Bhi09G002411 (gene) Wax gourd (B227) v1

BLAST of Bhi09G002411 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 756.9 bits (1953), Expect = 1.1e-218
Identity = 355/552 (64.31%), Postives = 448/552 (81.16%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           MEGT +  AN VPLTPI+FL+R+A V+ DR+S+VYG+V++TWR+T +RC ++ASAL QLG
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           IS  DVV+VLAPNVPA  ELHF VPMA A+LCTLN+RHD+++V+ LL HS  K+I  D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 FLSIVKAAIKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDFK 180
           FL I + A +I+S    K +P +V++ E    + +++  + +   +EYE ++  GK DF+
Sbjct: 123 FLQIAEGACEILSNKGDK-VPILVLIPE--PLTQSVSRKKRSEEMMEYEDVVAMGKSDFE 182

Query: 181 IKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMFH 240
           +  P DE D IS+NYTSGTTS PKGV+YSHRG YLN+L+ +LLN+M S P YLWT PMFH
Sbjct: 183 VIRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFH 242

Query: 241 CNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQKS 300
           CNGW L WGV A GGTNIC RNVTAK IF NIS HKVTHMGGAPT+LNMIINAP +EQK 
Sbjct: 243 CNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKP 302

Query: 301 LPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQA 360
           LP KV+ +TG APPP+HV++KM E GF + H YGLTET G  T+C WKPEWD+LP ++QA
Sbjct: 303 LPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQA 362

Query: 361 KLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAFN 420
           K+K+RQG+ H+GLE +++KDP+TM ++PADG TMGEV+FRGNTVM+GY K+ +AT+EAF 
Sbjct: 363 KMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFK 422

Query: 421 GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRPD 480
           GGWF SGDLGV+HPDGYIELKDRSKDIIISGGENISSIEVES LF HP VLEAA+V RPD
Sbjct: 423 GGWFWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPD 482

Query: 481 DHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGKIQKLILK 540
           ++WGET CAFV LKDG  A+ EE+I +CRD LPHYMAP+++VF+DLPK+STGK+QK +L+
Sbjct: 483 EYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLR 542

Query: 541 EEAKAMDSLTKR 553
            +AKA+ SL+K+
Sbjct: 543 TKAKALVSLSKK 551

BLAST of Bhi09G002411 vs. TAIR 10
Match: AT2G17650.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 723.4 bits (1866), Expect = 1.4e-208
Identity = 348/556 (62.59%), Postives = 435/556 (78.24%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           +EG  R  AN  PL+PITFLER+A V+ DR SLV+G+V+ TW +T +RC +LASAL  LG
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLG 107

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           ISR DVVA LAPNVPA +ELHFAVPMA  +LC LN R D + +S LL+HSEAKI+ VD+Q
Sbjct: 108 ISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQ 167

Query: 121 FLSIVKAAIKIMSKT--TTKELPRIVIVKEFDHPSSNINGFESASN----DLEYESLLDT 180
            L I   A+ +++K+  T K L  ++I +  D   S+ +   + ++    D EYE+LL +
Sbjct: 168 LLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKS 227

Query: 181 GKLDFKIKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLW 240
           G  +F+I  P+ EWDPIS+NYTSGTTSRPKGV+YSHRG YLN+L+T+ L+ MS  PVYLW
Sbjct: 228 GDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVYLW 287

Query: 241 TVPMFHCNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAP 300
           TVPMFHCNGW L WGVAAQGGTNIC R V+ K IF NI++HKVTHMGGAPTVLNMI+N  
Sbjct: 288 TVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYT 347

Query: 301 INEQKSLPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDAL 360
           + E K LP +V +MTGG+PP   +L KM E GF + H YGLTET G  T C WKPEWD+L
Sbjct: 348 VTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSL 407

Query: 361 PDDKQAKLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKA 420
             +++ KLK+RQG+QH+GLEG+++KDP+TME+VP DG TMGEVMFRGNTVMSGYFKD +A
Sbjct: 408 SLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEA 467

Query: 421 TQEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAA 480
           T++AF G WF SGDL V++PDGYIE+KDR KD+IISGGENISS+EVE VL +H +VLEAA
Sbjct: 468 TRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLEAA 527

Query: 481 IVGRPDDHWGETPCAFVNLKDGFNATE-EEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGK 540
           +V RPD HWG+TPC FV LK+GF+  + EEII FCRD+LPHYMAPKT+VF D+PK+STGK
Sbjct: 528 VVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTSTGK 587

Query: 541 IQKLILKEEAKAMDSL 550
           +QK +L+++A  M SL
Sbjct: 588 VQKYLLRKKADEMGSL 603

BLAST of Bhi09G002411 vs. TAIR 10
Match: AT1G20560.2 (acyl activating enzyme 1 )

