Bhi10G001455 (gene) Wax gourd (B227) v1

Overview


Name	Bhi10G001455
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	RING-type domain-containing protein
Location	chr10: 45955144 .. 45956626 (-)
RNA-Seq Expression	Bhi10G001455
Synteny	Bhi10G001455

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCACTCCTCGGTGTTTACAGCCTCCCTTATTAAACTCCCATTTTAGAGAGAGAAACCCCCTCTTTCTCTCTCCTATTTTTTTTTTTAAATTATATATATATATATATATCCACACAGCATAGGAGAGGCAGTATCTTTTGTTATCATCCTTGGGAACATTATATGATTTCGTAAGAACAACCACTCTATGGATTCTTGAGTTCTTTCCTTTCTTCTTTGTTGTATGCAGAGCAGGCAAGGATTCCGCCCATTTGCGTTTTTGAAAGCGGGAACGCCGCGTAATTTCGTTTTCTTCTTTTTCTTTTGCCGTTTTGATTTCTGTTTTCGAACAATTACTCAACCACCACAAAAATGGCCATTCAATTGCAGTTACAGCGCCGGAATTTTGGCCTTCTTCCCTCCGATTCTACTCCTGCTGATCAGTTTCATCAATTATTTCATCCTAATCATCATCCTCTTCAACCAGGAAACGCTCAGTCCAGTTCTGACGATCTTCACACTCTCTCTTTCTTGCCGGAGATGTCGACGGATCAGATCGATCGCTTAATTCGATTGCAGGTCTTTACTTCGATTTCTCTCTGTGAATTAGTTATGTTGTTGATATTTTGTTCTTGAGTGTTTCGATTTCTCTACTGTAACAGAGTGAAAGATTCAGAGTACTTCTGCACCACAAAATAAATCAAGAAATCGGAGTGTTATTGAACCAGATCGAAACCAGAACGCGCGTTCTGTTTCAGCAGAAAGATGAAGAAATCGCTAATGCGAACGTGAGAAAAATCCATCTCGAACAATTATTGACGAGATTACAAATGGAGAATCAAGAACGGAAGAGAGCCGTACAAGAGAATCAAGCAATGGTAGCATCGCTTAGTCACGCACTCAATCAAATCAGAGAAAGGCTTTCATCGTGCGCGAACGATGCGGAGTCAAACAACAACAACAACAGAGACGGAGAAGACGACGCCATTGATTACGGAAAAAAGAGGAAGACGAAGAAGATGATGATGATTTGCCAAATCTGTAACTCTCGGATTTCGTGCGTGCTGTTGTTGCCTTGCAGGCACCTCTGTTCGTGTAAGCCATGCGAAAGTACTCTCGAGTTTTGCCCCGTCTGCAACACGACGAAGAAAGCCAGCATCGAGGCTGTGATTTTCTAGGCAAATTTTCCGGGAAAAAGTTGAAGTTGAAAACAAATATGAAAAGAAACCGTCTCACTTTTTCGGTAGGATTTTCGAATTTTATCCCTTTTTTCCTTCCTTTTTCCATTTTTTTTATTTTTCATTGATGAACACCAGTAATTGATTTTTTTTGGAAATCAAACTATACAGAAAAGAATCCAATAAACCTTGATGGATTATTCTT

mRNA sequence

ATGCACTCCTCGGTGTTTACAGCCTCCCTTATTAAACTCCCATTTTAGAGAGAGAAACCCCCTCTTTCTCTCTCCTATTTTTTTTTTTAAATTATATATATATATATATATCCACACAGCATAGGAGAGGCAGTATCTTTTGTTATCATCCTTGGGAACATTATATGATTTCGTAAGAACAACCACTCTATGGATTCTTGAGTTCTTTCCTTTCTTCTTTGTTGTATGCAGAGCAGGCAAGGATTCCGCCCATTTGCGTTTTTGAAAGCGGGAACGCCGCTTACAGCGCCGGAATTTTGGCCTTCTTCCCTCCGATTCTACTCCTGCTGATCAGTTTCATCAATTATTTCATCCTAATCATCATCCTCTTCAACCAGGAAACGCTCAGTCCAGTTCTGACGATCTTCACACTCTCTCTTTCTTGCCGGAGATGTCGACGGATCAGATCGATCGCTTAATTCGATTGCAGAGTGAAAGATTCAGAGTACTTCTGCACCACAAAATAAATCAAGAAATCGGAGTGTTATTGAACCAGATCGAAACCAGAACGCGCGTTCTGTTTCAGCAGAAAGATGAAGAAATCGCTAATGCGAACGTGAGAAAAATCCATCTCGAACAATTATTGACGAGATTACAAATGGAGAATCAAGAACGGAAGAGAGCCGTACAAGAGAATCAAGCAATGGTAGCATCGCTTAGTCACGCACTCAATCAAATCAGAGAAAGGCTTTCATCGTGCGCGAACGATGCGGAGTCAAACAACAACAACAACAGAGACGGAGAAGACGACGCCATTGATTACGGAAAAAAGAGGAAGACGAAGAAGATGATGATGATTTGCCAAATCTGTAACTCTCGGATTTCGTGCGTGCTGTTGTTGCCTTGCAGGCACCTCTGTTCGTGTAAGCCATGCGAAAGTACTCTCGAGTTTTGCCCCGTCTGCAACACGACGAAGAAAGCCAGCATCGAGGCTGTGATTTTCTAGGCAAATTTTCCGGGAAAAAGTTGAAGTTGAAAACAAATATGAAAAGAAACCGTCTCACTTTTTCGGTAGGATTTTCGAATTTTATCCCTTTTTTCCTTCCTTTTTCCATTTTTTTTATTTTTCATTGATGAACACCAGTAATTGATTTTTTTTGGAAATCAAACTATACAGAAAAGAATCCAATAAACCTTGATGGATTATTCTT

Coding sequence (CDS)

ATGTCGACGGATCAGATCGATCGCTTAATTCGATTGCAGAGTGAAAGATTCAGAGTACTTCTGCACCACAAAATAAATCAAGAAATCGGAGTGTTATTGAACCAGATCGAAACCAGAACGCGCGTTCTGTTTCAGCAGAAAGATGAAGAAATCGCTAATGCGAACGTGAGAAAAATCCATCTCGAACAATTATTGACGAGATTACAAATGGAGAATCAAGAACGGAAGAGAGCCGTACAAGAGAATCAAGCAATGGTAGCATCGCTTAGTCACGCACTCAATCAAATCAGAGAAAGGCTTTCATCGTGCGCGAACGATGCGGAGTCAAACAACAACAACAACAGAGACGGAGAAGACGACGCCATTGATTACGGAAAAAAGAGGAAGACGAAGAAGATGATGATGATTTGCCAAATCTGTAACTCTCGGATTTCGTGCGTGCTGTTGTTGCCTTGCAGGCACCTCTGTTCGTGTAAGCCATGCGAAAGTACTCTCGAGTTTTGCCCCGTCTGCAACACGACGAAGAAAGCCAGCATCGAGGCTGTGATTTTCTAG

Protein sequence

MSTDQIDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIHLEQLLTRLQMENQERKRAVQENQAMVASLSHALNQIRERLSSCANDAESNNNNNRDGEDDAIDYGKKRKTKKMMMICQICNSRISCVLLLPCRHLCSCKPCESTLEFCPVCNTTKKASIEAVIF

Homology

[Close]

BLAST of Bhi10G001455 vs. TAIR 10
Match: AT4G35070.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 122.1 bits (305), Expect = 4.7e-28
Identity = 75/186 (40.32%), Postives = 117/186 (62.90%), Query Frame = 0


Query: 5   QIDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIHLEQL 64
           +ID+ I++Q+ER R +L  +  +E+ ++L ++E++  +L  QK+EE++ A  + + LE L
Sbjct: 84  EIDQFIKIQNERLRYVLQEQRKREMEMILRKMESKALLLMSQKEEEMSKALNKNMELEDL 143

Query: 65  LTRLQMENQERKRAVQENQAMVASLSHALNQIRERLSSC--ANDAESNNNNN-RDGEDDA 124
           L +++MENQ  +R  +EN+A+V +L+  L Q+RER ++C  A +AE  +  +   GE D 
Sbjct: 144 LRKMEMENQTWQRMARENEAIVQTLNTTLEQVRERAATCYDAGEAEVEDEGSFCGGEGD- 203

Query: 125 IDYGKKRKTKKMMMICQICN---SRISCVLLLPCRHLCSCKPCESTLEFCPVCNTTKKAS 184
              G     KKM M    CN   + ++ VL LPCRHLC C  CE  L  CP+CNT KK+ 
Sbjct: 204 ---GNSLPAKKMKMSSCCCNCGSNGVTRVLFLPCRHLCCCMDCEEGLLLCPICNTPKKSR 263

[Close]

BLAST of Bhi10G001455 vs. TAIR 10
Match: AT4G35070.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 122.1 bits (305), Expect = 4.7e-28
Identity = 75/186 (40.32%), Postives = 117/186 (62.90%), Query Frame = 0


Query: 5   QIDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIHLEQL 64
           +ID+ I++Q+ER R +L  +  +E+ ++L ++E++  +L  QK+EE++ A  + + LE L
Sbjct: 29  EIDQFIKIQNERLRYVLQEQRKREMEMILRKMESKALLLMSQKEEEMSKALNKNMELEDL 88

Query: 65  LTRLQMENQERKRAVQENQAMVASLSHALNQIRERLSSC--ANDAESNNNNN-RDGEDDA 124
           L +++MENQ  +R  +EN+A+V +L+  L Q+RER ++C  A +AE  +  +   GE D 
Sbjct: 89  LRKMEMENQTWQRMARENEAIVQTLNTTLEQVRERAATCYDAGEAEVEDEGSFCGGEGD- 148

Query: 125 IDYGKKRKTKKMMMICQICN---SRISCVLLLPCRHLCSCKPCESTLEFCPVCNTTKKAS 184
              G     KKM M    CN   + ++ VL LPCRHLC C  CE  L  CP+CNT KK+ 
Sbjct: 149 ---GNSLPAKKMKMSSCCCNCGSNGVTRVLFLPCRHLCCCMDCEEGLLLCPICNTPKKSR 208

[Close]

BLAST of Bhi10G001455 vs. TAIR 10
Match: AT1G45976.1 (S-ribonuclease binding protein 1 )

HSP 1 Score: 80.9 bits (198), Expect = 1.2e-15
Identity = 51/179 (28.49%), Postives = 95/179 (53.07%), Query Frame = 0


Query: 6   IDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIHLEQLL 65
           IDR +++Q ++ R  +  KI +     ++ +E +     ++KDEE+   N +   LE  +
Sbjct: 150 IDRFLKIQGDQLRHAILDKIKRGQQKTVSLMEEKVVQKLREKDEELERINRKNKELEVRM 209

Query: 66  TRLQMENQERKRAVQENQAMVASLSHALNQI----RERLSSCANDAESNNNNNRDGEDDA 125
            +L ME +  ++  + N+ M+A+L++ L++     R+ +  C +    +  +  +G D++
Sbjct: 210 EQLTMEAEAWQQRAKYNENMIAALNYNLDRAQGRPRDSIEGCGDSEVDDTASCFNGRDNS 269

Query: 126 IDYGKKRKTKKMMMICQICNSRISCVLLLPCRHLCSCKPCESTLEFCPVCNTTKKASIE 181
                     K MM+C+ C  R  C+LLLPC H+C CK CE  L  CP+C ++K   +E
Sbjct: 270 ------NNNTKTMMMCRFCGVREMCMLLLPCNHMCLCKECERKLSSCPLCQSSKFLGME 322

[Close]

BLAST of Bhi10G001455 vs. TAIR 10
Match: AT3G12920.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 78.6 bits (192), Expect = 6.0e-15
Identity = 61/189 (32.28%), Postives = 96/189 (50.79%), Query Frame = 0


Query: 6   IDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIHLEQLL 65
           IDRLI    ER R+ +  K   +   ++  +E       + KD+EI +     + LE+ +
Sbjct: 141 IDRLISNHVERMRMEIEEKRKTQGRRIVEAVEQGLMKTLRAKDDEINHIGKLNLFLEEKV 200

Query: 66  TRLQMENQERKRAVQENQAMVASLSHALNQIR--------ERLSSCANDAESNNNNNRDG 125
             L +ENQ  +   Q N+A V +L   L Q+         E   + A+DA+S   +N +G
Sbjct: 201 KSLCVENQIWRDVAQSNEATVNALRSNLQQVLAAVERNRWEEPPTVADDAQSCCGSNDEG 260

Query: 126 EDDAIDY---GKKRKTKKM----MMICQICNSRISCVLLLPCRHLCSCKPCESTLEFCPV 180
           + +   +   G+ + TKKM    M +C+ C    + VLLLPCRH+C C  C S+L  CP+
Sbjct: 261 DSEEERWKLAGEAQDTKKMCRVGMSMCRSCGKGEASVLLLPCRHMCLCSVCGSSLNTCPI 320

[Close]

BLAST of Bhi10G001455 vs. TAIR 10
Match: AT1G79110.2 (zinc ion binding )

HSP 1 Score: 67.8 bits (164), Expect = 1.1e-11
Identity = 56/199 (28.14%), Postives = 88/199 (44.22%), Query Frame = 0


Query: 5   QIDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIHLEQL 64
           +IDR + L  ER +  +  K  ++   ++  IE       + K+EE          LE+ 
Sbjct: 155 EIDRFVSLHMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEER 214

Query: 65  LTRLQMENQERKRAVQENQA----MVASLSHALNQIRERL-------------------- 124
           +  L +ENQ  +   Q N+A    +  +L H L Q+++                      
Sbjct: 215 VKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMNEEDDAESCCG 274

Query: 125 SSCANDAESNNNNNRDGEDDAIDYGKKRKTKKMMMICQICNSRISCVLLLPCRHLCSCKP 180
           SSC    E         E +A D  ++R+ +    +C+ C    SCVLLLPCRHLC C  
Sbjct: 275 SSCGGGGEETVRRRVGLEREAQDKAERRRRR----MCRNCGEEESCVLLLPCRHLCLCGV 334

[Close]

BLAST of Bhi10G001455 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 8.4e-14
Identity = 61/189 (32.28%), Postives = 96/189 (50.79%), Query Frame = 0


Query: 6   IDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIHLEQLL 65
           IDRLI    ER R+ +  K   +   ++  +E       + KD+EI +     + LE+ +
Sbjct: 141 IDRLISNHVERMRMEIEEKRKTQGRRIVEAVEQGLMKTLRAKDDEINHIGKLNLFLEEKV 200

Query: 66  TRLQMENQERKRAVQENQAMVASLSHALNQIR--------ERLSSCANDAESNNNNNRDG 125
             L +ENQ  +   Q N+A V +L   L Q+         E   + A+DA+S   +N +G
Sbjct: 201 KSLCVENQIWRDVAQSNEATVNALRSNLQQVLAAVERNRWEEPPTVADDAQSCCGSNDEG 260

Query: 126 EDDAIDY---GKKRKTKKM----MMICQICNSRISCVLLLPCRHLCSCKPCESTLEFCPV 180
           + +   +   G+ + TKKM    M +C+ C    + VLLLPCRH+C C  C S+L  CP+
Sbjct: 261 DSEEERWKLAGEAQDTKKMCRVGMSMCRSCGKGEASVLLLPCRHMCLCSVCGSSLNTCPI 320

[Close]

BLAST of Bhi10G001455 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.6e-09
Identity = 57/202 (28.22%), Postives = 89/202 (44.06%), Query Frame = 0


Query: 5   QIDRLIRL---QSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIHL 64
           +IDR + L   Q ER +  +  K  ++   ++  IE       + K+EE          L
Sbjct: 155 EIDRFVSLHLYQMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHAL 214

Query: 65  EQLLTRLQMENQERKRAVQENQA----MVASLSHALNQIRERL----------------- 124
           E+ +  L +ENQ  +   Q N+A    +  +L H L Q+++                   
Sbjct: 215 EERVKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMNEEDDAES 274

Query: 125 ---SSCANDAESNNNNNRDGEDDAIDYGKKRKTKKMMMICQICNSRISCVLLLPCRHLCS 180
              SSC    E         E +A D  ++R+ +    +C+ C    SCVLLLPCRHLC 
Sbjct: 275 CCGSSCGGGGEETVRRRVGLEREAQDKAERRRRR----MCRNCGEEESCVLLLPCRHLCL 334

[Close]

BLAST of Bhi10G001455 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.4e-08
Identity = 55/187 (29.41%), Postives = 85/187 (45.45%), Query Frame = 0


Query: 5   QIDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIHLEQL 64
           +IDR +  Q+E  R+ L  +   +  +L + ++       + KDEEI         L++ 
Sbjct: 112 EIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGKLNWVLQER 171

Query: 65  LTRLQMENQERKRAVQENQAMVASLSHALNQI----------RERLSSCANDAESNNNNN 124
           +  L +ENQ  +   Q N+A   +L   L Q+          R  L   A+DAES+  + 
Sbjct: 172 VKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRPLVEEADDAESSCGSC 231

Query: 125 RDGEDDAIDYGKKRKTKKMMMICQICNSRISCVLLLPCRHLCSCKPCEST--LEFCPVCN 180
             G+  A+  G           C+ C    + VL+LPCRHLC C  C S+  L  CPVC+
Sbjct: 232 DGGDVTAVVNGG----------CKRCGELTASVLVLPCRHLCLCTVCGSSALLRTCPVCD 288

[Close]

BLAST of Bhi10G001455 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 7.6e-07
Identity = 45/177 (25.42%), Postives = 81/177 (45.76%), Query Frame = 0


Query: 5   QIDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIHLEQL 64
           +IDR +  Q+E+ R+ +  +   +  +L + ++       ++KD+EI         L++ 
Sbjct: 122 EIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAKKLKEKDDEIVRIRNLNWVLQER 181

Query: 65  LTRLQMENQERKRAVQENQAMVASLSHALNQIRERLSSCANDAESNNNNNRDGEDDAIDY 124
           +  L +ENQ  +   Q N+A   +L   L+Q+  +L +    +    ++           
Sbjct: 182 VKSLYVENQIWRDIAQTNEANANTLRTNLDQVLAQLETFPTASAVVEDDAESSCGSCCGD 241

Query: 125 GKKRKTKKMMMICQICNSRISCVLLLPCRHLCSCKPC--ESTLEFCPVCNTTKKASI 180
           G       +   C+ C  R + VL+LPCRHLC C  C   + L  CPVC+    AS+
Sbjct: 242 GGGEAVTAVGGGCKRCGEREASVLVLPCRHLCLCTVCGGSALLRTCPVCDMVMNASV 298

[Close]

BLAST of Bhi10G001455 vs. ExPASy Swiss-Prot
Match: Q9FW70 (Kinesin-like protein KIN-7K, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=KIN7K PE=2 SV=2)

HSP 1 Score: 47.4 bits (111), Expect = 2.1e-04
Identity = 17/38 (44.74%), Postives = 23/38 (60.53%), Query Frame = 0


Query: 136  ICQICNSRISCVLLLPCRHLCSCKPCESTLEFCPVCNT 174
            +C++C    +  +LLPCRH C CKPC      CP+C T
Sbjct: 1012 VCKVCFESATAAVLLPCRHFCLCKPCSLACSECPLCRT 1049

[Close]

BLAST of Bhi10G001455 vs. NCBI nr
Match: XP_038902052.1 (probable BOI-related E3 ubiquitin-protein ligase 3 [Benincasa hispida])

HSP 1 Score: 353.2 bits (905), Expect = 1.3e-93
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MSTDQIDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIH 60
           MSTDQIDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIH
Sbjct: 57  MSTDQIDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIH 116

Query: 61  LEQLLTRLQMENQERKRAVQENQAMVASLSHALNQIRERLSSCANDAESNNNNNRDGEDD 120
           LEQLLTRLQMENQERKRAVQENQAMVASLSHALNQIRERLSSCANDAESNNNNNRDGEDD
Sbjct: 117 LEQLLTRLQMENQERKRAVQENQAMVASLSHALNQIRERLSSCANDAESNNNNNRDGEDD 176

Query: 121 AIDYGKKRKTKKMMMICQICNSRISCVLLLPCRHLCSCKPCESTLEFCPVCNTTKKASIE 180
           AIDYGKKRKTKKMMMICQICNSRISCVLLLPCRHLCSCKPCESTLEFCPVCNTTKKASIE
Sbjct: 177 AIDYGKKRKTKKMMMICQICNSRISCVLLLPCRHLCSCKPCESTLEFCPVCNTTKKASIE 236

Query: 181 AVIF 185
           AVIF
Sbjct: 237 AVIF 240

[Close]

BLAST of Bhi10G001455 vs. NCBI nr
Match: XP_004147074.1 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X1 [Cucumis sativus] >KGN61937.1 hypothetical protein Csa_006158 [Cucumis sativus])

HSP 1 Score: 303.9 bits (777), Expect = 9.4e-79
Identity = 159/188 (84.57%), Postives = 173/188 (92.02%), Query Frame = 0


Query: 1   MSTDQIDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIH 60
           M  D IDR IRLQSERFR+LL  KINQ+IGVLLNQIETRTRVLFQQKDEEIA AN+R++H
Sbjct: 82  MPADHIDRFIRLQSERFRLLLQQKINQQIGVLLNQIETRTRVLFQQKDEEIACANMRRMH 141

Query: 61  LEQLLTRLQMENQERKRAVQENQAMVASLSHALNQIRERLSSCANDAESNNNNN----RD 120
           LEQLLTRLQMENQERK++VQENQAMVASLS ALNQIRE++S CANDAESNNNNN    R+
Sbjct: 142 LEQLLTRLQMENQERKKSVQENQAMVASLSRALNQIREKVSLCANDAESNNNNNSNNYRN 201

Query: 121 GEDDAIDYGKKRKTKKMMMICQICNSRISCVLLLPCRHLCSCKPCESTLEFCPVCNTTKK 180
           GEDDAIDYGKK+K KK MMIC+ICNSR+SCVLLLPCRHLCSCKPCESTL+FCPVCNTTKK
Sbjct: 202 GEDDAIDYGKKKKKKKKMMICKICNSRVSCVLLLPCRHLCSCKPCESTLDFCPVCNTTKK 261

Query: 181 ASIEAVIF 185
           ASIEAVIF
Sbjct: 262 ASIEAVIF 269

[Close]

BLAST of Bhi10G001455 vs. NCBI nr
Match: XP_008457700.1 (PREDICTED: probable BOI-related E3 ubiquitin-protein ligase 3 [Cucumis melo])

HSP 1 Score: 303.9 bits (777), Expect = 9.4e-79
Identity = 159/186 (85.48%), Postives = 173/186 (93.01%), Query Frame = 0


Query: 1   MSTDQIDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIH 60
           M +D IDR IRLQSERFR+LL  KINQ+IGVLLNQIETRTRVLFQQKDEEIA AN+R+IH
Sbjct: 88  MPSDHIDRFIRLQSERFRLLLQQKINQQIGVLLNQIETRTRVLFQQKDEEIACANMRRIH 147

Query: 61  LEQLLTRLQMENQERKRAVQENQAMVASLSHALNQIRERLSSCANDAESNNNNN--RDGE 120
           LEQLLTRLQMENQERK++VQENQAMVASLS ALNQIRE++S CANDAESNNNNN  R+GE
Sbjct: 148 LEQLLTRLQMENQERKKSVQENQAMVASLSRALNQIREKVSLCANDAESNNNNNNYRNGE 207

Query: 121 DDAIDYGKKRKTKKMMMICQICNSRISCVLLLPCRHLCSCKPCESTLEFCPVCNTTKKAS 180
           DDAIDYGK +K KK MMIC+ICNSR+SCVLLLPCRHLCSCKPCESTL+FCPVCNTTKKAS
Sbjct: 208 DDAIDYGKTKKKKKKMMICKICNSRVSCVLLLPCRHLCSCKPCESTLDFCPVCNTTKKAS 267

Query: 181 IEAVIF 185
           IEAVIF
Sbjct: 268 IEAVIF 273

[Close]

BLAST of Bhi10G001455 vs. NCBI nr
Match: XP_022943834.1 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Cucurbita moschata] >KAG7010849.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 274.2 bits (700), Expect = 7.9e-70
Identity = 144/183 (78.69%), Postives = 161/183 (87.98%), Query Frame = 0


Query: 1   MSTDQIDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIH 60
           MS DQIDR IRL+SERFR+LL HKINQ+I VLLNQIE RTRVLFQQKDEEIA+ N+R I 
Sbjct: 56  MSVDQIDRFIRLESERFRLLLQHKINQQIRVLLNQIENRTRVLFQQKDEEIASTNMRTIQ 115

Query: 61  LEQLLTRLQMENQERKRAVQENQAMVASLSHALNQIRERLSSCANDAESNNNNNRDGEDD 120
           LEQLL +L+MENQ+RKRAV+ENQAMVASLSHAL+++RE+L + ANDAES+NNN R GEDD
Sbjct: 116 LEQLLKKLEMENQKRKRAVEENQAMVASLSHALDEMREKLLASANDAESSNNNVRIGEDD 175

Query: 121 AIDYGKKRKTKKMMMICQICNSRISCVLLLPCRHLCSCKPCESTLEFCPVCNTTKKASIE 180
           A D+G  +K KKM MICQICNSRISCVLLLPCRHLCSCK C S LEFCPVCN TKKASIE
Sbjct: 176 ASDFGNNKKRKKMRMICQICNSRISCVLLLPCRHLCSCKSCASALEFCPVCNATKKASIE 235

Query: 181 AVI 184
           AVI
Sbjct: 236 AVI 238

[Close]

BLAST of Bhi10G001455 vs. NCBI nr
Match: XP_022943835.1 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 [Cucurbita moschata])

HSP 1 Score: 274.2 bits (700), Expect = 7.9e-70
Identity = 144/183 (78.69%), Postives = 161/183 (87.98%), Query Frame = 0


Query: 1   MSTDQIDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIH 60
           MS DQIDR IRL+SERFR+LL HKINQ+I VLLNQIE RTRVLFQQKDEEIA+ N+R I 
Sbjct: 1   MSVDQIDRFIRLESERFRLLLQHKINQQIRVLLNQIENRTRVLFQQKDEEIASTNMRTIQ 60

Query: 61  LEQLLTRLQMENQERKRAVQENQAMVASLSHALNQIRERLSSCANDAESNNNNNRDGEDD 120
           LEQLL +L+MENQ+RKRAV+ENQAMVASLSHAL+++RE+L + ANDAES+NNN R GEDD
Sbjct: 61  LEQLLKKLEMENQKRKRAVEENQAMVASLSHALDEMREKLLASANDAESSNNNVRIGEDD 120

Query: 121 AIDYGKKRKTKKMMMICQICNSRISCVLLLPCRHLCSCKPCESTLEFCPVCNTTKKASIE 180
           A D+G  +K KKM MICQICNSRISCVLLLPCRHLCSCK C S LEFCPVCN TKKASIE
Sbjct: 121 ASDFGNNKKRKKMRMICQICNSRISCVLLLPCRHLCSCKSCASALEFCPVCNATKKASIE 180

Query: 181 AVI 184
           AVI
Sbjct: 181 AVI 183

[Close]

BLAST of Bhi10G001455 vs. ExPASy TrEMBL
Match: A0A0A0LMD7 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G270790 PE=4 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 4.5e-79
Identity = 159/188 (84.57%), Postives = 173/188 (92.02%), Query Frame = 0


Query: 1   MSTDQIDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIH 60
           M  D IDR IRLQSERFR+LL  KINQ+IGVLLNQIETRTRVLFQQKDEEIA AN+R++H
Sbjct: 82  MPADHIDRFIRLQSERFRLLLQQKINQQIGVLLNQIETRTRVLFQQKDEEIACANMRRMH 141

Query: 61  LEQLLTRLQMENQERKRAVQENQAMVASLSHALNQIRERLSSCANDAESNNNNN----RD 120
           LEQLLTRLQMENQERK++VQENQAMVASLS ALNQIRE++S CANDAESNNNNN    R+
Sbjct: 142 LEQLLTRLQMENQERKKSVQENQAMVASLSRALNQIREKVSLCANDAESNNNNNSNNYRN 201

Query: 121 GEDDAIDYGKKRKTKKMMMICQICNSRISCVLLLPCRHLCSCKPCESTLEFCPVCNTTKK 180
           GEDDAIDYGKK+K KK MMIC+ICNSR+SCVLLLPCRHLCSCKPCESTL+FCPVCNTTKK
Sbjct: 202 GEDDAIDYGKKKKKKKKMMICKICNSRVSCVLLLPCRHLCSCKPCESTLDFCPVCNTTKK 261

Query: 181 ASIEAVIF 185
           ASIEAVIF
Sbjct: 262 ASIEAVIF 269

[Close]

BLAST of Bhi10G001455 vs. ExPASy TrEMBL
Match: A0A1S3C6S8 (probable BOI-related E3 ubiquitin-protein ligase 3 OS=Cucumis melo OX=3656 GN=LOC103497335 PE=4 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 4.5e-79
Identity = 159/186 (85.48%), Postives = 173/186 (93.01%), Query Frame = 0


Query: 1   MSTDQIDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIH 60
           M +D IDR IRLQSERFR+LL  KINQ+IGVLLNQIETRTRVLFQQKDEEIA AN+R+IH
Sbjct: 88  MPSDHIDRFIRLQSERFRLLLQQKINQQIGVLLNQIETRTRVLFQQKDEEIACANMRRIH 147

Query: 61  LEQLLTRLQMENQERKRAVQENQAMVASLSHALNQIRERLSSCANDAESNNNNN--RDGE 120
           LEQLLTRLQMENQERK++VQENQAMVASLS ALNQIRE++S CANDAESNNNNN  R+GE
Sbjct: 148 LEQLLTRLQMENQERKKSVQENQAMVASLSRALNQIREKVSLCANDAESNNNNNNYRNGE 207

Query: 121 DDAIDYGKKRKTKKMMMICQICNSRISCVLLLPCRHLCSCKPCESTLEFCPVCNTTKKAS 180
           DDAIDYGK +K KK MMIC+ICNSR+SCVLLLPCRHLCSCKPCESTL+FCPVCNTTKKAS
Sbjct: 208 DDAIDYGKTKKKKKKMMICKICNSRVSCVLLLPCRHLCSCKPCESTLDFCPVCNTTKKAS 267

Query: 181 IEAVIF 185
           IEAVIF
Sbjct: 268 IEAVIF 273

[Close]

BLAST of Bhi10G001455 vs. ExPASy TrEMBL
Match: A0A6J1FVE9 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448450 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.8e-70
Identity = 144/183 (78.69%), Postives = 161/183 (87.98%), Query Frame = 0


Query: 1   MSTDQIDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIH 60
           MS DQIDR IRL+SERFR+LL HKINQ+I VLLNQIE RTRVLFQQKDEEIA+ N+R I 
Sbjct: 56  MSVDQIDRFIRLESERFRLLLQHKINQQIRVLLNQIENRTRVLFQQKDEEIASTNMRTIQ 115

Query: 61  LEQLLTRLQMENQERKRAVQENQAMVASLSHALNQIRERLSSCANDAESNNNNNRDGEDD 120
           LEQLL +L+MENQ+RKRAV+ENQAMVASLSHAL+++RE+L + ANDAES+NNN R GEDD
Sbjct: 116 LEQLLKKLEMENQKRKRAVEENQAMVASLSHALDEMREKLLASANDAESSNNNVRIGEDD 175

Query: 121 AIDYGKKRKTKKMMMICQICNSRISCVLLLPCRHLCSCKPCESTLEFCPVCNTTKKASIE 180
           A D+G  +K KKM MICQICNSRISCVLLLPCRHLCSCK C S LEFCPVCN TKKASIE
Sbjct: 176 ASDFGNNKKRKKMRMICQICNSRISCVLLLPCRHLCSCKSCASALEFCPVCNATKKASIE 235

Query: 181 AVI 184
           AVI
Sbjct: 236 AVI 238

[Close]

BLAST of Bhi10G001455 vs. ExPASy TrEMBL
Match: A0A6J1FYB4 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448450 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.8e-70
Identity = 144/183 (78.69%), Postives = 161/183 (87.98%), Query Frame = 0


Query: 1   MSTDQIDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIH 60
           MS DQIDR IRL+SERFR+LL HKINQ+I VLLNQIE RTRVLFQQKDEEIA+ N+R I 
Sbjct: 1   MSVDQIDRFIRLESERFRLLLQHKINQQIRVLLNQIENRTRVLFQQKDEEIASTNMRTIQ 60

Query: 61  LEQLLTRLQMENQERKRAVQENQAMVASLSHALNQIRERLSSCANDAESNNNNNRDGEDD 120
           LEQLL +L+MENQ+RKRAV+ENQAMVASLSHAL+++RE+L + ANDAES+NNN R GEDD
Sbjct: 61  LEQLLKKLEMENQKRKRAVEENQAMVASLSHALDEMREKLLASANDAESSNNNVRIGEDD 120

Query: 121 AIDYGKKRKTKKMMMICQICNSRISCVLLLPCRHLCSCKPCESTLEFCPVCNTTKKASIE 180
           A D+G  +K KKM MICQICNSRISCVLLLPCRHLCSCK C S LEFCPVCN TKKASIE
Sbjct: 121 ASDFGNNKKRKKMRMICQICNSRISCVLLLPCRHLCSCKSCASALEFCPVCNATKKASIE 180

Query: 181 AVI 184
           AVI
Sbjct: 181 AVI 183

[Close]

BLAST of Bhi10G001455 vs. ExPASy TrEMBL
Match: A0A6J1JA52 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483947 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 3.3e-69
Identity = 143/183 (78.14%), Postives = 160/183 (87.43%), Query Frame = 0


Query: 1   MSTDQIDRLIRLQSERFRVLLHHKINQEIGVLLNQIETRTRVLFQQKDEEIANANVRKIH 60
           MS DQIDR IRL+SERFR+LL HKINQ+I VLLNQIE RTRVLFQQKDEEIA+ N+R I 
Sbjct: 1   MSVDQIDRFIRLESERFRLLLQHKINQQIRVLLNQIENRTRVLFQQKDEEIASTNMRTIQ 60

Query: 61  LEQLLTRLQMENQERKRAVQENQAMVASLSHALNQIRERLSSCANDAESNNNNNRDGEDD 120
           LEQLL +L+MENQ RKRAV+ENQAMVASLS AL+++RE+L + ANDAES+NNN R GEDD
Sbjct: 61  LEQLLKKLEMENQMRKRAVEENQAMVASLSQALDEMREKLLASANDAESSNNNVRIGEDD 120

Query: 121 AIDYGKKRKTKKMMMICQICNSRISCVLLLPCRHLCSCKPCESTLEFCPVCNTTKKASIE 180
           A D+G  +K KKMM+ICQICNSRISCVLLLPCRHLCSCK C S LEFCPVCN TKKASIE
Sbjct: 121 ASDFGNNKKRKKMMLICQICNSRISCVLLLPCRHLCSCKSCASALEFCPVCNATKKASIE 180

Query: 181 AVI 184
           AVI
Sbjct: 181 AVI 183

The following BLAST results are available for this feature:

BLAST of Bhi10G001455 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35070.1	4.7e-28	40.32	SBP (S-ribonuclease binding protein) family protein	[more]
AT4G35070.2	4.7e-28	40.32	SBP (S-ribonuclease binding protein) family protein	[more]
AT1G45976.1	1.2e-15	28.49	S-ribonuclease binding protein 1	[more]
AT3G12920.1	6.0e-15	32.28	SBP (S-ribonuclease binding protein) family protein	[more]
AT1G79110.2	1.1e-11	28.14	zinc ion binding	[more]

BLAST of Bhi10G001455 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LDD1	8.4e-14	32.28	Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37...	[more]
F4IDI6	1.6e-09	28.22	Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37...	[more]
Q9FHE4	2.4e-08	29.41	BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG...	[more]
O81851	7.6e-07	25.42	E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1	[more]
Q9FW70	2.1e-04	44.74	Kinesin-like protein KIN-7K, chloroplastic OS=Oryza sativa subsp. japonica OX=39...	[more]

BLAST of Bhi10G001455 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038902052.1	1.3e-93	100.00	probable BOI-related E3 ubiquitin-protein ligase 3 [Benincasa hispida]	[more]
XP_004147074.1	9.4e-79	84.57	probable BOI-related E3 ubiquitin-protein ligase 3 isoform X1 [Cucumis sativus] ...	[more]
XP_008457700.1	9.4e-79	85.48	PREDICTED: probable BOI-related E3 ubiquitin-protein ligase 3 [Cucumis melo]	[more]
XP_022943834.1	7.9e-70	78.69	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Cucurbita moschat...	[more]
XP_022943835.1	7.9e-70	78.69	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 [Cucurbita moschat...	[more]

BLAST of Bhi10G001455 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LMD7	4.5e-79	84.57	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G270790 P...	[more]
A0A1S3C6S8	4.5e-79	85.48	probable BOI-related E3 ubiquitin-protein ligase 3 OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A6J1FVE9	3.8e-70	78.69	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita mosch...	[more]
A0A6J1FYB4	3.8e-70	78.69	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 OS=Cucurbita mosch...	[more]
A0A6J1JA52	3.3e-69	78.14	probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita maxim...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 51..81
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 136..174 e-value: 6.0E-8 score: 32.4
None	No IPR available	PANTHER	PTHR42647:SF6	BOI-RELATED E3 UBIQUITIN-PROTEIN LIGASE 2-RELATED	coord: 4..183
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 106..182 e-value: 1.8E-10 score: 42.1
IPR017066	E3 ubiquitin-protein ligase BOI-like	PIRSF	PIRSF036836	SBP1_RNase_bind	coord: 2..184 e-value: 4.9E-40 score: 136.0
IPR017066	E3 ubiquitin-protein ligase BOI-like	PANTHER	PTHR42647	SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEIN	coord: 4..183
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 137..172 score: 9.74522

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi10M001455	Bhi10M001455	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity