Bhi09G000297 (gene) Wax gourd (B227) v1

Overview
NameBhi09G000297
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionervatamin-B-like
Locationchr9: 7680293 .. 7681640 (+)
RNA-Seq ExpressionBhi09G000297
SyntenyBhi09G000297
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATTTCTCATTGTTCCTCTTGTTTTGATTGTTCTAACTTTTGGATTATGTGAGAGCTTTAAATTCGAAGGGAAAGAGTTAGAATCCGAAGAAAGTCTATGGCATCTATATAAGAGATGGAGTAGCCACCATAGAATCTTAAGGAATGGAAGGGAGATGCACAATCGTTTCAAGGTGTTCAAAGAAAATGCAAAGTATGTGTTCAAAGTGAACCAGATGAACAAATCATTAAAGTTGAAGTTAAATCAGTTCGCTGATATGTCCGATGATGAGTTTATTAATACACATGTTAGCTCCAATATTACCTACTACAAAAACTTACATGCCAAGAAAATAGAAGCTCTTGGTGGTCGTGTTGGTGGGTTCATGTATGAGCACGTTGAGGATCTTCCATCTTCAATCGATTGGAGGAAAAAAGGAGCCGTCACTGACATAAAAAACCAAGGCCGTTGTGGTAATAATTAATTTTGAATTATGGCTTTGTAAAATTCCTAATTATTCAACCTCGTACATAATAAATTTTAAAATATCCTTGTAGGAAGTTGTTGGGCATTTGCAGCGGTGGCTGTGGTTGAAGGAATTAACCAAATCAAAACCAACAAGCTAGTATCTCTCTCAGAGCAAGAGGTGGTCGATTGTGATTATAAAGACGGAGGTTGCGGTGGAGGATTTTATGACTCAGCTTTTGAATTCATGATGGAAAACAATGGGATCACAATTGAGGAAAATTATCCCTATTATGCCGAAAACAGATATTGCCTCGCACAAAAAGTGAGTTTGCTTTACATAATTAGAAAAAAAAATTCTATGTCTATGCAATTAAATTCATCATTATGTATGTCTATGCTATTAAATTCATCATTATGTTTGATTTAACAGGGTAATAAGAGAGTGACAATTGATGGATATGAGAATGTACCTCCAAATAATGAGAATGCTTTGAAGAAAGCTGTAGCACACCAACCGGTAGCAGTATCCATAGCCGCCAGTGGACGACGTTTTCGATATTACTCAGAGGCAAGTAGTTTTGTTTGATTGATTGTAACTATGATTGATTGTAACTTATTTGTGTAGAACAGTTTTAATGTTCAAGCATTGTTAAAAGTGAATTTGTAATTTGCAGGGAATGTTTACTGAAAATGACTATTGTGGAGATAGAATTGATCACACAGTGGTGGTAGTTGGATACGGAACAGAGGAAGACGGAACAGATTATTGGATCATAAAGAACTCGTGGGGAACTAACTGGGGATTGGAAGGTTATATGAAGATGCAACGAGGAGCACAACAACCGGAAGGTGTATGCGGATTGGCAATGAATCCATCCTATCCCATAAAGTACTAG

mRNA sequence

ATGAAATTTCTCATTGTTCCTCTTGTTTTGATTGTTCTAACTTTTGGATTATGTGAGAGCTTTAAATTCGAAGGGAAAGAGTTAGAATCCGAAGAAAGTCTATGGCATCTATATAAGAGATGGAGTAGCCACCATAGAATCTTAAGGAATGGAAGGGAGATGCACAATCGTTTCAAGGTGTTCAAAGAAAATGCAAAGTATGTGTTCAAAGTGAACCAGATGAACAAATCATTAAAGTTGAAGTTAAATCAGTTCGCTGATATGTCCGATGATGAGTTTATTAATACACATGTTAGCTCCAATATTACCTACTACAAAAACTTACATGCCAAGAAAATAGAAGCTCTTGGTGGTCGTGTTGGTGGGTTCATGTATGAGCACGTTGAGGATCTTCCATCTTCAATCGATTGGAGGAAAAAAGGAGCCGTCACTGACATAAAAAACCAAGGCCGTTGTGGAAGTTGTTGGGCATTTGCAGCGGTGGCTGTGGTTGAAGGAATTAACCAAATCAAAACCAACAAGCTAGTATCTCTCTCAGAGCAAGAGGTGGTCGATTGTGATTATAAAGACGGAGGTTGCGGTGGAGGATTTTATGACTCAGCTTTTGAATTCATGATGGAAAACAATGGGATCACAATTGAGGAAAATTATCCCTATTATGCCGAAAACAGATATTGCCTCGCACAAAAAGGTAATAAGAGAGTGACAATTGATGGATATGAGAATGTACCTCCAAATAATGAGAATGCTTTGAAGAAAGCTGTAGCACACCAACCGGTAGCAGTATCCATAGCCGCCAGTGGACGACGTTTTCGATATTACTCAGAGGCAAGTAGTTTTGGAATGTTTACTGAAAATGACTATTGTGGAGATAGAATTGATCACACAGTGGTGGTAGTTGGATACGGAACAGAGGAAGACGGAACAGATTATTGGATCATAAAGAACTCGTGGGGAACTAACTGGGGATTGGAAGGTTATATGAAGATGCAACGAGGAGCACAACAACCGGAAGGTGTATGCGGATTGGCAATGAATCCATCCTATCCCATAAAGTACTAG

Coding sequence (CDS)

ATGAAATTTCTCATTGTTCCTCTTGTTTTGATTGTTCTAACTTTTGGATTATGTGAGAGCTTTAAATTCGAAGGGAAAGAGTTAGAATCCGAAGAAAGTCTATGGCATCTATATAAGAGATGGAGTAGCCACCATAGAATCTTAAGGAATGGAAGGGAGATGCACAATCGTTTCAAGGTGTTCAAAGAAAATGCAAAGTATGTGTTCAAAGTGAACCAGATGAACAAATCATTAAAGTTGAAGTTAAATCAGTTCGCTGATATGTCCGATGATGAGTTTATTAATACACATGTTAGCTCCAATATTACCTACTACAAAAACTTACATGCCAAGAAAATAGAAGCTCTTGGTGGTCGTGTTGGTGGGTTCATGTATGAGCACGTTGAGGATCTTCCATCTTCAATCGATTGGAGGAAAAAAGGAGCCGTCACTGACATAAAAAACCAAGGCCGTTGTGGAAGTTGTTGGGCATTTGCAGCGGTGGCTGTGGTTGAAGGAATTAACCAAATCAAAACCAACAAGCTAGTATCTCTCTCAGAGCAAGAGGTGGTCGATTGTGATTATAAAGACGGAGGTTGCGGTGGAGGATTTTATGACTCAGCTTTTGAATTCATGATGGAAAACAATGGGATCACAATTGAGGAAAATTATCCCTATTATGCCGAAAACAGATATTGCCTCGCACAAAAAGGTAATAAGAGAGTGACAATTGATGGATATGAGAATGTACCTCCAAATAATGAGAATGCTTTGAAGAAAGCTGTAGCACACCAACCGGTAGCAGTATCCATAGCCGCCAGTGGACGACGTTTTCGATATTACTCAGAGGCAAGTAGTTTTGGAATGTTTACTGAAAATGACTATTGTGGAGATAGAATTGATCACACAGTGGTGGTAGTTGGATACGGAACAGAGGAAGACGGAACAGATTATTGGATCATAAAGAACTCGTGGGGAACTAACTGGGGATTGGAAGGTTATATGAAGATGCAACGAGGAGCACAACAACCGGAAGGTGTATGCGGATTGGCAATGAATCCATCCTATCCCATAAAGTACTAG

Protein sequence

MKFLIVPLVLIVLTFGLCESFKFEGKELESEESLWHLYKRWSSHHRILRNGREMHNRFKVFKENAKYVFKVNQMNKSLKLKLNQFADMSDDEFINTHVSSNITYYKNLHAKKIEALGGRVGGFMYEHVEDLPSSIDWRKKGAVTDIKNQGRCGSCWAFAAVAVVEGINQIKTNKLVSLSEQEVVDCDYKDGGCGGGFYDSAFEFMMENNGITIEENYPYYAENRYCLAQKGNKRVTIDGYENVPPNNENALKKAVAHQPVAVSIAASGRRFRYYSEASSFGMFTENDYCGDRIDHTVVVVGYGTEEDGTDYWIIKNSWGTNWGLEGYMKMQRGAQQPEGVCGLAMNPSYPIKY
Homology
BLAST of Bhi09G000297 vs. TAIR 10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 355.5 bits (911), Expect = 4.8e-98
Identity = 180/354 (50.85%), Postives = 237/354 (66.95%), Query Frame = 0

Query: 1   MKFLIVPLVLIVLTFGLCESFKFEGKELESEESLWHLYKRWSSHHRILRNGREMHNRFKV 60
           MK  IV  + +++     +   F  K++ESE SLW LY+RW SHH + R+  E   RF V
Sbjct: 1   MKRFIVLALCMLMVLETTKGLDFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFNV 60

Query: 61  FKENAKYVFKVNQMNKSLKLKLNQFADMSDDEFINTHVSSNITYYKNLHAKKIEALGGRV 120
           FK N K++ + N+ +KS KLKLN+F DM+ +EF  T+  SNI +++    +K        
Sbjct: 61  FKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEK-----KAT 120

Query: 121 GGFMYEHVEDLPSSIDWRKKGAVTDIKNQGRCGSCWAFAAVAVVEGINQIKTNKLVSLSE 180
             FMY +V  LP+S+DWRK GAVT +KNQG+CGSCWAF+ V  VEGINQI+T KL SLSE
Sbjct: 121 KSFMYANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTSLSE 180

Query: 181 QEVVDCD-YKDGGCGGGFYDSAFEFMMENNGITIEENYPYYAENRYCLAQKGNKR-VTID 240
           QE+VDCD  ++ GC GG  D AFEF+ E  G+T E  YPY A +  C   K N   V+ID
Sbjct: 181 QELVDCDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSID 240

Query: 241 GYENVPPNNENALKKAVAHQPVAVSIAASGRRFRYYSEASSFGMFTENDYCGDRIDHTVV 300
           G+E+VP N+E+ L KAVA+QPV+V+I A G  F++YSE    G+FT    CG  ++H V 
Sbjct: 241 GHEDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSE----GVFTGR--CGTELNHGVA 300

Query: 301 VVGYGTEEDGTDYWIIKNSWGTNWGLEGYMKMQRGAQQPEGVCGLAMNPSYPIK 353
           VVGYGT  DGT YWI+KNSWG  WG +GY++MQRG +  EG+CG+AM  SYP+K
Sbjct: 301 VVGYGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIAMEASYPLK 343

BLAST of Bhi09G000297 vs. TAIR 10
Match: AT3G48340.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 350.1 bits (897), Expect = 2.0e-96
Identity = 184/353 (52.12%), Postives = 242/353 (68.56%), Query Frame = 0

Query: 2   KFLIVPLVLIVLTFGLCESFKFEGKELESEESLWHLYKRWSSHHRILRNGREMHNRFKVF 61
           K L++ L  +V+    C  F ++ KE+ESEE L  LY RW SHH + R+  E   RF VF
Sbjct: 3   KLLLIFLFSLVILQTAC-GFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFNVF 62

Query: 62  KENAKYVFKVNQMNKSLKLKLNQFADMSDDEFINTHVSSNITYYKNLHAKKIEALGGRVG 121
           + N  +V   N+ N+S KLKLN+FAD++ +EF N +  SNI +++ L   K    G +  
Sbjct: 63  RHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPK---RGSKQF 122

Query: 122 GFMYEHVEDLPSSIDWRKKGAVTDIKNQGRCGSCWAFAAVAVVEGINQIKTNKLVSLSEQ 181
            + +E++  LPSS+DWRKKGAVT+IKNQG+CGSCWAF+ VA VEGIN+IKTNKLVSLSEQ
Sbjct: 123 MYDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQ 182

Query: 182 EVVDCDYKDG-GCGGGFYDSAFEFMMENNGITIEENYPYYAENRYCLAQKGN-KRVTIDG 241
           E+VDCD K   GC GG  + AFEF+ +N GIT E++YPY   +  C A K N   VTIDG
Sbjct: 183 ELVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDG 242

Query: 242 YENVPPNNENALKKAVAHQPVAVSIAASGRRFRYYSEASSFGMFTENDYCGDRIDHTVVV 301
           +E+VP N+ENAL KAVA+QPV+V+I A    F++YSE    G+FT +  CG  ++H V  
Sbjct: 243 HEDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSE----GVFTGS--CGTELNHGVAA 302

Query: 302 VGYGTEEDGTDYWIIKNSWGTNWGLEGYMKMQRGAQQPEGVCGLAMNPSYPIK 353
           VGYG+E  G  YWI++NSWG  WG  GY+K++R   +PEG CG+AM  SYPIK
Sbjct: 303 VGYGSER-GKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of Bhi09G000297 vs. TAIR 10
Match: AT3G48350.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 348.2 bits (892), Expect = 7.7e-96
Identity = 173/355 (48.73%), Postives = 238/355 (67.04%), Query Frame = 0

Query: 1   MKFLIVPLVLIVLTFGLCESFKFEGKELESEESLWHLYKRWSSHHRILRNGREMHNRFKV 60
           MK   + L+  +      + F F+ KELE+EE++W LY+RW  HH + R   E   RF V
Sbjct: 1   MKLFFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNV 60

Query: 61  FKENAKYVFKVNQMNKSLKLKLNQFADMSDDEFINTHVSSNITYYKNLHAKKIEALGGRV 120
           F+ N  +V + N+ NK  KLK+N+FAD++  EF +++  SN+ +++ L   K  +     
Sbjct: 61  FRHNVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGS----- 120

Query: 121 GGFMYEHVEDLPSSIDWRKKGAVTDIKNQGRCGSCWAFAAVAVVEGINQIKTNKLVSLSE 180
           GGFMYE+V  +PSS+DWR+KGAVT++KNQ  CGSCWAF+ VA VEGIN+I+TNKLVSLSE
Sbjct: 121 GGFMYENVTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSE 180

Query: 181 QEVVDCDYKDG-GCGGGFYDSAFEFMMENNGITIEENYPYYAEN-RYCLAQK-GNKRVTI 240
           QE+VDCD ++  GC GG  + AFEF+  N GI  EE YPY + + ++C A   G + VTI
Sbjct: 181 QELVDCDTEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTI 240

Query: 241 DGYENVPPNNENALKKAVAHQPVAVSIAASGRRFRYYSEASSFGMFTENDYCGDRIDHTV 300
           DG+E+VP N+E  L KAVAHQPV+V+I A    F+ YSE    G       CG +++H V
Sbjct: 241 DGHEHVPENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFIG------ECGTQLNHGV 300

Query: 301 VVVGYGTEEDGTDYWIIKNSWGTNWGLEGYMKMQRGAQQPEGVCGLAMNPSYPIK 353
           V+VGYG  ++GT YWI++NSWG  WG  GY++++RG  + EG CG+AM  SYP K
Sbjct: 301 VIVGYGETKNGTKYWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

BLAST of Bhi09G000297 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 294.7 bits (753), Expect = 1.0e-79
Identity = 163/358 (45.53%), Postives = 233/358 (65.08%), Query Frame = 0

Query: 1   MKFLIVPLVLIVLTFGLCESFKFEGKELESEESLWHLYKRWSSHHRILRNG-REMHNRFK 60
           +  LI  ++LI L+ G   + +    E E+      +Y+RW   +R   NG  E   RF+
Sbjct: 10  LALLIFSVLLISLSLGSVTATETTRNEAEAR----RMYERWLVENRKNYNGLGEKERRFE 69

Query: 61  VFKENAKYVFKVNQM-NKSLKLKLNQFADMSDDEFINTHVSSNITYYKNLHAKKIEALGG 120
           +FK+N K+V + + + N++ ++ L +FAD+++DEF   ++ S       +   ++   G 
Sbjct: 70  IFKDNLKFVEEHSSIPNRTYEVGLTRFADLTNDEFRAIYLRS------KMERTRVPVKGE 129

Query: 121 RVGGFMYEHVEDLPSSIDWRKKGAVTDIKNQGRCGSCWAFAAVAVVEGINQIKTNKLVSL 180
           +   ++Y+  + LP +IDWR KGAV  +K+QG CGSCWAF+A+  VEGINQIKT +L+SL
Sbjct: 130 K---YLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSCWAFSAIGAVEGINQIKTGELISL 189

Query: 181 SEQEVVDCD--YKDGGCGGGFYDSAFEFMMENNGITIEENYPYYAEN-RYCLAQKGNKR- 240
           SEQE+VDCD  Y D GCGGG  D AF+F++EN GI  EE+YPY A +   C + K N R 
Sbjct: 190 SEQELVDCDTSYND-GCGGGLMDYAFKFIIENGGIDTEEDYPYIATDVNVCNSDKKNTRV 249

Query: 241 VTIDGYENVPPNNENALKKAVAHQPVAVSIAASGRRFRYYSEASSFGMFTENDYCGDRID 300
           VTIDGYE+VP N+E +LKKA+A+QP++V+I A GR F+ Y+     G+FT    CG  +D
Sbjct: 250 VTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQLYTS----GVFTGT--CGTSLD 309

Query: 301 HTVVVVGYGTEEDGTDYWIIKNSWGTNWGLEGYMKMQRGAQQPEGVCGLAMNPSYPIK 353
           H VV VGYG+ E G DYWI++NSWG+NWG  GY K++R  ++  G CG+AM  SYP K
Sbjct: 310 HGVVAVGYGS-EGGQDYWIVRNSWGSNWGESGYFKLERNIKESSGKCGVAMMASYPTK 346

BLAST of Bhi09G000297 vs. TAIR 10
Match: AT5G45890.1 (senescence-associated gene 12 )

HSP 1 Score: 290.8 bits (743), Expect = 1.5e-78
Identity = 158/349 (45.27%), Postives = 217/349 (62.18%), Query Frame = 0

Query: 11  IVLTFGLCESFKFE---GKELESEESLWHLYKRW-SSHHRILRNGREMHNRFKVFKENAK 70
           I L   +  SF F     + L++E  +   +  W + H R+  + +E +NR+ VFK N +
Sbjct: 8   IFLFVAIFSSFCFSITLSRPLDNELIMQKRHIEWMTKHGRVYADVKEENNRYVVFKNNVE 67

Query: 71  YVFKVNQM--NKSLKLKLNQFADMSDDEFINTHVSSNITYYKNLHAKKIEALGGRVGGFM 130
            +  +N +   ++ KL +NQFAD+++DEF      S  T +K + A   ++   ++  F 
Sbjct: 68  RIEHLNSIPAGRTFKLAVNQFADLTNDEF-----RSMYTGFKGVSALSSQS-QTKMSPFR 127

Query: 131 YEHVED--LPSSIDWRKKGAVTDIKNQGRCGSCWAFAAVAVVEGINQIKTNKLVSLSEQE 190
           Y++V    LP S+DWRKKGAVT IKNQG CG CWAF+AVA +EG  QIK  KL+SLSEQ+
Sbjct: 128 YQNVSSGALPVSVDWRKKGAVTPIKNQGSCGCCWAFSAVAAIEGATQIKKGKLISLSEQQ 187

Query: 191 VVDCDYKDGGCGGGFYDSAFEFMMENNGITIEENYPYYAENRYCLAQKGNKRVT-IDGYE 250
           +VDCD  D GC GG  D+AFE +    G+T E NYPY  E+  C ++K N + T I GYE
Sbjct: 188 LVDCDTNDFGCEGGLMDTAFEHIKATGGLTTESNYPYKGEDATCNSKKTNPKATSITGYE 247

Query: 251 NVPPNNENALKKAVAHQPVAVSIAASGRRFRYYSEASSFGMFTENDYCGDRIDHTVVVVG 310
           +VP N+E AL KAVAHQPV+V I   G  F++YS     G+FT    C   +DH V  +G
Sbjct: 248 DVPVNDEQALMKAVAHQPVSVGIEGGGFDFQFYSS----GVFTGE--CTTYLDHAVTAIG 307

Query: 311 YGTEEDGTDYWIIKNSWGTNWGLEGYMKMQRGAQQPEGVCGLAMNPSYP 351
           YG   +G+ YWIIKNSWGT WG  GYM++Q+  +  +G+CGLAM  SYP
Sbjct: 308 YGESTNGSKYWIIKNSWGTKWGESGYMRIQKDVKDKQGLCGLAMKASYP 344

BLAST of Bhi09G000297 vs. ExPASy Swiss-Prot
Match: O65039 (Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 9.8e-104
Identity = 195/354 (55.08%), Postives = 247/354 (69.77%), Query Frame = 0

Query: 2   KFLIVPLVLIVLTFGLCESFKFEGKELESEESLWHLYKRWSSHHRILRNGREMHNRFKVF 61
           KF+++ L L  L   + ESF F  KELESEESLW LY+RW SHH + R+  E   RF VF
Sbjct: 3   KFILLALSL-ALVLAITESFDFHEKELESEESLWGLYERWRSHHTVSRSLHEKQKRFNVF 62

Query: 62  KENAKYVFKVNQMNKSLKLKLNQFADMSDDEFINTHVSSNITYYKNLHAKKIEALGGRVG 121
           K NA +V   N+M+K  KLKLN+FADM++ EF NT+  S + +++          G R  
Sbjct: 63  KHNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNTYSGSKVKHHRMFRG------GPRGN 122

Query: 122 G-FMYEHVEDLPSSIDWRKKGAVTDIKNQGRCGSCWAFAAVAVVEGINQIKTNKLVSLSE 181
           G FMYE V+ +P+S+DWRKKGAVT +K+QG+CGSCWAF+ +  VEGINQIKTNKLVSLSE
Sbjct: 123 GTFMYEKVDTVPASVDWRKKGAVTSVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSE 182

Query: 182 QEVVDCDY-KDGGCGGGFYDSAFEFMMENNGITIEENYPYYAENRYCLAQKGN-KRVTID 241
           QE+VDCD  ++ GC GG  D AFEF+ +  GIT E NYPY A +  C   K N   V+ID
Sbjct: 183 QELVDCDTDQNQGCNGGLMDYAFEFIKQRGGITTEANYPYEAYDGTCDVSKENAPAVSID 242

Query: 242 GYENVPPNNENALKKAVAHQPVAVSIAASGRRFRYYSEASSFGMFTENDYCGDRIDHTVV 301
           G+ENVP N+ENAL KAVA+QPV+V+I A G  F++YSE    G+FT +  CG  +DH V 
Sbjct: 243 GHENVPENDENALLKAVANQPVSVAIDAGGSDFQFYSE----GVFTGS--CGTELDHGVA 302

Query: 302 VVGYGTEEDGTDYWIIKNSWGTNWGLEGYMKMQRGAQQPEGVCGLAMNPSYPIK 353
           +VGYGT  DGT YW +KNSWG  WG +GY++M+RG    EG+CG+AM  SYPIK
Sbjct: 303 IVGYGTTIDGTKYWTVKNSWGPEWGEKGYIRMERGISDKEGLCGIAMEASYPIK 343

BLAST of Bhi09G000297 vs. ExPASy Swiss-Prot
Match: P12412 (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 1.3e-103
Identity = 190/354 (53.67%), Postives = 249/354 (70.34%), Query Frame = 0

Query: 1   MKFLIVPLVLIVLTFGLCESFKFEGKELESEESLWHLYKRWSSHHRILRNGREMHNRFKV 60
           MK L+  ++ + L  G+  SF F  K+LESEESLW LY+RW SHH + R+  E H RF V
Sbjct: 3   MKKLLWVVLSLSLVLGVANSFDFHEKDLESEESLWDLYERWRSHHTVSRSLGEKHKRFNV 62

Query: 61  FKENAKYVFKVNQMNKSLKLKLNQFADMSDDEFINTHVSSNITYYKNLHAKKIEALGGRV 120
           FK N  +V   N+M+K  KLKLN+FADM++ EF +T+  S + ++K     +  +     
Sbjct: 63  FKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQHGS----- 122

Query: 121 GGFMYEHVEDLPSSIDWRKKGAVTDIKNQGRCGSCWAFAAVAVVEGINQIKTNKLVSLSE 180
           G FMYE V  +P+S+DWRKKGAVTD+K+QG+CGSCWAF+ +  VEGINQIKTNKLVSLSE
Sbjct: 123 GTFMYEKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSE 182

Query: 181 QEVVDCDYKDG-GCGGGFYDSAFEFMMENNGITIEENYPYYAENRYCLAQKGNK-RVTID 240
           QE+VDCD ++  GC GG  +SAFEF+ +  GIT E NYPY A+   C   K N   V+ID
Sbjct: 183 QELVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAVSID 242

Query: 241 GYENVPPNNENALKKAVAHQPVAVSIAASGRRFRYYSEASSFGMFTENDYCGDRIDHTVV 300
           G+ENVP N+ENAL KAVA+QPV+V+I A G  F++YSE    G+FT +  C   ++H V 
Sbjct: 243 GHENVPVNDENALLKAVANQPVSVAIDAGGSDFQFYSE----GVFTGD--CNTDLNHGVA 302

Query: 301 VVGYGTEEDGTDYWIIKNSWGTNWGLEGYMKMQRGAQQPEGVCGLAMNPSYPIK 353
           +VGYGT  DGT+YWI++NSWG  WG +GY++MQR   + EG+CG+AM  SYPIK
Sbjct: 303 IVGYGTTVDGTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPIK 345

BLAST of Bhi09G000297 vs. ExPASy Swiss-Prot
Match: P25803 (Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 375.2 bits (962), Expect = 8.3e-103
Identity = 190/351 (54.13%), Postives = 244/351 (69.52%), Query Frame = 0

Query: 8   LVLIVLTF----GLCESFKFEGKELESEESLWHLYKRWSSHHRILRNGREMHNRFKVFKE 67
           L+ +VL+F    G+  SF F  K+L SEESLW LY+RW SHH + R+  E H RF VFK 
Sbjct: 6   LLWVVLSFSLVLGVANSFDFHDKDLASEESLWDLYERWRSHHTVSRSLGEKHKRFNVFKA 65

Query: 68  NAKYVFKVNQMNKSLKLKLNQFADMSDDEFINTHVSSNITYYKNLHAKKIEALGGRVGGF 127
           N  +V   N+M+K  KLKLN+FADM++ EF +T+  S +      H +         G F
Sbjct: 66  NLMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVN-----HPRMFRGTPHENGAF 125

Query: 128 MYEHVEDLPSSIDWRKKGAVTDIKNQGRCGSCWAFAAVAVVEGINQIKTNKLVSLSEQEV 187
           MYE V  +P S+DWRKKGAVTD+K+QG+CGSCWAF+ V  VEGINQIKTNKLV+LSEQE+
Sbjct: 126 MYEKVVSVPPSVDWRKKGAVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVALSEQEL 185

Query: 188 VDCDYKDG-GCGGGFYDSAFEFMMENNGITIEENYPYYAENRYCLAQKGNK-RVTIDGYE 247
           VDCD ++  GC GG  +SAFEF+ +  GIT E NYPY A+   C A K N   V+IDG+E
Sbjct: 186 VDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAVSIDGHE 245

Query: 248 NVPPNNENALKKAVAHQPVAVSIAASGRRFRYYSEASSFGMFTENDYCGDRIDHTVVVVG 307
           NVP N+E+AL KAVA+QPV+V+I A G  F++YSE    G+FT +  C   ++H V +VG
Sbjct: 246 NVPANDEDALLKAVANQPVSVAIDAGGSDFQFYSE----GVFTGD--CSTDLNHGVAIVG 305

Query: 308 YGTEEDGTDYWIIKNSWGTNWGLEGYMKMQRGAQQPEGVCGLAMNPSYPIK 353
           YGT  DGT+YWI++NSWG  WG  GY++MQR   + EG+CG+AM PSYPIK
Sbjct: 306 YGTTVDGTNYWIVRNSWGPEWGEHGYIRMQRNISKKEGLCGIAMLPSYPIK 345

BLAST of Bhi09G000297 vs. ExPASy Swiss-Prot
Match: Q9FGR9 (KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 PE=1 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 6.8e-97
Identity = 180/354 (50.85%), Postives = 237/354 (66.95%), Query Frame = 0

Query: 1   MKFLIVPLVLIVLTFGLCESFKFEGKELESEESLWHLYKRWSSHHRILRNGREMHNRFKV 60
           MK  IV  + +++     +   F  K++ESE SLW LY+RW SHH + R+  E   RF V
Sbjct: 1   MKRFIVLALCMLMVLETTKGLDFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFNV 60

Query: 61  FKENAKYVFKVNQMNKSLKLKLNQFADMSDDEFINTHVSSNITYYKNLHAKKIEALGGRV 120
           FK N K++ + N+ +KS KLKLN+F DM+ +EF  T+  SNI +++    +K        
Sbjct: 61  FKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEK-----KAT 120

Query: 121 GGFMYEHVEDLPSSIDWRKKGAVTDIKNQGRCGSCWAFAAVAVVEGINQIKTNKLVSLSE 180
             FMY +V  LP+S+DWRK GAVT +KNQG+CGSCWAF+ V  VEGINQI+T KL SLSE
Sbjct: 121 KSFMYANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTSLSE 180

Query: 181 QEVVDCD-YKDGGCGGGFYDSAFEFMMENNGITIEENYPYYAENRYCLAQKGNKR-VTID 240
           QE+VDCD  ++ GC GG  D AFEF+ E  G+T E  YPY A +  C   K N   V+ID
Sbjct: 181 QELVDCDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSID 240

Query: 241 GYENVPPNNENALKKAVAHQPVAVSIAASGRRFRYYSEASSFGMFTENDYCGDRIDHTVV 300
           G+E+VP N+E+ L KAVA+QPV+V+I A G  F++YSE    G+FT    CG  ++H V 
Sbjct: 241 GHEDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSE----GVFTGR--CGTELNHGVA 300

Query: 301 VVGYGTEEDGTDYWIIKNSWGTNWGLEGYMKMQRGAQQPEGVCGLAMNPSYPIK 353
           VVGYGT  DGT YWI+KNSWG  WG +GY++MQRG +  EG+CG+AM  SYP+K
Sbjct: 301 VVGYGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIAMEASYPLK 343

BLAST of Bhi09G000297 vs. ExPASy Swiss-Prot
Match: Q9STL4 (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 PE=1 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 2.9e-95
Identity = 184/353 (52.12%), Postives = 242/353 (68.56%), Query Frame = 0

Query: 2   KFLIVPLVLIVLTFGLCESFKFEGKELESEESLWHLYKRWSSHHRILRNGREMHNRFKVF 61
           K L++ L  +V+    C  F ++ KE+ESEE L  LY RW SHH + R+  E   RF VF
Sbjct: 3   KLLLIFLFSLVILQTAC-GFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFNVF 62

Query: 62  KENAKYVFKVNQMNKSLKLKLNQFADMSDDEFINTHVSSNITYYKNLHAKKIEALGGRVG 121
           + N  +V   N+ N+S KLKLN+FAD++ +EF N +  SNI +++ L   K    G +  
Sbjct: 63  RHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPK---RGSKQF 122

Query: 122 GFMYEHVEDLPSSIDWRKKGAVTDIKNQGRCGSCWAFAAVAVVEGINQIKTNKLVSLSEQ 181
            + +E++  LPSS+DWRKKGAVT+IKNQG+CGSCWAF+ VA VEGIN+IKTNKLVSLSEQ
Sbjct: 123 MYDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQ 182

Query: 182 EVVDCDYKDG-GCGGGFYDSAFEFMMENNGITIEENYPYYAENRYCLAQKGN-KRVTIDG 241
           E+VDCD K   GC GG  + AFEF+ +N GIT E++YPY   +  C A K N   VTIDG
Sbjct: 183 ELVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDG 242

Query: 242 YENVPPNNENALKKAVAHQPVAVSIAASGRRFRYYSEASSFGMFTENDYCGDRIDHTVVV 301
           +E+VP N+ENAL KAVA+QPV+V+I A    F++YSE    G+FT +  CG  ++H V  
Sbjct: 243 HEDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSE----GVFTGS--CGTELNHGVAA 302

Query: 302 VGYGTEEDGTDYWIIKNSWGTNWGLEGYMKMQRGAQQPEGVCGLAMNPSYPIK 353
           VGYG+E  G  YWI++NSWG  WG  GY+K++R   +PEG CG+AM  SYPIK
Sbjct: 303 VGYGSER-GKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of Bhi09G000297 vs. ExPASy TrEMBL
Match: A0A0A0KGB1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G490180 PE=3 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 9.4e-150
Identity = 262/354 (74.01%), Postives = 298/354 (84.18%), Query Frame = 0

Query: 1   MKFLIVPLVLIVLTFGLCESFKFEGKELESEESLWHLYKRWSSHHRILRNGREMHNRFKV 60
           MKFLIVPLVL+  +  +CESF+ E K+ ESE+SL  LYKRWSSHHRI RN  EMHNRFKV
Sbjct: 4   MKFLIVPLVLVAFSCNICESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHNRFKV 63

Query: 61  FKENAKYVFKVNQMNKSLKLKLNQFADMSDDEFINTHVSSNITYYKNLHAKKIEALGGRV 120
           FK NAK+VFKVN M KSLKLKLNQFADMSDDEF N + SSNITYYK+LHAKKIEA GGR+
Sbjct: 64  FKNNAKHVFKVNLMGKSLKLKLNQFADMSDDEFRNMY-SSNITYYKDLHAKKIEATGGRI 123

Query: 121 GGFMYEHVEDLPSSIDWRKKGAVTDIKNQGRCGSCWAFAAVAVVEGINQIKTNKLVSLSE 180
           GGFMYEH  ++PSSIDWRKKGAV  IKNQGRCGSCWAFAAVA VE I+QIKTN+LVSLSE
Sbjct: 124 GGFMYEHANNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSE 183

Query: 181 QEVVDCDYKDGGCGGGFYDSAFEFMMENNGITIEENYPYYAENRYCLAQKG-NKRVTIDG 240
           +EV+DCDY+DGGC GGFY+SAFEFMM+N+G+TIE+NYPYY  N YC  + G NKRV IDG
Sbjct: 184 EEVLDCDYRDGGCRGGFYNSAFEFMMDNDGVTIEDNYPYYEGNGYCRRRGGRNKRVRIDG 243

Query: 241 YENVPPNNENALKKAVAHQPVAVSIAASGRRFRYYSEASSFGMFTENDYCGDRIDHTVVV 300
           YENVP NNE AL KAVAHQPVAV+IA+ G  F++Y      GMFTEND+CG  IDHTVVV
Sbjct: 244 YENVPRNNEYALMKAVAHQPVAVAIASGGSDFKFYGG----GMFTENDFCGFNIDHTVVV 303

Query: 301 VGYGTEEDGTDYWIIKNSWGTNWGLEGYMKMQRGAQQPEGVCGLAMNPSYPIKY 354
           VGYGT+EDG DYWII+N +G  WG+ GYMKMQRGA  P+GVCG+AM P+YP+KY
Sbjct: 304 VGYGTDEDG-DYWIIRNQYGHRWGMNGYMKMQRGAHSPQGVCGMAMQPAYPVKY 351

BLAST of Bhi09G000297 vs. ExPASy TrEMBL
Match: A0A1S3BYU0 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494878 PE=3 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 7.5e-147
Identity = 259/354 (73.16%), Postives = 294/354 (83.05%), Query Frame = 0

Query: 1   MKFLIVPLVLIVLTFGLCESFKFEGKELESEESLWHLYKRWSSHHRILRNGREMHNRFKV 60
           MKFLIVPLVLI  TF LCESF+ E K+ ESE+SL  LYKRWSSHHRI RN  EMH RFKV
Sbjct: 4   MKFLIVPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRFKV 63

Query: 61  FKENAKYVFKVNQMNKSLKLKLNQFADMSDDEFINTHVSSNITYYKNLHAKKIEALGGRV 120
           FK+NAKYVFK N M +SLKL+LNQFADMSDDEF + H  SNITYYKNLHAK      GRV
Sbjct: 64  FKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIH-GSNITYYKNLHAK-----NGRV 123

Query: 121 GGFMYEHVEDLPSSIDWRKKGAVTDIKNQGRCGSCWAFAAVAVVEGINQIKTNKLVSLSE 180
           GGFMYEH  D+PSSIDWRKKGAV  IKNQGRCGSCWAFAAVA VE I+QIKTN+LVSLSE
Sbjct: 124 GGFMYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSE 183

Query: 181 QEVVDCDYKDGGCGGGFYDSAFEFMMENNGITIEENYPYYAENRYCLAQKG-NKRVTIDG 240
           QEVVDCDY+D GC GGFY+SAFEFMMEN GIT+E+NYPYY  + YC  + G N+RVTIDG
Sbjct: 184 QEVVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTIDG 243

Query: 241 YENVPPNNENALKKAVAHQPVAVSIAASGRRFRYYSEASSFGMFTENDYCGDRIDHTVVV 300
           YENVP NNE+AL KAVAHQPVAV+IA+SG  FR+Y +    GMFTE D+CG  IDHTVVV
Sbjct: 244 YENVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQ----GMFTEQDFCGYNIDHTVVV 303

Query: 301 VGYGTEEDGTDYWIIKNSWGTNWGLEGYMKMQRGAQQPEGVCGLAMNPSYPIKY 354
           VGYGT+E+  DYWII+N +GT WG+ GYMKMQRGA+ P+GVCG+A+ P+YP+K+
Sbjct: 304 VGYGTDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAIQPAYPVKH 347

BLAST of Bhi09G000297 vs. ExPASy TrEMBL
Match: A0A5D3D043 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001320 PE=3 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 9.1e-145
Identity = 257/354 (72.60%), Postives = 292/354 (82.49%), Query Frame = 0

Query: 1   MKFLIVPLVLIVLTFGLCESFKFEGKELESEESLWHLYKRWSSHHRILRNGREMHNRFKV 60
           MKFLIVPLVLI  TF LCESF+ E K+ ESE+SL  LYKRWSSHHRI RN  EMH RFKV
Sbjct: 4   MKFLIVPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRFKV 63

Query: 61  FKENAKYVFKVNQMNKSLKLKLNQFADMSDDEFINTHVSSNITYYKNLHAKKIEALGGRV 120
           FK+NAKYVFK N M +SLKL+LNQFADMSDDEF + H  SNITYYKNLHAK      GRV
Sbjct: 64  FKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIH-GSNITYYKNLHAK-----NGRV 123

Query: 121 GGFMYEHVEDLPSSIDWRKKGAVTDIKNQGRCGSCWAFAAVAVVEGINQIKTNKLVSLSE 180
           GGFMYEH  D+PSSIDWRKKGAV  IKNQGRCGSCWAFAAVA VE I+QIKTN+LVSLSE
Sbjct: 124 GGFMYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSE 183

Query: 181 QEVVDCDYKDGGCGGGFYDSAFEFMMENNGITIEENYPYYAENRYCLAQKG-NKRVTIDG 240
           QEVVDCDY+D GC GGFY+SAFEFMMEN GIT+E+NYPYY  + YC  + G N+RVTIDG
Sbjct: 184 QEVVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTIDG 243

Query: 241 YENVPPNNENALKKAVAHQPVAVSIAASGRRFRYYSEASSFGMFTENDYCGDRIDHTVVV 300
           YENVP NNE+AL KAVAHQPVAV+IA+SGR   + +     GMFTE D+CG  IDHTVVV
Sbjct: 244 YENVPRNNEHALMKAVAHQPVAVAIASSGRILNFDNYLQ--GMFTEQDFCGYNIDHTVVV 303

Query: 301 VGYGTEEDGTDYWIIKNSWGTNWGLEGYMKMQRGAQQPEGVCGLAMNPSYPIKY 354
           VGYGT+E+  DYWII+N +GT WG+ GYMKMQRGA+ P+GVCG+A+ P+YP+K+
Sbjct: 304 VGYGTDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAIQPAYPVKH 349

BLAST of Bhi09G000297 vs. ExPASy TrEMBL
Match: A0A1S3BYQ3 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494879 PE=3 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 5.9e-144
Identity = 255/354 (72.03%), Postives = 292/354 (82.49%), Query Frame = 0

Query: 1   MKFLIVPLVLIVLTFGLCESFKFEGKELESEESLWHLYKRWSSHHRILRNGREMHNRFKV 60
           MKFLIVPLVLI LT  LCESF+ E K+ ESE+SL  LYKRWSSHHRI RN  EMH RFKV
Sbjct: 5   MKFLIVPLVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRFKV 64

Query: 61  FKENAKYVFKVNQMNKSLKLKLNQFADMSDDEFINTHVSSNITYYKNLHAKKIEALGGRV 120
           FK+NAK+VFK N M +SLKL+LNQFADMSDDEF + H  SNITYYKNLHAK      G V
Sbjct: 65  FKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIH-GSNITYYKNLHAKT-----GHV 124

Query: 121 GGFMYEHVEDLPSSIDWRKKGAVTDIKNQGRCGSCWAFAAVAVVEGINQIKTNKLVSLSE 180
           GGFMYEH +++PSSIDWRKKGAV  IKNQG CGSCWAFAAVA VE I+QIKTN+LVSLSE
Sbjct: 125 GGFMYEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSE 184

Query: 181 QEVVDCDYKDGGCGGGFYDSAFEFMMENNGITIEENYPYYAENRYCLAQKG-NKRVTIDG 240
           QEVVDCDY+DGGC GG Y+SAFEFMMEN GIT+E+NYPYY  + YC  + G N+RV IDG
Sbjct: 185 QEVVDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDG 244

Query: 241 YENVPPNNENALKKAVAHQPVAVSIAASGRRFRYYSEASSFGMFTENDYCGDRIDHTVVV 300
           YENVP NNE+AL KAVAHQPVAV+IA+SG  FR+Y +    GMFTE D+CG  IDHTVVV
Sbjct: 245 YENVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQ----GMFTEQDFCGYNIDHTVVV 304

Query: 301 VGYGTEEDGTDYWIIKNSWGTNWGLEGYMKMQRGAQQPEGVCGLAMNPSYPIKY 354
           VGYG++E+  DYWII+N +GT WG+ GYMKMQRGA+ P+GVCG+AM P+YP+KY
Sbjct: 305 VGYGSDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVKY 348

BLAST of Bhi09G000297 vs. ExPASy TrEMBL
Match: A0A5A7TM64 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003430 PE=3 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 1.7e-143
Identity = 254/354 (71.75%), Postives = 291/354 (82.20%), Query Frame = 0

Query: 1   MKFLIVPLVLIVLTFGLCESFKFEGKELESEESLWHLYKRWSSHHRILRNGREMHNRFKV 60
           MKFLIVP VLI LT  LCESF+ E K+ ESE+SL  LYKRWSSHHRI RN  EMH RFKV
Sbjct: 5   MKFLIVPFVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRFKV 64

Query: 61  FKENAKYVFKVNQMNKSLKLKLNQFADMSDDEFINTHVSSNITYYKNLHAKKIEALGGRV 120
           FK+NAK+VFK N M +SLKL+LNQFADMSDDEF + H  SNITYYKNLHAK      G V
Sbjct: 65  FKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIH-GSNITYYKNLHAKT-----GHV 124

Query: 121 GGFMYEHVEDLPSSIDWRKKGAVTDIKNQGRCGSCWAFAAVAVVEGINQIKTNKLVSLSE 180
           GGFMYEH +++PSSIDWRKKGAV  IKNQG CGSCWAFAAVA VE I+QIKTN+LVSLSE
Sbjct: 125 GGFMYEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSE 184

Query: 181 QEVVDCDYKDGGCGGGFYDSAFEFMMENNGITIEENYPYYAENRYCLAQKG-NKRVTIDG 240
           QEVVDCDY+DGGC GG Y+SAFEFMMEN GIT+E+NYPYY  + YC  + G N+RV IDG
Sbjct: 185 QEVVDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDG 244

Query: 241 YENVPPNNENALKKAVAHQPVAVSIAASGRRFRYYSEASSFGMFTENDYCGDRIDHTVVV 300
           YENVP NNE+AL KAVAHQPVAV+IA+SG  FR+Y +    GMFTE D+CG  IDHTVVV
Sbjct: 245 YENVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQ----GMFTEQDFCGYNIDHTVVV 304

Query: 301 VGYGTEEDGTDYWIIKNSWGTNWGLEGYMKMQRGAQQPEGVCGLAMNPSYPIKY 354
           VGYG++E+  DYWII+N +GT WG+ GYMKMQRGA+ P+GVCG+AM P+YP+KY
Sbjct: 305 VGYGSDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVKY 348

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G50260.14.8e-9850.85Cysteine proteinases superfamily protein [more]
AT3G48340.12.0e-9652.12Cysteine proteinases superfamily protein [more]
AT3G48350.17.7e-9648.73Cysteine proteinases superfamily protein [more]
AT3G19390.11.0e-7945.53Granulin repeat cysteine protease family protein [more]
AT5G45890.11.5e-7845.27senescence-associated gene 12 [more]
Match NameE-valueIdentityDescription
O650399.8e-10455.08Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1[more]
P124121.3e-10353.67Vignain OS=Vigna mungo OX=3915 PE=1 SV=1[more]
P258038.3e-10354.13Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2[more]
Q9FGR96.8e-9750.85KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 ... [more]
Q9STL42.9e-9552.12KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KGB19.4e-15074.01Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G490180 PE=3 SV=1[more]
A0A1S3BYU07.5e-14773.16ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494878 PE=3 SV=1[more]
A0A5D3D0439.1e-14572.60Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001... [more]
A0A1S3BYQ35.9e-14472.03ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494879 PE=3 SV=1[more]
A0A5A7TM641.7e-14371.75Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G0034... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 149..164
score: 59.25
coord: 311..317
score: 75.14
coord: 295..305
score: 54.69
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 131..351
e-value: 4.6E-102
score: 355.1
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 131..351
e-value: 2.0E-72
score: 243.9
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 38..93
e-value: 7.3E-9
score: 45.5
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 38..93
e-value: 4.2E-10
score: 39.9
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 14..353
e-value: 1.5E-111
score: 375.3
NoneNo IPR availablePANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 18..352
NoneNo IPR availablePANTHERPTHR12411:SF706KDEL-TAILED CYSTEINE ENDOPEPTIDASE CEP1coord: 18..352
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 149..160
IPR025661Cysteine peptidase, asparagine active sitePROSITEPS00640THIOL_PROTEASE_ASNcoord: 311..330
IPR039417Papain-like cysteine endopeptidaseCDDcd02248Peptidase_C1Acoord: 132..350
e-value: 6.42653E-102
score: 297.228
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 31..351

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi09M000297Bhi09M000297mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0005615 extracellular space
cellular_component GO:0005764 lysosome
cellular_component GO:0005886 plasma membrane
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0008234 cysteine-type peptidase activity