Bhi09G000680 (gene) Wax gourd (B227) v1

Overview


Name	Bhi09G000680
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Calvin cycle protein CP12-1
Location	chr9: 18462555 .. 18463549 (+)
RNA-Seq Expression	Bhi09G000680
Synteny	Bhi09G000680

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGACAAATGAACCACCACGTGGACATGTCTCATTGGCTATCATCGGATAAGAAATATGTGCTCGAAAGCGCATGGCGGATTCCAGTTATATATCCCGAAGAAGAAGAACGAAAAAATCTCACTTAATTATTCCTGTCTCTTATACACATCTAGATGTGTATAAGAGACAGACCATGGCGGCCTCAAAGGCGGTGGCTGACTCGCCGAAACTCGAATCGTCGGTCATCCGACCCGCCCGGCTCAACTACCCATGGAAGCAGTGGACCCCCGGTCGGATGTGCGCAGTAGGACCGGTGGCGGCCGCGCCGGACCGGATATCGGAGAAGGTGGTGGAGAGCATAAAGAACGCGGAGGAGACCTGCTCGGAGGATCCGGCGAGCGGGGAGTGCGCGGCGGCGTGGGATGAGGTGGAGGAACTCAGCGCCGCCGCCAGCCACGCACGTGACCGCCTCAAGGATTCGGACCCGCTTGAGGATTTTTGTAAGGATAATCCCGAGACGGAGGAGTGTCGCACGTATGAGGACTGAGTGATTTAATTTCTTTCTATTTCGATTTTATTTGCAACTTTTTTTTTTTGTTGGCTTTTTTTTTCCTTTTGGTAAAGTAGAATGGTTATTTGTGTGTCTTTTTTTGGGCCTTGTTTGTGTTTTTATGTGAAATAAAAATGACTAGAAAATATTTTAGTTCCCACTTATTAAATCATTTATTTATTTTTTAGTTAAAATAAGGTGTGATAATTTGATTCCATACCATGATGGTCAATAGTATCATAAGCTTGGGTTCTTTTTATTTTCAGAGTTAAAGCTAGGAATTCGGATTTACATTAACCAGTTTTGAAAGAAGTTGGTAGCTCATTCATAAGTTGTTTAACTTTATACTCTAATTTTAGAGATATATTGTAATTATTTAAGGCTGGATTTAGGATTACATTTGCAATCATGTGCAATCTCAAAAAGTGATTATAGAGAAGAAAAATGGGATAGATGGCCCAAAAT

mRNA sequence

CGACAAATGAACCACCACGTGGACATGTCTCATTGGCTATCATCGGATAAGAAATATGTGCTCGAAAGCGCATGGCGGATTCCAGTTATATATCCCGAAGAAGAAGAACGAAAAAATCTCACTTAATTATTCCTGTCTCTTATACACATCTAGATGTGTATAAGAGACAGACCATGGCGGCCTCAAAGGCGGTGGCTGACTCGCCGAAACTCGAATCGTCGGTCATCCGACCCGCCCGGCTCAACTACCCATGGAAGCAGTGGACCCCCGGTCGGATGTGCGCAGTAGGACCGGTGGCGGCCGCGCCGGACCGGATATCGGAGAAGGTGGTGGAGAGCATAAAGAACGCGGAGGAGACCTGCTCGGAGGATCCGGCGAGCGGGGAGTGCGCGGCGGCGTGGGATGAGGTGGAGGAACTCAGCGCCGCCGCCAGCCACGCACGTGACCGCCTCAAGGATTCGGACCCGCTTGAGGATTTTTGTAAGGATAATCCCGAGACGGAGGAGTGTCGCACGTATGAGGACTGAGTGATTTAATTTCTTTCTATTTCGATTTTATTTGCAACTTTTTTTTTTTGTTGGCTTTTTTTTTCCTTTTGGTAAAGTAGAATGGTTATTTGTGTGTCTTTTTTTGGGCCTTGTTTGTGTTTTTATGTGAAATAAAAATGACTAGAAAATATTTTAGTTCCCACTTATTAAATCATTTATTTATTTTTTAGTTAAAATAAGGTGTGATAATTTGATTCCATACCATGATGGTCAATAGTATCATAAGCTTGGGTTCTTTTTATTTTCAGAGTTAAAGCTAGGAATTCGGATTTACATTAACCAGTTTTGAAAGAAGTTGGTAGCTCATTCATAAGTTGTTTAACTTTATACTCTAATTTTAGAGATATATTGTAATTATTTAAGGCTGGATTTAGGATTACATTTGCAATCATGTGCAATCTCAAAAAGTGATTATAGAGAAGAAAAATGGGATAGATGGCCCAAAAT

Coding sequence (CDS)

ATGGCGGATTCCAGTTATATATCCCGAAGAAGAAGAACGAAAAAATCTCACTTAATTATTCCTGTCTCTTATACACATCTAGATGTGTATAAGAGACAGACCATGGCGGCCTCAAAGGCGGTGGCTGACTCGCCGAAACTCGAATCGTCGGTCATCCGACCCGCCCGGCTCAACTACCCATGGAAGCAGTGGACCCCCGGTCGGATGTGCGCAGTAGGACCGGTGGCGGCCGCGCCGGACCGGATATCGGAGAAGGTGGTGGAGAGCATAAAGAACGCGGAGGAGACCTGCTCGGAGGATCCGGCGAGCGGGGAGTGCGCGGCGGCGTGGGATGAGGTGGAGGAACTCAGCGCCGCCGCCAGCCACGCACGTGACCGCCTCAAGGATTCGGACCCGCTTGAGGATTTTTGTAAGGATAATCCCGAGACGGAGGAGTGTCGCACGTATGAGGACTGA

Protein sequence

MADSSYISRRRRTKKSHLIIPVSYTHLDVYKRQTMAASKAVADSPKLESSVIRPARLNYPWKQWTPGRMCAVGPVAAAPDRISEKVVESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLKDSDPLEDFCKDNPETEECRTYED

Homology

[Close]

BLAST of Bhi09G000680 vs. TAIR 10
Match: AT2G47400.1 (CP12 domain-containing protein 1 )

HSP 1 Score: 124.8 bits (312), Expect = 5.9e-29
Identity = 69/124 (55.65%), Postives = 89/124 (71.77%), Query Frame = 0


Query: 34  TMAASKAVADSPKL----ESSVIRPARLNYPWKQWTPGRMCAVGPVAAAPDRISEKVVES 93
           T+AA+     +P++     + V+ P RLNYPWK  +  RM  V   A +   ISEKV +S
Sbjct: 3   TIAAAGLNVATPRVVVRPVARVLGPVRLNYPWKFGSMKRMVVV--KATSEGEISEKVEKS 62

Query: 94  IKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLK--DSDPLEDFCKDNPETEECR 152
           I+ A+ETC++DP SGEC AAWDEVEELSAAASHARD+ K   SDPLE++C DNPET+ECR
Sbjct: 63  IQEAKETCADDPVSGECVAAWDEVEELSAAASHARDKKKAGGSDPLEEYCNDNPETDECR 122

[Close]

BLAST of Bhi09G000680 vs. TAIR 10
Match: AT3G62410.1 (CP12 domain-containing protein 2 )

HSP 1 Score: 115.2 bits (287), Expect = 4.7e-26
Identity = 63/103 (61.17%), Postives = 75/103 (72.82%), Query Frame = 0


Query: 54  PARLNYPWK--QWTPGRMCAVGPVAAAPD-RISEKVVESIKNAEETCSEDPASGECAAAW 113
           P  LN  W     T  RM  + P+ AAP+  IS+ V +SIK A+ETC+ DP SGEC AAW
Sbjct: 29  PVHLNNSWNLGSRTTNRMMKLQPIKAAPEGGISDVVEKSIKEAQETCAGDPVSGECVAAW 88

Query: 114 DEVEELSAAASHARDRLK--DSDPLEDFCKDNPETEECRTYED 152
           DEVEELSAAASHARD+ K   SDPLE++CKDNPET ECRTY++
Sbjct: 89  DEVEELSAAASHARDKKKADGSDPLEEYCKDNPETNECRTYDN 131

[Close]

BLAST of Bhi09G000680 vs. TAIR 10
Match: AT1G76560.1 (CP12 domain-containing protein 3 )

HSP 1 Score: 76.6 bits (187), Expect = 1.9e-14
Identity = 38/74 (51.35%), Postives = 51/74 (68.92%), Query Frame = 0


Query: 80  DRISEKVVESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLK--DSDPLEDFC 139
           +++SE + E +K A E C  +  S EC  AWDEVEE+S A +  R +LK  + DPLE FC
Sbjct: 61  EKLSEMIEEKVKEATEVCEAEEMSEECRVAWDEVEEVSQARADLRIKLKLLNQDPLESFC 120

Query: 140 KDNPETEECRTYED 152
           ++NPET+ECR YED
Sbjct: 121 QENPETDECRIYED 134

[Close]

BLAST of Bhi09G000680 vs. ExPASy Swiss-Prot
Match: O22914 (Calvin cycle protein CP12-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CP12-1 PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 8.4e-28
Identity = 69/124 (55.65%), Postives = 89/124 (71.77%), Query Frame = 0


Query: 34  TMAASKAVADSPKL----ESSVIRPARLNYPWKQWTPGRMCAVGPVAAAPDRISEKVVES 93
           T+AA+     +P++     + V+ P RLNYPWK  +  RM  V   A +   ISEKV +S
Sbjct: 3   TIAAAGLNVATPRVVVRPVARVLGPVRLNYPWKFGSMKRMVVV--KATSEGEISEKVEKS 62

Query: 94  IKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLK--DSDPLEDFCKDNPETEECR 152
           I+ A+ETC++DP SGEC AAWDEVEELSAAASHARD+ K   SDPLE++C DNPET+ECR
Sbjct: 63  IQEAKETCADDPVSGECVAAWDEVEELSAAASHARDKKKAGGSDPLEEYCNDNPETDECR 122

[Close]

BLAST of Bhi09G000680 vs. ExPASy Swiss-Prot
Match: Q9LZP9 (Calvin cycle protein CP12-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CP12-2 PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 6.6e-25
Identity = 63/103 (61.17%), Postives = 75/103 (72.82%), Query Frame = 0


Query: 54  PARLNYPWK--QWTPGRMCAVGPVAAAPD-RISEKVVESIKNAEETCSEDPASGECAAAW 113
           P  LN  W     T  RM  + P+ AAP+  IS+ V +SIK A+ETC+ DP SGEC AAW
Sbjct: 29  PVHLNNSWNLGSRTTNRMMKLQPIKAAPEGGISDVVEKSIKEAQETCAGDPVSGECVAAW 88

Query: 114 DEVEELSAAASHARDRLK--DSDPLEDFCKDNPETEECRTYED 152
           DEVEELSAAASHARD+ K   SDPLE++CKDNPET ECRTY++
Sbjct: 89  DEVEELSAAASHARDKKKADGSDPLEEYCKDNPETNECRTYDN 131

[Close]

BLAST of Bhi09G000680 vs. ExPASy Swiss-Prot
Match: Q9C9K2 (Calvin cycle protein CP12-3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CP12-3 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 2.6e-13
Identity = 38/74 (51.35%), Postives = 51/74 (68.92%), Query Frame = 0


Query: 80  DRISEKVVESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLK--DSDPLEDFC 139
           +++SE + E +K A E C  +  S EC  AWDEVEE+S A +  R +LK  + DPLE FC
Sbjct: 61  EKLSEMIEEKVKEATEVCEAEEMSEECRVAWDEVEEVSQARADLRIKLKLLNQDPLESFC 120

Query: 140 KDNPETEECRTYED 152
           ++NPET+ECR YED
Sbjct: 121 QENPETDECRIYED 134

[Close]

BLAST of Bhi09G000680 vs. ExPASy Swiss-Prot
Match: A6Q0K5 (Calvin cycle protein CP12, chloroplastic OS=Chlamydomonas reinhardtii OX=3055 GN=CP12 PE=1 SV=2)

HSP 1 Score: 73.9 bits (180), Expect = 1.7e-12
Identity = 38/74 (51.35%), Postives = 53/74 (71.62%), Query Frame = 0


Query: 82  ISEKVVESIKNAEETCSEDPASGECAAAWDEVEELSAAASHARDRLKD----SDPLEDFC 141
           +++KV +++K AE+ C++   S +CA AWD VEELSAA SH +D +K     +DPLE FC
Sbjct: 35  LNKKVQDAVKEAEDACAKG-TSADCAVAWDTVEELSAAVSHKKDAVKADVTLTDPLEAFC 94

Query: 142 KDNPETEECRTYED 152
           KD P+ +ECR YED
Sbjct: 95  KDAPDADECRVYED 107

[Close]

BLAST of Bhi09G000680 vs. ExPASy TrEMBL
Match: A0A6J1JWF5 (calvin cycle protein CP12-1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111488107 PE=4 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 1.6e-53
Identity = 108/118 (91.53%), Postives = 113/118 (95.76%), Query Frame = 0


Query: 34  TMAASKAVADSPKLESSVIRPARLNYPWKQWTPGRMCAVGPVAAAPDRISEKVVESIKNA 93
           TMA SKAVADSPKLES V+R ARLNYPWK+W+  R+CAVGPV+AAPDRISEKVVESIKNA
Sbjct: 8   TMAVSKAVADSPKLESPVLRSARLNYPWKRWSAVRLCAVGPVSAAPDRISEKVVESIKNA 67

Query: 94  EETCSEDPASGECAAAWDEVEELSAAASHARDRLKDSDPLEDFCKDNPETEECRTYED 152
           EETCSEDPASGECAAAWDEVEELSAAASHARDRLKDSDPLEDFCKDNPETEECRTYED
Sbjct: 68  EETCSEDPASGECAAAWDEVEELSAAASHARDRLKDSDPLEDFCKDNPETEECRTYED 125

[Close]

BLAST of Bhi09G000680 vs. ExPASy TrEMBL
Match: A0A6J1GQL5 (calvin cycle protein CP12-1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111456202 PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.9e-51
Identity = 106/118 (89.83%), Postives = 112/118 (94.92%), Query Frame = 0


Query: 34  TMAASKAVADSPKLESSVIRPARLNYPWKQWTPGRMCAVGPVAAAPDRISEKVVESIKNA 93
           TMA SKAVADSPKLES+V+R ARL YPWK+W+  R+ AVGPV+AAPDRISEKVVESIKNA
Sbjct: 8   TMAVSKAVADSPKLESTVLRSARLYYPWKRWSAVRLRAVGPVSAAPDRISEKVVESIKNA 67

Query: 94  EETCSEDPASGECAAAWDEVEELSAAASHARDRLKDSDPLEDFCKDNPETEECRTYED 152
           EETCSEDPASGECAAAWDEVEELSAAASHARDRLKDSDPLEDFCKDNPETEECRTYED
Sbjct: 68  EETCSEDPASGECAAAWDEVEELSAAASHARDRLKDSDPLEDFCKDNPETEECRTYED 125

[Close]

BLAST of Bhi09G000680 vs. ExPASy TrEMBL
Match: A0A5A7TUE8 (Calvin cycle protein CP12-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001700 PE=4 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 9.5e-51
Identity = 104/117 (88.89%), Postives = 110/117 (94.02%), Query Frame = 0


Query: 35  MAASKAVADSPKLESSVIRPARLNYPWKQWTPGRMCAVGPVAAAPDRISEKVVESIKNAE 94
           MAA+KAV DSPK+ES V R +RL+YPWK+WT GRM AVGPVAAAPDRISEKVVESIKNAE
Sbjct: 1   MAATKAVTDSPKIESPVHRSSRLSYPWKRWTSGRMSAVGPVAAAPDRISEKVVESIKNAE 60

Query: 95  ETCSEDPASGECAAAWDEVEELSAAASHARDRLKDSDPLEDFCKDNPETEECRTYED 152
            TCSEDPASGECAAAWDEVEELSAAASHARDRLKD+DPLEDFCKDNPETEECRTYED
Sbjct: 61  VTCSEDPASGECAAAWDEVEELSAAASHARDRLKDADPLEDFCKDNPETEECRTYED 117

[Close]

BLAST of Bhi09G000680 vs. ExPASy TrEMBL
Match: A0A1S3BSD2 (calvin cycle protein CP12-1, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492757 PE=4 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 9.5e-51
Identity = 104/117 (88.89%), Postives = 110/117 (94.02%), Query Frame = 0


Query: 35  MAASKAVADSPKLESSVIRPARLNYPWKQWTPGRMCAVGPVAAAPDRISEKVVESIKNAE 94
           MAA+KAV DSPK+ES V R +RL+YPWK+WT GRM AVGPVAAAPDRISEKVVESIKNAE
Sbjct: 1   MAATKAVTDSPKIESPVHRSSRLSYPWKRWTSGRMSAVGPVAAAPDRISEKVVESIKNAE 60

Query: 95  ETCSEDPASGECAAAWDEVEELSAAASHARDRLKDSDPLEDFCKDNPETEECRTYED 152
            TCSEDPASGECAAAWDEVEELSAAASHARDRLKD+DPLEDFCKDNPETEECRTYED
Sbjct: 61  VTCSEDPASGECAAAWDEVEELSAAASHARDRLKDADPLEDFCKDNPETEECRTYED 117

[Close]

BLAST of Bhi09G000680 vs. ExPASy TrEMBL
Match: A0A0A0K832 (CP12 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G407670 PE=4 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 4.0e-49
Identity = 102/118 (86.44%), Postives = 108/118 (91.53%), Query Frame = 0


Query: 34  TMAASKAVADSPKLESSVIRPARLNYPWKQWTPGRMCAVGPVAAAPDRISEKVVESIKNA 93
           +MAASKAV DSPK+E  V+R +RL+YPWK+WT GRM AVG VAAAPDRISEKVVESIKNA
Sbjct: 64  SMAASKAVTDSPKIELPVLRSSRLSYPWKRWTSGRMSAVGTVAAAPDRISEKVVESIKNA 123

Query: 94  EETCSEDPASGECAAAWDEVEELSAAASHARDRLKDSDPLEDFCKDNPETEECRTYED 152
           E TCSEDPASGECAAAWDEVEELSAAASHARDRL  SDPLEDFCKDNPETEECRTYED
Sbjct: 124 EVTCSEDPASGECAAAWDEVEELSAAASHARDRLSHSDPLEDFCKDNPETEECRTYED 181

The following BLAST results are available for this feature:

BLAST of Bhi09G000680 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G47400.1	5.9e-29	55.65	CP12 domain-containing protein 1	[more]
AT3G62410.1	4.7e-26	61.17	CP12 domain-containing protein 2	[more]
AT1G76560.1	1.9e-14	51.35	CP12 domain-containing protein 3	[more]

BLAST of Bhi09G000680 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22914	8.4e-28	55.65	Calvin cycle protein CP12-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CP...	[more]
Q9LZP9	6.6e-25	61.17	Calvin cycle protein CP12-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CP...	[more]
Q9C9K2	2.6e-13	51.35	Calvin cycle protein CP12-3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CP...	[more]
A6Q0K5	1.7e-12	51.35	Calvin cycle protein CP12, chloroplastic OS=Chlamydomonas reinhardtii OX=3055 GN...	[more]

BLAST of Bhi09G000680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JWF5	1.6e-53	91.53	calvin cycle protein CP12-1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1GQL5	1.9e-51	89.83	calvin cycle protein CP12-1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN...	[more]
A0A5A7TUE8	9.5e-51	88.89	Calvin cycle protein CP12-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3BSD2	9.5e-51	88.89	calvin cycle protein CP12-1, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A0A0K832	4.0e-49	86.44	CP12 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G407670 PE=4 S...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003823	Domain of unknown function CP12	SMART	SM01093	CP12_2	coord: 82..151 e-value: 2.2E-28 score: 110.3
IPR003823	Domain of unknown function CP12	PFAM	PF02672	CP12	coord: 82..150 e-value: 2.9E-26 score: 91.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 121..151
None	No IPR available	PANTHER	PTHR33921:SF15	CALVIN CYCLE PROTEIN CP12-2, CHLOROPLASTIC	coord: 34..151
IPR039314	Calvin cycle protein CP12-like	PANTHER	PTHR33921	CALVIN CYCLE PROTEIN CP12-2, CHLOROPLASTIC	coord: 34..151

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi09M000680	Bhi09M000680	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004674	protein serine/threonine kinase activity