Bhi11G001509 (gene) Wax gourd (B227) v1

Overview
NameBhi11G001509
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptiontranscription factor-related
Locationchr11: 50710526 .. 50711191 (+)
RNA-Seq ExpressionBhi11G001509
SyntenyBhi11G001509
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAGGGGACCATTACAATGCTGACTCATTCAAAGCATTTTTCGATGCTTGGCTCCTCCGCCAACGTAACTTCCTCGACGACCTTCTCTCCGCCGAAAGCGGCAGTCCTCACGACGGCGATCTCCAAGATTTAATCTCTCGAGTCCTTAGCCACTACGAAGACTATTATGAGAAAAAATCAAGAATTGCCCAAACAGATATTTTTCTCGTTTTCTCCCCTCCCTGGTTCACAACTTACGAAAGGACCCTCCTCTGGATCGGTGGATTCCGGCCGGGACTGACTTTCCGGCTGGTCAATCAGTCCATTAACGACATGTCGGACGAACAGGTTCTCAGAATTAGGCGCCTGAGGGACGACGTGAAGATTGAAGAGCGGCTACTCAACAATGACTTGGCCAAAATTCAAGAAAAAGTGGCGGCACCGCCGTTGCTGGAATTTTTCCGACGCGGTGGTCACGACGGCGTTATTGGCGGCGAGGAGGCAATGGAGAATCTAAAAGCGGCGTTTCAGTCGGTGCTGGCGAGTGCGGATTTTTTTAGAAGAGATACGGCGTTGAAAGTGACGGAGATTCTTACTCCGGCGCAGGCCGTTAGATTTTGGGCCGCCGTGGCTCAGCTTCACCTTAGAATCAGAGCGTTGGGTTTGCAAGAAGATGCCGAGTGA

mRNA sequence

ATGCCAGGGGACCATTACAATGCTGACTCATTCAAAGCATTTTTCGATGCTTGGCTCCTCCGCCAACGTAACTTCCTCGACGACCTTCTCTCCGCCGAAAGCGGCAGTCCTCACGACGGCGATCTCCAAGATTTAATCTCTCGAGTCCTTAGCCACTACGAAGACTATTATGAGAAAAAATCAAGAATTGCCCAAACAGATATTTTTCTCGTTTTCTCCCCTCCCTGGTTCACAACTTACGAAAGGACCCTCCTCTGGATCGGTGGATTCCGGCCGGGACTGACTTTCCGGCTGGTCAATCAGTCCATTAACGACATGTCGGACGAACAGGTTCTCAGAATTAGGCGCCTGAGGGACGACGTGAAGATTGAAGAGCGGCTACTCAACAATGACTTGGCCAAAATTCAAGAAAAAGTGGCGGCACCGCCGTTGCTGGAATTTTTCCGACGCGGTGGTCACGACGGCGTTATTGGCGGCGAGGAGGCAATGGAGAATCTAAAAGCGGCGTTTCAGTCGGTGCTGGCGAGTGCGGATTTTTTTAGAAGAGATACGGCGTTGAAAGTGACGGAGATTCTTACTCCGGCGCAGGCCGTTAGATTTTGGGCCGCCGTGGCTCAGCTTCACCTTAGAATCAGAGCGTTGGGTTTGCAAGAAGATGCCGAGTGA

Coding sequence (CDS)

ATGCCAGGGGACCATTACAATGCTGACTCATTCAAAGCATTTTTCGATGCTTGGCTCCTCCGCCAACGTAACTTCCTCGACGACCTTCTCTCCGCCGAAAGCGGCAGTCCTCACGACGGCGATCTCCAAGATTTAATCTCTCGAGTCCTTAGCCACTACGAAGACTATTATGAGAAAAAATCAAGAATTGCCCAAACAGATATTTTTCTCGTTTTCTCCCCTCCCTGGTTCACAACTTACGAAAGGACCCTCCTCTGGATCGGTGGATTCCGGCCGGGACTGACTTTCCGGCTGGTCAATCAGTCCATTAACGACATGTCGGACGAACAGGTTCTCAGAATTAGGCGCCTGAGGGACGACGTGAAGATTGAAGAGCGGCTACTCAACAATGACTTGGCCAAAATTCAAGAAAAAGTGGCGGCACCGCCGTTGCTGGAATTTTTCCGACGCGGTGGTCACGACGGCGTTATTGGCGGCGAGGAGGCAATGGAGAATCTAAAAGCGGCGTTTCAGTCGGTGCTGGCGAGTGCGGATTTTTTTAGAAGAGATACGGCGTTGAAAGTGACGGAGATTCTTACTCCGGCGCAGGCCGTTAGATTTTGGGCCGCCGTGGCTCAGCTTCACCTTAGAATCAGAGCGTTGGGTTTGCAAGAAGATGCCGAGTGA

Protein sequence

MPGDHYNADSFKAFFDAWLLRQRNFLDDLLSAESGSPHDGDLQDLISRVLSHYEDYYEKKSRIAQTDIFLVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRRLRDDVKIEERLLNNDLAKIQEKVAAPPLLEFFRRGGHDGVIGGEEAMENLKAAFQSVLASADFFRRDTALKVTEILTPAQAVRFWAAVAQLHLRIRALGLQEDAE
Homology
BLAST of Bhi11G001509 vs. TAIR 10
Match: AT1G09950.1 (RESPONSE TO ABA AND SALT 1 )

HSP 1 Score: 151.0 bits (380), Expect = 1.1e-36
Identity = 83/218 (38.07%), Postives = 126/218 (57.80%), Query Frame = 0

Query: 7   NADSFKAFFDAWLLRQRNFLDDLLSAESGSPHD----GDLQDLISRVLSHYEDYYEKK-- 66
           ++ SF  F D WL+R R F++ L+ A S    +     + Q L+++ LSH   YY++K  
Sbjct: 6   SSQSFTIFVDGWLIRHRYFVEQLMCASSLDETNRISLEEQQSLVAQFLSHCLQYYQEKFA 65

Query: 67  -SRIAQTDIFLVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRRLRD 126
              +A  ++F  F PPWF +Y + +LW+G F+P L F+L   S+ D++  Q  RI  L+ 
Sbjct: 66  SVSLAGDNVFTFFCPPWFNSYAKLILWVGDFKPSLVFKLTEVSVADLTRHQKDRISSLKS 125

Query: 127 DVKIEERLLNNDLAKIQEKVAAPPLLEFFRRGGHDGVIGG-----EEAMENLKAAFQSVL 186
           + + +ER +  D A +Q+ VA PP++   RR G  G++ G     EEAME LKA   + +
Sbjct: 126 ETRRKEREVMRDFALVQQSVADPPVMLAARRVGAVGMVDGEETDLEEAMEVLKAGMAAAM 185

Query: 187 ASADFFRRDTALKVTEILTPAQAVRFWAAVAQLHLRIR 213
            +AD  R  T  KV EILTP QA++    + QLHLR+R
Sbjct: 186 NNADQLRCSTVGKVVEILTPPQAIKVLRTIGQLHLRLR 223

BLAST of Bhi11G001509 vs. TAIR 10
Match: AT1G58330.1 (transcription factor-related )

HSP 1 Score: 146.7 bits (369), Expect = 2.1e-35
Identity = 83/220 (37.73%), Postives = 129/220 (58.64%), Query Frame = 0

Query: 7   NADSFKAFFDAWLLRQRNFLDDL--LSAESGSPHDGDLQDLISRVLSHYEDYYEKKS--- 66
           ++++F +FF+ WL R R F+  L  L+ E+      + + L+S  LSHY  YYE+KS   
Sbjct: 6   SSETFASFFNDWLCRHRQFVQQLAHLADETTIVTPIEEESLVSNFLSHYLQYYEEKSVAM 65

Query: 67  RIAQTDIFLVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRRLRDDV 126
            +A  DI+  FSPPW ++YE+ +LWIGGF+PG+ F+L+  S+ND++  Q+ ++  +R + 
Sbjct: 66  SVAGDDIYDFFSPPWLSSYEKLILWIGGFKPGMVFKLITTSVNDLTSHQIDQLESIRLET 125

Query: 127 KIEERLLNNDLAKIQEKVAAPPLLEFFRRGGHDGVIGG-----EEAMENLKAAFQSVLAS 186
           K  ER L    A +Q+ V  P L+  FRR G   +  G     E+AME LK      + +
Sbjct: 126 KRRERDLMRRFALLQQSVGDPLLMVPFRRIGVLRLGEGEQPEMEDAMEVLKVEMIKAMKN 185

Query: 187 ADFFRRDTALKVTEILTPAQAVRFWAAVAQLHLRIRALGL 217
           AD  R  T  KV E+L P Q+++   A  + +LR+R LG+
Sbjct: 186 ADQLRCVTVGKVVEVLNPRQSIKLLRAAGEFYLRLRDLGV 225

BLAST of Bhi11G001509 vs. TAIR 10
Match: AT1G77920.1 (bZIP transcription factor family protein )

HSP 1 Score: 99.0 bits (245), Expect = 5.1e-21
Identity = 61/205 (29.76%), Postives = 106/205 (51.71%), Query Frame = 0

Query: 10  SFKAFFDAWLLRQRNFLDDLLSAESGSPHDGDLQDLISRVLSHYEDYYEKKSRIAQTDIF 69
           SF+  +  WL  Q   + +L +A      D +L+ L+   L+HY + ++ KS  A+ D+F
Sbjct: 154 SFEMEYSHWLQEQSRRVSELRTALQSHISDIELKMLVESCLNHYANLFQMKSDAAKADVF 213

Query: 70  LVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRRLRDDVKIEERLLN 129
            + S  W T+ ER   WIGGFRP     +V   +  ++D+Q+L +R L+   +  E  L+
Sbjct: 214 YLISGMWRTSTERFFQWIGGFRPSELLNVVMPYLQPLTDQQILEVRNLQQSSQQAEDALS 273

Query: 130 NDLAKIQEKVAAPPLLEFFRRGGHDGVIGGEEAMENLKAAFQSVLASADFFRRDTALKVT 189
             + K+Q+ +A   +++      H        A+ENL+ A +  +  AD  R+ T  ++ 
Sbjct: 274 QGIDKLQQSLAESIVIDAVIESTHYPT-HMAAAIENLQ-ALEGFVNQADHLRQQTLQQMA 333

Query: 190 EILTPAQAVRFWAAVAQLHLRIRAL 215
           +ILT  Q+ R   A+ +   R+RAL
Sbjct: 334 KILTTRQSARGLLALGEYLHRLRAL 356

BLAST of Bhi11G001509 vs. TAIR 10
Match: AT1G22070.1 (TGA1A-related gene 3 )

HSP 1 Score: 97.8 bits (242), Expect = 1.1e-20
Identity = 60/205 (29.27%), Postives = 105/205 (51.22%), Query Frame = 0

Query: 10  SFKAFFDAWLLRQRNFLDDLLSAESGSPHDGDLQDLISRVLSHYEDYYEKKSRIAQTDIF 69
           +F+  +  WL  Q   + ++ +A      D +L+ L+   L+HY + +  K+  A+ D+F
Sbjct: 169 AFEMEYTHWLEEQNRRVSEIRTALQAHIGDIELKMLVDSCLNHYANLFRMKADAAKADVF 228

Query: 70  LVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRRLRDDVKIEERLLN 129
            + S  W T+ ER   WIGGFRP     +V   +  ++D+Q+L +R L+   +  E  L+
Sbjct: 229 FLMSGMWRTSTERFFQWIGGFRPSELLNVVMPYVEPLTDQQLLEVRNLQQSSQQAEEALS 288

Query: 130 NDLAKIQEKVAAPPLLEFFRRGGHDGVIGGEEAMENLKAAFQSVLASADFFRRDTALKVT 189
             L K+Q+ +     ++       +       AMENL+ A +S +  AD  R+ T  +++
Sbjct: 289 QGLDKLQQGLVESIAIQIKVVESVNHGAPMASAMENLQ-ALESFVNQADHLRQQTLQQMS 348

Query: 190 EILTPAQAVRFWAAVAQLHLRIRAL 215
           +ILT  QA R   A+ +   R+RAL
Sbjct: 349 KILTTRQAARGLLALGEYFHRLRAL 372

BLAST of Bhi11G001509 vs. TAIR 10
Match: AT5G10030.1 (TGACG motif-binding factor 4 )

HSP 1 Score: 94.0 bits (232), Expect = 1.6e-19
Identity = 61/205 (29.76%), Postives = 100/205 (48.78%), Query Frame = 0

Query: 10  SFKAFFDAWLLRQRNFLDDLLSAESGSPHDGDLQDLISRVLSHYEDYYEKKSRIAQTDIF 69
           +F+  +  W+  Q   + +L +   G   D +L+ L+   + HY   +  KS  A+ D+F
Sbjct: 151 AFEMEYGHWVEEQNRQICELRTVLHGQVSDIELRSLVENAMKHYFQLFRMKSAAAKIDVF 210

Query: 70  LVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRRLRDDVKIEERLLN 129
            V S  W T+ ER  LWIGGFRP    +++    + ++D+Q+L +  LR   +  E  L+
Sbjct: 211 YVMSGMWKTSAERFFLWIGGFRPSELLKVLLPHFDPLTDQQLLDVCNLRQSCQQAEDALS 270

Query: 130 NDLAKIQEKVAAPPLLEFFRRGGHDGVIGGEEAMENLKAAFQSVLASADFFRRDTALKVT 189
             + K+Q  +A          G +  +     AME L+ A  S +  AD  R +T  ++ 
Sbjct: 271 QGMEKLQHTLAESVAAGKLGEGSY--IPQMTCAMERLE-ALVSFVNQADHLRHETLQQMH 330

Query: 190 EILTPAQAVRFWAAVAQLHLRIRAL 215
            ILT  QA R   A+ +   R+RAL
Sbjct: 331 RILTTRQAARGLLALGEYFQRLRAL 352

BLAST of Bhi11G001509 vs. ExPASy Swiss-Prot
Match: O04515 (Protein RESPONSE TO ABA AND SALT 1 OS=Arabidopsis thaliana OX=3702 GN=RAS1 PE=2 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.6e-35
Identity = 83/218 (38.07%), Postives = 126/218 (57.80%), Query Frame = 0

Query: 7   NADSFKAFFDAWLLRQRNFLDDLLSAESGSPHD----GDLQDLISRVLSHYEDYYEKK-- 66
           ++ SF  F D WL+R R F++ L+ A S    +     + Q L+++ LSH   YY++K  
Sbjct: 6   SSQSFTIFVDGWLIRHRYFVEQLMCASSLDETNRISLEEQQSLVAQFLSHCLQYYQEKFA 65

Query: 67  -SRIAQTDIFLVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRRLRD 126
              +A  ++F  F PPWF +Y + +LW+G F+P L F+L   S+ D++  Q  RI  L+ 
Sbjct: 66  SVSLAGDNVFTFFCPPWFNSYAKLILWVGDFKPSLVFKLTEVSVADLTRHQKDRISSLKS 125

Query: 127 DVKIEERLLNNDLAKIQEKVAAPPLLEFFRRGGHDGVIGG-----EEAMENLKAAFQSVL 186
           + + +ER +  D A +Q+ VA PP++   RR G  G++ G     EEAME LKA   + +
Sbjct: 126 ETRRKEREVMRDFALVQQSVADPPVMLAARRVGAVGMVDGEETDLEEAMEVLKAGMAAAM 185

Query: 187 ASADFFRRDTALKVTEILTPAQAVRFWAAVAQLHLRIR 213
            +AD  R  T  KV EILTP QA++    + QLHLR+R
Sbjct: 186 NNADQLRCSTVGKVVEILTPPQAIKVLRTIGQLHLRLR 223

BLAST of Bhi11G001509 vs. ExPASy Swiss-Prot
Match: Q9SLV1 (Protein ZW2 OS=Arabidopsis thaliana OX=3702 GN=ZW2 PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 3.0e-34
Identity = 83/220 (37.73%), Postives = 129/220 (58.64%), Query Frame = 0

Query: 7   NADSFKAFFDAWLLRQRNFLDDL--LSAESGSPHDGDLQDLISRVLSHYEDYYEKKS--- 66
           ++++F +FF+ WL R R F+  L  L+ E+      + + L+S  LSHY  YYE+KS   
Sbjct: 6   SSETFASFFNDWLCRHRQFVQQLAHLADETTIVTPIEEESLVSNFLSHYLQYYEEKSVAM 65

Query: 67  RIAQTDIFLVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRRLRDDV 126
            +A  DI+  FSPPW ++YE+ +LWIGGF+PG+ F+L+  S+ND++  Q+ ++  +R + 
Sbjct: 66  SVAGDDIYDFFSPPWLSSYEKLILWIGGFKPGMVFKLITTSVNDLTSHQIDQLESIRLET 125

Query: 127 KIEERLLNNDLAKIQEKVAAPPLLEFFRRGGHDGVIGG-----EEAMENLKAAFQSVLAS 186
           K  ER L    A +Q+ V  P L+  FRR G   +  G     E+AME LK      + +
Sbjct: 126 KRRERDLMRRFALLQQSVGDPLLMVPFRRIGVLRLGEGEQPEMEDAMEVLKVEMIKAMKN 185

Query: 187 ADFFRRDTALKVTEILTPAQAVRFWAAVAQLHLRIRALGL 217
           AD  R  T  KV E+L P Q+++   A  + +LR+R LG+
Sbjct: 186 ADQLRCVTVGKVVEVLNPRQSIKLLRAAGEFYLRLRDLGV 225

BLAST of Bhi11G001509 vs. ExPASy Swiss-Prot
Match: Q93ZE2 (Transcription factor TGA7 OS=Arabidopsis thaliana OX=3702 GN=TGA7 PE=1 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 7.2e-20
Identity = 61/205 (29.76%), Postives = 106/205 (51.71%), Query Frame = 0

Query: 10  SFKAFFDAWLLRQRNFLDDLLSAESGSPHDGDLQDLISRVLSHYEDYYEKKSRIAQTDIF 69
           SF+  +  WL  Q   + +L +A      D +L+ L+   L+HY + ++ KS  A+ D+F
Sbjct: 154 SFEMEYSHWLQEQSRRVSELRTALQSHISDIELKMLVESCLNHYANLFQMKSDAAKADVF 213

Query: 70  LVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRRLRDDVKIEERLLN 129
            + S  W T+ ER   WIGGFRP     +V   +  ++D+Q+L +R L+   +  E  L+
Sbjct: 214 YLISGMWRTSTERFFQWIGGFRPSELLNVVMPYLQPLTDQQILEVRNLQQSSQQAEDALS 273

Query: 130 NDLAKIQEKVAAPPLLEFFRRGGHDGVIGGEEAMENLKAAFQSVLASADFFRRDTALKVT 189
             + K+Q+ +A   +++      H        A+ENL+ A +  +  AD  R+ T  ++ 
Sbjct: 274 QGIDKLQQSLAESIVIDAVIESTHYPT-HMAAAIENLQ-ALEGFVNQADHLRQQTLQQMA 333

Query: 190 EILTPAQAVRFWAAVAQLHLRIRAL 215
           +ILT  Q+ R   A+ +   R+RAL
Sbjct: 334 KILTTRQSARGLLALGEYLHRLRAL 356

BLAST of Bhi11G001509 vs. ExPASy Swiss-Prot
Match: Q39234 (Transcription factor TGA3 OS=Arabidopsis thaliana OX=3702 GN=TGA3 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.6e-19
Identity = 60/205 (29.27%), Postives = 105/205 (51.22%), Query Frame = 0

Query: 10  SFKAFFDAWLLRQRNFLDDLLSAESGSPHDGDLQDLISRVLSHYEDYYEKKSRIAQTDIF 69
           +F+  +  WL  Q   + ++ +A      D +L+ L+   L+HY + +  K+  A+ D+F
Sbjct: 169 AFEMEYTHWLEEQNRRVSEIRTALQAHIGDIELKMLVDSCLNHYANLFRMKADAAKADVF 228

Query: 70  LVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRRLRDDVKIEERLLN 129
            + S  W T+ ER   WIGGFRP     +V   +  ++D+Q+L +R L+   +  E  L+
Sbjct: 229 FLMSGMWRTSTERFFQWIGGFRPSELLNVVMPYVEPLTDQQLLEVRNLQQSSQQAEEALS 288

Query: 130 NDLAKIQEKVAAPPLLEFFRRGGHDGVIGGEEAMENLKAAFQSVLASADFFRRDTALKVT 189
             L K+Q+ +     ++       +       AMENL+ A +S +  AD  R+ T  +++
Sbjct: 289 QGLDKLQQGLVESIAIQIKVVESVNHGAPMASAMENLQ-ALESFVNQADHLRQQTLQQMS 348

Query: 190 EILTPAQAVRFWAAVAQLHLRIRAL 215
           +ILT  QA R   A+ +   R+RAL
Sbjct: 349 KILTTRQAARGLLALGEYFHRLRAL 372

BLAST of Bhi11G001509 vs. ExPASy Swiss-Prot
Match: Q0JNL3 (Transcription factor TGA2.3 OS=Oryza sativa subsp. japonica OX=39947 GN=TGA2.3 PE=1 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 4.7e-19
Identity = 57/208 (27.40%), Postives = 103/208 (49.52%), Query Frame = 0

Query: 10  SFKAFFDAWLLRQRNFLDDLLSAESGSPHDGDLQDLISRVLSHYEDYYEKKSRIAQTDIF 69
           +F   +  WL      +++L SA +    D +L+ ++ +++SHYE+ +++K   A+ D+F
Sbjct: 112 AFDMEYARWLEEHNRQINELRSAVNAHAGDNELRGVVDKIMSHYEEIFKQKGNAAKADVF 171

Query: 70  LVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRRLRDDVKIEERLLN 129
            V S  W T  ER  LW+GGFRP    +L++  +  ++++Q+  I  L+   +  E  L+
Sbjct: 172 HVLSGMWKTPAERCFLWLGGFRPSELLKLLSTQLEPLTEQQLSGIANLQQSSQQAEDALS 231

Query: 130 NDLAKIQEKVAAPPLLEFFRRGGHDGVIG--GEEAMENLK-AAFQSVLASADFFRRDTAL 189
             +  +Q+ +A          G    V    G+ AM   K    ++ L  AD  R+ T  
Sbjct: 232 QGMEALQQSLAETLAGSLGSSGSTGNVANYMGQMAMAMGKLGTLENFLRQADNLRQQTLQ 291

Query: 190 KVTEILTPAQAVRFWAAVAQLHLRIRAL 215
           ++  ILT  Q+ R    ++    R+RAL
Sbjct: 292 QMQRILTTRQSARALLVISDYSSRLRAL 319

BLAST of Bhi11G001509 vs. NCBI nr
Match: XP_038905753.1 (protein RESPONSE TO ABA AND SALT 1-like [Benincasa hispida])

HSP 1 Score: 444.5 bits (1142), Expect = 5.3e-121
Identity = 221/221 (100.00%), Postives = 221/221 (100.00%), Query Frame = 0

Query: 1   MPGDHYNADSFKAFFDAWLLRQRNFLDDLLSAESGSPHDGDLQDLISRVLSHYEDYYEKK 60
           MPGDHYNADSFKAFFDAWLLRQRNFLDDLLSAESGSPHDGDLQDLISRVLSHYEDYYEKK
Sbjct: 1   MPGDHYNADSFKAFFDAWLLRQRNFLDDLLSAESGSPHDGDLQDLISRVLSHYEDYYEKK 60

Query: 61  SRIAQTDIFLVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRRLRDD 120
           SRIAQTDIFLVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRRLRDD
Sbjct: 61  SRIAQTDIFLVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRRLRDD 120

Query: 121 VKIEERLLNNDLAKIQEKVAAPPLLEFFRRGGHDGVIGGEEAMENLKAAFQSVLASADFF 180
           VKIEERLLNNDLAKIQEKVAAPPLLEFFRRGGHDGVIGGEEAMENLKAAFQSVLASADFF
Sbjct: 121 VKIEERLLNNDLAKIQEKVAAPPLLEFFRRGGHDGVIGGEEAMENLKAAFQSVLASADFF 180

Query: 181 RRDTALKVTEILTPAQAVRFWAAVAQLHLRIRALGLQEDAE 222
           RRDTALKVTEILTPAQAVRFWAAVAQLHLRIRALGLQEDAE
Sbjct: 181 RRDTALKVTEILTPAQAVRFWAAVAQLHLRIRALGLQEDAE 221

BLAST of Bhi11G001509 vs. NCBI nr
Match: XP_008442399.1 (PREDICTED: transcription factor HBP-1b(c38) [Cucumis melo] >KAA0044183.1 transcription factor HBP-1b(c38) [Cucumis melo var. makuwa] >TYK24949.1 transcription factor HBP-1b(c38) [Cucumis melo var. makuwa])

HSP 1 Score: 389.8 bits (1000), Expect = 1.6e-104
Identity = 192/220 (87.27%), Postives = 204/220 (92.73%), Query Frame = 0

Query: 1   MPGDHYNADSFKAFFDAWLLRQRNFLDDLLSAESGSPHDGDLQDLISRVLSHYEDYYEKK 60
           M GDHYNADSFKAFF+AWLLRQRN+LDDLLSA  GSPH+ DLQ  ISR+LSHYEDYYEKK
Sbjct: 1   MAGDHYNADSFKAFFEAWLLRQRNYLDDLLSAAHGSPHNRDLQVSISRILSHYEDYYEKK 60

Query: 61  SRIAQTDIFLVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRRLRDD 120
           SRIAQTDIFLVF+PPWFTTYERTLLWIGGFRPGL  RLVNQSI+D+SDEQV+RIRRL+DD
Sbjct: 61  SRIAQTDIFLVFTPPWFTTYERTLLWIGGFRPGLIIRLVNQSIDDLSDEQVVRIRRLKDD 120

Query: 121 VKIEERLLNNDLAKIQEKVAAPPLLEFFRRGGHDGVIGGEEAMENLKAAFQSVLASADFF 180
            KIEERLLNNDLAKIQEKVAAPPLLEFFR GGHDG +GGEEAME LKAAFQSVLASAD  
Sbjct: 121 TKIEERLLNNDLAKIQEKVAAPPLLEFFRHGGHDGAVGGEEAMETLKAAFQSVLASADLL 180

Query: 181 RRDTALKVTEILTPAQAVRFWAAVAQLHLRIRALGLQEDA 221
           RRDTALKVT+ILTPAQ VRF AAVAQLHLR+RALGLQEDA
Sbjct: 181 RRDTALKVTQILTPAQTVRFLAAVAQLHLRVRALGLQEDA 220

BLAST of Bhi11G001509 vs. NCBI nr
Match: XP_031737579.1 (protein RESPONSE TO ABA AND SALT 1 [Cucumis sativus] >KGN58743.1 hypothetical protein Csa_001384 [Cucumis sativus])

HSP 1 Score: 382.5 bits (981), Expect = 2.5e-102
Identity = 188/221 (85.07%), Postives = 204/221 (92.31%), Query Frame = 0

Query: 1   MPGDHYNADSFKAFFDAWLLRQRNFLDDLLSAESGSPHDGDLQDLISRVLSHYEDYYEKK 60
           M GDHYNADSFKAFF+AWLLRQRN+LDDLLS   G+P + DLQ  ISR+LSHYEDYYEKK
Sbjct: 1   MAGDHYNADSFKAFFEAWLLRQRNYLDDLLSTAHGTPQNRDLQVSISRILSHYEDYYEKK 60

Query: 61  SRIAQTDIFLVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRRLRDD 120
           SRIAQTDIFLVF+PPWFTTYE+TLLWIGGFRPGL  RLVNQSI+D+SDEQV+RIRRL+DD
Sbjct: 61  SRIAQTDIFLVFTPPWFTTYEKTLLWIGGFRPGLIVRLVNQSIDDLSDEQVVRIRRLKDD 120

Query: 121 VKIEERLLNNDLAKIQEKVAAPPLLEFFRRGGHDGVIGGEEAMENLKAAFQSVLASADFF 180
            K+EERLLNNDLAKIQEKVAAPPLLEFFR GGHDGVIGGE AME+LKAAFQSVLASAD  
Sbjct: 121 TKVEERLLNNDLAKIQEKVAAPPLLEFFRHGGHDGVIGGEAAMESLKAAFQSVLASADLL 180

Query: 181 RRDTALKVTEILTPAQAVRFWAAVAQLHLRIRALGLQEDAE 222
           RRDTALKVT+ILTPAQ VRF AAVAQLHLR+RALGLQEDA+
Sbjct: 181 RRDTALKVTQILTPAQTVRFLAAVAQLHLRVRALGLQEDAK 221

BLAST of Bhi11G001509 vs. NCBI nr
Match: XP_022944640.1 (protein DOG1-like 4 [Cucurbita moschata])

HSP 1 Score: 350.5 bits (898), Expect = 1.0e-92
Identity = 179/225 (79.56%), Postives = 195/225 (86.67%), Query Frame = 0

Query: 1   MPGDHYNADSFKAFFDAWLLRQRNFLDDLLSAESGSPHD----GDLQDLISRVLSHYEDY 60
           MPGD YN DSF AFF+ WLLRQ+ +LD+LL+A    P D    GDL DLISR LSHYE+Y
Sbjct: 1   MPGDDYNVDSFTAFFEGWLLRQQQYLDELLTATRRHPDDAADNGDLDDLISRTLSHYEEY 60

Query: 61  YEKKSRIAQTDIFLVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRR 120
           YEKKSRIAQ DIFLVFSP WFTTYE++LLWIGGFRPGL FRLVN+SIND+ D+QVLRI R
Sbjct: 61  YEKKSRIAQRDIFLVFSPTWFTTYEQSLLWIGGFRPGLIFRLVNESINDLWDDQVLRIGR 120

Query: 121 LRDDVKIEERLLNNDLAKIQEKVAAPPLLEFFRRGGHDGVIGGEEAMENLKAAFQSVLAS 180
           LR+DVK+EER+LNNDLAKIQEKVAAPPLLEFFRR GHDGV GG E ME LKAAFQSVLAS
Sbjct: 121 LREDVKVEERMLNNDLAKIQEKVAAPPLLEFFRRQGHDGVTGGTE-MEALKAAFQSVLAS 180

Query: 181 ADFFRRDTALKVTEILTPAQAVRFWAAVAQLHLRIRALGLQEDAE 222
           ADFFRR+TALKV EILTPAQ VRF AAVAQLHLRIRA GLQ+DAE
Sbjct: 181 ADFFRRETALKVAEILTPAQTVRFLAAVAQLHLRIRAYGLQKDAE 224

BLAST of Bhi11G001509 vs. NCBI nr
Match: KAG6596339.1 (Protein RESPONSE TO ABA AND SALT 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 348.2 bits (892), Expect = 5.2e-92
Identity = 178/225 (79.11%), Postives = 194/225 (86.22%), Query Frame = 0

Query: 1   MPGDHYNADSFKAFFDAWLLRQRNFLDDLLSAESGSPHD----GDLQDLISRVLSHYEDY 60
           MPGD YN DSF AFF+ WLLRQ+ +LD+LL+A    P D    GDL DLISR LSHYE+Y
Sbjct: 1   MPGDDYNVDSFTAFFEGWLLRQQQYLDELLTATRRHPDDAADNGDLDDLISRTLSHYEEY 60

Query: 61  YEKKSRIAQTDIFLVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRR 120
           YEKKSRIAQ DIFLVFSP WFTTYE++LLWIGGFRPGL FRLVN+SIND+ D+QVLRI R
Sbjct: 61  YEKKSRIAQRDIFLVFSPTWFTTYEQSLLWIGGFRPGLIFRLVNESINDLWDDQVLRIGR 120

Query: 121 LRDDVKIEERLLNNDLAKIQEKVAAPPLLEFFRRGGHDGVIGGEEAMENLKAAFQSVLAS 180
           LR+DVK+EER+LNNDLAKIQEKVAAPPLLEFFRR GHDGV GG E ME LKAAFQSVLAS
Sbjct: 121 LREDVKVEERMLNNDLAKIQEKVAAPPLLEFFRRQGHDGVTGGTE-MEALKAAFQSVLAS 180

Query: 181 ADFFRRDTALKVTEILTPAQAVRFWAAVAQLHLRIRALGLQEDAE 222
           ADF RR+TALKV EILTPAQ VRF AAVAQLHLRIRA GLQ+DAE
Sbjct: 181 ADFLRRETALKVAEILTPAQTVRFLAAVAQLHLRIRACGLQKDAE 224

BLAST of Bhi11G001509 vs. ExPASy TrEMBL
Match: A0A5A7TKZ8 (Transcription factor HBP-1b(C38) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00650 PE=4 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 7.5e-105
Identity = 192/220 (87.27%), Postives = 204/220 (92.73%), Query Frame = 0

Query: 1   MPGDHYNADSFKAFFDAWLLRQRNFLDDLLSAESGSPHDGDLQDLISRVLSHYEDYYEKK 60
           M GDHYNADSFKAFF+AWLLRQRN+LDDLLSA  GSPH+ DLQ  ISR+LSHYEDYYEKK
Sbjct: 1   MAGDHYNADSFKAFFEAWLLRQRNYLDDLLSAAHGSPHNRDLQVSISRILSHYEDYYEKK 60

Query: 61  SRIAQTDIFLVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRRLRDD 120
           SRIAQTDIFLVF+PPWFTTYERTLLWIGGFRPGL  RLVNQSI+D+SDEQV+RIRRL+DD
Sbjct: 61  SRIAQTDIFLVFTPPWFTTYERTLLWIGGFRPGLIIRLVNQSIDDLSDEQVVRIRRLKDD 120

Query: 121 VKIEERLLNNDLAKIQEKVAAPPLLEFFRRGGHDGVIGGEEAMENLKAAFQSVLASADFF 180
            KIEERLLNNDLAKIQEKVAAPPLLEFFR GGHDG +GGEEAME LKAAFQSVLASAD  
Sbjct: 121 TKIEERLLNNDLAKIQEKVAAPPLLEFFRHGGHDGAVGGEEAMETLKAAFQSVLASADLL 180

Query: 181 RRDTALKVTEILTPAQAVRFWAAVAQLHLRIRALGLQEDA 221
           RRDTALKVT+ILTPAQ VRF AAVAQLHLR+RALGLQEDA
Sbjct: 181 RRDTALKVTQILTPAQTVRFLAAVAQLHLRVRALGLQEDA 220

BLAST of Bhi11G001509 vs. ExPASy TrEMBL
Match: A0A1S3B5L4 (transcription factor HBP-1b(C38) OS=Cucumis melo OX=3656 GN=LOC103486278 PE=4 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 7.5e-105
Identity = 192/220 (87.27%), Postives = 204/220 (92.73%), Query Frame = 0

Query: 1   MPGDHYNADSFKAFFDAWLLRQRNFLDDLLSAESGSPHDGDLQDLISRVLSHYEDYYEKK 60
           M GDHYNADSFKAFF+AWLLRQRN+LDDLLSA  GSPH+ DLQ  ISR+LSHYEDYYEKK
Sbjct: 1   MAGDHYNADSFKAFFEAWLLRQRNYLDDLLSAAHGSPHNRDLQVSISRILSHYEDYYEKK 60

Query: 61  SRIAQTDIFLVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRRLRDD 120
           SRIAQTDIFLVF+PPWFTTYERTLLWIGGFRPGL  RLVNQSI+D+SDEQV+RIRRL+DD
Sbjct: 61  SRIAQTDIFLVFTPPWFTTYERTLLWIGGFRPGLIIRLVNQSIDDLSDEQVVRIRRLKDD 120

Query: 121 VKIEERLLNNDLAKIQEKVAAPPLLEFFRRGGHDGVIGGEEAMENLKAAFQSVLASADFF 180
            KIEERLLNNDLAKIQEKVAAPPLLEFFR GGHDG +GGEEAME LKAAFQSVLASAD  
Sbjct: 121 TKIEERLLNNDLAKIQEKVAAPPLLEFFRHGGHDGAVGGEEAMETLKAAFQSVLASADLL 180

Query: 181 RRDTALKVTEILTPAQAVRFWAAVAQLHLRIRALGLQEDA 221
           RRDTALKVT+ILTPAQ VRF AAVAQLHLR+RALGLQEDA
Sbjct: 181 RRDTALKVTQILTPAQTVRFLAAVAQLHLRVRALGLQEDA 220

BLAST of Bhi11G001509 vs. ExPASy TrEMBL
Match: A0A0A0LA62 (DOG1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G731050 PE=4 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 1.2e-102
Identity = 188/221 (85.07%), Postives = 204/221 (92.31%), Query Frame = 0

Query: 1   MPGDHYNADSFKAFFDAWLLRQRNFLDDLLSAESGSPHDGDLQDLISRVLSHYEDYYEKK 60
           M GDHYNADSFKAFF+AWLLRQRN+LDDLLS   G+P + DLQ  ISR+LSHYEDYYEKK
Sbjct: 1   MAGDHYNADSFKAFFEAWLLRQRNYLDDLLSTAHGTPQNRDLQVSISRILSHYEDYYEKK 60

Query: 61  SRIAQTDIFLVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRRLRDD 120
           SRIAQTDIFLVF+PPWFTTYE+TLLWIGGFRPGL  RLVNQSI+D+SDEQV+RIRRL+DD
Sbjct: 61  SRIAQTDIFLVFTPPWFTTYEKTLLWIGGFRPGLIVRLVNQSIDDLSDEQVVRIRRLKDD 120

Query: 121 VKIEERLLNNDLAKIQEKVAAPPLLEFFRRGGHDGVIGGEEAMENLKAAFQSVLASADFF 180
            K+EERLLNNDLAKIQEKVAAPPLLEFFR GGHDGVIGGE AME+LKAAFQSVLASAD  
Sbjct: 121 TKVEERLLNNDLAKIQEKVAAPPLLEFFRHGGHDGVIGGEAAMESLKAAFQSVLASADLL 180

Query: 181 RRDTALKVTEILTPAQAVRFWAAVAQLHLRIRALGLQEDAE 222
           RRDTALKVT+ILTPAQ VRF AAVAQLHLR+RALGLQEDA+
Sbjct: 181 RRDTALKVTQILTPAQTVRFLAAVAQLHLRVRALGLQEDAK 221

BLAST of Bhi11G001509 vs. ExPASy TrEMBL
Match: A0A6J1FYS6 (protein DOG1-like 4 OS=Cucurbita moschata OX=3662 GN=LOC111449039 PE=4 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 5.1e-93
Identity = 179/225 (79.56%), Postives = 195/225 (86.67%), Query Frame = 0

Query: 1   MPGDHYNADSFKAFFDAWLLRQRNFLDDLLSAESGSPHD----GDLQDLISRVLSHYEDY 60
           MPGD YN DSF AFF+ WLLRQ+ +LD+LL+A    P D    GDL DLISR LSHYE+Y
Sbjct: 1   MPGDDYNVDSFTAFFEGWLLRQQQYLDELLTATRRHPDDAADNGDLDDLISRTLSHYEEY 60

Query: 61  YEKKSRIAQTDIFLVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRR 120
           YEKKSRIAQ DIFLVFSP WFTTYE++LLWIGGFRPGL FRLVN+SIND+ D+QVLRI R
Sbjct: 61  YEKKSRIAQRDIFLVFSPTWFTTYEQSLLWIGGFRPGLIFRLVNESINDLWDDQVLRIGR 120

Query: 121 LRDDVKIEERLLNNDLAKIQEKVAAPPLLEFFRRGGHDGVIGGEEAMENLKAAFQSVLAS 180
           LR+DVK+EER+LNNDLAKIQEKVAAPPLLEFFRR GHDGV GG E ME LKAAFQSVLAS
Sbjct: 121 LREDVKVEERMLNNDLAKIQEKVAAPPLLEFFRRQGHDGVTGGTE-MEALKAAFQSVLAS 180

Query: 181 ADFFRRDTALKVTEILTPAQAVRFWAAVAQLHLRIRALGLQEDAE 222
           ADFFRR+TALKV EILTPAQ VRF AAVAQLHLRIRA GLQ+DAE
Sbjct: 181 ADFFRRETALKVAEILTPAQTVRFLAAVAQLHLRIRAYGLQKDAE 224

BLAST of Bhi11G001509 vs. ExPASy TrEMBL
Match: A0A6J1I255 (protein DOG1-like 4 OS=Cucurbita maxima OX=3661 GN=LOC111470210 PE=4 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 5.6e-92
Identity = 178/225 (79.11%), Postives = 193/225 (85.78%), Query Frame = 0

Query: 1   MPGDHYNADSFKAFFDAWLLRQRNFLDDLLSAESGSPHD----GDLQDLISRVLSHYEDY 60
           MPGD YN DSF AFF+ WLLRQ+ +LD+LL+A    P D     DL DLISR LSHYE+Y
Sbjct: 1   MPGDDYNVDSFTAFFEGWLLRQQQYLDELLTATRRHPDDAADNSDLDDLISRTLSHYEEY 60

Query: 61  YEKKSRIAQTDIFLVFSPPWFTTYERTLLWIGGFRPGLTFRLVNQSINDMSDEQVLRIRR 120
           YEKKSRIAQ DIFLVFSP WFTTYE++LLWIGGFRPGL FRLVN+SINDM D+QVLRI R
Sbjct: 61  YEKKSRIAQRDIFLVFSPTWFTTYEQSLLWIGGFRPGLIFRLVNESINDMWDDQVLRIGR 120

Query: 121 LRDDVKIEERLLNNDLAKIQEKVAAPPLLEFFRRGGHDGVIGGEEAMENLKAAFQSVLAS 180
           LR+DVK+EER+LNNDLAKIQEKVAAPPLLEFFRR GHDGV GG E ME LKAAFQSVLAS
Sbjct: 121 LREDVKVEERMLNNDLAKIQEKVAAPPLLEFFRRQGHDGVTGGTE-MEALKAAFQSVLAS 180

Query: 181 ADFFRRDTALKVTEILTPAQAVRFWAAVAQLHLRIRALGLQEDAE 222
           ADF RR+TALKV EILTPAQ VRF AAVAQLHLRIRA GLQ+DAE
Sbjct: 181 ADFLRRETALKVAEILTPAQTVRFLAAVAQLHLRIRACGLQKDAE 224

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G09950.11.1e-3638.07RESPONSE TO ABA AND SALT 1 [more]
AT1G58330.12.1e-3537.73transcription factor-related [more]
AT1G77920.15.1e-2129.76bZIP transcription factor family protein [more]
AT1G22070.11.1e-2029.27TGA1A-related gene 3 [more]
AT5G10030.11.6e-1929.76TGACG motif-binding factor 4 [more]
Match NameE-valueIdentityDescription
O045151.6e-3538.07Protein RESPONSE TO ABA AND SALT 1 OS=Arabidopsis thaliana OX=3702 GN=RAS1 PE=2 ... [more]
Q9SLV13.0e-3437.73Protein ZW2 OS=Arabidopsis thaliana OX=3702 GN=ZW2 PE=2 SV=1[more]
Q93ZE27.2e-2029.76Transcription factor TGA7 OS=Arabidopsis thaliana OX=3702 GN=TGA7 PE=1 SV=1[more]
Q392341.6e-1929.27Transcription factor TGA3 OS=Arabidopsis thaliana OX=3702 GN=TGA3 PE=1 SV=1[more]
Q0JNL34.7e-1927.40Transcription factor TGA2.3 OS=Oryza sativa subsp. japonica OX=39947 GN=TGA2.3 P... [more]
Match NameE-valueIdentityDescription
XP_038905753.15.3e-121100.00protein RESPONSE TO ABA AND SALT 1-like [Benincasa hispida][more]
XP_008442399.11.6e-10487.27PREDICTED: transcription factor HBP-1b(c38) [Cucumis melo] >KAA0044183.1 transcr... [more]
XP_031737579.12.5e-10285.07protein RESPONSE TO ABA AND SALT 1 [Cucumis sativus] >KGN58743.1 hypothetical pr... [more]
XP_022944640.11.0e-9279.56protein DOG1-like 4 [Cucurbita moschata][more]
KAG6596339.15.2e-9279.11Protein RESPONSE TO ABA AND SALT 1, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
A0A5A7TKZ87.5e-10587.27Transcription factor HBP-1b(C38) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3B5L47.5e-10587.27transcription factor HBP-1b(C38) OS=Cucumis melo OX=3656 GN=LOC103486278 PE=4 SV... [more]
A0A0A0LA621.2e-10285.07DOG1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G731050 PE=4 S... [more]
A0A6J1FYS65.1e-9379.56protein DOG1-like 4 OS=Cucurbita moschata OX=3662 GN=LOC111449039 PE=4 SV=1[more]
A0A6J1I2555.6e-9279.11protein DOG1-like 4 OS=Cucurbita maxima OX=3661 GN=LOC111470210 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 118..138
NoneNo IPR availablePANTHERPTHR46354FAMILY NOT NAMEDcoord: 7..221
NoneNo IPR availablePANTHERPTHR46354:SF13TRANSCRIPTION FACTOR HBP-1B(C38)-LIKE ISOFORM X1coord: 7..221
IPR025422Transcription factor TGA like domainPFAMPF14144DOG1coord: 28..100
e-value: 9.2E-24
score: 83.5
IPR025422Transcription factor TGA like domainPROSITEPS51806DOG1coord: 8..221
score: 30.234694

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi11M001509Bhi11M001509mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
molecular_function GO:0043565 sequence-specific DNA binding