Bhi08G000825 (gene) Wax gourd (B227) v1

Overview


Name	Bhi08G000825
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	berberine bridge enzyme-like 18
Location	chr8: 32087457 .. 32087763 (-)
RNA-Seq Expression	Bhi08G000825
Synteny	Bhi08G000825

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGCTGAAGAAATCTGGTCTTGCTGCTGATAATGTGATAGATGCTTATTTGGTTGATGCTAATGGGAAGGTTCATGATAGAGAGTCAATGGGGGAGGATTTGTTTTGGGCCATTAGAGGTGGCGGCGGAGGAAGCTTTGGAATAGTGGTGGCGTGGAAGGTCAAGTTGGCTCTGGTTCCGGCGACGGTGACAATTTGTTCAACCAACAGGATTTTTGAAGAAGATGGAATCAACTTAATTCATGGGTGGCAATATGTGGCTAAGAGAGTGGATGAAAATCTTTTCCTTGGCATCATTTTGACTG

mRNA sequence

ATGTTGCTGAAGAAATCTGGTCTTGCTGCTGATAATGTGATAGATGCTTATTTGGTTGATGCTAATGGGAAGGTTCATGATAGAGAGTCAATGGGGGAGGATTTGTTTTGGGCCATTAGAGGTGGCGGCGGAGGAAGCTTTGGAATAGTGGTGGCGTGGAAGGTCAAGTTGGCTCTGGTTCCGGCGACGGTGACAATTTGTTCAACCAACAGGATTTTTGAAGAAGATGGAATCAACTTAATTCATGGGTGGCAATATGTGGCTAAGAGAGTGGATGAAAATCTTTTCCTTGGCATCATTTTGACTG

Coding sequence (CDS)

ATGTTGCTGAAGAAATCTGGTCTTGCTGCTGATAATGTGATAGATGCTTATTTGGTTGATGCTAATGGGAAGGTTCATGATAGAGAGTCAATGGGGGAGGATTTGTTTTGGGCCATTAGAGGTGGCGGCGGAGGAAGCTTTGGAATAGTGGTGGCGTGGAAGGTCAAGTTGGCTCTGGTTCCGGCGACGGTGACAATTTGTTCAACCAACAGGATTTTTGAAGAAGATGGAATCAACTTAATTCATGGGTGGCAATATGTGGCTAAGAGAGTGGATGAAAATCTTTTCCTTGGCATCATTTTGACTG

Protein sequence

MLLKKSGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLALVPATVTICSTNRIFEEDGINLIHGWQYVAKRVDENLFLGIILT

Homology

[Close]

BLAST of Bhi08G000825 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 132.1 bits (331), Expect = 2.5e-31
Identity = 53/101 (52.48%), Postives = 83/101 (82.18%), Query Frame = 0


Query: 2   LLKKSGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLALVP 61
           +++K GL ADNV+DA +VDANG++ DR +MGED+FWAIRGGGGGSFG+++AWK+KL  VP
Sbjct: 196 MMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVP 255

Query: 62  ATVTICSTNRIFEEDGINLIHGWQYVAKRVDENLFLGIILT 103
           ATVT+ +  +  E+DG  +++ W+ +A ++D++LF+ +I++
Sbjct: 256 ATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIIS 296

[Close]

BLAST of Bhi08G000825 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 130.2 bits (326), Expect = 9.6e-31
Identity = 55/100 (55.00%), Postives = 80/100 (80.00%), Query Frame = 0


Query: 2   LLKKSGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLALVP 61
           +++K GL ADNV+DA +VDA+GK+ +R +MGED+FWAIRGGGGGSFG+++AWK+KL  VP
Sbjct: 198 MMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVP 257

Query: 62  ATVTICSTNRIFEEDGINLIHGWQYVAKRVDENLFLGIIL 102
             VT+ +  R  E+DG  L++ WQ VA ++DE+LF+ +I+
Sbjct: 258 EIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVII 297

[Close]

BLAST of Bhi08G000825 vs. TAIR 10
Match: AT1G30740.1 (FAD-binding Berberine family protein )

HSP 1 Score: 127.9 bits (320), Expect = 4.7e-30
Identity = 54/101 (53.47%), Postives = 81/101 (80.20%), Query Frame = 0


Query: 2   LLKKSGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLALVP 61
           +++K GL  D+V+DA +VDANG++HDR+SM EDLFWAIRGGGGGSFG+V+A+KVKL  VP
Sbjct: 191 MIRKFGLTIDHVVDATIVDANGQIHDRKSMEEDLFWAIRGGGGGSFGVVLAFKVKLVTVP 250

Query: 62  ATVTICSTNRIFEEDGINLIHGWQYVAKRVDENLFLGIILT 103
            TVT+   ++  +E+ +++++ WQ+VA R D  LF+ ++L+
Sbjct: 251 KTVTVFRVDKSVDENALDMVYKWQFVAPRTDPGLFMRVLLS 291

[Close]

BLAST of Bhi08G000825 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 122.5 bits (306), Expect = 2.0e-28
Identity = 51/96 (53.12%), Postives = 76/96 (79.17%), Query Frame = 0


Query: 2   LLKKSGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLALVP 61
           LL+K GLAADN+IDA +VDA+G++ DR++MGED FWAIRGGGG SFG++++WKVKL  VP
Sbjct: 191 LLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVP 250

Query: 62  ATVTICSTNRIFEEDGINLIHGWQYVAKRVDENLFL 98
           +T+T+    +  +++ + +I  WQY A +V ++LF+
Sbjct: 251 STITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFI 286

[Close]

BLAST of Bhi08G000825 vs. TAIR 10
Match: AT5G44390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 122.5 bits (306), Expect = 2.0e-28
Identity = 56/102 (54.90%), Postives = 76/102 (74.51%), Query Frame = 0


Query: 2   LLKKSGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLALVP 61
           L++K GLAADNV+DA +VDANGK+ DR SMGEDLFWAIRGG GGSFGI+++WK+KL  VP
Sbjct: 200 LMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILSWKIKLVPVP 259

Query: 62  ATVTICSTNRIFEED-GINLIHGWQYVAKRVDENLFLGIILT 103
            T+T+ +  + FE+D    ++  WQ +A  + + LFL +  T
Sbjct: 260 ETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFT 301

[Close]

BLAST of Bhi08G000825 vs. ExPASy Swiss-Prot
Match: A0A2S1XB67 (O-acetylstemmadenine oxidase OS=Catharanthus roseus OX=4058 GN=ASO PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 2.5e-31
Identity = 59/98 (60.20%), Postives = 79/98 (80.61%), Query Frame = 0


Query: 2   LLKKSGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLALVP 61
           LL+K GLAADN+IDA +VDA G++ DRESMGEDLFWAIRGGGG SFG++V+WKVKL  VP
Sbjct: 186 LLRKYGLAADNIIDAKIVDARGRILDRESMGEDLFWAIRGGGGASFGVIVSWKVKLVKVP 245

Query: 62  ATVTICSTNRIFEEDGINLIHGWQYVAKRVDENLFLGI 100
             VT+   ++ +EE G++L+H WQY+  ++ E+LFL +
Sbjct: 246 PMVTVFILSKTYEEGGLDLLHKWQYIEHKLPEDLFLAV 283

[Close]

BLAST of Bhi08G000825 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 3.5e-30
Identity = 53/101 (52.48%), Postives = 83/101 (82.18%), Query Frame = 0


Query: 2   LLKKSGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLALVP 61
           +++K GL ADNV+DA +VDANG++ DR +MGED+FWAIRGGGGGSFG+++AWK+KL  VP
Sbjct: 196 MMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVP 255

Query: 62  ATVTICSTNRIFEEDGINLIHGWQYVAKRVDENLFLGIILT 103
           ATVT+ +  +  E+DG  +++ W+ +A ++D++LF+ +I++
Sbjct: 256 ATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIIS 296

[Close]

BLAST of Bhi08G000825 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 1.3e-29
Identity = 55/100 (55.00%), Postives = 80/100 (80.00%), Query Frame = 0


Query: 2   LLKKSGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLALVP 61
           +++K GL ADNV+DA +VDA+GK+ +R +MGED+FWAIRGGGGGSFG+++AWK+KL  VP
Sbjct: 198 MMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVP 257

Query: 62  ATVTICSTNRIFEEDGINLIHGWQYVAKRVDENLFLGIIL 102
             VT+ +  R  E+DG  L++ WQ VA ++DE+LF+ +I+
Sbjct: 258 EIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVII 297

[Close]

BLAST of Bhi08G000825 vs. ExPASy Swiss-Prot
Match: Q9SA89 (Berberine bridge enzyme-like 12 OS=Arabidopsis thaliana OX=3702 GN=At1g30740 PE=2 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 6.7e-29
Identity = 54/101 (53.47%), Postives = 81/101 (80.20%), Query Frame = 0


Query: 2   LLKKSGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLALVP 61
           +++K GL  D+V+DA +VDANG++HDR+SM EDLFWAIRGGGGGSFG+V+A+KVKL  VP
Sbjct: 191 MIRKFGLTIDHVVDATIVDANGQIHDRKSMEEDLFWAIRGGGGGSFGVVLAFKVKLVTVP 250

Query: 62  ATVTICSTNRIFEEDGINLIHGWQYVAKRVDENLFLGIILT 103
            TVT+   ++  +E+ +++++ WQ+VA R D  LF+ ++L+
Sbjct: 251 KTVTVFRVDKSVDENALDMVYKWQFVAPRTDPGLFMRVLLS 291

[Close]

BLAST of Bhi08G000825 vs. ExPASy Swiss-Prot
Match: Q9FKU9 (Berberine bridge enzyme-like 25 OS=Arabidopsis thaliana OX=3702 GN=At5g44390 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 2.8e-27
Identity = 56/102 (54.90%), Postives = 76/102 (74.51%), Query Frame = 0


Query: 2   LLKKSGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLALVP 61
           L++K GLAADNV+DA +VDANGK+ DR SMGEDLFWAIRGG GGSFGI+++WK+KL  VP
Sbjct: 200 LMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILSWKIKLVPVP 259

Query: 62  ATVTICSTNRIFEED-GINLIHGWQYVAKRVDENLFLGIILT 103
            T+T+ +  + FE+D    ++  WQ +A  + + LFL +  T
Sbjct: 260 ETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFT 301

[Close]

BLAST of Bhi08G000825 vs. ExPASy TrEMBL
Match: A0A5D3C1A1 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00110 PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.6e-41
Identity = 83/102 (81.37%), Postives = 93/102 (91.18%), Query Frame = 0


Query: 1   MLLKKSGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLALV 60
           ++L+K GLAADNVIDAYLVDANGKVHDR+SMGEDLFWAIRGGGGGSFGIVVAWK+KL  V
Sbjct: 192 LMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFGIVVAWKIKLVSV 251

Query: 61  PATVTICSTNRIFEEDGINLIHGWQYVAKRVDENLFLGIILT 103
           PATVTICSTNR  + DGI L+H WQYVA ++DENLFLGI+LT
Sbjct: 252 PATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLT 293

[Close]

BLAST of Bhi08G000825 vs. ExPASy TrEMBL
Match: A0A5A7U0V5 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold466G00830 PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.6e-41
Identity = 83/102 (81.37%), Postives = 93/102 (91.18%), Query Frame = 0


Query: 1   MLLKKSGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLALV 60
           ++L+K GLAADNVIDAYLVDANGKVHDR+SMGEDLFWAIRGGGGGSFGIVVAWK+KL  V
Sbjct: 158 LMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFGIVVAWKIKLVSV 217

Query: 61  PATVTICSTNRIFEEDGINLIHGWQYVAKRVDENLFLGIILT 103
           PATVTICSTNR  + DGI L+H WQYVA ++DENLFLGI+LT
Sbjct: 218 PATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLT 259

[Close]

BLAST of Bhi08G000825 vs. ExPASy TrEMBL
Match: A0A1S3BDZ5 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488631 PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.6e-41
Identity = 83/102 (81.37%), Postives = 93/102 (91.18%), Query Frame = 0


Query: 1   MLLKKSGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLALV 60
           ++L+K GLAADNVIDAYLVDANGKVHDR+SMGEDLFWAIRGGGGGSFGIVVAWK+KL  V
Sbjct: 192 LMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFGIVVAWKIKLVSV 251

Query: 61  PATVTICSTNRIFEEDGINLIHGWQYVAKRVDENLFLGIILT 103
           PATVTICSTNR  + DGI L+H WQYVA ++DENLFLGI+LT
Sbjct: 252 PATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLT 293

[Close]

BLAST of Bhi08G000825 vs. ExPASy TrEMBL
Match: A0A6J1KHF6 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111495284 PE=3 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 4.6e-41
Identity = 81/102 (79.41%), Postives = 93/102 (91.18%), Query Frame = 0


Query: 1   MLLKKSGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLALV 60
           ++L+K GLAADNV+DAYLVDANG+VHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKL +V
Sbjct: 189 LMLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVMV 248

Query: 61  PATVTICSTNRIFEEDGINLIHGWQYVAKRVDENLFLGIILT 103
           PA VT+CSTNR  E+D I LIH WQYVA ++D+NLFLGI+LT
Sbjct: 249 PAVVTMCSTNRDVEDDAIKLIHRWQYVANQLDDNLFLGILLT 290

[Close]

BLAST of Bhi08G000825 vs. ExPASy TrEMBL
Match: A0A6J1EK38 (berberine bridge enzyme-like 28 OS=Cucurbita moschata OX=3662 GN=LOC111434051 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 6.0e-41
Identity = 81/102 (79.41%), Postives = 93/102 (91.18%), Query Frame = 0


Query: 1   MLLKKSGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLALV 60
           ++L+K GLAADNV+DAYLVDANG+VHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKL +V
Sbjct: 54  LMLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVVV 113

Query: 61  PATVTICSTNRIFEEDGINLIHGWQYVAKRVDENLFLGIILT 103
           PA VT+CSTNR  E+D I LIH WQYVA ++D+NLFLGI+LT
Sbjct: 114 PAVVTMCSTNRDVEDDAIKLIHRWQYVANQLDDNLFLGILLT 155

The following BLAST results are available for this feature:

BLAST of Bhi08G000825 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34790.1	2.5e-31	52.48	FAD-binding Berberine family protein	[more]
AT1G30760.1	9.6e-31	55.00	FAD-binding Berberine family protein	[more]
AT1G30740.1	4.7e-30	53.47	FAD-binding Berberine family protein	[more]
AT5G44440.1	2.0e-28	53.13	FAD-binding Berberine family protein	[more]
AT5G44390.1	2.0e-28	54.90	FAD-binding Berberine family protein	[more]

BLAST of Bhi08G000825 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A2S1XB67	2.5e-31	60.20	O-acetylstemmadenine oxidase OS=Catharanthus roseus OX=4058 GN=ASO PE=1 SV=1	[more]
O64743	3.5e-30	52.48	Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV...	[more]
Q93ZA3	1.3e-29	55.00	Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=...	[more]
Q9SA89	6.7e-29	53.47	Berberine bridge enzyme-like 12 OS=Arabidopsis thaliana OX=3702 GN=At1g30740 PE=...	[more]
Q9FKU9	2.8e-27	54.90	Berberine bridge enzyme-like 25 OS=Arabidopsis thaliana OX=3702 GN=At5g44390 PE=...	[more]

BLAST of Bhi08G000825 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3C1A1	1.6e-41	81.37	Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A5A7U0V5	1.6e-41	81.37	Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3BDZ5	1.6e-41	81.37	tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC10348863...	[more]
A0A6J1KHF6	4.6e-41	79.41	berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111495284 PE=3...	[more]
A0A6J1EK38	6.0e-41	79.41	berberine bridge enzyme-like 28 OS=Cucurbita moschata OX=3662 GN=LOC111434051 PE...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016169	FAD-binding, type PCMH, subdomain 2	GENE3D	3.30.465.10		coord: 1..62 e-value: 1.6E-38 score: 135.0
None	No IPR available	GENE3D	3.40.462.20		coord: 63..100 e-value: 1.6E-38 score: 135.0
None	No IPR available	PANTHER	PTHR32448	OS08G0158400 PROTEIN	coord: 1..100
IPR036318	FAD-binding, type PCMH-like superfamily	SUPERFAMILY	56176	FAD-binding/transporter-associated domain-like	coord: 5..58

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi08M000825	Bhi08M000825	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0050660	flavin adenine dinucleotide binding