Bhi08G000856 (gene) Wax gourd (B227) v1

Overview


Name	Bhi08G000856
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Pectinesterase
Location	chr8: 33487759 .. 33490141 (-)
RNA-Seq Expression	Bhi08G000856
Synteny	Bhi08G000856

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCAAAAACATTCTTCCCATACAACAAGTTTGAGGAATTAACATCAACATGTCTTTGCTTCGACCTTACGGTAAGGTCGACGAAACCGACCAATTGAGGCTCGACGCTAGCCGGAGGACTCGGAAGAGGATTGCTGTCATTGTTTTGTCCTCTGTTGTTCTTGTTGGTGTTGTTGTTGCTGTCGTGTTTGGTACTAGAAATGCTTCCAATAACAATGATGACAGTAACAATCAAAGTGGTGCTCTTTCAACTTCAATCAAAGCAGTTTGTGATGTGACATTGTATCCTGTTACATGTCAAAATGCTTTTGGCCCTTTAGCTAACTCGAGCCACCTCGACCCCAGTCAGATATCCAAGCTTTCGGTTCAATTGGCGCTCGGTGAATTGTCGCGAGTTGCTGATTACATTTTTTATCATGCTATTACCAATTCAACTGACAATAAGACCATATTGGCTTTGAAAAATTGTCATGAACTTTTGGACTTAGCATTGGATCATTTGAATATCTCATTGTCTTCTAGTGATATTACATTGTTGAAAGCTGTGGATGATTTGAAAACATGGCTAACATCAGCTGCTACTTACCAACAGACTTGCATTGACGGTCTCACCGAAGTCGATCCGGCTTTGGCAAACCTCATTGCAAACTACTTGAAGAACTCCACTGAACTGACTAGTAATGGACTCGCCATCGTGTCATTCTTTTCGAAGCTTACGGATTCTTTAAGTCTACGACGATTGATGAGTTATGAAGATCACCAAAGCAATGGAGATTGGGCACGCCCGATACTCCGAAAGCTGGCTCAGAGAGATATCAGGAAGCAGGCTGACATTGTCGTAGCGAAAGACAGTTCGGGGAAGTACAAAACAATCACTGCAGCTCTCGAGGCTGTTCCTAATAAGAGCAAGAAGAGAACTGTGATTTATGTGAAGAAGGGAACTTACAAGGAGAATGTTGAAGTTGTGAAGAACAAATGGAATGTGGTAATTGTTGGTGATGGAATGACTGCAACCATAGTAACTGGCAGCCTTAATGTCATAGATGGGACACCAACATTTTCAACTGCAACATTTGGTAAGTAAAATAAAGAACAGACTCCTCTAATCTTATAACTTGGAGCTATTTGGAGCTCGGAGCTTTAAGTTGGTTATTATAGTTTGTTGGTTATAATAGTCTGTATTTGAGGTGCAGAATATTTCAATATGAGTTATAATAGTCTGCATTTCGGGTGCATACTATTGTCTGGATTACAAATGAGGAGATAGGATAGTTATAAAAAAGTAAACACTGCAGGTAATGTAGATTTTAGAATAATTTTACTATAGCCAAGTGTGGGTTATAATACTCGGGAACTTCAACTATAATAATAGGAGATTCAGGGCTTATAAATATAACAGCTCACCCCATCTAAGGCAAGTTGAGGTCTAAACACCCTTTCGTTTTTTACAAAGCTTTTACAATTGACAATTCATTTCCATATGCATTTTTTTCTGTTGCAGCTGCAAAGGGAAAAGGGTTCATTGCCATAGATATGGGATTCAGGAACACTGCTGGACCGAACAAACACCAAGCGGTTGCTCTGATGTCGACATCCGACCAATCGATCTTCTACCGGTGCCAAATGGATGCATACCAAGACACTCTCTACTCACACTCCAACCGCCAATTCTACCGTGAATGCAAGGTCTACGGCACGGTCGATTTCATCTTTGGAAACTCAGCAGTAGTCCTACAAAACTGCACCATTGCACCCAGGTTGCCTCTTTTGGGCCAAAAGAACACAATCACTGCTCAAGGCAGGTTTGATCCTAATCAAAACACTGGCATTTCGATACAAGACTCGTCGATCCAACCATACGAGAACTTGAAAACTACAGAAACTTACCTCGGCCGTCCGTGGAAAAACTACTCAACGACCGTGTTTATGCAAAGTAATTTGGGGAGTTTGATTCACCCAAGTGGCTGGTTGCCTTGGGTTGGAACATCAGCACCAGACACCATATTCTATGCAGAGTTTCAAAACTATGGCCCTGGTTCTTCCACAGCTAAAAGAGTTAAGTGGACGGGTGTGAAGAACATTGACATTAAAACTGCAAAGAAGTTCACAGTTTCTTCCTTCATTGATGGTAAAGATTGGATCTCAAAGGCACAAGTTCCCTTCAAAGCTAGCATTTGATTTTGATTTGGTAAAGCTTGATGGAGTTTTTTTTTCCTCTCATTGCTACAAATGCCACTAAATGCTTTTGTCTTCAAACTTTGTTGTTTCTTTTTCTATTTTGTTTCATATTGTTCAGTTTTTTTATACTTCCTTTTAACCATTTGATTGATTGGTTTTGTAATAAGAACATATACTAAGAAAGTTCTGTCCACTTTTAGGGTTGAG

mRNA sequence

GCAAAAACATTCTTCCCATACAACAAGTTTGAGGAATTAACATCAACATGTCTTTGCTTCGACCTTACGGTAAGGTCGACGAAACCGACCAATTGAGGCTCGACGCTAGCCGGAGGACTCGGAAGAGGATTGCTGTCATTGTTTTGTCCTCTGTTGTTCTTGTTGGTGTTGTTGTTGCTGTCGTGTTTGGTACTAGAAATGCTTCCAATAACAATGATGACAGTAACAATCAAAGTGGTGCTCTTTCAACTTCAATCAAAGCAGTTTGTGATGTGACATTGTATCCTGTTACATGTCAAAATGCTTTTGGCCCTTTAGCTAACTCGAGCCACCTCGACCCCAGTCAGATATCCAAGCTTTCGGTTCAATTGGCGCTCGGTGAATTGTCGCGAGTTGCTGATTACATTTTTTATCATGCTATTACCAATTCAACTGACAATAAGACCATATTGGCTTTGAAAAATTGTCATGAACTTTTGGACTTAGCATTGGATCATTTGAATATCTCATTGTCTTCTAGTGATATTACATTGTTGAAAGCTGTGGATGATTTGAAAACATGGCTAACATCAGCTGCTACTTACCAACAGACTTGCATTGACGGTCTCACCGAAGTCGATCCGGCTTTGGCAAACCTCATTGCAAACTACTTGAAGAACTCCACTGAACTGACTAGTAATGGACTCGCCATCGTGTCATTCTTTTCGAAGCTTACGGATTCTTTAAGTCTACGACGATTGATGAGTTATGAAGATCACCAAAGCAATGGAGATTGGGCACGCCCGATACTCCGAAAGCTGGCTCAGAGAGATATCAGGAAGCAGGCTGACATTGTCGTAGCGAAAGACAGTTCGGGGAAGTACAAAACAATCACTGCAGCTCTCGAGGCTGTTCCTAATAAGAGCAAGAAGAGAACTGTGATTTATGTGAAGAAGGGAACTTACAAGGAGAATGTTGAAGTTGTGAAGAACAAATGGAATGTGGTAATTGTTGGTGATGGAATGACTGCAACCATAGTAACTGGCAGCCTTAATGTCATAGATGGGACACCAACATTTTCAACTGCAACATTTGCTGCAAAGGGAAAAGGGTTCATTGCCATAGATATGGGATTCAGGAACACTGCTGGACCGAACAAACACCAAGCGGTTGCTCTGATGTCGACATCCGACCAATCGATCTTCTACCGGTGCCAAATGGATGCATACCAAGACACTCTCTACTCACACTCCAACCGCCAATTCTACCGTGAATGCAAGGTCTACGGCACGGTCGATTTCATCTTTGGAAACTCAGCAGTAGTCCTACAAAACTGCACCATTGCACCCAGGTTGCCTCTTTTGGGCCAAAAGAACACAATCACTGCTCAAGGCAGGTTTGATCCTAATCAAAACACTGGCATTTCGATACAAGACTCGTCGATCCAACCATACGAGAACTTGAAAACTACAGAAACTTACCTCGGCCGTCCGTGGAAAAACTACTCAACGACCGTGTTTATGCAAAGTAATTTGGGGAGTTTGATTCACCCAAGTGGCTGGTTGCCTTGGGTTGGAACATCAGCACCAGACACCATATTCTATGCAGAGTTTCAAAACTATGGCCCTGGTTCTTCCACAGCTAAAAGAGTTAAGTGGACGGGTGTGAAGAACATTGACATTAAAACTGCAAAGAAGTTCACAGTTTCTTCCTTCATTGATGGTAAAGATTGGATCTCAAAGGCACAAGTTCCCTTCAAAGCTAGCATTTGATTTTGATTTGGTAAAGCTTGATGGAGTTTTTTTTTCCTCTCATTGCTACAAATGCCACTAAATGCTTTTGTCTTCAAACTTTGTTGTTTCTTTTTCTATTTTGTTTCATATTGTTCAGTTTTTTTATACTTCCTTTTAACCATTTGATTGATTGGTTTTGTAATAAGAACATATACTAAGAAAGTTCTGTCCACTTTTAGGGTTGAG

Coding sequence (CDS)

ATGTCTTTGCTTCGACCTTACGGTAAGGTCGACGAAACCGACCAATTGAGGCTCGACGCTAGCCGGAGGACTCGGAAGAGGATTGCTGTCATTGTTTTGTCCTCTGTTGTTCTTGTTGGTGTTGTTGTTGCTGTCGTGTTTGGTACTAGAAATGCTTCCAATAACAATGATGACAGTAACAATCAAAGTGGTGCTCTTTCAACTTCAATCAAAGCAGTTTGTGATGTGACATTGTATCCTGTTACATGTCAAAATGCTTTTGGCCCTTTAGCTAACTCGAGCCACCTCGACCCCAGTCAGATATCCAAGCTTTCGGTTCAATTGGCGCTCGGTGAATTGTCGCGAGTTGCTGATTACATTTTTTATCATGCTATTACCAATTCAACTGACAATAAGACCATATTGGCTTTGAAAAATTGTCATGAACTTTTGGACTTAGCATTGGATCATTTGAATATCTCATTGTCTTCTAGTGATATTACATTGTTGAAAGCTGTGGATGATTTGAAAACATGGCTAACATCAGCTGCTACTTACCAACAGACTTGCATTGACGGTCTCACCGAAGTCGATCCGGCTTTGGCAAACCTCATTGCAAACTACTTGAAGAACTCCACTGAACTGACTAGTAATGGACTCGCCATCGTGTCATTCTTTTCGAAGCTTACGGATTCTTTAAGTCTACGACGATTGATGAGTTATGAAGATCACCAAAGCAATGGAGATTGGGCACGCCCGATACTCCGAAAGCTGGCTCAGAGAGATATCAGGAAGCAGGCTGACATTGTCGTAGCGAAAGACAGTTCGGGGAAGTACAAAACAATCACTGCAGCTCTCGAGGCTGTTCCTAATAAGAGCAAGAAGAGAACTGTGATTTATGTGAAGAAGGGAACTTACAAGGAGAATGTTGAAGTTGTGAAGAACAAATGGAATGTGGTAATTGTTGGTGATGGAATGACTGCAACCATAGTAACTGGCAGCCTTAATGTCATAGATGGGACACCAACATTTTCAACTGCAACATTTGCTGCAAAGGGAAAAGGGTTCATTGCCATAGATATGGGATTCAGGAACACTGCTGGACCGAACAAACACCAAGCGGTTGCTCTGATGTCGACATCCGACCAATCGATCTTCTACCGGTGCCAAATGGATGCATACCAAGACACTCTCTACTCACACTCCAACCGCCAATTCTACCGTGAATGCAAGGTCTACGGCACGGTCGATTTCATCTTTGGAAACTCAGCAGTAGTCCTACAAAACTGCACCATTGCACCCAGGTTGCCTCTTTTGGGCCAAAAGAACACAATCACTGCTCAAGGCAGGTTTGATCCTAATCAAAACACTGGCATTTCGATACAAGACTCGTCGATCCAACCATACGAGAACTTGAAAACTACAGAAACTTACCTCGGCCGTCCGTGGAAAAACTACTCAACGACCGTGTTTATGCAAAGTAATTTGGGGAGTTTGATTCACCCAAGTGGCTGGTTGCCTTGGGTTGGAACATCAGCACCAGACACCATATTCTATGCAGAGTTTCAAAACTATGGCCCTGGTTCTTCCACAGCTAAAAGAGTTAAGTGGACGGGTGTGAAGAACATTGACATTAAAACTGCAAAGAAGTTCACAGTTTCTTCCTTCATTGATGGTAAAGATTGGATCTCAAAGGCACAAGTTCCCTTCAAAGCTAGCATTTGA

Protein sequence

MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVGVVVAVVFGTRNASNNNDDSNNQSGALSTSIKAVCDVTLYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELSRVADYIFYHAITNSTDNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWLTSAATYQQTCIDGLTEVDPALANLIANYLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSNGDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSKKRTVIYVKKGTYKENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAAKGKGFIAIDMGFRNTAGPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYGTVDFIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYENLKTTETYLGRPWKNYSTTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWTGVKNIDIKTAKKFTVSSFIDGKDWISKAQVPFKASI

Homology

[Close]

BLAST of Bhi08G000856 vs. TAIR 10
Match: AT3G10710.1 (root hair specific 12 )

HSP 1 Score: 583.6 bits (1503), Expect = 1.8e-166
Identity = 308/569 (54.13%), Postives = 400/569 (70.30%), Query Frame = 0


Query: 6   PYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVGVVVAVVFGT--RNASNNNDDSNNQS 65
           PYGKVDE + +RL+A R+TRK IA+I +S V+L G+V+  VFGT     S    ++NN  
Sbjct: 4   PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63

Query: 66  GALSTSIKAVCDVTLYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELSRVADYIFYH 125
            ++S S+KAVCDVTL+   C    G   N+S L+P ++ + +V++ + E+S+       +
Sbjct: 64  DSISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKA-----IN 123

Query: 126 AITNST-DNKTILALKNCHELLDLALDHLNISLSSS---DITLLKAVDDLKTWLTSAATY 185
           A ++S  D K  + +  C ELLDL +D+LN +L+SS   D+T+ + VDDL+TWL+SA TY
Sbjct: 124 AFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGTY 183

Query: 186 QQTCIDGLTEVDPALANLIANYLKNSTELTSNGLAIVSFFSKLTDSLSL-RRLMSYEDHQ 245
           Q+TC++ L    P +     ++LKNSTELTSN LAI+++  K+ DS  L RRL++  D +
Sbjct: 184 QRTCVETLA---PDMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADVE 243

Query: 246 SNGDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSKKRTVIYVKKGT 305
            +    R   R L   D+RK ADIVVAKD SGKY+TI  AL+ VP KS+KRT+IYVKKG 
Sbjct: 244 VDFHAGR---RLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKGV 303

Query: 306 YKENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAAKGKGFIAIDMGFRN 365
           Y ENV+V K  WNV++VGDG + +IV+G LNVIDGTPTF TATFA  GKGF+A DMGF N
Sbjct: 304 YFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFIN 363

Query: 366 TAGPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYGTVDFIFGNSAV 425
           TAGP+KHQAVALM ++D + FYRC M+AYQDTLY H+ RQFYREC + GTVDFIFGNSA 
Sbjct: 364 TAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSAS 423

Query: 426 VLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYENLKTTETYLGRPWKN 485
           VLQ+C I PR P+ GQ+NTITAQGR DPN NTGISI   +I P  +L    T+LGRPWKN
Sbjct: 424 VLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWKN 483

Query: 486 YSTTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWTGVKNIDI 545
           +STTV M S L   I   GWLPW G SAPDTIFY E++N GPG+ST  RVKW G++ +  
Sbjct: 484 FSTTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLST 543

Query: 546 KTAKKFTVSSFIDGKDWISKAQVPFKASI 568
           K A +FTV  FIDG  W+   +VPF++ +
Sbjct: 544 KEANRFTVKPFIDGGRWLPATKVPFRSGL 561

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BLAST of Bhi08G000856 vs. TAIR 10
Match: AT5G04960.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 569.7 bits (1467), Expect = 2.6e-162
Identity = 301/566 (53.18%), Postives = 395/566 (69.79%), Query Frame = 0


Query: 7   YGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVGVVVAVVFGTRNASNNNDDSNNQSG-A 66
           YG++DE +Q +L+ASR+T+KRIA+I +SS+VLV +VV  V GT    N+       +G  
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEP 63

Query: 67  LSTSIKAVCDVTLYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELSRVADYIFYHAI 126
           +S S+KA+CDVTL+   C    G   N+S   P ++ K +V++ + ELS+V D     + 
Sbjct: 64  ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGF---SN 123

Query: 127 TNSTDNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWLTSAATYQQTCID 186
               DN T  A+  C EL+ LA+D LN +++SS    LK  DDL+TWL+S  TYQ+TC+D
Sbjct: 124 GEHMDNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETCMD 183

Query: 187 GLTEVD-PALANLIANYLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSNGDWA 246
            L E + P+L     N+LKNSTE+TSN LAI+++  K+ D++  RR    E   +    A
Sbjct: 184 ALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVVVA 243

Query: 247 -RPIL---RKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSKKRTVIYVKKGTYK 306
             P++   R L   D++K+A IVVAKD SGKY+TI  AL  V  K++K T+IYVKKG Y 
Sbjct: 244 DLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGVYL 303

Query: 307 ENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAAKGKGFIAIDMGFRNTA 366
           ENV V K KWNVV+VGDG + TIV+  LN IDGTPTF TATFA  GKGF+A DMGF NTA
Sbjct: 304 ENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFINTA 363

Query: 367 GPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYGTVDFIFGNSAVVL 426
           GP KHQAVALM ++D S+FY+C MDA+QDT+Y+H+ RQFYR+C + GTVDFIFGN+AVV 
Sbjct: 364 GPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAVVF 423

Query: 427 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYENLKTTETYLGRPWKNYS 486
           Q C I PR P+ GQ+NTITAQGR DPNQNTGISI + +I+P +NL   +T+LGRPWK++S
Sbjct: 424 QKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKDFS 483

Query: 487 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWTGVK-NIDIK 546
           TTV M+S +   I+P GWLPW G +APDTIFYAE+ N GPG+ST  RVKW G+K ++  K
Sbjct: 484 TTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLTKK 543

Query: 547 TAKKFTVSSFIDGKDWISKAQVPFKA 566
            A KFTV  FIDG +W+   +VPF +
Sbjct: 544 EANKFTVKPFIDGNNWLPATKVPFNS 562

[Close]

BLAST of Bhi08G000856 vs. TAIR 10
Match: AT1G53840.1 (pectin methylesterase 1 )

HSP 1 Score: 446.4 bits (1147), Expect = 3.3e-125
Identity = 259/574 (45.12%), Postives = 358/574 (62.37%), Query Frame = 0


Query: 7   YGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVGVVVAVVFGTRNASNNNDDSNNQSGAL 66
           YGKVDE   L L   ++TRKR+ ++ +S VVL+ V++A V  T    N N+ + +    L
Sbjct: 10  YGKVDEAQDLAL--KKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPEL 69

Query: 67  --STSIKAVCDVTLYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELSRVADYIFYHA 126
             STS+KA+C VT +P +C ++   L +S+  DP  + KLS+++ + EL  ++D      
Sbjct: 70  TPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISD--LPEK 129

Query: 127 ITNSTDNKTI-LALKNCHELLDLALDHLNISLSSSDITLLK------AVDDLKTWLTSAA 186
           ++  T+++ I  AL+ C +L++ ALD LN ++S+ D    K       ++DLKTWL++  
Sbjct: 130 LSKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATV 189

Query: 187 TYQQTCIDGLTEV--------DPALANLIANYLKNSTELTSNGLAIVSFFSKLTDSLSL- 246
           T  +TC D L E+        +  +   + + +  STE TSN LAIVS        L + 
Sbjct: 190 TDHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIP 249

Query: 247 ----RRLMSYEDHQSNGDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPN 306
               RRLMS+  HQ + D+ +   R+L Q    K  D+ VA D +G   T+  A+  VP 
Sbjct: 250 IHRRRRLMSHH-HQQSVDFEKWARRRLLQTAGLK-PDVTVAGDGTGDVLTVNEAVAKVPK 309

Query: 307 KSKKRTVIYVKKGTYKENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAA 366
           KS K  VIYVK GTY ENV + K+KWNV+I GDG   TI++GS N +DGTPT+ TATFA 
Sbjct: 310 KSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFAI 369

Query: 367 KGKGFIAIDMGFRNTAGPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECK 426
           +GKGFI  D+G  NTAG  KHQAVA  S SD S++Y+C  D +QDTLY HSNRQFYR+C 
Sbjct: 370 QGKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDCD 429

Query: 427 VYGTVDFIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYEN 486
           V GT+DFIFG++AVV Q C I PR PL  Q NTITAQG+ DPNQ++G+SIQ  +I    N
Sbjct: 430 VTGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISANGN 489

Query: 487 LKTTETYLGRPWKNYSTTVFMQSNLGSLIHPSGWLPWV-GTSAPDTIFYAEFQNYGPGSS 546
           +    TYLGRPWK +STTV M++ +G+++ PSGW+ WV G   P +I Y E++N GPGS 
Sbjct: 490 V-IAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGSD 549

Query: 547 TAKRVKWTGVKNI-DIKTAKKFTVSSFIDGKDWI 557
             +RVKW G K +     A KFTV++ + G DWI
Sbjct: 550 VTQRVKWAGYKPVMSDAEAAKFTVATLLHGADWI 576

[Close]

BLAST of Bhi08G000856 vs. TAIR 10
Match: AT3G14300.1 (pectinesterase family protein )

HSP 1 Score: 395.6 bits (1015), Expect = 6.8e-110
Identity = 236/512 (46.09%), Postives = 309/512 (60.35%), Query Frame = 0


Query: 67  STSIKAVCDVTLYPVTCQNAFGPL-ANSSHLDPSQISKLSVQLALGELSRVADYIFYHAI 126
           S+ ++ VC+VT YP +C ++   L  + +  DP  + +LS+Q+   EL+ +   +     
Sbjct: 456 SSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLFRLSLQVTFDELNSIVG-LPKKLA 515

Query: 127 TNSTDNKTILALKNCHELLDLALDHLNISLSSSDITL--------LKAVDDLKTWLTSAA 186
             + D     AL  C ++ DLA+D +N ++SS D  +           + DL TWL+SA 
Sbjct: 516 EETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVISGGKKNLNSSTIGDLITWLSSAV 575

Query: 187 TYQQTCIDGLTE--VDPALANLIANYLKNSTELTSNGLAIVSFF----SKLTDSLSLRRL 246
           T   TC D L E   +  +   + + + NSTE TSN LAIV+      SK    +  RRL
Sbjct: 576 TDIGTCGDTLDEDNYNSPIPQKLKSAMVNSTEFTSNSLAIVAQVLKKPSKSRIPVQGRRL 635

Query: 247 MSYEDHQSNGDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSKKRTV 306
           +   +  S  +W RP +R+L Q        + VA D SG  +T+  A+  VP K K   V
Sbjct: 636 L---NSNSFPNWVRPGVRRLLQAK-NLTPHVTVAADGSGDVRTVNEAVWRVPKKGKTMFV 695

Query: 307 IYVKKGTYKENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAAKGKGFIA 366
           IYVK GTY ENV + K+KWNV I GDG   TI++GS N++DG  TF+T+TFA +GKGF+ 
Sbjct: 696 IYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDGVRTFNTSTFATEGKGFMM 755

Query: 367 IDMGFRNTAGPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYGTVDF 426
            DMG  NTAGP KHQAVA  S SD+S++YRC  D YQDTLY+HSNRQ+YR C V GTVDF
Sbjct: 756 KDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYTHSNRQYYRNCDVTGTVDF 815

Query: 427 IFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYENLKTTETY 486
           IFG   VV Q C+I PR PL  Q NTITA+G  + NQNTGISI   +I P  N+ T  TY
Sbjct: 816 IFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGISIHQCTISPNGNV-TATTY 875

Query: 487 LGRPWKNYSTTVFMQSNLGSLIHPSGWLPWVGT--SAPDTIFYAEFQNYGPGSSTAKRVK 546
           LGRPWK +S TV MQS +GS ++P+GW+ W  T    P TIFY E++N GPGS  +KRVK
Sbjct: 876 LGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIFYREYKNSGPGSDLSKRVK 935

Query: 547 WTGVKNIDI-KTAKKFTVSSFIDGKD-WISKA 560
           W G K I     A +FTV  F+ G D WI KA
Sbjct: 936 WAGYKPISSDDEAARFTVKYFLRGDDNWIPKA 961

[Close]

BLAST of Bhi08G000856 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 374.4 bits (960), Expect = 1.6e-103
Identity = 226/569 (39.72%), Postives = 326/569 (57.29%), Query Frame = 0


Query: 18  LDASRRTRKRIAVIVLSSVVLVGVVVAVVFGTRNASNNNDDSNNQSGALSTSIKAVCDVT 77
           +D   + +K I   V+++++++ VV   +  +RN S++      Q    +T+++AVC  T
Sbjct: 3   VDGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPT 62

Query: 78  LYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELS---RVADYIFYHAITNSTDNKTI 137
            Y  TC N+    A+     P  + KL   + +  +    + A         N  D K  
Sbjct: 63  DYKETCVNSL-MKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKG- 122

Query: 138 LALKNCHELLDLALDHLNISLSSSD----ITLLKAVDDLKTWLTSAATYQQTCIDGLTEV 197
            AL+ C +L++ A D L   L + D      +   V+DL+ WL+ +  YQQTC+D   E 
Sbjct: 123 -ALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEET 182

Query: 198 DPALANLIANYLKNSTELTSNGLAIVSFFSKLTDSLSL-----------RRLMSYEDHQS 257
           +  L+  +    K S ELTSNGLA+++  S L    ++           R+L+S ED   
Sbjct: 183 NSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAED--G 242

Query: 258 NGDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSKKRTVIYVKKGTY 317
              W  P  R+L       +A++VVA D SG+YKTI  AL AVP  ++K  VIY+K+G Y
Sbjct: 243 IPSWVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVY 302

Query: 318 KENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDG-TPTFSTATFAAKGKGFIAIDMGFRN 377
            E V+V K   +V  +GDG T T +TGSLN   G   T+ TAT A  G  F A ++GF N
Sbjct: 303 NEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFEN 362

Query: 378 TAGPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYGTVDFIFGNSAV 437
           TAGP  HQAVAL  ++D ++FY CQ+D YQDTLY HS+RQF+R+C V GTVDFIFG+  V
Sbjct: 363 TAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIV 422

Query: 438 VLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSI---QPYENLKT-TETYLGR 497
           VLQNC I  R P+  Q   ITAQGR D  ++TG+ +Q+  I     Y  +K+  + YLGR
Sbjct: 423 VLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGR 482

Query: 498 PWKNYSTTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWTGVK 557
           PWK +S T+ M + +  +I P+GWLPW G  A +T++YAE++N GPGS+ A+RVKW G+K
Sbjct: 483 PWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIK 542

Query: 558 NIDIKTAKKFTVSSFIDGKDWISKAQVPF 564
            +  K A +FT + F+ G  WI   +VP+
Sbjct: 543 KLSPKQALRFTPARFLRGNLWIPPNRVPY 566

[Close]

BLAST of Bhi08G000856 vs. ExPASy Swiss-Prot
Match: Q9SG77 (Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3702 GN=PME24 PE=3 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 2.5e-165
Identity = 308/569 (54.13%), Postives = 400/569 (70.30%), Query Frame = 0


Query: 6   PYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVGVVVAVVFGT--RNASNNNDDSNNQS 65
           PYGKVDE + +RL+A R+TRK IA+I +S V+L G+V+  VFGT     S    ++NN  
Sbjct: 4   PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63

Query: 66  GALSTSIKAVCDVTLYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELSRVADYIFYH 125
            ++S S+KAVCDVTL+   C    G   N+S L+P ++ + +V++ + E+S+       +
Sbjct: 64  DSISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKA-----IN 123

Query: 126 AITNST-DNKTILALKNCHELLDLALDHLNISLSSS---DITLLKAVDDLKTWLTSAATY 185
           A ++S  D K  + +  C ELLDL +D+LN +L+SS   D+T+ + VDDL+TWL+SA TY
Sbjct: 124 AFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGTY 183

Query: 186 QQTCIDGLTEVDPALANLIANYLKNSTELTSNGLAIVSFFSKLTDSLSL-RRLMSYEDHQ 245
           Q+TC++ L    P +     ++LKNSTELTSN LAI+++  K+ DS  L RRL++  D +
Sbjct: 184 QRTCVETLA---PDMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADVE 243

Query: 246 SNGDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSKKRTVIYVKKGT 305
            +    R   R L   D+RK ADIVVAKD SGKY+TI  AL+ VP KS+KRT+IYVKKG 
Sbjct: 244 VDFHAGR---RLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKGV 303

Query: 306 YKENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAAKGKGFIAIDMGFRN 365
           Y ENV+V K  WNV++VGDG + +IV+G LNVIDGTPTF TATFA  GKGF+A DMGF N
Sbjct: 304 YFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFIN 363

Query: 366 TAGPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYGTVDFIFGNSAV 425
           TAGP+KHQAVALM ++D + FYRC M+AYQDTLY H+ RQFYREC + GTVDFIFGNSA 
Sbjct: 364 TAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSAS 423

Query: 426 VLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYENLKTTETYLGRPWKN 485
           VLQ+C I PR P+ GQ+NTITAQGR DPN NTGISI   +I P  +L    T+LGRPWKN
Sbjct: 424 VLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWKN 483

Query: 486 YSTTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWTGVKNIDI 545
           +STTV M S L   I   GWLPW G SAPDTIFY E++N GPG+ST  RVKW G++ +  
Sbjct: 484 FSTTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLST 543

Query: 546 KTAKKFTVSSFIDGKDWISKAQVPFKASI 568
           K A +FTV  FIDG  W+   +VPF++ +
Sbjct: 544 KEANRFTVKPFIDGGRWLPATKVPFRSGL 561

[Close]

BLAST of Bhi08G000856 vs. ExPASy Swiss-Prot
Match: Q9FF78 (Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3702 GN=PME46 PE=2 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 3.7e-161
Identity = 301/566 (53.18%), Postives = 395/566 (69.79%), Query Frame = 0


Query: 7   YGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVGVVVAVVFGTRNASNNNDDSNNQSG-A 66
           YG++DE +Q +L+ASR+T+KRIA+I +SS+VLV +VV  V GT    N+       +G  
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEP 63

Query: 67  LSTSIKAVCDVTLYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELSRVADYIFYHAI 126
           +S S+KA+CDVTL+   C    G   N+S   P ++ K +V++ + ELS+V D     + 
Sbjct: 64  ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGF---SN 123

Query: 127 TNSTDNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWLTSAATYQQTCID 186
               DN T  A+  C EL+ LA+D LN +++SS    LK  DDL+TWL+S  TYQ+TC+D
Sbjct: 124 GEHMDNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETCMD 183

Query: 187 GLTEVD-PALANLIANYLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSNGDWA 246
            L E + P+L     N+LKNSTE+TSN LAI+++  K+ D++  RR    E   +    A
Sbjct: 184 ALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVVVA 243

Query: 247 -RPIL---RKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSKKRTVIYVKKGTYK 306
             P++   R L   D++K+A IVVAKD SGKY+TI  AL  V  K++K T+IYVKKG Y 
Sbjct: 244 DLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGVYL 303

Query: 307 ENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAAKGKGFIAIDMGFRNTA 366
           ENV V K KWNVV+VGDG + TIV+  LN IDGTPTF TATFA  GKGF+A DMGF NTA
Sbjct: 304 ENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFINTA 363

Query: 367 GPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYGTVDFIFGNSAVVL 426
           GP KHQAVALM ++D S+FY+C MDA+QDT+Y+H+ RQFYR+C + GTVDFIFGN+AVV 
Sbjct: 364 GPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAVVF 423

Query: 427 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYENLKTTETYLGRPWKNYS 486
           Q C I PR P+ GQ+NTITAQGR DPNQNTGISI + +I+P +NL   +T+LGRPWK++S
Sbjct: 424 QKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKDFS 483

Query: 487 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWTGVK-NIDIK 546
           TTV M+S +   I+P GWLPW G +APDTIFYAE+ N GPG+ST  RVKW G+K ++  K
Sbjct: 484 TTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLTKK 543

Query: 547 TAKKFTVSSFIDGKDWISKAQVPFKA 566
            A KFTV  FIDG +W+   +VPF +
Sbjct: 544 EANKFTVKPFIDGNNWLPATKVPFNS 562

[Close]

BLAST of Bhi08G000856 vs. ExPASy Swiss-Prot
Match: Q43111 (Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 8.6e-134
Identity = 269/582 (46.22%), Postives = 371/582 (63.75%), Query Frame = 0


Query: 7   YGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVGVVVAVVFGTRNASNNNDDSNNQSGAL 66
           YGKV+E +Q   +  ++TRKR+ +I +SS+VL+ V++A V G    + N++ S +     
Sbjct: 10  YGKVNELEQQAYE--KKTRKRLIIIAVSSIVLIAVIIAAVAGVVIHNRNSESSPSSDSVP 69

Query: 67  ST------SIKAVCDVTLYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELSRVADYI 126
            T      S+KAVCD T YP +C ++   L  S+  DP  + KLS+++A+ ELS     +
Sbjct: 70  QTELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAIDELSSFPSKL 129

Query: 127 FYHAITNSTDNKTILALKNCHELLDLALDHLNISLS-----SSDITLLKAVDDLKTWLTS 186
             +A     D +   A+  C  +   ALD LN S+S     +  I    +V +++TWL++
Sbjct: 130 RANA---EQDARLQKAIDVCSSVFGDALDRLNDSISALGTVAGRIASSASVSNVETWLSA 189

Query: 187 AATYQQTCIDGLTEVDPALA----NLIANYLKNSTELTSNGLAIVS----FFSKLTDSLS 246
           A T Q TC+D + E++   A      I   ++NSTE  SN LAIV+      S+    + 
Sbjct: 190 ALTDQDTCLDAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKILGLLSRFETPIH 249

Query: 247 LRRLMSYEDHQSNGDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSK 306
            RRL+ + +     +      R L +++     D VVAKD SG++KTI  AL+ V  KS+
Sbjct: 250 HRRLLGFPEWLGAAE-----RRLLEEKNNDSTPDAVVAKDGSGQFKTIGEALKLVKKKSE 309

Query: 307 KRTVIYVKKGTYKENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAAKGK 366
           +R  +YVK+G Y EN+++ KN WNV+I GDG   T V GS N +DGTPTF TATFA KGK
Sbjct: 310 ERFSVYVKEGRYVENIDLDKNTWNVMIYGDGKDKTFVVGSRNFMDGTPTFETATFAVKGK 369

Query: 367 GFIAIDMGFRNTAGPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYG 426
           GFIA D+GF N AG +KHQAVAL S SD+S+F+RC  D +QDTLY+HSNRQFYR+C + G
Sbjct: 370 GFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDCDITG 429

Query: 427 TVDFIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYENLKT 486
           T+DFIFGN+AVV Q+C I PR PL  Q NTITAQG+ DPNQNTGI IQ S+I P+ N  T
Sbjct: 430 TIDFIFGNAAVVFQSCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSTITPFGNNLT 489

Query: 487 TETYLGRPWKNYSTTVFMQSNLGSLIHPSGWLPWV-GTSAPDTIFYAEFQNYGPGSSTAK 546
             TYLGRPWK++STTV MQS++G+L++P GW+ WV     P TIFYAE+QN GPG+  ++
Sbjct: 490 APTYLGRPWKDFSTTVIMQSDIGALLNPVGWMSWVPNVEPPTTIFYAEYQNSGPGADVSQ 549

Query: 547 RVKWTGVK-NIDIKTAKKFTVSSFIDGKDWISKAQVPFKASI 568
           RVKW G K  I  + A++FTV SFI G +W+  A V F +++
Sbjct: 550 RVKWAGYKPTITDRNAEEFTVQSFIQGPEWLPNAAVQFDSTL 581

[Close]

BLAST of Bhi08G000856 vs. ExPASy Swiss-Prot
Match: Q43867 (Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 4.7e-124
Identity = 259/574 (45.12%), Postives = 358/574 (62.37%), Query Frame = 0


Query: 7   YGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVGVVVAVVFGTRNASNNNDDSNNQSGAL 66
           YGKVDE   L L   ++TRKR+ ++ +S VVL+ V++A V  T    N N+ + +    L
Sbjct: 10  YGKVDEAQDLAL--KKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPEL 69

Query: 67  --STSIKAVCDVTLYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELSRVADYIFYHA 126
             STS+KA+C VT +P +C ++   L +S+  DP  + KLS+++ + EL  ++D      
Sbjct: 70  TPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISD--LPEK 129

Query: 127 ITNSTDNKTI-LALKNCHELLDLALDHLNISLSSSDITLLK------AVDDLKTWLTSAA 186
           ++  T+++ I  AL+ C +L++ ALD LN ++S+ D    K       ++DLKTWL++  
Sbjct: 130 LSKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATV 189

Query: 187 TYQQTCIDGLTEV--------DPALANLIANYLKNSTELTSNGLAIVSFFSKLTDSLSL- 246
           T  +TC D L E+        +  +   + + +  STE TSN LAIVS        L + 
Sbjct: 190 TDHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIP 249

Query: 247 ----RRLMSYEDHQSNGDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPN 306
               RRLMS+  HQ + D+ +   R+L Q    K  D+ VA D +G   T+  A+  VP 
Sbjct: 250 IHRRRRLMSHH-HQQSVDFEKWARRRLLQTAGLK-PDVTVAGDGTGDVLTVNEAVAKVPK 309

Query: 307 KSKKRTVIYVKKGTYKENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAA 366
           KS K  VIYVK GTY ENV + K+KWNV+I GDG   TI++GS N +DGTPT+ TATFA 
Sbjct: 310 KSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFAI 369

Query: 367 KGKGFIAIDMGFRNTAGPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECK 426
           +GKGFI  D+G  NTAG  KHQAVA  S SD S++Y+C  D +QDTLY HSNRQFYR+C 
Sbjct: 370 QGKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDCD 429

Query: 427 VYGTVDFIFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYEN 486
           V GT+DFIFG++AVV Q C I PR PL  Q NTITAQG+ DPNQ++G+SIQ  +I    N
Sbjct: 430 VTGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISANGN 489

Query: 487 LKTTETYLGRPWKNYSTTVFMQSNLGSLIHPSGWLPWV-GTSAPDTIFYAEFQNYGPGSS 546
           +    TYLGRPWK +STTV M++ +G+++ PSGW+ WV G   P +I Y E++N GPGS 
Sbjct: 490 V-IAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGSD 549

Query: 547 TAKRVKWTGVKNI-DIKTAKKFTVSSFIDGKDWI 557
             +RVKW G K +     A KFTV++ + G DWI
Sbjct: 550 VTQRVKWAGYKPVMSDAEAAKFTVATLLHGADWI 576

[Close]

BLAST of Bhi08G000856 vs. ExPASy Swiss-Prot
Match: Q9LUL8 (Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana OX=3702 GN=PME26 PE=2 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 9.5e-109
Identity = 236/512 (46.09%), Postives = 309/512 (60.35%), Query Frame = 0


Query: 67  STSIKAVCDVTLYPVTCQNAFGPL-ANSSHLDPSQISKLSVQLALGELSRVADYIFYHAI 126
           S+ ++ VC+VT YP +C ++   L  + +  DP  + +LS+Q+   EL+ +   +     
Sbjct: 456 SSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLFRLSLQVTFDELNSIVG-LPKKLA 515

Query: 127 TNSTDNKTILALKNCHELLDLALDHLNISLSSSDITL--------LKAVDDLKTWLTSAA 186
             + D     AL  C ++ DLA+D +N ++SS D  +           + DL TWL+SA 
Sbjct: 516 EETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVISGGKKNLNSSTIGDLITWLSSAV 575

Query: 187 TYQQTCIDGLTE--VDPALANLIANYLKNSTELTSNGLAIVSFF----SKLTDSLSLRRL 246
           T   TC D L E   +  +   + + + NSTE TSN LAIV+      SK    +  RRL
Sbjct: 576 TDIGTCGDTLDEDNYNSPIPQKLKSAMVNSTEFTSNSLAIVAQVLKKPSKSRIPVQGRRL 635

Query: 247 MSYEDHQSNGDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSKKRTV 306
           +   +  S  +W RP +R+L Q        + VA D SG  +T+  A+  VP K K   V
Sbjct: 636 L---NSNSFPNWVRPGVRRLLQAK-NLTPHVTVAADGSGDVRTVNEAVWRVPKKGKTMFV 695

Query: 307 IYVKKGTYKENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAAKGKGFIA 366
           IYVK GTY ENV + K+KWNV I GDG   TI++GS N++DG  TF+T+TFA +GKGF+ 
Sbjct: 696 IYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDGVRTFNTSTFATEGKGFMM 755

Query: 367 IDMGFRNTAGPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYGTVDF 426
            DMG  NTAGP KHQAVA  S SD+S++YRC  D YQDTLY+HSNRQ+YR C V GTVDF
Sbjct: 756 KDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYTHSNRQYYRNCDVTGTVDF 815

Query: 427 IFGNSAVVLQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYENLKTTETY 486
           IFG   VV Q C+I PR PL  Q NTITA+G  + NQNTGISI   +I P  N+ T  TY
Sbjct: 816 IFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGISIHQCTISPNGNV-TATTY 875

Query: 487 LGRPWKNYSTTVFMQSNLGSLIHPSGWLPWVGT--SAPDTIFYAEFQNYGPGSSTAKRVK 546
           LGRPWK +S TV MQS +GS ++P+GW+ W  T    P TIFY E++N GPGS  +KRVK
Sbjct: 876 LGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIFYREYKNSGPGSDLSKRVK 935

Query: 547 WTGVKNIDI-KTAKKFTVSSFIDGKD-WISKA 560
           W G K I     A +FTV  F+ G D WI KA
Sbjct: 936 WAGYKPISSDDEAARFTVKYFLRGDDNWIPKA 961

[Close]

BLAST of Bhi08G000856 vs. ExPASy TrEMBL
Match: A0A5A7VDG1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1290G00770 PE=3 SV=1)

HSP 1 Score: 1025.4 bits (2650), Expect = 9.1e-296
Identity = 521/567 (91.89%), Postives = 545/567 (96.12%), Query Frame = 0


Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVGVVVAVVFGTRNASNNNDDSN 60
           MS LRPYGKVDETDQLRLDASRRTR+RI VIVLSSVVLV VVVAVVFGTRNASNNNDDSN
Sbjct: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NQSGALSTSIKAVCDVTLYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELSRVADYI 120
           NQ+G+LS SIKAVCDVTLYP TCQ AFGP ANSSHLDP QISKL+VQLALGELS+VAD+I
Sbjct: 61  NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120

Query: 121 FYHAITNSTDNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWLTSAATYQ 180
           F HAITN+TD+KTILALKNCHELLDLALDHLNI+LSSSDITLLKAVDDLKTWLTSAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIDGLTEVDPALANLIANYLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSN 240
           QTCIDGL EVDPALA+L AN+LKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYED QSN
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240

Query: 241 GDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSKKRTVIYVKKGTYK 300
           GDWARPILRKLAQ DIRKQADIVVAKDSSGKYKTIT AL+AVP+KSKKRTVIYVKKGTYK
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAAKGKGFIAIDMGFRNTA 360
           E VEVVKNKWNVV+VGDGM ATIV+GSLNVIDGTPTFSTA+FAAKGKGFIAIDMGF NTA
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360

Query: 361 GPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GP+KHQAVALMSTSDQSIFYRCQM+AYQDTLY+HSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYENLKTTETYLGRPWKNYS 480
           QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQ S IQP++NLKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWTGVKNIDIKT 540
           TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYG GSST+KRVKW GVKNIDIKT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFKASI 568
           AKKFTVSSFIDGKDWISKAQVPF A++
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

[Close]

BLAST of Bhi08G000856 vs. ExPASy TrEMBL
Match: A0A1S3BDA4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103488419 PE=3 SV=1)

HSP 1 Score: 1025.4 bits (2650), Expect = 9.1e-296
Identity = 521/567 (91.89%), Postives = 545/567 (96.12%), Query Frame = 0


Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVGVVVAVVFGTRNASNNNDDSN 60
           MS LRPYGKVDETDQLRLDASRRTR+RI VIVLSSVVLV VVVAVVFGTRNASNNNDDSN
Sbjct: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NQSGALSTSIKAVCDVTLYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELSRVADYI 120
           NQ+G+LS SIKAVCDVTLYP TCQ AFGP ANSSHLDP QISKL+VQLALGELS+VAD+I
Sbjct: 61  NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120

Query: 121 FYHAITNSTDNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWLTSAATYQ 180
           F HAITN+TD+KTILALKNCHELLDLALDHLNI+LSSSDITLLKAVDDLKTWLTSAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIDGLTEVDPALANLIANYLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSN 240
           QTCIDGL EVDPALA+L AN+LKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYED QSN
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240

Query: 241 GDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSKKRTVIYVKKGTYK 300
           GDWARPILRKLAQ DIRKQADIVVAKDSSGKYKTIT AL+AVP+KSKKRTVIYVKKGTYK
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAAKGKGFIAIDMGFRNTA 360
           E VEVVKNKWNVV+VGDGM ATIV+GSLNVIDGTPTFSTA+FAAKGKGFIAIDMGF NTA
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360

Query: 361 GPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GP+KHQAVALMSTSDQSIFYRCQM+AYQDTLY+HSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYENLKTTETYLGRPWKNYS 480
           QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQ S IQP++NLKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWTGVKNIDIKT 540
           TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYG GSST+KRVKW GVKNIDIKT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFKASI 568
           AKKFTVSSFIDGKDWISKAQVPF A++
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

[Close]

BLAST of Bhi08G000856 vs. ExPASy TrEMBL
Match: A0A5D3C2L2 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold178G00410 PE=3 SV=1)

HSP 1 Score: 1023.5 bits (2645), Expect = 3.4e-295
Identity = 520/567 (91.71%), Postives = 544/567 (95.94%), Query Frame = 0


Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVGVVVAVVFGTRNASNNNDDSN 60
           MS LRPYGKVDETDQLRLDASRRTR+RI VIVLSSVVLV VVVAVVFGTRNASNNNDDSN
Sbjct: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NQSGALSTSIKAVCDVTLYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELSRVADYI 120
           NQ+G+LS SIKAVCDVTLYP TCQ AFGP ANSSHLDP QISKL+VQLALGELS+VAD+I
Sbjct: 61  NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120

Query: 121 FYHAITNSTDNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWLTSAATYQ 180
           F HAITN+TD+KTILALKNCHELLDLALDHLNI+LSSSDITLLKAVDDLKTWLTSAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIDGLTEVDPALANLIANYLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSN 240
           QTCIDGL EVDPALA+L AN+LKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYED QSN
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240

Query: 241 GDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSKKRTVIYVKKGTYK 300
           GDWARPILRKLAQ DIRKQADIVVAKDSSGKYKTIT AL+AVP+KSKKRTVIYVKKGTYK
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAAKGKGFIAIDMGFRNTA 360
           E VEVVKNKWNVV+VGDGM ATIV+GSLNVIDGTPTFSTA+FAAKGKGFIAIDMGF NTA
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360

Query: 361 GPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GP+KHQAVALMSTSDQSIFYRCQM+AYQDTLY+HSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYENLKTTETYLGRPWKNYS 480
           QNCTIAPRLPLLGQ NTITAQGRFDPNQNTGISIQ S IQP++NLKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQNNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWTGVKNIDIKT 540
           TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYG GSST+KRVKW GVKNIDIKT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFKASI 568
           AKKFTVSSFIDGKDWISKAQVPF A++
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

[Close]

BLAST of Bhi08G000856 vs. ExPASy TrEMBL
Match: A0A0A0LY08 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G538170 PE=3 SV=1)

HSP 1 Score: 1018.1 bits (2631), Expect = 1.4e-293
Identity = 514/567 (90.65%), Postives = 541/567 (95.41%), Query Frame = 0


Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVGVVVAVVFGTRNASNNNDDSN 60
           MS LRPYGKVDETDQLRLDA RRTR+RI VIVLSSVVLV VVVAVVFGTRNASNNNDDSN
Sbjct: 1   MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NQSGALSTSIKAVCDVTLYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELSRVADYI 120
           NQ+G+LSTSIKAVCDVTLYP TCQ AFGP+ANSSHLDP QISKLSVQLALGELS+VADYI
Sbjct: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120

Query: 121 FYHAITNSTDNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWLTSAATYQ 180
           F HAIT + DNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTW+TSAATYQ
Sbjct: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180

Query: 181 QTCIDGLTEVDPALANLIANYLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSN 240
           QTCID L EVDPALA+L+AN+LKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYE+HQSN
Sbjct: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240

Query: 241 GDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSKKRTVIYVKKGTYK 300
           GDWARPILRKLAQ DIRK ADIVVAKD SGKYKTIT AL AVP+KSKKRTVIYVKKG YK
Sbjct: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300

Query: 301 ENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAAKGKGFIAIDMGFRNTA 360
           ENVEVVKNKWNVV+VGDGMTATIV+G+LNV+DGTPTFSTATFAAKGKGFIAIDMGF NTA
Sbjct: 301 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360

Query: 361 GPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GP+KHQAVALMSTSDQSIFYRC+M+AYQDTLY+HSNRQFYRECKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYENLKTTETYLGRPWKNYS 480
           QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQ S IQP+ +LKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 480

Query: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWTGVKNIDIKT 540
           TTVFMQSNLG LIHP+GWLPW+GTSAPDTIFYAEFQNYGPGSST+KRVKW GVKNID KT
Sbjct: 481 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFKASI 568
           AKKFTVSSFIDGKDWISKAQVPF A++
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

[Close]

BLAST of Bhi08G000856 vs. ExPASy TrEMBL
Match: A0A6J1KL23 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111495161 PE=3 SV=1)

HSP 1 Score: 978.0 bits (2527), Expect = 1.7e-281
Identity = 494/567 (87.13%), Postives = 530/567 (93.47%), Query Frame = 0


Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVGVVVAVVFGTRNASNNNDDSN 60
           MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VIVLSSVVLVGVVVAVVFGTRN S+ ND+S+
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRN-SSKNDESD 60

Query: 61  NQSGALSTSIKAVCDVTLYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELSRVADYI 120
           N + ALSTSIKAVCD TLYP TC NAFGPLANSSHLDP QI+K SVQLAL ELSRVADY+
Sbjct: 61  NHNEALSTSIKAVCDETLYPDTCNNAFGPLANSSHLDPGQIAKFSVQLALSELSRVADYL 120

Query: 121 FYHAITNSTDNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWLTSAATYQ 180
             HAITNSTDNKTILAL+ CHELLDLA+DHLN SLSSS+IT+LKAVDDLKTWL+SAATYQ
Sbjct: 121 LEHAITNSTDNKTILALRGCHELLDLAMDHLNDSLSSSEITMLKAVDDLKTWLSSAATYQ 180

Query: 181 QTCIDGLTEVDPALANLIANYLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSN 240
           QTCIDGL EVD AL N+IANYLKNSTE+TSNGLAIVSFFSKLTDS SLRRLMSYED  SN
Sbjct: 181 QTCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSN 240

Query: 241 GDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSKKRTVIYVKKGTYK 300
           GDWARPILRKLAQ+D+RKQADIVVAKD SGKYKTIT AL+AV +KSKKRTVIYVKKG YK
Sbjct: 241 GDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALKAVRDKSKKRTVIYVKKGVYK 300

Query: 301 ENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAAKGKGFIAIDMGFRNTA 360
           ENVEVVKNKWNVV++GDGMT+T VTG LN IDGTPTFSTATFAAKGKGFIAIDMGF NTA
Sbjct: 301 ENVEVVKNKWNVVMIGDGMTSTTVTGKLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTA 360

Query: 361 GPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYGTVDFIFGNSAVVL 420
           GP+KHQAVALMSTSDQSIFYRCQM+AYQDTLY+HSNRQFYR+CKVYGTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVL 420

Query: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYENLKTTETYLGRPWKNYS 480
           QNC IAP+LPLLGQKNTITAQGRFDPNQ+TGISIQ SSIQP+ENLKTT+TYLGRPWKNYS
Sbjct: 421 QNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTQTYLGRPWKNYS 480

Query: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWTGVKNIDIKT 540
           TTVFMQ+N GSLIHP+GWLPWVGTSAPDTIFYAEFQN GPGSSTA RVKW G+KNID KT
Sbjct: 481 TTVFMQNNFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKT 540

Query: 541 AKKFTVSSFIDGKDWISKAQVPFKASI 568
           AKKFTVSSFIDGKDWISKAQVPF A++
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFNATL 566

The following BLAST results are available for this feature:

BLAST of Bhi08G000856 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10710.1	1.8e-166	54.13	root hair specific 12	[more]
AT5G04960.1	2.6e-162	53.18	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G53840.1	3.3e-125	45.12	pectin methylesterase 1	[more]
AT3G14300.1	6.8e-110	46.09	pectinesterase family protein	[more]
AT5G49180.1	1.6e-103	39.72	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

BLAST of Bhi08G000856 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SG77	2.5e-165	54.13	Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3...	[more]
Q9FF78	3.7e-161	53.18	Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3...	[more]
Q43111	8.6e-134	46.22	Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1	[more]
Q43867	4.7e-124	45.12	Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1	[more]
Q9LUL8	9.5e-109	46.09	Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Bhi08G000856 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VDG1	9.1e-296	91.89	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1290G0077...	[more]
A0A1S3BDA4	9.1e-296	91.89	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103488419 PE=3 SV=1	[more]
A0A5D3C2L2	3.4e-295	91.71	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold178G00410...	[more]
A0A0A0LY08	1.4e-293	90.65	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G538170 PE=3 SV=1	[more]
A0A6J1KL23	1.7e-281	87.13	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111495161 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 65..215 e-value: 5.1E-28 score: 109.1
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 69..215 e-value: 3.6E-23 score: 82.6
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 63..216 e-value: 5.2E-20 score: 69.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 67..219 e-value: 2.2E-29 score: 104.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 69..220
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 256..567 e-value: 1.5E-113 score: 381.3
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 261..552 e-value: 1.7E-139 score: 463.6
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 19..564
None	No IPR available	PANTHER	PTHR31707:SF272	PECTINESTERASE/PECTINESTERASE INHIBITOR 24-RELATED	coord: 19..564
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 72..221 e-value: 1.38912E-35 score: 128.71
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 405..414
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 286..305
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 259..564

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi08M000856	Bhi08M000856	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity