Bhi10G001169 (gene) Wax gourd (B227) v1

BLAST of Bhi10G001169 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 508.1 bits (1307), Expect = 8.4e-144
Identity = 258/506 (50.99%), Postives = 356/506 (70.36%), Query Frame = 0

Query: 5   LLTWILLSLVCLLLGYSFLPKPNRLNLPPTPLFCLPFIGHLHLLKHPVHRTFQNLSQKY- 64
           ++ + + SL+ L++ + FL KP + NLPP+P   LP IGHL LLK P+HRT ++ S+   
Sbjct: 8   VVLYSIFSLIFLIISFKFL-KPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLD 67

Query: 65  ----GRVFSLKFGSRLVVIVSSPS-AVQECFTKNDIILANRPYLETGKYLAYNNTTLVVS 124
               G V SL+ GSRLV +VSS   A +ECF KND++LANRP +  GK++ YNNT ++ +
Sbjct: 68  HNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAA 127

Query: 125 PYGEHWRNLRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNNHGLEDEFRVVELEPM 184
           PYG+HWRNLRR+ T+EIFST RLN F  +R  EV+RL+ +L   +     +  VVEL+PM
Sbjct: 128 PYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRL---SRLAGTKKTVVELKPM 187

Query: 185 LLDLTFNIVMRMVGGNKFCEENNNVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWGR 244
           L+DLTFN +MRM+ G ++  E      DE  + + R+++  +     S N  D++P+   
Sbjct: 188 LMDLTFNNIMRMMTGKRYYGEETT---DEEEAKRVRKLVADVGANTSSGNAVDYVPILRL 247

Query: 245 IDPTDFNKKLDKLGRRADELLQELVDEIRNQEDDGNTMIHHLLRLQNTDPEYYSDQVIKG 304
              + +  ++ KLG   D+ LQ L+D+ R Q++ G TMI HLL LQ +D EYY+DQ+IKG
Sbjct: 248 F--SSYENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKG 307

Query: 305 LVQVVFLAGVDTAAVTLEWALSELLNNPEVLKKARDEIDSVIGQEGLVNEADLSSLNYLQ 364
           ++ ++ +AG +T+AVTLEWALS LLN+P+V+ KARDEID+ +G + L+ EADLS L YL+
Sbjct: 308 IILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLK 367

Query: 365 GIITETLRLNPAAPLLVPHCASEDCKIGGYDVPRDTIVLINAWAIHRDLSLWEDAASFKP 424
            I+ ETLRL+PA PLLVPH ASEDCKIG YD+PR T +L+NAWAIHRD + W+D  SFKP
Sbjct: 368 NIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKP 427

Query: 425 ERYENAVGVDSCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSNSLVDMTEG 484
           ER+E     ++ KL+ FGLGRRACPG G+AQR++ L L SLIQCFEW+R+ N  VDM EG
Sbjct: 428 ERFEKE--EEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEG 487

Query: 485 EGLTMPKAQPLIAKCRPRPIMKEMLS 505
            G T+PKA PL A C+ RP + +++S
Sbjct: 488 VGNTVPKAIPLKAICKARPFLHKIIS 502

BLAST of Bhi10G001169 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 500.0 bits (1286), Expect = 2.3e-141
Identity = 254/494 (51.42%), Postives = 352/494 (71.26%), Query Frame = 0

Query: 6   LTWILLSLVCLLLGYSFLPKPNRLNLPPTPLFCLPFIGHLHLLKHPVHRTFQNLSQKYGR 65
           + +++LSL+ L+      P     NLPP+P + LP IGHL LLK P+HRTF +LSQ    
Sbjct: 10  ILFVVLSLIYLIGKLKRKP-----NLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNN 69

Query: 66  --VFSLKFGSRLVVIVSSPSAVQECFTKNDIILANRPYLETGKYLAYNNTTLVVSPYGEH 125
             +FSL+ G+RLV + SS S  +ECFTKND++LANRP     K++AY+ TT++ + YG+H
Sbjct: 70  APIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDH 129

Query: 126 WRNLRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNNHGLEDEFRVVELEPMLLDLT 185
           WRNLRRI ++EIFS  RLNSF  IR+ E++RL+ +L  N      EF  V+++ ML DLT
Sbjct: 130 WRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRN---FSQEFVKVDMKSMLSDLT 189

Query: 186 FNIVMRMVGGNKFCEENNNVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWGRIDPTD 245
           FN ++RMV G ++  +    +ED+  + + R++I  ++    + N  D++P+   +  +D
Sbjct: 190 FNNILRMVAGKRYYGDG---VEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SD 249

Query: 246 FNKKLDKLGRRADELLQELVDEIRNQEDDGNTMIHHLLRLQNTDPEYYSDQVIKGLVQVV 305
           +  ++ KL  R DE LQ LVDE R  ++ GNTMI HLL LQ + P+Y++D++IKG +  +
Sbjct: 250 YETRVKKLAGRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLAL 309

Query: 306 FLAGVDTAAVTLEWALSELLNNPEVLKKARDEIDSVIGQEGLVNEADLSSLNYLQGIITE 365
            LAG DT+AVTLEWALS +LN+P+VL KARDEID  IG + L++E+D+S+L YLQ I++E
Sbjct: 310 ILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSE 369

Query: 366 TLRLNPAAPLLVPHCASEDCKIGGYDVPRDTIVLINAWAIHRDLSLWEDAASFKPERYEN 425
           TLRL PAAP+L+PH ASEDCK+ GYD+PR TI+L N WAIHRD  LW+D  SFKPER+E 
Sbjct: 370 TLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEK 429

Query: 426 AVGVDSCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSNSLVDMTEGEGLTM 485
               ++ KL+PFGLGRRACPG G+A R++ LTL SLIQC EW+++    VDM+EG+G+TM
Sbjct: 430 E--GEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTM 487

Query: 486 PKAQPLIAKCRPRP 498
           PKA+PL A CR RP
Sbjct: 490 PKAKPLEAMCRARP 487

BLAST of Bhi10G001169 vs. TAIR 10
Match: AT3G28740.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 496.1 bits (1276), Expect = 3.3e-140
Identity = 258/504 (51.19%), Postives = 350/504 (69.44%), Query Frame = 0

Query: 8   WILLSL----VCLLLGYSFLPKPNRLNLPPTPLFCLPFIGHLHLLKHPVHRTFQNLSQKY 67
           +++LSL    + L L   F P+P +LNLPP+P    P IGHLHLLK P+HR F +LS+  
Sbjct: 12  YLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSESL 71

Query: 68  --GRVFSLKFGSRLVVIVSSPSAVQECFTKNDIILANRPYLETGKYLAYNNTTLVVSPYG 127
              ++FSL  GSRLV +VSS +  +ECFTKND++LANRP    GK++ YN+TT+V + YG
Sbjct: 72  NNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAYG 131

Query: 128 EHWRNLRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNN-HGLEDEFRVVELEPMLL 187
           + WRNLRRI T+EIFS+ RLNSF  IR+ E++RL+  L  N+ HG    F  VE++P+ +
Sbjct: 132 DSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHG----FVKVEMKPLFM 191

Query: 188 DLTFNIVMRMVGGNKFCEENNNVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWGRID 247
            LT N ++RMV G +F  +     E++  +   R++I +++  G + N  D+ P+   + 
Sbjct: 192 CLTINNIIRMVAGKRFYGDGT---ENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV- 251

Query: 248 PTDFNKKLDKLGRRADELLQELVDEIRNQEDDGNTMIHHLLRLQNTDPEYYSDQVIKGLV 307
            T++ K + KL  R DE LQ LV+E R ++  GNTMI HLL LQ T P+YY+D +IKG++
Sbjct: 252 -TNYEKHVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKGII 311

Query: 308 QVVFLAGVDTAAVTLEWALSELLNNPEVLKKARDEIDSVIGQEGLVNEADLSSLNYLQGI 367
            V+ LAG DT+A TLEWA+S LLN+PEVL+KA+ EID  IG + LV E D+  L YLQ I
Sbjct: 312 LVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHI 371

Query: 368 ITETLRLNPAAPLLVPHCASEDCKIGGYDVPRDTIVLINAWAIHRDLSLWEDAASFKPER 427
           ++ETLRL P AP+L+PH ASEDC + GYDVPR TI+L+NAWAIHRD  LWE+   FKPER
Sbjct: 372 VSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPER 431

Query: 428 YENAVGVDSCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSNSLVDMTEGE- 487
           +E   G D  KL+PFG+GRR+CPG G+AQR++ L L SL+QCFEW+R+    +DM E E 
Sbjct: 432 FEKK-GEDK-KLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEK 491

Query: 488 GLTMPKAQPLIAKCRPRPIMKEML 504
           G TM KA  L A C+ RPI+ ++L
Sbjct: 492 GTTMRKATSLQAMCKARPIVHKVL 504

BLAST of Bhi10G001169 vs. TAIR 10
Match: AT4G37310.1 (cytochrome P450, family 81, subfamily H, polypeptide 1 )

HSP 1 Score: 495.7 bits (1275), Expect = 4.3e-140
Identity = 260/513 (50.68%), Postives = 349/513 (68.03%), Query Frame = 0

Query: 4   DLLTWILLSLVCLLLGYSFL--PKPNRLNLPPTPLFCLPFIGHLHLLKHP-VHRTFQNLS 63
           D +  IL +LV + +    L   KPN+ NLPP+P  C P IGHLHLLK P +HRT  +LS
Sbjct: 2   DCILLILTTLVAIFIVKIVLLVTKPNK-NLPPSPNICFPIIGHLHLLKKPLLHRTLSHLS 61

Query: 64  QKYGRVFSLKFGSRLVVIVSSPSAVQECF-TKNDIILANRPYLETGKYLAYNNTTLVVSP 123
              G VFSL+ GSRL VI+SSP+A +ECF TKNDI+LANRP    GKY+AY+ T++V +P
Sbjct: 62  HSLGPVFSLRLGSRLAVIISSPTAAEECFLTKNDIVLANRPRFIMGKYVAYDYTSMVTAP 121

Query: 124 YGEHWRNLRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNNHGLEDEFRV-VELEPM 183
           YG+HWRNLRRI+ LE+FST RLN+ + IR  EVK LL+KL    H L  E    VEL  +
Sbjct: 122 YGDHWRNLRRITALEVFSTNRLNASAEIRHDEVKMLLQKL----HDLSVERPAKVELRQL 181

Query: 184 LLDLTFNIVMRMVGGNKFCEENNNVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWGR 243
           L  LT N++MRM+ G +F EE++      G S +FRE++ ++++   + NP DF+P    
Sbjct: 182 LTGLTLNVIMRMMTGKRFFEEDDG--GKAGISLEFRELVAEILELSAADNPADFLPALRW 241

Query: 244 IDPTDFNKKLDKLGRRADELLQELVDEIRNQEDD---GNTMIHHLLRLQNTDPEYYSDQV 303
            D     K+  ++G R D LLQ  +DE R  +D     NTMI HLL  Q  +P  YSDQ 
Sbjct: 242 FDYKGLVKRAKRIGERMDSLLQGFLDEHRANKDRLEFKNTMIAHLLDSQEKEPHNYSDQT 301

Query: 304 IKGLVQVVFLAGVDTAAVTLEWALSELLNNPEVLKKARDEIDSVI---GQEGLVNEADLS 363
           IKGL+ ++ + G DT+A+T+EWA+S LLN+P++L+  R  ID+ +       L+ E DL 
Sbjct: 302 IKGLILMMVVGGTDTSALTVEWAMSNLLNHPQILETTRQNIDTQMETSSSRRLLKEEDLV 361

Query: 364 SLNYLQGIITETLRLNPAAPLLVPHCASEDCKIGGYDVPRDTIVLINAWAIHRDLSLWED 423
           ++NYL+ +++ETLRL P APL+VPH  S DC IGG++VPRDTIVL+N WAIHRD S+W+D
Sbjct: 362 NMNYLKNVVSETLRLYPVAPLMVPHVPSSDCVIGGFNVPRDTIVLVNLWAIHRDPSVWDD 421

Query: 424 AASFKPERYENA--VGVDSCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSN 483
             SFKPER+E +   G  + K++PFGLGRRACPG+ +A RV+ L L S+IQCFEW+  S 
Sbjct: 422 PTSFKPERFEGSDQFGHYNGKMMPFGLGRRACPGLSLANRVVGLLLGSMIQCFEWESGSG 481

Query: 484 SLVDMTEGEGLTMPKAQPLIAKCRPRPIMKEML 504
             VDMTEG GL++PKA+PL+  CR R +  E+L
Sbjct: 482 GQVDMTEGPGLSLPKAEPLVVTCRTREMASELL 507

BLAST of Bhi10G001169 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 494.2 bits (1271), Expect = 1.3e-139
Identity = 262/499 (52.51%), Postives = 343/499 (68.74%), Query Frame = 0

Query: 8   WILLSLVCLLLGYSFLPKPNRLNLPPTPLFCLPFIGHLHLLKHPVHRTFQNLSQKYG--R 67
           +I+LSL+ L+          +LNLPP+P + LP IGHL LLK P+HR F ++SQ  G   
Sbjct: 12  FIVLSLIFLIGRIK-----RKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAP 71

Query: 68  VFSLKFGSRLVVIVSSPSAVQECFTKNDIILANRPYLETGKYLAYNNTTLVVSPYGEHWR 127
           + SL+ G+RL+ +VSS S  +ECFTKND+ILANR    + K+++Y N+T+V + Y EHWR
Sbjct: 72  IISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWR 131

Query: 128 NLRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNNHGLEDEFRVVELEPMLLDLTFN 187
           NLRRI  LEIFS  RLNSFS IR  E++RL+ +L  N+      F  VE++ M  DLTFN
Sbjct: 132 NLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNS---SYGFTKVEMKSMFSDLTFN 191

Query: 188 IVMRMVGGNKFCEENNNVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWGRIDPTDFN 247
            ++RM+ G  +  +     ED+  + + R +I + M      N  D+IP+   I  T   
Sbjct: 192 NIIRMLAGKCYYGDGK---EDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWI--TYSE 251

Query: 248 KKLDKLGRRADELLQELVDEIR-NQEDDGNTMIHHLLRLQNTDPEYYSDQVIKGLVQVVF 307
            ++ KL  R DE LQ LVDE R  +E   NTM+ HLL LQ T PEYY D++IKG +  + 
Sbjct: 252 TRIKKLAGRLDEFLQGLVDEKREGKEKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLI 311

Query: 308 LAGVDTAAVTLEWALSELLNNPEVLKKARDEIDSVIGQEGLVNEADLSSLNYLQGIITET 367
             G DT AVTLEWALS LLNNPEVL KARDEID +IG + L+ E+D+ +L YLQ I++ET
Sbjct: 312 AGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSET 371

Query: 368 LRLNPAAPLLVPHCASEDCKIGGYDVPRDTIVLINAWAIHRDLSLWEDAASFKPERYENA 427
           LRL PAAP+L+PH AS+DCK+GGYD+PR T++L NAWAIHRD  LW+D  SFKPER+E  
Sbjct: 372 LRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEKE 431

Query: 428 VGVDSCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSNSLVDMTEGEGLTMP 487
              ++ KL+PFGLGRRACPG G+AQR++ L+L SLIQCFEW+R+    VDMTEG GLTMP
Sbjct: 432 --GEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMP 491

Query: 488 KAQPLIAKCRPRPIMKEML 504
           KA+PL A CR R  + ++L
Sbjct: 492 KARPLEAMCRARDFVGKIL 495

BLAST of Bhi10G001169 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.1e-145
Identity = 258/505 (51.09%), Postives = 355/505 (70.30%), Query Frame = 0

Query: 1   MDFDLLTWILLSLVCLLLGYSFLP-KPNRLNLPPTPLFCLPFIGHLHLLKHPVHRTFQNL 60
           M+   L +  L++V L +     P    R NLPP+P   LP IGHLHL+   +HR+  +L
Sbjct: 1   MESSTLLYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDL 60

Query: 61  SQKYGRVFSLKFGSRLVVIVSSPSAVQECFTKNDIILANRPYLETGKYLAYNNTTLVVSP 120
           S+KYG VFSL+ G+RLV++VSSP+A +ECFTKNDI+ ANRP    GKY+ YN TT+V SP
Sbjct: 61  SKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSP 120

Query: 121 YGEHWRNLRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNNHGLEDEFRVVELEPML 180
           YGEHWRNLRR++ +EIFS   LN F  IRE EVK+LL  L  ++     +F  VE++  L
Sbjct: 121 YGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSS---GQDFGKVEMKSKL 180

Query: 181 LDLTFNIVMRMVGGNKFCEENNNVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWGRI 240
            +L+FN+ MRMV G ++  ++     D   +  FR +I ++ ++  ++NPGDF+P    I
Sbjct: 181 SELSFNVTMRMVAGKRYFGQD----VDSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWI 240

Query: 241 DPTDFNKKLDKLGRRADELLQELVDEIRNQEDDGNTMIHHLLRLQNTDPEYYSDQVIKGL 300
           D  ++ KK+ K+ +  D  LQ L+ E R  +++  TMI HLL LQ + PEYY+DQ+IKG+
Sbjct: 241 DFKNYEKKVSKISQEMDAFLQRLIHESRINKNN-VTMIDHLLSLQESQPEYYTDQIIKGI 300

Query: 301 VQVVFLAGVDTAAVTLEWALSELLNNPEVLKKARDEIDSVIGQEGLVNEADLSSLNYLQG 360
           + V+ LAG DT+AVT+EWA+S LLN+PE L+KAR EI++ +G   L+ E DL  L YL  
Sbjct: 301 IMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHN 360

Query: 361 IITETLRLNPAAPLLVPHCASEDCKIGGYDVPRDTIVLINAWAIHRDLSLWEDAASFKPE 420
           II+ET RL PAAP+LVPH +S+DCK+ GYDVP+ TI+L+NAWAIHRD   W++   FKPE
Sbjct: 361 IISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPE 420

Query: 421 RYENAVGVDSCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSNSLVDMTEGE 480
           R+   V ++  KL+PFG+GRR+CPG G+AQRV+ LTL +LIQCFEW+R+  + +DM EG 
Sbjct: 421 RH-GGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGS 480

Query: 481 GLTMPKAQPLIAKCRPRPIMKEMLS 505
           GLTMPKAQPL A C+PR I+ +++S
Sbjct: 481 GLTMPKAQPLEALCKPRNILHKVVS 496

BLAST of Bhi10G001169 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 1.4e-143
Identity = 256/475 (53.89%), Postives = 337/475 (70.95%), Query Frame = 0

Query: 30  NLPPTPLFCLPFIGHLHLLKHPVHRTFQNLSQKYGRVFSLKFGSRLVVIVSSPSAVQECF 89
           NLPP P   +P IG+LH LKHP+HRTF  LSQ YG +FSL FGSRLVV+VSSPS   ECF
Sbjct: 29  NLPPGPP-TIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWFGSRLVVVVSSPSLAHECF 88

Query: 90  TKNDIILANRPYLETGKYLAYNNTTLVVSPYGEHWRNLRRISTLEIFSTTRLNSFSRIRE 149
           TKNDIILANRP   TGKY+ YN TTL  + YG+HWRNLRRI+T+++ S  RLNSF  +R 
Sbjct: 89  TKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNNRLNSFLGVRR 148

Query: 150 HEVKRLLRKLCGNNHGLEDEFRVVELEPMLLDLTFNIVMRMVGGNKFCEENNNVLEDEGY 209
            E  RL++KL  +       F  VEL P L ++TFN +MRM+ G ++  ++ +V  D   
Sbjct: 149 DETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMMRMISGKRYYGDDGDV-SDVEE 208

Query: 210 SNKFREMITQMMKYGVSTNPGDFIPLWGRIDPTDFNKKLDKLGRRADELLQELVDEIR-- 269
           + +FRE+I++MM    + N GDF+PL   +D  +  K+  ++ +R++  L+ L++E R  
Sbjct: 209 AKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCKRIAKRSNAFLEGLIEEHRRG 268

Query: 270 NQEDDGNTMIHHLLRLQNTDPEYYSDQVIKGLVQVVFLAGVDTAAVTLEWALSELLNNPE 329
           N   DG TMI HLL+L  + PEYYSD +IKGL+Q + LAG DT+AVT+EW +SELLN+PE
Sbjct: 269 NIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGTDTSAVTIEWVMSELLNHPE 328

Query: 330 VLKKARDEIDSVIGQEGLVNEADLSSLNYLQGIITETLRLNPAAPLLVPHCASEDCKIGG 389
           VLKKA++E+D+ IG+  LV+E DLS L YLQ II+ETLRL+P APLL+PH +SEDC IG 
Sbjct: 329 VLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLHPPAPLLLPHYSSEDCTIGE 388

Query: 390 YDVPRDTIVLINAWAIHRDLSLWEDAASFKPERYENAVGVDSCKLIPFGLGRRACPGMGM 449
           ++VP+DTI+L N W IHRD   W DA SFKPER+E    V+  K++ FGLGRRACPG+ +
Sbjct: 389 FNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEKEEEVN--KVMAFGLGRRACPGLSL 448

Query: 450 AQRVLALTLASLIQCFEWQRLSNSLVDMTEGEGLTMPKAQPLIAKCRPRPIMKEM 503
           AQR +  T+  LIQCFEW+R S   +DM EG+G+TMP   PL A C+  PI  ++
Sbjct: 449 AQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGITMPMKIPLRAMCKALPIANDV 499

BLAST of Bhi10G001169 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 1.2e-142
Identity = 258/506 (50.99%), Postives = 356/506 (70.36%), Query Frame = 0

Query: 5   LLTWILLSLVCLLLGYSFLPKPNRLNLPPTPLFCLPFIGHLHLLKHPVHRTFQNLSQKY- 64
           ++ + + SL+ L++ + FL KP + NLPP+P   LP IGHL LLK P+HRT ++ S+   
Sbjct: 8   VVLYSIFSLIFLIISFKFL-KPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLD 67

Query: 65  ----GRVFSLKFGSRLVVIVSSPS-AVQECFTKNDIILANRPYLETGKYLAYNNTTLVVS 124
               G V SL+ GSRLV +VSS   A +ECF KND++LANRP +  GK++ YNNT ++ +
Sbjct: 68  HNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAA 127

Query: 125 PYGEHWRNLRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNNHGLEDEFRVVELEPM 184
           PYG+HWRNLRR+ T+EIFST RLN F  +R  EV+RL+ +L   +     +  VVEL+PM
Sbjct: 128 PYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRL---SRLAGTKKTVVELKPM 187

Query: 185 LLDLTFNIVMRMVGGNKFCEENNNVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWGR 244
           L+DLTFN +MRM+ G ++  E      DE  + + R+++  +     S N  D++P+   
Sbjct: 188 LMDLTFNNIMRMMTGKRYYGEETT---DEEEAKRVRKLVADVGANTSSGNAVDYVPILRL 247

Query: 245 IDPTDFNKKLDKLGRRADELLQELVDEIRNQEDDGNTMIHHLLRLQNTDPEYYSDQVIKG 304
              + +  ++ KLG   D+ LQ L+D+ R Q++ G TMI HLL LQ +D EYY+DQ+IKG
Sbjct: 248 F--SSYENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKG 307

Query: 305 LVQVVFLAGVDTAAVTLEWALSELLNNPEVLKKARDEIDSVIGQEGLVNEADLSSLNYLQ 364
           ++ ++ +AG +T+AVTLEWALS LLN+P+V+ KARDEID+ +G + L+ EADLS L YL+
Sbjct: 308 IILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLK 367

Query: 365 GIITETLRLNPAAPLLVPHCASEDCKIGGYDVPRDTIVLINAWAIHRDLSLWEDAASFKP 424
            I+ ETLRL+PA PLLVPH ASEDCKIG YD+PR T +L+NAWAIHRD + W+D  SFKP
Sbjct: 368 NIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKP 427

Query: 425 ERYENAVGVDSCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSNSLVDMTEG 484
           ER+E     ++ KL+ FGLGRRACPG G+AQR++ L L SLIQCFEW+R+ N  VDM EG
Sbjct: 428 ERFEKE--EEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEG 487

Query: 485 EGLTMPKAQPLIAKCRPRPIMKEMLS 505
            G T+PKA PL A C+ RP + +++S
Sbjct: 488 VGNTVPKAIPLKAICKARPFLHKIIS 502

BLAST of Bhi10G001169 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 1.6e-139
Identity = 253/494 (51.21%), Postives = 342/494 (69.23%), Query Frame = 0

Query: 9   ILLSLVCLLLGYSFLPKPNR--LNLPPTPLFCLPFIGHLHLLKHPVHRTFQNLSQKYGRV 68
           +++SL  L++        +R   NLPP P  CLP IG+LH LK P+H TF  LSQKYG++
Sbjct: 8   LIISLFFLIITLKVFFNTSRKFKNLPPGPQ-CLPIIGNLHQLKQPLHHTFHTLSQKYGQI 67

Query: 69  FSLKFGSRLVVIVSSPSAVQECFTKNDIILANRPYLETGKYLAYNNTTLVVSPYGEHWRN 128
           FSL FGSRLVV+VSS +  QECFTKNDI+LANRP+  TGKY+ YNNTT+  SPYG+HWRN
Sbjct: 68  FSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRN 127

Query: 129 LRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNNHGLEDEFRVVELEPMLLDLTFNI 188
           LRRI ++EI S+ RLNSF  IR  E+ RL++KL   ++   + F  VEL PM  ++TFN 
Sbjct: 128 LRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSY---NGFTEVELRPMFSEMTFNT 187

Query: 189 VMRMVGGNKFCEENNNVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWGRIDPTDFNK 248
           +MRMV G ++   + +V  D   +  FR +I +++  G + N GDF+      D     K
Sbjct: 188 IMRMVSGKRYYGNDCDV-SDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEK 247

Query: 249 KLDKLGRRADELLQELVDEIRNQEDDGNTMIHHLLRLQNTDPEYYSDQVIKGLVQVVFLA 308
           +L K+ +R D  LQ L+DE R  + + NTMI HLL  Q + PEYY+DQ+IKGL+ V+ LA
Sbjct: 248 RLKKISKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLA 307

Query: 309 GVDTAAVTLEWALSELLNNPEVLKKARDEIDSVIGQEGLVNEADLSSLNYLQGIITETLR 368
           G DT++VT+EWA+S LLN+PE++KKA++E+D+ IG +  V+E D+S L YLQ I+ ETLR
Sbjct: 308 GTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLR 367

Query: 369 LNPAAPLLVPHCASEDCKIGGYDVPRDTIVLINAWAIHRDLSLWEDAASFKPERYENAVG 428
           L+ AAPLLVPH +SED  +GGY++P++TI+++NAW IHRD +LW D   FKPER+E    
Sbjct: 368 LHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEKEGE 427

Query: 429 VDSCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSNSLVDMTEGEGLTMPKA 488
           V+  KL+ FGLGRRACPG  ++QR   LTL  LIQCFEW+R+    +DM E +G+T  K 
Sbjct: 428 VN--KLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITAGKK 487

Query: 489 QPLIAKCRPRPIMK 501
             L A C+ R  +K
Sbjct: 488 TSLNAMCKVRHPLK 494

BLAST of Bhi10G001169 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 496.5 bits (1277), Expect = 3.6e-139
Identity = 242/473 (51.16%), Postives = 339/473 (71.67%), Query Frame = 0

Query: 30  NLPPTPLFCLPFIGHLHLLKHPVHRTFQNLSQKYGRVFSLKFGSRLVVIVSSPSAVQECF 89
           NLPP P   LP IG+LH LK P+HRTF+ LS+KYG VFSL FGSRLVV+VSS S  Q+CF
Sbjct: 31  NLPPGPP-SLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLVVVVSSASEFQQCF 90

Query: 90  TKNDIILANRPYLETGKYLAYNNTTLVVSPYGEHWRNLRRISTLEIFSTTRLNSFSRIRE 149
           TKND++LANRP   +GKY+ YN TTL  + YGEHWRNLRRI+ L++ S  R+NSFS IR 
Sbjct: 91  TKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRINSFSGIRR 150

Query: 150 HEVKRLLRKLCGNNHGLEDEFRVVELEPMLLDLTFNIVMRMVGGNKFCEENNNVLEDEGY 209
            E +RL+ +L  ++      F  +EL   L D+TFN +MRM+ G ++  E+ +   D   
Sbjct: 151 DETQRLITRLADDS---STNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCDT-SDLQE 210

Query: 210 SNKFREMITQMMKYGVSTNPGDFIPLWGRIDPTDFNKKLDKLGRRADELLQELVDEIRNQ 269
           +++FR+M++++++   + N  DF+PL   +D  +  K+L  +  + D  L+ L++E R +
Sbjct: 211 ASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIEEHRTK 270

Query: 270 EDDGNTMIHHLLRLQNTDPEYYSDQVIKGLVQVVFLAGVDTAAVTLEWALSELLNNPEVL 329
           ++  NTMI HLL LQ++ PEYY+DQ+IKGL   + LAG D++AVTLEW++S LLN+PEVL
Sbjct: 271 KERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVL 330

Query: 330 KKARDEIDSVIGQEGLVNEADLSSLNYLQGIITETLRLNPAAPLLVPHCASEDCKIGGYD 389
           KK +DE+D+ +GQ+ LV+E+DL  L YL+ +I ETLRL   APLL+PH  S++C IGGY 
Sbjct: 331 KKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTPAPLLLPHSTSDECNIGGYK 390

Query: 390 VPRDTIVLINAWAIHRDLSLWEDAASFKPERYENAVGVDSCKLIPFGLGRRACPGMGMAQ 449
           VP+DTIVLINAWAIHRD  LW +A +FKPER+E    ++  KLI FG+GRRACPG G+A 
Sbjct: 391 VPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKGELE--KLIAFGMGRRACPGEGLAI 450

Query: 450 RVLALTLASLIQCFEWQRLSNSLVDMTEGEGLTMPKAQPLIAKCRPRPIMKEM 503
           R +++TLA LIQCF+W+ ++   +D+ E +G T+ K  PL A C+ RP++ ++
Sbjct: 451 RAISMTLALLIQCFDWKLINGDKIDLAERDGFTLTKLVPLKAMCKSRPVINKV 496

BLAST of Bhi10G001169 vs. NCBI nr
Match: XP_038902744.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 1028.5 bits (2658), Expect = 2.0e-296
Identity = 507/507 (100.00%), Postives = 507/507 (100.00%), Query Frame = 0

Query: 1   MDFDLLTWILLSLVCLLLGYSFLPKPNRLNLPPTPLFCLPFIGHLHLLKHPVHRTFQNLS 60
           MDFDLLTWILLSLVCLLLGYSFLPKPNRLNLPPTPLFCLPFIGHLHLLKHPVHRTFQNLS
Sbjct: 1   MDFDLLTWILLSLVCLLLGYSFLPKPNRLNLPPTPLFCLPFIGHLHLLKHPVHRTFQNLS 60

Query: 61  QKYGRVFSLKFGSRLVVIVSSPSAVQECFTKNDIILANRPYLETGKYLAYNNTTLVVSPY 120
           QKYGRVFSLKFGSRLVVIVSSPSAVQECFTKNDIILANRPYLETGKYLAYNNTTLVVSPY
Sbjct: 61  QKYGRVFSLKFGSRLVVIVSSPSAVQECFTKNDIILANRPYLETGKYLAYNNTTLVVSPY 120

Query: 121 GEHWRNLRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNNHGLEDEFRVVELEPMLL 180
           GEHWRNLRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNNHGLEDEFRVVELEPMLL
Sbjct: 121 GEHWRNLRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNNHGLEDEFRVVELEPMLL 180

Query: 181 DLTFNIVMRMVGGNKFCEENNNVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWGRID 240
           DLTFNIVMRMVGGNKFCEENNNVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWGRID
Sbjct: 181 DLTFNIVMRMVGGNKFCEENNNVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWGRID 240

Query: 241 PTDFNKKLDKLGRRADELLQELVDEIRNQEDDGNTMIHHLLRLQNTDPEYYSDQVIKGLV 300
           PTDFNKKLDKLGRRADELLQELVDEIRNQEDDGNTMIHHLLRLQNTDPEYYSDQVIKGLV
Sbjct: 241 PTDFNKKLDKLGRRADELLQELVDEIRNQEDDGNTMIHHLLRLQNTDPEYYSDQVIKGLV 300

Query: 301 QVVFLAGVDTAAVTLEWALSELLNNPEVLKKARDEIDSVIGQEGLVNEADLSSLNYLQGI 360
           QVVFLAGVDTAAVTLEWALSELLNNPEVLKKARDEIDSVIGQEGLVNEADLSSLNYLQGI
Sbjct: 301 QVVFLAGVDTAAVTLEWALSELLNNPEVLKKARDEIDSVIGQEGLVNEADLSSLNYLQGI 360

Query: 361 ITETLRLNPAAPLLVPHCASEDCKIGGYDVPRDTIVLINAWAIHRDLSLWEDAASFKPER 420
           ITETLRLNPAAPLLVPHCASEDCKIGGYDVPRDTIVLINAWAIHRDLSLWEDAASFKPER
Sbjct: 361 ITETLRLNPAAPLLVPHCASEDCKIGGYDVPRDTIVLINAWAIHRDLSLWEDAASFKPER 420

Query: 421 YENAVGVDSCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSNSLVDMTEGEG 480
           YENAVGVDSCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSNSLVDMTEGEG
Sbjct: 421 YENAVGVDSCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSNSLVDMTEGEG 480

Query: 481 LTMPKAQPLIAKCRPRPIMKEMLSMKQ 508
           LTMPKAQPLIAKCRPRPIMKEMLSMKQ
Sbjct: 481 LTMPKAQPLIAKCRPRPIMKEMLSMKQ 507

BLAST of Bhi10G001169 vs. NCBI nr
Match: KAA0032538.1 (isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 810.8 bits (2093), Expect = 6.5e-231
Identity = 402/508 (79.13%), Postives = 454/508 (89.37%), Query Frame = 0

Query: 1   MDFDLLTWILLSLVCLLLGYSFLPKPNRLNLPPTPLFCLPFIGHLHLLKHPVHRTFQNLS 60
           MDFD L + LLSL+ LLLGYSFL KP+RLNLPPTPLF LPFIGHLHLLKHPVH+TFQ LS
Sbjct: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60

Query: 61  QKYGRVFSLKFGSRLVVIVSSPSAVQECFTKNDIILANRPYLETGKYLAYNNTTLVVSPY 120
           QKYG VFSLKFGSRLVV+VSSPSA+QECFTKNDIILANRP L +GKYLAYNNTT+VVS Y
Sbjct: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120

Query: 121 GEHWRNLRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNNHGLEDEFRVVELEPMLL 180
           GEHWRNLRRISTLEIFSTTRLNSFSR RE EVKRLLRKL G N+ LEDEFRVVELEPMLL
Sbjct: 121 GEHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWG-NYKLEDEFRVVELEPMLL 180

Query: 181 DLTFNIVMRMVGGNKFCEE-NNNVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWGRI 240
           DLTFNIVMRMVGG KFCEE NNNVLEDEGY  +F+E++TQ+M +G STNPGDFIPL   I
Sbjct: 181 DLTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWI 240

Query: 241 DPTDFNKKLDKLGRRADELLQELVDEIRNQEDDGNTMIHHLLRLQNTDPEYYSDQVIKGL 300
           DP+ + K++ K+GR+ DE+LQ LVDEIRN+ED+GNTMI HLLRLQ TDPEYYSDQ+IKGL
Sbjct: 241 DPSGYTKRIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGL 300

Query: 301 VQVVFLAGVDTAAVTLEWALSELLNNPEVLKKARDEIDSVIGQEGLVNEADLSSLNYLQG 360
           VQ + LAG+DT+AVTL+WALS LLNNP VL+KA+ EIDS IGQE +VNEADLSSL+YLQG
Sbjct: 301 VQDILLAGIDTSAVTLQWALSHLLNNPIVLEKAKAEIDSYIGQERMVNEADLSSLSYLQG 360

Query: 361 IITETLRLNPAAPLLVPHCASEDCKIGGYDVPRDTIVLINAWAIHRDLSLWEDAASFKPE 420
           II+ETLRL+PA PLLVPHC+SEDCKIGGYDVPR+TIVLINAWAIHRD +LWEDA+SFKPE
Sbjct: 361 IISETLRLSPAGPLLVPHCSSEDCKIGGYDVPRNTIVLINAWAIHRDPNLWEDASSFKPE 420

Query: 421 RYENAVGVD-SCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSNSLVDMTEG 480
           R+ NA G + S KL+PFG+GRRACPGM MAQRV+ LTLASL+QCFEW+R+S+ LVDM EG
Sbjct: 421 RHVNAAGFENSYKLLPFGMGRRACPGMAMAQRVIGLTLASLVQCFEWKRMSDLLVDMREG 480

Query: 481 EGLTMPKAQPLIAKCRPRPIMKEMLSMK 507
           EGLTMPK +PL+AKCRPR IM+ +LS K
Sbjct: 481 EGLTMPKVEPLVAKCRPRFIMEAVLSEK 507

BLAST of Bhi10G001169 vs. NCBI nr
Match: XP_038902403.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 810.8 bits (2093), Expect = 6.5e-231
Identity = 399/507 (78.70%), Postives = 444/507 (87.57%), Query Frame = 0

Query: 1   MDFDLLTWILLSLVCLLLGYSFLPKPNRLNLPPTPLFCLPFIGHLHLLKHPVHRTFQNLS 60
           MDFD+L  ILLSLVCLLLGY+ L KPNRLNLPPTPLFCLPFIGHLHLLKHPVH+TFQNLS
Sbjct: 1   MDFDILFCILLSLVCLLLGYTSLLKPNRLNLPPTPLFCLPFIGHLHLLKHPVHQTFQNLS 60

Query: 61  QKYGRVFSLKFGSRLVVIVSSPSAVQECFTKNDIILANRPYLETGKYLAYNNTTLVVSPY 120
           QKYG VFSLKFGSRL+V+VSSPS VQECFTKNDIILANRP L TGKYLAYNN T+ VS Y
Sbjct: 61  QKYGHVFSLKFGSRLIVLVSSPSVVQECFTKNDIILANRPSLNTGKYLAYNNITMAVSSY 120

Query: 121 GEHWRNLRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNNHGLEDEFRVVELEPMLL 180
           GEHWRNLRRISTLEIFS TR+NSF R RE EVKRLL KLCGN + LED+FR V+L+PMLL
Sbjct: 121 GEHWRNLRRISTLEIFSMTRVNSFLRTREDEVKRLLCKLCGNCYTLEDKFRAVDLKPMLL 180

Query: 181 DLTFNIVMRMVGGNKFCEE--NNNVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWGR 240
           DLTFNIVMRMVGG KFCEE  NNNVLEDEGYS +FRE++TQ+M +  STNP DFIPLW  
Sbjct: 181 DLTFNIVMRMVGGKKFCEENNNNNVLEDEGYSKRFREVVTQIMAHSGSTNPEDFIPLWNW 240

Query: 241 IDPTDFNKKLDKLGRRADELLQELVDEIRNQEDDGNTMIHHLLRLQNTDPEYYSDQVIKG 300
           IDPT FNK++ KLGRRADE+LQ LVDEIRNQED+GN MIHHLLRLQNTD EYYSDQVIKG
Sbjct: 241 IDPTGFNKRIMKLGRRADEILQGLVDEIRNQEDEGNNMIHHLLRLQNTDLEYYSDQVIKG 300

Query: 301 LVQVVFLAGVDTAAVTLEWALSELLNNPEVLKKARDEIDSVIGQEGLVNEADLSSLNYLQ 360
           L+  + +AG+DT AVTLEWALS LLNNP VL++A+ EID  IGQE +VNEADLSSL+YLQ
Sbjct: 301 LIHDILIAGIDTTAVTLEWALSHLLNNPIVLERAKAEIDCSIGQERMVNEADLSSLSYLQ 360

Query: 361 GIITETLRLNPAAPLLVPHCASEDCKIGGYDVPRDTIVLINAWAIHRDLSLWEDAASFKP 420
           GII+ETLRL+P  PLLVPHCASEDCKIGGYD+PR TIVLIN WAIHRD +LWEDA SFKP
Sbjct: 361 GIISETLRLSPPGPLLVPHCASEDCKIGGYDIPRKTIVLINVWAIHRDPNLWEDATSFKP 420

Query: 421 ERYENAVGVD-SCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSNSLVDMTE 480
           ER+ N   V+ S KL+PFGLGRRACPGM MAQR++ LTLASLIQCFEW+R S SLVDM+E
Sbjct: 421 ERHANVAEVEYSYKLLPFGLGRRACPGMAMAQRIIGLTLASLIQCFEWKRTSKSLVDMSE 480

Query: 481 GEGLTMPKAQPLIAKCRPRPIMKEMLS 505
           G+GLTMPK +P++AKCRPR IMK +LS
Sbjct: 481 GKGLTMPKVEPIMAKCRPRLIMKAVLS 507

BLAST of Bhi10G001169 vs. NCBI nr
Match: XP_004151924.2 (cytochrome P450 81Q32 [Cucumis sativus] >KGN63284.1 hypothetical protein Csa_022055 [Cucumis sativus])

HSP 1 Score: 793.1 bits (2047), Expect = 1.4e-225
Identity = 393/510 (77.06%), Postives = 444/510 (87.06%), Query Frame = 0

Query: 1   MDFDLLTWILLSLVCLLLGYSFLPKPNRLNLPPTPLFCLPFIGHLHLLKHPVHRTFQNLS 60
           MDFD L + LLSL+ LLLGYSFL KP+RLNLPPTPLF LPFIGHLHLLKHPVH+T Q LS
Sbjct: 1   MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60

Query: 61  QKYGRVFSLKFGSRLVVIVSSPSAVQECFTKNDIILANRPYLETGKYLAYNNTTLVVSPY 120
           QKYG VFSLKFGSRLV++VSSPSAVQECFTKNDIILANRP L +GKYL+YNNTT+ VSPY
Sbjct: 61  QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120

Query: 121 GEHWRNLRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNNHGLEDEFRVVELEPMLL 180
           GEHWRNLRRI TLEIFSTTRLNSFSRIRE EVKRLL KLCG N+  EDEFRVVELE MLL
Sbjct: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCG-NYKFEDEFRVVELESMLL 180

Query: 181 DLTFNIVMRMVGGNKFCEENN---NVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWG 240
           DLT NIVMRMVGG KFC+ENN   NVLEDEGY  + +E++TQ+M +  STNPGDFIPLW 
Sbjct: 181 DLTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWN 240

Query: 241 RIDPTDFNKKLDKLGRRADELLQELVDEIRNQEDDGNTMIHHLLRLQNTDPEYYSDQVIK 300
            IDP+ +NK++ K+GRR DE+LQ LVDEIRN+ED+GNTMI HLLRLQ TDP+YYSD +IK
Sbjct: 241 WIDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIK 300

Query: 301 GLVQVVFLAGVDTAAVTLEWALSELLNNPEVLKKARDEIDSVIGQEGLVNEADLSSLNYL 360
           GL+Q + +AG+DT+AVTL+WALS LLNNP VL KA+ EIDS IGQE +VNE DLSSL+YL
Sbjct: 301 GLIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYL 360

Query: 361 QGIITETLRLNPAAPLLVPHCASEDCKIGGYDVPRDTIVLINAWAIHRDLSLWEDAASFK 420
           QGII+ETLRL+P  PLLVPHCASEDCKIGGYDVPR+TIVLINAWAIHRD ++WEDA SFK
Sbjct: 361 QGIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFK 420

Query: 421 PERYENAVGVD-SCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSNSLVDMT 480
           PER+ NAVG + S KL+PFGLGRRACPGM MAQRV+ LTLASLIQCFEW+++SN LVDM 
Sbjct: 421 PERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMR 480

Query: 481 EGEGLTMPKAQPLIAKCRPRPIMKEMLSMK 507
           EGEGLTMPK + L+AKCRPR IMK +L  K
Sbjct: 481 EGEGLTMPKVESLVAKCRPRFIMKVVLGEK 509

BLAST of Bhi10G001169 vs. NCBI nr
Match: XP_038902241.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 782.3 bits (2019), Expect = 2.5e-222
Identity = 395/506 (78.06%), Postives = 438/506 (86.56%), Query Frame = 0

Query: 1   MDFDLLTWILLSLVCLLLGYSFLPKPNRLNLPPTPLFCLPFIGHLHLLKHPVHRTFQNLS 60
           MDFDLL  ILLSLV LLLGYSFL KP+ LNLPPTPLFCLPFIGHLHLLKHPVH+ FQNLS
Sbjct: 1   MDFDLLLCILLSLVYLLLGYSFLLKPHGLNLPPTPLFCLPFIGHLHLLKHPVHKIFQNLS 60

Query: 61  QKYGRVFSLKFGSRLVVIVSSPSAVQECFTKNDIILANRPYLETGKYLAYNNTTLVVSPY 120
           QKYG VFSLKFGS LVV+VSSPSAVQECFTKNDIILANRP L  GKYLAYNNT++ +SPY
Sbjct: 61  QKYGHVFSLKFGSHLVVLVSSPSAVQECFTKNDIILANRPSLNPGKYLAYNNTSMALSPY 120

Query: 121 GEHWRNLRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNNHGLEDEFRVVELEPMLL 180
           GEHWRNLRRISTLEIFS TR N F + RE EVKRLL KLCGN+  LEDEFRVVELEPMLL
Sbjct: 121 GEHWRNLRRISTLEIFSATRFNLFLKSREDEVKRLLYKLCGND-TLEDEFRVVELEPMLL 180

Query: 181 DLTFNIVMRMVGGNKFCEENN-NVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWGRI 240
           DLTFNIVMRMVGG KFCEENN NV EDEGYS +FRE++TQ+M +G STNPGDFIPLW  I
Sbjct: 181 DLTFNIVMRMVGGKKFCEENNKNVSEDEGYSKRFRELVTQIMAHGGSTNPGDFIPLWNWI 240

Query: 241 DPTDFNKKLDKLGRRADELLQELVDEIRNQEDDGNTMIHHLLRLQNTDPEYYSDQVIKGL 300
           DPT +NK+L KLGRR+DE+LQ L+DEIRNQED+G  MI HLLRLQNTDPEYYSDQVIKGL
Sbjct: 241 DPTGYNKRLMKLGRRSDEVLQGLIDEIRNQEDEGINMIQHLLRLQNTDPEYYSDQVIKGL 300

Query: 301 VQVVFLAGVDTAAVTLEWALSELLNNPEVLKKARDEIDSVIGQEGLVNEADLSSLNYLQG 360
           VQ + L G+DT+AVTLEWALS LLNNP VL++A+ EID  IGQE +VNEADLSSL+YLQG
Sbjct: 301 VQDILLGGIDTSAVTLEWALSHLLNNPIVLERAKAEIDCFIGQERMVNEADLSSLSYLQG 360

Query: 361 IITETLRLNPAAPLLVPHCASEDCKIGGYDVPRDTIVLINAWAIHRDLSLWEDAASFKPE 420
           II+ETLRL+P  PLL PH AS+DC IGGYDVPR+T+VL N WAIHRD +LWEDA SFKPE
Sbjct: 361 IISETLRLSPPGPLL-PHYASKDCTIGGYDVPRNTMVLFNIWAIHRDPNLWEDATSFKPE 420

Query: 421 RYENAVGVD-SCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSNSLVDMTEG 480
           R+ N   V+ S KL+PFGLGRRACPGM +AQRV+ LTLASLIQCFEW+R S SLVDM+EG
Sbjct: 421 RHANVAEVEYSYKLLPFGLGRRACPGMAIAQRVIGLTLASLIQCFEWKRTSKSLVDMSEG 480

Query: 481 EGLTMPKAQPLIAKCRPRPIMKEMLS 505
           +GLTMPKA+PLI K RP  IMK +LS
Sbjct: 481 KGLTMPKAKPLIVKYRPCLIMKAILS 504

BLAST of Bhi10G001169 vs. ExPASy TrEMBL
Match: A0A5A7SPU0 (Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold465G00500 PE=3 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 3.1e-231
Identity = 402/508 (79.13%), Postives = 454/508 (89.37%), Query Frame = 0

Query: 1   MDFDLLTWILLSLVCLLLGYSFLPKPNRLNLPPTPLFCLPFIGHLHLLKHPVHRTFQNLS 60
           MDFD L + LLSL+ LLLGYSFL KP+RLNLPPTPLF LPFIGHLHLLKHPVH+TFQ LS
Sbjct: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60

Query: 61  QKYGRVFSLKFGSRLVVIVSSPSAVQECFTKNDIILANRPYLETGKYLAYNNTTLVVSPY 120
           QKYG VFSLKFGSRLVV+VSSPSA+QECFTKNDIILANRP L +GKYLAYNNTT+VVS Y
Sbjct: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120

Query: 121 GEHWRNLRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNNHGLEDEFRVVELEPMLL 180
           GEHWRNLRRISTLEIFSTTRLNSFSR RE EVKRLLRKL G N+ LEDEFRVVELEPMLL
Sbjct: 121 GEHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWG-NYKLEDEFRVVELEPMLL 180

Query: 181 DLTFNIVMRMVGGNKFCEE-NNNVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWGRI 240
           DLTFNIVMRMVGG KFCEE NNNVLEDEGY  +F+E++TQ+M +G STNPGDFIPL   I
Sbjct: 181 DLTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWI 240

Query: 241 DPTDFNKKLDKLGRRADELLQELVDEIRNQEDDGNTMIHHLLRLQNTDPEYYSDQVIKGL 300
           DP+ + K++ K+GR+ DE+LQ LVDEIRN+ED+GNTMI HLLRLQ TDPEYYSDQ+IKGL
Sbjct: 241 DPSGYTKRIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGL 300

Query: 301 VQVVFLAGVDTAAVTLEWALSELLNNPEVLKKARDEIDSVIGQEGLVNEADLSSLNYLQG 360
           VQ + LAG+DT+AVTL+WALS LLNNP VL+KA+ EIDS IGQE +VNEADLSSL+YLQG
Sbjct: 301 VQDILLAGIDTSAVTLQWALSHLLNNPIVLEKAKAEIDSYIGQERMVNEADLSSLSYLQG 360

Query: 361 IITETLRLNPAAPLLVPHCASEDCKIGGYDVPRDTIVLINAWAIHRDLSLWEDAASFKPE 420
           II+ETLRL+PA PLLVPHC+SEDCKIGGYDVPR+TIVLINAWAIHRD +LWEDA+SFKPE
Sbjct: 361 IISETLRLSPAGPLLVPHCSSEDCKIGGYDVPRNTIVLINAWAIHRDPNLWEDASSFKPE 420

Query: 421 RYENAVGVD-SCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSNSLVDMTEG 480
           R+ NA G + S KL+PFG+GRRACPGM MAQRV+ LTLASL+QCFEW+R+S+ LVDM EG
Sbjct: 421 RHVNAAGFENSYKLLPFGMGRRACPGMAMAQRVIGLTLASLVQCFEWKRMSDLLVDMREG 480

Query: 481 EGLTMPKAQPLIAKCRPRPIMKEMLSMK 507
           EGLTMPK +PL+AKCRPR IM+ +LS K
Sbjct: 481 EGLTMPKVEPLVAKCRPRFIMEAVLSEK 507

BLAST of Bhi10G001169 vs. ExPASy TrEMBL
Match: A0A0A0LQI5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 6.8e-226
Identity = 393/510 (77.06%), Postives = 444/510 (87.06%), Query Frame = 0

Query: 1   MDFDLLTWILLSLVCLLLGYSFLPKPNRLNLPPTPLFCLPFIGHLHLLKHPVHRTFQNLS 60
           MDFD L + LLSL+ LLLGYSFL KP+RLNLPPTPLF LPFIGHLHLLKHPVH+T Q LS
Sbjct: 1   MDFDHLLYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLS 60

Query: 61  QKYGRVFSLKFGSRLVVIVSSPSAVQECFTKNDIILANRPYLETGKYLAYNNTTLVVSPY 120
           QKYG VFSLKFGSRLV++VSSPSAVQECFTKNDIILANRP L +GKYL+YNNTT+ VSPY
Sbjct: 61  QKYGHVFSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPY 120

Query: 121 GEHWRNLRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNNHGLEDEFRVVELEPMLL 180
           GEHWRNLRRI TLEIFSTTRLNSFSRIRE EVKRLL KLCG N+  EDEFRVVELE MLL
Sbjct: 121 GEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCG-NYKFEDEFRVVELESMLL 180

Query: 181 DLTFNIVMRMVGGNKFCEENN---NVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWG 240
           DLT NIVMRMVGG KFC+ENN   NVLEDEGY  + +E++TQ+M +  STNPGDFIPLW 
Sbjct: 181 DLTSNIVMRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWN 240

Query: 241 RIDPTDFNKKLDKLGRRADELLQELVDEIRNQEDDGNTMIHHLLRLQNTDPEYYSDQVIK 300
            IDP+ +NK++ K+GRR DE+LQ LVDEIRN+ED+GNTMI HLLRLQ TDP+YYSD +IK
Sbjct: 241 WIDPSGYNKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIK 300

Query: 301 GLVQVVFLAGVDTAAVTLEWALSELLNNPEVLKKARDEIDSVIGQEGLVNEADLSSLNYL 360
           GL+Q + +AG+DT+AVTL+WALS LLNNP VL KA+ EIDS IGQE +VNE DLSSL+YL
Sbjct: 301 GLIQDILIAGIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYL 360

Query: 361 QGIITETLRLNPAAPLLVPHCASEDCKIGGYDVPRDTIVLINAWAIHRDLSLWEDAASFK 420
           QGII+ETLRL+P  PLLVPHCASEDCKIGGYDVPR+TIVLINAWAIHRD ++WEDA SFK
Sbjct: 361 QGIISETLRLSPPGPLLVPHCASEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFK 420

Query: 421 PERYENAVGVD-SCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSNSLVDMT 480
           PER+ NAVG + S KL+PFGLGRRACPGM MAQRV+ LTLASLIQCFEW+++SN LVDM 
Sbjct: 421 PERHVNAVGFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMR 480

Query: 481 EGEGLTMPKAQPLIAKCRPRPIMKEMLSMK 507
           EGEGLTMPK + L+AKCRPR IMK +L  K
Sbjct: 481 EGEGLTMPKVESLVAKCRPRFIMKVVLGEK 509

BLAST of Bhi10G001169 vs. ExPASy TrEMBL
Match: A0A1S3C1S2 (LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103495921 PE=3 SV=1)

HSP 1 Score: 762.3 bits (1967), Expect = 1.3e-216
Identity = 379/494 (76.72%), Postives = 433/494 (87.65%), Query Frame = 0

Query: 1   MDFDLLTWILLSLVCLLLGYSFLPKPNRLNLPPTPLFCLPFIGHLHLLKHPVHRTFQNLS 60
           MDFD L + LLSL+ LLLGYSFL KP+RLNLPPTPLF LPFIGHLHLLKHPVH+TFQ LS
Sbjct: 1   MDFDHLLYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLS 60

Query: 61  QKYGRVFSLKFGSRLVVIVSSPSAVQECFTKNDIILANRPYLETGKYLAYNNTTLVVSPY 120
           QKYG VFSLKFGSRLVV+VSSPSA+QECFTKNDIILANRP L +GKYLAYNNTT+VVS Y
Sbjct: 61  QKYGHVFSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSY 120

Query: 121 GEHWRNLRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNNHGLEDEFRVVELEPMLL 180
           G+HWRNLRRISTLEIFSTTRLNSFSR RE EVKRLLRKL G N+ LEDEFRVVELEPMLL
Sbjct: 121 GKHWRNLRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWG-NYKLEDEFRVVELEPMLL 180

Query: 181 DLTFNIVMRMVGGNKFCEE-NNNVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWGRI 240
           DLTFNIVMRMVGG KFCEE NNNVLEDEGY  +F+E++TQ+M +G STNPGDFIPL   I
Sbjct: 181 DLTFNIVMRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWI 240

Query: 241 DPTDFNKKLDKLGRRADELLQELVDEIRNQEDDGNTMIHHLLRLQNTDPEYYSDQVIKGL 300
           DP+ + K + K+GR+ DE+LQ LVDEIRN+ED+GNTMI HLLRLQ TDPEYYSDQ+IKGL
Sbjct: 241 DPSGYTKNIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGL 300

Query: 301 VQVVFLAGVDTAAVTLEWALSELLNNPEVLKKARDEIDSVIGQEGLVNEADLSSLNYLQG 360
           VQ + LAG+DT+ ++LEWALS+LLNNPEVLKKA++EID +IG + LV+E DL SL+YLQG
Sbjct: 301 VQDIILAGIDTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQG 360

Query: 361 IITETLRLNPAAPLLVPHCA-SEDCKIGGYDVPRDTIVLINAWAIHRDLSLWEDAASFKP 420
           II+ETLRL+P APLLVPHCA  E+CKI  Y++PRDTIVLINAWAIHRD  LWEDA  FKP
Sbjct: 361 IISETLRLHPPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKP 420

Query: 421 ERYENAVGVDSCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSNSLVDMTEG 480
           ER+ + +  +  KLIPFG+GRRACPG GMAQRV+ L LASLIQ FEW+R+ +SL+DM+EG
Sbjct: 421 ERH-SVIESELYKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEG 480

Query: 481 EGLTMPKAQPLIAK 493
           +GLTMPKAQPLIAK
Sbjct: 481 KGLTMPKAQPLIAK 492

BLAST of Bhi10G001169 vs. ExPASy TrEMBL
Match: A0A6J1G5P7 (cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1)

HSP 1 Score: 732.3 bits (1889), Expect = 1.4e-207
Identity = 359/502 (71.51%), Postives = 421/502 (83.86%), Query Frame = 0

Query: 5   LLTWILLSLVCLLLGYSFLPKPNRLNLPPTPLFCLPFIGHLHLLKHPVHRTFQNLSQKYG 64
           LL  I L L+ LL  ++FL  P+RLNLPPTPLF LP IGH HLLKHP+HR+   LSQK+G
Sbjct: 4   LLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHALSQKHG 63

Query: 65  RVFSLKFGSRLVVIVSSPSAVQECFTKNDIILANRPYLETGKYLAYNNTTLVVSPYGEHW 124
            V SL+FGSRLVV++SSPSAVQECFTKNDIILANRP L+TGKYLAYN+TTL VSPYGEHW
Sbjct: 64  HVISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHW 123

Query: 125 RNLRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNNHGLEDEFRVVELEPMLLDLTF 184
           RNLRRI +LEIFST RLN F  IRE+EVKRLLRKLCG+     +EFR VELEPML DLTF
Sbjct: 124 RNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGD---WSEEFREVELEPMLSDLTF 183

Query: 185 NIVMRMVGGNKFCEENNNVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWGRIDPTDF 244
           N+VM MVGG +F         DEGYS KFR+++TQ+M +G S+NPGDFIPLW  +DPT F
Sbjct: 184 NVVMGMVGGRRF--------YDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGF 243

Query: 245 NKKLDKLGRRADELLQELVDEIRNQEDDGNTMIHHLLRLQNTDPEYYSDQVIKGLVQVVF 304
            K++  L RR DELLQEL+DE R+ +D GNTMIHHLL LQ  +PEYYSD +IKGL+QV+F
Sbjct: 244 KKRIMNLARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIF 303

Query: 305 LAGVDTAAVTLEWALSELLNNPEVLKKARDEIDSVIGQEGLVNEADLSSLNYLQGIITET 364
           LAG+DT+AVTLEWALS LLNNP+VLKKAR EID +IG+E LVNE+DL +L YLQGII+ET
Sbjct: 304 LAGIDTSAVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLKYLQGIISET 363

Query: 365 LRLNPAAPLLVPHCASEDCKIGGYDVPRDTIVLINAWAIHRDLSLWEDAASFKPERYENA 424
           LRLNPAAPLLVPHCA +DCKI GYDVPRDTIVLIN WAIHRD +LWED  +FKPER+ + 
Sbjct: 364 LRLNPAAPLLVPHCAFQDCKIEGYDVPRDTIVLINTWAIHRDPNLWEDPTAFKPERHGDT 423

Query: 425 VGVDSCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSNSLVDMTEGEGLTMP 484
            G DS KL+PFGLGRRACPG+GMAQRV+ L LASLIQ FEW+R+S+SLVDM+EG+GLTMP
Sbjct: 424 SGADSYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMP 483

Query: 485 KAQPLIAKCRPRPIMKEMLSMK 507
           KAQPL+AKC+PRPIMK +++++
Sbjct: 484 KAQPLVAKCKPRPIMKAIMNVE 494

BLAST of Bhi10G001169 vs. ExPASy TrEMBL
Match: A0A6J1D5S6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE=3 SV=1)

HSP 1 Score: 731.9 bits (1888), Expect = 1.9e-207
Identity = 361/502 (71.91%), Postives = 423/502 (84.26%), Query Frame = 0

Query: 5   LLTWILLSLVCLLLGYSFLPKPNRLNLPPTPLFCLPFIGHLHLLKHPVHRTFQNLSQKYG 64
           LL  I LSL  L + + FL +P R NLPP+P F LP IGHLHL+KHPVHRT   LS  YG
Sbjct: 84  LLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSHNYG 143

Query: 65  RVFSLKFGSRLVVIVSSPSAVQECFTKNDIILANRPYLETGKYLAYNNTTLVVSPYGEHW 124
            VFSL++GSRLVV+VSSPSAVQECFTKNDI+LANRP L++GK+LAYN+TTL VSPYGEHW
Sbjct: 144 HVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYGEHW 203

Query: 125 RNLRRISTLEIFSTTRLNSFSRIREHEVKRLLRKLCGNNHGLEDEFRVVELEPMLLDLTF 184
           RNLRRISTLEIFST+RLN F  IRE EVK LLRKL G++     EFR VEL+ MLLD TF
Sbjct: 204 RNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDS---LQEFRAVELDSMLLDHTF 263

Query: 185 NIVMRMVGGNKFCEENNNVLEDEGYSNKFREMITQMMKYGVSTNPGDFIPLWGRIDPTDF 244
           NIV+RM+ G K+  E+   L DEG S KFREM+ +++  G + N GDF+PLW  IDPT +
Sbjct: 264 NIVVRMIAGKKYYGED---LRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGY 323

Query: 245 NKKLDKLGRRADELLQELVDEIRNQEDDGNTMIHHLLRLQNTDPEYYSDQVIKGLVQVVF 304
            K++ K+G R+DELLQELVDEIRNQ+D GNTMI HLL LQNT PEY+SDQ+IKGL Q++ 
Sbjct: 324 EKRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILL 383

Query: 305 LAGVDTAAVTLEWALSELLNNPEVLKKARDEIDSVIGQEGLVNEADLSSLNYLQGIITET 364
           LAG+DTAA+TLEWALS+LLNNPEVLKKAR EID  IG++ LV+E DLSSL+YLQG+ITET
Sbjct: 384 LAGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITET 443

Query: 365 LRLNPAAPLLVPHCASEDCKIGGYDVPRDTIVLINAWAIHRDLSLWEDAASFKPERYENA 424
           LRLNPAAPLLVPHCASEDCKI GYD+PRDTI+LINAWAIHRD ++WEDA SFKPER+ N 
Sbjct: 444 LRLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANT 503

Query: 425 V-GVDSCKLIPFGLGRRACPGMGMAQRVLALTLASLIQCFEWQRLSNSLVDMTEGEGLTM 484
           + GVDS KL+PFGLGRRACPGMGMAQRV+ LTLASLIQCFEW+R+++SLVDMTEGEGLTM
Sbjct: 504 MGGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTM 563

Query: 485 PKAQPLIAKCRPRPIMKEMLSM 506
           PKAQPL+AKC+PRPI+K +L +
Sbjct: 564 PKAQPLVAKCKPRPILKAILGV 579