Bhi08G000824 (gene) Wax gourd (B227) v1

BLAST of Bhi08G000824 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 526.6 bits (1355), Expect = 2.4e-149
Identity = 259/535 (48.41%), Postives = 372/535 (69.53%), Query Frame = 0

Query: 4   SPLILLLSLVVILLSSSAPWVASNSSEKHENFLECL---FHHSPNISKVIYTPFNSSYSS 63
           S +++  +   +LLS   P  A+ S++   +FL+CL    + S  +SKVI+TP ++S+SS
Sbjct: 8   SSVLIFFTTSTLLLSIPHPVSANRSNQ--SSFLQCLSLQLNDSNIVSKVIHTPNDTSFSS 67

Query: 64  ILNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYV 123
           +L  +++N RFS P+ PKP+LI+TP   S +Q AV C++ +G+ IRTRSGGHD+EGLSYV
Sbjct: 68  VLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYV 127

Query: 124 AYLPFIIVDLINLSSIQIEIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIVGVG 183
            + PF+I+DL NL SI ++++  + WV++GAT+GELYY I +K+RTLAFPAG CP VGVG
Sbjct: 128 THKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVG 187

Query: 184 GHLSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV 243
           GH SGGGYG L+RK+GLAAD+VIDA +VDA G   +R  MGED FWAIRGGGG SF +V+
Sbjct: 188 GHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVL 247

Query: 244 AWKVKLVLVPATVTLCFVSKTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTRQEG 303
           +WK+ L+ VP+TVT+  V+K  E+ A  +IHRWQ+VA  + ++LFI ++L       Q  
Sbjct: 248 SWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML-------QRY 307

Query: 304 KTNPTALFFSLYLGKVDELVSILNKTFPELGLAKKDCIETSWVESALIMGTGLQPGKSLE 363
           K    A F  LYLG V  L+ ++NK FPELGL + DC E SW+ES +      + G+   
Sbjct: 308 KNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA---ELGEEPI 367

Query: 364 DLLSKASLTNGNTKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISE 423
           ++L+K +  +   K KSD++++P+P  A+  +W+RL+  + E  QL+F P+GG+MS+I++
Sbjct: 368 NVLTKRTRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIAD 427

Query: 424 SESPFSHRAGYLYKIGYVTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRD 483
            E+PF HR G +Y+I Y+ YW     DV+++++ W+  +Y  M+ FV+KSPR AYIN RD
Sbjct: 428 YETPFPHRKGNIYEIQYLNYWR---GDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRD 487

Query: 484 LDIGSNNKYGKTSYKQASIWGLKYFGNNFNRLVDVKTRVDPHNFFRHEQSIPTFS 536
           LD+G      ++ Y++   WG+KYF NNF RLV VKT VDP +FF  EQSIP F+
Sbjct: 488 LDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPPFT 527

BLAST of Bhi08G000824 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 524.6 bits (1350), Expect = 9.2e-149
Identity = 269/533 (50.47%), Postives = 364/533 (68.29%), Query Frame = 0

Query: 6   LILLLSLVVILLSSSAPWVASNSSEKHENFLECLFHHSP---NISKVIYTPFNSSYSSIL 65
           LIL+L  VV +      W +S+SS   E F +CL  +S     IS  I+   N SYSS+L
Sbjct: 5   LILVLFFVVFI------WQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVL 64

Query: 66  NFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVAY 125
              +RNLRF+T +TPKP LII  +H SH+Q A+ C K + LQ++ RSGGHD++GLSYV Y
Sbjct: 65  QANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTY 124

Query: 126 --LPFIIVDLINLSSIQIEIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIVGVG 185
              PF ++D+ NL S+ +++ + TAWV++GA LGE+YY I EKS+TLA+PAG CP VGVG
Sbjct: 125 SGKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVG 184

Query: 186 GHLSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV 245
           GH+SGGGYG +MRKYGL  DN IDA +VD NG+  DR+ MGEDL+WAI GGGGGS+G+V+
Sbjct: 185 GHISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVL 244

Query: 246 AWKVKLVLVPATVTLCFVSKTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTRQEG 305
           A+K+ LV VP  VT+  +S+T E+ AT++IHRWQ VA  L + LFI  ++   N T    
Sbjct: 245 AYKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQ 304

Query: 306 KTNPTALFFSLYLGKVDELVSILNKTFPELGLAKKDCIETSWVESALIMGTGLQPGKSLE 365
           KT  T  F +++LG    L+SILN+ FPELGL + DC ETSW++S L   T +Q G S E
Sbjct: 305 KTVRTT-FIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFW-TNIQVGSS-E 364

Query: 366 DLLSKASLTNGNTKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISE 425
            LL + +      K KSDY+++PI    +E IW+  K+ ++E+P + F PYGG M +IS 
Sbjct: 365 TLLLQRNQPVNYLKRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGGEMGRISS 424

Query: 426 SESPFSHRAGYLYKIGYVTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRD 485
           + +PF +RAG L+KI Y   W  +D  +  R++   R+LY +MTPFVSK+PR ++ NYRD
Sbjct: 425 TVTPFPYRAGNLWKIQYGANW--RDETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRD 484

Query: 486 LDIGSNNKYGK-TSYKQASIWGLKYFGNNFNRLVDVKTRVDPHNFFRHEQSIP 533
           +D+G N+  GK +SY +   +G KYF  NF RLV +KTRVD  NFFR+EQSIP
Sbjct: 485 VDLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIP 524

BLAST of Bhi08G000824 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 517.7 bits (1332), Expect = 1.1e-146
Identity = 258/532 (48.50%), Postives = 368/532 (69.17%), Query Frame = 0

Query: 10  LSLVVILLSSSAPWVASNSSEKHENFLECLFHHSP---NISKVIYTPFNSS--YSSILNF 69
           L LV +LL S    V  +SS   ++F++CL  +S     I+   ++P  ++  +   L  
Sbjct: 12  LFLVTLLLIS----VPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELES 71

Query: 70  TLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVA--Y 129
           T +NLR+ TP+ PKP+ I  P + +H+Q AV+C+K   L +R RSGGHD+EGLS+VA   
Sbjct: 72  TAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDE 131

Query: 130 LPFIIVDLINLSSIQIEIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIVGVGGH 189
            PF+IVDL  L  + +++++N+AW  +GAT+GE+YYRI EKS+T  FPAG C  +G+GGH
Sbjct: 132 TPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGH 191

Query: 190 LSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAW 249
           L GG YG +MRK+GL ADNV+DA +VDANG+  DR +MGED+FWAIRGGGGGSFG+++AW
Sbjct: 192 LVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAW 251

Query: 250 KVKLVLVPATVTLCFVSKTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTRQEGKT 309
           K+KLV VPATVT+  V+KT E+  T ++++W+ +A  LD++LFI +++S  + T + G  
Sbjct: 252 KIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNR 311

Query: 310 NPTALFFSLYLGKVDELVSILNKTFPELGLAKKDCIETSWVESALIMGTGLQPGKSLEDL 369
             +  + + +LG  + L+ ++ K+FPELGL KKDC E SW++S + +  G     + E L
Sbjct: 312 TISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYI-AGFPNSAAPEAL 371

Query: 370 LSKASLTNGNTKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESE 429
           L+  SL   + K KSD++K+PIP+  +EG+W+R   ED   P  ++ PYGG MS+ISESE
Sbjct: 372 LAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEED--SPLTIWNPYGGMMSRISESE 431

Query: 430 SPFSHRAGYLYKIGYVTYWEQQDNDV-EKRHLSWIRELYSYMTPFVSKSPRAAYINYRDL 489
            PF HR G L+KI +++ W  QD  V E+RH+ WIRE+YSYM  +VSK+PR AY+NYRDL
Sbjct: 432 IPFPHRNGTLFKIQWLSTW--QDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDL 491

Query: 490 DIGSNNKYGKTSYKQASIWGLKYFGNNFNRLVDVKTRVDPHNFFRHEQSIPT 534
           D+G+N   G+T  ++   WG KY+  NF RLV +K   DP NFFRHEQS+PT
Sbjct: 492 DLGTNE--GETDARE---WGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of Bhi08G000824 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 512.7 bits (1319), Expect = 3.6e-145
Identity = 264/533 (49.53%), Postives = 355/533 (66.60%), Query Frame = 0

Query: 9   LLSLVVILLSSSAPWVASNSSEKHENFLECL---FHHSPNISKVIYTPFN--SSYSSILN 68
           L S++V+  S       S+ +   + F+ C+    H    + K  + P    S +S +L 
Sbjct: 9   LFSILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLE 68

Query: 69  FTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVAYL 128
            T +NLRF   + PKP  I +P H SH+Q ++ICSK   + +R RSGGHD+EGLSYV+ +
Sbjct: 69  STAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQI 128

Query: 129 --PFIIVDLINLSSIQIEIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIVGVGG 188
             PFI++DL  +  + I I+ N+AWV+SGAT+GELYYRIAEKS+   FPAG C  +G+GG
Sbjct: 129 DKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGG 188

Query: 189 HLSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVA 248
           H++GG YG +MRKYGL ADNV+DA +VDANG+  DR +MGED FWAIRGG GGSFGI++A
Sbjct: 189 HITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILA 248

Query: 249 WKVKLVLVPATVTLCFVSKTWEEGATN-LIHRWQYVAHDLDENLFIGILLSGGNFTRQEG 308
           WK+KLV VP TVT+  V+KT ++   N +I +WQ VA  L E LFI +L    N     G
Sbjct: 249 WKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLF---NVAGTGG 308

Query: 309 KTNPTALFFSLYLGKVDELVSILNKTFPELGLAKKDCIETSWVES-ALIMGTGLQPGKSL 368
               T  + +L+LG    L++++ K+FPELGL  KDCIE SW+ES A I G       ++
Sbjct: 309 NKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNV 368

Query: 369 EDLLSKASLTNGNTKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQIS 428
             L  K+     + K KSD++K PIP + ++GI+++L  EDI  P +++ PYGG M++I 
Sbjct: 369 L-LQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDI--PLMIWNPYGGMMAKIP 428

Query: 429 ESESPFSHRAGYLYKIGYVTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYR 488
           ES+ PF HR G L+K+ YVT W   D     RH++WIR+LYSYMTP+VS +PR AY+NYR
Sbjct: 429 ESQIPFPHRKGVLFKVQYVTSWLDSDKR-PSRHINWIRDLYSYMTPYVSSNPREAYVNYR 488

Query: 489 DLDIGSNNKYGKTSYKQASIWGLKYFGNNFNRLVDVKTRVDPHNFFRHEQSIP 533
           DLD+G N K  KT  KQA +WG  YF NNFNRL+ +K +VDP NFFRHEQSIP
Sbjct: 489 DLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 534

BLAST of Bhi08G000824 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 508.1 bits (1307), Expect = 8.9e-144
Identity = 253/534 (47.38%), Postives = 372/534 (69.66%), Query Frame = 0

Query: 7   ILLLSLVVILLSSSAPWVASNSSEK--HENFLECLFHH-SPNISKVIYTPFNSSYSSILN 66
           +L L +  +LL+ S     S S+++  HENFL+CL H  + + S++I+T  + SY SILN
Sbjct: 7   LLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCLSHRINEDDSRIIHTSKDPSYFSILN 66

Query: 67  FTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVA-Y 126
            +++N RF    TPKP+ IITP   + +Q  + C++ +G+ IRTRSGGHD+EGLSY+A  
Sbjct: 67  SSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKS 126

Query: 127 LPFIIVDLINLSSIQIEIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIVGVGGH 186
            PF+++DL NL SI ++++  T WV+SGAT+GELYY I + S++LAFPAG  P VG+GG 
Sbjct: 127 RPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQ 186

Query: 187 LSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAW 246
             GGGYG LMRKYGL+ADNVIDA++VDANG F DR+ MGED FWAIRGGGG SF +V++W
Sbjct: 187 FGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSW 246

Query: 247 KVKLVLVPATVTLCFVSKTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTRQEGKT 306
           K++L+ VP+ VT+  V KT E+ A ++I++WQY+A  +  +LFI  +L        + +T
Sbjct: 247 KIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAML--------QKET 306

Query: 307 NPTALFFSLYLGKVDELVSILNKTFPELGLAKKDCIETSWVESALIMGTGLQPGKSLEDL 366
              A F  LYLG V +L++++   FPELGL   +C E SW+ES L        G+S+E +
Sbjct: 307 EVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVL----WFIKGESME-I 366

Query: 367 LSKASLTNGNTKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESE 426
           L+K   T+ + K K D+I++PIP  A++ +W+R +  +  + +++  P+GG+MS+I+++E
Sbjct: 367 LAKRKRTSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNE 426

Query: 427 SPFSHRAGYLYKIGYVTYWEQQDNDVE---KRHLSWIRELYSYMTPFVSKSPRAAYINYR 486
            PF HR G LY+I Y+ YW ++++  +   +++L W+  +Y +MTP+VSKSPR AY+N+R
Sbjct: 427 IPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFR 486

Query: 487 DLDIGSNNKYG-KTSYKQASIWGLKYFGNNFNRLVDVKTRVDPHNFFRHEQSIP 533
           D+D+G       KT Y++A +WG+KYF NNF+RLV VKT VDP +FF  EQSIP
Sbjct: 487 DIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of Bhi08G000824 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 1.8e-152
Identity = 263/509 (51.67%), Postives = 354/509 (69.55%), Query Frame = 0

Query: 33  ENFLECLFHHSPN---ISKVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPLLIITPSHIS 92
           ENFL+C   H PN     K++YT  +  Y SILN T++NLRF +  TPKPL+I+TPS+ S
Sbjct: 32  ENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNNS 91

Query: 93  HIQVAVICSKSYGLQIRTRSGGHDFEGLSYVAYLPFIIVDLINLSSIQIEIETNTAWVES 152
           HIQ  ++CSK  GLQIRTRSGGHD EG+SY++ +PF++VDL N+ SI+I++ + TAWVE+
Sbjct: 92  HIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVEA 151

Query: 153 GATLGELYYRIAEKSRTLAFPAGTCPIVGVGGHLSGGGYGMLMRKYGLAADNVIDAYLVD 212
           GATLGE+YY I EK+  L+FP G CP VGVGGH SGGGYG LMR YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVLVPATVTLCFVSKTWE-EGATN 272
            +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+  T+  V K  E  G   
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVK 271

Query: 273 LIHRWQYVAHDLDENLFIGILLSGGNFTRQEGKTNPT--ALFFSLYLGKVDELVSILNKT 332
           L ++WQ +A+  D++L +       N T   GK   T    F S++ G VD LV ++NK+
Sbjct: 272 LFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELGLAKKDCIETSWVESALIMGTGLQ---PGKSLEDLLSKASLTNGNTKIKSDYIKQP 392
           FPELG+ K DC E SW+++ +     +         E LL +++       IK DY+K+P
Sbjct: 332 FPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKKP 391

Query: 393 IPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESESPFSHRAGYLYKIGYVTYWEQ 452
           IP  A+  I ++L  ED+     +  PYGG M +ISES  PF HRAG +Y++ Y   WE+
Sbjct: 392 IPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWEK 451

Query: 453 QDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNKYGKTSYKQASIWGLK 512
           Q+++  ++H++W+R +Y++ TP+VS++PR AY+NYRDLD+G  N     +Y QA IWG K
Sbjct: 452 QEDN--EKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARIWGEK 511

Query: 513 YFGNNFNRLVDVKTRVDPHNFFRHEQSIP 533
           YFG NFNRLV VKT+VDP+NFFR+EQSIP
Sbjct: 512 YFGKNFNRLVKVKTKVDPNNFFRNEQSIP 538

BLAST of Bhi08G000824 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 5.1e-152
Identity = 269/520 (51.73%), Postives = 368/520 (70.77%), Query Frame = 0

Query: 24  VASNSSEKHENFLECLFHHSPNIS---KVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPL 83
           + ++ +   ENFL+C   + PN +   K++YT  N  Y S+LN T+ NLRF++  TPKPL
Sbjct: 23  IQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPL 82

Query: 84  LIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVAYLPFIIVDLINLSSIQIEI 143
           +I+TPSH+SHIQ  ++CSK  GLQIRTRSGGHD EG+SY++ +PF+IVDL N+ SI+I++
Sbjct: 83  VIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDV 142

Query: 144 ETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIVGVGGHLSGGGYGMLMRKYGLAAD 203
            + TAWVE+GATLGE+YY + EK+  L+  AG CP V  GGH  GGGYG LMR YGLAAD
Sbjct: 143 HSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAAD 202

Query: 204 NVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVLVPATVTLCFVSK 263
           N+IDA+LV+ +G+  DR+SMGEDLFWA+RGGG  SFGI+VAWK++LV VP + T+  V K
Sbjct: 203 NIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPKS-TMFSVKK 262

Query: 264 TWE-EGATNLIHRWQYVAHDLDENLFIGILLSGGNFTRQEGKTNPTAL---FFSLYLGKV 323
             E      L+++WQ +A+  D++L +       N T  +GK N TA+   F S++LG V
Sbjct: 263 IMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGK-NKTAIHTYFSSVFLGGV 322

Query: 324 DELVSILNKTFPELGLAKKDCIETSWVESALIMGTGL----QPGKSLEDLLSKASLTNGN 383
           D LV ++NK+FPELG+ K DC + SW+++ +I  +G+        + E LL +++  NG 
Sbjct: 323 DSLVDLMNKSFPELGIKKTDCRQLSWIDT-IIFYSGVVNYDTDNFNKEILLDRSAGQNGA 382

Query: 384 TKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESESPFSHRAGYL 443
            KIK DY+K+PIP +    I ++L  EDI        PYGG M +ISES  PF HRAG L
Sbjct: 383 FKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGIL 442

Query: 444 YKIGYVTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNKYGKT 503
           Y++ Y+  WE+Q+++  ++HL+WIR +Y++MTP+VSK+PR AY+NYRDLDIG N+     
Sbjct: 443 YELWYICSWEKQEDN--EKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPN 502

Query: 504 SYKQASIWGLKYFGNNFNRLVDVKTRVDPHNFFRHEQSIP 533
           +Y QA IWG KYFG NF+RLV VKT VDP+NFFR+EQSIP
Sbjct: 503 NYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIP 537

BLAST of Bhi08G000824 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 4.3e-151
Identity = 260/509 (51.08%), Postives = 359/509 (70.53%), Query Frame = 0

Query: 33  ENFLECLFHHSP-NI--SKVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPLLIITPSHIS 92
           ENFL+C   + P N+  +K++YT  +  Y SILN T++NLRF++  TPKPL+IITP ++S
Sbjct: 32  ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVS 91

Query: 93  HIQVAVICSKSYGLQIRTRSGGHDFEGLSYVAYLPFIIVDLINLSSIQIEIETNTAWVES 152
           HIQ  ++CSK  GLQIRTRSGGHD EG+SY++ +PF+IVDL N+ S++I++ + TAWVE+
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEA 151

Query: 153 GATLGELYYRIAEKSRTLAFPAGTCPIVGVGGHLSGGGYGMLMRKYGLAADNVIDAYLVD 212
           GATLGE+YY I E +  L+FPAG CP VG GGH SGGGYG LMR YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVLVPATVTLCFVSKTWE-EGATN 272
            +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  T+  V K  E      
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 273 LIHRWQYVAHDLDENLFIGILLSGGNFTRQEGKTNPT--ALFFSLYLGKVDELVSILNKT 332
           L+++WQ +A+  ++ L +       N T  +GK   T  + F S++ G VD LV ++NK+
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELGLAKKDCIETSWVESALIMGTGLQPGKSL---EDLLSKASLTNGNTKIKSDYIKQP 392
           FPELG+ K DC + SW+++ +     +    +    E LL ++        IK DY+K+P
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVKKP 391

Query: 393 IPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESESPFSHRAGYLYKIGYVTYWEQ 452
           IP  A+  I ++L  ED+ +   +F PYGG M +ISES  PF HRAG +Y+I Y+  WE+
Sbjct: 392 IPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWEK 451

Query: 453 QDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNKYGKTSYKQASIWGLK 512
           Q+++  ++H++WIR +Y++ TP+VS++PR AY+NYRDLD+G  N     +Y QA IWG K
Sbjct: 452 QEDN--EKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEK 511

Query: 513 YFGNNFNRLVDVKTRVDPHNFFRHEQSIP 533
           YFG NFNRLV VKT+VDP NFFR+EQSIP
Sbjct: 512 YFGKNFNRLVKVKTKVDPDNFFRNEQSIP 538

BLAST of Bhi08G000824 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 4.3e-151
Identity = 258/509 (50.69%), Postives = 355/509 (69.74%), Query Frame = 0

Query: 33  ENFLECLFHHSPN---ISKVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPLLIITPSHIS 92
           ENFL+C   + PN     K IYT  +  Y S+LN T++NLRF++  TPKPL+I+TPS++S
Sbjct: 32  ENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTPSNVS 91

Query: 93  HIQVAVICSKSYGLQIRTRSGGHDFEGLSYVAYLPFIIVDLINLSSIQIEIETNTAWVES 152
           HIQ +++CSK  GLQIRTRSGGHD EGLSY++ +PF IVDL N+ +++++I + TAWVE+
Sbjct: 92  HIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQTAWVEA 151

Query: 153 GATLGELYYRIAEKSRTLAFPAGTCPIVGVGGHLSGGGYGMLMRKYGLAADNVIDAYLVD 212
           GATLGE+YY I E +   +FP G CP VGVGGH SGGGYG LMR YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVLVPATVTLCFVSKTWE-EGATN 272
            +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV+VP+  T+  V K  E  G   
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEIHGLVK 271

Query: 273 LIHRWQYVAHDLDENLFIGILLSGGNFTRQEGKTNPT--ALFFSLYLGKVDELVSILNKT 332
           L ++WQ +A+  D++L +       N T   GK   T    F S++LG VD LV ++NK+
Sbjct: 272 LFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLVDLMNKS 331

Query: 333 FPELGLAKKDCIETSWVESALIMGTGLQPGKS---LEDLLSKASLTNGNTKIKSDYIKQP 392
           FPELG+ K DC E SW+++ +     +    +    E LL +++       IK DY+K+ 
Sbjct: 332 FPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDRSAGKKTAFSIKLDYVKKL 391

Query: 393 IPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESESPFSHRAGYLYKIGYVTYWEQ 452
           IP  A+  I ++L  E++ +   +  PYGG M +ISES  PF HRAG +Y++ Y   WE+
Sbjct: 392 IPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELWYTATWEK 451

Query: 453 QDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNKYGKTSYKQASIWGLK 512
           Q+++  ++H++W+R +Y++ TP+VS++PR AY+NYRDLD+G  N     +Y QA IWG K
Sbjct: 452 QEDN--EKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPESPNNYTQARIWGEK 511

Query: 513 YFGNNFNRLVDVKTRVDPHNFFRHEQSIP 533
           YFG NFNRLV VKT+ DP+NFFR+EQSIP
Sbjct: 512 YFGKNFNRLVKVKTKADPNNFFRNEQSIP 538

BLAST of Bhi08G000824 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 9.6e-151
Identity = 263/510 (51.57%), Postives = 360/510 (70.59%), Query Frame = 0

Query: 33  ENFLECLFHHSP-NI--SKVIYTPFNSSYSSILNFTLRNLRFSTPNTPKPLLIITPSHIS 92
           ENFL+CL  + P N+  +K++YT  +  Y SILN T++NLRF++  TPKPL+I TP ++S
Sbjct: 32  ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNVS 91

Query: 93  HIQVAVICSKSYGLQIRTRSGGHDFEGLSYVAYLPFIIVDLINLSSIQIEIETNTAWVES 152
           HIQ  ++CSK  GLQIRTRSGGHD EG+SY++ +PF+IVDL N+ S++I++ + TAWVES
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVES 151

Query: 153 GATLGELYYRIAEKSRTLAFPAGTCPIVGVGGHLSGGGYGMLMRKYGLAADNVIDAYLVD 212
           GATLGE+YY I E +  L+FPAG CP VG GGH SGGGYG LMR YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVLVPATVTLCFVSKTWE-EGATN 272
            +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  T+  V K  E      
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 273 LIHRWQYVAHDLDENLFIGILLSGGNFTRQEGKTNPT--ALFFSLYLGKVDELVSILNKT 332
           L+++WQ +A+  ++ L +       N T  +GK   T  + F S++ G VD LV ++NK+
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELGLAKKDCIETSWVESALIMGTGL----QPGKSLEDLLSKASLTNGNTKIKSDYIKQ 392
           FPELG+ K DC + SW+++ +I  +G+          E LL ++        IK DY+K+
Sbjct: 332 FPELGIKKTDCKQLSWIDT-IIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVKK 391

Query: 393 PIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISESESPFSHRAGYLYKIGYVTYWE 452
           PIP  A+  I ++L  ED+ +   +F PYGG M +ISES  PF HRAG  Y+I Y+  WE
Sbjct: 392 PIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASWE 451

Query: 453 QQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNKYGKTSYKQASIWGL 512
           +Q+++  ++H++WIR +Y++ TP+VS++PR AY+NYRDLD+G  N     +Y QA IWG 
Sbjct: 452 KQEDN--EKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGE 511

Query: 513 KYFGNNFNRLVDVKTRVDPHNFFRHEQSIP 533
           KYFG NFNRLV VKT+VDP NFFR+EQSIP
Sbjct: 512 KYFGKNFNRLVKVKTKVDPDNFFRNEQSIP 538

BLAST of Bhi08G000824 vs. ExPASy TrEMBL
Match: A0A6J1K2F5 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490442 PE=3 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 4.1e-245
Identity = 416/541 (76.89%), Postives = 475/541 (87.80%), Query Frame = 0

Query: 1   MKSSPLILLLSLVVILLSSSAPWVASNSSEKHENFLECLFHHS---PNISKVIYTPFNSS 60
           M SSPL L LSL +++L S + WV+SN  + H+NFL+CL H+S    +IS V+YTPFNSS
Sbjct: 6   MNSSPL-LPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSS 65

Query: 61  YSSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGL 120
           YSS+LN TLRNLRFSTP+TPKPLLIITP H+SHIQ AV+CSKS+GLQIRTRSGGHDFEGL
Sbjct: 66  YSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGL 125

Query: 121 SYVAYLPFIIVDLINLSSIQIEIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIV 180
           SYVA LPFIIVDLINL+SI I++E+ TAWV+SGATLGELYYRIA+KSRTLAFPAG CP V
Sbjct: 126 SYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAKKSRTLAFPAGICPTV 185

Query: 181 GVGGHLSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGH SGGGYGML+RKYGLAADNVIDA LVDANG+F +R+ MGEDLFWAIRGGGGGSFG
Sbjct: 186 GVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGGSFG 245

Query: 241 IVVAWKVKLVLVPATVTLCFVSKTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTR 300
           IVVAWKVKLV VPA+VTLC  +KT E+GAT LIH+WQYVAH L+ N F+GILL+GGNFTR
Sbjct: 246 IVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTR 305

Query: 301 QEGKTNPTALFFSLYLGKVDELVSILNKTFPELGLAKKDCIETSWVESALIMGTGLQPGK 360
           Q GK NP ALFFSLYLGK DEL++ILN TFPELGL+KKDC+ETSWVE+ALIMGTGLQ G 
Sbjct: 306 QGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQLGS 365

Query: 361 SLEDLLSKASLTNGNTKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQ 420
            LEDLLSKA L NG+TKIKSDYIK+PIP+AA+EGIW+RL+ EDIEMPQ +F PYGG+M Q
Sbjct: 366 KLEDLLSKAPLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQ 425

Query: 421 ISESESPFSHRAGYLYKIGYVTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYIN 480
           I E E+PFSHR  YLYKIGY+  W +Q  + EKRH+ WIRELYSYMTPFVSKSPRAAY+N
Sbjct: 426 IPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLN 485

Query: 481 YRDLDIGSNNKYGKTSYKQASIWGLKYFG-NNFNRLVDVKTRVDPHNFFRHEQSIPTFSN 538
           YRDLDIGSNNKYG+TSY+QASIWG KYFG  NF+RLV VKT+VDP++FFRHEQSIPT S+
Sbjct: 486 YRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLSS 545

BLAST of Bhi08G000824 vs. ExPASy TrEMBL
Match: A0A6J1GRE1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456783 PE=3 SV=1)

HSP 1 Score: 848.6 bits (2191), Expect = 1.5e-242
Identity = 412/541 (76.16%), Postives = 473/541 (87.43%), Query Frame = 0

Query: 1   MKSSPLILLLSLVVILLSSSAPWVASNSSEKHENFLECLFHHS---PNISKVIYTPFNSS 60
           M SSPL L LSL +++L S + WV+SN  + H+NFL+CL H+S    +IS V+YTPFNSS
Sbjct: 6   MNSSPL-LPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSS 65

Query: 61  YSSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGL 120
           YSS+LN TLRNLRFSTP+TPKPLLIITP H+SHIQ AV+CSKS+GLQIRTRSGGHDFEGL
Sbjct: 66  YSSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGL 125

Query: 121 SYVAYLPFIIVDLINLSSIQIEIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIV 180
           SYVA LPFIIVDLINL+SI I++E  TAWV+SGATLGELYY IA+KS+TLAFPAG CP V
Sbjct: 126 SYVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTV 185

Query: 181 GVGGHLSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGH SGGGYGML+RKYGLAADNVIDA LVDANG+F +R+ MGEDLFWAIRGGGGGSFG
Sbjct: 186 GVGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGGGSFG 245

Query: 241 IVVAWKVKLVLVPATVTLCFVSKTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTR 300
           IVVAWKVKLV VPA+VTLC  +KT E+GAT LIH+WQYVAH L+ N F+GILL+GGNFTR
Sbjct: 246 IVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTR 305

Query: 301 QEGKTNPTALFFSLYLGKVDELVSILNKTFPELGLAKKDCIETSWVESALIMGTGLQPGK 360
           Q GK NP ALFFSLYLGK +EL++ILN TFPELGL+KKDC+ETSWVE+ALIMGTGLQ G 
Sbjct: 306 QGGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQLGS 365

Query: 361 SLEDLLSKASLTNGNTKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQ 420
            LEDLLSKA+L NG+TKIKSDYIK+PIP+AA+EGIW+RL+ EDIEMPQ +F PYGG+M Q
Sbjct: 366 KLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQ 425

Query: 421 ISESESPFSHRAGYLYKIGYVTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYIN 480
           I E E+PFSHR   LYKIGY+  W +Q  + EKRH+ WIRELYSYMTPFVSKSPRAAY+N
Sbjct: 426 IPEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLN 485

Query: 481 YRDLDIGSNNKYGKTSYKQASIWGLKYFG-NNFNRLVDVKTRVDPHNFFRHEQSIPTFSN 538
           YRDLDIGSNNKYG+TSY+QASIWG KYFG  NF+RLV VKT+VDP++FFRHEQSIPT S+
Sbjct: 486 YRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLSS 545

BLAST of Bhi08G000824 vs. ExPASy TrEMBL
Match: A0A6J1GRG1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456798 PE=3 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 6.1e-241
Identity = 409/545 (75.05%), Postives = 474/545 (86.97%), Query Frame = 0

Query: 1   MKSSPLILLLSLVVILLSSSAPWVASNSSEKHENFLECLFHHS---PNISKVIYTPFNSS 60
           M SSPL L LSL +++L S + WV++N  + HENFL+CL H+S    +IS V+YTPFNSS
Sbjct: 6   MNSSPL-LPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTPFNSS 65

Query: 61  YSSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGL 120
           YS++LNFTLRNLRFSTP+TPKPLLIITP H+SHIQ AV+CSKS+GLQIRTRSGGHDFEGL
Sbjct: 66  YSNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGL 125

Query: 121 SYVAYLPFIIVDLINLSSIQIEIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIV 180
           SYVA LPFIIVDLINL+SI I++++ TAWV+SGATLGELYY IA+KS+TL FPAG CP V
Sbjct: 126 SYVASLPFIIVDLINLNSISIDVKSKTAWVQSGATLGELYYSIAKKSQTLTFPAGICPTV 185

Query: 181 GVGGHLSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGH SGGGYG L+RKYGLAADNVIDA LVDANG+F +R+ MGEDLFWAIRGGGGGSFG
Sbjct: 186 GVGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGGSFG 245

Query: 241 IVVAWKVKLVLVPATVTLCFVSKTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTR 300
           IVVAWKVKLV VPA+VTLC V+KT E+GAT LIH+WQYVAH L+ ++F+GILL GGNFTR
Sbjct: 246 IVVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFLGILLGGGNFTR 305

Query: 301 QEGKTNPTALFFSLYLGKVDELVSILNKTFPELGLAKKDCIETSWVESALIMGT----GL 360
           Q  K NP ALFFSLYLGK DELV+ILN TFPELGL+KKDC+ETSWVE+A+IMGT    GL
Sbjct: 306 QGDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGLQLGL 365

Query: 361 QPGKSLEDLLSKASLTNGNTKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGG 420
           QPG  LEDLLSK +    +TKIKSDY+K+PIP+AA+EGIW+RL+ +DIE+PQ +F PYGG
Sbjct: 366 QPGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFVPYGG 425

Query: 421 RMSQISESESPFSHRAGYLYKIGYVTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRA 480
           RM QISESE+PFSHR  Y+YKIGYV  W +Q  D EKRH+ WIRELYSYMTPFVSKSPRA
Sbjct: 426 RMGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSKSPRA 485

Query: 481 AYINYRDLDIGSNNKYGKTSYKQASIWGLKYFGN-NFNRLVDVKTRVDPHNFFRHEQSIP 538
           AY+NYRDLDIGSNNKYG+TSY+QASIWG KYFGN NF+RLV VKT+VDP++FFRHEQSIP
Sbjct: 486 AYLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGNENFDRLVHVKTKVDPYDFFRHEQSIP 545

BLAST of Bhi08G000824 vs. ExPASy TrEMBL
Match: A0A6J1GRE5 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456787 PE=3 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 6.1e-241
Identity = 410/541 (75.79%), Postives = 469/541 (86.69%), Query Frame = 0

Query: 1   MKSSPLILLLSLVVILLSSSAPWVASNSSEKHENFLECLFHHS---PNISKVIYTPFNSS 60
           M SSPL L LSL +++L S + WV+SN ++ HENFL+CL H+S    +IS V+YTPFNSS
Sbjct: 6   MNSSPL-LPLSLTLLVLCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTPFNSS 65

Query: 61  YSSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGL 120
           YS +LNFTLRNLRFSTP+TPKPLLIITP H+SHIQ AV+CSKS+GLQIRTRSGGHD EGL
Sbjct: 66  YSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDIEGL 125

Query: 121 SYVAYLPFIIVDLINLSSIQIEIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIV 180
           SYVA LPFIIVDLINL+SI I++E+ TAWV+SGATLGELYYRIAEKSR+LAFPAG CP V
Sbjct: 126 SYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGICPTV 185

Query: 181 GVGGHLSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHLSGGGYG+L+RKYGLAADNVIDA LVDANG+F DR+ MGEDLFWAIRGGGGGSFG
Sbjct: 186 GVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGGGSFG 245

Query: 241 IVVAWKVKLVLVPATVTLCFVSKTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTR 300
           IVVAWKV LV VPATVTLC V+KT E+GAT LIH+WQYVAH L+ NLF+GILL GGNFTR
Sbjct: 246 IVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLGILLEGGNFTR 305

Query: 301 QEGKTNPTALFFSLYLGKVDELVSILNKTFPELGLAKKDCIETSWVESALIMGTGLQPGK 360
           Q  K NP ALFFS YLGK DEL++ILN TFPEL L+KKDCIETSWVE+ALIMGTGLQPG 
Sbjct: 306 QGSKINPLALFFSFYLGKADELLTILNTTFPELDLSKKDCIETSWVETALIMGTGLQPGS 365

Query: 361 SLEDLLSKASLTNGNTKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQ 420
            LEDLLSK  L   +TK+KSDY+K+PIP+AA+EGI QRL+  DIE PQ++F PYGG+MS+
Sbjct: 366 KLEDLLSKTPLMTVSTKVKSDYVKEPIPMAAIEGIRQRLRSGDIETPQIIFIPYGGKMSE 425

Query: 421 ISESESPFSHRAGYLYKIGYVTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYIN 480
           ISE E+PFSHR  Y YKIGY T W ++  D E +HL+WIRELYSYM PFVSKSPRAAY+N
Sbjct: 426 ISELETPFSHRGEYSYKIGYTTSWVERGIDAENKHLNWIRELYSYMAPFVSKSPRAAYLN 485

Query: 481 YRDLDIGSNNKYGKTSYKQASIWGLKYFG-NNFNRLVDVKTRVDPHNFFRHEQSIPTFSN 538
           YRDLDIGSNNKYG+TSY+QA IWG KYFG  NF+RLV VKT+VDP++FFRHEQSIPTFS+
Sbjct: 486 YRDLDIGSNNKYGRTSYEQAGIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTFSS 545

BLAST of Bhi08G000824 vs. ExPASy TrEMBL
Match: A0A6J1GTA5 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456788 PE=3 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 4.4e-239
Identity = 406/541 (75.05%), Postives = 464/541 (85.77%), Query Frame = 0

Query: 1   MKSSPLILLLSLVVILLSSSAPWVASNSSEKHENFLECLFHHS---PNISKVIYTPFNSS 60
           M SSP  L  SL  ++  S + WV+SN ++ HENFL+CL H+S    +IS V+YTPFNSS
Sbjct: 6   MNSSP-FLPFSLTFLVFCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTPFNSS 65

Query: 61  YSSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGL 120
           YS +LNFTLRNLRFSTP+TPKPLLIITP H+SHIQ AV+CSKS+GLQIRTRSGGHD EGL
Sbjct: 66  YSDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDIEGL 125

Query: 121 SYVAYLPFIIVDLINLSSIQIEIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIV 180
           SYVA LPFIIVDLINL+SI I++E+ TAWV+SGATLGELYYRIAEKSR+LAFPAG CP V
Sbjct: 126 SYVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGICPTV 185

Query: 181 GVGGHLSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHLSGGGYG+L+RKYGLAADNVIDA LVDANG+F DR+ MGEDLFWAIRGGGGGSFG
Sbjct: 186 GVGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGGGSFG 245

Query: 241 IVVAWKVKLVLVPATVTLCFVSKTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTR 300
           IVVAWKV LV VPATVTLC V+KT E+GAT LIH+WQYVAH L+ NLF+ ILL GGNFTR
Sbjct: 246 IVVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLSILLEGGNFTR 305

Query: 301 QEGKTNPTALFFSLYLGKVDELVSILNKTFPELGLAKKDCIETSWVESALIMGTGLQPGK 360
           Q  K NP ALFFSLYLGK DEL++ILN TFPELGL+K DC+ETSWVE+ALIMGTGLQPG 
Sbjct: 306 QGSKINPLALFFSLYLGKADELLTILNTTFPELGLSKTDCMETSWVETALIMGTGLQPGS 365

Query: 361 SLEDLLSKASLTNGNTKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQ 420
            LEDLLSK  L    TK+KSDY+K+PIP+AA+EGIWQRL+  DIE PQ++F PYGG+MS+
Sbjct: 366 KLEDLLSKTPLMTVRTKMKSDYVKEPIPMAAIEGIWQRLRSGDIETPQIIFIPYGGKMSE 425

Query: 421 ISESESPFSHRAGYLYKIGYVTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYIN 480
           ISE E+PFSHR  Y YKIGY T W +   D E +HL+WIRELYSYM PFVSKSPRAAY+N
Sbjct: 426 ISELETPFSHRGEYSYKIGYTTNWIEGGIDAENKHLNWIRELYSYMAPFVSKSPRAAYLN 485

Query: 481 YRDLDIGSNNKYGKTSYKQASIWGLKYFG-NNFNRLVDVKTRVDPHNFFRHEQSIPTFSN 538
           YRDLDIGSNNKYG+T+Y+QASIWG KYFG  NF+RLV VKT+VDP++FFRHEQSIPT S+
Sbjct: 486 YRDLDIGSNNKYGRTNYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLSS 545