Bhi10G000356 (gene) Wax gourd (B227) v1

Overview
NameBhi10G000356
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionAlpha/beta-Hydrolases superfamily protein
Locationchr10: 9740952 .. 9743328 (+)
RNA-Seq ExpressionBhi10G000356
SyntenyBhi10G000356
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTCTCTTCCTTTTTTACCTGTTCTTATTTCTGTGACTGGAGCTACATAGATTTTATTTTGCTTTGGTTAGACGGATGGTATGGATCATGAATAGTTGATATTTTTGTACGGATGTTTTGCCAAAATAATAATAATAATAAAATAATGAATAAAAACAGAAGCTTGTACAAAAAAATGTGATCCGGCCTATTTTTGGCTGTTGTGCGGGTGGTTTTTTGCGTCTGCAAATTGGTATTTTTTGAAGCAGGCACCATATAATGTCTGCCTCGTCATTTTTCATGTGTAGGTTTTCGTCTTCCTTCACATTAGAATTATTCACACAACAAATAACACTAGTTTGATAAAATATAGATAATAAACAAAAAACAAAACAAAAAACAAAGATGGTATCCGATAGCACAGCAGTACCCACCAGAAAACGGCAGACTTTGGCGGCTGCCGGCCACTCGCCATTATCTCTCCTTTAATTCTTCCCTTCTTCCACCTTTCCTTCTCATCTCATCTTTTCTCTTCTCTTCTGTATTGACTTGAAGCCATGGCTACCACTCCCTTTGAAGTCGATGAGTGCCGTGGTGTCCTCCGCGTTTACAGCGATGGCTCCATAGTCCGTTCCTCCAATCCGAGCTTCAATGTCCCTGTCTTCGACGATGGTTCCGTTCTATGGAAAGATCTTCTCTTTGATCCCATCCACGATCTTCACCTCCGTCTCTACAAACCTGCTTCCATTTCTTCTTCCAAGCTTCCCGTCCTTTTCTACATTCACGGCGGCGGCTTCTGCATCGGCTCCCGCACCTGGCCCAATTGCCAGAACTACTGCTTCCGCCTCGCCGCCGAGCTTCCTGCTCTCGTCATCTCCCCGGACTATCGTCTCGCTCCCGAGAATCGCCTTCCGGCCGCTATCGATGACGGCTTCGCGGCTCTCCGGTGGCTCCAAGCCCAAGCTGAATCCGACCATCCTGATCCCTGGCTGGCCGACGTCGCCGATTTCAGCAGAGTTTTCATCAGCGGTGACTCCGCCGGTGGGAACATCGCTCACCATCTTGCGGCGGGACTGGGCCTAGGTTCGCCTGAGTTGGCGCCAGTTCGAGTGAGAGGCTACGTTCTCCTGGCGCCATTCTTCGGCGGGACGGTGAGGACTCGGTCCGAGGCAGAAGGCTCCAAAGAAGCCTTCCTGAATCTGGAGCTCATCGATAGGTTGAAATCAGATTGTGAAATTTGAATTTGAAGTTCAATTTGAGTTACAATTTCAGTGAAGTTAAGATTTGGTTTTAACCATTTGAGCAGGTTTTGGAGATTGTCGATCCCGATCGGAAGCAGCGCCGATCATCCTCTTGTGGACGTGTTTGGACCGAGCAGCCGGAACTTGAAGGCGGTGGAGATGGATCCGATAATGGTGGTGGTGGGCGGCGCTGATCTGTTGAAAGATCGAGCGGTGGAGTACGCAGAGAAGCTGAAGAAACAGGGGAAGAAGATAGAATTGGTGGAGTTTGAAGGAAAACAACACGGATTCTTCACCATTGATCCAAATTCTGAAGCTTCTAACCAATTAATGCTTCTCATCAACCACTTCATTGCTCAACATTCACTCTGATTCCTTCATCCCTTTTCCATTCCCCTATCAATCTGCTTAATAAAAATGTTCCAATCGTAATCCATATGTTCATAAACCATCAAACTATGTTTTAATTTTCTTTTAAAAACAAACAAACACAATTTCAAGATAAATTAGAGCTCCAGGACATATGGATCATGGTGAGAGTGATCGAGGAACAGAAGGCCATATGGATCATGGTGAGAGAGATCGAGGAAACGTCTATTGATAATTGAAGTTGATTAATTAATTAAAGGGTCATTTTTAAATATAGGAAAATGAACCAAAAAGTGGATAATTCATCATCATGAATTAAAAAAAAAAAAAATGAAACTTTGAACAGTGGTGGAGTAGGCTTCGGAAATTGAAACTTCCTAACAAGATTCAAAATTTTATGTGAATAAAATTCACAACATTTTACCATCAAACCCCAATCTAAAGAGACGAATGCTCCTTATTTAGGTGTGTGTTGTGAAAAAGATTGAAATTCAATTGAACATGTCTTTTTTCAAGCGGGCAAAGCTGTTGTGGCAATTCACAATGTTTGTAGGAACTTCGCTCGTTGTTGGAGTAGTAGATGACACACAATCTAAGCTTGTTGGCCTTTGTCCAATCGAATGCATGATGTTTGGGTCGAAAATTTTTCTTTATCATTTGTGCCTTCGGGTTGATGTTGATGGTTGCAGCCCTTCAAGCATTTTGATTTCTTTACGTTATAGCTTCATTTAAAAAAAAATGTGAAATATTGATTATGTATTTTTTTTCTTTTTTCAGTACGATGGGA

mRNA sequence

TTCTCTCTTCCTTTTTTACCTGTTCTTATTTCTGTGACTGGAGCTACATAGATTTTATTTTGCTTTGGTTAGACGGATGGTATGGATCATGAATAGTTGATATTTTTGTACGGATGTTTTGCCAAAATAATAATAATAATAAAATAATGAATAAAAACAGAAGCTTGTACAAAAAAATGTGATCCGGCCTATTTTTGGCTGTTGTGCGGGTGGTTTTTTGCGTCTGCAAATTGGTATTTTTTGAAGCAGGCACCATATAATGTCTGCCTCGTCATTTTTCATGTGTAGGTTTTCGTCTTCCTTCACATTAGAATTATTCACACAACAAATAACACTAGTTTGATAAAATATAGATAATAAACAAAAAACAAAACAAAAAACAAAGATGGTATCCGATAGCACAGCAGTACCCACCAGAAAACGGCAGACTTTGGCGGCTGCCGGCCACTCGCCATTATCTCTCCTTTAATTCTTCCCTTCTTCCACCTTTCCTTCTCATCTCATCTTTTCTCTTCTCTTCTGTATTGACTTGAAGCCATGGCTACCACTCCCTTTGAAGTCGATGAGTGCCGTGGTGTCCTCCGCGTTTACAGCGATGGCTCCATAGTCCGTTCCTCCAATCCGAGCTTCAATGTCCCTGTCTTCGACGATGGTTCCGTTCTATGGAAAGATCTTCTCTTTGATCCCATCCACGATCTTCACCTCCGTCTCTACAAACCTGCTTCCATTTCTTCTTCCAAGCTTCCCGTCCTTTTCTACATTCACGGCGGCGGCTTCTGCATCGGCTCCCGCACCTGGCCCAATTGCCAGAACTACTGCTTCCGCCTCGCCGCCGAGCTTCCTGCTCTCGTCATCTCCCCGGACTATCGTCTCGCTCCCGAGAATCGCCTTCCGGCCGCTATCGATGACGGCTTCGCGGCTCTCCGGTGGCTCCAAGCCCAAGCTGAATCCGACCATCCTGATCCCTGGCTGGCCGACGTCGCCGATTTCAGCAGAGTTTTCATCAGCGGTGACTCCGCCGGTGGGAACATCGCTCACCATCTTGCGGCGGGACTGGGCCTAGGTTCGCCTGAGTTGGCGCCAGTTCGAGTGAGAGGCTACGTTCTCCTGGCGCCATTCTTCGGCGGGACGGTGAGGACTCGGTCCGAGGCAGAAGGCTCCAAAGAAGCCTTCCTGAATCTGGAGCTCATCGATAGGTTTTGGAGATTGTCGATCCCGATCGGAAGCAGCGCCGATCATCCTCTTGTGGACGTGTTTGGACCGAGCAGCCGGAACTTGAAGGCGGTGGAGATGGATCCGATAATGGTGGTGGTGGGCGGCGCTGATCTGTTGAAAGATCGAGCGGTGGAGTACGCAGAGAAGCTGAAGAAACAGGGGAAGAAGATAGAATTGGTGGAGTTTGAAGGAAAACAACACGGATTCTTCACCATTGATCCAAATTCTGAAGCTTCTAACCAATTAATGCTTCTCATCAACCACTTCATTGCTCAACATTCACTCTGATTCCTTCATCCCTTTTCCATTCCCCTATCAATCTGCTTAATAAAAATGTTCCAATCGTAATCCATATGTTCATAAACCATCAAACTATGTTTTAATTTTCTTTTAAAAACAAACAAACACAATTTCAAGATAAATTAGAGCTCCAGGACATATGGATCATGGTGAGAGTGATCGAGGAACAGAAGGCCATATGGATCATGGTGAGAGAGATCGAGGAAACGTCTATTGATAATTGAAGTTGATTAATTAATTAAAGGGTCATTTTTAAATATAGGAAAATGAACCAAAAAGTGGATAATTCATCATCATGAATTAAAAAAAAAAAAAATGAAACTTTGAACAGTGGTGGAGTAGGCTTCGGAAATTGAAACTTCCTAACAAGATTCAAAATTTTATGTGAATAAAATTCACAACATTTTACCATCAAACCCCAATCTAAAGAGACGAATGCTCCTTATTTAGGTGTGTGTTGTGAAAAAGATTGAAATTCAATTGAACATGTCTTTTTTCAAGCGGGCAAAGCTGTTGTGGCAATTCACAATGTTTGTAGGAACTTCGCTCGTTGTTGGAGTAGTAGATGACACACAATCTAAGCTTGTTGGCCTTTGTCCAATCGAATGCATGATGTTTGGGTCGAAAATTTTTCTTTATCATTTGTGCCTTCGGGTTGATGTTGATGGTTGCAGCCCTTCAAGCATTTTGATTTCTTTACGTTATAGCTTCATTTAAAAAAAAATGTGAAATATTGATTATGTATTTTTTTTCTTTTTTCAGTACGATGGGA

Coding sequence (CDS)

ATGGCTACCACTCCCTTTGAAGTCGATGAGTGCCGTGGTGTCCTCCGCGTTTACAGCGATGGCTCCATAGTCCGTTCCTCCAATCCGAGCTTCAATGTCCCTGTCTTCGACGATGGTTCCGTTCTATGGAAAGATCTTCTCTTTGATCCCATCCACGATCTTCACCTCCGTCTCTACAAACCTGCTTCCATTTCTTCTTCCAAGCTTCCCGTCCTTTTCTACATTCACGGCGGCGGCTTCTGCATCGGCTCCCGCACCTGGCCCAATTGCCAGAACTACTGCTTCCGCCTCGCCGCCGAGCTTCCTGCTCTCGTCATCTCCCCGGACTATCGTCTCGCTCCCGAGAATCGCCTTCCGGCCGCTATCGATGACGGCTTCGCGGCTCTCCGGTGGCTCCAAGCCCAAGCTGAATCCGACCATCCTGATCCCTGGCTGGCCGACGTCGCCGATTTCAGCAGAGTTTTCATCAGCGGTGACTCCGCCGGTGGGAACATCGCTCACCATCTTGCGGCGGGACTGGGCCTAGGTTCGCCTGAGTTGGCGCCAGTTCGAGTGAGAGGCTACGTTCTCCTGGCGCCATTCTTCGGCGGGACGGTGAGGACTCGGTCCGAGGCAGAAGGCTCCAAAGAAGCCTTCCTGAATCTGGAGCTCATCGATAGGTTTTGGAGATTGTCGATCCCGATCGGAAGCAGCGCCGATCATCCTCTTGTGGACGTGTTTGGACCGAGCAGCCGGAACTTGAAGGCGGTGGAGATGGATCCGATAATGGTGGTGGTGGGCGGCGCTGATCTGTTGAAAGATCGAGCGGTGGAGTACGCAGAGAAGCTGAAGAAACAGGGGAAGAAGATAGAATTGGTGGAGTTTGAAGGAAAACAACACGGATTCTTCACCATTGATCCAAATTCTGAAGCTTCTAACCAATTAATGCTTCTCATCAACCACTTCATTGCTCAACATTCACTCTGA

Protein sequence

MATTPFEVDECRGVLRVYSDGSIVRSSNPSFNVPVFDDGSVLWKDLLFDPIHDLHLRLYKPASISSSKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLPAAIDDGFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAAGLGLGSPELAPVRVRGYVLLAPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSSADHPLVDVFGPSSRNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQGKKIELVEFEGKQHGFFTIDPNSEASNQLMLLINHFIAQHSL
Homology
BLAST of Bhi10G000356 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 337.0 bits (863), Expect = 1.6e-92
Identity = 163/319 (51.10%), Postives = 231/319 (72.41%), Query Frame = 0

Query: 5   PFEVDECRGVLRVYSDGSIVRSSNPSF---NVPVFDDGSVLWKDLLFDPIHDLHLRLYKP 64
           P   ++C G+L++ S+G+++RS +       +P  ++ +VL+KD ++   ++LHLRLYKP
Sbjct: 8   PQVAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKP 67

Query: 65  ASISS-SKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLPA 124
            S S+ + LPV+ + HGGGFC GSR+WP+  N+C  LA+ L ALV+SPDYRLAPE+RLPA
Sbjct: 68  ISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPA 127

Query: 125 AIDDGFAALRWLQAQAESDHPDPWLAD--VADFSRVFISGDSAGGNIAHHLAAGLGLGSP 184
           A +D  A L WL  QA SD  + W  D    DF RVF+ GDS+GGNIAH LA   G GS 
Sbjct: 128 AFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFGSGSI 187

Query: 185 ELAPVRVRGYVLLAPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSSADHPLVD 244
           EL PVRVRGYVL+ PFFGG  RT SE  G  EA L+L+L+D+FWRLS+P G++ DH + +
Sbjct: 188 ELTPVRVRGYVLMGPFFGGEERTNSE-NGPSEALLSLDLLDKFWRLSLPNGATRDHHMAN 247

Query: 245 VFGPSSRNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQ-GKKIELVEFEGKQHGFFT 304
            FGP+S  L+++ ++P++V+VGG++LL+DRA EYA KLKK  GK+++ +EFE K+HGF++
Sbjct: 248 PFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGFYS 307

Query: 305 IDPNSEASNQLMLLINHFI 317
             P+SEA+ Q++ +I  F+
Sbjct: 308 NYPSSEAAEQVLRIIGDFM 325

BLAST of Bhi10G000356 vs. TAIR 10
Match: AT5G06570.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 337.0 bits (863), Expect = 1.6e-92
Identity = 163/319 (51.10%), Postives = 231/319 (72.41%), Query Frame = 0

Query: 5   PFEVDECRGVLRVYSDGSIVRSSNPSF---NVPVFDDGSVLWKDLLFDPIHDLHLRLYKP 64
           P   ++C G+L++ S+G+++RS +       +P  ++ +VL+KD ++   ++LHLRLYKP
Sbjct: 8   PQVAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKP 67

Query: 65  ASISS-SKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLPA 124
            S S+ + LPV+ + HGGGFC GSR+WP+  N+C  LA+ L ALV+SPDYRLAPE+RLPA
Sbjct: 68  ISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPA 127

Query: 125 AIDDGFAALRWLQAQAESDHPDPWLAD--VADFSRVFISGDSAGGNIAHHLAAGLGLGSP 184
           A +D  A L WL  QA SD  + W  D    DF RVF+ GDS+GGNIAH LA   G GS 
Sbjct: 128 AFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFGSGSI 187

Query: 185 ELAPVRVRGYVLLAPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSSADHPLVD 244
           EL PVRVRGYVL+ PFFGG  RT SE  G  EA L+L+L+D+FWRLS+P G++ DH + +
Sbjct: 188 ELTPVRVRGYVLMGPFFGGEERTNSE-NGPSEALLSLDLLDKFWRLSLPNGATRDHHMAN 247

Query: 245 VFGPSSRNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQ-GKKIELVEFEGKQHGFFT 304
            FGP+S  L+++ ++P++V+VGG++LL+DRA EYA KLKK  GK+++ +EFE K+HGF++
Sbjct: 248 PFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGFYS 307

Query: 305 IDPNSEASNQLMLLINHFI 317
             P+SEA+ Q++ +I  F+
Sbjct: 308 NYPSSEAAEQVLRIIGDFM 325

BLAST of Bhi10G000356 vs. TAIR 10
Match: AT1G68620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 183.3 bits (464), Expect = 3.0e-46
Identity = 109/277 (39.35%), Postives = 157/277 (56.68%), Query Frame = 0

Query: 8   VDECRGVLRVYSDGSIVRSS-----NPSFNVPVFDDGSVLWKDLLFDPIHDLHLRLYKPA 67
           VDE  G+++VY DG + RS      +PS  + +     V   D++ D + ++  RLY P 
Sbjct: 24  VDEVEGLIKVYKDGHVERSQLLPCVDPSLPLEL----GVTCSDVVIDKLTNVWARLYVPM 83

Query: 68  SI---SSSKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLP 127
           +    S SKLP++ Y HGGGFC+GS +W     +  RL+A    LV+S +YRLAPEN LP
Sbjct: 84  TTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPLP 143

Query: 128 AAIDDGFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAAGLGLGSPE 187
           AA +DG  A+ WL  +A +D  + W A   DF R+F++GDSAGGNIA  +AA   L SPE
Sbjct: 144 AAYEDGVNAILWLN-KARND--NLW-AKQCDFGRIFLAGDSAGGNIAQQVAA--RLASPE 203

Query: 188 LAPVRVRGYVLLAPFFGGTVRTRSEAE--GSKEAFLNLELIDRFWRLSIPIGSSADHPLV 247
              +++ G +L+ PF+ G  RT SE      K A L L   D +WR+S+P G++ +HP  
Sbjct: 204 DLALKIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLPRGANREHPYC 263

Query: 248 DVFGPSSRNLKAVEMDPIMVVVGGADLLKDRAVEYAE 275
               P    +K+  +   +V V   DLL D  +E  +
Sbjct: 264 K---PVKMIIKSSTVTRTLVCVAEMDLLMDSNMEMCD 287

BLAST of Bhi10G000356 vs. TAIR 10
Match: AT5G16080.1 (carboxyesterase 17 )

HSP 1 Score: 180.3 bits (456), Expect = 2.5e-45
Identity = 109/322 (33.85%), Postives = 172/322 (53.42%), Query Frame = 0

Query: 8   VDECRGVLRVYSDGSIVRSSNPSFNVPVFDDGSVLWKDLLFD--PIHDLHLRLYKP---A 67
           V+E  G+++V++DG + R        P     S   K   FD    +D   R+Y P   A
Sbjct: 29  VEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSS---KATAFDIKLSNDTWTRVYIPDAAA 88

Query: 68  SISSSKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLPAAI 127
           +  S  LP+L Y HGGGFC+GS  W    ++   LA +   +++S +YRLAPE+RLPAA 
Sbjct: 89  ASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPEHRLPAAY 148

Query: 128 DDGFAALRWL---QAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAAGLGLGSPE 187
           DDG   + WL   Q      +P  WL+   + S VF++GDSAG NIA+ +A  +      
Sbjct: 149 DDGVNVVSWLVKQQISTGGGYPS-WLSK-CNLSNVFLAGDSAGANIAYQVAVRIMASGKY 208

Query: 188 LAPVRVRGYVLLAPFFGGTVRTRSEAE--GSKEAFLNLELIDRFWRLSIPIGSSADHPLV 247
              + ++G +L+ PFFGG  RT SE +   +K + L L   D +WRL++P G+S DHP  
Sbjct: 209 ANTLHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRLALPRGASRDHPWC 268

Query: 248 DVFGPSSRNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQGKKIELVEFEGKQHGFFT 307
           +    S+      ++   MV +   D+LK+R +E  + ++  GK++E +   G  H F  
Sbjct: 269 NPLMSSA----GAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGGVGHAFHI 328

Query: 308 IDPNS---EASNQLMLLINHFI 317
           +D +S   +  + +M  +++FI
Sbjct: 329 LDNSSVSRDRIHDMMCRLHNFI 341

BLAST of Bhi10G000356 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 172.6 bits (436), Expect = 5.3e-43
Identity = 107/273 (39.19%), Postives = 150/273 (54.95%), Query Frame = 0

Query: 45  DLLFDPIHDLHLRLYKPASISSSKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPAL 104
           D + D   DL  RLY P  +S  K+PV+ + HGGGF   S       N C R A +LPA 
Sbjct: 64  DFVVDQSRDLWFRLYTP-HVSGDKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAY 123

Query: 105 VISPDYRLAPENRLPAAIDDGFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGN 164
           VIS +YRLAPE+R PA  DDGF AL+++    E +H     A+ AD SR F +GDSAGGN
Sbjct: 124 VISVNYRLAPEHRYPAQYDDGFDALKYI----EENHGSILPAN-ADLSRCFFAGDSAGGN 183

Query: 165 IAHHLAAGL-GLGSPELAPVRVRGYVLLAPFFGGTVRTRSEAEGSKEAFLNLELIDRFWR 224
           IAH++A  +          V++ G + + PFFGG  RT +E +      ++ +  D  W+
Sbjct: 184 IAHNVAIRICREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWK 243

Query: 225 LSIPIGSSADHPLVDVFGPSSRNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQGKKI 284
               +G + DH  V+V GP++ ++  ++    MVVV G D LKD    Y E LK  GKK 
Sbjct: 244 ---AMGLNRDHEAVNVGGPNAVDISGLDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKA 303

Query: 285 ELVEFEGKQHGFFTIDPNSEASNQLMLLINHFI 317
            L+E+    H F+ I P    + QL++ I  F+
Sbjct: 304 TLIEYPNMFHAFY-IFPELPEAGQLIMRIKDFV 326

BLAST of Bhi10G000356 vs. ExPASy Swiss-Prot
Match: Q9FG13 (Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 2.3e-91
Identity = 163/319 (51.10%), Postives = 231/319 (72.41%), Query Frame = 0

Query: 5   PFEVDECRGVLRVYSDGSIVRSSNPSF---NVPVFDDGSVLWKDLLFDPIHDLHLRLYKP 64
           P   ++C G+L++ S+G+++RS +       +P  ++ +VL+KD ++   ++LHLRLYKP
Sbjct: 8   PQVAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKP 67

Query: 65  ASISS-SKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLPA 124
            S S+ + LPV+ + HGGGFC GSR+WP+  N+C  LA+ L ALV+SPDYRLAPE+RLPA
Sbjct: 68  ISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPA 127

Query: 125 AIDDGFAALRWLQAQAESDHPDPWLAD--VADFSRVFISGDSAGGNIAHHLAAGLGLGSP 184
           A +D  A L WL  QA SD  + W  D    DF RVF+ GDS+GGNIAH LA   G GS 
Sbjct: 128 AFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFGSGSI 187

Query: 185 ELAPVRVRGYVLLAPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSSADHPLVD 244
           EL PVRVRGYVL+ PFFGG  RT SE  G  EA L+L+L+D+FWRLS+P G++ DH + +
Sbjct: 188 ELTPVRVRGYVLMGPFFGGEERTNSE-NGPSEALLSLDLLDKFWRLSLPNGATRDHHMAN 247

Query: 245 VFGPSSRNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQ-GKKIELVEFEGKQHGFFT 304
            FGP+S  L+++ ++P++V+VGG++LL+DRA EYA KLKK  GK+++ +EFE K+HGF++
Sbjct: 248 PFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGFYS 307

Query: 305 IDPNSEASNQLMLLINHFI 317
             P+SEA+ Q++ +I  F+
Sbjct: 308 NYPSSEAAEQVLRIIGDFM 325

BLAST of Bhi10G000356 vs. ExPASy Swiss-Prot
Match: Q0ZPV7 (Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.3e-46
Identity = 113/321 (35.20%), Postives = 170/321 (52.96%), Query Frame = 0

Query: 5   PFEVDECRGVLRVYSDGSIVRSSNPSFNVPVFDDGSVLWKDLLFDPIHDLHLRLYKP--A 64
           P  ++  R + R     S   S +P+ + P      VL KDL  +P+H+  +RL+ P  A
Sbjct: 23  PIVLNPDRTITRPIQIPSTAASPDPTSSSP------VLTKDLALNPLHNTFVRLFLPRHA 82

Query: 65  SISSSKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLPAAI 124
             +S+KLP++ Y HGGGF + S       ++C  +A     ++ S DYRLAPE+RLPAA 
Sbjct: 83  LYNSAKLPLVVYFHGGGFILFSAASTIFHDFCCEMAVHAGVVIASVDYRLAPEHRLPAAY 142

Query: 125 DDGFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAAGLGLGSPELAP 184
           DD   AL+W++     D  D WL + ADFS  FI G+SAGGNIA+H        + EL P
Sbjct: 143 DDAMEALQWIK-----DSRDEWLTNFADFSNCFIMGESAGGNIAYHAGLRAAAVADELLP 202

Query: 185 VRVRGYVLLAPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSSADHPLVDVFGP 244
           ++++G VL  P FGG+ RT SE   + ++ L   ++D  W LS+P+G+  DH   +   P
Sbjct: 203 LKIKGLVLDEPGFGGSKRTGSELRLANDSRLPTFVLDLIWELSLPMGADRDH---EYCNP 262

Query: 245 SSRNLKAVEMDPI-------MVVVGGADLLKDRAVEYAEKLKKQGKKIELVEFEGKQHGF 304
           ++ +      D I       MVV    D + DR +E AE+L+K+G  +      G  H  
Sbjct: 263 TAESEPLYSFDKIRSLGWRVMVVGCHGDPMIDRQMELAERLEKKGVDVVAQFDVGGYHAV 322

Query: 305 FTIDPNSEASNQLMLLINHFI 317
              DP  E + Q  +++  F+
Sbjct: 323 KLEDP--EKAKQFFVILKKFV 327

BLAST of Bhi10G000356 vs. ExPASy Swiss-Prot
Match: Q9SX25 (Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.2e-45
Identity = 109/277 (39.35%), Postives = 157/277 (56.68%), Query Frame = 0

Query: 8   VDECRGVLRVYSDGSIVRSS-----NPSFNVPVFDDGSVLWKDLLFDPIHDLHLRLYKPA 67
           VDE  G+++VY DG + RS      +PS  + +     V   D++ D + ++  RLY P 
Sbjct: 24  VDEVEGLIKVYKDGHVERSQLLPCVDPSLPLEL----GVTCSDVVIDKLTNVWARLYVPM 83

Query: 68  SI---SSSKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLP 127
           +    S SKLP++ Y HGGGFC+GS +W     +  RL+A    LV+S +YRLAPEN LP
Sbjct: 84  TTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPLP 143

Query: 128 AAIDDGFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAAGLGLGSPE 187
           AA +DG  A+ WL  +A +D  + W A   DF R+F++GDSAGGNIA  +AA   L SPE
Sbjct: 144 AAYEDGVNAILWLN-KARND--NLW-AKQCDFGRIFLAGDSAGGNIAQQVAA--RLASPE 203

Query: 188 LAPVRVRGYVLLAPFFGGTVRTRSEAE--GSKEAFLNLELIDRFWRLSIPIGSSADHPLV 247
              +++ G +L+ PF+ G  RT SE      K A L L   D +WR+S+P G++ +HP  
Sbjct: 204 DLALKIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLPRGANREHPYC 263

Query: 248 DVFGPSSRNLKAVEMDPIMVVVGGADLLKDRAVEYAE 275
               P    +K+  +   +V V   DLL D  +E  +
Sbjct: 264 K---PVKMIIKSSTVTRTLVCVAEMDLLMDSNMEMCD 287

BLAST of Bhi10G000356 vs. ExPASy Swiss-Prot
Match: Q9LFR7 (Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.6e-44
Identity = 109/322 (33.85%), Postives = 172/322 (53.42%), Query Frame = 0

Query: 8   VDECRGVLRVYSDGSIVRSSNPSFNVPVFDDGSVLWKDLLFD--PIHDLHLRLYKP---A 67
           V+E  G+++V++DG + R        P     S   K   FD    +D   R+Y P   A
Sbjct: 29  VEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSS---KATAFDIKLSNDTWTRVYIPDAAA 88

Query: 68  SISSSKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLPAAI 127
           +  S  LP+L Y HGGGFC+GS  W    ++   LA +   +++S +YRLAPE+RLPAA 
Sbjct: 89  ASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPEHRLPAAY 148

Query: 128 DDGFAALRWL---QAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAAGLGLGSPE 187
           DDG   + WL   Q      +P  WL+   + S VF++GDSAG NIA+ +A  +      
Sbjct: 149 DDGVNVVSWLVKQQISTGGGYPS-WLSK-CNLSNVFLAGDSAGANIAYQVAVRIMASGKY 208

Query: 188 LAPVRVRGYVLLAPFFGGTVRTRSEAE--GSKEAFLNLELIDRFWRLSIPIGSSADHPLV 247
              + ++G +L+ PFFGG  RT SE +   +K + L L   D +WRL++P G+S DHP  
Sbjct: 209 ANTLHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRLALPRGASRDHPWC 268

Query: 248 DVFGPSSRNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQGKKIELVEFEGKQHGFFT 307
           +    S+      ++   MV +   D+LK+R +E  + ++  GK++E +   G  H F  
Sbjct: 269 NPLMSSA----GAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGGVGHAFHI 328

Query: 308 IDPNS---EASNQLMLLINHFI 317
           +D +S   +  + +M  +++FI
Sbjct: 329 LDNSSVSRDRIHDMMCRLHNFI 341

BLAST of Bhi10G000356 vs. ExPASy Swiss-Prot
Match: I4DST8 (Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana OX=13306 GN=TCEA1 PE=1 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 3.0e-43
Identity = 110/325 (33.85%), Postives = 164/325 (50.46%), Query Frame = 0

Query: 1   MATTPFEVD-----ECRGVLRVYSDGSIVRSSNPSFNVPVFDDGSVLWKDLLFDPIHDLH 60
           ++ TP  +D     + +  L +Y  G I R    +  +P   +  V  KD++ DP   + 
Sbjct: 72  LSPTPTALDDEIVLDLKPFLIIYKSGRIERFLGTTV-IPACPE--VATKDVVIDPATGVS 131

Query: 61  LRLYKP--ASISSSKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLA 120
           +RLY P    + S KLPVL Y HGGGF I +   PN  NY   LAA+   L++S +YRLA
Sbjct: 132 VRLYLPNVVDLPSKKLPVLVYFHGGGFVIENTGSPNYHNYLTLLAAKAGVLIVSINYRLA 191

Query: 121 PENRLPAAIDDGFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAAGL 180
           PE  LPA+ DD  A   W+ + +     +PWLA   DFS++ +SGDSAGGN+ H++A   
Sbjct: 192 PEYPLPASYDDCMAGFNWVVSHSAGPALEPWLAQHGDFSQILLSGDSAGGNVTHYVAMRA 251

Query: 181 GLGSPELAPVRVRGYVLLAPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSSAD 240
             G        + G  ++ P+F G+    +E         N+E  D+ WRL+ P     D
Sbjct: 252 DAGV-------IEGVAIVHPYFLGSEPVGNEINDP----ANIEFHDKLWRLAAPDTEGLD 311

Query: 241 HPLVDVFGPSSRNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQG--KKIELVEFEGK 300
            PL++   P + +L  ++    +V V G D L +R   Y E L K G   + ELV+ EG 
Sbjct: 312 DPLINPVAPGAPSLAGLKCKRAVVFVAGNDFLVERGRMYYEALVKSGWRGEAELVQHEGV 371

Query: 301 QHGFFTIDPNSEASNQLMLLINHFI 317
            H F   D + + S  +M  +  F+
Sbjct: 372 GHVFHLSDYSGDISVAMMTKLIAFL 382

BLAST of Bhi10G000356 vs. NCBI nr
Match: XP_038900705.1 (probable carboxylesterase 15 [Benincasa hispida])

HSP 1 Score: 659.8 bits (1701), Expect = 1.2e-185
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 0

Query: 1   MATTPFEVDECRGVLRVYSDGSIVRSSNPSFNVPVFDDGSVLWKDLLFDPIHDLHLRLYK 60
           MATTPFEVDECRGVLRVYSDGSIVRSSNPSFNVPVFDDGSVLWKDLLFDPIHDLHLRLYK
Sbjct: 1   MATTPFEVDECRGVLRVYSDGSIVRSSNPSFNVPVFDDGSVLWKDLLFDPIHDLHLRLYK 60

Query: 61  PASISSSKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLPA 120
           PASISSSKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLPA
Sbjct: 61  PASISSSKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLPA 120

Query: 121 AIDDGFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAAGLGLGSPEL 180
           AIDDGFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAAGLGLGSPEL
Sbjct: 121 AIDDGFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAAGLGLGSPEL 180

Query: 181 APVRVRGYVLLAPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSSADHPLVDVF 240
           APVRVRGYVLLAPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSSADHPLVDVF
Sbjct: 181 APVRVRGYVLLAPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSSADHPLVDVF 240

Query: 241 GPSSRNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQGKKIELVEFEGKQHGFFTIDP 300
           GPSSRNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQGKKIELVEFEGKQHGFFTIDP
Sbjct: 241 GPSSRNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQGKKIELVEFEGKQHGFFTIDP 300

Query: 301 NSEASNQLMLLINHFIAQHSL 322
           NSEASNQLMLLINHFIAQHSL
Sbjct: 301 NSEASNQLMLLINHFIAQHSL 321

BLAST of Bhi10G000356 vs. NCBI nr
Match: KAA0040076.1 (putative carboxylesterase 15 [Cucumis melo var. makuwa] >TYK21700.1 putative carboxylesterase 15 [Cucumis melo var. makuwa])

HSP 1 Score: 619.8 bits (1597), Expect = 1.3e-173
Identity = 298/317 (94.01%), Postives = 307/317 (96.85%), Query Frame = 0

Query: 5   PFEVDECRGVLRVYSDGSIVRSSNPSFNVPVFDDGSVLWKDLLFDPIHDLHLRLYKPASI 64
           P EVDECRGVLRVYSDGSIVRSSNPSFNVPV DDGSVLWKDLLFDPI+DLHLRLYKPA I
Sbjct: 4   PVEVDECRGVLRVYSDGSIVRSSNPSFNVPVNDDGSVLWKDLLFDPINDLHLRLYKPADI 63

Query: 65  SSSKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLPAAIDD 124
           SS KLPV FYIHGGGFCIGSRTWPNCQNYCFRLA++LPALVISPDYRLAPENRLPAAIDD
Sbjct: 64  SSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLPALVISPDYRLAPENRLPAAIDD 123

Query: 125 GFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAAGLGLGSPELAPVR 184
           GFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLA GLGLGSPELAPVR
Sbjct: 124 GFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAVGLGLGSPELAPVR 183

Query: 185 VRGYVLLAPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSSADHPLVDVFGPSS 244
           VRGYVLL PFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGS  DHPLV+VFGP+S
Sbjct: 184 VRGYVLLGPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSDTDHPLVNVFGPTS 243

Query: 245 RNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQGKKIELVEFEGKQHGFFTIDPNSEA 304
           RNL+AVEMDPIMVVV GADLLKDRAVEYAE+LKKQGKKI+LVEFEGKQHGFFTIDPNSEA
Sbjct: 244 RNLEAVEMDPIMVVVAGADLLKDRAVEYAEELKKQGKKIDLVEFEGKQHGFFTIDPNSEA 303

Query: 305 SNQLMLLINHFIAQHSL 322
           SNQLMLLINHF+AQHSL
Sbjct: 304 SNQLMLLINHFVAQHSL 320

BLAST of Bhi10G000356 vs. NCBI nr
Match: XP_008449839.1 (PREDICTED: probable carboxylesterase 15 [Cucumis melo])

HSP 1 Score: 616.7 bits (1589), Expect = 1.1e-172
Identity = 297/317 (93.69%), Postives = 306/317 (96.53%), Query Frame = 0

Query: 5   PFEVDECRGVLRVYSDGSIVRSSNPSFNVPVFDDGSVLWKDLLFDPIHDLHLRLYKPASI 64
           P EVDECRGVLRVYSDGSIVRSSNPSFNVPV DDGSVLWKDLLFDPI+DLHLRLYKPA I
Sbjct: 4   PVEVDECRGVLRVYSDGSIVRSSNPSFNVPVNDDGSVLWKDLLFDPINDLHLRLYKPADI 63

Query: 65  SSSKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLPAAIDD 124
           SS KL V FYIHGGGFCIGSRTWPNCQNYCFRLA++LPALVISPDYRLAPENRLPAAIDD
Sbjct: 64  SSPKLSVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLPALVISPDYRLAPENRLPAAIDD 123

Query: 125 GFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAAGLGLGSPELAPVR 184
           GFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLA GLGLGSPELAPVR
Sbjct: 124 GFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAVGLGLGSPELAPVR 183

Query: 185 VRGYVLLAPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSSADHPLVDVFGPSS 244
           VRGYVLL PFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGS  DHPLV+VFGP+S
Sbjct: 184 VRGYVLLGPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSDTDHPLVNVFGPTS 243

Query: 245 RNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQGKKIELVEFEGKQHGFFTIDPNSEA 304
           RNL+AVEMDPIMVVV GADLLKDRAVEYAE+LKKQGKKI+LVEFEGKQHGFFTIDPNSEA
Sbjct: 244 RNLEAVEMDPIMVVVAGADLLKDRAVEYAEELKKQGKKIDLVEFEGKQHGFFTIDPNSEA 303

Query: 305 SNQLMLLINHFIAQHSL 322
           SNQLMLLINHF+AQHSL
Sbjct: 304 SNQLMLLINHFVAQHSL 320

BLAST of Bhi10G000356 vs. NCBI nr
Match: XP_004142252.1 (probable carboxylesterase 15 [Cucumis sativus] >KGN54059.1 hypothetical protein Csa_018062 [Cucumis sativus])

HSP 1 Score: 602.1 bits (1551), Expect = 2.9e-168
Identity = 289/317 (91.17%), Postives = 302/317 (95.27%), Query Frame = 0

Query: 5   PFEVDECRGVLRVYSDGSIVRSSNPSFNVPVFDDGSVLWKDLLFDPIHDLHLRLYKPASI 64
           P EVDECRGVLRVYSDGSIVRSSNPSF+VPV DDGSVLWKDLLFDPIH+LHLRLYKPA I
Sbjct: 4   PIEVDECRGVLRVYSDGSIVRSSNPSFSVPVLDDGSVLWKDLLFDPIHNLHLRLYKPAHI 63

Query: 65  SSSKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLPAAIDD 124
           SS KLPV FYIHGGGFCIGSRTWPNCQNYCFRLA+EL ALVISPDYRLAPENRLPAAIDD
Sbjct: 64  SSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASELSALVISPDYRLAPENRLPAAIDD 123

Query: 125 GFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAAGLGLGSPELAPVR 184
           GFAALRWLQAQAESDHPDPWLA+VADFS VFISGDSAGGNIAHHLA GLG+GSPELAPV+
Sbjct: 124 GFAALRWLQAQAESDHPDPWLAEVADFSTVFISGDSAGGNIAHHLAVGLGVGSPELAPVQ 183

Query: 185 VRGYVLLAPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSSADHPLVDVFGPSS 244
           VRGYVLL PFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGS+ DHPLV+VFGP S
Sbjct: 184 VRGYVLLGPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVNVFGPRS 243

Query: 245 RNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQGKKIELVEFEGKQHGFFTIDPNSEA 304
            NL+AVEMDPI+VVV GADLLKDRAVEY E+LKKQGKKI+LVEFE KQHGFFTIDPNSEA
Sbjct: 244 LNLEAVEMDPIVVVVAGADLLKDRAVEYVEELKKQGKKIDLVEFEEKQHGFFTIDPNSEA 303

Query: 305 SNQLMLLINHFIAQHSL 322
           SNQLMLLINHF+AQHSL
Sbjct: 304 SNQLMLLINHFVAQHSL 320

BLAST of Bhi10G000356 vs. NCBI nr
Match: XP_023534075.1 (probable carboxylesterase 15 [Cucurbita pepo subsp. pepo] >KAG6605861.1 putative carboxylesterase 15, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035811.1 putative carboxylesterase 15, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 593.2 bits (1528), Expect = 1.3e-165
Identity = 289/321 (90.03%), Postives = 304/321 (94.70%), Query Frame = 0

Query: 1   MAT-TPFEVDECRGVLRVYSDGSIVRSSNPSFNVPVFDDGSVLWKDLLFDPIHDLHLRLY 60
           MAT +PFEVDECRGVLRVYSDGSIVRSSNPSFNVPV DDGSVLWKDLLFDPI+ LHLRLY
Sbjct: 1   MATSSPFEVDECRGVLRVYSDGSIVRSSNPSFNVPVLDDGSVLWKDLLFDPINHLHLRLY 60

Query: 61  KPASISSSKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLP 120
           KPA +SSS LPV FYIHGGGFCIGSRTWPNCQNYCFRLA ELPALVISPDYRLAPENRLP
Sbjct: 61  KPA-VSSSTLPVFFYIHGGGFCIGSRTWPNCQNYCFRLALELPALVISPDYRLAPENRLP 120

Query: 121 AAIDDGFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAAGLGLGSPE 180
           AAID GFAA+RWLQAQA+SDHPDPWLADVADFSRVFISGDSAGGNIAHHLA GLGLGSP 
Sbjct: 121 AAIDGGFAAVRWLQAQAQSDHPDPWLADVADFSRVFISGDSAGGNIAHHLAVGLGLGSPA 180

Query: 181 LAPVRVRGYVLLAPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSSADHPLVDV 240
           LAPVRVRGYVLLAPFFGGTVRTRSEAEG K+AFLNLELIDRFWRLSIPIGSS DHPLV+V
Sbjct: 181 LAPVRVRGYVLLAPFFGGTVRTRSEAEGPKDAFLNLELIDRFWRLSIPIGSSTDHPLVNV 240

Query: 241 FGPSSRNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQGKKIELVEFEGKQHGFFTID 300
            G SS++L+AVE+DPI+VVVGGADLLKDRAVEYAE LKKQGKKIELVEFEGKQHGFFTID
Sbjct: 241 LGQSSQDLEAVEVDPILVVVGGADLLKDRAVEYAETLKKQGKKIELVEFEGKQHGFFTID 300

Query: 301 PNSEASNQLMLLINHFIAQHS 321
           PNSEASNQLMLL+  F+A+HS
Sbjct: 301 PNSEASNQLMLLLKQFVARHS 320

BLAST of Bhi10G000356 vs. ExPASy TrEMBL
Match: A0A5A7TDB3 (Putative carboxylesterase 15 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00670 PE=4 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 6.5e-174
Identity = 298/317 (94.01%), Postives = 307/317 (96.85%), Query Frame = 0

Query: 5   PFEVDECRGVLRVYSDGSIVRSSNPSFNVPVFDDGSVLWKDLLFDPIHDLHLRLYKPASI 64
           P EVDECRGVLRVYSDGSIVRSSNPSFNVPV DDGSVLWKDLLFDPI+DLHLRLYKPA I
Sbjct: 4   PVEVDECRGVLRVYSDGSIVRSSNPSFNVPVNDDGSVLWKDLLFDPINDLHLRLYKPADI 63

Query: 65  SSSKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLPAAIDD 124
           SS KLPV FYIHGGGFCIGSRTWPNCQNYCFRLA++LPALVISPDYRLAPENRLPAAIDD
Sbjct: 64  SSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLPALVISPDYRLAPENRLPAAIDD 123

Query: 125 GFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAAGLGLGSPELAPVR 184
           GFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLA GLGLGSPELAPVR
Sbjct: 124 GFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAVGLGLGSPELAPVR 183

Query: 185 VRGYVLLAPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSSADHPLVDVFGPSS 244
           VRGYVLL PFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGS  DHPLV+VFGP+S
Sbjct: 184 VRGYVLLGPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSDTDHPLVNVFGPTS 243

Query: 245 RNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQGKKIELVEFEGKQHGFFTIDPNSEA 304
           RNL+AVEMDPIMVVV GADLLKDRAVEYAE+LKKQGKKI+LVEFEGKQHGFFTIDPNSEA
Sbjct: 244 RNLEAVEMDPIMVVVAGADLLKDRAVEYAEELKKQGKKIDLVEFEGKQHGFFTIDPNSEA 303

Query: 305 SNQLMLLINHFIAQHSL 322
           SNQLMLLINHF+AQHSL
Sbjct: 304 SNQLMLLINHFVAQHSL 320

BLAST of Bhi10G000356 vs. ExPASy TrEMBL
Match: A0A1S3BNL4 (probable carboxylesterase 15 OS=Cucumis melo OX=3656 GN=LOC103491598 PE=4 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 5.5e-173
Identity = 297/317 (93.69%), Postives = 306/317 (96.53%), Query Frame = 0

Query: 5   PFEVDECRGVLRVYSDGSIVRSSNPSFNVPVFDDGSVLWKDLLFDPIHDLHLRLYKPASI 64
           P EVDECRGVLRVYSDGSIVRSSNPSFNVPV DDGSVLWKDLLFDPI+DLHLRLYKPA I
Sbjct: 4   PVEVDECRGVLRVYSDGSIVRSSNPSFNVPVNDDGSVLWKDLLFDPINDLHLRLYKPADI 63

Query: 65  SSSKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLPAAIDD 124
           SS KL V FYIHGGGFCIGSRTWPNCQNYCFRLA++LPALVISPDYRLAPENRLPAAIDD
Sbjct: 64  SSPKLSVFFYIHGGGFCIGSRTWPNCQNYCFRLASQLPALVISPDYRLAPENRLPAAIDD 123

Query: 125 GFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAAGLGLGSPELAPVR 184
           GFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLA GLGLGSPELAPVR
Sbjct: 124 GFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAVGLGLGSPELAPVR 183

Query: 185 VRGYVLLAPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSSADHPLVDVFGPSS 244
           VRGYVLL PFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGS  DHPLV+VFGP+S
Sbjct: 184 VRGYVLLGPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSDTDHPLVNVFGPTS 243

Query: 245 RNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQGKKIELVEFEGKQHGFFTIDPNSEA 304
           RNL+AVEMDPIMVVV GADLLKDRAVEYAE+LKKQGKKI+LVEFEGKQHGFFTIDPNSEA
Sbjct: 244 RNLEAVEMDPIMVVVAGADLLKDRAVEYAEELKKQGKKIDLVEFEGKQHGFFTIDPNSEA 303

Query: 305 SNQLMLLINHFIAQHSL 322
           SNQLMLLINHF+AQHSL
Sbjct: 304 SNQLMLLINHFVAQHSL 320

BLAST of Bhi10G000356 vs. ExPASy TrEMBL
Match: A0A0A0KZ31 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G279870 PE=4 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 1.4e-168
Identity = 289/317 (91.17%), Postives = 302/317 (95.27%), Query Frame = 0

Query: 5   PFEVDECRGVLRVYSDGSIVRSSNPSFNVPVFDDGSVLWKDLLFDPIHDLHLRLYKPASI 64
           P EVDECRGVLRVYSDGSIVRSSNPSF+VPV DDGSVLWKDLLFDPIH+LHLRLYKPA I
Sbjct: 4   PIEVDECRGVLRVYSDGSIVRSSNPSFSVPVLDDGSVLWKDLLFDPIHNLHLRLYKPAHI 63

Query: 65  SSSKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLPAAIDD 124
           SS KLPV FYIHGGGFCIGSRTWPNCQNYCFRLA+EL ALVISPDYRLAPENRLPAAIDD
Sbjct: 64  SSPKLPVFFYIHGGGFCIGSRTWPNCQNYCFRLASELSALVISPDYRLAPENRLPAAIDD 123

Query: 125 GFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAAGLGLGSPELAPVR 184
           GFAALRWLQAQAESDHPDPWLA+VADFS VFISGDSAGGNIAHHLA GLG+GSPELAPV+
Sbjct: 124 GFAALRWLQAQAESDHPDPWLAEVADFSTVFISGDSAGGNIAHHLAVGLGVGSPELAPVQ 183

Query: 185 VRGYVLLAPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSSADHPLVDVFGPSS 244
           VRGYVLL PFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGS+ DHPLV+VFGP S
Sbjct: 184 VRGYVLLGPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSNTDHPLVNVFGPRS 243

Query: 245 RNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQGKKIELVEFEGKQHGFFTIDPNSEA 304
            NL+AVEMDPI+VVV GADLLKDRAVEY E+LKKQGKKI+LVEFE KQHGFFTIDPNSEA
Sbjct: 244 LNLEAVEMDPIVVVVAGADLLKDRAVEYVEELKKQGKKIDLVEFEEKQHGFFTIDPNSEA 303

Query: 305 SNQLMLLINHFIAQHSL 322
           SNQLMLLINHF+AQHSL
Sbjct: 304 SNQLMLLINHFVAQHSL 320

BLAST of Bhi10G000356 vs. ExPASy TrEMBL
Match: A0A6J1JYE3 (probable carboxylesterase 15 OS=Cucurbita maxima OX=3661 GN=LOC111490877 PE=4 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.5e-165
Identity = 288/321 (89.72%), Postives = 303/321 (94.39%), Query Frame = 0

Query: 1   MAT-TPFEVDECRGVLRVYSDGSIVRSSNPSFNVPVFDDGSVLWKDLLFDPIHDLHLRLY 60
           MAT +PFEVDECRGVLRVYSDGSIVRSSNPSFNVPV DDGSVLWKDLLFDPI+ LHLRLY
Sbjct: 1   MATSSPFEVDECRGVLRVYSDGSIVRSSNPSFNVPVLDDGSVLWKDLLFDPINHLHLRLY 60

Query: 61  KPASISSSKLPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLP 120
           KPA +SSS LPV FYIHGGGFCIGSRTWPNCQNYCFRLA ELPALVISPDYRLAPENRLP
Sbjct: 61  KPA-VSSSALPVFFYIHGGGFCIGSRTWPNCQNYCFRLALELPALVISPDYRLAPENRLP 120

Query: 121 AAIDDGFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAAGLGLGSPE 180
           AAIDDGF+A+RWLQAQA+SDHPDPWLADVADFSRVFISGDSAGGNIAHHLA GLGLGSP 
Sbjct: 121 AAIDDGFSAVRWLQAQAQSDHPDPWLADVADFSRVFISGDSAGGNIAHHLAVGLGLGSPA 180

Query: 181 LAPVRVRGYVLLAPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSSADHPLVDV 240
            APVRVRGYVLLAPFFGGTVRTRSEAEG K+AFLNLELIDRFWRLSIPIGSS DHPLV+V
Sbjct: 181 FAPVRVRGYVLLAPFFGGTVRTRSEAEGPKDAFLNLELIDRFWRLSIPIGSSTDHPLVNV 240

Query: 241 FGPSSRNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQGKKIELVEFEGKQHGFFTID 300
            G SS++L+AVE+DPI+VVVGGADLLKDRAVEYAE LKKQ KKIELVEFEGKQHGFFTID
Sbjct: 241 LGQSSQDLEAVEVDPILVVVGGADLLKDRAVEYAETLKKQVKKIELVEFEGKQHGFFTID 300

Query: 301 PNSEASNQLMLLINHFIAQHS 321
           PNSEASNQLMLL+  F+AQHS
Sbjct: 301 PNSEASNQLMLLLKQFVAQHS 320

BLAST of Bhi10G000356 vs. ExPASy TrEMBL
Match: A0A6J1DHZ6 (probable carboxylesterase 15 OS=Momordica charantia OX=3673 GN=LOC111021261 PE=4 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 1.0e-155
Identity = 269/321 (83.80%), Postives = 293/321 (91.28%), Query Frame = 0

Query: 2   ATTPFEVDECRGVLRVYSDGSIVRSSNPSFNVPVFDDGSVLWKDLLFDPIHDLHLRLYKP 61
           ++ PFEV+ECRGVLRVYSDGSIVRSSNPSF+VPV DDGSVLWKDLLFD I+ LH+RLYKP
Sbjct: 7   SSPPFEVEECRGVLRVYSDGSIVRSSNPSFDVPVLDDGSVLWKDLLFDLINRLHVRLYKP 66

Query: 62  ASISSSK--LPVLFYIHGGGFCIGSRTWPNCQNYCFRLAAELPALVISPDYRLAPENRLP 121
           A+ SSS   LP+ FYIHGGGFCIGSRTWPNCQNYCFRLA  L ALVISPDYRLAPENRLP
Sbjct: 67  ANCSSSTSLLPIFFYIHGGGFCIGSRTWPNCQNYCFRLALHLSALVISPDYRLAPENRLP 126

Query: 122 AAIDDGFAALRWLQAQAESDHPDPWLADVADFSRVFISGDSAGGNIAHHLAAGLGLGSPE 181
           AAI DGFAALRWLQAQA SDHPDPWL+ VADFSR+FISGDSAGGNIAHHLA GLG GSPE
Sbjct: 127 AAIHDGFAALRWLQAQALSDHPDPWLSHVADFSRIFISGDSAGGNIAHHLAVGLGAGSPE 186

Query: 182 LAPVRVRGYVLLAPFFGGTVRTRSEAEGSKEAFLNLELIDRFWRLSIPIGSSADHPLVDV 241
           LAP+RVRGYVLL PFFGGTVRTRSEAEG KEAFLNL+LIDRFWRLSIP GS+ DHPLV+V
Sbjct: 187 LAPLRVRGYVLLGPFFGGTVRTRSEAEGPKEAFLNLDLIDRFWRLSIPPGSTTDHPLVNV 246

Query: 242 FGPSSRNLKAVEMDPIMVVVGGADLLKDRAVEYAEKLKKQGKKIELVEFEGKQHGFFTID 301
            GP SR+L+AVE+DP++VVVGG DLLKDRAVEYAEKLK+QGK IELVEFEGKQHGFFTI 
Sbjct: 247 LGPWSRDLEAVELDPMLVVVGGGDLLKDRAVEYAEKLKEQGKMIELVEFEGKQHGFFTIH 306

Query: 302 PNSEASNQLMLLINHFIAQHS 321
           PNSEA+NQLML++N FI Q+S
Sbjct: 307 PNSEAANQLMLIVNRFITQNS 327

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G06570.11.6e-9251.10alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.21.6e-9251.10alpha/beta-Hydrolases superfamily protein [more]
AT1G68620.13.0e-4639.35alpha/beta-Hydrolases superfamily protein [more]
AT5G16080.12.5e-4533.85carboxyesterase 17 [more]
AT5G23530.15.3e-4339.19carboxyesterase 18 [more]
Match NameE-valueIdentityDescription
Q9FG132.3e-9151.10Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1[more]
Q0ZPV71.3e-4635.20Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1[more]
Q9SX254.2e-4539.35Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1[more]
Q9LFR73.6e-4433.85Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1[more]
I4DST83.0e-4333.85Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana OX=13306 GN... [more]
Match NameE-valueIdentityDescription
XP_038900705.11.2e-185100.00probable carboxylesterase 15 [Benincasa hispida][more]
KAA0040076.11.3e-17394.01putative carboxylesterase 15 [Cucumis melo var. makuwa] >TYK21700.1 putative car... [more]
XP_008449839.11.1e-17293.69PREDICTED: probable carboxylesterase 15 [Cucumis melo][more]
XP_004142252.12.9e-16891.17probable carboxylesterase 15 [Cucumis sativus] >KGN54059.1 hypothetical protein ... [more]
XP_023534075.11.3e-16590.03probable carboxylesterase 15 [Cucurbita pepo subsp. pepo] >KAG6605861.1 putative... [more]
Match NameE-valueIdentityDescription
A0A5A7TDB36.5e-17494.01Putative carboxylesterase 15 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3BNL45.5e-17393.69probable carboxylesterase 15 OS=Cucumis melo OX=3656 GN=LOC103491598 PE=4 SV=1[more]
A0A0A0KZ311.4e-16891.17Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G2798... [more]
A0A6J1JYE32.5e-16589.72probable carboxylesterase 15 OS=Cucurbita maxima OX=3661 GN=LOC111490877 PE=4 SV... [more]
A0A6J1DHZ61.0e-15583.80probable carboxylesterase 15 OS=Momordica charantia OX=3673 GN=LOC111021261 PE=4... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 15..320
e-value: 4.2E-83
score: 281.5
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 5..319
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 72..296
e-value: 1.5E-58
score: 198.2
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 8..316
NoneNo IPR availablePANTHERPTHR23024:SF416CARBOXYLESTERASE 15-RELATEDcoord: 8..316
IPR033140Lipase, GDXG, putative serine active sitePROSITEPS01174LIPASE_GDXG_SERcoord: 154..166

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi10M000356Bhi10M000356mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity