Bhi09G000090 (gene) Wax gourd (B227) v1

Overview


Name	Bhi09G000090
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Acyl-[acyl-carrier-protein] hydrolase
Location	chr9: 2249894 .. 2250843 (-)
RNA-Seq Expression	Bhi09G000090
Synteny	Bhi09G000090

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACTCAATTTTGAAGAAAGAACAAAGATTTGGAGATATAAATAGAGGTACTTTGCCATTCAAAGGTCATCCAAAAATTTGTTTAGAAAGGCTCAAAACACCTAACATTATTATTGCTAAGTCCACCATTGCAAGTAAGATAAAAAATTTATTTCTTTCTCCAACATTTTTGTGTATGTTTTTTAAAGAGAGAATAATTCGTTGATTGTGATTGATTTTTTCCCCTTTTACAGGTGAGGTTAGAAAGATAGATAGATATGAGCCTAGAAATCTTGACATAACTCATGGAAACTTAACAATGGGAGAGTTATTGAACGATGGTTTGGTTTACCAACGTCATTTCTTGGTCAAATCGTACGAGTCGGGGCCTGATAAAAATATGACAATGTTTTTCATCATCAACCAGTTACAGGTTCGAGTTTGATTTTACATTTCTAATCGCATTTTTCATATTGATAAAAGTGATTTTGGATGATTTGAAGTAGTTTTTGCAATGATTTTAAAAGTCATAAAAGTGATCCGTGATCATCTGTTATTATTATTATTTTTTTAATTTGTAACTTTTTTTTTTTGATTAGGAGTCAAAACTAAACCACTTGAAAAGTTGTGGATCGGTGATGACAGGATTGGGCACAACACCGGAAATGACTAAAAGGGGCCTCCTATGGGTGGTTCATAAATTGCAGGTGGTGGTGGACCAATATCCTTCTTGGTAATTTAATTATTTTTTCCCCTTAAAATTTGCATAATTTAACAAGTATTTATTTTATTAAAAAAAAAATTGTGGTATTTTTGAATTATATATCTATGTTTTTGTTTTTGTTTTTAAGGAATGATGTTGTTCAATTACGAACATGGAGTTGTGTTCATAGAAAAAATAGTTATCGTCGAGATTGGATTGTTCGTGACTATAAAACTGGTCAAGTCTTGATTCGTGCTACTTGGTAA

mRNA sequence

ATGAACTCAATTTTGAAGAAAGAACAAAGATTTGGAGATATAAATAGAGGTACTTTGCCATTCAAAGGTCATCCAAAAATTTGTTTAGAAAGGCTCAAAACACCTAACATTATTATTGCTAAGTCCACCATTGCAAGTGAGGAGTCAAAACTAAACCACTTGAAAAGTTGTGGATCGGTGGTGGTGGACCAATATCCTTCTTGGAATGATGTTGTTCAATTACGAACATGGAGTTGTGTTCATAGAAAAAATAGTTATCGTCGAGATTGGATTGTTCGTGACTATAAAACTGGTCAAGTCTTGATTCGTGCTACTTGGTAA

Coding sequence (CDS)

ATGAACTCAATTTTGAAGAAAGAACAAAGATTTGGAGATATAAATAGAGGTACTTTGCCATTCAAAGGTCATCCAAAAATTTGTTTAGAAAGGCTCAAAACACCTAACATTATTATTGCTAAGTCCACCATTGCAAGTGAGGAGTCAAAACTAAACCACTTGAAAAGTTGTGGATCGGTGGTGGTGGACCAATATCCTTCTTGGAATGATGTTGTTCAATTACGAACATGGAGTTGTGTTCATAGAAAAAATAGTTATCGTCGAGATTGGATTGTTCGTGACTATAAAACTGGTCAAGTCTTGATTCGTGCTACTTGGTAA

Protein sequence

MNSILKKEQRFGDINRGTLPFKGHPKICLERLKTPNIIIAKSTIASEESKLNHLKSCGSVVVDQYPSWNDVVQLRTWSCVHRKNSYRRDWIVRDYKTGQVLIRATW

Homology

[Close]

BLAST of Bhi09G000090 vs. TAIR 10
Match: AT1G08510.1 (fatty acyl-ACP thioesterases B )

HSP 1 Score: 63.9 bits (154), Expect = 8.7e-11
Identity = 32/83 (38.55%), Postives = 43/83 (51.81%), Query Frame = 0


Query: 47  EESKLNHLKSCG------------------------SVVVDQYPSWNDVVQLRTWSCVHR 106
           +E+ LNH+K+ G                         VVVD+YP+W DVV++ TW     
Sbjct: 166 QETALNHVKTAGLLGDGFGSTPEMFKKNLIWVVTRMQVVVDKYPTWGDVVEVDTWVSQSG 225

[Close]

BLAST of Bhi09G000090 vs. TAIR 10
Match: AT4G13050.1 (Acyl-ACP thioesterase )

HSP 1 Score: 52.0 bits (123), Expect = 3.4e-07
Identity = 20/46 (43.48%), Postives = 32/46 (69.57%), Query Frame = 0


Query: 60  VVVDQYPSWNDVVQLRTWSCVHRKNSYRRDWIVRDYKTGQVLIRAT 106
           + + +YP+W+DVV++ TW     +   RRDWI++D  TG+V+ RAT
Sbjct: 147 IEIYKYPAWSDVVEIETWCQSEGRIGTRRDWILKDCATGEVIGRAT 192

[Close]

BLAST of Bhi09G000090 vs. TAIR 10
Match: AT3G25110.1 (fatA acyl-ACP thioesterase )

HSP 1 Score: 50.1 bits (118), Expect = 1.3e-06
Identity = 20/46 (43.48%), Postives = 30/46 (65.22%), Query Frame = 0


Query: 60  VVVDQYPSWNDVVQLRTWSCVHRKNSYRRDWIVRDYKTGQVLIRAT 106
           + + +YP+W DVV++ TW     +   RRDWI++D  TG+V  RAT
Sbjct: 148 IEIYKYPAWGDVVEIETWCQSEGRIGTRRDWILKDSVTGEVTGRAT 193

[Close]

BLAST of Bhi09G000090 vs. ExPASy Swiss-Prot
Match: Q9SJE2 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FATB PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.2e-09
Identity = 32/83 (38.55%), Postives = 43/83 (51.81%), Query Frame = 0


Query: 47  EESKLNHLKSCG------------------------SVVVDQYPSWNDVVQLRTWSCVHR 106
           +E+ LNH+K+ G                         VVVD+YP+W DVV++ TW     
Sbjct: 166 QETALNHVKTAGLLGDGFGSTPEMFKKNLIWVVTRMQVVVDKYPTWGDVVEVDTWVSQSG 225

[Close]

BLAST of Bhi09G000090 vs. ExPASy Swiss-Prot
Match: Q9SQI3 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Gossypium hirsutum OX=3635 GN=FATB1 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.6e-09
Identity = 26/46 (56.52%), Postives = 34/46 (73.91%), Query Frame = 0


Query: 60  VVVDQYPSWNDVVQLRTWSCVHRKNSYRRDWIVRDYKTGQVLIRAT 106
           VVVD+YP+W DVVQ+ TW     KN  RRDW+V + +TG++L RAT
Sbjct: 198 VVVDRYPTWGDVVQVDTWVSASGKNGMRRDWLVSNSETGEILTRAT 243

[Close]

BLAST of Bhi09G000090 vs. ExPASy Swiss-Prot
Match: G3ESV0 (Acyl-[acyl-carrier-protein] hydrolase FATB2, chloroplastic (Fragment) OS=Cuphea viscosissima OX=857185 GN=FATB2 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 4.0e-08
Identity = 27/83 (32.53%), Postives = 42/83 (50.60%), Query Frame = 0


Query: 47  EESKLNHLKSCG------------------------SVVVDQYPSWNDVVQLRTWSCVHR 106
           +E+ LNH+K+ G                         V+V++YP+W D V++ TW     
Sbjct: 162 QETALNHVKTAGLSNDGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSG 221

[Close]

BLAST of Bhi09G000090 vs. ExPASy Swiss-Prot
Match: Q39513 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Cuphea hookeriana OX=36775 GN=FATB1 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 8.8e-08
Identity = 21/46 (45.65%), Postives = 32/46 (69.57%), Query Frame = 0


Query: 60  VVVDQYPSWNDVVQLRTWSCVHRKNSYRRDWIVRDYKTGQVLIRAT 106
           V+V++YP+W D V++ TW     KN  RRDW++ D  TG++L RA+
Sbjct: 202 VMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRAS 247

[Close]

BLAST of Bhi09G000090 vs. ExPASy Swiss-Prot
Match: G3ESV1 (Acyl-[acyl-carrier-protein] hydrolase FATB3, chloroplastic OS=Cuphea viscosissima OX=857185 GN=FATB3 PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 3.4e-07
Identity = 26/83 (31.33%), Postives = 40/83 (48.19%), Query Frame = 0


Query: 47  EESKLNHLKSCG------------------------SVVVDQYPSWNDVVQLRTWSCVHR 106
           +E+ LNH KS G                         + V++YP+W D +++ TW     
Sbjct: 162 QETSLNHCKSLGLLNDGFGRTPEMCKRDLIWVVTKMQIEVNRYPTWGDTIEVTTWVSESG 221

[Close]

BLAST of Bhi09G000090 vs. ExPASy TrEMBL
Match: A0A5A7UZ85 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G002510 PE=3 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 2.3e-27
Identity = 82/186 (44.09%), Postives = 90/186 (48.39%), Query Frame = 0


Query: 2   NSILKKEQRFGDINRGTLPFKGHPKICLERLKTPNIIIAKSTIASE-------------- 61
           NS LKKEQRFGD N G L  K HPK+ LE L+T N IIAKS+IASE              
Sbjct: 17  NSNLKKEQRFGDSNTGNLQLKNHPKLFLEGLRTSN-IIAKSSIASEVRKINSYESRNLDI 76

Query: 62  --------------------------------------------ESKLNHLKSCG----- 106
                                                       ESKLNHLKSCG     
Sbjct: 77  THGNLTMGELLNDGLVYQRHFLVKSYESGPDKKMTMFFIINQLHESKLNHLKSCGLVTTG 136

[Close]

BLAST of Bhi09G000090 vs. ExPASy TrEMBL
Match: A0A6J1H8Q2 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461080 PE=3 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 8.5e-14
Identity = 44/87 (50.57%), Postives = 50/87 (57.47%), Query Frame = 0


Query: 47  EESKLNHLKSCG-----------------------------SVVVDQYPSWNDVVQLRTW 105
           +ESKLNH  SCG                              VVVDQ+PSWNDVV+LRTW
Sbjct: 104 QESKLNHFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTW 163

[Close]

BLAST of Bhi09G000090 vs. ExPASy TrEMBL
Match: A0A6J1KZF4 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111497736 PE=3 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.1e-13
Identity = 44/87 (50.57%), Postives = 50/87 (57.47%), Query Frame = 0


Query: 47  EESKLNHLKSCG-----------------------------SVVVDQYPSWNDVVQLRTW 105
           +ESKLNH  SCG                              VVVDQ+PSWNDVV+LRTW
Sbjct: 104 QESKLNHFISCGLVKTGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTW 163

[Close]

BLAST of Bhi09G000090 vs. ExPASy TrEMBL
Match: A0A6J1CMR0 (Acyl-[acyl-carrier-protein] hydrolase OS=Momordica charantia OX=3673 GN=LOC111012889 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 4.2e-13
Identity = 41/82 (50.00%), Postives = 48/82 (58.54%), Query Frame = 0


Query: 47  EESKLNHLKSCG------------------------SVVVDQYPSWNDVVQLRTWSCVHR 105
           +ES +NH KSCG                         VVVD YPSWND+VQL TW CV  
Sbjct: 9   QESAINHFKSCGLVETGYASTREMDKRGLIWVVHKLQVVVDLYPSWNDIVQLSTWVCVCG 68

[Close]

BLAST of Bhi09G000090 vs. ExPASy TrEMBL
Match: A0A6P3Z5F8 (Acyl-[acyl-carrier-protein] hydrolase OS=Ziziphus jujuba OX=326968 GN=LOC107406718 PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 8.8e-11
Identity = 35/82 (42.68%), Postives = 45/82 (54.88%), Query Frame = 0


Query: 47  EESKLNHLKSCG------------------------SVVVDQYPSWNDVVQLRTWSCVHR 105
           +ES LNH+K  G                         +VVD YPSW DV+Q+ TW+C   
Sbjct: 129 QESALNHVKGAGILAEGFGSTPEMSRRNLIWVVYRMQIVVDSYPSWGDVIQVNTWTCASG 188

The following BLAST results are available for this feature:

BLAST of Bhi09G000090 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G08510.1	8.7e-11	38.55	fatty acyl-ACP thioesterases B	[more]
AT4G13050.1	3.4e-07	43.48	Acyl-ACP thioesterase	[more]
AT3G25110.1	1.3e-06	43.48	fatA acyl-ACP thioesterase	[more]

BLAST of Bhi09G000090 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SJE2	1.2e-09	38.55	Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Arabidopsis thalia...	[more]
Q9SQI3	1.6e-09	56.52	Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Gossypium hirsutum...	[more]
G3ESV0	4.0e-08	32.53	Acyl-[acyl-carrier-protein] hydrolase FATB2, chloroplastic (Fragment) OS=Cuphea ...	[more]
Q39513	8.8e-08	45.65	Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Cuphea hookeriana ...	[more]
G3ESV1	3.4e-07	31.33	Acyl-[acyl-carrier-protein] hydrolase FATB3, chloroplastic OS=Cuphea viscosissim...	[more]

BLAST of Bhi09G000090 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UZ85	2.3e-27	44.09	Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A6J1H8Q2	8.5e-14	50.57	Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461...	[more]
A0A6J1KZF4	1.1e-13	50.57	Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita maxima OX=3661 GN=LOC11149773...	[more]
A0A6J1CMR0	4.2e-13	50.00	Acyl-[acyl-carrier-protein] hydrolase OS=Momordica charantia OX=3673 GN=LOC11101...	[more]
A0A6P3Z5F8	8.8e-11	42.68	Acyl-[acyl-carrier-protein] hydrolase OS=Ziziphus jujuba OX=326968 GN=LOC1074067...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002864	Acyl-ACP thioesterase	PFAM	PF01643	Acyl-ACP_TE	coord: 59..105 e-value: 1.6E-8 score: 34.1
None	No IPR available	GENE3D	3.10.129.10	Hotdog Thioesterase	coord: 49..106 e-value: 2.3E-11 score: 45.3
IPR045023	Acyl-[acyl-carrier-protein] hydrolase FATA/B	PANTHER	PTHR31727	OLEOYL-ACYL CARRIER PROTEIN THIOESTERASE 1, CHLOROPLASTIC	coord: 52..105
IPR029069	HotDog domain superfamily	SUPERFAMILY	54637	Thioesterase/thiol ester dehydrase-isomerase	coord: 60..105

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi09M000090	Bhi09M000090	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006633	fatty acid biosynthetic process
molecular_function	GO:0016297	acyl-[acyl-carrier-protein] hydrolase activity
molecular_function	GO:0016790	thiolester hydrolase activity