Bhi08G000498 (gene) Wax gourd (B227) v1

Overview
NameBhi08G000498
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionHaloacid dehalogenase-like hydrolase (HAD) superfamily protein
Locationchr8: 19325284 .. 19327266 (+)
RNA-Seq ExpressionBhi08G000498
SyntenyBhi08G000498
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCAATTTCTCTAACCAAAATTTCCCTATTTATACTCAACCATTTCCCCTCTTTTTTTTCATCTTTCAATCCTCTGTTTCTCCTTCCCTGTTTTCATCCATATTTTGTCCCTAATTTTCTTGCTTCTTTCTCTTCCTCTCTATCTCTCTTTCTTTCTTAGTTTTGAATATTTTTGCAAACCAAAATGGAATGCAAAAACCAGCAATCCCAATCACCAAAATATGAGTGTCTTCTCTTTGGTAAGCCCTTTGGTTTTAAAACTCAACAAATTATGAACACTAAAAACTTACACTTTTAAACATTTGTATTGTTTTGATTTGGCAGATGTTGACGATACCCTTTATCCTCTTAGTTCTGGTTTGTCAAAGCAATGCACTAAAAACATTGAAGGTACTCAATAATCAATCTCACCAATCTTTTCTTCTTCCTTTATGTTTTTTGGCTTTGAATATTCTCATTTCTTTTGTTGTTTATGACAGAGTATATGGTTCAACAACTTGGGATAGAGAAAGATGGAGTTGTAGAAATGAATCAGTTTTTGTATAGGAATTATGGGACATCCATGGCTGGCCTTAAGGTCAAATCCTTGAGTTTTGATTTTCTCCAAATTTACATAAAACCCAATTGAAATTTGTATTAAAGTGTCAAACTTTTTGCTTTCTTGTGTAGGCTATTGGTTATGAGTTTGACAATGATCACTATCATAGGTATTGCCACCCTCTTTCTCTTCTCGTTTTGGATGAAACTATCAGGAACATGTTTAGAAAGACGTTTTTAAATAGTTAAAAACACTTTAAGACGTGCGATTAATGATAAACACTACTGACAAGCATGTTTTGAATATAATCTTTCAACAAATTGATCTTTCTTTATTATTTTGTAGCTTGGTTCATGGAAGATTACCGTATAACAATCTGAAGCGTGATCCAGTTCTTAGAAATCTCTTGCTTAGTTTGCCAATTCGCAAAGTTGTAAGTACTTTCCTTTCGACTAATATATACATATATCGAGTTTTAATATGTTATCAGAGAAGAATTTTCAAACTTTTTGGTTTGGTGAAACAGATTTTCTCGAATGCGGATGAAGTTCATGTTGCCAAAGTTCTAAGCAGGCTTGGTTTGGAGGGTTGCTTTGAAACGATCATATGCTTTGAGACTCTTAACTCCTCTGATTTTGACTCTGCTTCTGATGATGGCTCTGTATCTGATTCCAAAAACTCGACGAACAGCGACTCAGACGACACGCCTCCACCGCTTCCCGTCACTCCGATTCTCTGCAAACCATCTCCACAAGCATTTGAATCTGCTCTCAAGATAGCTAACATTGACCCCAAAAGAACAGTAAGAAAATTTCTTCTCATCTTCCTAATGTAAAATTACAAAACTGAAAAAATGATGATATGATGAGATTTGAATATTGGACTTGCAGTTGTTCTTTGATGACAGCATCCGCAACATAAAGACAGGGAAATCCACTGGCCTTCACACAGTGCTGGTGAGTTCATAGATACTCATTTGATCTCTTCTGCAAAATCATGGAAATGGAAGGAAATGAATGATACAAACACATATTATCTAAGAATTGAAGAAATGGGTTTTGATTAATTTGCAGGTTGGATCATCCAATAGAGGCAATGGTGTGGATTATGCATTGGAAAGCATTCATAATATAAGGGAAGCTTTGCCAGAGCTATGGGAAGTGGATGAGAAGATGAAGAACACAAGGCTTTCTGGAAAAATTGCATTGGAGGCTTCAATTGTGATGGCATAACTAATTAGTAACATCTATTTCATTAGCCTAACATAATGGAGTTTGGATGTTATATTAATCCACCCCATTTTGTAATAAACAATTTAAAATATATAACATGTAAAGCAAAGTTTATTTCTTTCTTTCCTTTTGATATTGGGGTATGCAAATATGATTGTTTATTAGAATGCAAATGACCTAAATCAACAATCTTCCCATCAAACAACATTGAAC

mRNA sequence

CTCAATTTCTCTAACCAAAATTTCCCTATTTATACTCAACCATTTCCCCTCTTTTTTTTCATCTTTCAATCCTCTGTTTCTCCTTCCCTGTTTTCATCCATATTTTGTCCCTAATTTTCTTGCTTCTTTCTCTTCCTCTCTATCTCTCTTTCTTTCTTAGTTTTGAATATTTTTGCAAACCAAAATGGAATGCAAAAACCAGCAATCCCAATCACCAAAATATGAGTGTCTTCTCTTTGATGTTGACGATACCCTTTATCCTCTTAGTTCTGGTTTGTCAAAGCAATGCACTAAAAACATTGAAGAGTATATGGTTCAACAACTTGGGATAGAGAAAGATGGAGTTGTAGAAATGAATCAGTTTTTGTATAGGAATTATGGGACATCCATGGCTGGCCTTAAGGCTATTGGTTATGAGTTTGACAATGATCACTATCATAGCTTGGTTCATGGAAGATTACCGTATAACAATCTGAAGCGTGATCCAGTTCTTAGAAATCTCTTGCTTAGTTTGCCAATTCGCAAAGTTATTTTCTCGAATGCGGATGAAGTTCATGTTGCCAAAGTTCTAAGCAGGCTTGGTTTGGAGGGTTGCTTTGAAACGATCATATGCTTTGAGACTCTTAACTCCTCTGATTTTGACTCTGCTTCTGATGATGGCTCTGTATCTGATTCCAAAAACTCGACGAACAGCGACTCAGACGACACGCCTCCACCGCTTCCCGTCACTCCGATTCTCTGCAAACCATCTCCACAAGCATTTGAATCTGCTCTCAAGATAGCTAACATTGACCCCAAAAGAACATTGTTCTTTGATGACAGCATCCGCAACATAAAGACAGGGAAATCCACTGGCCTTCACACAGTGCTGGTTGGATCATCCAATAGAGGCAATGGTGTGGATTATGCATTGGAAAGCATTCATAATATAAGGGAAGCTTTGCCAGAGCTATGGGAAGTGGATGAGAAGATGAAGAACACAAGGCTTTCTGGAAAAATTGCATTGGAGGCTTCAATTGTGATGGCATAACTAATTAGTAACATCTATTTCATTAGCCTAACATAATGGAGTTTGGATGTTATATTAATCCACCCCATTTTGTAATAAACAATTTAAAATATATAACATGTAAAGCAAAGTTTATTTCTTTCTTTCCTTTTGATATTGGGGTATGCAAATATGATTGTTTATTAGAATGCAAATGACCTAAATCAACAATCTTCCCATCAAACAACATTGAAC

Coding sequence (CDS)

ATGGAATGCAAAAACCAGCAATCCCAATCACCAAAATATGAGTGTCTTCTCTTTGATGTTGACGATACCCTTTATCCTCTTAGTTCTGGTTTGTCAAAGCAATGCACTAAAAACATTGAAGAGTATATGGTTCAACAACTTGGGATAGAGAAAGATGGAGTTGTAGAAATGAATCAGTTTTTGTATAGGAATTATGGGACATCCATGGCTGGCCTTAAGGCTATTGGTTATGAGTTTGACAATGATCACTATCATAGCTTGGTTCATGGAAGATTACCGTATAACAATCTGAAGCGTGATCCAGTTCTTAGAAATCTCTTGCTTAGTTTGCCAATTCGCAAAGTTATTTTCTCGAATGCGGATGAAGTTCATGTTGCCAAAGTTCTAAGCAGGCTTGGTTTGGAGGGTTGCTTTGAAACGATCATATGCTTTGAGACTCTTAACTCCTCTGATTTTGACTCTGCTTCTGATGATGGCTCTGTATCTGATTCCAAAAACTCGACGAACAGCGACTCAGACGACACGCCTCCACCGCTTCCCGTCACTCCGATTCTCTGCAAACCATCTCCACAAGCATTTGAATCTGCTCTCAAGATAGCTAACATTGACCCCAAAAGAACATTGTTCTTTGATGACAGCATCCGCAACATAAAGACAGGGAAATCCACTGGCCTTCACACAGTGCTGGTTGGATCATCCAATAGAGGCAATGGTGTGGATTATGCATTGGAAAGCATTCATAATATAAGGGAAGCTTTGCCAGAGCTATGGGAAGTGGATGAGAAGATGAAGAACACAAGGCTTTCTGGAAAAATTGCATTGGAGGCTTCAATTGTGATGGCATAA

Protein sequence

MECKNQQSQSPKYECLLFDVDDTLYPLSSGLSKQCTKNIEEYMVQQLGIEKDGVVEMNQFLYRNYGTSMAGLKAIGYEFDNDHYHSLVHGRLPYNNLKRDPVLRNLLLSLPIRKVIFSNADEVHVAKVLSRLGLEGCFETIICFETLNSSDFDSASDDGSVSDSKNSTNSDSDDTPPPLPVTPILCKPSPQAFESALKIANIDPKRTLFFDDSIRNIKTGKSTGLHTVLVGSSNRGNGVDYALESIHNIREALPELWEVDEKMKNTRLSGKIALEASIVMA
Homology
BLAST of Bhi08G000498 vs. TAIR 10
Match: AT5G02230.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 336.3 bits (861), Expect = 2.4e-92
Identity = 166/280 (59.29%), Postives = 214/280 (76.43%), Query Frame = 0

Query: 1   MECKNQQ--SQSPKYECLLFDVDDTLYPLSSGLSKQCTKNIEEYMVQQLGIEKDGVVEMN 60
           ME +N+   + S KY+CLLFD+DDTLYPLSSG++++C  NI++YM ++LGI KD +VE++
Sbjct: 1   MEFENRYGLATSQKYDCLLFDLDDTLYPLSSGIARECGNNIKDYMTEKLGIPKDKIVELS 60

Query: 61  QFLYRNYGTSMAGLKAIGYEFDNDHYHSLVHGRLPYNNLKRDPVLRNLLLSLPIRKVIFS 120
             LY+NYGT+MAGL+AIGYEFD D YHS VHGRLPY+N+K D VLR+LLLSLP+RKVIF+
Sbjct: 61  DLLYKNYGTTMAGLRAIGYEFDYDEYHSFVHGRLPYDNIKPDLVLRSLLLSLPLRKVIFT 120

Query: 121 NADEVHVAKVLSRLGLEGCFETIICFETLNSSDFDSASDDGSVSDSKNSTNSDSDDTPPP 180
           NAD VH AK L +LGLE CFE IICFETLN    ++AS++  + D     N    +    
Sbjct: 121 NADRVHAAKALKKLGLEDCFEGIICFETLNLMHTNAASNNSEIFDIVGHFN--RSEPVGS 180

Query: 181 LPVTPILCKPSPQAFESALKIANIDPKRTLFFDDSIRNIKTGKSTGLHTVLVGSSNRGNG 240
           LP TP++CKPS  A E AL+IANIDP RTLFF+DS+RN++ GK  GL+TVLVG S +  G
Sbjct: 181 LPKTPVVCKPSESAIEKALEIANIDPNRTLFFEDSVRNVQAGKRVGLYTVLVGKSTKVKG 240

Query: 241 VDYALESIHNIREALPELWEVDEKMKNTRLSGKIALEASI 279
            DYALE+IHN++EA+PELWE D K  +   SGK+A+E S+
Sbjct: 241 ADYALENIHNMKEAIPELWESDRKSSDVGYSGKVAVETSV 278

BLAST of Bhi08G000498 vs. TAIR 10
Match: AT5G02230.2 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 336.3 bits (861), Expect = 2.4e-92
Identity = 166/280 (59.29%), Postives = 214/280 (76.43%), Query Frame = 0

Query: 1   MECKNQQ--SQSPKYECLLFDVDDTLYPLSSGLSKQCTKNIEEYMVQQLGIEKDGVVEMN 60
           ME +N+   + S KY+CLLFD+DDTLYPLSSG++++C  NI++YM ++LGI KD +VE++
Sbjct: 1   MEFENRYGLATSQKYDCLLFDLDDTLYPLSSGIARECGNNIKDYMTEKLGIPKDKIVELS 60

Query: 61  QFLYRNYGTSMAGLKAIGYEFDNDHYHSLVHGRLPYNNLKRDPVLRNLLLSLPIRKVIFS 120
             LY+NYGT+MAGL+AIGYEFD D YHS VHGRLPY+N+K D VLR+LLLSLP+RKVIF+
Sbjct: 61  DLLYKNYGTTMAGLRAIGYEFDYDEYHSFVHGRLPYDNIKPDLVLRSLLLSLPLRKVIFT 120

Query: 121 NADEVHVAKVLSRLGLEGCFETIICFETLNSSDFDSASDDGSVSDSKNSTNSDSDDTPPP 180
           NAD VH AK L +LGLE CFE IICFETLN    ++AS++  + D     N    +    
Sbjct: 121 NADRVHAAKALKKLGLEDCFEGIICFETLNLMHTNAASNNSEIFDIVGHFN--RSEPVGS 180

Query: 181 LPVTPILCKPSPQAFESALKIANIDPKRTLFFDDSIRNIKTGKSTGLHTVLVGSSNRGNG 240
           LP TP++CKPS  A E AL+IANIDP RTLFF+DS+RN++ GK  GL+TVLVG S +  G
Sbjct: 181 LPKTPVVCKPSESAIEKALEIANIDPNRTLFFEDSVRNVQAGKRVGLYTVLVGKSTKVKG 240

Query: 241 VDYALESIHNIREALPELWEVDEKMKNTRLSGKIALEASI 279
            DYALE+IHN++EA+PELWE D K  +   SGK+A+E S+
Sbjct: 241 ADYALENIHNMKEAIPELWESDRKSSDVGYSGKVAVETSV 278

BLAST of Bhi08G000498 vs. TAIR 10
Match: AT5G59480.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 318.9 bits (816), Expect = 4.0e-87
Identity = 164/272 (60.29%), Postives = 207/272 (76.10%), Query Frame = 0

Query: 6   QQSQSPKYECLLFDVDDTLYPLSSGLSKQCTKNIEEYMVQQLGIEKDGVVEMNQFLYRNY 65
           QQ+   KY+CLLFD+DDTLYPLSSGL+ +  KNI+EYMVQ+LGIE+D V E+   LY+ Y
Sbjct: 8   QQTSEAKYDCLLFDIDDTLYPLSSGLAMEVKKNIQEYMVQKLGIEEDKVQELCLSLYKIY 67

Query: 66  GTSMAGLKAIGYEFDNDHYHSLVHGRLPYNNLKRDPVLRNLLLSLPIRKVIFSNADEVHV 125
           GT+MAGLKA+GY+FD D +H  VHGRLPY+ LK DP+LRN++LSLPIRKV+F+NAD+ H 
Sbjct: 68  GTTMAGLKAVGYDFDYDDFHRFVHGRLPYSTLKPDPILRNIILSLPIRKVVFTNADKAHA 127

Query: 126 AKVLSRLGLEGCFETIICFETLNS-SDFDSASDDGSVSDSKNSTNSDSDDTPPPLPVTPI 185
           AK+++RLGLEGCFE II FETLN  +  +S  D  +       +   + D+   LP T +
Sbjct: 128 AKIIARLGLEGCFEKIISFETLNPITKTESPVDTKTREIFDIISYMANPDSSIELPKTSV 187

Query: 186 LCKPSPQAFESALKIANIDPKRTLFFDDSIRNIKTGKSTGLHTVLVGSSNRGNGVDYALE 245
           +CKPS  AFE   K+ANI+PK+TLFFDDSIRNI+TGK  GLHTV VG+S+R  GVD ALE
Sbjct: 188 VCKPSEGAFEQVFKMANINPKKTLFFDDSIRNIQTGKRVGLHTVWVGTSHREEGVDIALE 247

Query: 246 SIHNIREALPELWE-VDEKMKNTRLSGKIALE 276
            IHNIREALP+LW+ VD+K K  R   K+A+E
Sbjct: 248 HIHNIREALPQLWDAVDDKAKEIRTRQKVAIE 279

BLAST of Bhi08G000498 vs. TAIR 10
Match: AT5G59490.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 318.5 bits (815), Expect = 5.2e-87
Identity = 160/275 (58.18%), Postives = 203/275 (73.82%), Query Frame = 0

Query: 8   SQSPKYECLLFDVDDTLYPLSSGLSKQCTKNIEEYMVQQLGIEKDGVVEMNQFLYRNYGT 67
           S  P+YECLLFD+DDTLYPLSSGLS  C+ NI EYMV++LGI++DGVVE+NQ LY+ YGT
Sbjct: 6   SSPPRYECLLFDLDDTLYPLSSGLSDACSNNIIEYMVEKLGIDEDGVVELNQILYKKYGT 65

Query: 68  SMAGLKAIGYEFDNDHYHSLVHGRLPYNNLKRDPVLRNLLLSLPIRKVIFSNADEVHVAK 127
           SMAGLKA+GYEFDND YH  VHGRLPY NLK DPVLR+LLL LP+RK++FSN DEVHV K
Sbjct: 66  SMAGLKAVGYEFDNDEYHRYVHGRLPYENLKPDPVLRSLLLGLPLRKLVFSNGDEVHVMK 125

Query: 128 VLSRLGLEGCFETIICFETLNSSDFDSASDDGSVSDSKNSTNSDSDDTPPPLPVTPILCK 187
            L+RLG+E CFE II FETLN            +++++ S  +        LP  P++CK
Sbjct: 126 ALTRLGIEDCFERIISFETLNP----------DINEAELSCVTGH------LPENPVICK 185

Query: 188 PSPQAFESALKIANIDPKRTLFFDDSIRNIKTGKSTGLHTVLVGSSNRGNGVDYALESIH 247
           P+  AFE A  IA ++P +TLFFDDS RNI+TGK+ GLHTVLVG S + +G DYALESIH
Sbjct: 186 PTEIAFEKAFDIAQLNPHKTLFFDDSTRNIQTGKAVGLHTVLVGKSEKIDGSDYALESIH 245

Query: 248 NIREALPELWEV----DEKMKNTRLSGKIALEASI 279
           N++EA PELW      +++ +    + +I++E S+
Sbjct: 246 NMKEAFPELWSESIINNKETERIDYASQISIETSV 264

BLAST of Bhi08G000498 vs. TAIR 10
Match: AT5G59480.2 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 313.5 bits (802), Expect = 1.7e-85
Identity = 164/272 (60.29%), Postives = 206/272 (75.74%), Query Frame = 0

Query: 6   QQSQSPKYECLLFDVDDTLYPLSSGLSKQCTKNIEEYMVQQLGIEKDGVVEMNQFLYRNY 65
           QQ+   KY+CLLFD+DDTLYPLSSGL+ +  KNI+EYMVQ+LGIE+D V E+   LY+ Y
Sbjct: 8   QQTSEAKYDCLLFDIDDTLYPLSSGLAMEVKKNIQEYMVQKLGIEEDKVQELCLSLYKIY 67

Query: 66  GTSMAGLKAIGYEFDNDHYHSLVHGRLPYNNLKRDPVLRNLLLSLPIRKVIFSNADEVHV 125
           GT+MAGLKA+GY+FD D +H  VHGRLPY+ LK DP+LRN++LSLPIRKV F+NAD+ H 
Sbjct: 68  GTTMAGLKAVGYDFDYDDFHRFVHGRLPYSTLKPDPILRNIILSLPIRKV-FTNADKAHA 127

Query: 126 AKVLSRLGLEGCFETIICFETLNS-SDFDSASDDGSVSDSKNSTNSDSDDTPPPLPVTPI 185
           AK+++RLGLEGCFE II FETLN  +  +S  D  +       +   + D+   LP T +
Sbjct: 128 AKIIARLGLEGCFEKIISFETLNPITKTESPVDTKTREIFDIISYMANPDSSIELPKTSV 187

Query: 186 LCKPSPQAFESALKIANIDPKRTLFFDDSIRNIKTGKSTGLHTVLVGSSNRGNGVDYALE 245
           +CKPS  AFE   K+ANI+PK+TLFFDDSIRNI+TGK  GLHTV VG+S+R  GVD ALE
Sbjct: 188 VCKPSEGAFEQVFKMANINPKKTLFFDDSIRNIQTGKRVGLHTVWVGTSHREEGVDIALE 247

Query: 246 SIHNIREALPELWE-VDEKMKNTRLSGKIALE 276
            IHNIREALP+LW+ VD+K K  R   K+A+E
Sbjct: 248 HIHNIREALPQLWDAVDDKAKEIRTRQKVAIE 278

BLAST of Bhi08G000498 vs. ExPASy Swiss-Prot
Match: Q09893 (Uncharacterized protein C24B11.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC24B11.05 PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.3e-13
Identity = 60/213 (28.17%), Postives = 94/213 (44.13%), Query Frame = 0

Query: 16  LLFDVDDTLYPLSSGLSKQCTKNIEEYMVQQLGIEKDGVVEMNQFLYRNYGTSMAGLKAI 75
           + FD+D+ LYP S  +       I  +   +LGI  +    + +  YR+YG ++ GL  +
Sbjct: 8   IFFDLDNCLYPKSYKIHNMMAARITAFFSDKLGIPTEEAERLREVYYRHYGIAIRGL-VL 67

Query: 76  GYEFDNDHYHSLVHGRLPYNN-LKRDPVLRNLLLSL--PIRKVIFSNADEVHVAKVLSRL 135
            +E D   Y   V   LP    +K+D VLR +LL L    +  IF+NA  VH  +VL  L
Sbjct: 68  HHEIDAVDYDQRVDQSLPLEKVIKKDEVLREMLLELRKKYKCWIFTNAYIVHANRVLKYL 127

Query: 136 GLEGCFETIICFETLNSSDFDSASDDGSVSDSKNSTNSDSDDTPPPLPVTPILCKPSPQA 195
           G+E CF+  I +   N+ D                                ++ KP P+ 
Sbjct: 128 GIEDCFDG-ITYCDYNAKD--------------------------------LIAKPMPEM 186

Query: 196 FESALKIANI-DPKRTLFFDDSIRNIKTGKSTG 225
           +E  ++ A + D  + +F DDS  NI   +  G
Sbjct: 188 YERVMREAGVTDKDKCIFVDDSYGNILGAREFG 186

BLAST of Bhi08G000498 vs. ExPASy Swiss-Prot
Match: P53078 (Suppressor of disruption of TFIIS OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SDT1 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 6.6e-10
Identity = 63/238 (26.47%), Postives = 106/238 (44.54%), Query Frame = 0

Query: 9   QSPKYECLLFDVDDTLYPLSSGLSKQCTKNIEEYMVQQLGIEKDGVVEMNQFLYRNYGTS 68
           Q+P  +   FD+D+ LY  S+ +     ++I  +    L +  +    +N   Y+ YG +
Sbjct: 51  QNPNLKVFFFDIDNCLYKSSTRIHDLMQQSILRFFQTHLKLSPEDAHVLNNSYYKEYGLA 110

Query: 69  MAGLKAIGYEFDNDHYHSLVHGRLPYNN-LKRDPVLRNLLLSL----PIRKV-IFSNADE 128
           + GL  + ++ +   Y+ LV   LP  + LK D  LRN+LL L     I K+ +F+NA +
Sbjct: 111 IRGL-VMFHKVNALEYNRLVDDSLPLQDILKPDIPLRNMLLRLRQSGKIDKLWLFTNAYK 170

Query: 129 VHVAKVLSRLGLEGCFETIICFETLNSSDFDSASDDGSVSDSKNSTNSDSDDTPPPLPVT 188
            H  + L  LG+   F                   DG      + T++            
Sbjct: 171 NHAIRCLRLLGIADLF-------------------DGLTYCDYSRTDT------------ 230

Query: 189 PILCKPSPQAFESALKIANIDP-KRTLFFDDSIRNIKTGKSTGLHTVLVGSSNRGNGV 240
            ++CKP  +AFE A+K + +   +   F DDS +NI+TG   G+ T +    N  N +
Sbjct: 231 -LVCKPHVKAFEKAMKESGLARYENAYFIDDSGKNIETGIKLGMKTCIHLVENEVNEI 255

BLAST of Bhi08G000498 vs. ExPASy Swiss-Prot
Match: Q9ZVJ5 (Haloacid dehalogenase-like hydrolase domain-containing protein Sgpp OS=Arabidopsis thaliana OX=3702 GN=SGPP PE=1 SV=2)

HSP 1 Score: 45.8 bits (107), Expect = 9.2e-04
Identity = 25/79 (31.65%), Postives = 42/79 (53.16%), Query Frame = 0

Query: 187 KPSPQAFESALKIANIDPKRTLFFDDSIRNIKTGKSTGLHTVLVGSSNRGNGVDYALESI 246
           KP P  +  AL++ N+  + TL F+DSI  IK G + G+  + + + N  + +  A  + 
Sbjct: 164 KPHPGPYLKALEVLNVSKEHTLVFEDSISGIKAGVAAGMPVIGLTTGNPASLLMQAKPAF 223

Query: 247 HNIREALPELWEVDEKMKN 266
                A P+LW V E++ N
Sbjct: 224 LIENYADPKLWAVLEELDN 242

BLAST of Bhi08G000498 vs. ExPASy TrEMBL
Match: A0A0A0LXF2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G523630 PE=4 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 8.9e-143
Identity = 259/282 (91.84%), Postives = 271/282 (96.10%), Query Frame = 0

Query: 1   MECKNQQSQ-SPKYECLLFDVDDTLYPLSSGLSKQCTKNIEEYMVQQLGIEKDGVVEMNQ 60
           MECKNQQSQ SPKYECLLFDVDDTLYPLSSGLSKQCTKNIEEYMV++LGIEKDGVVEMNQ
Sbjct: 1   MECKNQQSQLSPKYECLLFDVDDTLYPLSSGLSKQCTKNIEEYMVEELGIEKDGVVEMNQ 60

Query: 61  FLYRNYGTSMAGLKAIGYEFDNDHYHSLVHGRLPYNNLKRDPVLRNLLLSLPIRKVIFSN 120
           FLYRNYGTSMAGLKAIGYEFDNDHYHSLVHGRLPYNNLKRDPVLRNLLLSLPIRKVIFSN
Sbjct: 61  FLYRNYGTSMAGLKAIGYEFDNDHYHSLVHGRLPYNNLKRDPVLRNLLLSLPIRKVIFSN 120

Query: 121 ADEVHVAKVLSRLGLEGCFETIICFETLNSSDFDSASDDGSVSDSKNSTNSDSDDTPPPL 180
           ADEVHVAKVLSRLGLEGCFE+IICFE+LNSS+FD++SDDGS SDSK STNSD DDTPPPL
Sbjct: 121 ADEVHVAKVLSRLGLEGCFESIICFESLNSSNFDTSSDDGSESDSKTSTNSDIDDTPPPL 180

Query: 181 PVTPILCKPSPQAFESALKIANIDPKRTLFFDDSIRNIKTGKSTGLHTVLVGSSNRGNGV 240
           PVTP+LCKPSPQAFESALKIANIDPK+TLFFDDSIRNIKTGKS+GL TVLVGSS RGNG+
Sbjct: 181 PVTPVLCKPSPQAFESALKIANIDPKKTLFFDDSIRNIKTGKSSGLRTVLVGSSKRGNGI 240

Query: 241 DYALESIHNIREALPELWEVDEKMKNTRLSGKIALEASIVMA 282
           DYALESIHNIREALPELWEVDEKMKN RLS  IAL+ S VMA
Sbjct: 241 DYALESIHNIREALPELWEVDEKMKNQRLSSNIALDTSAVMA 282

BLAST of Bhi08G000498 vs. ExPASy TrEMBL
Match: A0A1S4E1I6 (LOW QUALITY PROTEIN: uncharacterized protein LOC107990309 OS=Cucumis melo OX=3656 GN=LOC107990309 PE=4 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 4.9e-141
Identity = 256/282 (90.78%), Postives = 269/282 (95.39%), Query Frame = 0

Query: 1   MECKNQQSQ-SPKYECLLFDVDDTLYPLSSGLSKQCTKNIEEYMVQQLGIEKDGVVEMNQ 60
           MECKNQQ Q SPKYECLLFDVDDTLYPLSSGLSKQCTKNIEEYMV++LGIEKDGVVEMNQ
Sbjct: 1   MECKNQQPQLSPKYECLLFDVDDTLYPLSSGLSKQCTKNIEEYMVEELGIEKDGVVEMNQ 60

Query: 61  FLYRNYGTSMAGLKAIGYEFDNDHYHSLVHGRLPYNNLKRDPVLRNLLLSLPIRKVIFSN 120
           FLYR+YGTSMAGLKAIGYEFDNDHYHSLVHGRLPYNNLKRDPVLRNLLLSLPIRKVIFSN
Sbjct: 61  FLYRSYGTSMAGLKAIGYEFDNDHYHSLVHGRLPYNNLKRDPVLRNLLLSLPIRKVIFSN 120

Query: 121 ADEVHVAKVLSRLGLEGCFETIICFETLNSSDFDSASDDGSVSDSKNSTNSDSDDTPPPL 180
           ADEVHVAKVLSRLGLEGCFE IICFE+LNSS+ D++SDDGS SDSK STNSD+DDTPPPL
Sbjct: 121 ADEVHVAKVLSRLGLEGCFERIICFESLNSSNLDTSSDDGSESDSKTSTNSDTDDTPPPL 180

Query: 181 PVTPILCKPSPQAFESALKIANIDPKRTLFFDDSIRNIKTGKSTGLHTVLVGSSNRGNGV 240
           PVTPI+CKPSPQAFE ALKIAN+DPK+TLFFDDSIRNIK GKS+GLHTVLVGS NRGNGV
Sbjct: 181 PVTPIICKPSPQAFELALKIANVDPKKTLFFDDSIRNIKXGKSSGLHTVLVGSWNRGNGV 240

Query: 241 DYALESIHNIREALPELWEVDEKMKNTRLSGKIALEASIVMA 282
           DYALESIHNIREALPELWEVDEKMKN RLS KIAL+ S VMA
Sbjct: 241 DYALESIHNIREALPELWEVDEKMKNQRLSSKIALDTSFVMA 282

BLAST of Bhi08G000498 vs. ExPASy TrEMBL
Match: A0A5A7VPY6 (Haloacid dehalogenase-like hydrolase superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold352G00750 PE=4 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 2.4e-140
Identity = 254/278 (91.37%), Postives = 267/278 (96.04%), Query Frame = 0

Query: 1   MECKNQQSQ-SPKYECLLFDVDDTLYPLSSGLSKQCTKNIEEYMVQQLGIEKDGVVEMNQ 60
           MECKNQQ Q SPKYECLLFDVDDTLYPLSSGLSKQCTKNIEEYMV++LGIEKDGVVEMNQ
Sbjct: 1   MECKNQQPQLSPKYECLLFDVDDTLYPLSSGLSKQCTKNIEEYMVEELGIEKDGVVEMNQ 60

Query: 61  FLYRNYGTSMAGLKAIGYEFDNDHYHSLVHGRLPYNNLKRDPVLRNLLLSLPIRKVIFSN 120
           FLYR+YGTSMAGLKAIGYEFDNDHYHSLVHGRLPYNNLKRDPVLRNLLLSLPIRKVIFSN
Sbjct: 61  FLYRSYGTSMAGLKAIGYEFDNDHYHSLVHGRLPYNNLKRDPVLRNLLLSLPIRKVIFSN 120

Query: 121 ADEVHVAKVLSRLGLEGCFETIICFETLNSSDFDSASDDGSVSDSKNSTNSDSDDTPPPL 180
           ADEVHVAKVLSRLGLEGCFE IICFE+LNSS+ D++SDDGS SDSK STNSD+DDTPPPL
Sbjct: 121 ADEVHVAKVLSRLGLEGCFERIICFESLNSSNLDTSSDDGSESDSKTSTNSDTDDTPPPL 180

Query: 181 PVTPILCKPSPQAFESALKIANIDPKRTLFFDDSIRNIKTGKSTGLHTVLVGSSNRGNGV 240
           PVTPI+CKPSPQAFE ALKIAN+DPK+TLFFDDSIRNIKTGKS+GLHTVLVGS NRGNGV
Sbjct: 181 PVTPIICKPSPQAFELALKIANVDPKKTLFFDDSIRNIKTGKSSGLHTVLVGSWNRGNGV 240

Query: 241 DYALESIHNIREALPELWEVDEKMKNTRLSGKIALEAS 278
           DYALESIHNIREALPELWEVDEKMKN RLS KIAL+ S
Sbjct: 241 DYALESIHNIREALPELWEVDEKMKNQRLSSKIALDTS 278

BLAST of Bhi08G000498 vs. ExPASy TrEMBL
Match: A0A6J1HIG0 (suppressor of disruption of TFIIS-like OS=Cucurbita moschata OX=3662 GN=LOC111464702 PE=4 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 1.3e-133
Identity = 252/286 (88.11%), Postives = 261/286 (91.26%), Query Frame = 0

Query: 1   MECKNQQSQS---PKYECLLFDVDDTLYPLSSGLSKQCTKNIEEYMVQQLGIEKDGVVEM 60
           MECKNQQS+     KYECLLFDVDDTLYPL+SGLSKQCTKNIEEYMVQQLGIEKDGV EM
Sbjct: 1   MECKNQQSRQSRPQKYECLLFDVDDTLYPLTSGLSKQCTKNIEEYMVQQLGIEKDGVAEM 60

Query: 61  NQFLYRNYGTSMAGLKAIGYEFDNDHYHSLVHGRLPYNNLKRDPVLRNLLLSLPIRKVIF 120
           NQFLYRNYGTSMAGLKAIGYEFDNDHYHS VHGRLPY+NLKRDPVLR+LLLSLPIRKVIF
Sbjct: 61  NQFLYRNYGTSMAGLKAIGYEFDNDHYHSFVHGRLPYHNLKRDPVLRSLLLSLPIRKVIF 120

Query: 121 SNADEVHVAKVLSRLGLEGCFETIICFETLNSSDFDSASDDGSVSDSKNSTNS-DSD-DT 180
           SNAD+VHVAKVL+RLGLEGCF+ +ICFETLNS      SDDGS SDSK+ST   D D DT
Sbjct: 121 SNADDVHVAKVLNRLGLEGCFDRVICFETLNS------SDDGSESDSKSSTGDVDVDNDT 180

Query: 181 PPPLPVTPILCKPSPQAFESALKIANIDPKRTLFFDDSIRNIKTGKSTGLHTVLVGSSNR 240
           PPPLP TPILCKPSP AFESALKIANIDPKRTLFFDDSIRNIKTGKS GLHTVLVGSSNR
Sbjct: 181 PPPLPATPILCKPSPNAFESALKIANIDPKRTLFFDDSIRNIKTGKSIGLHTVLVGSSNR 240

Query: 241 GNGVDYALESIHNIREALPELWEVDEKMKNTRLSGKIALEASIVMA 282
           GNGVDYALESIHNIREALPELWEVDEKMKN RLSGKIALE SIVMA
Sbjct: 241 GNGVDYALESIHNIREALPELWEVDEKMKNRRLSGKIALETSIVMA 280

BLAST of Bhi08G000498 vs. ExPASy TrEMBL
Match: A0A5D3BGT5 (HAD-superfamily hydrolase, subfamily IA, variant 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G002070 PE=4 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 4.9e-133
Identity = 245/283 (86.57%), Postives = 261/283 (92.23%), Query Frame = 0

Query: 1   MECKN--QQSQSPKYECLLFDVDDTLYPLSSGLSKQCTKNIEEYMVQQLGIEKDGVVEMN 60
           ME KN  QQ Q  KYECLLFD+DDTLYPLSSGLSKQCT NIEEYMV++LGIEKD VVEMN
Sbjct: 1   MEFKNQEQQPQPSKYECLLFDMDDTLYPLSSGLSKQCTINIEEYMVEELGIEKDRVVEMN 60

Query: 61  QFLYRNYGTSMAGLKAIGYEFDNDHYHSLVHGRLPYNNLKRDPVLRNLLLSLPIRKVIFS 120
           QFLYRNYGTSMAGLKAIGYEFDNDHYHS VHGRLPYNNLK DP+L+N+LLSLPIRKVIFS
Sbjct: 61  QFLYRNYGTSMAGLKAIGYEFDNDHYHSFVHGRLPYNNLKCDPILKNILLSLPIRKVIFS 120

Query: 121 NADEVHVAKVLSRLGLEGCFETIICFETLNSSDFDSASDDGSVSDSKNSTNSDSDDTPPP 180
           NADEVHVAKVLSRLGLEGCFE IICFE+LNSSD D++SDDGS SDSK STN+D+DDT  P
Sbjct: 121 NADEVHVAKVLSRLGLEGCFERIICFESLNSSDLDTSSDDGSKSDSKTSTNNDTDDTLLP 180

Query: 181 LPVTPILCKPSPQAFESALKIANIDPKRTLFFDDSIRNIKTGKSTGLHTVLVGSSNRGNG 240
           LPVTPI+CKPSPQAFE ALKIAN+DPK+TLFFDDSIRNIKTGKS+GLHTVLVGS NRGNG
Sbjct: 181 LPVTPIICKPSPQAFELALKIANVDPKKTLFFDDSIRNIKTGKSSGLHTVLVGSWNRGNG 240

Query: 241 VDYALESIHNIREALPELWEVDEKMKNTRLSGKIALEASIVMA 282
           VDYALESIHNIREALPELWEVDEKMKN RLS KIAL+ S VMA
Sbjct: 241 VDYALESIHNIREALPELWEVDEKMKNQRLSSKIALDTSFVMA 283

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G02230.12.4e-9259.29Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT5G02230.22.4e-9259.29Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT5G59480.14.0e-8760.29Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT5G59490.15.2e-8758.18Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT5G59480.21.7e-8560.29Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
Match NameE-valueIdentityDescription
Q098931.3e-1328.17Uncharacterized protein C24B11.05 OS=Schizosaccharomyces pombe (strain 972 / ATC... [more]
P530786.6e-1026.47Suppressor of disruption of TFIIS OS=Saccharomyces cerevisiae (strain ATCC 20450... [more]
Q9ZVJ59.2e-0431.65Haloacid dehalogenase-like hydrolase domain-containing protein Sgpp OS=Arabidops... [more]
Match NameE-valueIdentityDescription
A0A0A0LXF28.9e-14391.84Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G523630 PE=4 SV=1[more]
A0A1S4E1I64.9e-14190.78LOW QUALITY PROTEIN: uncharacterized protein LOC107990309 OS=Cucumis melo OX=365... [more]
A0A5A7VPY62.4e-14091.37Haloacid dehalogenase-like hydrolase superfamily protein OS=Cucumis melo var. ma... [more]
A0A6J1HIG01.3e-13388.11suppressor of disruption of TFIIS-like OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A5D3BGT54.9e-13386.57HAD-superfamily hydrolase, subfamily IA, variant 3 OS=Cucumis melo var. makuwa O... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.150.450coord: 4..97
e-value: 1.2E-16
score: 62.6
NoneNo IPR availablePFAMPF13242Hydrolase_likecoord: 187..234
e-value: 2.7E-5
score: 24.2
NoneNo IPR availableSFLDSFLDS00003Haloacid_Dehalogenasecoord: 7..260
e-value: 0.0
score: 225.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 153..180
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 153..175
NoneNo IPR availablePANTHERPTHR12725:SF114SUPPRESSOR OF DISRUPTION OF TFIIS-LIKEcoord: 1..279
NoneNo IPR availablePANTHERPTHR12725HALOACID DEHALOGENASE-LIKE HYDROLASEcoord: 1..279
IPR006439HAD hydrolase, subfamily IATIGRFAMTIGR01509TIGR01509coord: 156..230
e-value: 1.7E-13
score: 49.0
IPR010237Pyrimidine 5-nucleotidaseTIGRFAMTIGR01993TIGR01993coord: 14..230
e-value: 2.0E-66
score: 221.2
IPR010237Pyrimidine 5-nucleotidaseSFLDSFLDG01132C1.5.3:_5'-Nucleotidase_Likecoord: 7..260
e-value: 0.0
score: 225.0
IPR023214HAD superfamilyGENE3D3.40.50.1000coord: 100..265
e-value: 9.7E-25
score: 89.0
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 15..234

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi08M000498Bhi08M000498mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity