Bhi10G000937 (gene) Wax gourd (B227) v1

BLAST of Bhi10G000937 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 858.2 bits (2216), Expect = 3.9e-249
Identity = 424/602 (70.43%), Postives = 494/602 (82.06%), Query Frame = 0

Query: 4   EVSNNLTSKDEQSILQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGCTDRKTF 63
           EVS+   ++  ++ LQ IE+VTT+AD VQ RVL EILSRNADVEYLKRHGL G TDR+TF
Sbjct: 11  EVSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETF 70

Query: 64  KNTIPLVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTEEEQKRR 123
           K+ +P+V+YED++P I+RIANGD S +LCS PISEF  SSGTSGGE KL+PT EEE  RR
Sbjct: 71  KHIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRR 130

Query: 124 SLLYFLLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHFKNR--- 183
           SLLY LLMPV++QFVPGLDKGKGMYFLF+KSES TPGGL ARP LTS+ KS HFKNR   
Sbjct: 131 SLLYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYD 190

Query: 184 PYTDYTSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQTHWPLL 243
           PYT+YTSP +TILCSDSYQSMYSQ+LCGLC  K+VLRVG+VFAS FIRAIKFL+ HWP L
Sbjct: 191 PYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPEL 250

Query: 244 VNDIRTGTINNSKITDPLVRESVMNILKPDPLLADFIEFECVKESWGGIIIRLWPNTKYV 303
             DIRTGT+ +S+ITD  VRE+V  ILKPDP LADF+E EC K SW GII RLWPNTKYV
Sbjct: 251 ARDIRTGTL-SSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYV 310

Query: 304 DVIVTGTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLIPSMAYF 363
           DVIVTGTMSQYIPTLDYY+N LP+V +MYASSEC  G+NL PLC P++VSYTLIP+MAYF
Sbjct: 311 DVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYF 370

Query: 364 EFLPTTAHGFSNSS------------QLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDIL 423
           EFLP   +    SS            Q LVDLVDV+LG+EYELVVTTYAGLYRYR+GD+L
Sbjct: 371 EFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVL 430

Query: 424 RVSGFKNNAPQFNFVCRKNIILSIDMDKVEEVELQNAVMEGSKNLIPYGGFVHEYTSYPD 483
            V+GFKNNAPQF+F+CRKN++LSID DK +EVELQNAV     +L+P+   + EYTSY D
Sbjct: 431 SVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYAD 490

Query: 484 IHTIPGHYVIYWEISLKETTTIPPSVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIV 543
             +IPGHYV++WE+ L   T IPPSVFE CCLT+EESLN +YR+GRVS KSIGPLEIK+V
Sbjct: 491 TSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMV 550

Query: 544 ENGTFDKLMDYAINHGSSINQYKTPRCVTKAPILELLNSCVVSNHFSPKCPKWIPGPKEW 591
           E+GTFDKLMDYAI+ G+SINQYKTPRCV  API+ELLNS VV ++FSPKCPKW PG K+W
Sbjct: 551 ESGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHKQW 610

BLAST of Bhi10G000937 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 845.5 bits (2183), Expect = 2.6e-245
Identity = 420/597 (70.35%), Postives = 490/597 (82.08%), Query Frame = 0

Query: 9   LTSKDEQSILQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGCTDRKTFKNTIP 68
           L  K++Q  LQ IEE+T++ADQVQ +VL EIL+RNADVEYL+RH LNG TDR+TFKN +P
Sbjct: 17  LDQKNKQK-LQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMP 76

Query: 69  LVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTEEEQKRRSLLYF 128
           +++YED++P I+RIANGD SPIL S+PISEF  SSGTSGGE KL+PT EEE  RRSLLY 
Sbjct: 77  VITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYS 136

Query: 129 LLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHFKNR---PYTDY 188
           LLMPV++QFVPGL+ GKGMYFLF+KSES TPGGL ARP LTS+ KS HFK R   PYT+Y
Sbjct: 137 LLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNY 196

Query: 189 TSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQTHWPLLVNDIR 248
           TSP ETILCSDSYQSMYSQ+LCGLC  ++VLRVG+VFAS FIRAIKFL+ HW  LV DIR
Sbjct: 197 TSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIR 256

Query: 249 TGTINNSKITDPLVRESVMNILKPDPLLADFIEFECVKESWGGIIIRLWPNTKYVDVIVT 308
           TGT+ +S ITDP VRE+V  ILKP P LADF+EFEC K SW GII RLWPNTKYVDVIVT
Sbjct: 257 TGTL-SSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVT 316

Query: 309 GTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLIPSMAYFEFLPT 368
           GTMSQYIPTLDYY+N LP+V +MYASSEC  G+NL PLC P++VSYTLIPSMAYFEFLP 
Sbjct: 317 GTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPV 376

Query: 369 -TAHGFSNS-----------SQLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDILRVSGF 428
              +G +NS            Q LVDLVDV+LG+EYELVVTTYAGL RYR+GD+LRV+GF
Sbjct: 377 HRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGF 436

Query: 429 KNNAPQFNFVCRKNIILSIDMDKVEEVELQNAVMEGSKNLIPYGGFVHEYTSYPDIHTIP 488
           KN APQF+F+CRKN++LSID DK +EVELQNAV     +L+P+   + EYTSY D  +IP
Sbjct: 437 KNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIP 496

Query: 489 GHYVIYWEISLKETTTIPPSVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIVENGTF 548
           GHYV++WE+ L   T IPPSVFE CCL +EES N +YR+GRVS KSIGPLEIKIVE GTF
Sbjct: 497 GHYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTF 556

Query: 549 DKLMDYAINHGSSINQYKTPRCVTKAPILELLNSCVVSNHFSPKCPKWIPGPKEWST 591
           DKLMDYAI+ G+SINQYKTPRCV  API+ELLNS VV ++FSPKCPKW+PG K+W +
Sbjct: 557 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVPGHKQWGS 611

BLAST of Bhi10G000937 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 699.1 bits (1803), Expect = 3.0e-201
Identity = 354/591 (59.90%), Postives = 437/591 (73.94%), Query Frame = 0

Query: 5   VSNNLTS-------KDEQSILQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGC 64
           V +NL+S       + +   L+ IEE+T +AD VQ  +L EIL+RNAD EYL+R  L G 
Sbjct: 3   VDSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGA 62

Query: 65  TDRKTFKNTIPLVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTE 124
           TDR TFK  IP+++YEDL+P I RIA+GD SPIL + PISEF  SSGTS GE KL+PT +
Sbjct: 63  TDRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIK 122

Query: 125 EEQKRRSLLYFLLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHF 184
           EE  RR LLY LLMPV+N +VPGLDKGKGMYFLFVKSE+ TPGGL ARP LTS+ KS HF
Sbjct: 123 EELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHF 182

Query: 185 KNRPYTD---YTSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQ 244
           ++RPY     YTSP E ILC DS+QSMY+Q+LCGL     VLRVG+VFAS  +RAI+FLQ
Sbjct: 183 RSRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQ 242

Query: 245 THWPLLVNDIRTGTINNSKITDPLVRESVMNILKPDPLLADFIEFECVKESWGGIIIRLW 304
            HW    +DI  G + +S+ITDP +R+ +  ILKPDP+LA+FI  EC  ++W  II R+W
Sbjct: 243 LHWSRFAHDIELGCL-DSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIW 302

Query: 305 PNTKYVDVIVTGTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLI 364
           PNTKY+DVIVTG M+QYIPTL+YY+  LP+  +MYASSEC  G+NLNP+  P++VSYT++
Sbjct: 303 PNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIM 362

Query: 365 PSMAYFEFLPTTAHGFSNSSQLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDILRVSGFK 424
           P+MAYFEF+P             V+LVDV +GKEYELVVTTYAGL RYR+GDILRV+GF 
Sbjct: 363 PNMAYFEFIPL-------GGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFH 422

