Bhi08G000355 (gene) Wax gourd (B227) v1

Overview
NameBhi08G000355
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionWound-induced protein 1
Locationchr8: 14986966 .. 14987726 (-)
RNA-Seq ExpressionBhi08G000355
SyntenyBhi08G000355
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGCATACGATGTAAATTTTCTTTTAAAAAAAAAAAATTAATTTGTTTATATATATATATGCACGAGAGTGGGATGTTGGGATGCATAAAGTATTGTTGTTTAATAAATATCCGAAATTCTTGGACACGAAATAGATATGCACTCCAACGGTATATATAAAGGATCACCCATAGACCTACAAAACCTCAACACATTCTTAACTCCATCAATATTATTTCCCAGCGGAAATTATAGAACTAACCGCCCCTCCGGCAGCCGTTCGTCGGAATTACGAAACTGGCAAACTCAGAGTGGTCGTCGGAGCAGAGGGAGAGCGTTGGGAGAGTGAAGGCTCTTTACGAGGCGTTAGGTTCCAAGGACGTGGAGACGGTGCACCGCCTTCTGGCGCCGGACCTGGAGTGGTGGTTCCATGGTCCTCCGTCGCACCAATACTTGATGCGCCTCCTCACCGGCGCCTCCTCTTCCTTCGTGTTCGCACCAATCTCCGTCGTGCCATTTGGGGCCACCGTGGTGCTGGCGGAAGGCTACAACACAAAACGCGCCGTTTCATGGGTCCACGCATGGACCGTTACTGATGGGGTCATCACCCACGTGAAGGAATATCTCAACACCTCTGTTACTGTCAAGCGCTTCGCCTCCGCCGTCGACGGGGACGGGGACTCGCCGTCCACATCGCCGCCGCCTAACTGCCAGAGTGTGTGGCAGAGCAAGGTGTGGGGAAAATCGGTGGTGCCTGCTCTTGTTTTGGCCCTTTAA

mRNA sequence

ATGGGTGCATACGATCCGTTCGTCGGAATTACGAAACTGGCAAACTCAGAGTGGTCGTCGGAGCAGAGGGAGAGCGTTGGGAGAGTGAAGGCTCTTTACGAGGCGTTAGGTTCCAAGGACGTGGAGACGGTGCACCGCCTTCTGGCGCCGGACCTGGAGTGGTGGTTCCATGGTCCTCCGTCGCACCAATACTTGATGCGCCTCCTCACCGGCGCCTCCTCTTCCTTCGTGTTCGCACCAATCTCCGTCGTGCCATTTGGGGCCACCGTGGTGCTGGCGGAAGGCTACAACACAAAACGCGCCGTTTCATGGGTCCACGCATGGACCGTTACTGATGGGGTCATCACCCACGTGAAGGAATATCTCAACACCTCTGTTACTGTCAAGCGCTTCGCCTCCGCCGTCGACGGGGACGGGGACTCGCCGTCCACATCGCCGCCGCCTAACTGCCAGAGTGTGTGGCAGAGCAAGGTGTGGGGAAAATCGGTGGTGCCTGCTCTTGTTTTGGCCCTTTAA

Coding sequence (CDS)

ATGGGTGCATACGATCCGTTCGTCGGAATTACGAAACTGGCAAACTCAGAGTGGTCGTCGGAGCAGAGGGAGAGCGTTGGGAGAGTGAAGGCTCTTTACGAGGCGTTAGGTTCCAAGGACGTGGAGACGGTGCACCGCCTTCTGGCGCCGGACCTGGAGTGGTGGTTCCATGGTCCTCCGTCGCACCAATACTTGATGCGCCTCCTCACCGGCGCCTCCTCTTCCTTCGTGTTCGCACCAATCTCCGTCGTGCCATTTGGGGCCACCGTGGTGCTGGCGGAAGGCTACAACACAAAACGCGCCGTTTCATGGGTCCACGCATGGACCGTTACTGATGGGGTCATCACCCACGTGAAGGAATATCTCAACACCTCTGTTACTGTCAAGCGCTTCGCCTCCGCCGTCGACGGGGACGGGGACTCGCCGTCCACATCGCCGCCGCCTAACTGCCAGAGTGTGTGGCAGAGCAAGGTGTGGGGAAAATCGGTGGTGCCTGCTCTTGTTTTGGCCCTTTAA

Protein sequence

MGAYDPFVGITKLANSEWSSEQRESVGRVKALYEALGSKDVETVHRLLAPDLEWWFHGPPSHQYLMRLLTGASSSFVFAPISVVPFGATVVLAEGYNTKRAVSWVHAWTVTDGVITHVKEYLNTSVTVKRFASAVDGDGDSPSTSPPPNCQSVWQSKVWGKSVVPALVLAL
Homology
BLAST of Bhi08G000355 vs. TAIR 10
Match: AT3G10985.1 (senescence associated gene 20 )

HSP 1 Score: 103.2 bits (256), Expect = 2.1e-22
Identity = 57/112 (50.89%), Postives = 78/112 (69.64%), Query Frame = 0

Query: 66  MRLLTG----ASSSFVFAPISVVPFGATVVLAEGYNTKRAVSWVHAWTVTDGVITHVKEY 125
           MR+LTG    +SSSF F P+SVV FG+TV+ AEG +   ++SW+HAWTV +G+IT V+EY
Sbjct: 1   MRVLTGGVSPSSSSFEFVPLSVVSFGSTVI-AEGCDAATSISWIHAWTVANGIITQVREY 60

Query: 126 LNTSVTVKRFASAVDGDGDSPSTSPPPNCQSVWQSKVWGKS--VVPALVLAL 172
            NTS+TV R  + V G   S   +PP +C SVW+S+  G++   VP LVLA+
Sbjct: 61  SNTSLTVTRIGNVVAG-RRSAEIAPPSHCSSVWESQFSGRAGKPVPGLVLAI 110

BLAST of Bhi08G000355 vs. TAIR 10
Match: AT5G01740.1 (Nuclear transport factor 2 (NTF2) family protein )

HSP 1 Score: 99.8 bits (247), Expect = 2.3e-21
Identity = 60/157 (38.22%), Postives = 89/157 (56.69%), Query Frame = 0

Query: 22  QRESVGRVKALYEALGSKDVETVHRLLAPDLEWWFHGPPSHQYLMRLLTG---ASSSFVF 81
           + ++ G ++ LY+AL   D  TV +++A D+EWWFHGP   Q++MRLLTG      SF F
Sbjct: 15  EMKNQGTIELLYKALQQGDTSTVTKVVASDVEWWFHGPHDCQHMMRLLTGEPPRQVSFRF 74

Query: 82  AP--ISVVPFGATVVLAEGYNTKRAVSWVHAWTVTDGVITHVKEYLNTSVTVKRFASAVD 141
            P  + VV  G   V+AEG+   R V WVH W + DGV+T ++EY NT +TV  ++    
Sbjct: 75  EPSSVQVVVPGHDCVIAEGWEGSR-VYWVHVWKLKDGVVTELREYFNTWLTVTDYSLGAV 134

Query: 142 GDGDSPSTSPPPNCQSVWQS--KVWGKSVVPALVLAL 172
           G      T        VW+S  +   +  +P+L+LA+
Sbjct: 135 GWDMGRCT--------VWESVPRDLARGSLPSLLLAI 162

BLAST of Bhi08G000355 vs. ExPASy Swiss-Prot
Match: Q94AK6 (Senescence associated gene 20 OS=Arabidopsis thaliana OX=3702 GN=SAG20 PE=2 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 3.0e-21
Identity = 57/112 (50.89%), Postives = 78/112 (69.64%), Query Frame = 0

Query: 66  MRLLTG----ASSSFVFAPISVVPFGATVVLAEGYNTKRAVSWVHAWTVTDGVITHVKEY 125
           MR+LTG    +SSSF F P+SVV FG+TV+ AEG +   ++SW+HAWTV +G+IT V+EY
Sbjct: 1   MRVLTGGVSPSSSSFEFVPLSVVSFGSTVI-AEGCDAATSISWIHAWTVANGIITQVREY 60

Query: 126 LNTSVTVKRFASAVDGDGDSPSTSPPPNCQSVWQSKVWGKS--VVPALVLAL 172
            NTS+TV R  + V G   S   +PP +C SVW+S+  G++   VP LVLA+
Sbjct: 61  SNTSLTVTRIGNVVAG-RRSAEIAPPSHCSSVWESQFSGRAGKPVPGLVLAI 110

BLAST of Bhi08G000355 vs. ExPASy Swiss-Prot
Match: P20144 (Wound-induced protein 1 OS=Solanum tuberosum OX=4113 GN=WUN1 PE=2 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 1.9e-15
Identity = 56/113 (49.56%), Postives = 73/113 (64.60%), Query Frame = 0

Query: 66  MRLLTGA----SSSFVFAPISVVPFGATVVLAEGYNTKRAVSWVHAWTVTDGVITHVKEY 125
           M++LTG     ++SF F   ++  FG +VVL EG +  R+++WVHAWTVTDGVIT V+EY
Sbjct: 1   MQILTGTAKFDNASFQFLHKTIDVFG-SVVLVEGCDPTRSITWVHAWTVTDGVITQVREY 60

Query: 126 LNTSVTVKRFASAVDGDGDSPSTSPPPNCQSVWQSKV---WGKSVVPALVLAL 172
            NTS+TV RF     G  D  S +   +C SVW+S +    GKS VP LVLAL
Sbjct: 61  FNTSLTVTRF-----GKSDISSIT-TLHCPSVWESSLPNRVGKS-VPGLVLAL 105

BLAST of Bhi08G000355 vs. ExPASy TrEMBL
Match: A0A5A7UY51 (Putative Senescence associated protein 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00340 PE=4 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 1.3e-75
Identity = 142/166 (85.54%), Postives = 154/166 (92.77%), Query Frame = 0

Query: 6   PFVGITKLANSEWSSEQRESVGRVKALYEALGSKDVETVHRLLAPDLEWWFHGPPSHQYL 65
           PFV ITKLAN EWS EQRESVGRV+ALYEALG+KDVETVHRLLAPDLEWWFHGPPSHQYL
Sbjct: 19  PFVAITKLANPEWSPEQRESVGRVRALYEALGTKDVETVHRLLAPDLEWWFHGPPSHQYL 78

Query: 66  MRLLTGASSSFVFAPISVVPFGATVVLAEGYNTKRAVSWVHAWTVTDGVITHVKEYLNTS 125
           MRLLTGASSSFVFAPIS+VP G T+VLAEG++ KRAVSWVHAWT+TDGVITHVKEYLNTS
Sbjct: 79  MRLLTGASSSFVFAPISLVPLGPTIVLAEGFHPKRAVSWVHAWTITDGVITHVKEYLNTS 138

Query: 126 VTVKRFASAVDGDGDSPSTSPPPNCQSVWQSKVWGKSVVPALVLAL 172
           VTVKRF+SA   DGDSPS SPPPNC+S+WQSKVW +S +PALVLAL
Sbjct: 139 VTVKRFSSAAT-DGDSPSISPPPNCESLWQSKVWRES-MPALVLAL 182

BLAST of Bhi08G000355 vs. ExPASy TrEMBL
Match: A0A061GYQ0 (Senescence associated gene 20, putative OS=Theobroma cacao OX=3641 GN=TCM_042245 PE=4 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 2.6e-44
Identity = 94/162 (58.02%), Postives = 118/162 (72.84%), Query Frame = 0

Query: 14  ANSEWSSEQRESVGRVKALYEALGSKDVETVHRLLAPDLEWWFHGPPSHQYLMRLLTGAS 73
           A  E   E+  +   V+ALY+AL S+DVETVHRLLA DLEWWFHGPPSHQ+LMRLLTG++
Sbjct: 83  ARLEGQEEEYSNERVVRALYDALNSRDVETVHRLLAADLEWWFHGPPSHQHLMRLLTGST 142

Query: 74  SSFVFAPISVVPFGATVVLAEGYNTKRAVSWVHAWTVTDGVITHVKEYLNTSVTVKRFAS 133
             F+F P+ V  FG ++V+ EGYN +R VSWVHAWTVT+G+IT V+EY NTS+TV R ++
Sbjct: 143 EPFIFVPLRVAAFG-SLVIVEGYNKERDVSWVHAWTVTNGIITQVREYFNTSLTVARLSN 202

Query: 134 AVDGDGDSPSTSPPP----NCQSVWQSKVWGKSVVPALVLAL 172
           +   +G  PS S  P    NCQS+WQSK+     VP LVLAL
Sbjct: 203 S---EGGPPSISSAPTVLANCQSIWQSKLCDDKAVPGLVLAL 240

BLAST of Bhi08G000355 vs. ExPASy TrEMBL
Match: A0A1U7ZU64 (uncharacterized protein LOC104597350 OS=Nelumbo nucifera OX=4432 GN=LOC104597350 PE=4 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.7e-43
Identity = 98/167 (58.68%), Postives = 121/167 (72.46%), Query Frame = 0

Query: 10  ITKLANSEWSSEQRESVGRVKALYEALGSKDVETVHRLLAPDLEWWFHGPPSHQYLMRLL 69
           + +LA S+ S E+  S+  V+ALY+ALG +DVET+ RLLAPD+EWWFHGPPSHQY+MRLL
Sbjct: 81  LPELATSQASLEEESSIRVVRALYDALGVRDVETIQRLLAPDIEWWFHGPPSHQYMMRLL 140

Query: 70  TGASS--SFVFAPISVVPFGATVVLAEGYNTKRAVSWVHAWTVTDGVITHVKEYLNTSVT 129
           TG SS  SFVF P+S   FG+T VL EG +  R+VSWVHAWTV DG+IT V+EY +TS+T
Sbjct: 141 TGVSSDESFVFVPLSFAAFGST-VLVEGCDQNRSVSWVHAWTVADGIITQVREYFDTSLT 200

Query: 130 VKRFASAVDGDGDSPSTSPPPNCQSVWQS---KVWGKSVVPALVLAL 172
           V RF  +    G SPS+S    C  +WQS   K  GKS VP LVLA+
Sbjct: 201 VSRFGKS----GPSPSSSSSIRCPHLWQSQFTKATGKS-VPGLVLAI 241

BLAST of Bhi08G000355 vs. ExPASy TrEMBL
Match: A0A2N9EI45 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS2202 PE=4 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 8.3e-43
Identity = 102/172 (59.30%), Postives = 122/172 (70.93%), Query Frame = 0

Query: 12  KLANSEWSSEQRESVGR-VKALYEALGSKDVETVHRLLAPDLEWWFHGPPSHQYLMRLLT 71
           +LANS+ S E++E   R V ALY+AL SKD + V RLLAPDLEWWFHGPP+HQ+LMRLLT
Sbjct: 5   QLANSQASLEEQEPNERVVTALYKALSSKDFDEVQRLLAPDLEWWFHGPPNHQHLMRLLT 64

Query: 72  GAS--SSFVFAPISVVPFGATVVLAEGYNTKRAVSWVHAWTVTDGVITHVKEYLNTSVTV 131
           G+S   SFVF P S+V FG+T VL EGY+ +R+VSWVHAWTVTDG+IT V+EY NTSV V
Sbjct: 65  GSSKEDSFVFVPQSIVAFGST-VLVEGYDKERSVSWVHAWTVTDGIITQVREYFNTSVIV 124

Query: 132 KRFA-SAVDGDGDSPSTSPPP--------NCQSVWQSKVWGKSVVPALVLAL 172
            RF  S   G G +  T   P         CQSVW+SK+   + VP LVLAL
Sbjct: 125 ARFGDSKGQGVGGASQTISSPAVTLKSVNKCQSVWESKLCDNNSVPGLVLAL 175

BLAST of Bhi08G000355 vs. ExPASy TrEMBL
Match: A0A438KNE8 (Wound-induced protein 1 OS=Vitis vinifera OX=29760 GN=WUN1 PE=4 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 1.8e-42
Identity = 100/172 (58.14%), Postives = 127/172 (73.84%), Query Frame = 0

Query: 9   GITKLANSEWSSEQRESVGRVKALYEALGSKDVETVHRLLAPDLEWWFHGPPSHQYLMRL 68
           G+++LA++E + E+  S   V ALYEAL ++DVETVH+LLAPDLEWWFHGPP+HQ+LMRL
Sbjct: 59  GVSELAHTEAALEE-SSKRVVGALYEALSTRDVETVHQLLAPDLEWWFHGPPNHQHLMRL 118

Query: 69  LTGASSS---FVFAPISVVPFGATVVLAEGYNTKRAVSWVHAWTVTDGVITHVKEYLNTS 128
           LTG+SSS   F F P+S+V FG+T VL EGY  +R+V+WVHAW VTDG+IT V+EY NTS
Sbjct: 119 LTGSSSSDDPFHFDPVSIVAFGST-VLVEGYEERRSVAWVHAWVVTDGIITQVREYFNTS 178

Query: 129 VTVKRFAS---AVDGDGDSPSTSPPPNCQSVWQSKV---WGKSVVPALVLAL 172
           VTV R ++   A +G   S + +    C  VWQSKV    GKS VP L+LAL
Sbjct: 179 VTVTRLSNTDKAAEGPSTSTTAARSVPCTCVWQSKVCESMGKS-VPGLLLAL 227

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G10985.12.1e-2250.89senescence associated gene 20 [more]
AT5G01740.12.3e-2138.22Nuclear transport factor 2 (NTF2) family protein [more]
Match NameE-valueIdentityDescription
Q94AK63.0e-2150.89Senescence associated gene 20 OS=Arabidopsis thaliana OX=3702 GN=SAG20 PE=2 SV=1[more]
P201441.9e-1549.56Wound-induced protein 1 OS=Solanum tuberosum OX=4113 GN=WUN1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7UY511.3e-7585.54Putative Senescence associated protein 20 OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A061GYQ02.6e-4458.02Senescence associated gene 20, putative OS=Theobroma cacao OX=3641 GN=TCM_042245... [more]
A0A1U7ZU641.7e-4358.68uncharacterized protein LOC104597350 OS=Nelumbo nucifera OX=4432 GN=LOC104597350... [more]
A0A2N9EI458.3e-4359.30Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS2202 PE=4 SV=1[more]
A0A438KNE81.8e-4258.14Wound-induced protein 1 OS=Vitis vinifera OX=29760 GN=WUN1 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.10.450.50coord: 12..136
e-value: 5.5E-12
score: 47.6
NoneNo IPR availablePANTHERPTHR33703:SF19WOUND-INDUCED PROTEIN, WUN1-RELATEDcoord: 21..171
IPR009798Wound-induced protein Wun1-likePFAMPF07107WI12coord: 66..171
e-value: 5.0E-29
score: 100.6
IPR009798Wound-induced protein Wun1-likePANTHERPTHR33703OS07G0691300 PROTEINcoord: 21..171
IPR032710NTF2-like domain superfamilySUPERFAMILY54427NTF2-likecoord: 27..129

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi08M000355Bhi08M000355mRNA