HSP 1 Score: 652.5 bits (1682), Expect = 3.0e-187
Identity = 305/474 (64.35%), Postives = 383/474 (80.80%), Query Frame = 0

Query: 79  ELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQFLSIVKAAIKIMSKTTTK 138
           ELHF VPMA A+LCTLN+RHD+++V+ LL HS  K+I  D+QFL I + A +I+S    K
Sbjct: 3   ELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIAEGACEILSNKGDK 62

Query: 139 ELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDFKIKHPKDEWDPISLNYTSG 198
            +P +V++ E    + +++  + +   +EYE ++  GK DF++  P DE D IS+NYTSG
Sbjct: 63  -VPILVLIPE--PLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPTDECDAISVNYTSG 122

Query: 199 TTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMFHCNGWSLTWGVAAQGGTNI 258
           TTS PKGV+YSHRG YLN+L+ +LLN+M S P YLWT PMFHCNGW L WGV A GGTNI
Sbjct: 123 TTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNGWCLLWGVTAIGGTNI 182

Query: 259 CQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQKSLPCKVTVMTGGAPPPSHV 318
           C RNVTAK IF NIS HKVTHMGGAPT+LNMIINAP +EQK LP KV+ +TG APPP+HV
Sbjct: 183 CLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPGKVSFITGAAPPPAHV 242

Query: 319 LYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQAKLKSRQGMQHIGLEGVEI 378
           ++KM E GF + H YGLTET G  T+C WKPEWD+LP ++QAK+K+RQG+ H+GLE +++
Sbjct: 243 IFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMKARQGVNHLGLEEIQV 302

Query: 379 KDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAFNGGWFRSGDLGVRHPDGYI 438
           KDP+TM ++PADG TMGEV+FRGNTVM+GY K+ +AT+EAF GGWF SGDLGV+HPDGYI
Sbjct: 303 KDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGWFWSGDLGVKHPDGYI 362

Query: 439 ELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRPDDHWGETPCAFVNLKDGFN 498
           ELKDRSKDIIISGGENISSIEVES LF HP VLEAA+V RPD++WGET CAFV LKDG  
Sbjct: 363 ELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAFVKLKDGSK 422

Query: 499 ATEEEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGKIQKLILKEEAKAMDSLTKR 553
           A+ EE+I +CRD LPHYMAP+++VF+DLPK+STGK+QK +L+ +AKA+ SL+K+
Sbjct: 423 ASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKALVSLSKK 473

BLAST of Bhi09G002411 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 578.6 bits (1490), Expect = 5.5e-165
Identity = 278/554 (50.18%), Postives = 390/554 (70.40%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           M+    C AN VPLTPITFL+RA+  + +R S++YG  RFTW +T +RC +LA++L+ L 
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           I+R DVV++LAPNVPA YE+HF+VPM  AVL  +N R DA  ++ +L H+E KI+ VDY+
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 121 FLSIVKAAIKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGK---- 180
           F  +++  ++++    ++  PRI+++ E D  +      +  S +L+YE L+  G+    
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTT------KPFSKELDYEGLIRKGEPTPS 180

Query: 181 LDFKIKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTV 240
               +    +E DPISLNYTSGTT+ PKGV+ SH+G YL+ LS+I+  +M   PVYLWT+
Sbjct: 181 SSASMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTL 240

Query: 241 PMFHCNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPIN 300
           PMFHCNGW+ TW VAA+GGTN+C R+VTA EI+ NI LH VTHM   PTV   ++     
Sbjct: 241 PMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRT 300

Query: 301 EQKSLPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPD 360
           +Q      V V+TGG+ PP+ ++ K+ + GF ++H YGLTE  G    C W+ EW+ LP+
Sbjct: 301 DQSPKSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPE 360

Query: 361 DKQAKLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQ 420
            +Q +L+ RQG++++ L  V++K+  T+ESVP DGKTMGE++ +G+++M GY K+ KAT 
Sbjct: 361 HQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATS 420

Query: 421 EAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIV 480
           EAF  GW  +GD+GV HPDGY+E+KDRSKDIIISGGENISSIEVE VL+ +  VLEAA+V
Sbjct: 421 EAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVV 480

Query: 481 GRPDDHWGETPCAFVNLK---DGFNATEEEIIKFCRDNLPHYMAPKTVV-FKDLPKSSTG 540
             P   WGETPCAFV LK   +G   +E ++IK+CR+N+PH+M PK VV F++LPK+S G
Sbjct: 481 AMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNG 540

Query: 541 KIQKLILKEEAKAM 547
           KI K  L++ AKA+
Sbjct: 541 KILKSKLRDIAKAL 548

BLAST of Bhi09G002411 vs. TAIR 10
Match: AT1G76290.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 572.0 bits (1473), Expect = 5.2e-163
Identity = 285/558 (51.08%), Postives = 383/558 (68.64%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           MEGT RCSAN VPL+PI+FLERAA VFG R S+VYG++++TW +T +RC +LASAL  LG
Sbjct: 1   MEGTMRCSANYVPLSPISFLERAAVVFGSRTSVVYGDIQYTWHQTRDRCVRLASALSDLG 60

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           +SR DVVA LAPNVPA  EL+F  PMA AVLC LN   D+ M++  L  ++ K+  VD +
Sbjct: 61  LSRHDVVAALAPNVPALCELYFGAPMAGAVLCVLNTTFDSQMLAMALEKTKPKVFFVDSE 120

Query: 121 FLSIVKAAIKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDFK 180
           FLS+ + ++ ++S    +E P I+ + E        N  E +  + +YE  L TG  +FK
Sbjct: 121 FLSVAEESLSLLS--NIEEKPLIITITE--------NPTEQSKYE-QYEDFLSTGNPNFK 180

Query: 181 IKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMFH 240
              P DE DPI+LN+TSGTTS PK V+YSHRG YLN  +  ++N+M  MPVYL TVPM+H
Sbjct: 181 PIRPVDECDPIALNFTSGTTSTPKCVVYSHRGAYLNATAVGVMNEMKPMPVYLCTVPMYH 240

Query: 241 CNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQKS 300
           C+GW   W V A GG  +C R V  + IF +I  HKVT+ GG+P VLNMI NA  + +KS
Sbjct: 241 CSGWCYIWTVTAFGGVIVCLREVNDEVIFDSIVKHKVTNFGGSPPVLNMIANARDSVKKS 300

Query: 301 LPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQA 360
            P  V VM+GG+ PP  V+ K+++ GF ++  YG +E  G  T C W PEW+ LP+++  
Sbjct: 301 FPWTVQVMSGGSSPP-EVMLKLKKLGFKVMMAYGCSEVYGLGTACLWMPEWETLPEEESL 360

Query: 361 KLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAFN 420
           +LK+R G+ H   E V++ DP TM+SVP DGKT+  +  RGNTVMSGYFKD +AT+ AF 
Sbjct: 361 RLKARDGLNHFAKEAVDVLDPTTMKSVPHDGKTIRVIALRGNTVMSGYFKDKEATEAAFR 420

Query: 421 GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRPD 480
           GGW+ S D+GV  PDGYI+ KDRS+D+I  GGE + S E+E +L++HP+V +A +VGRPD
Sbjct: 421 GGWYWSRDMGVIDPDGYIQFKDRSQDVITCGGEIVGSKEIEGILYSHPAVYDAGVVGRPD 480

Query: 481 DHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPH---YMAPKTVVFKDLPKSSTGKIQKL 540
           +  GE+ CAFV LK+G  A EEEII+FC+  L +    M PKTVVF D+PK+ TGKI+K 
Sbjct: 481 ETLGESMCAFVKLKEGAEAREEEIIEFCKRKLGNKNMKMIPKTVVFSDVPKTPTGKIRKN 540

Query: 541 ILKEEAKAMDSLTKRVTK 556
           +L++ AK M  +  R  +
Sbjct: 541 VLRKMAKDMGYVQLRAVE 546

BLAST of Bhi09G002411 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 1.6e-217
Identity = 355/552 (64.31%), Postives = 448/552 (81.16%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           MEGT +  AN VPLTPI+FL+R+A V+ DR+S+VYG+V++TWR+T +RC ++ASAL QLG
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           IS  DVV+VLAPNVPA  ELHF VPMA A+LCTLN+RHD+++V+ LL HS  K+I  D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 FLSIVKAAIKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDFK 180
           FL I + A +I+S    K +P +V++ E    + +++  + +   +EYE ++  GK DF+
Sbjct: 123 FLQIAEGACEILSNKGDK-VPILVLIPE--PLTQSVSRKKRSEEMMEYEDVVAMGKSDFE 182

Query: 181 IKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMFH 240
           +  P DE D IS+NYTSGTTS PKGV+YSHRG YLN+L+ +LLN+M S P YLWT PMFH
Sbjct: 183 VIRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFH 242

Query: 241 CNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQKS 300
           CNGW L WGV A GGTNIC RNVTAK IF NIS HKVTHMGGAPT+LNMIINAP +EQK 
Sbjct: 243 CNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKP 302

Query: 301 LPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQA 360
           LP KV+ +TG APPP+HV++KM E GF + H YGLTET G  T+C WKPEWD+LP ++QA
Sbjct: 303 LPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQA 362

Query: 361 KLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAFN 420
           K+K+RQG+ H+GLE +++KDP+TM ++PADG TMGEV+FRGNTVM+GY K+ +AT+EAF 
Sbjct: 363 KMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFK 422

Query: 421 GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRPD 480
           GGWF SGDLGV+HPDGYIELKDRSKDIIISGGENISSIEVES LF HP VLEAA+V RPD
Sbjct: 423 GGWFWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPD 482

Query: 481 DHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGKIQKLILK 540
           ++WGET CAFV LKDG  A+ EE+I +CRD LPHYMAP+++VF+DLPK+STGK+QK +L+
Sbjct: 483 EYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLR 542

Query: 541 EEAKAMDSLTKR 553
            +AKA+ SL+K+
Sbjct: 543 TKAKALVSLSKK 551

BLAST of Bhi09G002411 vs. ExPASy Swiss-Prot
Match: Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 2.0e-207
Identity = 348/556 (62.59%), Postives = 435/556 (78.24%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           +EG  R  AN  PL+PITFLER+A V+ DR SLV+G+V+ TW +T +RC +LASAL  LG
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLG 107

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           ISR DVVA LAPNVPA +ELHFAVPMA  +LC LN R D + +S LL+HSEAKI+ VD+Q
Sbjct: 108 ISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQ 167

Query: 121 FLSIVKAAIKIMSKT--TTKELPRIVIVKEFDHPSSNINGFESASN----DLEYESLLDT 180
            L I   A+ +++K+  T K L  ++I +  D   S+ +   + ++    D EYE+LL +
Sbjct: 168 LLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKS 227

Query: 181 GKLDFKIKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLW 240
           G  +F+I  P+ EWDPIS+NYTSGTTSRPKGV+YSHRG YLN+L+T+ L+ MS  PVYLW
Sbjct: 228 GDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVYLW 287

Query: 241 TVPMFHCNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAP 300
           TVPMFHCNGW L WGVAAQGGTNIC R V+ K IF NI++HKVTHMGGAPTVLNMI+N  
Sbjct: 288 TVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYT 347

Query: 301 INEQKSLPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDAL 360
           + E K LP +V +MTGG+PP   +L KM E GF + H YGLTET G  T C WKPEWD+L
Sbjct: 348 VTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSL 407

Query: 361 PDDKQAKLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKA 420
             +++ KLK+RQG+QH+GLEG+++KDP+TME+VP DG TMGEVMFRGNTVMSGYFKD +A
Sbjct: 408 SLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEA 467

Query: 421 TQEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAA 480
           T++AF G WF SGDL V++PDGYIE+KDR KD+IISGGENISS+EVE VL +H +VLEAA
Sbjct: 468 TRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLEAA 527

Query: 481 IVGRPDDHWGETPCAFVNLKDGFNATE-EEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGK 540
           +V RPD HWG+TPC FV LK+GF+  + EEII FCRD+LPHYMAPKT+VF D+PK+STGK
Sbjct: 528 VVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTSTGK 587

Query: 541 IQKLILKEEAKAMDSL 550
           +QK +L+++A  M SL
Sbjct: 588 VQKYLLRKKADEMGSL 603

BLAST of Bhi09G002411 vs. ExPASy Swiss-Prot
Match: M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 5.5e-202
Identity = 345/554 (62.27%), Postives = 428/554 (77.26%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASAL-VQL 60
           MEG  +CSAN VPL+PITFLER++  + D  SLVYG+VR+TW +T  RC KLASAL   L
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHL 88

Query: 61  GISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDY 120
           GIS  DVVA  + N+P  YELHFAVPMA  +LCTLN R+D+ MVSTLL+HSEAK+I V+ 
Sbjct: 89  GISPGDVVATFSYNLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148

Query: 121 QFLSIVKAAIKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDF 180
           Q L   +AA+ ++++   K  P +V++ + +        F S+S D  Y  LL  G  DF
Sbjct: 149 QLLETARAALDLLAQKDIKP-PTLVLLTDSE-------SFTSSSYD-HYNHLLANGSDDF 208

Query: 181 KIKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           +I+ PK+EWDPIS+NYTSGTT+RPK V+YSHRG YLN+++T+LL+ M +  VYLW+VPMF
Sbjct: 209 EIRRPKNEWDPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTTSVYLWSVPMF 268

Query: 241 HCNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPI-NEQ 300
           HCNGW   WG AAQG TNIC R V+ K IF NI LHKVTH G APTVLNMI+N+P  N  
Sbjct: 269 HCNGWCFPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLH 328

Query: 301 KSLPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDK 360
             LP KV VMTGG+PPP  V+ +M E GF + H YGLTETCG A  C  KPEWDAL  ++
Sbjct: 329 TPLPHKVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETCGPAANCVCKPEWDALQPEE 388

Query: 361 QAKLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEA 420
           +  LK+RQG+ H+ +E ++++DP+TMESV ADG T+GEVMFRGNTVMSGYFKD KAT+EA
Sbjct: 389 RYALKARQGLNHLAMEEMDVRDPVTMESVRADGATIGEVMFRGNTVMSGYFKDLKATEEA 448

Query: 421 FNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGR 480
           F GGWFRSGDLGV+H DGYI+LKDR KD++ISGGENIS++EVE+VL++H +VLEAA+V R
Sbjct: 449 FEGGWFRSGDLGVKHEDGYIQLKDRKKDVVISGGENISTVEVETVLYSHEAVLEAAVVAR 508

Query: 481 PDDHWGETPCAFVNLKDGF--NATEEEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGKIQK 540
           PD  WGETPCAFV LK+GF  + + ++IIKFCRD LPHYMAPKTVVF++LPK+STGKIQK
Sbjct: 509 PDKLWGETPCAFVTLKEGFDNDVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQK 568

Query: 541 LILKEEAKAMDSLT 551
            ILKE+A AM SL+
Sbjct: 569 YILKEKAMAMGSLS 573

BLAST of Bhi09G002411 vs. ExPASy Swiss-Prot
Match: M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 3.3e-199
Identity = 341/554 (61.55%), Postives = 427/554 (77.08%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASAL-VQL 60
           MEG  +CSAN VPL+PITFLER++  + D  SLVYG+VR+TW +T  RC KLASAL    
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHF 88

Query: 61  GISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDY 120
           GIS  DVVA  + N+P  YELHFAVPMA  +LCTLN R+D+ MVSTLL+HSEAK+I V+ 
Sbjct: 89  GISPGDVVATFSYNIPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148

Query: 121 QFLSIVKAAIKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDF 180
           Q L   +AA+ ++++   K  P +V++ + +        F S+S D  Y  LL  G  DF
Sbjct: 149 QLLETARAALDLLAQKDIKP-PTLVLLTDSE-------SFTSSSYD-HYNHLLANGSDDF 208

Query: 181 KIKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           +I+ PK+E DPIS+NYTSGTT+RPK V+YSHRG YLN+++T+LL+ M +  VYLW+VPMF
Sbjct: 209 EIRRPKNECDPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTRSVYLWSVPMF 268

Query: 241 HCNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPI-NEQ 300
           HCNGW   WG AAQG TNIC R V+ K IF NI LHKVTH G APTVLNMI+N+P  N  
Sbjct: 269 HCNGWCFPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLH 328

Query: 301 KSLPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDK 360
             LP KV VMTGG+PPP  V+ +M E GF + H YGLTET G AT C  KPEWDAL  ++
Sbjct: 329 TPLPHKVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETHGPATNCVCKPEWDALQPEE 388

Query: 361 QAKLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEA 420
           +  LK+RQG+ H+ +E ++++DP++MESV ADG T+GEVMFRGNTVMSGYFKD KAT+EA
Sbjct: 389 RYALKARQGLNHLAMEEMDVRDPVSMESVRADGTTIGEVMFRGNTVMSGYFKDLKATEEA 448

Query: 421 FNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGR 480
           F GGWFR+GDLGV+H DGYI+LKDR KD++ISGGEN+S++EVE+VL++H +VLEAA+V R
Sbjct: 449 FEGGWFRTGDLGVKHEDGYIQLKDRKKDVVISGGENVSTVEVETVLYSHEAVLEAAVVAR 508

Query: 481 PDDHWGETPCAFVNLKDGFN--ATEEEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGKIQK 540
           PD  WGETPCAFV LK+GF+   + ++IIKFCRD LPHYMAPKTVVF++LPK+STGKIQK
Sbjct: 509 PDKLWGETPCAFVTLKEGFDNGVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQK 568

Query: 541 LILKEEAKAMDSLT 551
            ILKE+AKAM SL+
Sbjct: 569 YILKEKAKAMGSLS 573

BLAST of Bhi09G002411 vs. ExPASy Swiss-Prot
Match: I3PB36 (Trans-cinnamate:CoA ligase, peroxisomal OS=Petunia hybrida OX=4102 GN=CNL PE=1 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 1.0e-168
Identity = 285/555 (51.35%), Postives = 391/555 (70.45%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           M+   +C AN VPLTP+TFL RA   + +R S++Y   RFTW +T +RC +LAS+L  L 
Sbjct: 1   MDELPKCGANYVPLTPLTFLTRAFKSYANRTSIIYAGARFTWEQTYKRCCRLASSLQSLN 60

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           I + DVV+VLAPNVPATYE+HFAVPMA AVL T+N R D   ++ +L HSEAK++ VDY+
Sbjct: 61  IVKNDVVSVLAPNVPATYEMHFAVPMAGAVLNTINTRLDPMNIAIILKHSEAKLLFVDYE 120

Query: 121 FLSIVKAAIKIMSKTT--------TKELPRIVIVKEFDHPSSNINGFESASNDLEYESLL 180
           +L   + A++++  T            +P+++++ +   P+          + LEYE L+
Sbjct: 121 YLEKARKALELLMSTNFITAQNSKKISMPQVILIDDLYSPTR-----IQQQDQLEYEQLV 180

Query: 181 DTGKLDFKIKH-PKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPV 240
             G  ++  ++   DEWDPI LNYTSGTTS PKGV+YSHRG +L+TL+TI+  +M + PV
Sbjct: 181 HQGNPEYAPENIVDDEWDPIVLNYTSGTTSEPKGVVYSHRGAFLSTLNTIMGWEMGTEPV 240

Query: 241 YLWTVPMFHCNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMII 300
           YLW++PMFH NGW+LTWG+AA+GGTN+C RN TA+EI++NI+LHKVTHM  APTV N+++
Sbjct: 241 YLWSLPMFHINGWTLTWGIAARGGTNVCIRNTTAQEIYSNITLHKVTHMCCAPTVFNILL 300

Query: 301 NAPINEQKSLPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEW 360
            A  +E++ +   V VM GGAPPP+ ++ K+ E GF +VHCYG+TE  GT  VC W+ EW
Sbjct: 301 EAKPHERREITTPVQVMVGGAPPPTTLIGKIEELGFHVVHCYGITEAGGTTLVCEWQSEW 360

Query: 361 DALPDDKQAKLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKD 420
           + L  + QA LK+RQG+  + LE V++K+  TM+SVP +GKTMGE+  RG+++M GYFK+
Sbjct: 361 NKLSREDQANLKARQGISVLALEDVDVKNSKTMQSVPHNGKTMGEICLRGSSIMKGYFKN 420

Query: 421 FKATQEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVL 480
            KA  + F  GWF +GD+ V H DGY+E+KDR KDIIISGGENISSIEVE+ +  HPSV+
Sbjct: 421 DKANSQVFKNGWFLTGDVAVIHQDGYLEIKDRCKDIIISGGENISSIEVENAILKHPSVI 480

Query: 481 EAAIVGRPDDHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVF-KDLPKSS 540
           EAA+V  P   WGETPCAFV         E +II  C+  LP +M PK V F ++LPK+ 
Sbjct: 481 EAAVVAMPHPRWGETPCAFVIKTKNPEIKEADIIVHCKKELPGFMVPKHVQFLEELPKTG 540

Query: 541 TGKIQKLILKEEAKA 546
           TGK++KL L+E AK+
Sbjct: 541 TGKVKKLQLREMAKS 550

BLAST of Bhi09G002411 vs. ExPASy TrEMBL
Match: A0A5D3D8V2 (Putative acyl-activating enzyme 1, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00710 PE=4 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 5.2e-288
Identity = 488/552 (88.41%), Postives = 517/552 (93.66%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           MEG   CSAN +PLTPITFLERAAAVF DRISLVYGNVRFTW ETL+RCTK ASALV LG
Sbjct: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           ISR DVVAVLAPNVPATYELHFAVPMA A+LCTLNMRHDA M+STLL HSEAKII+VD+Q
Sbjct: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120

Query: 121 FLSIVKAAIKIMSKTT-TKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDF 180
           +L IVKAAI+IMSK T  +ELPRIVIV+EFDHPSSNINGF+SAS+DLEYESLL+TG LDF
Sbjct: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180

Query: 181 KIKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           +I+ P DEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF
Sbjct: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240

Query: 241 HCNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQK 300
           HCNGWS+TWGVAAQGGTNICQRNV AKEIF NISLHKVTHMGGAPTVLNMI+NAPI EQK
Sbjct: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300

Query: 301 SLPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQ 360
            LP KVTVMTGGAPPPS++LYKMRE GFLIVHCYGLTETCG AT C WKPEWD+LPDDKQ
Sbjct: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360

Query: 361 AKLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAF 420
           AKLKSRQGMQHIG+EG++IK+PITMESVPADGKTMGEVM RGNTVMSGY KD KAT+EAF
Sbjct: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRP 480
           NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENIS+IEVESVLFNHPSVLEAAIVGRP
Sbjct: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480

Query: 481 DDHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFK-DLPKSSTGKIQKLI 540
           DDHWGETPCAFVNLKDG NAT+EEII+FCRD LPHYMAPKTVVFK DLPKSSTGKIQKLI
Sbjct: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540

Query: 541 LKEEAKAMDSLT 551
           LKEEAKAM SL+
Sbjct: 541 LKEEAKAMGSLS 552

BLAST of Bhi09G002411 vs. ExPASy TrEMBL
Match: A0A1S3BV31 (probable acyl-activating enzyme 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103493806 PE=4 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 5.2e-288
Identity = 488/552 (88.41%), Postives = 517/552 (93.66%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           MEG   CSAN +PLTPITFLERAAAVF DRISLVYGNVRFTW ETL+RCTK ASALV LG
Sbjct: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           ISR DVVAVLAPNVPATYELHFAVPMA A+LCTLNMRHDA M+STLL HSEAKII+VD+Q
Sbjct: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120

Query: 121 FLSIVKAAIKIMSKTT-TKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDF 180
           +L IVKAAI+IMSK T  +ELPRIVIV+EFDHPSSNINGF+SAS+DLEYESLL+TG LDF
Sbjct: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180

Query: 181 KIKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           +I+ P DEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF
Sbjct: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240

Query: 241 HCNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQK 300
           HCNGWS+TWGVAAQGGTNICQRNV AKEIF NISLHKVTHMGGAPTVLNMI+NAPI EQK
Sbjct: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300

Query: 301 SLPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQ 360
            LP KVTVMTGGAPPPS++LYKMRE GFLIVHCYGLTETCG AT C WKPEWD+LPDDKQ
Sbjct: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360

Query: 361 AKLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAF 420
           AKLKSRQGMQHIG+EG++IK+PITMESVPADGKTMGEVM RGNTVMSGY KD KAT+EAF
Sbjct: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRP 480
           NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENIS+IEVESVLFNHPSVLEAAIVGRP
Sbjct: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480

Query: 481 DDHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFK-DLPKSSTGKIQKLI 540
           DDHWGETPCAFVNLKDG NAT+EEII+FCRD LPHYMAPKTVVFK DLPKSSTGKIQKLI
Sbjct: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540

Query: 541 LKEEAKAMDSLT 551
           LKEEAKAM SL+
Sbjct: 541 LKEEAKAMGSLS 552

BLAST of Bhi09G002411 vs. ExPASy TrEMBL
Match: A0A5A7VBM0 (Putative acyl-activating enzyme 1, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003580 PE=4 SV=1)

HSP 1 Score: 993.0 bits (2566), Expect = 4.9e-286
Identity = 484/546 (88.64%), Postives = 512/546 (93.77%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           MEG   CSAN +PLTPITFLERAAAVF DRISLVYGNVRFTW ETL+RCTK ASALV LG
Sbjct: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           ISR DVVAVLAPNVPATYELHFAVPMA A+LCTLNMRHDA M+STLL HSEAKII+VD+Q
Sbjct: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120

Query: 121 FLSIVKAAIKIMSKTT-TKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDF 180
           +L IVKAAI+IMSK T  +ELPRIVIV+EFDHPSSNINGF+SAS+DLEYESLL+TG LDF
Sbjct: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180

Query: 181 KIKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           +I+ P DEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF
Sbjct: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240

Query: 241 HCNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQK 300
           HCNGWS+TWGVAAQGGTNICQRNV AKEIF NISLHKVTHMGGAPTVLNMI+NAPI EQK
Sbjct: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300

Query: 301 SLPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQ 360
            LP KVTVMTGGAPPPS++LYKMRE GFLIVHCYGLTETCG AT C WKPEWD+LPDDKQ
Sbjct: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360

Query: 361 AKLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAF 420
           AKLKSRQGMQHIG+EG++IK+PITMESVPADGKTMGEVM RGNTVMSGY KD KAT+EAF
Sbjct: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRP 480
           NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENIS+IEVESVLFNHPSVLEAAIVGRP
Sbjct: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480

Query: 481 DDHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFK-DLPKSSTGKIQKLI 540
           DDHWGETPCAFVNLKDG NAT+EEII+FCRD LPHYMAPKTVVFK DLPKSSTGKIQKLI
Sbjct: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540

Query: 541 LKEEAK 545
           LKEEAK
Sbjct: 541 LKEEAK 546

BLAST of Bhi09G002411 vs. ExPASy TrEMBL
Match: A0A0A0L3B4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G658540 PE=4 SV=1)

HSP 1 Score: 984.9 bits (2545), Expect = 1.3e-283
Identity = 486/552 (88.04%), Postives = 514/552 (93.12%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           MEG  RCSAN VPLTPITFLERAAAVFGDRISLVYGNVRFTWRETL+RCTK ASALV +G
Sbjct: 1   MEGI-RCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVG 60

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           ISR DVVAVLAPNVPATYELHFAVPMA A+LCTLNMRHDA MVSTLL HSEAKII+ D+Q
Sbjct: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQ 120

Query: 121 FLSIVKAAIKIMSKT-TTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDF 180
           +L IVKAAI+IMSKT   +ELPRIVIV+EFDHPSSNINGF+SAS+DLEYESLL+TG LDF
Sbjct: 121 YLHIVKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180

Query: 181 KIKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           +I+ P DE DPISLNYTSGTTSRPKGVIYSHRGTYLNTLST+LLNDMSSM VYLWTVPMF
Sbjct: 181 EIRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMF 240

Query: 241 HCNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQK 300
           HCNGWS+TWGVAAQGGTNICQRNV AKEIF NISLH VTHMGGAPTVLNMI+NAPI EQK
Sbjct: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQK 300

Query: 301 SLPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQ 360
            L  KVTVMTGGAPPPS++LYKMRE GFLIVHCYGLTET G AT CHWKPEWD+LPD+KQ
Sbjct: 301 PLLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQ 360

Query: 361 AKLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAF 420
           AKLKSRQGMQHIG+EGV+IK+PITMESVPADGKTMGEVM RGNTVM GY KD KAT+EAF
Sbjct: 361 AKLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRP 480
           NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENIS+IEVESVLFNHPSVLEAAIVGRP
Sbjct: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480

Query: 481 DDHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFK-DLPKSSTGKIQKLI 540
           DDHWGETPCAFVNLK G NATEEEIIKFCRDNLPHYMAPKTVVFK DLPKSSTGKIQKLI
Sbjct: 481 DDHWGETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540

Query: 541 LKEEAKAMDSLT 551
           LKEEAKAM +L+
Sbjct: 541 LKEEAKAMGTLS 551

BLAST of Bhi09G002411 vs. ExPASy TrEMBL
Match: A0A6J1C834 (probable acyl-activating enzyme 1, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111009236 PE=4 SV=1)

HSP 1 Score: 947.2 bits (2447), Expect = 3.1e-272
Identity = 454/551 (82.40%), Postives = 499/551 (90.56%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           MEG +RCSANCV LTPITFLER+AAV+GDR SLVYG VR+TWR+TL+RCT+LASALV+ G
Sbjct: 1   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 60

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           ISR DVVA LAPN+PA YELHFAVPMA AVLCTLNMRHDA MVSTLLSHSEAKII VDYQ
Sbjct: 61  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 120

Query: 121 FLSIVKAAIKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDFK 180
           FL +VK AIK+MSKTT K LP +V+++E+D PSS ING +SA  DLE+ESLL TG++DFK
Sbjct: 121 FLPMVKRAIKLMSKTTEK-LPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVDFK 180

Query: 181 IKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMFH 240
           I+ PKDEWDPISLNYTSGTTSRPKGVIYSHRG YLNTLST+LLNDM S+PVYLWTVPMFH
Sbjct: 181 IRRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPMFH 240

Query: 241 CNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQKS 300
           CNGW  +WGVAAQGGTNICQR VTAKEIF NIS H VTHMGGAPTVLNMIINAPI+EQK 
Sbjct: 241 CNGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQKP 300

Query: 301 LPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQA 360
           LP KVTVMTGGAPPPSHVLYKMR  GFLI+H YGLTET G ATVC WKPEWD+LP+D+QA
Sbjct: 301 LPGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQQA 360

Query: 361 KLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAFN 420
           KL SRQG+QH+GLEGV+IKDP+TMES PADGKTMGEVMFRGNTVMSGY KD KATQEAFN
Sbjct: 361 KLNSRQGLQHVGLEGVDIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEAFN 420

Query: 421 GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRPD 480
           GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVL++HPS+LE AIVGRPD
Sbjct: 421 GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGRPD 480

Query: 481 DHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGKIQKLILK 540
           DHWGETPCAFV LKDG +A  EEIIKFCR++LPHYMAP++VVF+DLPK+STGKIQK +LK
Sbjct: 481 DHWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFVLK 540

Query: 541 EEAKAMDSLTK 552
           EEA AM SL +
Sbjct: 541 EEATAMGSLQR 550