Query: 425 NNAPQFNFVCRKNIILSIDMDKVEEVELQNAVMEGSKNL-IPYGGFVHEYTSYPDIHTIP 484
           N+APQF+FV RKN++LSID DK +E ELQ AV   S  L    G  V EYTSY D  TIP
Sbjct: 423 NSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIP 482

Query: 485 GHYVIYWEISLKETTTIPP-SVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIVENGT 544
           GHYV+YWE+ +++    P      +CCL MEESLN +YR+ RV+  S+GPLEI++V NGT
Sbjct: 483 GHYVLYWELLVRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGT 542

Query: 545 FDKLMDYAINHGSSINQYKTPRCVTKAPILELLNSCVVSNHFSPKCPKWIP 584
           F++LMDYAI+ G+SINQYK PRCV   PI+ELL+S VVS HFSP  P W P
Sbjct: 543 FEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTP 585

BLAST of Bhi10G000937 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 698.7 bits (1802), Expect = 3.9e-201
Identity = 357/589 (60.61%), Postives = 447/589 (75.89%), Query Frame = 0

Query: 1   MRTEVSNNLTSKDEQSILQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGCTDR 60
           +R+ + ++ ++KD ++ L+ IEE+T + D VQ +V+ EILSRN+D EYLKR GL G TDR
Sbjct: 7   LRSPMMHSPSTKDVKA-LRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDR 66

Query: 61  KTFKNTIPLVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTEEEQ 120
           KTFK  +P+V Y+DLKP I RIANGD S IL S PI+EF  SSGTS GE KL+PT +E+ 
Sbjct: 67  KTFKTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDM 126

Query: 121 KRRSLLYFLLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHFKNR 180
            RR LLY LLMPV+N +VPGLDKGK +YFLFVK+ES TPGGL ARP LTS+ KS  FK R
Sbjct: 127 DRRQLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRR 186

Query: 181 ---PYTDYTSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQTHW 240
              PY  YTSP E ILC DS QSMY+Q+LCGL +  +VLR+G+VFAS  +RAI FLQT+W
Sbjct: 187 PNDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNW 246

Query: 241 PLLVNDIRTGTINNSKITDPLVRESVMNIL-KPDPLLADFIEFECVKE-SWGGIIIRLWP 300
             L +DI TGT+ +S+I+DP ++ES+  IL KPD  LADFI   C ++ SW GII ++WP
Sbjct: 247 KELADDISTGTL-SSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWP 306

Query: 301 NTKYVDVIVTGTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLIP 360
           NTKY+DVIVTG M+QYIP L+YY+  LP+  +MYASSE   GINL P+C P++VSYT++P
Sbjct: 307 NTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMP 366

Query: 361 SMAYFEFLPTTAHGFSNSSQLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDILRVSGFKN 420
           +MAYFEFLP   H        LV+L DV++GKEYELV+TTYAGL RYR+GDIL+V+GF N
Sbjct: 367 NMAYFEFLP--HHEVPTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYN 426

Query: 421 NAPQFNFVCRKNIILSIDMDKVEEVELQNAVMEGSKNLIPYGGFVHEYTSYPDIHTIPGH 480
           +APQF FV RKN++LSI+ DK +E ELQ+AV   S  L   G  V EYTSY +  TIPGH
Sbjct: 427 SAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGH 486

Query: 481 YVIYWEISLKETTTIP-PSVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIVENGTFD 540
           YVIYWE+ +K+ T  P   V  +CCL MEESLN +YR+ RV+ KSIGPLEI++V+NGTF+
Sbjct: 487 YVIYWELLVKDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFE 546

Query: 541 KLMDYAINHGSSINQYKTPRCVTKAPILELLNSCVVSNHFSPKCPKWIP 584
           +LMDYAI+ G+SINQYK PRCV+  PI+ELL+S VVS HFSP  P W P
Sbjct: 547 ELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSP 591

BLAST of Bhi10G000937 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 697.6 bits (1799), Expect = 8.7e-201
Identity = 350/594 (58.92%), Postives = 443/594 (74.58%), Query Frame = 0

Query: 1   MRTEVSNNLTSKDEQSILQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGCTDR 60
           +++ + +  TS+ +   L+ IEE+T + D VQ +VL EIL+RN++ EYLKR  L+G  DR
Sbjct: 7   LQSRMVSATTSEKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDR 66

Query: 61  KTFKNTIPLVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTEEEQ 120
           KTFK+ +P+V+YEDLKP I RI+NGD SPIL S PI+EF  SSGTS GE KL+PT EE+ 
Sbjct: 67  KTFKSKVPVVTYEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDL 126

Query: 121 KRRSLLYFLLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHFKNR 180
            RR LLY LLMPV+N +VPGLDKGKG+YFLFVKSES T GGL ARP LTS+ KS HFK R
Sbjct: 127 DRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRR 186

Query: 181 PYTD---YTSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQTHW 240
           PY     YTSP E ILCSDS QSMY+Q+LCGL +  +VLR+G+VFAS  +RAI FLQ +W
Sbjct: 187 PYDPYNVYTSPNEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNW 246

Query: 241 PLLVNDIRTGTINNSKITDPLVRESVMNIL-KPDPLLADFIEFECVKESWGGIIIRLWPN 300
             L  DI TGT+ +S+I DP ++  +  IL KPD  LA+F+   C +E+W GII ++WPN
Sbjct: 247 KELARDISTGTL-SSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPN 306

Query: 301 TKYVDVIVTGTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLIPS 360
           TKY+DVIVTG M+QYIPTL+YY+  LP+  +MYASSE   GINL P+C P++VSYT++P+
Sbjct: 307 TKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPN 366

Query: 361 MAYFEFLPTTAHG-----FSNSSQLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDILRVS 420
           MAYFEFLP    G      S     LV+L +V++GKEYELV+TTYAGLYRYR+GDILRV+
Sbjct: 367 MAYFEFLPHNHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVT 426

Query: 421 GFKNNAPQFNFVCRKNIILSIDMDKVEEVELQNAVMEGSKNLIPYGGFVHEYTSYPDIHT 480
           GF N+APQF F+ RKN++LS++ DK +E ELQ AV   S+     G  V EYTSY +  T
Sbjct: 427 GFHNSAPQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKT 486

Query: 481 IPGHYVIYWEISLKETTT--IPPSVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIVE 540
           IPGHYVIYWE+  ++ +   +   V  +CCL MEESLN +YR+ RV+ KSIGPLEI++V 
Sbjct: 487 IPGHYVIYWELLGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVR 546

Query: 541 NGTFDKLMDYAINHGSSINQYKTPRCVTKAPILELLNSCVVSNHFSPKCPKWIP 584
           NGTF++LMDYAI+ G+SINQYK PRCV+  PI+ELL+S VVS HFSP  P W P
Sbjct: 547 NGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSP 599

BLAST of Bhi10G000937 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 858.2 bits (2216), Expect = 5.4e-248
Identity = 424/602 (70.43%), Postives = 494/602 (82.06%), Query Frame = 0

Query: 4   EVSNNLTSKDEQSILQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGCTDRKTF 63
           EVS+   ++  ++ LQ IE+VTT+AD VQ RVL EILSRNADVEYLKRHGL G TDR+TF
Sbjct: 11  EVSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETF 70

Query: 64  KNTIPLVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTEEEQKRR 123
           K+ +P+V+YED++P I+RIANGD S +LCS PISEF  SSGTSGGE KL+PT EEE  RR
Sbjct: 71  KHIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRR 130

Query: 124 SLLYFLLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHFKNR--- 183
           SLLY LLMPV++QFVPGLDKGKGMYFLF+KSES TPGGL ARP LTS+ KS HFKNR   
Sbjct: 131 SLLYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYD 190

Query: 184 PYTDYTSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQTHWPLL 243
           PYT+YTSP +TILCSDSYQSMYSQ+LCGLC  K+VLRVG+VFAS FIRAIKFL+ HWP L
Sbjct: 191 PYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPEL 250

Query: 244 VNDIRTGTINNSKITDPLVRESVMNILKPDPLLADFIEFECVKESWGGIIIRLWPNTKYV 303
             DIRTGT+ +S+ITD  VRE+V  ILKPDP LADF+E EC K SW GII RLWPNTKYV
Sbjct: 251 ARDIRTGTL-SSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYV 310

Query: 304 DVIVTGTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLIPSMAYF 363
           DVIVTGTMSQYIPTLDYY+N LP+V +MYASSEC  G+NL PLC P++VSYTLIP+MAYF
Sbjct: 311 DVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYF 370

Query: 364 EFLPTTAHGFSNSS------------QLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDIL 423
           EFLP   +    SS            Q LVDLVDV+LG+EYELVVTTYAGLYRYR+GD+L
Sbjct: 371 EFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVL 430

Query: 424 RVSGFKNNAPQFNFVCRKNIILSIDMDKVEEVELQNAVMEGSKNLIPYGGFVHEYTSYPD 483
            V+GFKNNAPQF+F+CRKN++LSID DK +EVELQNAV     +L+P+   + EYTSY D
Sbjct: 431 SVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYAD 490

Query: 484 IHTIPGHYVIYWEISLKETTTIPPSVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIV 543
             +IPGHYV++WE+ L   T IPPSVFE CCLT+EESLN +YR+GRVS KSIGPLEIK+V
Sbjct: 491 TSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMV 550

Query: 544 ENGTFDKLMDYAINHGSSINQYKTPRCVTKAPILELLNSCVVSNHFSPKCPKWIPGPKEW 591
           E+GTFDKLMDYAI+ G+SINQYKTPRCV  API+ELLNS VV ++FSPKCPKW PG K+W
Sbjct: 551 ESGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHKQW 610

BLAST of Bhi10G000937 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 3.6e-244
Identity = 420/597 (70.35%), Postives = 490/597 (82.08%), Query Frame = 0

Query: 9   LTSKDEQSILQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGCTDRKTFKNTIP 68
           L  K++Q  LQ IEE+T++ADQVQ +VL EIL+RNADVEYL+RH LNG TDR+TFKN +P
Sbjct: 17  LDQKNKQK-LQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMP 76

Query: 69  LVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTEEEQKRRSLLYF 128
           +++YED++P I+RIANGD SPIL S+PISEF  SSGTSGGE KL+PT EEE  RRSLLY 
Sbjct: 77  VITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYS 136

Query: 129 LLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHFKNR---PYTDY 188
           LLMPV++QFVPGL+ GKGMYFLF+KSES TPGGL ARP LTS+ KS HFK R   PYT+Y
Sbjct: 137 LLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNY 196

Query: 189 TSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQTHWPLLVNDIR 248
           TSP ETILCSDSYQSMYSQ+LCGLC  ++VLRVG+VFAS FIRAIKFL+ HW  LV DIR
Sbjct: 197 TSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIR 256

Query: 249 TGTINNSKITDPLVRESVMNILKPDPLLADFIEFECVKESWGGIIIRLWPNTKYVDVIVT 308
           TGT+ +S ITDP VRE+V  ILKP P LADF+EFEC K SW GII RLWPNTKYVDVIVT
Sbjct: 257 TGTL-SSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVT 316

Query: 309 GTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLIPSMAYFEFLPT 368
           GTMSQYIPTLDYY+N LP+V +MYASSEC  G+NL PLC P++VSYTLIPSMAYFEFLP 
Sbjct: 317 GTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPV 376

Query: 369 -TAHGFSNS-----------SQLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDILRVSGF 428
              +G +NS            Q LVDLVDV+LG+EYELVVTTYAGL RYR+GD+LRV+GF
Sbjct: 377 HRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGF 436

Query: 429 KNNAPQFNFVCRKNIILSIDMDKVEEVELQNAVMEGSKNLIPYGGFVHEYTSYPDIHTIP 488
           KN APQF+F+CRKN++LSID DK +EVELQNAV     +L+P+   + EYTSY D  +IP
Sbjct: 437 KNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIP 496

Query: 489 GHYVIYWEISLKETTTIPPSVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIVENGTF 548
           GHYV++WE+ L   T IPPSVFE CCL +EES N +YR+GRVS KSIGPLEIKIVE GTF
Sbjct: 497 GHYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTF 556

Query: 549 DKLMDYAINHGSSINQYKTPRCVTKAPILELLNSCVVSNHFSPKCPKWIPGPKEWST 591
           DKLMDYAI+ G+SINQYKTPRCV  API+ELLNS VV ++FSPKCPKW+PG K+W +
Sbjct: 557 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVPGHKQWGS 611

BLAST of Bhi10G000937 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 6.3e-204
Identity = 353/578 (61.07%), Postives = 438/578 (75.78%), Query Frame = 0

Query: 18  LQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGCTD-RKTFKNTIPLVSYEDLK 77
           L+ IE VT +A QVQ RVL EIL++NA  EYL+R+G+ G  D    F+  +PLV+YE L+
Sbjct: 24  LEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEGLQ 83

Query: 78  PYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTEEEQKRRSLLYFLLMPVINQ 137
           P I RIANGD SPI   +PISEF  SSGTSGGE KL+PT  +E  RRSLLY LLMPV++Q
Sbjct: 84  PDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVMSQ 143

Query: 138 FVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHFKNR---PYTDYTSPIETIL 197
            V GLDKGK MY LFVK+ES TPGGL ARP LTS+ +S  F +R   PYT YTSP E IL
Sbjct: 144 SVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDEAIL 203

Query: 198 CSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQTHWPLLVNDIRTGTINNSK 257
           C DSYQSMY+QLLCGL     VLRVG+VFAS F+RAI FL+ HW  L +DIRTG + + +
Sbjct: 204 CVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGEL-DPE 263

Query: 258 ITDPLVRESVMNILKPDPLLADFIEFECVKESWGGIIIRLWPNTKYVDVIVTGTMSQYIP 317
           ITD +VR++V  +L+ DP LAD IE EC + SW GII RLWP TKY+DVIVTGTMSQYIP
Sbjct: 264 ITDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQYIP 323

Query: 318 TLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLIPSMAYFEFLPTTAHGFS-- 377
           TL++Y   LP+  +MYASSEC  G+NLNP+C P+ V+YTLIP+M Y+EFLP   +  +  
Sbjct: 324 TLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCNNATAE 383

Query: 378 NSSQLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDILRVSGFKNNAPQFNFVCRKNIILS 437
            S + LVDLVDV+LG EYELVVTTY+GLYRYR+GD+LRV+GFKN AP F+FV R+N+ LS
Sbjct: 384 ASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSFVRRQNVALS 443

Query: 438 IDMDKVEEVELQNAVMEGSKNLIPYGGFVHEYTSYPDIHTIPGHYVIYWEISLKETTTIP 497
           +D DK +E EL  AV    ++L P+G  + EYTSY D  TIPGHYV++WE+    +T +P
Sbjct: 444 VDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWELR-AGSTAVP 503

Query: 498 PSVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIVENGTFDKLMDYAINHGSSINQYK 557
            SVFE+CCL++EE+LN +YR+GR   +SIGPLEI++V  GTFDKLMDYAI+ G+SINQYK
Sbjct: 504 ASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDYAISRGASINQYK 563

Query: 558 TPRCVTKAPILELLNSCVVSNHFSPKCPKWIPGPKEWS 590
            PRCV   P++ELL++ V   +FSPKCPKW PG K+W+
Sbjct: 564 APRCVRPGPVVELLDARVQGKYFSPKCPKWSPGNKQWN 599

BLAST of Bhi10G000937 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 4.2e-200
Identity = 354/591 (59.90%), Postives = 437/591 (73.94%), Query Frame = 0

Query: 5   VSNNLTS-------KDEQSILQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGC 64
           V +NL+S       + +   L+ IEE+T +AD VQ  +L EIL+RNAD EYL+R  L G 
Sbjct: 3   VDSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGA 62

Query: 65  TDRKTFKNTIPLVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTE 124
           TDR TFK  IP+++YEDL+P I RIA+GD SPIL + PISEF  SSGTS GE KL+PT +
Sbjct: 63  TDRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIK 122

Query: 125 EEQKRRSLLYFLLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHF 184
           EE  RR LLY LLMPV+N +VPGLDKGKGMYFLFVKSE+ TPGGL ARP LTS+ KS HF
Sbjct: 123 EELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHF 182

Query: 185 KNRPYTD---YTSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQ 244
           ++RPY     YTSP E ILC DS+QSMY+Q+LCGL     VLRVG+VFAS  +RAI+FLQ
Sbjct: 183 RSRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQ 242

Query: 245 THWPLLVNDIRTGTINNSKITDPLVRESVMNILKPDPLLADFIEFECVKESWGGIIIRLW 304
            HW    +DI  G + +S+ITDP +R+ +  ILKPDP+LA+FI  EC  ++W  II R+W
Sbjct: 243 LHWSRFAHDIELGCL-DSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIW 302

Query: 305 PNTKYVDVIVTGTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLI 364
           PNTKY+DVIVTG M+QYIPTL+YY+  LP+  +MYASSEC  G+NLNP+  P++VSYT++
Sbjct: 303 PNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIM 362

Query: 365 PSMAYFEFLPTTAHGFSNSSQLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDILRVSGFK 424
           P+MAYFEF+P             V+LVDV +GKEYELVVTTYAGL RYR+GDILRV+GF 
Sbjct: 363 PNMAYFEFIPL-------GGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFH 422

Query: 425 NNAPQFNFVCRKNIILSIDMDKVEEVELQNAVMEGSKNL-IPYGGFVHEYTSYPDIHTIP 484
           N+APQF+FV RKN++LSID DK +E ELQ AV   S  L    G  V EYTSY D  TIP
Sbjct: 423 NSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIP 482

Query: 485 GHYVIYWEISLKETTTIPP-SVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIVENGT 544
           GHYV+YWE+ +++    P      +CCL MEESLN +YR+ RV+  S+GPLEI++V NGT
Sbjct: 483 GHYVLYWELLVRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGT 542

Query: 545 FDKLMDYAINHGSSINQYKTPRCVTKAPILELLNSCVVSNHFSPKCPKWIP 584
           F++LMDYAI+ G+SINQYK PRCV   PI+ELL+S VVS HFSP  P W P
Sbjct: 543 FEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTP 585

BLAST of Bhi10G000937 vs. ExPASy Swiss-Prot
Match: O22190 (Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=GH3.3 PE=1 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 5.5e-200
Identity = 357/589 (60.61%), Postives = 447/589 (75.89%), Query Frame = 0

Query: 1   MRTEVSNNLTSKDEQSILQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGCTDR 60
           +R+ + ++ ++KD ++ L+ IEE+T + D VQ +V+ EILSRN+D EYLKR GL G TDR
Sbjct: 7   LRSPMMHSPSTKDVKA-LRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDR 66

Query: 61  KTFKNTIPLVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTEEEQ 120
           KTFK  +P+V Y+DLKP I RIANGD S IL S PI+EF  SSGTS GE KL+PT +E+ 
Sbjct: 67  KTFKTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDM 126

Query: 121 KRRSLLYFLLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHFKNR 180
            RR LLY LLMPV+N +VPGLDKGK +YFLFVK+ES TPGGL ARP LTS+ KS  FK R
Sbjct: 127 DRRQLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRR 186

Query: 181 ---PYTDYTSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQTHW 240
              PY  YTSP E ILC DS QSMY+Q+LCGL +  +VLR+G+VFAS  +RAI FLQT+W
Sbjct: 187 PNDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNW 246

Query: 241 PLLVNDIRTGTINNSKITDPLVRESVMNIL-KPDPLLADFIEFECVKE-SWGGIIIRLWP 300
             L +DI TGT+ +S+I+DP ++ES+  IL KPD  LADFI   C ++ SW GII ++WP
Sbjct: 247 KELADDISTGTL-SSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWP 306

Query: 301 NTKYVDVIVTGTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLIP 360
           NTKY+DVIVTG M+QYIP L+YY+  LP+  +MYASSE   GINL P+C P++VSYT++P
Sbjct: 307 NTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMP 366

Query: 361 SMAYFEFLPTTAHGFSNSSQLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDILRVSGFKN 420
           +MAYFEFLP   H        LV+L DV++GKEYELV+TTYAGL RYR+GDIL+V+GF N
Sbjct: 367 NMAYFEFLP--HHEVPTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYN 426

Query: 421 NAPQFNFVCRKNIILSIDMDKVEEVELQNAVMEGSKNLIPYGGFVHEYTSYPDIHTIPGH 480
           +APQF FV RKN++LSI+ DK +E ELQ+AV   S  L   G  V EYTSY +  TIPGH
Sbjct: 427 SAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGH 486

Query: 481 YVIYWEISLKETTTIP-PSVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIVENGTFD 540
           YVIYWE+ +K+ T  P   V  +CCL MEESLN +YR+ RV+ KSIGPLEI++V+NGTF+
Sbjct: 487 YVIYWELLVKDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFE 546

Query: 541 KLMDYAINHGSSINQYKTPRCVTKAPILELLNSCVVSNHFSPKCPKWIP 584
           +LMDYAI+ G+SINQYK PRCV+  PI+ELL+S VVS HFSP  P W P
Sbjct: 547 ELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSP 591

BLAST of Bhi10G000937 vs. NCBI nr
Match: XP_038901036.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Benincasa hispida])

HSP 1 Score: 1209.9 bits (3129), Expect = 0.0e+00
Identity = 592/592 (100.00%), Postives = 592/592 (100.00%), Query Frame = 0

Query: 1   MRTEVSNNLTSKDEQSILQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGCTDR 60
           MRTEVSNNLTSKDEQSILQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGCTDR
Sbjct: 1   MRTEVSNNLTSKDEQSILQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGCTDR 60

Query: 61  KTFKNTIPLVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTEEEQ 120
           KTFKNTIPLVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTEEEQ
Sbjct: 61  KTFKNTIPLVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTEEEQ 120

Query: 121 KRRSLLYFLLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHFKNR 180
           KRRSLLYFLLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHFKNR
Sbjct: 121 KRRSLLYFLLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHFKNR 180

Query: 181 PYTDYTSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQTHWPLL 240
           PYTDYTSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQTHWPLL
Sbjct: 181 PYTDYTSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQTHWPLL 240

Query: 241 VNDIRTGTINNSKITDPLVRESVMNILKPDPLLADFIEFECVKESWGGIIIRLWPNTKYV 300
           VNDIRTGTINNSKITDPLVRESVMNILKPDPLLADFIEFECVKESWGGIIIRLWPNTKYV
Sbjct: 241 VNDIRTGTINNSKITDPLVRESVMNILKPDPLLADFIEFECVKESWGGIIIRLWPNTKYV 300

Query: 301 DVIVTGTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLIPSMAYF 360
           DVIVTGTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLIPSMAYF
Sbjct: 301 DVIVTGTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLIPSMAYF 360

Query: 361 EFLPTTAHGFSNSSQLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDILRVSGFKNNAPQF 420
           EFLPTTAHGFSNSSQLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDILRVSGFKNNAPQF
Sbjct: 361 EFLPTTAHGFSNSSQLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDILRVSGFKNNAPQF 420

Query: 421 NFVCRKNIILSIDMDKVEEVELQNAVMEGSKNLIPYGGFVHEYTSYPDIHTIPGHYVIYW 480
           NFVCRKNIILSIDMDKVEEVELQNAVMEGSKNLIPYGGFVHEYTSYPDIHTIPGHYVIYW
Sbjct: 421 NFVCRKNIILSIDMDKVEEVELQNAVMEGSKNLIPYGGFVHEYTSYPDIHTIPGHYVIYW 480

Query: 481 EISLKETTTIPPSVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIVENGTFDKLMDYA 540
           EISLKETTTIPPSVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIVENGTFDKLMDYA
Sbjct: 481 EISLKETTTIPPSVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIVENGTFDKLMDYA 540

Query: 541 INHGSSINQYKTPRCVTKAPILELLNSCVVSNHFSPKCPKWIPGPKEWSTEN 593
           INHGSSINQYKTPRCVTKAPILELLNSCVVSNHFSPKCPKWIPGPKEWSTEN
Sbjct: 541 INHGSSINQYKTPRCVTKAPILELLNSCVVSNHFSPKCPKWIPGPKEWSTEN 592

BLAST of Bhi10G000937 vs. NCBI nr
Match: XP_022970970.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima])

HSP 1 Score: 949.5 bits (2453), Expect = 1.4e-272
Identity = 462/586 (78.84%), Postives = 514/586 (87.71%), Query Frame = 0

Query: 9   LTSKDEQSILQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGCTDRKTFKNTIP 68
           +  KDE ++LQHIEE+TTHADQVQ RVLTEILSRNA VEYL++ GL+GC DR  FKN +P
Sbjct: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60

Query: 69  LVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTEEEQKRRSLLYF 128
           +VSYE+LKPYIDRIANGDASPILCS+PISEF  SSGTSGGE KL+PTT+EE KRRS LY 
Sbjct: 61  VVSYENLKPYIDRIANGDASPILCSEPISEFLTSSGTSGGERKLMPTTKEELKRRSFLYS 120

Query: 129 LLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHFKNRP---YTDY 188
           LLMPV+NQF+PGL+KGKGMYFLFVKSES TPGGL ARP LTS+ KS HFK+RP   YTDY
Sbjct: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180

Query: 189 TSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQTHWPLLVNDIR 248
           TSP +TILC+DSYQSMYSQ LCGLCLS+QVLRVGSVFAS FIRAIKFL+THW LL NDIR
Sbjct: 181 TSPNQTILCTDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240

Query: 249 TGTINNSKITDPLVRESVMNILKPDPLLADFIEFECVKESWGGIIIRLWPNTKYVDVIVT 308
           TGTI N +I+DPLVR+SVMNILKPDP+LAD IEFEC K +W GII RLWPNTKYVDVIVT
Sbjct: 241 TGTI-NPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIITRLWPNTKYVDVIVT 300

Query: 309 GTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLIPSMAYFEFLPT 368
           GTMSQYIPTLD Y+N LP+V +MYASSEC  G+NLNPLCNPN+VSYTLIP+MAYFEFLP 
Sbjct: 301 GTMSQYIPTLDKYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPL 360

Query: 369 TAHGFSNSSQLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDILRVSGFKNNAPQFNFVCR 428
                   S+ LVDLVDV+LGKEYEL++TTY+GLYRYR+GDILRVSGFKN APQFNFVCR
Sbjct: 361 D----RKMSEELVDLVDVELGKEYELIITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCR 420

Query: 429 KNIILSIDMDKVEEVELQNAVMEGSKNLIPYGGFVHEYTSYPDIHTIPGHYVIYWEISLK 488
           KN+ILSID DKV+EVELQNAVME SKNLIPY   + EYTSY D HT+PGHYVIYWEIS K
Sbjct: 421 KNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQK 480

Query: 489 ETTTIPPSVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIVENGTFDKLMDYAINHGS 548
           ET  IP  VFE CCLT+EESLN +YR+GRVS KSIGPLE+KI+ENGTFDKLMDYAINHGS
Sbjct: 481 ETNAIPDWVFENCCLTIEESLNSVYRQGRVSDKSIGPLELKIIENGTFDKLMDYAINHGS 540

Query: 549 SINQYKTPRCVTKAPILELLNSCVVSNHFSPKCPKWIPGPKEWSTE 592
           SINQYKTPRCV + PILELLNS VVSN+FSPKCPKW+PG KEWST+
Sbjct: 541 SINQYKTPRCVKREPILELLNSYVVSNYFSPKCPKWVPGQKEWSTK 581

BLAST of Bhi10G000937 vs. NCBI nr
Match: XP_022948165.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 949.5 bits (2453), Expect = 1.4e-272
Identity = 462/586 (78.84%), Postives = 512/586 (87.37%), Query Frame = 0

Query: 9   LTSKDEQSILQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGCTDRKTFKNTIP 68
           +  KDE ++LQHIEE+TTHADQVQ RVLTEILSRNA VEYL++ GL+GC DR  FKN +P
Sbjct: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60

Query: 69  LVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTEEEQKRRSLLYF 128
           +VSYE+LKPYIDRIANGDASPILCS+P+SEF  SSGTSGGE KL+PTTEEE KRRS LY 
Sbjct: 61  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 120

Query: 129 LLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHFKNRP---YTDY 188
           LLMPV+NQF+PGL+KGKGMYFLFVKSES TPGGL ARP LTS+ KS HFK+RP   YTDY
Sbjct: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180

Query: 189 TSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQTHWPLLVNDIR 248
           TSP +TILC+DSYQSMY Q LCGLCLS+QVLRVGSVFAS FIRAIKFL+THW LL NDIR
Sbjct: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240

Query: 249 TGTINNSKITDPLVRESVMNILKPDPLLADFIEFECVKESWGGIIIRLWPNTKYVDVIVT 308
           TGTI N +I DPLVR+SVMNILKPDP+LAD IEFEC K +W GII RLWPNTKY+DVIVT
Sbjct: 241 TGTI-NPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVT 300

Query: 309 GTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLIPSMAYFEFLPT 368
           GTMSQYIPTLDYY+N LP+V +MYASSEC  G+NLNPLCNPN+VSYTLIP+MAYFEFLP 
Sbjct: 301 GTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPL 360

Query: 369 TAHGFSNSSQLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDILRVSGFKNNAPQFNFVCR 428
                   S+ LVDLVDV+LGKEYELV+TTY+GLYRYR+GDILRVSGFKN APQFNFVCR
Sbjct: 361 D----RKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCR 420

Query: 429 KNIILSIDMDKVEEVELQNAVMEGSKNLIPYGGFVHEYTSYPDIHTIPGHYVIYWEISLK 488
           KN+ILSID DKV+EVELQNAVME SKNLIPY   + EYTSY D HT+PGHYVIYWEIS K
Sbjct: 421 KNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQK 480

Query: 489 ETTTIPPSVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIVENGTFDKLMDYAINHGS 548
           ET  IP  VFE CCLT+EESLN +YR+GRVS KSIGPLEIKI+ENGTFDKLMDYAINHGS
Sbjct: 481 ETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGS 540

Query: 549 SINQYKTPRCVTKAPILELLNSCVVSNHFSPKCPKWIPGPKEWSTE 592
           SINQYKTPRCV   PILELLNS VVSN+FSP+CPKW+PG KEWST+
Sbjct: 541 SINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWSTK 581

BLAST of Bhi10G000937 vs. NCBI nr
Match: KAG6604833.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 948.7 bits (2451), Expect = 2.3e-272
Identity = 461/586 (78.67%), Postives = 513/586 (87.54%), Query Frame = 0

Query: 9   LTSKDEQSILQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGCTDRKTFKNTIP 68
           +  KDE ++LQHIEE+TTHADQVQ RVLTEILSRNA VEYL++ GL+GC DR  FKN +P
Sbjct: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60

Query: 69  LVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTEEEQKRRSLLYF 128
           +VSYE+LKPYIDRIANGDASPILCS+P+SEF  SSGTSGGE KL+PTT+EE KRRS LY 
Sbjct: 61  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTKEELKRRSFLYS 120

Query: 129 LLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHFKNRP---YTDY 188
           LLMPV+NQF+PGL+KGKGMYFLFVKSES TPGGL ARP LTS+ KS HFK+RP   YTDY
Sbjct: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180

Query: 189 TSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQTHWPLLVNDIR 248
           TSP +TILC+DSYQSMY Q LCGLCLS+QVLRVGSVFAS FIRAIKFL+THW LL NDIR
Sbjct: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240

Query: 249 TGTINNSKITDPLVRESVMNILKPDPLLADFIEFECVKESWGGIIIRLWPNTKYVDVIVT 308
           TGTI N +I+DPLVR+SVMNILKPDP+LAD IEFEC K +W GII RLWPNTKY+DVIVT
Sbjct: 241 TGTI-NPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVT 300

Query: 309 GTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLIPSMAYFEFLPT 368
           GTMSQYIPTLDYY+N LP+V +MYASSEC  G+NLNPLCNPN+VSYTLIP+MAYFEFLP 
Sbjct: 301 GTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPL 360

Query: 369 TAHGFSNSSQLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDILRVSGFKNNAPQFNFVCR 428
                   S+ LVDLVDV+LGKEYELV+TTY+GLYRYR+GDILRVSGFKN APQFNFVCR
Sbjct: 361 D----RKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCR 420

Query: 429 KNIILSIDMDKVEEVELQNAVMEGSKNLIPYGGFVHEYTSYPDIHTIPGHYVIYWEISLK 488
           KN+ILSID DKV+EVELQNAVME SKNLIPY   + EYTSY D HT+PGHYVIYWEIS K
Sbjct: 421 KNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQK 480

Query: 489 ETTTIPPSVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIVENGTFDKLMDYAINHGS 548
           ET  IP  VFE CCLT+EESLN +YR+GRVS KSIGPLEIKI+ENGTFDKLMDYAINHGS
Sbjct: 481 ETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGS 540

Query: 549 SINQYKTPRCVTKAPILELLNSCVVSNHFSPKCPKWIPGPKEWSTE 592
           SINQYKTPRCV   PILELLNS VVSN+FSP+CPKW+PG KEWST+
Sbjct: 541 SINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPKWVPGKKEWSTK 581

BLAST of Bhi10G000937 vs. NCBI nr
Match: XP_023533206.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 948.0 bits (2449), Expect = 4.0e-272
Identity = 459/586 (78.33%), Postives = 514/586 (87.71%), Query Frame = 0

Query: 9   LTSKDEQSILQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGCTDRKTFKNTIP 68
           +  +DE +ILQHIEE+TTHADQVQ RVLTEILSRNA VEYL++ GL+GC DR  FKN +P
Sbjct: 8   MPERDEGAILQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 67

Query: 69  LVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTEEEQKRRSLLYF 128
           +VSYE+LKPYIDRIANGDASPILCS+P+SEF  SSGTSGGE KL+PTTEEE KRRS LY 
Sbjct: 68  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 127

Query: 129 LLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHFKNRP---YTDY 188
           LLMPV+NQF+PGL++GKGMYFLFVKSES TPGGL ARP LTS+ KS HFK+RP   YTDY
Sbjct: 128 LLMPVMNQFIPGLNRGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 187

Query: 189 TSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQTHWPLLVNDIR 248
           TSP +TILC+DSYQSMYSQ LCGLCLS+QVLRVGSVFAS FIRAIKFL+THW LL NDIR
Sbjct: 188 TSPNQTILCTDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 247

Query: 249 TGTINNSKITDPLVRESVMNILKPDPLLADFIEFECVKESWGGIIIRLWPNTKYVDVIVT 308
           TGTI N +I+DPLVR+SVMNILKPDP+LAD IEFEC K +W GII RLWPNTKY+DVIVT
Sbjct: 248 TGTI-NPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVT 307

Query: 309 GTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLIPSMAYFEFLPT 368
           GTMSQYIPTLDYY+N LP+V +MYASSEC  G+NLNPLCNPN+VSYTLIP+MAYFEFLP 
Sbjct: 308 GTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPL 367

Query: 369 TAHGFSNSSQLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDILRVSGFKNNAPQFNFVCR 428
                   S+ LVDLVDV+LGKEYELV+TTY+GLYRYR+GDILRVSGFKN APQFNFVCR
Sbjct: 368 D----REMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCR 427

Query: 429 KNIILSIDMDKVEEVELQNAVMEGSKNLIPYGGFVHEYTSYPDIHTIPGHYVIYWEISLK 488
           KN+ILSID DKV+EVELQNAVME SKNL+PY   + EYTSY D HT+PGHYVIYWEIS K
Sbjct: 428 KNVILSIDSDKVDEVELQNAVMEASKNLVPYETSLLEYTSYADTHTVPGHYVIYWEISQK 487

Query: 489 ETTTIPPSVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIVENGTFDKLMDYAINHGS 548
           ET  IP  VFE CCLT+EESLN +YR+GRVS KSIGPLE+KI+ENGTFDKLMDYAINHGS
Sbjct: 488 ETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLELKIIENGTFDKLMDYAINHGS 547

Query: 549 SINQYKTPRCVTKAPILELLNSCVVSNHFSPKCPKWIPGPKEWSTE 592
           SINQYKTPRCV   PILELLNS VVSN+FSP+CPKW+PG KEWS++
Sbjct: 548 SINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWSSK 588

BLAST of Bhi10G000937 vs. ExPASy TrEMBL
Match: A0A6J1I4E6 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=LOC111469778 PE=3 SV=1)

HSP 1 Score: 949.5 bits (2453), Expect = 6.6e-273
Identity = 462/586 (78.84%), Postives = 514/586 (87.71%), Query Frame = 0

Query: 9   LTSKDEQSILQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGCTDRKTFKNTIP 68
           +  KDE ++LQHIEE+TTHADQVQ RVLTEILSRNA VEYL++ GL+GC DR  FKN +P
Sbjct: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60

Query: 69  LVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTEEEQKRRSLLYF 128
           +VSYE+LKPYIDRIANGDASPILCS+PISEF  SSGTSGGE KL+PTT+EE KRRS LY 
Sbjct: 61  VVSYENLKPYIDRIANGDASPILCSEPISEFLTSSGTSGGERKLMPTTKEELKRRSFLYS 120

Query: 129 LLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHFKNRP---YTDY 188
           LLMPV+NQF+PGL+KGKGMYFLFVKSES TPGGL ARP LTS+ KS HFK+RP   YTDY
Sbjct: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180

Query: 189 TSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQTHWPLLVNDIR 248
           TSP +TILC+DSYQSMYSQ LCGLCLS+QVLRVGSVFAS FIRAIKFL+THW LL NDIR
Sbjct: 181 TSPNQTILCTDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240

Query: 249 TGTINNSKITDPLVRESVMNILKPDPLLADFIEFECVKESWGGIIIRLWPNTKYVDVIVT 308
           TGTI N +I+DPLVR+SVMNILKPDP+LAD IEFEC K +W GII RLWPNTKYVDVIVT
Sbjct: 241 TGTI-NPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIITRLWPNTKYVDVIVT 300

Query: 309 GTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLIPSMAYFEFLPT 368
           GTMSQYIPTLD Y+N LP+V +MYASSEC  G+NLNPLCNPN+VSYTLIP+MAYFEFLP 
Sbjct: 301 GTMSQYIPTLDKYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPL 360

Query: 369 TAHGFSNSSQLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDILRVSGFKNNAPQFNFVCR 428
                   S+ LVDLVDV+LGKEYEL++TTY+GLYRYR+GDILRVSGFKN APQFNFVCR
Sbjct: 361 D----RKMSEELVDLVDVELGKEYELIITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCR 420

Query: 429 KNIILSIDMDKVEEVELQNAVMEGSKNLIPYGGFVHEYTSYPDIHTIPGHYVIYWEISLK 488
           KN+ILSID DKV+EVELQNAVME SKNLIPY   + EYTSY D HT+PGHYVIYWEIS K
Sbjct: 421 KNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQK 480

Query: 489 ETTTIPPSVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIVENGTFDKLMDYAINHGS 548
           ET  IP  VFE CCLT+EESLN +YR+GRVS KSIGPLE+KI+ENGTFDKLMDYAINHGS
Sbjct: 481 ETNAIPDWVFENCCLTIEESLNSVYRQGRVSDKSIGPLELKIIENGTFDKLMDYAINHGS 540

Query: 549 SINQYKTPRCVTKAPILELLNSCVVSNHFSPKCPKWIPGPKEWSTE 592
           SINQYKTPRCV + PILELLNS VVSN+FSPKCPKW+PG KEWST+
Sbjct: 541 SINQYKTPRCVKREPILELLNSYVVSNYFSPKCPKWVPGQKEWSTK 581

BLAST of Bhi10G000937 vs. ExPASy TrEMBL
Match: A0A6J1G8Z2 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111451824 PE=3 SV=1)

HSP 1 Score: 949.5 bits (2453), Expect = 6.6e-273
Identity = 462/586 (78.84%), Postives = 512/586 (87.37%), Query Frame = 0

Query: 9   LTSKDEQSILQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGCTDRKTFKNTIP 68
           +  KDE ++LQHIEE+TTHADQVQ RVLTEILSRNA VEYL++ GL+GC DR  FKN +P
Sbjct: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60

Query: 69  LVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTEEEQKRRSLLYF 128
           +VSYE+LKPYIDRIANGDASPILCS+P+SEF  SSGTSGGE KL+PTTEEE KRRS LY 
Sbjct: 61  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 120

Query: 129 LLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHFKNRP---YTDY 188
           LLMPV+NQF+PGL+KGKGMYFLFVKSES TPGGL ARP LTS+ KS HFK+RP   YTDY
Sbjct: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180

Query: 189 TSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQTHWPLLVNDIR 248
           TSP +TILC+DSYQSMY Q LCGLCLS+QVLRVGSVFAS FIRAIKFL+THW LL NDIR
Sbjct: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240

Query: 249 TGTINNSKITDPLVRESVMNILKPDPLLADFIEFECVKESWGGIIIRLWPNTKYVDVIVT 308
           TGTI N +I DPLVR+SVMNILKPDP+LAD IEFEC K +W GII RLWPNTKY+DVIVT
Sbjct: 241 TGTI-NPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVT 300

Query: 309 GTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLIPSMAYFEFLPT 368
           GTMSQYIPTLDYY+N LP+V +MYASSEC  G+NLNPLCNPN+VSYTLIP+MAYFEFLP 
Sbjct: 301 GTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPL 360

Query: 369 TAHGFSNSSQLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDILRVSGFKNNAPQFNFVCR 428
                   S+ LVDLVDV+LGKEYELV+TTY+GLYRYR+GDILRVSGFKN APQFNFVCR
Sbjct: 361 D----RKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCR 420

Query: 429 KNIILSIDMDKVEEVELQNAVMEGSKNLIPYGGFVHEYTSYPDIHTIPGHYVIYWEISLK 488
           KN+ILSID DKV+EVELQNAVME SKNLIPY   + EYTSY D HT+PGHYVIYWEIS K
Sbjct: 421 KNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQK 480

Query: 489 ETTTIPPSVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIVENGTFDKLMDYAINHGS 548
           ET  IP  VFE CCLT+EESLN +YR+GRVS KSIGPLEIKI+ENGTFDKLMDYAINHGS
Sbjct: 481 ETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGS 540

Query: 549 SINQYKTPRCVTKAPILELLNSCVVSNHFSPKCPKWIPGPKEWSTE 592
           SINQYKTPRCV   PILELLNS VVSN+FSP+CPKW+PG KEWST+
Sbjct: 541 SINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWSTK 581

BLAST of Bhi10G000937 vs. ExPASy TrEMBL
Match: A0A6J1CG47 (indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011212 PE=3 SV=1)

HSP 1 Score: 924.9 bits (2389), Expect = 1.7e-265
Identity = 467/619 (75.44%), Postives = 518/619 (83.68%), Query Frame = 0

Query: 4   EVSNNLTSKDEQSI----------LQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHG 63
           E SNNL  + +  I          LQ+IE+VT HADQVQ RVLTEILS NA VEYL RHG
Sbjct: 3   EASNNLPPEPDSLIGSSHEKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYL-RHG 62

Query: 64  LNGCTDRKTFKNTIPLVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLI 123
           L+G  D KTFK T+P+VSY+D+KPY+DRIANGD SPILCS+PISEF  SSGTSGGE KL+
Sbjct: 63  LDGYMDTKTFKKTMPVVSYDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKLM 122

Query: 124 PTTEEEQKRRSLLYFLLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCK 183
           PTTEEE +R+SLLY LLMPV+NQF+PGLDKGKGMYFLFVKSES TPGGLLARP LTS+ +
Sbjct: 123 PTTEEELERKSLLYSLLMPVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYR 182

Query: 184 SPHFKNR---PYTDYTSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAI 243
           SPHFK+R   PY  YTSP ETILC+DSYQSMYSQ LCGLCLS++VLRVG+VFAS FIRAI
Sbjct: 183 SPHFKDRPYDPYNQYTSPNETILCTDSYQSMYSQFLCGLCLSREVLRVGAVFASGFIRAI 242

Query: 244 KFLQTHWPLLVNDIRTGTINNSKITDPLVRESVMNILKPDPLLADFIEFECVKESWGGII 303
           KFL+THW LL NDIRTGTI N +ITDPLVR+SV+NILKPDP LAD IE EC K +W GII
Sbjct: 243 KFLETHWLLLSNDIRTGTI-NPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGII 302

Query: 304 IRLWPNTKYVDVIVTGTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVS 363
            RLW NTKY+DVIVTGTMSQYIPTLDYY+N LP+V +MYASSEC  GINLNPLCNP++VS
Sbjct: 303 SRLWRNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVS 362

Query: 364 YTLIPSMAYFEFLP---TTAHGFSN---------SSQLLVDLVDVQLGKEYELVVTTYAG 423
           YTLIP+MAYFEFLP   T   G+S+         + Q LVDLVDVQLGKEYELV+TTYAG
Sbjct: 363 YTLIPTMAYFEFLPLDGTNGFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITTYAG 422

Query: 424 LYRYRMGDILRVSGFKNNAPQFNFVCRKNIILSIDMDKVEEVELQNAVMEGSKNLIPYGG 483
           LYRYR+GDILRVSGFKNNAPQFNFVCRKN+ILSID DKV+EVELQNAVME SKNLIPYG 
Sbjct: 423 LYRYRVGDILRVSGFKNNAPQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYGA 482

Query: 484 FVHEYTSYPDIHTIPGHYVIYWEISLKETT-----TIPPSVFEQCCLTMEESLNVMYREG 543
            V EYTSY D  TIPGHYVIYWEISLKE T      IP SVFE+CCL+MEESLN +YR+G
Sbjct: 483 CVVEYTSYADTVTIPGHYVIYWEISLKEATANNNNVIPGSVFEKCCLSMEESLNSVYRQG 542

Query: 544 RVSVKSIGPLEIKIVENGTFDKLMDYAINHGSSINQYKTPRCVTKAPILELLNSCVVSNH 593
           RVS KSIGPLE+KIVE GTFDKLMDYA+NHG+SINQYKTPRCV  APILELLNS VV N+
Sbjct: 543 RVSDKSIGPLEMKIVETGTFDKLMDYALNHGASINQYKTPRCVKHAPILELLNSYVVDNY 602

BLAST of Bhi10G000937 vs. ExPASy TrEMBL
Match: A0A1S4CBP6 (indole-3-acetic acid-amido synthetase GH3.6 OS=Nicotiana tabacum OX=4097 GN=LOC107817256 PE=3 SV=1)

HSP 1 Score: 871.3 bits (2250), Expect = 2.3e-249
Identity = 425/600 (70.83%), Postives = 502/600 (83.67%), Query Frame = 0

Query: 8   NLTSKDEQSILQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGCTDRKTFKNTI 67
           ++ +++ + +LQ IEEVTT+AD+VQ RVL EILS+NA VEYL RH LNG TDR TFK T+
Sbjct: 11  SVVAENNKKVLQFIEEVTTNADEVQKRVLAEILSQNAHVEYLHRHDLNGHTDRDTFKKTM 70

Query: 68  PLVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTEEEQKRRSLLY 127
           P+++YED++  I RIANGD SPILCSQPISEF  SSGTSGGE KL+PT EEE +RRS LY
Sbjct: 71  PVITYEDIQSVITRIANGDKSPILCSQPISEFLTSSGTSGGERKLMPTIEEELERRSKLY 130

Query: 128 FLLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHFKNR---PYTD 187
            LLMPV++QFVP L+KGKGMYFLF+KSE+ TPGGL ARP LTS+ KSPHFKNR   PYT+
Sbjct: 131 SLLMPVMSQFVPDLEKGKGMYFLFIKSEAKTPGGLPARPVLTSYYKSPHFKNRRPDPYTN 190

Query: 188 YTSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQTHWPLLVNDI 247
           YTSP ETILCSDSYQSMYSQ+LCGLC +K+VLRVG+VFAS FIRAI+FL+ HWPLL +DI
Sbjct: 191 YTSPNETILCSDSYQSMYSQMLCGLCQNKEVLRVGAVFASGFIRAIRFLEKHWPLLCHDI 250

Query: 248 RTGTINNSKITDPLVRESVMNILKPDPLLADFIEFECVKESWGGIIIRLWPNTKYVDVIV 307
           RTGTI NS+ITDP VRE+VM ILKPD  LADFIE EC K SW GII RLWPNTKY+DVIV
Sbjct: 251 RTGTI-NSQITDPSVREAVMKILKPDNKLADFIEVECSKNSWQGIITRLWPNTKYIDVIV 310

Query: 308 TGTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLIPSMAYFEFLP 367
           TGTMSQYIPTLDYY+N LP+V +MYASSEC  G+NLNPLC P++V+YTLIP+M YFEFLP
Sbjct: 311 TGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPTMGYFEFLP 370

Query: 368 T-TAHGFSNS-----------SQLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDILRVSG 427
               +G +NS            Q LVDLVDV++G+EYELVVTTYAGLYRYR+GD+LRV+G
Sbjct: 371 VHRNNGVTNSISMPKSLNEKEQQELVDLVDVKIGQEYELVVTTYAGLYRYRVGDVLRVAG 430

Query: 428 FKNNAPQFNFVCRKNIILSIDMDKVEEVELQNAVMEGSKNLIPYGGFVHEYTSYPDIHTI 487
           FKNNAPQFNF+CRKN++LSID DK +EVELQNAV     +L+P+   V EYTSY D  TI
Sbjct: 431 FKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNAVSHLMPFDAHVTEYTSYADTTTI 490

Query: 488 PGHYVIYWEISLKETTTIPPSVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIVENGT 547
           PGHYV++WE+++  +T +PPSVFE CCLT+EESLN +YR+GR S KSIGPLEIKIVE+GT
Sbjct: 491 PGHYVLFWELNVNGSTPVPPSVFEDCCLTIEESLNSVYRQGRASDKSIGPLEIKIVESGT 550

Query: 548 FDKLMDYAINHGSSINQYKTPRCVTKAPILELLNSCVVSNHFSPKCPKWIPGPKEWSTEN 593
           FDKLMDYAI+ G+SINQYKTPRCV  API+ELLNS VVS++FSPKCPKW+PG K+W+  N
Sbjct: 551 FDKLMDYAISLGASINQYKTPRCVKFAPIVELLNSRVVSSYFSPKCPKWVPGLKQWNNMN 609

BLAST of Bhi10G000937 vs. ExPASy TrEMBL
Match: A0A1U7VNZ1 (indole-3-acetic acid-amido synthetase GH3.6 OS=Nicotiana sylvestris OX=4096 GN=LOC104215495 PE=3 SV=1)

HSP 1 Score: 871.3 bits (2250), Expect = 2.3e-249
Identity = 425/600 (70.83%), Postives = 502/600 (83.67%), Query Frame = 0

Query: 8   NLTSKDEQSILQHIEEVTTHADQVQGRVLTEILSRNADVEYLKRHGLNGCTDRKTFKNTI 67
           ++ +++ + +LQ IEEVTT+AD+VQ RVL EILS+NA VEYL RH LNG TDR TFK T+
Sbjct: 11  SVVAENNKKVLQFIEEVTTNADEVQKRVLAEILSQNAHVEYLHRHDLNGHTDRDTFKKTM 70

Query: 68  PLVSYEDLKPYIDRIANGDASPILCSQPISEFFISSGTSGGESKLIPTTEEEQKRRSLLY 127
           P+++YED++  I RIANGD SPILCSQPISEF  SSGTSGGE KL+PT EEE +RRS LY
Sbjct: 71  PVITYEDIQSVITRIANGDKSPILCSQPISEFLTSSGTSGGERKLMPTIEEELERRSKLY 130

Query: 128 FLLMPVINQFVPGLDKGKGMYFLFVKSESITPGGLLARPALTSFCKSPHFKNR---PYTD 187
            LLMPV++QFVP L+KGKGMYFLF+KSE+ TPGGL ARP LTS+ KSPHFKNR   PYT+
Sbjct: 131 SLLMPVMSQFVPDLEKGKGMYFLFIKSEAKTPGGLPARPVLTSYYKSPHFKNRRPDPYTN 190

Query: 188 YTSPIETILCSDSYQSMYSQLLCGLCLSKQVLRVGSVFASAFIRAIKFLQTHWPLLVNDI 247
           YTSP ETILCSDSYQSMYSQ+LCGLC +K+VLRVG+VFAS FIRAI+FL+ HWPLL +DI
Sbjct: 191 YTSPNETILCSDSYQSMYSQMLCGLCQNKEVLRVGAVFASGFIRAIRFLEKHWPLLCHDI 250

Query: 248 RTGTINNSKITDPLVRESVMNILKPDPLLADFIEFECVKESWGGIIIRLWPNTKYVDVIV 307
           RTGTI NS+ITDP VRE+VM ILKPD  LADFIE EC K SW GII RLWPNTKY+DVIV
Sbjct: 251 RTGTI-NSQITDPSVREAVMKILKPDNKLADFIEVECSKNSWQGIITRLWPNTKYIDVIV 310

Query: 308 TGTMSQYIPTLDYYTNSLPIVSSMYASSECNSGINLNPLCNPNQVSYTLIPSMAYFEFLP 367
           TGTMSQYIPTLDYY+N LP+V +MYASSEC  G+NLNPLC P++V+YTLIP+M YFEFLP
Sbjct: 311 TGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPTMGYFEFLP 370

Query: 368 T-TAHGFSNS-----------SQLLVDLVDVQLGKEYELVVTTYAGLYRYRMGDILRVSG 427
               +G +NS            Q LVDLVDV++G+EYELVVTTYAGLYRYR+GD+LRV+G
Sbjct: 371 VHRNNGVTNSISMPKSLNEKEQQELVDLVDVKIGQEYELVVTTYAGLYRYRVGDVLRVAG 430

Query: 428 FKNNAPQFNFVCRKNIILSIDMDKVEEVELQNAVMEGSKNLIPYGGFVHEYTSYPDIHTI 487
           FKNNAPQFNF+CRKN++LSID DK +EVELQNAV     +L+P+   V EYTSY D  TI
Sbjct: 431 FKNNAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNAVSHLMPFDAHVTEYTSYADTTTI 490

Query: 488 PGHYVIYWEISLKETTTIPPSVFEQCCLTMEESLNVMYREGRVSVKSIGPLEIKIVENGT 547
           PGHYV++WE+++  +T +PPSVFE CCLT+EESLN +YR+GR S KSIGPLEIKIVE+GT
Sbjct: 491 PGHYVLFWELNVNGSTPVPPSVFEDCCLTIEESLNSVYRQGRASDKSIGPLEIKIVESGT 550

Query: 548 FDKLMDYAINHGSSINQYKTPRCVTKAPILELLNSCVVSNHFSPKCPKWIPGPKEWSTEN 593
           FDKLMDYAI+ G+SINQYKTPRCV  API+ELLNS VVS++FSPKCPKW+PG K+W+  N
Sbjct: 551 FDKLMDYAISLGASINQYKTPRCVKFAPIVELLNSRVVSSYFSPKCPKWVPGLKQWNNMN 609