Bhi09G001117 (gene) Wax gourd (B227) v1

Overview


Name	Bhi09G001117
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	pectinesterase inhibitor-like
Location	chr9: 33627069 .. 33627617 (-)
RNA-Seq Expression	Bhi09G001117
Synteny	Bhi09G001117

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAATTCCATTATTCTTCCAGCCTCTTCTCTTCTACCTTTCGTCCTTTCACTTCTCTTCTTTCATGTTGTCGTTCCCATGCATGCAGCTTCCCCAAACGACGTCGTTTCCACCATCTGCCAAAGAACTAGAAACCCTCCCTTTTGCTCCAACCTCTTGAAATCTGCTCGCACCACAGACCTGAAAGGGCTAATCACTTTCACCATCAACCTCGCCCACACCAAGGCTACGGAAAGTCGAGCCCTCGCCCAGTTCCTGGCATCGAAGGCAGCCGACCCGAAGTTCAAGGAGCGCTACGCCTCCTGTGCCAAACACTACGACGACGCTGCCAGTGACCTCAACGATGCGAAGAACTATTTGGCCACAGGTGATTACGATGGCGTTAATATTCAGGCTTCTGGAGCCATGACCGAGACCGACGATTGTCAGGATAACTTCACGCAATCACCGATGGACGGATCGGGGTTGTCCAAACATGGCAAGGCTCTGGAAGATATTTGTAGTATCATCTTGGTTATAGCCAATATTCTCCTTGGACGTGTCTAA

mRNA sequence

ATGGCAAATTCCATTATTCTTCCAGCCTCTTCTCTTCTACCTTTCGTCCTTTCACTTCTCTTCTTTCATGTTGTCGTTCCCATGCATGCAGCTTCCCCAAACGACGTCGTTTCCACCATCTGCCAAAGAACTAGAAACCCTCCCTTTTGCTCCAACCTCTTGAAATCTGCTCGCACCACAGACCTGAAAGGGCTAATCACTTTCACCATCAACCTCGCCCACACCAAGGCTACGGAAAGTCGAGCCCTCGCCCAGTTCCTGGCATCGAAGGCAGCCGACCCGAAGTTCAAGGAGCGCTACGCCTCCTGTGCCAAACACTACGACGACGCTGCCAGTGACCTCAACGATGCGAAGAACTATTTGGCCACAGGTGATTACGATGGCGTTAATATTCAGGCTTCTGGAGCCATGACCGAGACCGACGATTGTCAGGATAACTTCACGCAATCACCGATGGACGGATCGGGGTTGTCCAAACATGGCAAGGCTCTGGAAGATATTTGTAGTATCATCTTGGTTATAGCCAATATTCTCCTTGGACGTGTCTAA

Coding sequence (CDS)

ATGGCAAATTCCATTATTCTTCCAGCCTCTTCTCTTCTACCTTTCGTCCTTTCACTTCTCTTCTTTCATGTTGTCGTTCCCATGCATGCAGCTTCCCCAAACGACGTCGTTTCCACCATCTGCCAAAGAACTAGAAACCCTCCCTTTTGCTCCAACCTCTTGAAATCTGCTCGCACCACAGACCTGAAAGGGCTAATCACTTTCACCATCAACCTCGCCCACACCAAGGCTACGGAAAGTCGAGCCCTCGCCCAGTTCCTGGCATCGAAGGCAGCCGACCCGAAGTTCAAGGAGCGCTACGCCTCCTGTGCCAAACACTACGACGACGCTGCCAGTGACCTCAACGATGCGAAGAACTATTTGGCCACAGGTGATTACGATGGCGTTAATATTCAGGCTTCTGGAGCCATGACCGAGACCGACGATTGTCAGGATAACTTCACGCAATCACCGATGGACGGATCGGGGTTGTCCAAACATGGCAAGGCTCTGGAAGATATTTGTAGTATCATCTTGGTTATAGCCAATATTCTCCTTGGACGTGTCTAA

Protein sequence

MANSIILPASSLLPFVLSLLFFHVVVPMHAASPNDVVSTICQRTRNPPFCSNLLKSARTTDLKGLITFTINLAHTKATESRALAQFLASKAADPKFKERYASCAKHYDDAASDLNDAKNYLATGDYDGVNIQASGAMTETDDCQDNFTQSPMDGSGLSKHGKALEDICSIILVIANILLGRV

Homology

[Close]

BLAST of Bhi09G001117 vs. TAIR 10
Match: AT3G17220.1 (pectin methylesterase inhibitor 2 )

HSP 1 Score: 94.0 bits (232), Expect = 1.4e-19
Identity = 59/164 (35.98%), Postives = 88/164 (53.66%), Query Frame = 0


Query: 18  SLLFFHVVVPMHAASPNDVVSTICQRTRNPPFCSNLLKSARTT---DLKGLITFTINLAH 77
           SL+FF +   ++A   +  +  IC + +N  FC++ +KS   T   DL+ L   T   A 
Sbjct: 13  SLMFFVMTGSLNAQVAD--IKAICGKAKNQSFCTSYMKSNPKTSGADLQTLANITFGSAQ 72

Query: 78  TKATESRALAQFLASKAADPKFKERYASCAKHYDDAASDLNDAKNYLATGDYDGVNIQAS 137
           T A+E     Q L   A +P  K+ Y SC +HY  A S LNDAK  LA+GD  G+NI+ S
Sbjct: 73  TSASEGFRKIQSLVKTATNPTMKKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLNIKVS 132

Query: 138 GAMTETDDCQDNFTQSPMDGSGLSKHGKALEDICSIILVIANIL 179
            AM     C+ +     +D S +   G   ++IC I+LVI+N++
Sbjct: 133 AAMEGPSTCEQDMADFKVDPSAVKNSGD-FQNICGIVLVISNMM 173

[Close]

BLAST of Bhi09G001117 vs. TAIR 10
Match: AT1G48020.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 84.0 bits (206), Expect = 1.4e-16
Identity = 51/165 (30.91%), Postives = 91/165 (55.15%), Query Frame = 0


Query: 15  FVLSLLFFHVVVPMHAASPNDVVSTICQRTRNPPFCSNLLKSA-RTTDLKGLITFTINLA 74
           F+ SL+F  ++   +A + ++ +STIC +T NP FC   L +   + +L+ L   T++  
Sbjct: 10  FLSSLMFLLLIGSSYAITSSE-MSTICDKTLNPSFCLKFLNTKFASPNLQALAKTTLDST 69

Query: 75  HTKATESRALAQFLASKAADPKFKERYASCAKHYDDAASDLNDAKNYLATGDYDGVNIQA 134
             +AT++    Q +     DP+ K  Y SC   Y+ A  +L +A  +LA+GD  G+N++ 
Sbjct: 70  QARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMKV 129

Query: 135 SGAMTETDDCQDNFTQSPMDGSGLSKHGKALEDICSIILVIANIL 179
           S A+   D C D+  +     S +  + K ++++C I LVI+N+L
Sbjct: 130 SAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNML 173

[Close]

BLAST of Bhi09G001117 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 109.4 bits (272), Expect = 4.4e-23
Identity = 65/173 (37.57%), Postives = 98/173 (56.65%), Query Frame = 0


Query: 15  FVLSLLFFHVVVPM----HAASPNDVVSTICQRTRNPPFCSNLLKS---ARTTDLKGLIT 74
           FV  LL    + P+       + N ++S IC +TRNP  C   L+S   + + DLKGL  
Sbjct: 11  FVSLLLVILFISPLSQRPSVKAENHLISEICPKTRNPSLCLQALESDPRSASKDLKGLGQ 70

Query: 75  FTINLAHTKATESRALAQFLASKAADPKFKERYASCAKHYDDAASDLNDAKNYLATGDYD 134
           F+I++A   A ++  +   L ++A DPK K RY +C+++Y DA   L  AK +L +GDY+
Sbjct: 71  FSIDIAQASAKQTSKIIASLTNQATDPKLKGRYETCSENYADAIDSLGQAKQFLTSGDYN 130

Query: 135 GVNIQASGAMTETDDCQDNFTQSPMDGSGLSKHGKALEDICSIILVIANILLG 181
            +NI AS A      C+D+F   P   + L +    LED+C I+LVI+N+L G
Sbjct: 131 SLNIYASAAFDGAGTCEDSFEGPPNIPTQLHQADLKLEDLCDIVLVISNLLPG 183

[Close]

BLAST of Bhi09G001117 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.9e-18
Identity = 59/164 (35.98%), Postives = 88/164 (53.66%), Query Frame = 0


Query: 18  SLLFFHVVVPMHAASPNDVVSTICQRTRNPPFCSNLLKSARTT---DLKGLITFTINLAH 77
           SL+FF +   ++A   +  +  IC + +N  FC++ +KS   T   DL+ L   T   A 
Sbjct: 13  SLMFFVMTGSLNAQVAD--IKAICGKAKNQSFCTSYMKSNPKTSGADLQTLANITFGSAQ 72

Query: 78  TKATESRALAQFLASKAADPKFKERYASCAKHYDDAASDLNDAKNYLATGDYDGVNIQAS 137
           T A+E     Q L   A +P  K+ Y SC +HY  A S LNDAK  LA+GD  G+NI+ S
Sbjct: 73  TSASEGFRKIQSLVKTATNPTMKKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLNIKVS 132

Query: 138 GAMTETDDCQDNFTQSPMDGSGLSKHGKALEDICSIILVIANIL 179
            AM     C+ +     +D S +   G   ++IC I+LVI+N++
Sbjct: 133 AAMEGPSTCEQDMADFKVDPSAVKNSGD-FQNICGIVLVISNMM 173

[Close]

BLAST of Bhi09G001117 vs. ExPASy Swiss-Prot
Match: Q9LNF2 (Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.0e-15
Identity = 51/165 (30.91%), Postives = 91/165 (55.15%), Query Frame = 0


Query: 15  FVLSLLFFHVVVPMHAASPNDVVSTICQRTRNPPFCSNLLKSA-RTTDLKGLITFTINLA 74
           F+ SL+F  ++   +A + ++ +STIC +T NP FC   L +   + +L+ L   T++  
Sbjct: 10  FLSSLMFLLLIGSSYAITSSE-MSTICDKTLNPSFCLKFLNTKFASPNLQALAKTTLDST 69

Query: 75  HTKATESRALAQFLASKAADPKFKERYASCAKHYDDAASDLNDAKNYLATGDYDGVNIQA 134
             +AT++    Q +     DP+ K  Y SC   Y+ A  +L +A  +LA+GD  G+N++ 
Sbjct: 70  QARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMKV 129

Query: 135 SGAMTETDDCQDNFTQSPMDGSGLSKHGKALEDICSIILVIANIL 179
           S A+   D C D+  +     S +  + K ++++C I LVI+N+L
Sbjct: 130 SAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNML 173

[Close]

BLAST of Bhi09G001117 vs. ExPASy TrEMBL
Match: A0A6J1GBD1 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111452619 PE=4 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 5.9e-55
Identity = 113/156 (72.44%), Postives = 130/156 (83.33%), Query Frame = 0


Query: 25  VVPMHAASPNDVVSTICQRTRNPPFCSNLLKSARTTDLKGLITFTINLAHTKATESRALA 84
           +VPMHAAS ND+VSTIC++TRNP FC N+LKSA TTDLKGL TFT+NLAH KAT+S ALA
Sbjct: 1   MVPMHAASQNDIVSTICKKTRNPSFCFNVLKSAGTTDLKGLATFTLNLAHDKATQSYALA 60

Query: 85  QFLASKAADPKFKERYASCAKHYDDAASDLNDAKNYLATGDYDGVNIQASGAMTETDDCQ 144
           Q LASKA DPK KERYA+CA+ YDDA  D+ D KNYL  GDY+GVNI+AS AMTE +DC 
Sbjct: 61  QSLASKAVDPKLKERYATCAELYDDAVDDIEDGKNYLGEGDYNGVNIKASAAMTEANDCL 120

Query: 145 DNFTQSPMDGSGLSKHGKALEDICSIILVIANILLG 181
           D+FTQ P D S LS +GK +EDICSIILVIAN+LLG
Sbjct: 121 DSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLG 156

[Close]

BLAST of Bhi09G001117 vs. ExPASy TrEMBL
Match: A0A6J1JRP2 (pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111488294 PE=4 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 1.0e-54
Identity = 118/181 (65.19%), Postives = 140/181 (77.35%), Query Frame = 0


Query: 1   MANSIILPASSLLPFVLSLLFFHVVVPMHAASPNDVVSTICQRTRNPPFCSNLLKSARTT 60
           M +S  L  SSLL FVLSL FF+ VVPMHA+ PN V STIC++T+NP FC N+LKSA TT
Sbjct: 1   MVSSTFLAVSSLLAFVLSLFFFNGVVPMHASFPNSVPSTICKKTKNPSFCFNILKSAGTT 60

Query: 61  DLKGLITFTINLAHTKATESRALAQFLASKAADPKFKERYASCAKHYDDAASDLNDAKNY 120
           DLKGL TF +NLAH KAT++RALA  LASKA D K KERYA+C KHYD+AA+D+ DAK  
Sbjct: 61  DLKGLATFALNLAHNKATKTRALAPSLASKATDRKLKERYATCVKHYDNAAADIKDAKYN 120

Query: 121 LATGDYDGVNIQASGAMTETDDCQDNFTQSPMDGSGLSKHGKALEDICSIILVIANILLG 180
           L  GDY+G NIQASGAMTE  DC DNFT  P + S LS + K +ED+CSIIL+I+N+LL 
Sbjct: 121 LGKGDYNGANIQASGAMTEAGDCLDNFTLLPKNPSVLSNNEKVVEDMCSIILIISNLLLV 180

Query: 181 R 182
           R
Sbjct: 181 R 181

[Close]

BLAST of Bhi09G001117 vs. ExPASy TrEMBL
Match: A0A6J1EQC5 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111434861 PE=4 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 2.2e-54
Identity = 120/181 (66.30%), Postives = 140/181 (77.35%), Query Frame = 0


Query: 1   MANSIILPASSLLPFVLSLLFFHVVVPMHAASPNDVVSTICQRTRNPPFCSNLLKSARTT 60
           MA+S  L  S +   VLS+LFF+ V+PMHAAS NDV STIC++TRNP FC ++LKSA TT
Sbjct: 1   MASSSFLAVSLV---VLSVLFFNGVMPMHAASQNDVASTICKKTRNPSFCFDVLKSAGTT 60

Query: 61  DLKGLITFTINLAHTKATESRALAQFLASKAADPKFKERYASCAKHYDDAASDLNDAKNY 120
           DLKGL TFT+NLAH K  ESRALAQ LASKA DPK KERYA+CA+ YD+A SD+ D K  
Sbjct: 61  DLKGLATFTLNLAHDKTVESRALAQSLASKAIDPKLKERYATCAEQYDNAISDIEDGKVN 120

Query: 121 LATGDYDGVNIQASGAMTETDDCQDNFTQSPMDGSGLSKHGKALEDICSIILVIANILLG 180
           L  GDY+GVN +AS AMTE  DC D+F Q P D S LS +GK +EDICSIILVIAN+LLG
Sbjct: 121 LGEGDYNGVNTKASAAMTEAGDCLDSFAQPPKDSSALSGNGKTVEDICSIILVIANLLLG 178

Query: 181 R 182
           R
Sbjct: 181 R 178

[Close]

BLAST of Bhi09G001117 vs. ExPASy TrEMBL
Match: A0A6J1IEN0 (pectinesterase inhibitor-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111471996 PE=4 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 2.9e-54
Identity = 118/181 (65.19%), Postives = 143/181 (79.01%), Query Frame = 0


Query: 1   MANSIILPASSLLPFVLSLLFFHVVVPMHAASPNDVVSTICQRTRNPPFCSNLLKSARTT 60
           MA+S  L   ++   VL +LFF+ +VPMHAAS +D++STIC++TRNP FC N+LKSA TT
Sbjct: 1   MASSSFL---AVFRIVLLVLFFNGMVPMHAASQDDILSTICKKTRNPSFCFNVLKSAGTT 60

Query: 61  DLKGLITFTINLAHTKATESRALAQFLASKAADPKFKERYASCAKHYDDAASDLNDAKNY 120
           DLK L TFT+NLAH K  ++RALAQ LASKA+DPK KERYA+CA+ Y +AA D+ D K  
Sbjct: 61  DLKRLATFTLNLAHDKVAQTRALAQSLASKASDPKLKERYATCAEQYGEAADDIKDGKKD 120

Query: 121 LATGDYDGVNIQASGAMTETDDCQDNFTQSPMDGSGLSKHGKALEDICSIILVIANILLG 180
           L  GDY+GVNI+AS AMTE  DCQDNFTQSP D S LS +GK +EDICSIILVIAN+LLG
Sbjct: 121 LGEGDYNGVNIKASAAMTEAGDCQDNFTQSPKDPSALSGNGKTVEDICSIILVIANLLLG 178

Query: 181 R 182
           R
Sbjct: 181 R 178

[Close]

BLAST of Bhi09G001117 vs. ExPASy TrEMBL
Match: A0A6J1F4D5 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440166 PE=4 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 5.0e-54
Identity = 118/181 (65.19%), Postives = 142/181 (78.45%), Query Frame = 0


Query: 1   MANSIILPASSLLPFVLSLLFFHVVVPMHAASPNDVVSTICQRTRNPPFCSNLLKSARTT 60
           MA+S  L   ++   VL +LFF+ +VPMHAAS +D+VSTIC++TRNP FC N+LKSA TT
Sbjct: 1   MASSSFL---AVFRIVLLVLFFNGMVPMHAASQDDIVSTICKKTRNPSFCFNVLKSAGTT 60

Query: 61  DLKGLITFTINLAHTKATESRALAQFLASKAADPKFKERYASCAKHYDDAASDLNDAKNY 120
           DLKGL TFT+NLAH K  ++ ALA  LASKAADPK K+RYA+CA+ YDDAA D+ D KN 
Sbjct: 61  DLKGLATFTLNLAHDKVAQTHALALSLASKAADPKLKDRYATCAEQYDDAADDIEDGKND 120

Query: 121 LATGDYDGVNIQASGAMTETDDCQDNFTQSPMDGSGLSKHGKALEDICSIILVIANILLG 180
           L  GDY+GVNI+AS AMTE  DC D+FTQ P D S LS +GK +EDICSIILVIAN+LLG
Sbjct: 121 LREGDYNGVNIKASAAMTEAGDCLDSFTQPPKDPSALSGNGKTVEDICSIILVIANLLLG 178

Query: 181 R 182
           R
Sbjct: 181 R 178

The following BLAST results are available for this feature:

BLAST of Bhi09G001117 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G17220.1	1.4e-19	35.98	pectin methylesterase inhibitor 2	[more]
AT1G48020.1	1.4e-16	30.91	pectin methylesterase inhibitor 1	[more]

BLAST of Bhi09G001117 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P83326	4.4e-23	37.57	Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2	[more]
Q9LUV1	1.9e-18	35.98	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1	[more]
Q9LNF2	2.0e-15	30.91	Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1	[more]

BLAST of Bhi09G001117 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GBD1	5.9e-55	72.44	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111452619 PE=4...	[more]
A0A6J1JRP2	1.0e-54	65.19	pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111488294 PE=4 S...	[more]
A0A6J1EQC5	2.2e-54	66.30	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111434861 PE=4...	[more]
A0A6J1IEN0	2.9e-54	65.19	pectinesterase inhibitor-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A6J1F4D5	5.0e-54	65.19	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111440166 PE=4...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 32..174 e-value: 1.1E-27 score: 107.9
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 36..174 e-value: 1.1E-21 score: 77.7
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 12..178 e-value: 1.5E-34 score: 116.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 32..179 e-value: 3.5E-35 score: 123.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 34..179
None	No IPR available	PANTHER	PTHR36710	PECTINESTERASE INHIBITOR-LIKE	coord: 11..178
None	No IPR available	PANTHER	PTHR36710:SF6	PECTINESTERASE INHIBITOR-LIKE	coord: 11..178
IPR034086	Pectinesterase inhibitor, plant	CDD	cd15797	PMEI	coord: 33..178 e-value: 1.03597E-43 score: 140.635

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi09M001117	Bhi09M001117	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity