Bhi10G000942 (gene) Wax gourd (B227) v1

Overview
NameBhi10G000942
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionCalcium-binding EF-hand family protein
Locationchr10: 26731811 .. 26732921 (-)
RNA-Seq ExpressionBhi10G000942
SyntenyBhi10G000942
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATCACGTGAGGAAGCTTTTATTGTAAAGAATGGTCTTAAAAGATAGAGATATGAAAATATTGTTTTAACCCAAAAAATAGTAAGGAACTCATTCATGCGGCATGGGTTAAGCAAAGTCAAAAAATGTTTATAGTAAGAAAATGACGAGAATAGCCTTATCAAAGGTTCTAGGAAGGCGAGACGCAATTGGCAATAGAATAGCAGTCCAGTTGAGGCAACGTGAGATGCTGCGCTGTCATATGCTGAAGAGATCGCATCTCTCCAATTTCTCCGTCTTTTCTTCCCTTTCCAATCTTTTCACCTTATAAATTCCGTTTCCTCTCCACCCAAAATTTACACAATAATTCAACAAAATTCTCGTATCAATTGTTTAAAAATAATTAAATGGCGAGAAAGGTTGGTTCTAATTTCCAGGACCTGCTTCCGGTAATGGCAGAGAAACTGGGGGGAGAAGGGCTGATAAAGGAGCTGTGTAATGGGTTTGAAGTGCTGATGGACAAAGAGAAAGGGTTGATCACGTTGGAGAGCCTTAGAACAAACTCTTGGCTGTTGGGTGTGCGAGACATGGCCGAGGAGGAGCTTCTTAGTATGATGAGGGAAGGCGATTTGGATGGCGATGGTGCTTTGAATCAAATGGAGTTTTGTGTTTTGATGTTCAGATTAAGCCCTGACTTGATGCAACACTCTTTGAATTGGCTTCAATACGCTTTGGAAAGGGAGTTTAATTGAAACCATATTCTTCATTTTTCTTTTCTTTTCTTTTCTTTTTTTTTTTCCTCTTTTGAGGGGGATTGGAGTTGAGCTAGCTGTTCGATGAAATGACGTCGTTTCATTTTGAAGTTACGAATATGGGAACATGCTTCTACTTTTAATTCCCCTTTATTTCAAGTGTTGCGATGCTATTTTGTGTTCATAACTCCAAATTAGTAAATAATAAAGGAATCGCAAAGTTGTTATTATCATCATCATCATCATTTTTTTTTTCTTGTGCAAGGAACCTTTTCTTTTCTTAGTCCCAAAGTTCGTCAACATGTCAAAATAAGTTATAAAAATTAAGTTCATATCAATACAATATAAAATTTAACAATATAGTAAAATCGGTCGGCTCAG

mRNA sequence

TATCACGTGAGGAAGCTTTTATTGTAAAGAATGGTCTTAAAAGATAGAGATATGAAAATATTGTTTTAACCCAAAAAATAGTAAGGAACTCATTCATGCGGCATGGGTTAAGCAAAGTCAAAAAATGTTTATAGTAAGAAAATGACGAGAATAGCCTTATCAAAGGTTCTAGGAAGGCGAGACGCAATTGGCAATAGAATAGCAGTCCAGTTGAGGCAACGTGAGATGCTGCGCTGTCATATGCTGAAGAGATCGCATCTCTCCAATTTCTCCGTCTTTTCTTCCCTTTCCAATCTTTTCACCTTATAAATTCCGTTTCCTCTCCACCCAAAATTTACACAATAATTCAACAAAATTCTCGTATCAATTGTTTAAAAATAATTAAATGGCGAGAAAGGTTGGTTCTAATTTCCAGGACCTGCTTCCGGTAATGGCAGAGAAACTGGGGGGAGAAGGGCTGATAAAGGAGCTGTGTAATGGGTTTGAAGTGCTGATGGACAAAGAGAAAGGGTTGATCACGTTGGAGAGCCTTAGAACAAACTCTTGGCTGTTGGGTGTGCGAGACATGGCCGAGGAGGAGCTTCTTAGTATGATGAGGGAAGGCGATTTGGATGGCGATGGTGCTTTGAATCAAATGGAGTTTTGTGTTTTGATGTTCAGATTAAGCCCTGACTTGATGCAACACTCTTTGAATTGGCTTCAATACGCTTTGGAAAGGGAGTTTAATTGAAACCATATTCTTCATTTTTCTTTTCTTTTCTTTTCTTTTTTTTTTTCCTCTTTTGAGGGGGATTGGAGTTGAGCTAGCTGTTCGATGAAATGACGTCGTTTCATTTTGAAGTTACGAATATGGGAACATGCTTCTACTTTTAATTCCCCTTTATTTCAAGTGTTGCGATGCTATTTTGTGTTCATAACTCCAAATTAGTAAATAATAAAGGAATCGCAAAGTTGTTATTATCATCATCATCATCATTTTTTTTTTCTTGTGCAAGGAACCTTTTCTTTTCTTAGTCCCAAAGTTCGTCAACATGTCAAAATAAGTTATAAAAATTAAGTTCATATCAATACAATATAAAATTTAACAATATAGTAAAATCGGTCGGCTCAG

Coding sequence (CDS)

ATGGCGAGAAAGGTTGGTTCTAATTTCCAGGACCTGCTTCCGGTAATGGCAGAGAAACTGGGGGGAGAAGGGCTGATAAAGGAGCTGTGTAATGGGTTTGAAGTGCTGATGGACAAAGAGAAAGGGTTGATCACGTTGGAGAGCCTTAGAACAAACTCTTGGCTGTTGGGTGTGCGAGACATGGCCGAGGAGGAGCTTCTTAGTATGATGAGGGAAGGCGATTTGGATGGCGATGGTGCTTTGAATCAAATGGAGTTTTGTGTTTTGATGTTCAGATTAAGCCCTGACTTGATGCAACACTCTTTGAATTGGCTTCAATACGCTTTGGAAAGGGAGTTTAATTGA

Protein sequence

MARKVGSNFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTNSWLLGVRDMAEEELLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLNWLQYALEREFN
Homology
BLAST of Bhi10G000942 vs. TAIR 10
Match: AT4G27280.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 136.0 bits (341), Expect = 1.9e-32
Identity = 68/107 (63.55%), Postives = 83/107 (77.57%), Query Frame = 0

Query: 8   NFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTN-SWLLGVRDMAEEEL 67
           NF D LP MA  LGGEGLI ELCNGFE+LMD+EKG+IT ESLR N + +LG+ D+ +E++
Sbjct: 18  NFHDFLPTMAGNLGGEGLIGELCNGFELLMDREKGVITFESLRRNAAAVLGLGDLTDEDV 77

Query: 68  LSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLNWLQYALEREF 114
             M++EGD D DGALNQMEFCVLMFRLSPDLM+ S   +   +E EF
Sbjct: 78  RCMIKEGDFDCDGALNQMEFCVLMFRLSPDLMEASRCLVTEVIEEEF 124

BLAST of Bhi10G000942 vs. TAIR 10
Match: AT5G54490.1 (pinoid-binding protein 1 )

HSP 1 Score: 131.3 bits (329), Expect = 4.8e-31
Identity = 65/106 (61.32%), Postives = 82/106 (77.36%), Query Frame = 0

Query: 9   FQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTN-SWLLGVRDMAEEELL 68
           FQD  P MA KLGGEGLI+E+C GFE+LMDK+KG+IT ESLR N S +LG+ D+ ++++ 
Sbjct: 19  FQDFFPTMAGKLGGEGLIEEICKGFELLMDKDKGVITFESLRRNASTVLGLGDLTDDDVR 78

Query: 69  SMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLNWLQYALEREF 114
            M+ EGD D DGALNQMEFCVLMFRLSP+LM+ S   +   +E EF
Sbjct: 79  YMINEGDFDRDGALNQMEFCVLMFRLSPELMEASRCVVTEVIEEEF 124

BLAST of Bhi10G000942 vs. TAIR 10
Match: AT2G46600.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 130.2 bits (326), Expect = 1.1e-30
Identity = 61/109 (55.96%), Postives = 82/109 (75.23%), Query Frame = 0

Query: 4   KVGSNFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTNSWLLGVRDMAE 63
           K+ + ++D+LPVMAEK+  E  + ELC GF +L D E+ LIT ESLR NS +LG+  M++
Sbjct: 17  KMETKYEDMLPVMAEKMDVEEFVSELCKGFSLLADPERHLITAESLRRNSGILGIEGMSK 76

Query: 64  EELLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLNWLQYALERE 113
           E+   M+REGDLDGDGALNQ EFCVLM RLSP++M+ +  WL+ AL +E
Sbjct: 77  EDAQGMVREGDLDGDGALNQTEFCVLMVRLSPEMMEDAETWLEKALTQE 125

BLAST of Bhi10G000942 vs. TAIR 10
Match: AT1G66410.1 (calmodulin 4 )

HSP 1 Score: 49.7 bits (117), Expect = 1.8e-06
Identity = 29/83 (34.94%), Postives = 45/83 (54.22%), Query Frame = 0

Query: 8   NFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTNSWLLGVRDMAEEELL 67
           +F + L +MA+K+      +EL   F V    + G I+   LR     LG + + +EE+ 
Sbjct: 65  DFPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEK-LTDEEVE 124

Query: 68  SMMREGDLDGDGALNQMEFCVLM 91
            M+RE D+DGDG +N  EF  +M
Sbjct: 125 EMIREADVDGDGQINYEEFVKIM 146

BLAST of Bhi10G000942 vs. TAIR 10
Match: AT1G66410.2 (calmodulin 4 )

HSP 1 Score: 49.7 bits (117), Expect = 1.8e-06
Identity = 29/83 (34.94%), Postives = 45/83 (54.22%), Query Frame = 0

Query: 8   NFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTNSWLLGVRDMAEEELL 67
           +F + L +MA+K+      +EL   F V    + G I+   LR     LG + + +EE+ 
Sbjct: 75  DFPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEK-LTDEEVE 134

Query: 68  SMMREGDLDGDGALNQMEFCVLM 91
            M+RE D+DGDG +N  EF  +M
Sbjct: 135 EMIREADVDGDGQINYEEFVKIM 156

BLAST of Bhi10G000942 vs. ExPASy Swiss-Prot
Match: O81831 (Calcium-binding protein KRP1 OS=Arabidopsis thaliana OX=3702 GN=KRP1 PE=2 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 2.7e-31
Identity = 68/107 (63.55%), Postives = 83/107 (77.57%), Query Frame = 0

Query: 8   NFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTN-SWLLGVRDMAEEEL 67
           NF D LP MA  LGGEGLI ELCNGFE+LMD+EKG+IT ESLR N + +LG+ D+ +E++
Sbjct: 18  NFHDFLPTMAGNLGGEGLIGELCNGFELLMDREKGVITFESLRRNAAAVLGLGDLTDEDV 77

Query: 68  LSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLNWLQYALEREF 114
             M++EGD D DGALNQMEFCVLMFRLSPDLM+ S   +   +E EF
Sbjct: 78  RCMIKEGDFDCDGALNQMEFCVLMFRLSPDLMEASRCLVTEVIEEEF 124

BLAST of Bhi10G000942 vs. ExPASy Swiss-Prot
Match: Q9LSQ6 (Calcium-binding protein PBP1 OS=Arabidopsis thaliana OX=3702 GN=PBP1 PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 6.7e-30
Identity = 65/106 (61.32%), Postives = 82/106 (77.36%), Query Frame = 0

Query: 9   FQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTN-SWLLGVRDMAEEELL 68
           FQD  P MA KLGGEGLI+E+C GFE+LMDK+KG+IT ESLR N S +LG+ D+ ++++ 
Sbjct: 19  FQDFFPTMAGKLGGEGLIEEICKGFELLMDKDKGVITFESLRRNASTVLGLGDLTDDDVR 78

Query: 69  SMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLNWLQYALEREF 114
            M+ EGD D DGALNQMEFCVLMFRLSP+LM+ S   +   +E EF
Sbjct: 79  YMINEGDFDRDGALNQMEFCVLMFRLSPELMEASRCVVTEVIEEEF 124

BLAST of Bhi10G000942 vs. ExPASy Swiss-Prot
Match: Q9ZPX9 (Calcium-binding protein KIC OS=Arabidopsis thaliana OX=3702 GN=KIC PE=1 SV=2)

HSP 1 Score: 130.2 bits (326), Expect = 1.5e-29
Identity = 61/109 (55.96%), Postives = 82/109 (75.23%), Query Frame = 0

Query: 4   KVGSNFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTNSWLLGVRDMAE 63
           K+ + ++D+LPVMAEK+  E  + ELC GF +L D E+ LIT ESLR NS +LG+  M++
Sbjct: 17  KMETKYEDMLPVMAEKMDVEEFVSELCKGFSLLADPERHLITAESLRRNSGILGIEGMSK 76

Query: 64  EELLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLNWLQYALERE 113
           E+   M+REGDLDGDGALNQ EFCVLM RLSP++M+ +  WL+ AL +E
Sbjct: 77  EDAQGMVREGDLDGDGALNQTEFCVLMVRLSPEMMEDAETWLEKALTQE 125

BLAST of Bhi10G000942 vs. ExPASy Swiss-Prot
Match: Q2TBN3 (Centrin-2 OS=Bos taurus OX=9913 GN=CETN2 PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 5.6e-08
Identity = 31/87 (35.63%), Postives = 52/87 (59.77%), Query Frame = 0

Query: 8   NFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTNSWLLGVRDMAEEELL 67
           NF D L VM +K+  +   +E+   F++  D E G I+ ++L+  +  LG  ++++EEL 
Sbjct: 85  NFSDFLTVMTQKMSEKDTKEEILKAFKLFDDDETGKISFKNLKRVAKELG-ENLSDEELQ 144

Query: 68  SMMREGDLDGDGALNQMEFCVLMFRLS 95
            M+ E D DGDG +N+ EF  +M + S
Sbjct: 145 EMIDEADRDGDGEVNEQEFLRIMKKTS 170

BLAST of Bhi10G000942 vs. ExPASy Swiss-Prot
Match: Q9R1K9 (Centrin-2 OS=Mus musculus OX=10090 GN=Cetn2 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 7.3e-08
Identity = 31/87 (35.63%), Postives = 51/87 (58.62%), Query Frame = 0

Query: 8   NFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTNSWLLGVRDMAEEELL 67
           NF D L VM +K+  +   +E+   F++  D E G I+ ++L+  +  LG  ++ +EEL 
Sbjct: 85  NFSDFLTVMTQKMSEKDTKEEILKAFKLFDDDETGKISFKNLKRVAKELG-ENLTDEELQ 144

Query: 68  SMMREGDLDGDGALNQMEFCVLMFRLS 95
            M+ E D DGDG +N+ EF  +M + S
Sbjct: 145 EMIDEADRDGDGEVNEQEFLRIMKKTS 170

BLAST of Bhi10G000942 vs. NCBI nr
Match: XP_038902367.1 (calcium-binding protein KRP1-like [Benincasa hispida])

HSP 1 Score: 231.9 bits (590), Expect = 2.8e-57
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0

Query: 1   MARKVGSNFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTNSWLLGVRD 60
           MARKVGSNFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTNSWLLGVRD
Sbjct: 1   MARKVGSNFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTNSWLLGVRD 60

Query: 61  MAEEELLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLNWLQYALEREFN 115
           MAEEELLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLNWLQYALEREFN
Sbjct: 61  MAEEELLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLNWLQYALEREFN 114

BLAST of Bhi10G000942 vs. NCBI nr
Match: XP_004140055.1 (calcium-binding protein KRP1 [Cucumis sativus] >KGN46674.1 hypothetical protein Csa_020739 [Cucumis sativus])

HSP 1 Score: 211.8 bits (538), Expect = 3.0e-51
Identity = 104/113 (92.04%), Postives = 108/113 (95.58%), Query Frame = 0

Query: 1   MARKVGSNFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTNSWLLGVRD 60
           M++K G+NFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKG+ITLESLR NSWLLGVRD
Sbjct: 1   MSKKGGANFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGVITLESLRRNSWLLGVRD 60

Query: 61  MAEEELLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLNWLQYALEREF 114
           MAEEELLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSL WLQ ALE EF
Sbjct: 61  MAEEELLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLYWLQLALENEF 113

BLAST of Bhi10G000942 vs. NCBI nr
Match: XP_023512862.1 (calcium-binding protein PBP1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 182.6 bits (462), Expect = 1.9e-42
Identity = 93/115 (80.87%), Postives = 101/115 (87.83%), Query Frame = 0

Query: 1   MARK--VGSNFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTNSWLLGV 60
           MARK   G  F DLLPVMAEKLGGEGLI ELCNGF++LMDKEKG+ITLESLRTNS LLG+
Sbjct: 1   MARKGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRTNSSLLGL 60

Query: 61  RDMAEEELLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLNWLQYALEREF 114
            DM EEEL+SMMREGDLDGDGALNQMEFCVLMFRLSP+LM+ SL WL  ALE+EF
Sbjct: 61  WDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEF 115

BLAST of Bhi10G000942 vs. NCBI nr
Match: KAG6570861.1 (Calcium-binding protein KRP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 180.3 bits (456), Expect = 9.7e-42
Identity = 92/115 (80.00%), Postives = 100/115 (86.96%), Query Frame = 0

Query: 1   MARK--VGSNFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTNSWLLGV 60
           MARK   G  F DLLPVMAEKLGGEGLI ELCNGF++LMDKEKG+ITLESLR NS LLG+
Sbjct: 1   MARKGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSLLGL 60

Query: 61  RDMAEEELLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLNWLQYALEREF 114
            DM EEEL+SMMREGDLDGDGALNQMEFCVLMFRLSP+LM+ SL WL  ALE+EF
Sbjct: 61  WDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEF 115

BLAST of Bhi10G000942 vs. NCBI nr
Match: XP_022944617.1 (calcium-binding protein PBP1-like [Cucurbita moschata] >KAG7010710.1 Calcium-binding protein PBP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 179.1 bits (453), Expect = 2.2e-41
Identity = 88/108 (81.48%), Postives = 96/108 (88.89%), Query Frame = 0

Query: 6   GSNFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTNSWLLGVRDMAEEE 65
           G  F DLLPVMAEKLGGEGLI ELCNGF++LMDKEKG+ITLESLR NS LLG+ DM EEE
Sbjct: 11  GGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSLLGLWDMGEEE 70

Query: 66  LLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLNWLQYALEREF 114
           L+SMMREGDLDGDGALNQMEFCVLMFRLSP+LM+ SL WL  ALE+EF
Sbjct: 71  LVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEF 118

BLAST of Bhi10G000942 vs. ExPASy TrEMBL
Match: A0A0A0KCV2 (EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G120930 PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.5e-51
Identity = 104/113 (92.04%), Postives = 108/113 (95.58%), Query Frame = 0

Query: 1   MARKVGSNFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTNSWLLGVRD 60
           M++K G+NFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKG+ITLESLR NSWLLGVRD
Sbjct: 1   MSKKGGANFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGVITLESLRRNSWLLGVRD 60

Query: 61  MAEEELLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLNWLQYALEREF 114
           MAEEELLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSL WLQ ALE EF
Sbjct: 61  MAEEELLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLYWLQLALENEF 113

BLAST of Bhi10G000942 vs. ExPASy TrEMBL
Match: A0A6J1JGE3 (calcium-binding protein PBP1-like OS=Cucurbita maxima OX=3661 GN=LOC111484150 PE=4 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 1.0e-41
Identity = 88/108 (81.48%), Postives = 96/108 (88.89%), Query Frame = 0

Query: 6   GSNFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTNSWLLGVRDMAEEE 65
           G  F DLLPVMAEKLGGEGLI ELCNGF++LMDKEKG+ITLESLR NS LLG+ DM EEE
Sbjct: 12  GGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSLLGLWDMGEEE 71

Query: 66  LLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLNWLQYALEREF 114
           L+SMMREGDLDGDGALNQMEFCVLMFRLSP+LM+ SL WL  ALE+EF
Sbjct: 72  LVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEF 119

BLAST of Bhi10G000942 vs. ExPASy TrEMBL
Match: A0A6J1FW37 (calcium-binding protein PBP1-like OS=Cucurbita moschata OX=3662 GN=LOC111449023 PE=4 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 1.0e-41
Identity = 88/108 (81.48%), Postives = 96/108 (88.89%), Query Frame = 0

Query: 6   GSNFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTNSWLLGVRDMAEEE 65
           G  F DLLPVMAEKLGGEGLI ELCNGF++LMDKEKG+ITLESLR NS LLG+ DM EEE
Sbjct: 11  GGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSLLGLWDMGEEE 70

Query: 66  LLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLNWLQYALEREF 114
           L+SMMREGDLDGDGALNQMEFCVLMFRLSP+LM+ SL WL  ALE+EF
Sbjct: 71  LVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEF 118

BLAST of Bhi10G000942 vs. ExPASy TrEMBL
Match: A0A6J1HZZ8 (calcium-binding protein PBP1-like OS=Cucurbita maxima OX=3661 GN=LOC111469641 PE=4 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 7.5e-40
Identity = 88/113 (77.88%), Postives = 98/113 (86.73%), Query Frame = 0

Query: 1   MARKVGSNFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTNSWLLGVRD 60
           MARK  + F DLLPVMAE+LGGEGLI ELCNGF++LMDKEKG+ITL+SLR NS LLG+RD
Sbjct: 1   MARK-SAAFHDLLPVMAEQLGGEGLINELCNGFQLLMDKEKGVITLDSLRLNSSLLGLRD 60

Query: 61  MAEEELLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLNWLQYALEREF 114
           M E ELLSMM EGDLDGDGALNQMEFCVLMFRLSP+LMQHSL  LQ+A   +F
Sbjct: 61  MGEAELLSMMTEGDLDGDGALNQMEFCVLMFRLSPELMQHSLYCLQHASHHQF 112

BLAST of Bhi10G000942 vs. ExPASy TrEMBL
Match: A0A2P5AVV6 (Parvalbumin OS=Trema orientale OX=63057 GN=TorRG33x02_339920 PE=4 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 9.8e-40
Identity = 82/106 (77.36%), Postives = 98/106 (92.45%), Query Frame = 0

Query: 7   SNFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGLITLESLRTNSWLLGVRDMAEEEL 66
           S+F+DLLPVMAE+LGGEGLIKELC+GFE+LMDKE+GLITLESLR N+ +LG++DM E+EL
Sbjct: 25  SDFEDLLPVMAERLGGEGLIKELCSGFELLMDKERGLITLESLRRNAAVLGLQDMREDEL 84

Query: 67  LSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLNWLQYALERE 113
           +SM+REGDLDGDGALNQMEFCVLMFRLSPDLM+ S  WLQ+ L+ E
Sbjct: 85  VSMLREGDLDGDGALNQMEFCVLMFRLSPDLMEDSWFWLQHVLQEE 130

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G27280.11.9e-3263.55Calcium-binding EF-hand family protein [more]
AT5G54490.14.8e-3161.32pinoid-binding protein 1 [more]
AT2G46600.11.1e-3055.96Calcium-binding EF-hand family protein [more]
AT1G66410.11.8e-0634.94calmodulin 4 [more]
AT1G66410.21.8e-0634.94calmodulin 4 [more]
Match NameE-valueIdentityDescription
O818312.7e-3163.55Calcium-binding protein KRP1 OS=Arabidopsis thaliana OX=3702 GN=KRP1 PE=2 SV=1[more]
Q9LSQ66.7e-3061.32Calcium-binding protein PBP1 OS=Arabidopsis thaliana OX=3702 GN=PBP1 PE=1 SV=1[more]
Q9ZPX91.5e-2955.96Calcium-binding protein KIC OS=Arabidopsis thaliana OX=3702 GN=KIC PE=1 SV=2[more]
Q2TBN35.6e-0835.63Centrin-2 OS=Bos taurus OX=9913 GN=CETN2 PE=2 SV=1[more]
Q9R1K97.3e-0835.63Centrin-2 OS=Mus musculus OX=10090 GN=Cetn2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_038902367.12.8e-57100.00calcium-binding protein KRP1-like [Benincasa hispida][more]
XP_004140055.13.0e-5192.04calcium-binding protein KRP1 [Cucumis sativus] >KGN46674.1 hypothetical protein ... [more]
XP_023512862.11.9e-4280.87calcium-binding protein PBP1-like [Cucurbita pepo subsp. pepo][more]
KAG6570861.19.7e-4280.00Calcium-binding protein KRP1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022944617.12.2e-4181.48calcium-binding protein PBP1-like [Cucurbita moschata] >KAG7010710.1 Calcium-bin... [more]
Match NameE-valueIdentityDescription
A0A0A0KCV21.5e-5192.04EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G120930 PE=... [more]
A0A6J1JGE31.0e-4181.48calcium-binding protein PBP1-like OS=Cucurbita maxima OX=3661 GN=LOC111484150 PE... [more]
A0A6J1FW371.0e-4181.48calcium-binding protein PBP1-like OS=Cucurbita moschata OX=3662 GN=LOC111449023 ... [more]
A0A6J1HZZ87.5e-4077.88calcium-binding protein PBP1-like OS=Cucurbita maxima OX=3661 GN=LOC111469641 PE... [more]
A0A2P5AVV69.8e-4077.36Parvalbumin OS=Trema orientale OX=63057 GN=TorRG33x02_339920 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 65..93
e-value: 0.0012
score: 28.1
IPR002048EF-hand domainPFAMPF13833EF-hand_8coord: 40..92
e-value: 1.0E-15
score: 57.3
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 61..96
score: 10.608049
NoneNo IPR availableGENE3D1.10.238.10coord: 6..114
e-value: 1.2E-20
score: 75.5
NoneNo IPR availablePANTHERPTHR47319:SF8CALCIUM-BINDING PROTEIN PBP1-LIKEcoord: 1..113
IPR044205Calcium-binding protein KIC/PBP1/KRP1PANTHERPTHR47319CALCIUM-BINDING PROTEIN KICcoord: 1..113
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 74..86
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 8..90

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi10M000942Bhi10M000942mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding