRNPNYTFTGVGIESDV 122

Query: 124 QQLYNDYDLKVANTVDLRELAANEMERDDLKNAGLKILGREVLGREIDKPRNVTLSQWDR 183
           ++L  DY+L V    D+R LAA      + KNAG+K L  +VLG+E++KP+ VT+S+WD+
Sbjct: 123 EKLVEDYNLGVERMEDVRTLAAEAYGVREFKNAGIKSLAEQVLGKEVNKPKWVTMSKWDQ 182

Query: 184 QCLSPGQVLYATVDAFLSFEIGRYLLS 211
             L+P QV YA VDAFLSFEIGR L+S
Sbjct: 183 PWLNPDQVQYACVDAFLSFEIGRVLIS 209

[Close]

BLAST of Bhi11G002328 vs. ExPASy TrEMBL
Match: A0A2I4GID7 (Werner Syndrome-like exonuclease OS=Juglans regia OX=51240 GN=LOC109008130 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 3.5e-67
Identity = 130/205 (63.41%), Postives = 160/205 (78.05%), Query Frame = 0


Query: 4   LSISDRQIPLNSHDYYDITFDNDAILTLLTACLSVVDMWISEILRIHRRRLHRLVVGLDI 63
           +SI D ++P ++H+ YD++FD+D I TLLT   S+VD WIS+I RIHRRRLHRLVVGLD+
Sbjct: 3   ISIYDYELPFDTHNLYDVSFDSDQIGTLLTCSPSLVDSWISDIYRIHRRRLHRLVVGLDL 62

Query: 64  EWRPNFNRRYRKPVATLQLCVGRRCLIFQLIHTSEIPESLIDFLEDDTFTFVGVGIDQDV 123
           EWRP+FNR  + PVATLQLCVGRRCLIFQLIH   IP+SL+DFL    FTFVGVGI  DV
Sbjct: 63  EWRPSFNRHVQNPVATLQLCVGRRCLIFQLIHAPYIPQSLVDFLGQTDFTFVGVGIKSDV 122

Query: 124 QQLYNDYDLKVANTVDLRELAANEMERDDLKNAGLKILGREVLGREIDKPRNVTLSQWDR 183
           ++L +DY L+VA  VDLR LA  E+    L+NAGLK L  EVLG+ I+KPRN+T+S+WD 
Sbjct: 123 EKLLDDYGLEVACVVDLRLLAVEELGTTQLRNAGLKQLAWEVLGKRIEKPRNITMSRWDN 182

Query: 184 QCLSPGQVLYATVDAFLSFEIGRYL 209
           + L+  QV YA +DAFLSFEIGR L
Sbjct: 183 EWLTCAQVQYACLDAFLSFEIGRQL 207

[Close]

BLAST of Bhi11G002328 vs. ExPASy TrEMBL
Match: A0A251SGI7 (Putative polynucleotidyl transferase, ribonuclease H-like superfamily protein OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr15g0495501 PE=4 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 7.7e-67
Identity = 126/202 (62.38%), Postives = 156/202 (77.23%), Query Frame = 0


Query: 4   LSISDRQIPLNSHDYYDITFDNDAILTLLTACLSVVDMWISEILRIHRRRLHRLVVGLDI 63
           +SIS+ Q+P N+HDY+D+TF ND ILTL+T   S VDMWIS+I RIHR RLH LVVGLDI
Sbjct: 3   ISISNHQVPYNTHDYFDVTFSNDTILTLVTHTPSYVDMWISDIERIHRHRLHSLVVGLDI 62

Query: 64  EWRPNFNRRYRKPVATLQLCVGRRCLIFQLIHTSEIPESLIDFLEDDTFTFVGVGIDQDV 123
           EWRP+FNR  + PVATLQLCVGRRCLIFQL+H   IP+SL++FL +  +TF GVGI+ DV
Sbjct: 63  EWRPSFNRNIQNPVATLQLCVGRRCLIFQLLHAPSIPQSLVNFLRNPNYTFTGVGIESDV 122

Query: 124 QQLYNDYDLKVANTVDLRELAANEMERDDLKNAGLKILGREVLGREIDKPRNVTLSQWDR 183
           ++L +DY+L V    DLR LAA      +LKN G+K L   VLG+E++KP+ VT+S+WD+
Sbjct: 123 EKLVDDYNLGVERMEDLRTLAAEAYGEWELKNGGIKSLAERVLGKEVNKPKWVTMSKWDQ 182

Query: 184 QCLSPGQVLYATVDAFLSFEIG 206
           Q L P QV YA VDAFLSFEIG
Sbjct: 183 QWLKPAQVQYACVDAFLSFEIG 204

The following BLAST results are available for this feature:

BLAST of Bhi11G002328 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G36110.1	1.5e-22	34.72	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G13870.2	6.3e-21	35.88	Werner syndrome-like exonuclease	[more]
AT3G12430.1	8.3e-21	30.14	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G13870.1	1.1e-20	36.14	Werner syndrome-like exonuclease	[more]
AT3G12460.1	3.1e-20	30.14	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

BLAST of Bhi11G002328 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84LH3	8.9e-20	35.88	Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=...	[more]
Q14191	3.8e-18	34.87	Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2	[more]
O09053	6.0e-16	33.57	Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn P...	[more]
Q9VGN7	7.8e-16	34.76	Exonuclease 3'-5' domain-containing protein 2 OS=Drosophila melanogaster OX=7227...	[more]
O93530	1.5e-14	32.47	Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn ...	[more]

BLAST of Bhi11G002328 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038906336.1	1.6e-114	100.00	Werner Syndrome-like exonuclease [Benincasa hispida]	[more]
XP_004136771.1	9.6e-96	83.81	Werner Syndrome-like exonuclease [Cucumis sativus]	[more]
KAE8651170.1	1.2e-93	83.50	hypothetical protein Csa_000755 [Cucumis sativus]	[more]
XP_022147765.1	3.4e-69	67.51	Werner Syndrome-like exonuclease, partial [Momordica charantia]	[more]
XP_022011120.1	8.5e-68	61.84	Werner Syndrome-like exonuclease [Helianthus annuus] >KAF5766786.1 putative DNA ...	[more]

BLAST of Bhi11G002328 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHC5	4.7e-96	83.81	3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
A0A6J1D121	1.7e-69	67.51	Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016622 ...	[more]
A0A251SDD2	2.0e-67	61.84	Putative ribonuclease H-like domain-containing protein OS=Helianthus annuus OX=4...	[more]
A0A2I4GID7	3.5e-67	63.41	Werner Syndrome-like exonuclease OS=Juglans regia OX=51240 GN=LOC109008130 PE=4 ...	[more]
A0A251SGI7	7.7e-67	62.38	Putative polynucleotidyl transferase, ribonuclease H-like superfamily protein OS...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002562	3'-5' exonuclease domain	SMART	SM00474	35exoneu6	coord: 36..210 e-value: 9.1E-5 score: 31.8
IPR002562	3'-5' exonuclease domain	PFAM	PF01612	DNA_pol_A_exo1	coord: 53..208 e-value: 1.6E-15 score: 57.3
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 19..209 e-value: 9.0E-59 score: 200.3
None	No IPR available	PANTHER	PTHR13620	3-5 EXONUCLEASE	coord: 10..208
None	No IPR available	PANTHER	PTHR13620:SF65	OS01G0660800 PROTEIN	coord: 10..208
None	No IPR available	CDD	cd06141	WRN_exo	coord: 33..209 e-value: 1.47587E-60 score: 185.089
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 41..209

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi11M002328	Bhi11M002328	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0006139	nucleobase-containing compound metabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding