Bhi10G000292 (gene) Wax gourd (B227) v1

Overview
NameBhi10G000292
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionLipoxygenase
Locationchr10: 8074500 .. 8075003 (-)
RNA-Seq ExpressionBhi10G000292
SyntenyBhi10G000292
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGAAATCTCAAGGCCTTGGGTCTTCAACAAGAGTGGCTCCGGCAACTCTCTGCCATATGCGGAGGTCGTTGGGATGTTGCAGCATCGACGGCGGTGCTTTGGTTCCTTACCAGAATAATAATAATCTCCAAAAGCAGCTGCGGGGGATTAAGATCAAGAATAATAATGGAGGTTTGAGACTAACGTCTAGGGGTGGAATAGCCAAAGCAGCCTTAGCCACGGCCACGGAACCCACCACTAGTGTCACTACAAAAGTTACTGTGAAGAAGATCACAGGAGAAATCACTTCATCCAAAATTTTAAAAGAAAACCAAGCCCCTCAAAAGTTGCTCCAGCTTGGGTTTGCCAGCATCCTCCTCGACCCTAGTAAGTTTCTCTTCCTTACACATTTAATTAATTATCTAAACAAAAACTATACTCTTTTATTTTATAATAATGTGAAACCTTTTTTAACCTTCATACGTACAAACACATCACTTGTTTTGTTTCCAAACCAGTAG

mRNA sequence

ATGTTGAAATCTCAAGGCCTTGGGTCTTCAACAAGAGTGGCTCCGGCAACTCTCTGCCATATGCGGAGGTCGTTGGGATGTTGCAGCATCGACGGCGGTGCTTTGGTTCCTTACCAGAATAATAATAATCTCCAAAAGCAGCTGCGGGGGATTAAGATCAAGAATAATAATGGAGGTTTGAGACTAACGTCTAGGGGTGGAATAGCCAAAGCAGCCTTAGCCACGGCCACGGAACCCACCACTAGTGTCACTACAAAAGTTACTGTGAAGAAGATCACAGGAGAAATCACTTCATCCAAAATTTTAAAAGAAAACCAAGCCCCTCAAAAGTTGCTCCAGCTTGGGTTTGCCAGCATCCTCCTCGACCCTAGTAAGTTTCTCTTCCTTACACATTTAATTAATTATCTAAACAAAAACTATACTCTTTTATTTTATAATAATGTGAAACCTTTTTTAACCTTCATACGTACAAACACATCACTTGTTTTGTTTCCAAACCAGTAG

Coding sequence (CDS)

ATGTTGAAATCTCAAGGCCTTGGGTCTTCAACAAGAGTGGCTCCGGCAACTCTCTGCCATATGCGGAGGTCGTTGGGATGTTGCAGCATCGACGGCGGTGCTTTGGTTCCTTACCAGAATAATAATAATCTCCAAAAGCAGCTGCGGGGGATTAAGATCAAGAATAATAATGGAGGTTTGAGACTAACGTCTAGGGGTGGAATAGCCAAAGCAGCCTTAGCCACGGCCACGGAACCCACCACTAGTGTCACTACAAAAGTTACTGTGAAGAAGATCACAGGAGAAATCACTTCATCCAAAATTTTAAAAGAAAACCAAGCCCCTCAAAAGTTGCTCCAGCTTGGGTTTGCCAGCATCCTCCTCGACCCTAGTAAGTTTCTCTTCCTTACACATTTAATTAATTATCTAAACAAAAACTATACTCTTTTATTTTATAATAATGTGAAACCTTTTTTAACCTTCATACGTACAAACACATCACTTGTTTTGTTTCCAAACCAGTAG

Protein sequence

MLKSQGLGSSTRVAPATLCHMRRSLGCCSIDGGALVPYQNNNNLQKQLRGIKIKNNNGGLRLTSRGGIAKAALATATEPTTSVTTKVTVKKITGEITSSKILKENQAPQKLLQLGFASILLDPSKFLFLTHLINYLNKNYTLLFYNNVKPFLTFIRTNTSLVLFPNQ
Homology
BLAST of Bhi10G000292 vs. NCBI nr
Match: XP_038901038.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 184.5 bits (467), Expect = 7.5e-43
Identity = 104/123 (84.55%), Postives = 111/123 (90.24%), Query Frame = 0

Query: 1   MLKSQGLGSSTRVAPATLCHMRRSLGCCSIDGGALVPYQNNNNLQKQLRGIKIKNNNGGL 60
           MLKSQG GSSTRVAPATLCH++R LG  SI+GGALV Y+NNNNL+KQL+GIKIK  NGGL
Sbjct: 1   MLKSQGFGSSTRVAPATLCHLQRPLG-HSINGGALVAYRNNNNLEKQLKGIKIK-KNGGL 60

Query: 61  RLTSRGGIAKAALATATEPTTSVTTKVTVKKITGEITSSKILKENQAPQKLLQLGFASIL 120
           RL S GGI KAALATATEPTT+V TKV VKKITGEITSSKILKENQAPQKLLQLGFASIL
Sbjct: 61  RLVSGGGIIKAALATATEPTTTVITKVIVKKITGEITSSKILKENQAPQKLLQLGFASIL 120

Query: 121 LDP 124
           LDP
Sbjct: 121 LDP 121

BLAST of Bhi10G000292 vs. NCBI nr
Match: XP_004142137.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic [Cucumis sativus] >KGN54488.2 hypothetical protein Csa_018102 [Cucumis sativus])

HSP 1 Score: 160.6 bits (405), Expect = 1.2e-35
Identity = 97/128 (75.78%), Postives = 101/128 (78.91%), Query Frame = 0

Query: 1   MLKSQGLGSSTRVAPATLCHMRRSLGCCSIDGGALVPYQNNNNLQKQLRGIKIKNN--NG 60
           MLKSQGLGSS  VAP +L H R SL    IDG ALVPYQNNN+LQK+LRGIKIKNN  NG
Sbjct: 1   MLKSQGLGSSIGVAPVSLHHTRTSLARRGIDGSALVPYQNNNDLQKKLRGIKIKNNNDNG 60

Query: 61  GLRLTSRGGIAKAALATATEPTTSVTTKVTVKKITGEITSSKILKENQ--APQKLLQLGF 120
           GLR  S  GI KAALATATE TTSV T VTVKKITGEITSS I+KENQ  AP K LQLGF
Sbjct: 61  GLRSLSGSGIIKAALATATETTTSVITTVTVKKITGEITSSTIVKENQLAAPPKFLQLGF 120

Query: 121 ASILLDPS 125
           ASIL DPS
Sbjct: 121 ASILTDPS 128

BLAST of Bhi10G000292 vs. NCBI nr
Match: KAA0041376.1 (linoleate 13S-lipoxygenase 2-1 [Cucumis melo var. makuwa])

HSP 1 Score: 154.5 bits (389), Expect = 8.3e-34
Identity = 98/129 (75.97%), Postives = 104/129 (80.62%), Query Frame = 0

Query: 1   MLKSQGLGSSTRVAPATLCHMRRSLG-CCSIDGGALVPYQNNNNLQKQLRGIKIKN--NN 60
           MLKSQGLGSS  VAP TL HM+ SL    +IDG ALVPYQNNN+LQK+LRGIKIKN  NN
Sbjct: 1   MLKSQGLGSSIGVAPVTLRHMQTSLARRRNIDGSALVPYQNNNDLQKKLRGIKIKNNDNN 60

Query: 61  GGLRLTS-RGGIAKAALATATEPTTSVTTKVTVKKITGEITSSKILKENQ-APQKLLQLG 120
           GGLRL S   G  KAALATATE TTSV TKV VKKITGEITSS I+KENQ A QK+LQLG
Sbjct: 61  GGLRLMSGPSGTIKAALATATELTTSVITKVVVKKITGEITSSTIIKENQLATQKVLQLG 120

Query: 121 FASILLDPS 125
           FASIL DPS
Sbjct: 121 FASILTDPS 129

BLAST of Bhi10G000292 vs. NCBI nr
Match: XP_008449749.1 (PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis melo] >TYK21640.1 linoleate 13S-lipoxygenase 2-1 [Cucumis melo var. makuwa])

HSP 1 Score: 154.5 bits (389), Expect = 8.3e-34
Identity = 98/129 (75.97%), Postives = 104/129 (80.62%), Query Frame = 0

Query: 1   MLKSQGLGSSTRVAPATLCHMRRSLG-CCSIDGGALVPYQNNNNLQKQLRGIKIKN--NN 60
           MLKSQGLGSS  VAP TL HM+ SL    +IDG ALVPYQNNN+LQK+LRGIKIKN  NN
Sbjct: 1   MLKSQGLGSSIGVAPVTLRHMQTSLARRRNIDGSALVPYQNNNDLQKKLRGIKIKNNDNN 60

Query: 61  GGLRLTS-RGGIAKAALATATEPTTSVTTKVTVKKITGEITSSKILKENQ-APQKLLQLG 120
           GGLRL S   G  KAALATATE TTSV TKV VKKITGEITSS I+KENQ A QK+LQLG
Sbjct: 61  GGLRLMSGPSGTIKAALATATELTTSVITKVVVKKITGEITSSTIIKENQLATQKVLQLG 120

Query: 121 FASILLDPS 125
           FASIL DPS
Sbjct: 121 FASILTDPS 129

BLAST of Bhi10G000292 vs. NCBI nr
Match: NP_001315401.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis melo] >AME15769.1 lipoxygenase 13 [Cucumis melo var. makuwa] >KAA0041375.1 linoleate 13S-lipoxygenase 2-1 [Cucumis melo var. makuwa])

HSP 1 Score: 136.7 bits (343), Expect = 1.8e-28
Identity = 82/123 (66.67%), Postives = 92/123 (74.80%), Query Frame = 0

Query: 1   MLKSQGLGSSTRVAPATLCHMRRSLGCCSIDGGALVPYQNNNNLQKQLRGIKIKNNNGGL 60
           MLKS   GSST+VA  TLCH+RRS    +  G    PY N+ NL+KQLRG K K NNG L
Sbjct: 1   MLKSPACGSSTQVALVTLCHLRRS---SNRSGALFQPYPNDINLKKQLRGKKSK-NNGVL 60

Query: 61  RLTSRGGIAKAALATATEPTTSVTTKVTVKKITGEITSSKILKENQAPQKLLQLGFASIL 120
           RL S GGI KAALATA EPTT+V TKV VKKITGE TSS +++ +Q PQKLLQLGFASIL
Sbjct: 61  RLVSGGGIIKAALATAAEPTTTVITKVIVKKITGETTSSNLMEASQPPQKLLQLGFASIL 119

Query: 121 LDP 124
           LDP
Sbjct: 121 LDP 119

BLAST of Bhi10G000292 vs. ExPASy TrEMBL
Match: A0A0A0L0L3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G288080 PE=3 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 5.6e-36
Identity = 97/128 (75.78%), Postives = 101/128 (78.91%), Query Frame = 0

Query: 1   MLKSQGLGSSTRVAPATLCHMRRSLGCCSIDGGALVPYQNNNNLQKQLRGIKIKNN--NG 60
           MLKSQGLGSS  VAP +L H R SL    IDG ALVPYQNNN+LQK+LRGIKIKNN  NG
Sbjct: 1   MLKSQGLGSSIGVAPVSLHHTRTSLARRGIDGSALVPYQNNNDLQKKLRGIKIKNNNDNG 60

Query: 61  GLRLTSRGGIAKAALATATEPTTSVTTKVTVKKITGEITSSKILKENQ--APQKLLQLGF 120
           GLR  S  GI KAALATATE TTSV T VTVKKITGEITSS I+KENQ  AP K LQLGF
Sbjct: 61  GLRSLSGSGIIKAALATATETTTSVITTVTVKKITGEITSSTIVKENQLAAPPKFLQLGF 120

Query: 121 ASILLDPS 125
           ASIL DPS
Sbjct: 121 ASILTDPS 128

BLAST of Bhi10G000292 vs. ExPASy TrEMBL
Match: A0A5D3DDW6 (Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00020 PE=3 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 4.0e-34
Identity = 98/129 (75.97%), Postives = 104/129 (80.62%), Query Frame = 0

Query: 1   MLKSQGLGSSTRVAPATLCHMRRSLG-CCSIDGGALVPYQNNNNLQKQLRGIKIKN--NN 60
           MLKSQGLGSS  VAP TL HM+ SL    +IDG ALVPYQNNN+LQK+LRGIKIKN  NN
Sbjct: 1   MLKSQGLGSSIGVAPVTLRHMQTSLARRRNIDGSALVPYQNNNDLQKKLRGIKIKNNDNN 60

Query: 61  GGLRLTS-RGGIAKAALATATEPTTSVTTKVTVKKITGEITSSKILKENQ-APQKLLQLG 120
           GGLRL S   G  KAALATATE TTSV TKV VKKITGEITSS I+KENQ A QK+LQLG
Sbjct: 61  GGLRLMSGPSGTIKAALATATELTTSVITKVVVKKITGEITSSTIIKENQLATQKVLQLG 120

Query: 121 FASILLDPS 125
           FASIL DPS
Sbjct: 121 FASILTDPS 129

BLAST of Bhi10G000292 vs. ExPASy TrEMBL
Match: A0A5A7TH45 (Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00210 PE=3 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 4.0e-34
Identity = 98/129 (75.97%), Postives = 104/129 (80.62%), Query Frame = 0

Query: 1   MLKSQGLGSSTRVAPATLCHMRRSLG-CCSIDGGALVPYQNNNNLQKQLRGIKIKN--NN 60
           MLKSQGLGSS  VAP TL HM+ SL    +IDG ALVPYQNNN+LQK+LRGIKIKN  NN
Sbjct: 1   MLKSQGLGSSIGVAPVTLRHMQTSLARRRNIDGSALVPYQNNNDLQKKLRGIKIKNNDNN 60

Query: 61  GGLRLTS-RGGIAKAALATATEPTTSVTTKVTVKKITGEITSSKILKENQ-APQKLLQLG 120
           GGLRL S   G  KAALATATE TTSV TKV VKKITGEITSS I+KENQ A QK+LQLG
Sbjct: 61  GGLRLMSGPSGTIKAALATATELTTSVITKVVVKKITGEITSSTIIKENQLATQKVLQLG 120

Query: 121 FASILLDPS 125
           FASIL DPS
Sbjct: 121 FASILTDPS 129

BLAST of Bhi10G000292 vs. ExPASy TrEMBL
Match: A0A1S3BMR0 (Lipoxygenase OS=Cucumis melo OX=3656 GN=LOC103491540 PE=3 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 4.0e-34
Identity = 98/129 (75.97%), Postives = 104/129 (80.62%), Query Frame = 0

Query: 1   MLKSQGLGSSTRVAPATLCHMRRSLG-CCSIDGGALVPYQNNNNLQKQLRGIKIKN--NN 60
           MLKSQGLGSS  VAP TL HM+ SL    +IDG ALVPYQNNN+LQK+LRGIKIKN  NN
Sbjct: 1   MLKSQGLGSSIGVAPVTLRHMQTSLARRRNIDGSALVPYQNNNDLQKKLRGIKIKNNDNN 60

Query: 61  GGLRLTS-RGGIAKAALATATEPTTSVTTKVTVKKITGEITSSKILKENQ-APQKLLQLG 120
           GGLRL S   G  KAALATATE TTSV TKV VKKITGEITSS I+KENQ A QK+LQLG
Sbjct: 61  GGLRLMSGPSGTIKAALATATELTTSVITKVVVKKITGEITSSTIIKENQLATQKVLQLG 120

Query: 121 FASILLDPS 125
           FASIL DPS
Sbjct: 121 FASILTDPS 129

BLAST of Bhi10G000292 vs. ExPASy TrEMBL
Match: A0A125S6K8 (Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=LOX13 PE=2 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 8.7e-29
Identity = 82/123 (66.67%), Postives = 92/123 (74.80%), Query Frame = 0

Query: 1   MLKSQGLGSSTRVAPATLCHMRRSLGCCSIDGGALVPYQNNNNLQKQLRGIKIKNNNGGL 60
           MLKS   GSST+VA  TLCH+RRS    +  G    PY N+ NL+KQLRG K K NNG L
Sbjct: 1   MLKSPACGSSTQVALVTLCHLRRS---SNRSGALFQPYPNDINLKKQLRGKKSK-NNGVL 60

Query: 61  RLTSRGGIAKAALATATEPTTSVTTKVTVKKITGEITSSKILKENQAPQKLLQLGFASIL 120
           RL S GGI KAALATA EPTT+V TKV VKKITGE TSS +++ +Q PQKLLQLGFASIL
Sbjct: 61  RLVSGGGIIKAALATAAEPTTTVITKVIVKKITGETTSSNLMEASQPPQKLLQLGFASIL 119

Query: 121 LDP 124
           LDP
Sbjct: 121 LDP 119

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_038901038.17.5e-4384.55linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Benincasa hispida][more]
XP_004142137.11.2e-3575.78linoleate 13S-lipoxygenase 2-1, chloroplastic [Cucumis sativus] >KGN54488.2 hypo... [more]
KAA0041376.18.3e-3475.97linoleate 13S-lipoxygenase 2-1 [Cucumis melo var. makuwa][more]
XP_008449749.18.3e-3475.97PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis melo] >TY... [more]
NP_001315401.11.8e-2866.67linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis melo] >AME15769.1 li... [more]
Match NameE-valueIdentityDescription
A0A0A0L0L35.6e-3675.78Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G288080 PE=3 SV=1[more]
A0A5D3DDW64.0e-3475.97Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00020 P... [more]
A0A5A7TH454.0e-3475.97Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00210 P... [more]
A0A1S3BMR04.0e-3475.97Lipoxygenase OS=Cucumis melo OX=3656 GN=LOC103491540 PE=3 SV=1[more]
A0A125S6K88.7e-2966.67Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=LOX13 PE=2 SV=1[more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi10M000292Bhi10M000292mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0031408 oxylipin biosynthetic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen