Bhi09G001396 (gene) Wax gourd (B227) v1

Overview
NameBhi09G001396
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionmitogen-activated protein kinase kinase kinase 2
Locationchr9: 44859582 .. 44861016 (-)
RNA-Seq ExpressionBhi09G001396
SyntenyBhi09G001396
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAATTGTTAAAGATTCGAAGAAGACGCCAATTCATTACCATTGAAAAAGACAGCGTTGGCGTTGGATGTATAAATTCACGATCCCTTCTTCTCTTCTCCACAAAATTCATTTCTTCTTCTTTCTCAGCCATGGACTGGACCAGAGGCCCCGCCGTCGGCCGCGGCTCCTCCGCCACCGTCTATTTGGCCACAGCCTCCTCCTCCGGCCACCAATTCGCTGTCAAATCCACCGACCTCTCTTCATCGGCCCTATTGCAGAAGGAACACGCTCTTCTCTCCAATTTCAGCTCCCCATACCTAATCAAGTGCCTAGGTTTCGATATCGAAACTGAAGCCTTTAATCCGCCTGTGTTCAATCTGTTCATGGAGTATTTGCCGGAGGGGACGTTATGGGATGCGATTCAGAGCAACGGCGGCCGGATGGAGGAATCGACGATCGGAGTATTTTCGCGTCAGATTCTCAAGGGATTGGAGTACCTTCATGCTAATGGATTGGCACATTGCGATATCAAGAGCCGGAATCTGTTACTGAGTGGTGAGGGTTTGAAAATCGCCGATCTAGGTTGTGCGAAATTTGTCGAGGGAATTTCCGGTAACAGAATTGGGACTTTTTCTGGTTCGCCGGCGTTCATGGCGCCGGAAGTTGCGAGAGGTGAAGAACAGGGGTTTCCGGCGGATGTTTGGGCTTTTGGATGTACAATCATTGAAATGGCTACTGGCGATCATCCATGGCCGGAGATGGAGGATCCGATCTCGGCGTTGTATCGGATTGGATTTTCCGACGAGTTACCGGAGATTCCGCGGTGGTTGTCGAAGAGTGCGAGGGATTTTGTCGCCAAGTGTTTGATAAAAGACCCGAAAGAAAGATGGACTGTGAAACAGCTTCTTGAGCATCCGTTTCTTCAGGGATTTGATTCTGAATCGGAGATTGAATGCTGGAGGATGAAAACGATGAGGAATTCTTCTCCGAGCTGTGTTTTAGATCAGAGCTTTTGGGATTCAATGGAGGATTTGGAGACTCCATTGAAGGTGACCCATCGAGGTTCTTTGGCGGATGGTCCGGCTGAGAGGATTAGAAGTTTGACTGGTAATTTGTCTTCTGATTGGGATTTGGAATGGGATGATCGAGATTGGGTTTTGATCAGAAACAATGGCGTCGGCGGCGGAAGCCATGGAAATGGATCTGTGAGCTCGGATTCTTCTGTTACAGTTGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAATTTTGAACTTAGAATTTGAAGAAAATTCTGTATTGTTCAGTTTAGTTAATGATGATTTTAGTATTGTGAACAGTAGGAATGCAGAATTAGAAATGGAAATTAAGAGTGATTTTATGTTGGAAACTGTGAGATTTGGAGTTAAAAAGTATTGTTCTTCTTCTTCATTAATCCAAATTCATCTCTTATTCC

mRNA sequence

GAATTGTTAAAGATTCGAAGAAGACGCCAATTCATTACCATTGAAAAAGACAGCGTTGGCGTTGGATGTATAAATTCACGATCCCTTCTTCTCTTCTCCACAAAATTCATTTCTTCTTCTTTCTCAGCCATGGACTGGACCAGAGGCCCCGCCGTCGGCCGCGGCTCCTCCGCCACCGTCTATTTGGCCACAGCCTCCTCCTCCGGCCACCAATTCGCTGTCAAATCCACCGACCTCTCTTCATCGGCCCTATTGCAGAAGGAACACGCTCTTCTCTCCAATTTCAGCTCCCCATACCTAATCAAGTGCCTAGGTTTCGATATCGAAACTGAAGCCTTTAATCCGCCTGTGTTCAATCTGTTCATGGAGTATTTGCCGGAGGGGACGTTATGGGATGCGATTCAGAGCAACGGCGGCCGGATGGAGGAATCGACGATCGGAGTATTTTCGCGTCAGATTCTCAAGGGATTGGAGTACCTTCATGCTAATGGATTGGCACATTGCGATATCAAGAGCCGGAATCTGTTACTGAGTGGTGAGGGTTTGAAAATCGCCGATCTAGGTTGTGCGAAATTTGTCGAGGGAATTTCCGGTAACAGAATTGGGACTTTTTCTGGTTCGCCGGCGTTCATGGCGCCGGAAGTTGCGAGAGGTGAAGAACAGGGGTTTCCGGCGGATGTTTGGGCTTTTGGATGTACAATCATTGAAATGGCTACTGGCGATCATCCATGGCCGGAGATGGAGGATCCGATCTCGGCGTTGTATCGGATTGGATTTTCCGACGAGTTACCGGAGATTCCGCGGTGGTTGTCGAAGAGTGCGAGGGATTTTGTCGCCAAGTGTTTGATAAAAGACCCGAAAGAAAGATGGACTGTGAAACAGCTTCTTGAGCATCCGTTTCTTCAGGGATTTGATTCTGAATCGGAGATTGAATGCTGGAGGATGAAAACGATGAGGAATTCTTCTCCGAGCTGTGTTTTAGATCAGAGCTTTTGGGATTCAATGGAGGATTTGGAGACTCCATTGAAGGTGACCCATCGAGGTTCTTTGGCGGATGGTCCGGCTGAGAGGATTAGAAGTTTGACTGGTAATTTGTCTTCTGATTGGGATTTGGAATGGGATGATCGAGATTGGGTTTTGATCAGAAACAATGGCGTCGGCGGCGGAAGCCATGGAAATGGATCTGTGAGCTCGGATTCTTCTGTTACAGTTGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAATTTTGAACTTAGAATTTGAAGAAAATTCTGTATTGTTCAGTTTAGTTAATGATGATTTTAGTATTGTGAACAGTAGGAATGCAGAATTAGAAATGGAAATTAAGAGTGATTTTATGTTGGAAACTGTGAGATTTGGAGTTAAAAAGTATTGTTCTTCTTCTTCATTAATCCAAATTCATCTCTTATTCC

Coding sequence (CDS)

ATGGACTGGACCAGAGGCCCCGCCGTCGGCCGCGGCTCCTCCGCCACCGTCTATTTGGCCACAGCCTCCTCCTCCGGCCACCAATTCGCTGTCAAATCCACCGACCTCTCTTCATCGGCCCTATTGCAGAAGGAACACGCTCTTCTCTCCAATTTCAGCTCCCCATACCTAATCAAGTGCCTAGGTTTCGATATCGAAACTGAAGCCTTTAATCCGCCTGTGTTCAATCTGTTCATGGAGTATTTGCCGGAGGGGACGTTATGGGATGCGATTCAGAGCAACGGCGGCCGGATGGAGGAATCGACGATCGGAGTATTTTCGCGTCAGATTCTCAAGGGATTGGAGTACCTTCATGCTAATGGATTGGCACATTGCGATATCAAGAGCCGGAATCTGTTACTGAGTGGTGAGGGTTTGAAAATCGCCGATCTAGGTTGTGCGAAATTTGTCGAGGGAATTTCCGGTAACAGAATTGGGACTTTTTCTGGTTCGCCGGCGTTCATGGCGCCGGAAGTTGCGAGAGGTGAAGAACAGGGGTTTCCGGCGGATGTTTGGGCTTTTGGATGTACAATCATTGAAATGGCTACTGGCGATCATCCATGGCCGGAGATGGAGGATCCGATCTCGGCGTTGTATCGGATTGGATTTTCCGACGAGTTACCGGAGATTCCGCGGTGGTTGTCGAAGAGTGCGAGGGATTTTGTCGCCAAGTGTTTGATAAAAGACCCGAAAGAAAGATGGACTGTGAAACAGCTTCTTGAGCATCCGTTTCTTCAGGGATTTGATTCTGAATCGGAGATTGAATGCTGGAGGATGAAAACGATGAGGAATTCTTCTCCGAGCTGTGTTTTAGATCAGAGCTTTTGGGATTCAATGGAGGATTTGGAGACTCCATTGAAGGTGACCCATCGAGGTTCTTTGGCGGATGGTCCGGCTGAGAGGATTAGAAGTTTGACTGGTAATTTGTCTTCTGATTGGGATTTGGAATGGGATGATCGAGATTGGGTTTTGATCAGAAACAATGGCGTCGGCGGCGGAAGCCATGGAAATGGATCTGTGAGCTCGGATTCTTCTGTTACAGTTGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAATTTTGAACTTAGAATTTGAAGAAAATTCTGTATTGTTCAGTTTAGTTAATGATGATTTTAGTATTGTGAACAGTAGGAATGCAGAATTAGAAATGGAAATTAAGAGTGATTTTATGTTGGAAACTGTGAGATTTGGAGTTAAAAAGTATTGTTCTTCTTCTTCATTAATCCAAATTCATCTCTTATTCC

Protein sequence

MDWTRGPAVGRGSSATVYLATASSSGHQFAVKSTDLSSSALLQKEHALLSNFSSPYLIKCLGFDIETEAFNPPVFNLFMEYLPEGTLWDAIQSNGGRMEESTIGVFSRQILKGLEYLHANGLAHCDIKSRNLLLSGEGLKIADLGCAKFVEGISGNRIGTFSGSPAFMAPEVARGEEQGFPADVWAFGCTIIEMATGDHPWPEMEDPISALYRIGFSDELPEIPRWLSKSARDFVAKCLIKDPKERWTVKQLLEHPFLQGFDSESEIECWRMKTMRNSSPSCVLDQSFWDSMEDLETPLKVTHRGSLADGPAERIRSLTGNLSSDWDLEWDDRDWVLIRNNGVGGGSHGNGSVSSDSSVTVEEEEEEEEEEEILNLEFEENSVLFSLVNDDFSIVNSRNAELEMEIKSDFMLETVRFGVKKYCSSSSLIQIHLLF
Homology
BLAST of Bhi09G001396 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 347.1 bits (889), Expect = 2.1e-95
Identity = 192/401 (47.88%), Postives = 258/401 (64.34%), Query Frame = 0

Query: 1   MDWTRGPAVGRGSSATVYLATASSSGHQFAVKSTDLSSSALLQKEHALLSNFSSPYLIKC 60
           ++WTRGP +GRGS+ATV +A  SSSG  FAVKS DLSSS+LLQKE ++LS  SSP+++K 
Sbjct: 3   INWTRGPIIGRGSTATVSIA-ISSSGELFAVKSADLSSSSLLQKEQSILSTLSSPHMVKY 62

Query: 61  LGFDIETEAFNPPVFNLFMEYLPEGTLWDAIQSNGGRMEESTIGVFSRQILKGLEYLHAN 120
           +G  +  E+ N  V+N+ MEY+  G L D I+++GG++ E  I  ++RQIL GL YLH  
Sbjct: 63  IGTGLTRES-NGLVYNILMEYVSGGNLHDLIKNSGGKLPEPEIRSYTRQILNGLVYLHER 122

Query: 121 GLAHCDIKSRNLLLSGEG-LKIADLGCAKFVEGISGNRIGTFSGSPAFMAPEVARGEEQG 180
           G+ HCD+KS N+L+   G LKIAD+GCAK V+         FSG+PAFMAPEVARGEEQ 
Sbjct: 123 GIVHCDLKSHNVLVEENGVLKIADMGCAKSVDK------SEFSGTPAFMAPEVARGEEQR 182

Query: 181 FPADVWAFGCTIIEMATGDHPWPEMEDPISALYRIGFSDELPEIPRWLSKSARDFVAKCL 240
           FPADVWA GCT+IEM TG  PWPE+ D ++A+Y+IGFS E P IP W+S  A+DF+  CL
Sbjct: 183 FPADVWALGCTMIEMMTGSSPWPELNDVVAAMYKIGFSGESPAIPAWISDKAKDFLKNCL 242

Query: 241 IKDPKERWTVKQLLEHPFLQGFDSESEIECWRMKTMRNSSPSCVLDQSFWDSMEDLETPL 300
            +D K+RWTV++LL+HPFL   +     +C + KT   SSPS VLDQ FWDS E  ++ L
Sbjct: 243 KEDQKQRWTVEELLKHPFLDDDEESQTSDCLKNKT---SSPSTVLDQRFWDSCESSKSHL 302

Query: 301 -KVTHRGSLA------DGPAERIRSLTGNLSS---DWDLEWDDRDWVLIRNNGVGGGSHG 360
             + H    A      D PA+RI  L G+  S   DWD E DD  W+ +R          
Sbjct: 303 VSIDHEDPFAEYSESLDSPADRIEKLAGDEFSSLLDWDTE-DDGGWIQVRGEKHKETEKR 362

Query: 361 NGS------VSSDSSVTVEEEEEEEEEEEILNLEFEENSVL 385
           +G        ++ SS T+EE E+    ++ L  E+  + ++
Sbjct: 363 DGDEDVICVEATSSSQTIEEVEDWISNQDSLFSEYSSDDII 391

BLAST of Bhi09G001396 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 338.2 bits (866), Expect = 9.9e-93
Identity = 180/380 (47.37%), Postives = 248/380 (65.26%), Query Frame = 0

Query: 2   DWTRGPAVGRGSSATVYLATASSSGHQFAVKSTDLSSSALLQKEHALLSNFSSPYLIKCL 61
           +W RGP +GRGS+ATV L   ++SG  FAVKS + SSSA LQ+E ++LS  SSPY++K +
Sbjct: 5   NWIRGPIIGRGSTATVSLG-ITNSGDFFAVKSAEFSSSAFLQREQSILSKLSSPYIVKYI 64

Query: 62  GFDIETEAFNPPVFNLFMEYLPEGTLWDAIQSNGGRMEESTIGVFSRQILKGLEYLHANG 121
           G ++ T+  +  ++NL MEY+  G+L D I+++GG++ E  I  ++RQILKGL YLH  G
Sbjct: 65  GSNV-TKENDKLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLMYLHDQG 124

Query: 122 LAHCDIKSRNLLLSGEGLKIADLGCAKFVEGISGNRIGTFSGSPAFMAPEVARGEEQGFP 181
           + HCD+KS+N+++ GE  KI DLGCAK VE    N    FSG+PAFM+PEVARGEEQ FP
Sbjct: 125 IVHCDVKSQNVMIGGEIAKIVDLGCAKTVE---ENENLEFSGTPAFMSPEVARGEEQSFP 184

Query: 182 ADVWAFGCTIIEMATGDHPWPEMEDPISALYRIGFSDELPEIPRWLSKSARDFVAKCLIK 241
           ADVWA GCT+IEMATG  PWPE+ D ++A+Y+IGF+ E P IP WLS+  +DF+ KCL K
Sbjct: 185 ADVWALGCTVIEMATGSSPWPELNDVVAAIYKIGFTGESPVIPVWLSEKGQDFLRKCLRK 244

Query: 242 DPKERWTVKQLLEHPFLQGFDSESEIECWRMKTMRNSSPSCVLDQSFWDSMEDLETP-LK 301
           DPK+RWTV++LL+HPFL   D++S+        + +SSPS VLDQ FWD  E   +  +K
Sbjct: 245 DPKQRWTVEELLQHPFLDEEDNDSD---QTGNCLNSSSPSTVLDQRFWDLCETSRSRFIK 304

Query: 302 VTHRGSLADG----------PAERIRSLTGNLSSDWDLEWDDRDWVLIRNNGVGGGSHGN 361
             H    A+           P +RI+ L G+ SS  + +W+   W+ +R     G     
Sbjct: 305 EDHEDPFANSTNFLWDDDSLPGDRIKKLAGDESSG-EPDWETNGWIEVR-----GEIEKR 364

Query: 362 GSVSSDSSVTVEEEEEEEEE 371
                ++ V     EE+EEE
Sbjct: 365 NEEEDENCVEATSLEEDEEE 370

BLAST of Bhi09G001396 vs. TAIR 10
Match: AT2G32510.1 (mitogen-activated protein kinase kinase kinase 17 )

HSP 1 Score: 292.4 bits (747), Expect = 6.2e-79
Identity = 155/353 (43.91%), Postives = 226/353 (64.02%), Query Frame = 0

Query: 1   MDWTRGPAVGRGSSATVYLATASSSGHQFAVKSTDLSSSALLQKEHALLSNFSSPYLIKC 60
           M+WTRG  +GRGS+ATVY A   +S    AVKS+++  S  LQ+E  +LS+ SSPY+I  
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDEILAVKSSEVHRSEFLQREAKILSSLSSPYVIGY 60

Query: 61  LGFDIETEAFNPPVFNLFMEYLPEGTLWDAIQSNGGRMEESTIGVFSRQILKGLEYLHAN 120
            G + + E+    ++NL MEY P GTL DA   +GGR++E+ +  ++R ILKGLEY+H+ 
Sbjct: 61  RGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYIHSK 120

Query: 121 GLAHCDIKSRNLLLSGEG-LKIADLGCAKFVEGISGNRIGTFSGSPAFMAPEVARGEEQG 180
           G+ HCD+K  N+++S +G  KIAD GCAK V+ +  + +    G+PAFMAPEVARGE+QG
Sbjct: 121 GIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESPV---MGTPAFMAPEVARGEKQG 180

Query: 181 FPADVWAFGCTIIEMATGDHPWPE---MEDPISALYRIGFSDELPEIPRWLSKSARDFVA 240
             +D+WA GCT+IEM TG  PW +    EDP+S LYR+G+S E PE+P  L++ A+DF+ 
Sbjct: 181 KESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAKDFLE 240

Query: 241 KCLIKDPKERWTVKQLLEHPFLQGFDSESEIECWRMKTMRNSSPSCVLDQSFWDSM---- 300
           KCL ++  ERWT  QLL HPFL    ++ +IE   +  + ++SP+ V DQ+FW S+    
Sbjct: 241 KCLKREANERWTATQLLNHPFL---TTKPDIEPVLVPGLISNSPTSVTDQTFWRSVEEEE 300

Query: 301 ----EDLETPLKVTHRGSLADGPAERIRSL--TGNLSSDWDLEWDDRDWVLIR 340
               E+++   +   R SL    +ERI  L   G L      + +  DW+++R
Sbjct: 301 EEETEEIQKDSRDLDRLSLWGCYSERIGRLKCVGGLDGT-RCDMEGGDWIMVR 346

BLAST of Bhi09G001396 vs. TAIR 10
Match: AT1G05100.1 (mitogen-activated protein kinase kinase kinase 18 )

HSP 1 Score: 282.0 bits (720), Expect = 8.4e-76
Identity = 160/353 (45.33%), Postives = 216/353 (61.19%), Query Frame = 0

Query: 1   MDWTRGPAVGRGSSATVYLATASSSGHQFAVKSTDLSSSALLQKEHALLSNFSSPYLIKC 60
           M+WTRG  +GRGS+ATV  AT   SG   AVKS +   S  LQ+E  +LS+ +SPY+I  
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGETLAVKSAEFHRSEFLQREAKILSSLNSPYVIGY 60

Query: 61  LGFDIETEAFN----PPVFNLFMEYLPEGTLWDAIQSNGGRMEESTIGVFSRQILKGLEY 120
            G +I  E F+       ++L MEY P GTL D    NGG ++E+ +  ++RQIL GLEY
Sbjct: 61  RGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQILLGLEY 120

Query: 121 LH-ANGLAHCDIKSRNLLLSGEG-LKIADLGCAKFVEGISGNRIGTFSGSPAFMAPEVAR 180
           +H + G+AHCDIK  N+L+   G  KIAD GCAK+VE      +    G+PAFMAPE AR
Sbjct: 121 IHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITEPV---RGTPAFMAPEAAR 180

Query: 181 GEEQGFPADVWAFGCTIIEMATGDHPW--PEMEDPISALYRIGFSDELPEIPRWLSKSAR 240
           GE QG  +D+WA GCT+IEM TG  PW   +  DP+S LYR+G+  ELPE+P  L++ A+
Sbjct: 181 GERQGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPELPCSLTEQAK 240

Query: 241 DFVAKCLIKDPKERWTVKQLLEHPFLQGFDSESEIECWRMKTMRNSSPSCVLDQSFWDSM 300
           DF+ KCL K+  ERWT  QLL HPFL   + E E+    +  +  +SP+ V DQ FW S+
Sbjct: 241 DFLGKCLKKEATERWTASQLLNHPFL--VNKEPEL----VTGLVTNSPTSVTDQMFWRSV 300

Query: 301 EDLETPLKVTHRGSLADGPAERIRSLT--GN--LSSDWDLEWDDRDWVLIRNN 342
           E+  +  + +      D   ERI  L+  G+  + S WDL  D  DW+ +R N
Sbjct: 301 EEEVSEDRSSWWECHED---ERIGVLSWIGHVVVESTWDL--DGEDWITVRRN 339

BLAST of Bhi09G001396 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 215.7 bits (548), Expect = 7.4e-56
Identity = 132/351 (37.61%), Postives = 190/351 (54.13%), Query Frame = 0

Query: 1   MDWTRGPAVGRGSSATVYLATASSSGHQ-----FAVKSTDLSSSALLQKEHALLSNFSS- 60
           M+W R   +G GS +TV LAT S S  +      AVKS+ +  SA L+ E  +L +    
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDC 60

Query: 61  PYLIKCLGFDIETEAFNPPVFNLFMEYLPEGTLWDAIQSNGGRMEESTIGVFSRQILKGL 120
             +++C G +  T      ++NLF+EY   G+L D I+S+G  + E  +  F+R I+KGL
Sbjct: 61  SEIVRCFG-EGRTVENGEEIYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIVKGL 120

Query: 121 EYLHANGLAHCDIKSRNLLLSGEG-LKIADLGCAKFVEGISGNRIGTFSGSPAFMAPEVA 180
            ++H NG  HCDIK  N+L+ G+G +KI+D G AK     SG       G+P +MAPE  
Sbjct: 121 CHIHGNGFTHCDIKLENVLVFGDGDVKISDFGLAK---RRSGEVCVEIRGTPLYMAPESV 180

Query: 181 RGEEQGFPADVWAFGCTIIEMATGDHPW----PEMEDPISALYRIGFSDELPEIPRWLSK 240
              E   PAD+WA GC+++EM++G   W      M + +S L RIG  DE+P IP  LS+
Sbjct: 181 NHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVPRIPVELSE 240

Query: 241 SARDFVAKCLIKDPKERWTVKQLLEHPFLQGFDSESEIECWRMKTMRNSSPSCVLDQSFW 300
             +DFV+KC +K+  ERWT + LL+HPFL   D   E +        + SP    D   W
Sbjct: 241 EGKDFVSKCFVKNAAERWTAEMLLDHPFLAVDDESGEED-----EACSVSPRNPFDFPGW 300

Query: 301 DSMEDLETPLKVTHR-GSLADGPAERIRSLTGNLSSDWDLEWDDRDWVLIR 340
           +S   +++P+  +   GSL   P ERI  L      DW +     DWV +R
Sbjct: 301 NS---VQSPVNDSVMFGSLVGSPEERISGLVSEKVPDWSVSC---DWVNVR 336

BLAST of Bhi09G001396 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 8.7e-78
Identity = 155/353 (43.91%), Postives = 226/353 (64.02%), Query Frame = 0

Query: 1   MDWTRGPAVGRGSSATVYLATASSSGHQFAVKSTDLSSSALLQKEHALLSNFSSPYLIKC 60
           M+WTRG  +GRGS+ATVY A   +S    AVKS+++  S  LQ+E  +LS+ SSPY+I  
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDEILAVKSSEVHRSEFLQREAKILSSLSSPYVIGY 60

Query: 61  LGFDIETEAFNPPVFNLFMEYLPEGTLWDAIQSNGGRMEESTIGVFSRQILKGLEYLHAN 120
            G + + E+    ++NL MEY P GTL DA   +GGR++E+ +  ++R ILKGLEY+H+ 
Sbjct: 61  RGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYIHSK 120

Query: 121 GLAHCDIKSRNLLLSGEG-LKIADLGCAKFVEGISGNRIGTFSGSPAFMAPEVARGEEQG 180
           G+ HCD+K  N+++S +G  KIAD GCAK V+ +  + +    G+PAFMAPEVARGE+QG
Sbjct: 121 GIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESPV---MGTPAFMAPEVARGEKQG 180

Query: 181 FPADVWAFGCTIIEMATGDHPWPE---MEDPISALYRIGFSDELPEIPRWLSKSARDFVA 240
             +D+WA GCT+IEM TG  PW +    EDP+S LYR+G+S E PE+P  L++ A+DF+ 
Sbjct: 181 KESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAKDFLE 240

Query: 241 KCLIKDPKERWTVKQLLEHPFLQGFDSESEIECWRMKTMRNSSPSCVLDQSFWDSM---- 300
           KCL ++  ERWT  QLL HPFL    ++ +IE   +  + ++SP+ V DQ+FW S+    
Sbjct: 241 KCLKREANERWTATQLLNHPFL---TTKPDIEPVLVPGLISNSPTSVTDQTFWRSVEEEE 300

Query: 301 ----EDLETPLKVTHRGSLADGPAERIRSL--TGNLSSDWDLEWDDRDWVLIR 340
               E+++   +   R SL    +ERI  L   G L      + +  DW+++R
Sbjct: 301 EEETEEIQKDSRDLDRLSLWGCYSERIGRLKCVGGLDGT-RCDMEGGDWIMVR 346

BLAST of Bhi09G001396 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 1.2e-74
Identity = 160/353 (45.33%), Postives = 216/353 (61.19%), Query Frame = 0

Query: 1   MDWTRGPAVGRGSSATVYLATASSSGHQFAVKSTDLSSSALLQKEHALLSNFSSPYLIKC 60
           M+WTRG  +GRGS+ATV  AT   SG   AVKS +   S  LQ+E  +LS+ +SPY+I  
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGETLAVKSAEFHRSEFLQREAKILSSLNSPYVIGY 60

Query: 61  LGFDIETEAFN----PPVFNLFMEYLPEGTLWDAIQSNGGRMEESTIGVFSRQILKGLEY 120
            G +I  E F+       ++L MEY P GTL D    NGG ++E+ +  ++RQIL GLEY
Sbjct: 61  RGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQILLGLEY 120

Query: 121 LH-ANGLAHCDIKSRNLLLSGEG-LKIADLGCAKFVEGISGNRIGTFSGSPAFMAPEVAR 180
           +H + G+AHCDIK  N+L+   G  KIAD GCAK+VE      +    G+PAFMAPE AR
Sbjct: 121 IHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITEPV---RGTPAFMAPEAAR 180

Query: 181 GEEQGFPADVWAFGCTIIEMATGDHPW--PEMEDPISALYRIGFSDELPEIPRWLSKSAR 240
           GE QG  +D+WA GCT+IEM TG  PW   +  DP+S LYR+G+  ELPE+P  L++ A+
Sbjct: 181 GERQGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPELPCSLTEQAK 240

Query: 241 DFVAKCLIKDPKERWTVKQLLEHPFLQGFDSESEIECWRMKTMRNSSPSCVLDQSFWDSM 300
           DF+ KCL K+  ERWT  QLL HPFL   + E E+    +  +  +SP+ V DQ FW S+
Sbjct: 241 DFLGKCLKKEATERWTASQLLNHPFL--VNKEPEL----VTGLVTNSPTSVTDQMFWRSV 300

Query: 301 EDLETPLKVTHRGSLADGPAERIRSLT--GN--LSSDWDLEWDDRDWVLIRNN 342
           E+  +  + +      D   ERI  L+  G+  + S WDL  D  DW+ +R N
Sbjct: 301 EEEVSEDRSSWWECHED---ERIGVLSWIGHVVVESTWDL--DGEDWITVRRN 339

BLAST of Bhi09G001396 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 3.3e-53
Identity = 129/354 (36.44%), Postives = 181/354 (51.13%), Query Frame = 0

Query: 1   MDWTRGPAVGRGSSATVYLATASSSGHQF----AVKSTDLSSSALLQKEHALLSNFSS-P 60
           M+W RG  +G G+ +TV  AT S +   F    AVKSTD   +A L  E ++L +    P
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCP 60

Query: 61  YLIKCLGFDIETEAFNPPVFNLFMEYLPEGTLWDAIQSNGGR-MEESTIGVFSRQILKGL 120
            +I+C G D   E     + NL +EY   G+L   ++  GG  + EST+   +  +L+GL
Sbjct: 61  EIIRCYGEDSTVEN-GEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGL 120

Query: 121 EYLHANGLAHCDIKSRNLLLSGEG-LKIADLGCAKFVEG--ISGNRIGTFSGSPAFMAPE 180
            ++HA G AHCDIK  N+LL  +G +KIAD G A  V+G   +  +     G+P +MAPE
Sbjct: 121 RHIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPE 180

Query: 181 VARGEEQGFPADVWAFGCTIIEMATGDHPWPEMEDP--ISALYRIGFSDELPEIPRWLSK 240
                E G  ADVWA GC ++EM +G   W   E    +S L RIG  DELP+IP  LS+
Sbjct: 181 CVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSE 240

Query: 241 SARDFVAKCLIKDPKERWTVKQLLEHPFLQGFDSESEIECWRMKTMRNS----SPSCVLD 300
             +DF++KC +KDP +RWT + LL H F+     +   E + +K         SP C  +
Sbjct: 241 EGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCPFE 300

Query: 301 QSFWDSMEDLETPLKVTHRGSLADGPAERIRSLTGNLSSDWDLEWDDRDWVLIR 340
              WDS      P          D P ER+ SL      DW +      W+ +R
Sbjct: 301 FDDWDSFTLDSNP--------SFDSPVERLGSLVSGSIPDWSV---GGSWLTVR 342

BLAST of Bhi09G001396 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 180.6 bits (457), Expect = 3.7e-44
Identity = 94/276 (34.06%), Postives = 155/276 (56.16%), Query Frame = 0

Query: 3   WTRGPAVGRGSSATVYLATASSSGHQFAVKS---TDLSSSALLQ-------KEHALLSNF 62
           W +G  +GRG   +VYL     +G  FAVK     D++S   L+       KE  ++ + 
Sbjct: 170 WQKGQILGRGGYGSVYLGLNKDTGELFAVKQLEIVDINSDPKLKNMILSFSKEIEVMRSL 229

Query: 63  SSPYLIKCLGFDIETEAFNPPVFNLFMEYLPEGTLWDAIQSNGGRMEESTIGVFSRQILK 122
               +++ LG  ++         ++F+EY+P G++  ++    G   E+ I V+++QIL+
Sbjct: 230 RHDNIVRYLGTSLDQS-----FLSVFLEYIPGGSI-SSLLGKFGAFSENVIKVYTKQILQ 289

Query: 123 GLEYLHANGLAHCDIKSRNLLLSGEGL-KIADLGCAKFVEGISGNRIGTFSGSPAFMAPE 182
           GL +LHAN + H DIK  N+L+  +G+ K++D GC+K   GI  ++  +  G+P +MAPE
Sbjct: 290 GLSFLHANSIIHRDIKGANILIDTKGIVKLSDFGCSKSFSGIV-SQFKSMQGTPYWMAPE 349

Query: 183 VARGEEQGFPADVWAFGCTIIEMATGDHPWPEMEDPISALYRIGFSDELPEIPRWLSKSA 242
           V +    G  +D+W+ GC I+EMAT   PW  + +  + +Y I  S+ +P IP  +S+ A
Sbjct: 350 VIKQTGHGRSSDIWSLGCVIVEMATAQPPWSNITELAAVMYHIASSNSIPNIPSHMSQEA 409

Query: 243 RDFVAKCLIKDPKERWTVKQLLEHPFLQGFDSESEI 268
            DF+  C  +DPKER    QLL+HPF+   D   ++
Sbjct: 410 FDFLNLCFKRDPKERPDANQLLKHPFIMNLDDNIQL 438

BLAST of Bhi09G001396 vs. ExPASy Swiss-Prot
Match: O22042 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=ANP3 PE=1 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 2.4e-43
Identity = 99/271 (36.53%), Postives = 152/271 (56.09%), Query Frame = 0

Query: 3   WTRGPAVGRGSSATVYLATASSSGHQFAVKSTDLSSSAL-----------LQKEHALLSN 62
           W +G  +G G+   VY+     SG   A+K   ++ S+            L++E  LL N
Sbjct: 68  WRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRELEEEVQLLKN 127

Query: 63  FSSPYLIKCLGFDIETEAFNPPVFNLFMEYLPEGTLWDAIQSNGGRMEESTIGVFSRQIL 122
            S P +++ LG   E+++      N+ ME++P G++  ++    G   E  I ++++Q+L
Sbjct: 128 LSHPNIVRYLGTVRESDS-----LNILMEFVPGGSI-SSLLEKFGSFPEPVIIMYTKQLL 187

Query: 123 KGLEYLHANGLAHCDIKSRNLLLSGEG-LKIADLGCA-KFVEGISGNRIGTFSGSPAFMA 182
            GLEYLH NG+ H DIK  N+L+  +G +++AD G + K VE  + N   +  G+P +MA
Sbjct: 188 LGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTPYWMA 247

Query: 183 PEVARGEEQGFPADVWAFGCTIIEMATGDHPWPEMEDPISALYRIGFSDELPEIPRWLSK 242
           PEV       F AD+W+ GCT+IEMATG  PW E     +A+  IG +   P IP  LS 
Sbjct: 248 PEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHPPIPEDLSP 307

Query: 243 SARDFVAKCLIKDPKERWTVKQLLEHPFLQG 261
            A+DF+ KCL K+P  R +  +LL+HPF+ G
Sbjct: 308 EAKDFLMKCLHKEPSLRLSATELLQHPFVTG 332

BLAST of Bhi09G001396 vs. ExPASy TrEMBL
Match: A0A1S3BIX8 (mitogen-activated protein kinase kinase kinase 2 OS=Cucumis melo OX=3656 GN=LOC103490380 PE=4 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 1.9e-200
Identity = 353/435 (81.15%), Postives = 383/435 (88.05%), Query Frame = 0

Query: 1   MDWTRGPAVGRGSSATVYLATASSSGHQFAVKSTDLSSSALLQKEHALLSNFSSPYLIKC 60
           MDWTRGP VGRGSSATVY+AT +SSG QFAVKST+L+SSALL KEHALLSN SSPYLIKC
Sbjct: 1   MDWTRGPPVGRGSSATVYMATLNSSGQQFAVKSTNLASSALLHKEHALLSNLSSPYLIKC 60

Query: 61  LGFDIETEAFNPPVFNLFMEYLPEGTLWDAIQSNGGRMEESTIGVFSRQILKGLEYLHAN 120
           LGFD+ET+  NPPVFNLFMEY PEGTLWDAIQ NGGR+EES IGVFSRQILKGLEYLHAN
Sbjct: 61  LGFDVETQPLNPPVFNLFMEYFPEGTLWDAIQINGGRIEESMIGVFSRQILKGLEYLHAN 120

Query: 121 GLAHCDIKSRNLLLSGEGLKIADLGCAKFVEGISGNRIGTFSGSPAFMAPEVARGEEQGF 180
           GLAHCDIKSRNLLLS EGLKIADLGCAKFV+GISGN +G FSGSPAFMAPEVARGEEQGF
Sbjct: 121 GLAHCDIKSRNLLLSDEGLKIADLGCAKFVDGISGNGVGAFSGSPAFMAPEVARGEEQGF 180

Query: 181 PADVWAFGCTIIEMATGDHPWPEMEDPISALYRIGFSDELPEIPRWLSKSARDFVAKCLI 240
           PADVWAFGCT+IEMATGDHPWPE+EDP+SALYRIGFS E+PEIPRWLS+SA+DFVAKCLI
Sbjct: 181 PADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGEVPEIPRWLSESAKDFVAKCLI 240

Query: 241 KDPKERWTVKQLLEHPFLQGFDSESEIECWRMKTMRNSSPSCVLDQSFWDSMEDLETPLK 300
           KDPKERW+VK+LLEHPFL+GFDSESEIE  RMKT+ +SSPSCVLDQ FWDSMEDLE+P K
Sbjct: 241 KDPKERWSVKRLLEHPFLEGFDSESEIEGSRMKTVNSSSPSCVLDQRFWDSMEDLESPSK 300

Query: 301 VTHRGSLADGPAERIRSLTGNLSSDWDLEWDDRDWVLIRNNGVGGGSHGNGSVSSDSSVT 360
           VT + SL D PAERIR L  NLS D +LEWDD DWVLIRNNGVGGGSHGNGSVS D   T
Sbjct: 301 VTQQASLVDCPAERIRRLADNLSCDLELEWDDGDWVLIRNNGVGGGSHGNGSVSLDFCAT 360

Query: 361 VEEEEEEEEEEEILNLEFEENSVLFSLVNDDFSIVNSRNAELEMEIKSDFMLETVRFGVK 420
           +      EEE+EILNLE EENSVLF LV+DDFS+VNSRN  LEME+KSDFMLETV     
Sbjct: 361 I------EEEDEILNLEIEENSVLFCLVDDDFSVVNSRNGGLEMELKSDFMLETV----- 420

Query: 421 KYCSSSSLIQIHLLF 436
           +   S +LIQIHLLF
Sbjct: 421 ENHFSFTLIQIHLLF 424

BLAST of Bhi09G001396 vs. ExPASy TrEMBL
Match: A0A6J1IHI8 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=3661 GN=LOC111475873 PE=3 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 1.1e-189
Identity = 345/434 (79.49%), Postives = 370/434 (85.25%), Query Frame = 0

Query: 1   MDWTRGPAVGRGSSATVYLATASSSGHQFAVKSTDLSSSALLQKEHALLSNFSSPYLIKC 60
           M WTRGPAVGRGSSATVYLAT +SSGH FAVKSTDLSSSALLQKEHALLS  SSPYLI C
Sbjct: 1   MVWTRGPAVGRGSSATVYLATDASSGHHFAVKSTDLSSSALLQKEHALLSKLSSPYLINC 60

Query: 61  LGFDIETEAFNPPVFNLFMEYLPEGTLWDAIQSNGGRMEESTIGVFSRQILKGLEYLHAN 120
           LGFDIETE F  PVFNLFMEYLP+GTLWDAIQ NGGRMEESTI VFSRQILKGLEYLHAN
Sbjct: 61  LGFDIETETFKLPVFNLFMEYLPQGTLWDAIQRNGGRMEESTIRVFSRQILKGLEYLHAN 120

Query: 121 GLAHCDIKSRNLLLSGEGLKIADLGCAKFVEGISGNRIGTFSGSPAFMAPEVARGEEQGF 180
           GLAHCDIKSRNLL+S  GLKIADLGCAKFV+G+SGN +  FSGSPAFMAPEVARGEEQGF
Sbjct: 121 GLAHCDIKSRNLLMSDAGLKIADLGCAKFVDGVSGNGVRDFSGSPAFMAPEVARGEEQGF 180

Query: 181 PADVWAFGCTIIEMATGDHPWPEMEDPISALYRIGFSDELPEIPRWLSKSARDFVAKCLI 240
           PAD+WAFGCT+IEMATG+HPW +++DP+SALYRIGF+ ELPE PRWLS+ ARDFVAKCL+
Sbjct: 181 PADIWAFGCTVIEMATGEHPWADIKDPVSALYRIGFTGELPEAPRWLSERARDFVAKCLL 240

Query: 241 KDPKERWTVKQLLEHPFLQGFDSESEIECWRMKTMRNSSPSCVLDQSFWDSMEDLETPLK 300
           K PKERWT KQLLEHPFLQ FDSESEIE  RMKTM NSSP CVLDQSFWDSMED +TPLK
Sbjct: 241 KVPKERWTAKQLLEHPFLQEFDSESEIESSRMKTM-NSSPRCVLDQSFWDSMEDSKTPLK 300

Query: 301 VTHRGSLADGPAERIRSLTGNLSSDWDLEWDDRDWVLIRNNGVGGGSHGNGSVSSDSSVT 360
           VTH+ SL D PAERIRSL GNLSSDWDLE DD DW+LIRNNGVGGGSHGNGSVSSD S T
Sbjct: 301 VTHQASLEDCPAERIRSLAGNLSSDWDLEGDDGDWILIRNNGVGGGSHGNGSVSSDFSDT 360

Query: 361 VEEEEEEEEEEEILNLEFEENSVLFSLVNDDFSIVNSRNAELEMEIKSDFMLETVRFGVK 420
              + EEEEE EILNLEFEENSVLF   +DD  I+N+    LEMEI         RFG K
Sbjct: 361 ---DGEEEEELEILNLEFEENSVLFCSYDDDSGIMNNGRGGLEMEIG--------RFGGK 420

Query: 421 KYCSSSSLIQIHLL 435
           K C+ SSL+Q  LL
Sbjct: 421 K-CNCSSLMQFDLL 421

BLAST of Bhi09G001396 vs. ExPASy TrEMBL
Match: A0A6J1F7Q1 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=3662 GN=LOC111442896 PE=3 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 5.7e-189
Identity = 345/435 (79.31%), Postives = 368/435 (84.60%), Query Frame = 0

Query: 1   MDWTRGPAVGRGSSATVYLATASSSGHQFAVKSTDLSSSALLQKEHALLSNFSSPYLIKC 60
           M WTRGPAVGRGSSATVYLAT +SSGH FAVKSTDLSSSALLQKEHALLS  SSPYLI C
Sbjct: 1   MVWTRGPAVGRGSSATVYLATDASSGHHFAVKSTDLSSSALLQKEHALLSKLSSPYLINC 60

Query: 61  LGFDIETEAFNPPVFNLFMEYLPEGTLWDAIQSNGGRMEESTIGVFSRQILKGLEYLHAN 120
           LGFDIETE F  PVFNLFMEY PEGTLWDAIQS+GGRMEES I VFSRQILKGLEYLHAN
Sbjct: 61  LGFDIETETFKIPVFNLFMEYFPEGTLWDAIQSSGGRMEESAIRVFSRQILKGLEYLHAN 120

Query: 121 GLAHCDIKSRNLLLSGEGLKIADLGCAKFVEGISGNRIGTFSGSPAFMAPEVARGEEQGF 180
           GLAHCDIKSRNLLLS  GLKIADLGCAKFV+G+S N +  FSGSPAFMAPEVARGEEQGF
Sbjct: 121 GLAHCDIKSRNLLLSDAGLKIADLGCAKFVDGVSENGVRDFSGSPAFMAPEVARGEEQGF 180

Query: 181 PADVWAFGCTIIEMATGDHPWPEMEDPISALYRIGFSDELPEIPRWLSKSARDFVAKCLI 240
           PAD+WAFGCT+IEMATG+HPW +++DP+SALYRIGF+ ELPE PRWLS+ ARDFVAKCLI
Sbjct: 181 PADIWAFGCTVIEMATGEHPWADIKDPVSALYRIGFTGELPEAPRWLSEWARDFVAKCLI 240

Query: 241 KDPKERWTVKQLLEHPFLQGFDSESEIECWRMKTMRNSSPSCVLDQSFWDSMEDLETPLK 300
           K PKERWT KQLLEHPFLQ FDSESEIE  RMKT+ NSSP CVLDQSFWDSMED +TPLK
Sbjct: 241 KVPKERWTAKQLLEHPFLQEFDSESEIESSRMKTV-NSSPRCVLDQSFWDSMEDSKTPLK 300

Query: 301 VTHRGSLADGPAERIRSLTGNLSSDWDLEWDDRDWVLIRNNGVGGGSHGNGSVSSDSSVT 360
           VTH+ SL D PAERIRSL GNLSSDWDLE DD DW+LIRNNGVGGGSHGNGSVSSD S T
Sbjct: 301 VTHQASLEDCPAERIRSLAGNLSSDWDLEGDDGDWILIRNNGVGGGSHGNGSVSSDFSAT 360

Query: 361 VEEEEEEEEEEEILNLEFEENSVLFSLVNDDFSIVNSRNAELEMEIKSDFMLETVRFGVK 420
           V    EEEEE EILNLEFEENSVLF   +DD  I+N+    LEMEI         RFG K
Sbjct: 361 V----EEEEELEILNLEFEENSVLFCSYDDDSGIMNNGRGGLEMEIG--------RFGGK 420

Query: 421 KYCSSSSLIQIHLLF 436
             C+ SSL+Q  LLF
Sbjct: 421 N-CNCSSLMQFDLLF 421

BLAST of Bhi09G001396 vs. ExPASy TrEMBL
Match: A0A6J1FKH1 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=3662 GN=LOC111446257 PE=3 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 6.1e-183
Identity = 336/440 (76.36%), Postives = 371/440 (84.32%), Query Frame = 0

Query: 1   MDWTRGPAVGRGSSATVYLA---TASSSGHQFAVKSTDLSSSALLQKEHALLSNFSSPYL 60
           MDWTRGPAVGRGSSATVYLA   T+SS+  QFAVK+TDLS+S LL+KEHALLSN +SPYL
Sbjct: 1   MDWTRGPAVGRGSSATVYLATTTTSSSTSRQFAVKTTDLSTSELLRKEHALLSNLNSPYL 60

Query: 61  IKCLGFDIETEAFNPPVFNLFMEYLPEGTLWDAIQSNGGRMEESTIGVFSRQILKGLEYL 120
           I C GFD+E E+   PVFNLFMEYLPEGTLWDAIQ +GGRMEES IG +SRQILKGLEYL
Sbjct: 61  INCFGFDVEVESSKSPVFNLFMEYLPEGTLWDAIQRHGGRMEESMIGAYSRQILKGLEYL 120

Query: 121 HANGLAHCDIKSRNLLLSGEGLKIADLGCAKFVE--GISGNRIGTFSGSPAFMAPEVARG 180
           HANGLAHCDIKSRNLLLSGEGLKIADLGCAKFVE  G+SGN +  FSGSPA+MAPEVARG
Sbjct: 121 HANGLAHCDIKSRNLLLSGEGLKIADLGCAKFVEGGGVSGNGVRAFSGSPAYMAPEVARG 180

Query: 181 EEQGFPADVWAFGCTIIEMATGDHPWPEMEDPISALYRIGFSDELPEIPRWLSKSARDFV 240
           EEQGFPADVWAFGCT+IEMATG+HPW  +EDP+SALY IGFS E+PEIPRWLS+ ARDFV
Sbjct: 181 EEQGFPADVWAFGCTVIEMATGNHPWTGLEDPVSALYTIGFSGEVPEIPRWLSEEARDFV 240

Query: 241 AKCLIKDPKERWTVKQLLEHPFLQGFDSESEIECWRMKTMRNSSPSCVLDQSFWDSMEDL 300
           AKCLIKDPKERW+VK+LLEHPFLQGFDSESEIEC R KT  +SSPS VLDQSFWDSMEDL
Sbjct: 241 AKCLIKDPKERWSVKRLLEHPFLQGFDSESEIECSRKKT-TSSSPSSVLDQSFWDSMEDL 300

Query: 301 ETPLKVTHRGSLADGPAERIRSLTGNLSSDWDLEWDD-RDWVLIRNNGVGGGSHGNGSVS 360
           ETPLKV+H+ SLAD PAERIRSL GNLSS+ D EWDD RDWVLIRNN V GGSHGNGSVS
Sbjct: 301 ETPLKVSHQASLADSPAERIRSLAGNLSSNCDWEWDDERDWVLIRNNDVDGGSHGNGSVS 360

Query: 361 SDSSVTVEEEEEEEEEEEILNLEFEENSVLFSLVNDDFSIVNSRNAELEMEIKSDFMLET 420
           SD      EE+EEEEE   LNLEFEE+ V F L++DDFSI+N       +E ++DFML T
Sbjct: 361 SD----FPEEKEEEEEMVTLNLEFEEDLVSFCLIDDDFSIMN-------IEFETDFMLNT 420

Query: 421 VRFGVKKYCSSSSLIQIHLL 435
           V+FG      S SL+QI  L
Sbjct: 421 VQFGGSNEYCSYSLMQIDRL 428

BLAST of Bhi09G001396 vs. ExPASy TrEMBL
Match: A0A6J1IUQ7 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=3661 GN=LOC111480765 PE=3 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 5.7e-181
Identity = 334/440 (75.91%), Postives = 368/440 (83.64%), Query Frame = 0

Query: 1   MDWTRGPAVGRGSSATVYLA---TASSSGHQFAVKSTDLSSSALLQKEHALLSNFSSPYL 60
           MDWTRGPAVGRGSSATVYLA   T+SSS  QFAVK+TDLS+S LL+KEHALLSN +SPYL
Sbjct: 1   MDWTRGPAVGRGSSATVYLATTTTSSSSSRQFAVKTTDLSTSELLRKEHALLSNLNSPYL 60

Query: 61  IKCLGFDIETEAFNPPVFNLFMEYLPEGTLWDAIQSNGGRMEESTIGVFSRQILKGLEYL 120
           I C GFD+E E+   PVFNLFMEYLPEGTLWDAIQ +GGRMEES IG +SRQILKGLEYL
Sbjct: 61  INCFGFDVEIESSKSPVFNLFMEYLPEGTLWDAIQRHGGRMEESMIGAYSRQILKGLEYL 120

Query: 121 HANGLAHCDIKSRNLLLSGEGLKIADLGCAKFVE--GISGNRIGTFSGSPAFMAPEVARG 180
           HANGLAHCDIKSRNLLLSGEGLKIADLGCAKFVE  G+SGN +  FSGSPA+MAPEVARG
Sbjct: 121 HANGLAHCDIKSRNLLLSGEGLKIADLGCAKFVEGGGVSGNGVRAFSGSPAYMAPEVARG 180

Query: 181 EEQGFPADVWAFGCTIIEMATGDHPWPEMEDPISALYRIGFSDELPEIPRWLSKSARDFV 240
           EEQGFPADVWAFGCT+IEMATG+HPW  +EDP+SALY IGFS E+PE+PRWLS+ ARDF+
Sbjct: 181 EEQGFPADVWAFGCTVIEMATGNHPWTGLEDPVSALYTIGFSGEVPEVPRWLSEEARDFI 240

Query: 241 AKCLIKDPKERWTVKQLLEHPFLQGFDSESEIECWRMKTMRNSSPSCVLDQSFWDSMEDL 300
           AKC IKDPKERW+VK+LLEHPFLQGFDSESEIEC R KT  +SSPS VLDQSFWDSMED 
Sbjct: 241 AKCSIKDPKERWSVKRLLEHPFLQGFDSESEIECSRRKT-TSSSPSSVLDQSFWDSMEDS 300

Query: 301 ETPLKVTHRGSLADGPAERIRSLTGNLSSDWDLEWDD-RDWVLIRNNGVGGGSHGNGSVS 360
           ETPLKV+++  LAD PAERIRSL GNLSS+ D E DD RDW+LIRNN VGGGSHGNGSVS
Sbjct: 301 ETPLKVSNQAPLADSPAERIRSLAGNLSSNCDWEGDDERDWILIRNNDVGGGSHGNGSVS 360

Query: 361 SDSSVTVEEEEEEEEEEEILNLEFEENSVLFSLVNDDFSIVNSRNAELEMEIKSDFMLET 420
           SD     EEEEEEEEE   LNLEFEE+ VLF LV+DDFS  N       ME ++DFML T
Sbjct: 361 SDFP---EEEEEEEEEMVTLNLEFEEDLVLFCLVDDDFSTTN-------MEFETDFMLNT 420

Query: 421 VRFGVKKYCSSSSLIQIHLL 435
           V+FG      S SL+QI  L
Sbjct: 421 VKFGGSNEYCSYSLMQIDRL 429

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G26890.12.1e-9547.88mitogen-activated protein kinase kinase kinase 16 [more]
AT5G55090.19.9e-9347.37mitogen-activated protein kinase kinase kinase 15 [more]
AT2G32510.16.2e-7943.91mitogen-activated protein kinase kinase kinase 17 [more]
AT1G05100.18.4e-7645.33mitogen-activated protein kinase kinase kinase 18 [more]
AT4G36950.17.4e-5637.61mitogen-activated protein kinase kinase kinase 21 [more]
Match NameE-valueIdentityDescription
O808888.7e-7843.91Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
Q9ZVP51.2e-7445.33Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370... [more]
Q9SND63.3e-5336.44Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
Q54R823.7e-4434.06Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=... [more]
O220422.4e-4336.53Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A1S3BIX81.9e-20081.15mitogen-activated protein kinase kinase kinase 2 OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1IHI81.1e-18979.49mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=36... [more]
A0A6J1F7Q15.7e-18979.31mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=... [more]
A0A6J1FKH16.1e-18376.36mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=... [more]
A0A6J1IUQ75.7e-18175.91mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=36... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 359..381
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 2..303
e-value: 4.0E-69
score: 235.3
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 74..269
e-value: 5.2E-15
score: 53.0
NoneNo IPR availablePIRSRPIRSR630220-2PIRSR630220-2coord: 76..250
e-value: 3.6E-11
score: 39.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 346..369
NoneNo IPR availablePANTHERPTHR48011:SF76MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 15coord: 1..356
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 1..356
NoneNo IPR availableCDDcd06606STKc_MAPKKKcoord: 2..258
e-value: 3.10694E-125
score: 361.453
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 3..258
e-value: 1.4E-72
score: 257.1
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 7..258
e-value: 5.4E-53
score: 180.0
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 3..258
score: 39.961777
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 9..32
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 122..134
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 3..298

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi09M001396Bhi09M001396mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity