Bhi09G000804 (gene) Wax gourd (B227) v1

Overview
NameBhi09G000804
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionAuxin response factor
Locationchr9: 22107974 .. 22111667 (+)
RNA-Seq ExpressionBhi09G000804
SyntenyBhi09G000804
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCTACTAAACCAAACCATAAAAAAGCCTACAGCGTGAGCTCAGCGATCACTATAATTCATACAATTTCACCGATAAATCATATCCACACGTCTCAATCGCCCTGCCATCAACCGTTTGTGAGCTTGGAAAATCACAAAGAATCAGCAAGAAATGGAGTCTCTGATTTTGATTCCAGCAAGTAAACACGTTCCAATCACAAATTCTTCTCTATTTTGCTCCGGTTTCTCCATTCCATTCACTTCTAGAAAGCGCGCCATCAATTTCCAACCATGTTGTCCACAGATTCTTCATTGCAATGGCCTTCGACATGCGCAAGCAACTCCGAGGTCTTATATTGGAAATGGACCGCCGTATGGTGAGGAAGATGATGATCCTGAACTACAGGTTGAAAGCCTCAGGGTTCCTGATGATTGGTCGGTTCCTTCCAAGGCATTGGAGGTATTGTTTTCCGTTCTTCGTTTTTGTTGTTGATTTTCGATCTGCATTTCTGTACTTGTTCGATTAGGTTACTTGTCCAACTTCTTTTTAGTGATTTGAACGTAGTAATTGGTTTTGTTCTTGATGATGGCGCATTCTTGATGTTTTTGAATGATGAATGCGATGTGATGACATTGTTATCTTCATGAAATTTAAGCAGAAATTAGACATTCTCCTTCGAACGTCGTGTTGCTAACTAAATTCAGTGTGAAGACCTCAGAGTTGGGTTTTTTAAACTGGCCCGAATTTGATGTTTACTCTGTATTAGCCTACGGAAAGAATATTCTAGAGCCACCACACAATGTTCTTGTCGACACAAATTTGTTCTGCCACTAGAGTATGTTGGGCATGACCTTTGATGCCTTTTCCCAATGAATAATTTATTATAGAAAAAGAAAATTGGGTCTGGATGTAAAATGGATTTTGATTTATGAGCTGGGAAAGATTTTCAAGTTAGTACCAATAAAATTAGGCTCCAAAGTCAAATCTAGGTTGCCACTATTTTACATTTGTTTGAATGATCCAAGACAGTTTTGATCATTCTGCTTGAGTAGTTTACGAGAAGGAGATTGGAAATCCTTGAAATTAGTCTTGGTATATCCTATTAGATCTGCTGGTTTCTTCTATATAATGGCTTAGAATTAAATCTATTTGTCCAATTATCTGGTGGAAGTTCTTCTCGATGCTTATTTCTCCAAGCTTGCAGAAAAGTTATAGCTTGGATAAGTTTGAGTGATCAGATTTACTGTCTCAGTGTAGCTCTTTTCACTAATCTGGCAAATGGCTGTCCTTGTCCAATTGATGGTTAAAATTTGATTTTTATTATACTTTTTTTTTAAATAGCTTTAGTTTCATCATGTTTACTCTTTCGGTGGTTGAAACCTTTAAGGGTTTGGGCTTTCGTCCGTATAGTTACATGGAACGAAGTTCCGGTGACATGTTTGGTCCTCTTTGTCAGATTATTGTGTTTGTGTTGCATCTTAGTGCTTTGGAGTCGTCTTTTTCTGTCACTGGACCAGTGAGCATTCTCCATCGGGAAAGCCTTCAAAAGGAGTTTTTTTCTTCTTCTTTTTTTTTTTTTTGCTGTTTCGAACCTCGTTTCAGTCTTCGATCATTGGGCTACAGACTTTTTATGAGTTCTTAATTGGTTGTATTTGGCTGTAATGACTATTTGTTTTTGTTTCATTTGTGTTTTTATTGTATTTTGAGCATTAGTCTGTTTCCATTTTATCAGTAAAAAATCTGACTAATCAGTTGAGATATTTTTCAGGAATCAGAATGGCTTAGGGCTACCTTGCACAAATGGTTAGATGACGAGTACTGTCCAGAGGAAACTAATGTAGAGATAAGCAAGGTTGCAGCAAATTCATATTACAATTCTTTGTTAAAGAAGAGGACAGACCTAGGCGATATTTTGTTGAATATGGCAAGAGAATTGGAATCTATTTCCTATAAGGAAAGTTTTCATGGTGCATTCTCTTCTGCCAATGCAGCAGTGAACTTAATTGCTCAAAGAATAGAGCTGTCTTAACTGTTTCCTCAACCACTTCAATTTCCAAAGTAACTAACTAATTTGTCTCCTTGACTTTTCTTTCTTACGAGTAACTCATCTGTTGTCAGATCTATATTTCTGGACAAACAATTCATGTATTGCTCTAATTCTTCACTGTCAAAGCAACTTTCAGCGACTAAGTATGGATGTTCCAAACACCAGATCTTGCACCATGCCTTTTGCCAGGTTAGCTCTAGGTAAAAGTAGAGTTTCTTCTATTGCATTTTATAGTTGTTTGACCTCAAATTATGTCTAAGAAAAGACGGCACTTCCAGAAGGGCTTTCAAGTTTGTGAGTCAAAGACAAAGCTATAATTTTAGAGGGAAGTGCTATAAAGCATAAGCTAGGGTGTCTATTTGGTCCTTTAATGATACTTGTGTAGGTTTTTCAAGTCCAGACAAAATGTACTACATATGGGAAGTTGGAGGAAGACTTGGATCATATTCTCTGAAGTCGTGAGTTTGCGAATTCCATTTGGGATTACTTCTTTTAGACATTTGGTTTCTTGCTAGCTCAACACAGGGACACCAGCATCATGATTGGATCATTCCTCCTCCATCCTCTCTTTTGTGGGAAGCGTCACTTTTTGTGGCCTGCCCAAATATGTGCTGTTTTACTGGTTCTTTGAGAGAGCATAATAACAAAGTGTTTAGAGGGTTGGAGAGGGATCCTAGTGATGTTTAGTCCTTGTCATATTCTCTGTCTCCTTTTGGGCATCAATTTTGAAGACCTTTTGTAACTATTTTATAGGAACTGTCTCGTATAGTTGGAGTCCCTTTCTTCAGCAAGCTTTCCCTTGTTTCATTAAAAAAAGCCAATCATTGGGGCTTTTGTAATTACAGCAGCTGACTTAGGTTGGAGTATTTTTTCCTGACCCTCCTAGGCAGAAGAGCTCTAATTGCTTTGGCCTTTCTTTCTCCAATGAAAGTTTGTTTCTTACATATATGTATATAGCTTCTGTTCTGAAATAATACAAATGAAGACAGAATATGAAGAACAAACGCTAGAGCAAGCATGCCAATTATACATGCTACTCATGCAATTCTGAAGTAAATCACTGGACGAACAAGGAGGACGATCAAATCTACATTCTACTCATGCAAGTGTGGAGCGAATCCCTGGATGAAAAAAGGGAGGAGGACGATACGAGCTTAACGAATCAATACGCAGGTATCAGCATTTTTACTTATTGTACAACACTACAACTAAGTATCAAAATCTAAGCTTGATTCAGACATCCTTGGAATTCTCCAGTGGCCTTAACAGTCTCATTTAGGAGGAAATTGTGATTATGTTCATGCTCTATAGTTGTTCTAGTGAGTTTATAAACATAATGAGGCTCCTTAATTTTTCTAATATGGGATTCTTAACATTATGCAATAACTTTCCTCGTAATCAATTTTTTTTAATTCAG

mRNA sequence

GCCTACTAAACCAAACCATAAAAAAGCCTACAGCGTGAGCTCAGCGATCACTATAATTCATACAATTTCACCGATAAATCATATCCACACGTCTCAATCGCCCTGCCATCAACCGTTTGTGAGCTTGGAAAATCACAAAGAATCAGCAAGAAATGGAGTCTCTGATTTTGATTCCAGCAAGTAAACACGTTCCAATCACAAATTCTTCTCTATTTTGCTCCGGTTTCTCCATTCCATTCACTTCTAGAAAGCGCGCCATCAATTTCCAACCATGTTGTCCACAGATTCTTCATTGCAATGGCCTTCGACATGCGCAAGCAACTCCGAGGTCTTATATTGGAAATGGACCGCCGTATGGTGAGGAAGATGATGATCCTGAACTACAGGTTGAAAGCCTCAGGGTTCCTGATGATTGGTCGGTTCCTTCCAAGGCATTGGAGGAATCAGAATGGCTTAGGGCTACCTTGCACAAATGGTTAGATGACGAGTACTGTCCAGAGGAAACTAATGTAGAGATAAGCAAGGTTGCAGCAAATTCATATTACAATTCTTTGTTAAAGAAGAGGACAGACCTAGGCGATATTTTGTTGAATATGGCAAGAGAATTGGAATCTATTTCCTATAAGGAAAGTTTTCATGGTGCATTCTCTTCTGCCAATGCAGCAGTGAACTTAATTGCTCAAAGAATAGAGCTGTCTTAACTGTTTCCTCAACCACTTCAATTTCCAAACGACTAAGTATGGATGTTCCAAACACCAGATCTTGCACCATGCCTTTTGCCAGACAGAATATGAAGAACAAACGCTAGAGCAAGCATGCCAATTATACATGCTACTCATGCAATTCTGAAGTAAATCACTGGACGAACAAGGAGGACGATCAAATCTACATTCTACTCATGCAAGTGTGGAGCGAATCCCTGGATGAAAAAAGGGAGGAGGACGATACGAGCTTAACGAATCAATACGCAGGTATCAGCATTTTTACTTATTGTACAACACTACAACTAAGTATCAAAATCTAAGCTTGATTCAGACATCCTTGGAATTCTCCAGTGGCCTTAACAGTCTCATTTAGGAGGAAATTGTGATTATGTTCATGCTCTATAGTTGTTCTAGTGAGTTTATAAACATAATGAGGCTCCTTAATTTTTCTAATATGGGATTCTTAACATTATGCAATAACTTTCCTCGTAATCAATTTTTTTTAATTCAG

Coding sequence (CDS)

ATGGAGTCTCTGATTTTGATTCCAGCAAGTAAACACGTTCCAATCACAAATTCTTCTCTATTTTGCTCCGGTTTCTCCATTCCATTCACTTCTAGAAAGCGCGCCATCAATTTCCAACCATGTTGTCCACAGATTCTTCATTGCAATGGCCTTCGACATGCGCAAGCAACTCCGAGGTCTTATATTGGAAATGGACCGCCGTATGGTGAGGAAGATGATGATCCTGAACTACAGGTTGAAAGCCTCAGGGTTCCTGATGATTGGTCGGTTCCTTCCAAGGCATTGGAGGAATCAGAATGGCTTAGGGCTACCTTGCACAAATGGTTAGATGACGAGTACTGTCCAGAGGAAACTAATGTAGAGATAAGCAAGGTTGCAGCAAATTCATATTACAATTCTTTGTTAAAGAAGAGGACAGACCTAGGCGATATTTTGTTGAATATGGCAAGAGAATTGGAATCTATTTCCTATAAGGAAAGTTTTCATGGTGCATTCTCTTCTGCCAATGCAGCAGTGAACTTAATTGCTCAAAGAATAGAGCTGTCTTAA

Protein sequence

MESLILIPASKHVPITNSSLFCSGFSIPFTSRKRAINFQPCCPQILHCNGLRHAQATPRSYIGNGPPYGEEDDDPELQVESLRVPDDWSVPSKALEESEWLRATLHKWLDDEYCPEETNVEISKVAANSYYNSLLKKRTDLGDILLNMARELESISYKESFHGAFSSANAAVNLIAQRIELS
Homology
[Close]
BLAST of Bhi09G000804 vs. TAIR 10
Match: AT4G03150.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 13 growth stages; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 168.7 bits (426), Expect = 4.3e-42
Identity = 84/155 (54.19%), Postives = 113/155 (72.90%), Query Frame = 0 

Query: 26  SIPFTSRKRAINFQPCCPQILHCNGLRHAQATPRSYIGNGPPYGEEDDDPELQVESLRVP 85
           S P T R+R+++F            L+ +Q++            E ++D E  V++L +P
Sbjct: 37  SFPATIRRRSVSFPITSAPKFPSLKLQKSQSS----------IHEGEEDSESAVQALTIP 96

Query: 86  DDWSVPSKALEESEWLRATLHKWLDDEYCPEETNVEISKVAANSYYNSLLKKRTDLGDIL 145
           ++W +PS+A+EESEWLR TLHKWLDDEYCPE TNVEIS+VAA SYY+SLL+K +D+G+IL
Sbjct: 97  EEWLLPSRAIEESEWLRVTLHKWLDDEYCPEPTNVEISEVAAKSYYSSLLEKESDMGEIL 156

Query: 146 LNMARELESISYKESFHGAFSSANAAVNLIAQRIE 181
           L MA++L SISY+ESFHGAF+SANAA+NLI  RIE
Sbjct: 157 LKMAQDLTSISYQESFHGAFTSANAAINLIVDRIE 181

[Close]
BLAST of Bhi09G000804 vs. ExPASy TrEMBL
Match: A0A6J1D9Y8 (uncharacterized protein LOC111018405 OS=Momordica charantia OX=3673 GN=LOC111018405 PE=4 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 7.9e-84
Identity = 155/182 (85.16%), Postives = 167/182 (91.76%), Query Frame = 0 

Query: 1   MESLILIPASKHVPITNSSLFCSGFSIPFTSRKRAINFQPCCPQILHCNGLRHAQATPRS 60
           MESLILIPAS+HV ++NSS FCS F +PFT+ KRAINF+  CPQILH NG+RHAQ TPRS
Sbjct: 1   MESLILIPASRHVSVSNSSGFCSDFYVPFTTGKRAINFRASCPQILHWNGIRHAQTTPRS 60

Query: 61  YIGNGPPYGEEDDDPELQVESLRVPDDWSVPSKALEESEWLRATLHKWLDDEYCPEETNV 120
           Y   GPPYGEEDDDPE+QVESLRVPDDWSVP+KALEESEWLR TLHKWLDDEYCPEETNV
Sbjct: 61  YTRYGPPYGEEDDDPEVQVESLRVPDDWSVPTKALEESEWLRVTLHKWLDDEYCPEETNV 120

Query: 121 EISKVAANSYYNSLLKKRTDLGDILLNMARELESISYKESFHGAFSSANAAVNLIAQRIE 180
           EIS+VAANSYYNSLL+KRTDLGDILL MARELESISYKESFHGAF SANAAVNLIAQ+IE
Sbjct: 121 EISRVAANSYYNSLLEKRTDLGDILLKMARELESISYKESFHGAFXSANAAVNLIAQKIE 180

Query: 181 LS 183
           LS
Sbjct: 181 LS 182

[Close]
BLAST of Bhi09G000804 vs. ExPASy TrEMBL
Match: A0A1S3BS97 (uncharacterized protein LOC103492727 OS=Cucumis melo OX=3656 GN=LOC103492727 PE=4 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 5.7e-82
Identity = 158/182 (86.81%), Postives = 162/182 (89.01%), Query Frame = 0 

Query: 1   MESLILIPASKHVPITNSSLFCSGFSIPFTSRKRAINFQPCCPQILHCNGLRHAQATPRS 60
           MESLILIP SKHV ITNSS F   FS  F+SRKRAINF   CPQ LH NG  HAQ T RS
Sbjct: 1   MESLILIPPSKHVSITNSSAFSFHFSSQFSSRKRAINFPQFCPQTLHSNGRWHAQTTSRS 60

Query: 61  YIGNGPPYGEEDDDPELQVESLRVPDDWSVPSKALEESEWLRATLHKWLDDEYCPEETNV 120
           YI NGPPYGEEDDDPEL+VESLRVPD+WSVPSKALEESEWLR TLHKWLDDEYCPEETNV
Sbjct: 61  YIRNGPPYGEEDDDPELEVESLRVPDEWSVPSKALEESEWLRVTLHKWLDDEYCPEETNV 120

Query: 121 EISKVAANSYYNSLLKKRTDLGDILLNMARELESISYKESFHGAFSSANAAVNLIAQRIE 180
           EISKVAANSYYNSLLKK TDLG+ILLNMARELESISYKESFHGAFSSANAAVNLIAQRIE
Sbjct: 121 EISKVAANSYYNSLLKKTTDLGEILLNMARELESISYKESFHGAFSSANAAVNLIAQRIE 180

Query: 181 LS 183
           LS
Sbjct: 181 LS 182

[Close]
BLAST of Bhi09G000804 vs. ExPASy TrEMBL
Match: A0A5D3D403 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G002040 PE=4 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 5.7e-82
Identity = 158/182 (86.81%), Postives = 162/182 (89.01%), Query Frame = 0 

Query: 1   MESLILIPASKHVPITNSSLFCSGFSIPFTSRKRAINFQPCCPQILHCNGLRHAQATPRS 60
           MESLILIP SKHV ITNSS F   FS  F+SRKRAINF   CPQ LH NG  HAQ T RS
Sbjct: 1   MESLILIPPSKHVSITNSSAFSFHFSSQFSSRKRAINFPQFCPQTLHSNGRWHAQTTSRS 60

Query: 61  YIGNGPPYGEEDDDPELQVESLRVPDDWSVPSKALEESEWLRATLHKWLDDEYCPEETNV 120
           YI NGPPYGEEDDDPEL+VESLRVPD+WSVPSKALEESEWLR TLHKWLDDEYCPEETNV
Sbjct: 61  YIRNGPPYGEEDDDPELEVESLRVPDEWSVPSKALEESEWLRVTLHKWLDDEYCPEETNV 120

Query: 121 EISKVAANSYYNSLLKKRTDLGDILLNMARELESISYKESFHGAFSSANAAVNLIAQRIE 180
           EISKVAANSYYNSLLKK TDLG+ILLNMARELESISYKESFHGAFSSANAAVNLIAQRIE
Sbjct: 121 EISKVAANSYYNSLLKKTTDLGEILLNMARELESISYKESFHGAFSSANAAVNLIAQRIE 180

Query: 181 LS 183
           LS
Sbjct: 181 LS 182

[Close]
BLAST of Bhi09G000804 vs. ExPASy TrEMBL
Match: A0A0A0K5Q9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G390120 PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.2e-79
Identity = 153/182 (84.07%), Postives = 161/182 (88.46%), Query Frame = 0 

Query: 1   MESLILIPASKHVPITNSSLFCSGFSIPFTSRKRAINFQPCCPQILHCNGLRHAQATPRS 60
           MESLILIP +KHV ITNSS+    FS PF SRKRAI F   CPQI+HCN   HAQ T RS
Sbjct: 1   MESLILIPPTKHVSITNSSV----FSFPFISRKRAIKFPQFCPQIIHCNARLHAQTTSRS 60

Query: 61  YIGNGPPYGEEDDDPELQVESLRVPDDWSVPSKALEESEWLRATLHKWLDDEYCPEETNV 120
           YI NGPPYGEEDDDPEL+VESLRVPD+WSVPSKALEESEWLR TLHKWLD+EYCPEETNV
Sbjct: 61  YIRNGPPYGEEDDDPELEVESLRVPDEWSVPSKALEESEWLRVTLHKWLDEEYCPEETNV 120

Query: 121 EISKVAANSYYNSLLKKRTDLGDILLNMARELESISYKESFHGAFSSANAAVNLIAQRIE 180
           +ISKVAA SYYNSLLKK TDLG+ILLNMARELESISYKESFHGAFSSANAAVNLIAQRIE
Sbjct: 121 DISKVAAKSYYNSLLKKTTDLGEILLNMARELESISYKESFHGAFSSANAAVNLIAQRIE 178

Query: 181 LS 183
           LS
Sbjct: 181 LS 178

[Close]
BLAST of Bhi09G000804 vs. ExPASy TrEMBL
Match: A0A6J1H695 (uncharacterized protein LOC111460840 OS=Cucurbita moschata OX=3662 GN=LOC111460840 PE=4 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 2.3e-75
Identity = 147/182 (80.77%), Postives = 160/182 (87.91%), Query Frame = 0 

Query: 1   MESLILIPASKHVPITNSSLFCSGFSIPFTSRKRAINFQPCCPQILHCNGLRHAQATPRS 60
           MESLIL+P+S+HV ITNSS F S F + FTSRKRA+NF+   PQIL CN +   + TPRS
Sbjct: 1   MESLILVPSSRHVSITNSSAFSSDFKVQFTSRKRAMNFRASSPQILPCNVVLE-RLTPRS 60

Query: 61  YIGNGPPYGEEDDDPELQVESLRVPDDWSVPSKALEESEWLRATLHKWLDDEYCPEETNV 120
           YI  GPPYG++DDDPE QVESLRVPD WSVPSKALEESEWLR TLHKWLD+EYCPEETNV
Sbjct: 61  YIRYGPPYGDQDDDPEEQVESLRVPDAWSVPSKALEESEWLRVTLHKWLDEEYCPEETNV 120

Query: 121 EISKVAANSYYNSLLKKRTDLGDILLNMARELESISYKESFHGAFSSANAAVNLIAQRIE 180
           EISKVAA SYYNSLL+KRT+L DILLNMARELESISYKESFHGAFSSANAAVNLIAQRIE
Sbjct: 121 EISKVAAKSYYNSLLEKRTELADILLNMARELESISYKESFHGAFSSANAAVNLIAQRIE 180

Query: 181 LS 183
           LS
Sbjct: 181 LS 181

The following BLAST results are available for this feature:
BLAST of Bhi09G000804 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G03150.14.3e-4254.19unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
BLAST of Bhi09G000804 vs. TAIR 10
Match NameE-valueIdentityDescription
BLAST of Bhi09G000804 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1D9Y87.9e-8485.16uncharacterized protein LOC111018405 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A1S3BS975.7e-8286.81uncharacterized protein LOC103492727 OS=Cucumis melo OX=3656 GN=LOC103492727 PE=... [more]
A0A5D3D4035.7e-8286.81Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A0A0K5Q91.2e-7984.07Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G390120 PE=4 SV=1[more]
A0A6J1H6952.3e-7580.77uncharacterized protein LOC111460840 OS=Cucurbita moschata OX=3662 GN=LOC1114608... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR36776EXPRESSED PROTEINcoord: 48..181

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi09M000804Bhi09M000804mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003855 3-dehydroquinate dehydratase activity
molecular_function GO:0004764 shikimate 3-dehydrogenase (NADP+) activity