Bhi08G001697 (gene) Wax gourd (B227) v1

Overview
NameBhi08G001697
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionGibberellin 2-beta-dioxygenase-like
Locationchr8: 59944597 .. 59946948 (-)
RNA-Seq ExpressionBhi08G001697
SyntenyBhi08G001697
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGATGGTATAAATACCCAACGGAAGCAAAGAGTAATGCCTCCTCCTCCTCATCCATTTCATCATTCAAATTCAAACCATTTCTTCTTCTTCTTCTTCTGTTGTTTAAATCCCTCTTCTTCTTCAATCATGGTGGTTCAATCCCAACTTGTGTTCGACCATTTTGCTCCCATCAAATCCTGCAAACGCACTGCTCTGTTCACCGGAATTCCGGTGATCGATCTTAAAGACCCAGAAGCCAAAACCCACATCGTCAAAGCCTGTGAGGATTTCGGTTTCTTCAAGGTCGCCAACCATGGCGTCTCCGACGACCTCATCGGCGCTCTCGAAGCCCAAGCTCTGAAATTCTTCCATCTCCCTCAATCGGAGAAGGAAAAAGCAGGGCCACCCGACCCCTTTGGATATGGCAGCAAGAGAATTGGCCCAAACGGCGATGTGGGTTGGATTGAATATGTTCTCCTCAATACCAATCCTCAACTCATTTCCGAAAAATCCTTCTCCATTTTCCGGGAAAATCCCGAGATTTTCCGGTAAGATCTTGAAGAAACAGATTACTCTGTTTTTTGCTCTGTGTTTTTGAAACAGAGAGGATATTGATTTGTTGATATTTTTGTAGGTGTTTGGTGGAGGAATATATATTGGCTGTGAAGGAGATGGCTTGTGAAGTGCTGGAAGTTATGGCAGAAGGGCTGAGGATTGAGAAAAGTGCTTTGAGCAGGCTGTTGAAAGATGAAAGGAGTGATTGTTGTTTCAGGCTAAACCATTATCCGCCATGCCCGGAGCTTGAAGCTTTGAGTGGCAGGAATTTGATAGGGTTTGGGGAACATACAGACCCACAAATCATATCTGTCTTGAGATCAAATAACACTACTGGACTTCATATCTGTTTGAGAGATGGAAATTGGGTTTCAGTTCCTCCTGATCACACTTCCTTTTTCATCAATGTGGGTGATTCATTACAGGTAGAATCCCTTCTCTCTCTCTCATTGGAGATCTCTGTTCGTAGGTTACTGTTATTAAAATCGACCATCAAACATAAATAGAAAACGTAAATGGATAGTTAGAAAAAATTGTGAGAACTGCTAGTAGAGTTTTAGAGTTTATATAGTGTATTTATATGAATTTTAGGATCAACATCGTAAATAACATATAAAAGACAAAATTCATAAGAGATTCAATGCATTCCAACATATTACTTTTCATTCATCTTTTCTTTCTCTAGCTACCATTTTCTTGGAAACTTCTCCTTCTTTCTTGCTATCTAGAATGTGTGGAGAAGTCAAAGATGGATAGACAGAGGATCACTTTCTCTATCCAAAAGGCTTTTTTTTTTTTTCCTTTTTCTAAATGGGGAAGGGGAAATGGTGATTTTAATGGGTGGTTTTTAATGTATGTTTGTGTTATTTTGTTGCAGGTAATGACTAATGGGAGGTTTAAGAGTGTAAAGCACAAGGTTTTAGCTGATCCAATAAAATCAAGGTTTTCAATGATCTACTTTGGTGGACCACCTTTGAATGAAAAGATTGCACCTTTACCATCACTATTGGAAGATGGAGAGCAAAGTTTGTACAGAGAATTCACTTGGTGGGATTACAAAAACTCTGCTTATAAATCAAGACTTGCTGATTGTAGGTTAGGCCCTTTTGAGATAAAAAACCTCACCCCTTTGTTGACCTGAAAATCCCCCACAAGAAAAATACTTCCTTTTCAAAATTATGTCACAATGCCAAAGAAATAGAAACAAAAAAGAGAGAGAGGGTCCTTTTGGGATACTTTGAAAATATGTGAAATTCTTGTGTCATTTCCCTATTGTTCTTCTATGTGCTTATGGTTTGGAGAGAGCAAAAGACAGAGAGAGAGAGGGAAAAAAGGTGTATAATTCATAATCACATGATAAAATAATGGATAAATAATAGGGGAATATTTGGCTTAAAAAAGGAAAAAAAAAATGTTTAAATTAGATCATATGTGTAAGGTAGAGATAGAGATGGGAGGGATTTGGCAAGGCTAGGAAACAAATAGAATAGGTTTGTTTTTGTTTCTCACAATGTTCTTTTATTTTGTTAAATATTTTTGCTTTTGGGTTTAAATGTATGAGTAAGGACAACCACTTTCCACCCTCCAATCCCATTAGTACATTTGTCATCACCATCATCATCATCATGTCACCATGTGCAAATGACACCCTCATGTATTTTTCCCCAAAATACCCCTCACTTGCAAGTATACTTAGTCACATAACTCCCCCCTTACCATCTTGTACCAAAGCTTGTGGATTCTAGTGTTATATATGCTTCTCACAATTTCCCATATCTCAAAATTTTAACTTTCTTCTTAGAATGTAAAAAAATCAG

mRNA sequence

CGATGGTATAAATACCCAACGGAAGCAAAGAGTAATGCCTCCTCCTCCTCATCCATTTCATCATTCAAATTCAAACCATTTCTTCTTCTTCTTCTTCTGTTGTTTAAATCCCTCTTCTTCTTCAATCATGGTGGTTCAATCCCAACTTGTGTTCGACCATTTTGCTCCCATCAAATCCTGCAAACGCACTGCTCTGTTCACCGGAATTCCGGTGATCGATCTTAAAGACCCAGAAGCCAAAACCCACATCGTCAAAGCCTGTGAGGATTTCGGTTTCTTCAAGGTCGCCAACCATGGCGTCTCCGACGACCTCATCGGCGCTCTCGAAGCCCAAGCTCTGAAATTCTTCCATCTCCCTCAATCGGAGAAGGAAAAAGCAGGGCCACCCGACCCCTTTGGATATGGCAGCAAGAGAATTGGCCCAAACGGCGATGTGGGTTGGATTGAATATGTTCTCCTCAATACCAATCCTCAACTCATTTCCGAAAAATCCTTCTCCATTTTCCGGGAAAATCCCGAGATTTTCCGGTGTTTGGTGGAGGAATATATATTGGCTGTGAAGGAGATGGCTTGTGAAGTGCTGGAAGTTATGGCAGAAGGGCTGAGGATTGAGAAAAGTGCTTTGAGCAGGCTGTTGAAAGATGAAAGGAGTGATTGTTGTTTCAGGCTAAACCATTATCCGCCATGCCCGGAGCTTGAAGCTTTGAGTGGCAGGAATTTGATAGGGTTTGGGGAACATACAGACCCACAAATCATATCTGTCTTGAGATCAAATAACACTACTGGACTTCATATCTGTTTGAGAGATGGAAATTGGGTTTCAGTTCCTCCTGATCACACTTCCTTTTTCATCAATGTGGGTGATTCATTACAGGTAATGACTAATGGGAGGTTTAAGAGTGTAAAGCACAAGGTTTTAGCTGATCCAATAAAATCAAGGTTTTCAATGATCTACTTTGGTGGACCACCTTTGAATGAAAAGATTGCACCTTTACCATCACTATTGGAAGATGGAGAGCAAAGTTTGTACAGAGAATTCACTTGGTGGGATTACAAAAACTCTGCTTATAAATCAAGACTTGCTGATTGTAGGTTAGGCCCTTTTGAGATAAAAAACCTCACCCCTTTGTTGACCTGAAAATCCCCCACAAGAAAAATACTTCCTTTTCAAAATTATGTCACAATGCCAAAGAAATAGAAACAAAAAAGAGAGAGAGGGTCCTTTTGGGATACTTTGAAAATATGTGAAATTCTTGTGTCATTTCCCTATTGTTCTTCTATGTGCTTATGGTTTGGAGAGAGCAAAAGACAGAGAGAGAGAGGGAAAAAAGGTGTATAATTCATAATCACATGATAAAATAATGGATAAATAATAGGGGAATATTTGGCTTAAAAAAGGAAAAAAAAAATGTTTAAATTAGATCATATGTGTAAGGTAGAGATAGAGATGGGAGGGATTTGGCAAGGCTAGGAAACAAATAGAATAGGTTTGTTTTTGTTTCTCACAATGTTCTTTTATTTTGTTAAATATTTTTGCTTTTGGGTTTAAATGTATGAGTAAGGACAACCACTTTCCACCCTCCAATCCCATTAGTACATTTGTCATCACCATCATCATCATCATGTCACCATGTGCAAATGACACCCTCATGTATTTTTCCCCAAAATACCCCTCACTTGCAAGTATACTTAGTCACATAACTCCCCCCTTACCATCTTGTACCAAAGCTTGTGGATTCTAGTGTTATATATGCTTCTCACAATTTCCCATATCTCAAAATTTTAACTTTCTTCTTAGAATGTAAAAAAATCAG

Coding sequence (CDS)

ATGCCTCCTCCTCCTCATCCATTTCATCATTCAAATTCAAACCATTTCTTCTTCTTCTTCTTCTGTTGTTTAAATCCCTCTTCTTCTTCAATCATGGTGGTTCAATCCCAACTTGTGTTCGACCATTTTGCTCCCATCAAATCCTGCAAACGCACTGCTCTGTTCACCGGAATTCCGGTGATCGATCTTAAAGACCCAGAAGCCAAAACCCACATCGTCAAAGCCTGTGAGGATTTCGGTTTCTTCAAGGTCGCCAACCATGGCGTCTCCGACGACCTCATCGGCGCTCTCGAAGCCCAAGCTCTGAAATTCTTCCATCTCCCTCAATCGGAGAAGGAAAAAGCAGGGCCACCCGACCCCTTTGGATATGGCAGCAAGAGAATTGGCCCAAACGGCGATGTGGGTTGGATTGAATATGTTCTCCTCAATACCAATCCTCAACTCATTTCCGAAAAATCCTTCTCCATTTTCCGGGAAAATCCCGAGATTTTCCGGTGTTTGGTGGAGGAATATATATTGGCTGTGAAGGAGATGGCTTGTGAAGTGCTGGAAGTTATGGCAGAAGGGCTGAGGATTGAGAAAAGTGCTTTGAGCAGGCTGTTGAAAGATGAAAGGAGTGATTGTTGTTTCAGGCTAAACCATTATCCGCCATGCCCGGAGCTTGAAGCTTTGAGTGGCAGGAATTTGATAGGGTTTGGGGAACATACAGACCCACAAATCATATCTGTCTTGAGATCAAATAACACTACTGGACTTCATATCTGTTTGAGAGATGGAAATTGGGTTTCAGTTCCTCCTGATCACACTTCCTTTTTCATCAATGTGGGTGATTCATTACAGGTAATGACTAATGGGAGGTTTAAGAGTGTAAAGCACAAGGTTTTAGCTGATCCAATAAAATCAAGGTTTTCAATGATCTACTTTGGTGGACCACCTTTGAATGAAAAGATTGCACCTTTACCATCACTATTGGAAGATGGAGAGCAAAGTTTGTACAGAGAATTCACTTGGTGGGATTACAAAAACTCTGCTTATAAATCAAGACTTGCTGATTGTAGGTTAGGCCCTTTTGAGATAAAAAACCTCACCCCTTTGTTGACCTGA

Protein sequence

MPPPPHPFHHSNSNHFFFFFFCCLNPSSSSIMVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTHIVKACEDFGFFKVANHGVSDDLIGALEAQALKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNPQLISEKSFSIFRENPEIFRCLVEEYILAVKEMACEVLEVMAEGLRIEKSALSRLLKDERSDCCFRLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSFFINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEDGEQSLYREFTWWDYKNSAYKSRLADCRLGPFEIKNLTPLLT
Homology
BLAST of Bhi08G001697 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 427.2 bits (1097), Expect = 1.4e-119
Identity = 205/331 (61.93%), Postives = 260/331 (78.55%), Query Frame = 0

Query: 31  IMVVQSQLVFDHFAPIKSCKRTALFT--GIPVIDLKDPEAKTHIVKACEDFGFFKVANHG 90
           +++ Q   + +H + I + K   + T   IPV++L DPEAKT IVKACE+FGFFKV NHG
Sbjct: 2   VVLPQPVTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNHG 61

Query: 91  VSDDLIGALEAQALKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNPQL 150
           V  +L+  LE +A+ FF LPQS K +AGPP+P+GYG+KRIGPNGDVGWIEY+LLN NPQL
Sbjct: 62  VRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQL 121

Query: 151 ISEKSFSIFRENPEIFRCLVEEYILAVKEMACEVLEVMAEGLRIE-KSALSRLLKDERSD 210
            S K+ ++FR+ P+IFR  VEEY+  +KE++ +VLE++AE L IE +  LS++L+DE+SD
Sbjct: 122 SSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSD 181

Query: 211 CCFRLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPD 270
            C RLNHYP   E      +  +GFGEHTDPQIISVLRSNNT GL IC++DG+WV+VPPD
Sbjct: 182 SCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPD 241

Query: 271 HTSFFINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEDG 330
           H+SFFINVGD+LQVMTNGRFKSVKH+VLAD  +SR SMIYFGGPPL++KIAPLP L+ + 
Sbjct: 242 HSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVPEQ 301

Query: 331 EQSLYREFTWWDYKNSAYKSRLADCRLGPFE 359
           +  LY+EFTW  YK+SAYKS+L D RLG FE
Sbjct: 302 DDWLYKEFTWSQYKSSAYKSKLGDYRLGLFE 330

BLAST of Bhi08G001697 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 386.0 bits (990), Expect = 3.5e-107
Identity = 189/302 (62.58%), Postives = 228/302 (75.50%), Query Frame = 0

Query: 58  IPVIDLKDPEAKTHIVKACEDFGFFKVANHGVSDDLIGALEAQALKFFHLPQSEKEKAGP 117
           IPVIDL D +AKT IVKACE+FGFFKV NHGV  DL+  LE +A+ FF L  S K+KAGP
Sbjct: 27  IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDKAGP 86

Query: 118 PDPFGYGSKRIGPNGDVGWIEYVLLNTNPQLISEKSFSIFRENPEIFRCLVEEYILAVKE 177
           PDPFGYG+KRIGPNGD+GW+EY+LLN N  L S K+ +IFR  P IFR  VEEYI  +K 
Sbjct: 87  PDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYIKEMKR 146

Query: 178 MACEVLEVMAEGLRIE-KSALSRLLKDERSDCCFRLNHYPPCPELEALSGRNLIGFGEHT 237
           M+ + LE++ E L+IE K  LSRL+K + SD C R+NHY   PE E    +  IGFGEHT
Sbjct: 147 MSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHY---PEKEETPVKEEIGFGEHT 206

Query: 238 DPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSFFINVGDSLQVMTNGRFKSVKHKVLA 297
           DPQ+IS+LRSN+T GL IC++DG WV V PDH+SFF+ VGD+LQVMTNGRFKSVKH+V+ 
Sbjct: 207 DPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRVVT 266

Query: 298 DPIKSRFSMIYFGGPPLNEKIAPLPSLLEDGEQSLYREFTWWDYKNSAYKSRLADCRLGP 357
           +  +SR SMIYF GPPL+EKIAPL  L+   +  LY EFTW  YK SAYK++L D RLG 
Sbjct: 267 NTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQYKLSAYKTKLGDYRLGL 325

Query: 358 FE 359
           FE
Sbjct: 327 FE 325

BLAST of Bhi08G001697 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 375.6 bits (963), Expect = 4.7e-104
Identity = 183/315 (58.10%), Postives = 233/315 (73.97%), Query Frame = 0

Query: 55  FTGIPVIDLKDPEAKTHIVKACEDFGFFKVANHGVSDDLIGALEAQALKFFHLPQSEKEK 114
           F+ IPVID+ DPE+K  +VKACEDFGFFKV NHGVS +L+  LE + + FF LP+SEK +
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 115 AGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNPQLISEKSFSIFRENPEIFRCLVEEYILA 174
                PFGYG+ +IG NGDVGW+EY+L+N N    S   F    ++P  FR  +EEY  +
Sbjct: 75  VA-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTS 134

Query: 175 VKEMACEVLEVMAEGLRIE-KSALSRLLKDERSDCCFRLNHYPPCP--ELEALSGRNLIG 234
           V++M  +VLE + +GL I+ ++ LS+L+ D+ +D   RLNHYPPCP    +   G+N+IG
Sbjct: 135 VRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVIG 194

Query: 235 FGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSFFINVGDSLQVMTNGRFKSVK 294
           FGEHTDPQIISVLRSNNT+GL I L DG+W+SVPPDHTSFF NVGDSLQVMTNGRFKSV+
Sbjct: 195 FGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSVR 254

Query: 295 HKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEDGEQSLYREFTWWDYKNSAYKSRLAD 354
           H+VLA+  KSR SMIYF GP L ++IAPL  L+++ ++ LY EFTW +YKNS Y SRL+D
Sbjct: 255 HRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLSD 314

Query: 355 CRLGPFEIKNLTPLL 367
            RL  FE K +  LL
Sbjct: 315 NRLQQFERKTIKNLL 328

BLAST of Bhi08G001697 vs. TAIR 10
Match: AT1G30040.2 (gibberellin 2-oxidase )

HSP 1 Score: 313.5 bits (802), Expect = 2.2e-85
Identity = 151/254 (59.45%), Postives = 196/254 (77.17%), Query Frame = 0

Query: 31  IMVVQSQLVFDHFAPIKSCKRTALFT--GIPVIDLKDPEAKTHIVKACEDFGFFKVANHG 90
           +++ Q   + +H + I + K   + T   IPV++L DPEAKT IVKACE+FGFFKV NHG
Sbjct: 2   VVLPQPVTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNHG 61

Query: 91  VSDDLIGALEAQALKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNPQL 150
           V  +L+  LE +A+ FF LPQS K +AGPP+P+GYG+KRIGPNGDVGWIEY+LLN NPQL
Sbjct: 62  VRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQL 121

Query: 151 ISEKSFSIFRENPEIFRCLVEEYILAVKEMACEVLEVMAEGLRIE-KSALSRLLKDERSD 210
            S K+ ++FR+ P+IFR  VEEY+  +KE++ +VLE++AE L IE +  LS++L+DE+SD
Sbjct: 122 SSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSD 181

Query: 211 CCFRLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPD 270
            C RLNHYP   E      +  +GFGEHTDPQIISVLRSNNT GL IC++DG+WV+VPPD
Sbjct: 182 SCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPD 241

Query: 271 HTSFFINVGDSLQV 282
           H+SFFINVGD+LQV
Sbjct: 242 HSSFFINVGDALQV 253

BLAST of Bhi08G001697 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 299.3 bits (765), Expect = 4.3e-81
Identity = 152/305 (49.84%), Postives = 201/305 (65.90%), Query Frame = 0

Query: 59  PVID--LKD-PEAKTHIVKACEDFGFFKVANHGVSDDLIGALEAQALKFFHLPQSEKEKA 118
           PVID  L D  +    IVKACE  GFFKV NHGV  ++I   E +  +FF+ P+S+K +A
Sbjct: 25  PVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEHEGEEFFNKPESDKLRA 84

Query: 119 GPPDPFGYGSKRIGPNGDVGWIEYVLLNTNPQLISEKSFSIFRENPEIFRCLVEEYILAV 178
           GP  PFGYG K IG NGD+G +EY+LL+ NP  +++KS +I  ++P  F     +YI  V
Sbjct: 85  GPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHDDPFKFSSATNDYIRTV 144

Query: 179 KEMACEVLEVMAEGLRIEKSA-LSRLLKDERSDCCFRLNHYPPCPELEALSGRNLIGFGE 238
           +++ACE++++  E L  +KS+ +S L++D RSD   RLNHYPP P   ALSG   IGFGE
Sbjct: 145 RDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPAP--YALSGVGQIGFGE 204

Query: 239 HTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSFFINVGDSLQVMTNGRFKSVKHKV 298
           H+DPQI++VLRSN+  GL IC RDG W+ +P D T FF+ VGD LQ +TNGRF SV+H+V
Sbjct: 205 HSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVLVGDCLQALTNGRFTSVRHRV 264

Query: 299 LADPIKS-RFSMIYFGGPPLNEKIAPLPSLLEDGEQSLYREFTWWDYKNSAYKSRLADCR 358
           LA+  K  R S +YF  PPL  KI+PLP ++       Y  FTW DYK + Y  RL   R
Sbjct: 265 LANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRYNSFTWGDYKKATYSLRLDVPR 324

BLAST of Bhi08G001697 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 3.3e-134
Identity = 231/328 (70.43%), Postives = 267/328 (81.40%), Query Frame = 0

Query: 32  MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTHIVKACEDFGFFKVANHGVSD 91
           MVV SQ   + F  +K  K T LFTGIPV+DL  P+AK  IV AC DFGFFK+ NHGV  
Sbjct: 1   MVVLSQPALNQFFLLKPFKSTPLFTGIPVVDLTHPDAKNLIVNACRDFGFFKLVNHGVPL 60

Query: 92  DLIGALEAQALKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNPQLISE 151
           +L+  LE +AL+FF   QSEK++AGPPDPFGYGSKRIGPNGDVGW+EY+LLNTNP +IS 
Sbjct: 61  ELMANLENEALRFFKKSQSEKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDVISP 120

Query: 152 KSFSIFRENPEIFRCLVEEYILAVKEMACEVLEVMAEGLRI-EKSALSRLLKDERSDCCF 211
           KS  IFRENP  FR +VE YI AVK M   VLE+MAEGL I +++ LSRLLKDE+SD CF
Sbjct: 121 KSLCIFRENPHHFRAVVENYITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSDSCF 180

Query: 212 RLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTS 271
           RLNHYPPCPE++AL+ RNL+GFGEHTDPQIISVLRSN+T+GL ICL DG WVSVPPD TS
Sbjct: 181 RLNHYPPCPEVQALN-RNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPDQTS 240

Query: 272 FFINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEDGEQS 331
           FFINVGD+LQVMTNGRFKSVKH+VLAD  KSR SMIYFGGP L+E IAPLPS++  GE+ 
Sbjct: 241 FFINVGDALQVMTNGRFKSVKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVMLKGEEC 300

Query: 332 LYREFTWWDYKNSAYKSRLADCRLGPFE 359
           LY+EFTW +YK +AY SRLAD RL PF+
Sbjct: 301 LYKEFTWCEYKKAAYTSRLADNRLAPFQ 327

BLAST of Bhi08G001697 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 3.0e-119
Identity = 204/328 (62.20%), Postives = 265/328 (80.79%), Query Frame = 0

Query: 32  MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTHIVKACEDFGFFKVANHGVSD 91
           MV+ S+   + +  +++       + IP++DL  P+AKT IVKACEDFGFFKV NHG+  
Sbjct: 1   MVLLSKPTSEQYTYVRNNMPITFSSSIPLVDLSKPDAKTLIVKACEDFGFFKVINHGIPL 60

Query: 92  DLIGALEAQALKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNPQLISE 151
           D I  LE++A KFF LPQ+EKEKAGP +PFGYG+KRIG NGD+GWIEY+LL TN     +
Sbjct: 61  DAISQLESEAFKFFSLPQTEKEKAGPANPFGYGNKRIGLNGDIGWIEYLLLTTN----QD 120

Query: 152 KSFSIFRENPEIFRCLVEEYILAVKEMACEVLEVMAEGLRIE-KSALSRLLKDERSDCCF 211
            +FS++ E+ + FR L+++Y  A++ MACE+L++MAEGL+I+ K+  S+L+ D++SDC F
Sbjct: 121 HNFSLYGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCLF 180

Query: 212 RLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTS 271
           R+NHYP CPEL A++G NLIGFGEHTDPQIIS+LRSNNT+G  I LRDG+W+SVPPDH+S
Sbjct: 181 RVNHYPACPEL-AINGENLIGFGEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHSS 240

Query: 272 FFINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEDGEQS 331
           FFINVGDSLQVMTNGRFKSV+H+VLA+ I  R SMIYF GPPL+EKIAPLPSL++ G++S
Sbjct: 241 FFINVGDSLQVMTNGRFKSVRHRVLANGIDPRLSMIYFCGPPLSEKIAPLPSLMK-GKES 300

Query: 332 LYREFTWWDYKNSAYKSRLADCRLGPFE 359
           LY+EFTW++YK+S Y SRLAD RLG +E
Sbjct: 301 LYKEFTWFEYKSSTYGSRLADNRLGNYE 322

BLAST of Bhi08G001697 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 1.9e-118
Identity = 205/331 (61.93%), Postives = 260/331 (78.55%), Query Frame = 0

Query: 31  IMVVQSQLVFDHFAPIKSCKRTALFT--GIPVIDLKDPEAKTHIVKACEDFGFFKVANHG 90
           +++ Q   + +H + I + K   + T   IPV++L DPEAKT IVKACE+FGFFKV NHG
Sbjct: 2   VVLPQPVTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNHG 61

Query: 91  VSDDLIGALEAQALKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNPQL 150
           V  +L+  LE +A+ FF LPQS K +AGPP+P+GYG+KRIGPNGDVGWIEY+LLN NPQL
Sbjct: 62  VRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQL 121

Query: 151 ISEKSFSIFRENPEIFRCLVEEYILAVKEMACEVLEVMAEGLRIE-KSALSRLLKDERSD 210
            S K+ ++FR+ P+IFR  VEEY+  +KE++ +VLE++AE L IE +  LS++L+DE+SD
Sbjct: 122 SSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSD 181

Query: 211 CCFRLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPD 270
            C RLNHYP   E      +  +GFGEHTDPQIISVLRSNNT GL IC++DG+WV+VPPD
Sbjct: 182 SCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPD 241

Query: 271 HTSFFINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEDG 330
           H+SFFINVGD+LQVMTNGRFKSVKH+VLAD  +SR SMIYFGGPPL++KIAPLP L+ + 
Sbjct: 242 HSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVPEQ 301

Query: 331 EQSLYREFTWWDYKNSAYKSRLADCRLGPFE 359
           +  LY+EFTW  YK+SAYKS+L D RLG FE
Sbjct: 302 DDWLYKEFTWSQYKSSAYKSKLGDYRLGLFE 330

BLAST of Bhi08G001697 vs. ExPASy Swiss-Prot
Match: O64692 (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 4.9e-106
Identity = 189/302 (62.58%), Postives = 228/302 (75.50%), Query Frame = 0

Query: 58  IPVIDLKDPEAKTHIVKACEDFGFFKVANHGVSDDLIGALEAQALKFFHLPQSEKEKAGP 117
           IPVIDL D +AKT IVKACE+FGFFKV NHGV  DL+  LE +A+ FF L  S K+KAGP
Sbjct: 27  IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDKAGP 86

Query: 118 PDPFGYGSKRIGPNGDVGWIEYVLLNTNPQLISEKSFSIFRENPEIFRCLVEEYILAVKE 177
           PDPFGYG+KRIGPNGD+GW+EY+LLN N  L S K+ +IFR  P IFR  VEEYI  +K 
Sbjct: 87  PDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYIKEMKR 146

Query: 178 MACEVLEVMAEGLRIE-KSALSRLLKDERSDCCFRLNHYPPCPELEALSGRNLIGFGEHT 237
           M+ + LE++ E L+IE K  LSRL+K + SD C R+NHY   PE E    +  IGFGEHT
Sbjct: 147 MSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHY---PEKEETPVKEEIGFGEHT 206

Query: 238 DPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSFFINVGDSLQVMTNGRFKSVKHKVLA 297
           DPQ+IS+LRSN+T GL IC++DG WV V PDH+SFF+ VGD+LQVMTNGRFKSVKH+V+ 
Sbjct: 207 DPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRVVT 266

Query: 298 DPIKSRFSMIYFGGPPLNEKIAPLPSLLEDGEQSLYREFTWWDYKNSAYKSRLADCRLGP 357
           +  +SR SMIYF GPPL+EKIAPL  L+   +  LY EFTW  YK SAYK++L D RLG 
Sbjct: 267 NTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQYKLSAYKTKLGDYRLGL 325

Query: 358 FE 359
           FE
Sbjct: 327 FE 325

BLAST of Bhi08G001697 vs. ExPASy Swiss-Prot
Match: Q8LEA2 (Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 SV=2)

HSP 1 Score: 375.6 bits (963), Expect = 6.6e-103
Identity = 183/315 (58.10%), Postives = 233/315 (73.97%), Query Frame = 0

Query: 55  FTGIPVIDLKDPEAKTHIVKACEDFGFFKVANHGVSDDLIGALEAQALKFFHLPQSEKEK 114
           F+ IPVID+ DPE+K  +VKACEDFGFFKV NHGVS +L+  LE + + FF LP+SEK +
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 115 AGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNPQLISEKSFSIFRENPEIFRCLVEEYILA 174
                PFGYG+ +IG NGDVGW+EY+L+N N    S   F    ++P  FR  +EEY  +
Sbjct: 75  VA-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTS 134

Query: 175 VKEMACEVLEVMAEGLRIE-KSALSRLLKDERSDCCFRLNHYPPCP--ELEALSGRNLIG 234
           V++M  +VLE + +GL I+ ++ LS+L+ D+ +D   RLNHYPPCP    +   G+N+IG
Sbjct: 135 VRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVIG 194

Query: 235 FGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSFFINVGDSLQVMTNGRFKSVK 294
           FGEHTDPQIISVLRSNNT+GL I L DG+W+SVPPDHTSFF NVGDSLQVMTNGRFKSV+
Sbjct: 195 FGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSVR 254

Query: 295 HKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEDGEQSLYREFTWWDYKNSAYKSRLAD 354
           H+VLA+  KSR SMIYF GP L ++IAPL  L+++ ++ LY EFTW +YKNS Y SRL+D
Sbjct: 255 HRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLSD 314

Query: 355 CRLGPFEIKNLTPLL 367
            RL  FE K +  LL
Sbjct: 315 NRLQQFERKTIKNLL 328

BLAST of Bhi08G001697 vs. ExPASy TrEMBL
Match: S0BE08 (GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox3 PE=2 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 4.5e-187
Identity = 313/336 (93.15%), Postives = 327/336 (97.32%), Query Frame = 0

Query: 32  MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTHIVKACEDFGFFKVANHGVSD 91
           MVVQSQLVFDHFAPIKSCKRTALFTGIPVI+LKDPEAK  IVKACEDFGFFK+ NHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVINLKDPEAKIQIVKACEDFGFFKLVNHGVSD 60

Query: 92  DLIGALEAQALKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNPQLISE 151
           +LI ALE+Q LKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEY+LLNTNPQLIS+
Sbjct: 61  ELIAALESQTLKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQ 120

Query: 152 KSFSIFRENPEIFRCLVEEYILAVKEMACEVLEVMAEGLRIEKSALSRLLKDERSDCCFR 211
           KS SIF ENPEIFRCLVEEYI A+KEMAC+VLE+MAEGL+IEKSALS+LLKDERSDCCFR
Sbjct: 121 KSISIFHENPEIFRCLVEEYISAMKEMACQVLELMAEGLKIEKSALSKLLKDERSDCCFR 180

Query: 212 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF 271
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF 240

Query: 272 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEDGEQSL 331
           FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLE+GE+SL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEKSL 300

Query: 332 YREFTWWDYKNSAYKSRLADCRLGPFEIKNLTPLLT 368
           YREFTWWDYK+SAYKSRLADCRL PFEIKNLTPLLT
Sbjct: 301 YREFTWWDYKSSAYKSRLADCRLTPFEIKNLTPLLT 336

BLAST of Bhi08G001697 vs. ExPASy TrEMBL
Match: A0A1S3BA53 (gibberellin 2-beta-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103487682 PE=3 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 2.5e-185
Identity = 311/336 (92.56%), Postives = 324/336 (96.43%), Query Frame = 0

Query: 32  MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTHIVKACEDFGFFKVANHGVSD 91
           MVVQSQLVFDHFAPIKSCKRTALFTGIPVI+ KDPEAKT IVKACEDFGFFK+ NHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVINPKDPEAKTQIVKACEDFGFFKLVNHGVSD 60

Query: 92  DLIGALEAQALKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNPQLISE 151
           +LI ALE++ LKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEY+LLNT PQLIS+
Sbjct: 61  ELIAALESETLKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTKPQLISQ 120

Query: 152 KSFSIFRENPEIFRCLVEEYILAVKEMACEVLEVMAEGLRIEKSALSRLLKDERSDCCFR 211
           KS SIF ENP+IFR LVEEYI A+KEMACEVLE+MAEGL+IEKSALS+LLKDERSDCCFR
Sbjct: 121 KSISIFHENPDIFRSLVEEYISAMKEMACEVLELMAEGLKIEKSALSKLLKDERSDCCFR 180

Query: 212 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF 271
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF 240

Query: 272 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEDGEQSL 331
           FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLE+GEQSL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300

Query: 332 YREFTWWDYKNSAYKSRLADCRLGPFEIKNLTPLLT 368
           YREFTWWDYKNSAYKSRL DCRL PFEIKNLTPLLT
Sbjct: 301 YREFTWWDYKNSAYKSRLGDCRLTPFEIKNLTPLLT 336

BLAST of Bhi08G001697 vs. ExPASy TrEMBL
Match: A0A5A7UKB4 (Gibberellin 2-beta-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold318G00270 PE=3 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 2.5e-185
Identity = 311/336 (92.56%), Postives = 324/336 (96.43%), Query Frame = 0

Query: 32  MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTHIVKACEDFGFFKVANHGVSD 91
           MVVQSQLVFDHFAPIKSCKRTALFTGIPVI+ KDPEAKT IVKACEDFGFFK+ NHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVINPKDPEAKTQIVKACEDFGFFKLVNHGVSD 60

Query: 92  DLIGALEAQALKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNPQLISE 151
           +LI ALE++ LKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEY+LLNT PQLIS+
Sbjct: 61  ELIAALESETLKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTKPQLISQ 120

Query: 152 KSFSIFRENPEIFRCLVEEYILAVKEMACEVLEVMAEGLRIEKSALSRLLKDERSDCCFR 211
           KS SIF ENP+IFR LVEEYI A+KEMACEVLE+MAEGL+IEKSALS+LLKDERSDCCFR
Sbjct: 121 KSISIFHENPDIFRSLVEEYISAMKEMACEVLELMAEGLKIEKSALSKLLKDERSDCCFR 180

Query: 212 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF 271
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF 240

Query: 272 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEDGEQSL 331
           FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLE+GEQSL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300

Query: 332 YREFTWWDYKNSAYKSRLADCRLGPFEIKNLTPLLT 368
           YREFTWWDYKNSAYKSRL DCRL PFEIKNLTPLLT
Sbjct: 301 YREFTWWDYKNSAYKSRLGDCRLTPFEIKNLTPLLT 336

BLAST of Bhi08G001697 vs. ExPASy TrEMBL
Match: I0J1I9 (Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox3 PE=2 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 2.1e-184
Identity = 314/336 (93.45%), Postives = 319/336 (94.94%), Query Frame = 0

Query: 32  MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTHIVKACEDFGFFKVANHGVSD 91
           MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKT IVKACEDFGFFKV NHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTQIVKACEDFGFFKVINHGVSD 60

Query: 92  DLIGALEAQALKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNPQLISE 151
            LIG LEAQALKFF LPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTN +LISE
Sbjct: 61  HLIGDLEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNHELISE 120

Query: 152 KSFSIFRENPEIFRCLVEEYILAVKEMACEVLEVMAEGLRIEKSALSRLLKDERSDCCFR 211
           KS  I RENPEIFRC VEEYILAVKEMACEVLE MA GLRIEKSALSRLLKDERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMALGLRIEKSALSRLLKDERSDCCFR 180

Query: 212 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF 271
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVL+SNNT GLHICLRDGNWVSVPPD+ SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLKSNNTAGLHICLRDGNWVSVPPDNASF 240

Query: 272 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEDGEQSL 331
           FINVGDSLQVMTNGRFKSVKHKVLADPIKSR SMIYFGGPPLNEKIAPLPSL+E+GEQSL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRLSMIYFGGPPLNEKIAPLPSLMEEGEQSL 300

Query: 332 YREFTWWDYKNSAYKSRLADCRLGPFEIKNLTPLLT 368
           YREFTWWDYK SAYKSRLADCRLGPFEIK LTPLLT
Sbjct: 301 YREFTWWDYKTSAYKSRLADCRLGPFEIKTLTPLLT 336

BLAST of Bhi08G001697 vs. ExPASy TrEMBL
Match: A0A6J1EMS7 (gibberellin 2-beta-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111433993 PE=3 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 4.6e-184
Identity = 313/336 (93.15%), Postives = 319/336 (94.94%), Query Frame = 0

Query: 32  MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTHIVKACEDFGFFKVANHGVSD 91
           MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKT IVKACEDFGFFKV NHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTQIVKACEDFGFFKVINHGVSD 60

Query: 92  DLIGALEAQALKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNPQLISE 151
            LIG LEAQALKFF LPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTN +LISE
Sbjct: 61  HLIGDLEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNHELISE 120

Query: 152 KSFSIFRENPEIFRCLVEEYILAVKEMACEVLEVMAEGLRIEKSALSRLLKDERSDCCFR 211
           KS  I RENPE+FRC VEEYILAVKEMACEVLE MA GLRIEKSALS LLKDERSDCCFR
Sbjct: 121 KSLFILRENPEVFRCAVEEYILAVKEMACEVLEAMALGLRIEKSALSWLLKDERSDCCFR 180

Query: 212 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF 271
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVL+SNNT GLHICLRDGNWVSVPPD+ SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLKSNNTAGLHICLRDGNWVSVPPDNASF 240

Query: 272 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEDGEQSL 331
           FINVGDSLQVMTNGRFKSVKHKVLADPIKSR SMIYFGGPPLNEKIAPLPSL+E+GEQSL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRLSMIYFGGPPLNEKIAPLPSLMEEGEQSL 300

Query: 332 YREFTWWDYKNSAYKSRLADCRLGPFEIKNLTPLLT 368
           YREFTWWDYKNSAYKSRLADCRLGPFEIK LTPLLT
Sbjct: 301 YREFTWWDYKNSAYKSRLADCRLGPFEIKTLTPLLT 336

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G30040.11.4e-11961.93gibberellin 2-oxidase [more]
AT2G34555.13.5e-10762.58gibberellin 2-oxidase 3 [more]
AT1G78440.14.7e-10458.10Arabidopsis thaliana gibberellin 2-oxidase 1 [more]
AT1G30040.22.2e-8559.45gibberellin 2-oxidase [more]
AT1G02400.14.3e-8149.84gibberellin 2-oxidase 6 [more]
Match NameE-valueIdentityDescription
Q9XG833.3e-13470.43Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=... [more]
Q9SQ803.0e-11962.20Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1[more]
Q9XFR91.9e-11861.93Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ... [more]
O646924.9e-10662.58Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 ... [more]
Q8LEA26.6e-10358.10Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 ... [more]
Match NameE-valueIdentityDescription
S0BE084.5e-18793.15GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox3 PE=2 SV=1[more]
A0A1S3BA532.5e-18592.56gibberellin 2-beta-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103487682 PE=3... [more]
A0A5A7UKB42.5e-18592.56Gibberellin 2-beta-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
I0J1I92.1e-18493.45Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox3 PE=2 SV=1[more]
A0A6J1EMS74.6e-18493.15gibberellin 2-beta-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC11143399... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00682IPNSYNTHASEcoord: 72..89
score: 55.56
coord: 235..256
score: 27.69
coord: 271..297
score: 29.29
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 37..361
NoneNo IPR availablePANTHERPTHR47990:SF121GIBBERELLIN 2-BETA-DIOXYGENASE 2coord: 37..361
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 42..350
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 30..351
e-value: 1.2E-80
score: 273.3
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 58..125
e-value: 1.0E-15
score: 58.5
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 207..309
e-value: 1.7E-25
score: 89.5
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 205..311
score: 10.839873

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi08M001697Bhi08M001697mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045487 gibberellin catabolic process
biological_process GO:0009416 response to light stimulus
molecular_function GO:0052634 C-19 gibberellin 2-beta-dioxygenase activity
molecular_function GO:0046872 metal ion binding