Bhi08G000301 (gene) Wax gourd (B227) v1

Overview
NameBhi08G000301
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionMitogen-activated protein kinase
Locationchr8: 12443077 .. 12446426 (+)
RNA-Seq ExpressionBhi08G000301
SyntenyBhi08G000301
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCAAATTTCAAAGTTTCAAAAGATGAAAGACGCGGAAAGTAAAATTCCAGATGAGATGGCGACGCTTTTTCTCCTTCTTCTTCCATCAATTCCAAACCAAACCCTTCTTTTTAACGCTCTCTCTCTTTCTCTCTTTCGCCATTATCACTGAAAACCTTCACTTCTTCCTTCTTCACCCTTTTTCAGACCCAACAACAAGAAAATGGAGAATGATTCTTCTTCTTCCGCTATGGATATCAAAGGCATTCCCACTTACGACTCCAAATACTTACTCTACAATGTTCTCGGCAACTTCTTTGAGATCTCTGCCAAGTACTCCCCTTCAATTCAACCTGTTGGACGCGGTGCTTACGGCATTGTTTGGTAATTATCTGTTCTGCTGCTGATTTTTGGGGTTTCAGTTCTTGTTATAAGTATTTTGATTTGGGCTTTCTTTGATTTTTTGATAATATGTCTAGATTCCCTTGTTGAATGGTTATTTCAATTATGCCCATTTTCTCTATCTTGATGCTTGCTTTGTTATCTGCTAAGATTTTTATGATTTGCTTGTTCAGTTGTACGACCAATTCTGAGACGAAAGAAGAGGTAGCGATAAAGAAGATTGGGAATGCTTTTGATAATAGGATTGATGCCAAGAGGACGCTTCGTGAGATTAAACTCCTATGCCATATGGATCATGACAATGTATTTGTCTAAAATGCTTCTCTGTTACATGCTCACCTTGATTTGTTTTGTTTAAATTTCTTCATTTCGATGTAGATTTTGCAATACTTAATATCCATTTATTGTTATGATTAAATTTCTTAATGACTGCTGGATATTTGTGTAGAATTTGAGTTGAACTAATGAGGTAAAACGTGTGGATTATTAATTTTAAAGGGGGAAAAACTTTTTTGCTGTTATCCTGTGAAGCTTATTGTTGAATTCTAAAGTTTGAAAGCCTTGTTTCTACTTCAGCTGTACTTTTTTTTTTTTTTTTTTTTGTTATGTCATGAACAGATTTTTTTAGGCCTAGCTTGGTAACCCTCTAGTTTTTTAAAATTAAGCCTATTTTCTTAAGTAAAAAAGCTGAATTCTTAGCCAACAAGTTTTTAAAAGACTATTTTTTTTAGTTTTTAGAACTTGGCCTAGTTTTTTTAAAATATTGATAGAAAAATACATAAAATGTGTTTATAAGTTTGATTTTCAAAAACTAAAAATCCAAAACCAAATAGATTTCAAATGGAGCCTTAGTGTTTGATGAAAGGATGGTTGTGAAGTTTATCGCTGATATAATCCTCGGGAGAGTGTTACTGGGGGACATATTATTTTATCATTGAAGGATAATGATCTCTTTTTCCCTCTTATCAGATTATAAAAATCAAAGATATAATTCCTCCACCAGAGAAGGAGAAATTCAATGATGTGTATATTGTGTATGAATTAATGGACACTGACCTGCACCAGATCATTCGCTCTTCCCAGGCTTTGACAGACGATCACTGTCAGGTAAGGGATACTTATCATGCTATCACTGATGTTTACTTGGAAACTCTTACATTCGAGTGAGATATTAGCGAAGTCTGCTGTAATATTTTATGACAAAGTTTCATTTGGAGTATAAATTTGTTGGAGCAACAATCTGAAACAGTTTGAATGATTTTTGTGTTTGGTTAGAGGACCTGTCTCTCGTTAAGCCTGTTAAAGTGGTATCTGTTTATTGTTTGGACAGTATTTCTTATACCAATTATTGCGAGGTTTGAAGTATTTACACTCTGCAAATGTCTTGCACCGAGATCTAAAACCAAGCAACCTGCTTCTTAATGCAAATTGTGACCTCAAGATTTGTGACTTTGGGTTAGCAAGAACAACATCAGAGACAGATTTCATGACAGAATATGTTGTAACGCGATGGTATCGAGCCCCTGAGCTACTACTCAACACTTCTGAATACACTGCAGCGATAGATATTTGGTCCGTTGGATGTATTCTCATGGAAATTCTTAGGAGGGAGCCCCTGTTCCCTGGTAAAGACTATGTGCAGCAATTGGGGCTTATAATTGAGGTAATTAATCACTGGCTTCCAAGTCTCTTCGTGCTGTTTAAAATTATGCATGTGGCACTGAGGTGATCTCATTGTTTTTTTACAATGATTATTTTCATAAACAACCTGAATTCTTAATTGTGATGTTTTTTAATGGAATTATTTCTTTATCACTACCTGAAAAGCAGTTGCTAGGTTCACCAGATGATTCAGATCTCGGGTTTCTTAGAAGTGATAATGCAAGGAAGTATGTTAAGCAACTTCCCCATTTCCCAAAACAACCGCTGACCGAAAAGTTCCCAGATCTTCCTCCACTGGCAGTTGATCTTGCAGAGAGGATGCTGCTCTTTGATCCAAGCAAGAGAATAACTGGTAATGCACCTACCTTTGCATCTATAACATTACTTAAAGAACCTCTTTTCCCCCTCTCCTACATTTTCATGATCTGAGATGTATTTCTATCGTGTTTCCAAGTTGAGGAGGCAATGAATCACCCATATATAGTAAGTCTTCATGAAATCAATGAAGAACCAACCTGCCCTTCTCCTTTCAACTTCGATTTCGAACAGGCATCCTTGGATGAAGAAGACATAAAAGAACTTATATGGAGGGAGTCTCTTAAGTTCAATCCAAATCACATTTAGTTTCAAACATTTTGTTCCTCCTCAGTTTATTGGGTCTTGATGGAAGAGTTGATCTCGTTACGAAGATTAAGCGAGTATAGTAGTGTGAATTGATGTCGTGTCAGCGGCTGGCAAGTTGGCAACATAACTGGAAACAAATAGTTCTTGTATATAATCATGGAAAAGGTTCATTTATCAGGGGTTGAAAGCGTTCTATATTCACCTTCTTCTGGAATCCTGCATCCAGTGAAGGCCGGTGTGACATAAAAAGGTGCCGCATCTTCCTCGCCGTTTGGATCGAGTTCTTTTGCCTATGTTTTTTTTATTACACTAGGAAGTGAAATTTGTGACTCCTACAGGAAGAGAAAAAAACAGATATACAATGTAATGGTTTGCTGCTCTTCCTGGTATTGTTGCTAGTGTTTAATACCAAAACATACAGTTTTGTTTTTGAGATTGTGAGTTCATGTTGACAATGGAATTACTGTTACCAACATGCAGAGGGTGATTTTGTAATTGTTAGTGTGGACTTCAATAAGAATCGCTTTAAGAAATTTCAATTTCTTGAAAGTGTGTTTATAAGAAACGAAATTTTTATTGTATGTCTTTGTTTTGACTACAAATATAGTAGATTTTTCAGTTGATTTATGCATAGTATCATTCAAATTAGAAAAGTTGAGTGTTCTCATGTAATTCGGTAGG

mRNA sequence

ATTCAAATTTCAAAGTTTCAAAAGATGAAAGACGCGGAAAGTAAAATTCCAGATGAGATGGCGACGCTTTTTCTCCTTCTTCTTCCATCAATTCCAAACCAAACCCTTCTTTTTAACGCTCTCTCTCTTTCTCTCTTTCGCCATTATCACTGAAAACCTTCACTTCTTCCTTCTTCACCCTTTTTCAGACCCAACAACAAGAAAATGGAGAATGATTCTTCTTCTTCCGCTATGGATATCAAAGGCATTCCCACTTACGACTCCAAATACTTACTCTACAATGTTCTCGGCAACTTCTTTGAGATCTCTGCCAAGTACTCCCCTTCAATTCAACCTGTTGGACGCGGTGCTTACGGCATTGTTTGTTGTACGACCAATTCTGAGACGAAAGAAGAGGTAGCGATAAAGAAGATTGGGAATGCTTTTGATAATAGGATTGATGCCAAGAGGACGCTTCGTGAGATTAAACTCCTATGCCATATGGATCATGACAATATTATAAAAATCAAAGATATAATTCCTCCACCAGAGAAGGAGAAATTCAATGATGTGTATATTGTGTATGAATTAATGGACACTGACCTGCACCAGATCATTCGCTCTTCCCAGGCTTTGACAGACGATCACTGTCAGTATTTCTTATACCAATTATTGCGAGGTTTGAAGTATTTACACTCTGCAAATGTCTTGCACCGAGATCTAAAACCAAGCAACCTGCTTCTTAATGCAAATTGTGACCTCAAGATTTGTGACTTTGGGTTAGCAAGAACAACATCAGAGACAGATTTCATGACAGAATATGTTGTAACGCGATGGTATCGAGCCCCTGAGCTACTACTCAACACTTCTGAATACACTGCAGCGATAGATATTTGGTCCGTTGGATGTATTCTCATGGAAATTCTTAGGAGGGAGCCCCTGTTCCCTGGTAAAGACTATGTGCAGCAATTGGGGCTTATAATTGAGTTGCTAGGTTCACCAGATGATTCAGATCTCGGGTTTCTTAGAAGTGATAATGCAAGGAAGTATGTTAAGCAACTTCCCCATTTCCCAAAACAACCGCTGACCGAAAAGTTCCCAGATCTTCCTCCACTGGCAGTTGATCTTGCAGAGAGGATGCTGCTCTTTGATCCAAGCAAGAGAATAACTGTTGAGGAGGCAATGAATCACCCATATATAGTAAGTCTTCATGAAATCAATGAAGAACCAACCTGCCCTTCTCCTTTCAACTTCGATTTCGAACAGGCATCCTTGGATGAAGAAGACATAAAAGAACTTATATGGAGGGAGTCTCTTAAGTTCAATCCAAATCACATTTAGTTTCAAACATTTTGTTCCTCCTCAGTTTATTGGGTCTTGATGGAAGAGTTGATCTCGTTACGAAGATTAAGCGAGTATAGTAGTGTGAATTGATGTCGTGTCAGCGGCTGGCAAGTTGGCAACATAACTGGAAACAAATAGTTCTTGTATATAATCATGGAAAAGGTTCATTTATCAGGGGTTGAAAGCGTTCTATATTCACCTTCTTCTGGAATCCTGCATCCAGTGAAGGCCGGTGTGACATAAAAAGGTGCCGCATCTTCCTCGCCGTTTGGATCGAGTTCTTTTGCCTATGTTTTTTTTATTACACTAGGAAGTGAAATTTGTGACTCCTACAGGAAGAGAAAAAAACAGATATACAATGTAATGGTTTGCTGCTCTTCCTGGTATTGTTGCTAGTGTTTAATACCAAAACATACAGTTTTGTTTTTGAGATTGTGAGTTCATGTTGACAATGGAATTACTGTTACCAACATGCAGAGGGTGATTTTGTAATTGTTAGTGTGGACTTCAATAAGAATCGCTTTAAGAAATTTCAATTTCTTGAAAGTGTGTTTATAAGAAACGAAATTTTTATTGTATGTCTTTGTTTTGACTACAAATATAGTAGATTTTTCAGTTGATTTATGCATAGTATCATTCAAATTAGAAAAGTTGAGTGTTCTCATGTAATTCGGTAGG

Coding sequence (CDS)

ATGGAGAATGATTCTTCTTCTTCCGCTATGGATATCAAAGGCATTCCCACTTACGACTCCAAATACTTACTCTACAATGTTCTCGGCAACTTCTTTGAGATCTCTGCCAAGTACTCCCCTTCAATTCAACCTGTTGGACGCGGTGCTTACGGCATTGTTTGTTGTACGACCAATTCTGAGACGAAAGAAGAGGTAGCGATAAAGAAGATTGGGAATGCTTTTGATAATAGGATTGATGCCAAGAGGACGCTTCGTGAGATTAAACTCCTATGCCATATGGATCATGACAATATTATAAAAATCAAAGATATAATTCCTCCACCAGAGAAGGAGAAATTCAATGATGTGTATATTGTGTATGAATTAATGGACACTGACCTGCACCAGATCATTCGCTCTTCCCAGGCTTTGACAGACGATCACTGTCAGTATTTCTTATACCAATTATTGCGAGGTTTGAAGTATTTACACTCTGCAAATGTCTTGCACCGAGATCTAAAACCAAGCAACCTGCTTCTTAATGCAAATTGTGACCTCAAGATTTGTGACTTTGGGTTAGCAAGAACAACATCAGAGACAGATTTCATGACAGAATATGTTGTAACGCGATGGTATCGAGCCCCTGAGCTACTACTCAACACTTCTGAATACACTGCAGCGATAGATATTTGGTCCGTTGGATGTATTCTCATGGAAATTCTTAGGAGGGAGCCCCTGTTCCCTGGTAAAGACTATGTGCAGCAATTGGGGCTTATAATTGAGTTGCTAGGTTCACCAGATGATTCAGATCTCGGGTTTCTTAGAAGTGATAATGCAAGGAAGTATGTTAAGCAACTTCCCCATTTCCCAAAACAACCGCTGACCGAAAAGTTCCCAGATCTTCCTCCACTGGCAGTTGATCTTGCAGAGAGGATGCTGCTCTTTGATCCAAGCAAGAGAATAACTGTTGAGGAGGCAATGAATCACCCATATATAGTAAGTCTTCATGAAATCAATGAAGAACCAACCTGCCCTTCTCCTTTCAACTTCGATTTCGAACAGGCATCCTTGGATGAAGAAGACATAAAAGAACTTATATGGAGGGAGTCTCTTAAGTTCAATCCAAATCACATTTAG

Protein sequence

MENDSSSSAMDIKGIPTYDSKYLLYNVLGNFFEISAKYSPSIQPVGRGAYGIVCCTTNSETKEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVYELMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYVQQLGLIIELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDLAERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIWRESLKFNPNHI
Homology
BLAST of Bhi08G000301 vs. TAIR 10
Match: AT1G07880.2 (Protein kinase superfamily protein )

HSP 1 Score: 613.6 bits (1581), Expect = 1.0e-175
Identity = 288/353 (81.59%), Postives = 321/353 (90.93%), Query Frame = 0

Query: 14  GIPTYDSKYLLYNVLGNFFEISAKYSPSIQPVGRGAYGIVCCTTNSETKEEVAIKKIGNA 73
           GI TYD +Y++YNVLGN FE+S+KY P I+P+GRGAYGIVCC TNSET EEVAIKKI NA
Sbjct: 8   GILTYDGRYVMYNVLGNIFELSSKYIPPIEPIGRGAYGIVCCATNSETNEEVAIKKIANA 67

Query: 74  FDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVYELMDTDLHQIIRS 133
           FDNR+DAKRTLREIKLL HMDHDN+IKIKDII  PEKE+F DVYIVYELMDTDLHQIIRS
Sbjct: 68  FDNRVDAKRTLREIKLLSHMDHDNVIKIKDIIELPEKERFEDVYIVYELMDTDLHQIIRS 127

Query: 134 SQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGLARTTSET 193
           +Q LTDDHCQYFLYQ+LRGLKY+HSANVLHRDLKPSNL+LN NCDLKICDFGLART++ET
Sbjct: 128 TQTLTDDHCQYFLYQILRGLKYIHSANVLHRDLKPSNLVLNTNCDLKICDFGLARTSNET 187

Query: 194 DFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYVQQLGLII 253
           + MTEYVVTRWYRAPELLLN+SEYT AIDIWSVGCI MEILRRE LFPGKDYVQQL LI 
Sbjct: 188 EIMTEYVVTRWYRAPELLLNSSEYTGAIDIWSVGCIFMEILRRETLFPGKDYVQQLKLIT 247

Query: 254 ELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDLAERMLLFDPSKR 313
           ELLGSPDDSDL FLRSDNARKYVKQLPH  KQ   EKFP++ P+A+DLAE+ML+FDPSKR
Sbjct: 248 ELLGSPDDSDLDFLRSDNARKYVKQLPHVQKQSFREKFPNISPMALDLAEKMLVFDPSKR 307

Query: 314 ITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIWRESLKF 367
           ITV+EA+  PY+ SLHEINEEPTCP+PF+FDFE+ +LDE+DIKEL+WRESL F
Sbjct: 308 ITVDEALKQPYLASLHEINEEPTCPTPFSFDFEETALDEQDIKELVWRESLHF 360

BLAST of Bhi08G000301 vs. TAIR 10
Match: AT4G01370.1 (MAP kinase 4 )

HSP 1 Score: 575.1 bits (1481), Expect = 4.1e-164
Identity = 274/364 (75.27%), Postives = 312/364 (85.71%), Query Frame = 0

Query: 5   SSSSAMDIKGIPTYDSKYLLYNVLGNFFEISAKYSPSIQPVGRGAYGIVCCTTNSETKEE 64
           SS      KG+ T+   Y+ YNV GN FE+S KY P ++P+GRGAYGIVC  TNSET EE
Sbjct: 9   SSGDQSSSKGVATHGGSYVQYNVYGNLFEVSRKYVPPLRPIGRGAYGIVCAATNSETGEE 68

Query: 65  VAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVYELMD 124
           VAIKKIGNAFDN IDAKRTLREIKLL HMDH+N+I +KDII PP++E FNDVYIVYELMD
Sbjct: 69  VAIKKIGNAFDNIIDAKRTLREIKLLKHMDHENVIAVKDIIKPPQRENFNDVYIVYELMD 128

Query: 125 TDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDF 184
           TDLHQIIRS+Q LTDDHC++FLYQLLRGLKY+HSANVLHRDLKPSNLLLNANCDLK+ DF
Sbjct: 129 TDLHQIIRSNQPLTDDHCRFFLYQLLRGLKYVHSANVLHRDLKPSNLLLNANCDLKLGDF 188

Query: 185 GLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKD 244
           GLART SETDFMTEYVVTRWYRAPELLLN SEYTAAIDIWSVGCIL E + REPLFPGKD
Sbjct: 189 GLARTKSETDFMTEYVVTRWYRAPELLLNCSEYTAAIDIWSVGCILGETMTREPLFPGKD 248

Query: 245 YVQQLGLIIELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDLAER 304
           YV QL LI EL+GSPDDS LGFLRSDNAR+YV+QLP +P+Q    +FP++   AVDL E+
Sbjct: 249 YVHQLRLITELIGSPDDSSLGFLRSDNARRYVRQLPQYPRQNFAARFPNMSAGAVDLLEK 308

Query: 305 MLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIWRESL 364
           ML+FDPS+RITV+EA+ HPY+  LH+INEEP C  PFNFDFEQ +L EE+IKELI+RE++
Sbjct: 309 MLVFDPSRRITVDEALCHPYLAPLHDINEEPVCVRPFNFDFEQPTLTEENIKELIYRETV 368

Query: 365 KFNP 369
           KFNP
Sbjct: 369 KFNP 372

BLAST of Bhi08G000301 vs. TAIR 10
Match: AT1G01560.2 (MAP kinase 11 )

HSP 1 Score: 550.1 bits (1416), Expect = 1.4e-156
Identity = 265/356 (74.44%), Postives = 303/356 (85.11%), Query Frame = 0

Query: 13  KGIPTYDSKYLLYNVLGNFFEISAKYSPSIQPVGRGAYGIVCCTTNSETKEEVAIKKIGN 72
           +G+     +Y+ YNV GN FE+S KY P ++P+GRGA GIVC   NSET EEVAIKKIGN
Sbjct: 14  RGVSINGGRYVQYNVYGNLFEVSKKYVPPLRPIGRGASGIVCAAWNSETGEEVAIKKIGN 73

Query: 73  AFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVYELMDTDLHQIIR 132
           AF N IDAKRTLREIKLL HMDHDN+I I DII PP+ + FNDV+IVYELMDTDLH IIR
Sbjct: 74  AFGNIIDAKRTLREIKLLKHMDHDNVIAIIDIIRPPQPDNFNDVHIVYELMDTDLHHIIR 133

Query: 133 SSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGLARTTSE 192
           S+Q LTDDH ++FLYQLLRGLKY+HSANVLHRDLKPSNLLLNANCDLKI DFGLART SE
Sbjct: 134 SNQPLTDDHSRFFLYQLLRGLKYVHSANVLHRDLKPSNLLLNANCDLKIGDFGLARTKSE 193

Query: 193 TDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYVQQLGLI 252
           TDFMTEYVVTRWYRAPELLLN SEYTAAIDIWSVGCIL EI+ REPLFPG+DYVQQL LI
Sbjct: 194 TDFMTEYVVTRWYRAPELLLNCSEYTAAIDIWSVGCILGEIMTREPLFPGRDYVQQLRLI 253

Query: 253 IELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDLAERMLLFDPSK 312
            EL+GSPDDS LGFLRSDNAR+YV+QLP +P+Q    +FP++   AVDL ++ML+FDP++
Sbjct: 254 TELIGSPDDSSLGFLRSDNARRYVRQLPQYPRQNFAARFPNMSVNAVDLLQKMLVFDPNR 313

Query: 313 RITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIWRESLKFNP 369
           RITV+EA+ HPY+  LHE NEEP C  PF+FDFEQ SL EE+IKELI+RES+KFNP
Sbjct: 314 RITVDEALCHPYLAPLHEYNEEPVCVRPFHFDFEQPSLTEENIKELIYRESVKFNP 369

BLAST of Bhi08G000301 vs. TAIR 10
Match: AT4G11330.1 (MAP kinase 5 )

HSP 1 Score: 541.2 bits (1393), Expect = 6.5e-154
Identity = 253/368 (68.75%), Postives = 307/368 (83.42%), Query Frame = 0

Query: 1   MENDSSSSAMDIKGIPTYDSKYLLYNVLGNFFEISAKYSPSIQPVGRGAYGIVCCTTNSE 60
           +E+ +     +IKG+  +  +Y  YNV GN FE+S KY P I+P+GRGAYG VC   +SE
Sbjct: 5   IESATDLGDTNIKGVLVHGGRYFQYNVYGNLFEVSNKYVPPIRPIGRGAYGFVCAAVDSE 64

Query: 61  TKEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVY 120
           T EE+AIKKIG AFDN++DAKRTLREIKLL H++H+N++ IKDII PP+KE F DVYIV+
Sbjct: 65  THEEIAIKKIGKAFDNKVDAKRTLREIKLLRHLEHENVVVIKDIIRPPKKEDFVDVYIVF 124

Query: 121 ELMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLK 180
           ELMDTDLHQIIRS+Q+L DDHCQYFLYQ+LRGLKY+HSANVLHRDLKPSNLLLN+NCDLK
Sbjct: 125 ELMDTDLHQIIRSNQSLNDDHCQYFLYQILRGLKYIHSANVLHRDLKPSNLLLNSNCDLK 184

Query: 181 ICDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLF 240
           I DFGLARTTSET++MTEYVVTRWYRAPELLLN+SEYT+AID+WSVGCI  EI+ REPLF
Sbjct: 185 ITDFGLARTTSETEYMTEYVVTRWYRAPELLLNSSEYTSAIDVWSVGCIFAEIMTREPLF 244

Query: 241 PGKDYVQQLGLIIELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVD 300
           PGKDYV QL LI EL+GSPD + L FLRS NARKYVK+LP FP+Q  + +FP +   A+D
Sbjct: 245 PGKDYVHQLKLITELIGSPDGASLEFLRSANARKYVKELPKFPRQNFSARFPSMNSTAID 304

Query: 301 LAERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIW 360
           L E+ML+FDP KRITVEEA+ +PY+ +LH++N+EP C + F+F FE  S  EE+IKEL+W
Sbjct: 305 LLEKMLVFDPVKRITVEEALCYPYLSALHDLNDEPVCSNHFSFHFEDPSSTEEEIKELVW 364

Query: 361 RESLKFNP 369
            ES+KFNP
Sbjct: 365 LESVKFNP 372

BLAST of Bhi08G000301 vs. TAIR 10
Match: AT2G43790.1 (MAP kinase 6 )

HSP 1 Score: 539.3 bits (1388), Expect = 2.5e-153
Identity = 248/362 (68.51%), Postives = 310/362 (85.64%), Query Frame = 0

Query: 12  IKGIP---TYDSKYLLYNVLGNFFEISAKYSPSIQPVGRGAYGIVCCTTNSETKEEVAIK 71
           I+ IP   ++  +++ YN+ GN FE++AKY P I P+G+GAYGIVC   NSET E VAIK
Sbjct: 33  IENIPATLSHGGRFIQYNIFGNIFEVTAKYKPPIMPIGKGAYGIVCSAMNSETNESVAIK 92

Query: 72  KIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVYELMDTDLH 131
           KI NAFDN+IDAKRTLREIKLL HMDH+NI+ I+DIIPPP +  FNDVYI YELMDTDLH
Sbjct: 93  KIANAFDNKIDAKRTLREIKLLRHMDHENIVAIRDIIPPPLRNAFNDVYIAYELMDTDLH 152

Query: 132 QIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGLAR 191
           QIIRS+QAL+++HCQYFLYQ+LRGLKY+HSANVLHRDLKPSNLLLNANCDLKICDFGLAR
Sbjct: 153 QIIRSNQALSEEHCQYFLYQILRGLKYIHSANVLHRDLKPSNLLLNANCDLKICDFGLAR 212

Query: 192 TTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYVQQ 251
            TSE+DFMTEYVVTRWYRAPELLLN+S+YTAAID+WSVGCI ME++ R+PLFPG+D+V Q
Sbjct: 213 VTSESDFMTEYVVTRWYRAPELLLNSSDYTAAIDVWSVGCIFMELMDRKPLFPGRDHVHQ 272

Query: 252 LGLIIELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDLAERMLLF 311
           L L++EL+G+P + +L FL ++NA++Y++QLP +P+Q +T+KFP + PLA+DL E+ML F
Sbjct: 273 LRLLMELIGTPSEEELEFL-NENAKRYIRQLPPYPRQSITDKFPTVHPLAIDLIEKMLTF 332

Query: 312 DPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIWRESLKFNP 371
           DP +RITV +A+ HPY+ SLH+I++EP C  PFNFDFE  +L EE +KELI+RE+L FNP
Sbjct: 333 DPRRRITVLDALAHPYLNSLHDISDEPECTIPFNFDFENHALSEEQMKELIYREALAFNP 392

BLAST of Bhi08G000301 vs. ExPASy Swiss-Prot
Match: Q40531 (Mitogen-activated protein kinase homolog NTF6 OS=Nicotiana tabacum OX=4097 GN=NTF6 PE=2 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 1.4e-185
Identity = 305/369 (82.66%), Postives = 345/369 (93.50%), Query Frame = 0

Query: 1   MENDSSSSAMDIKGIPTYDSKYLLYNVLGNFFEISAKYSPSIQPVGRGAYGIVCCTTNSE 60
           MEN+++   ++IKGIPT++ KY+ YNVLGNFFE+++KY P IQPVGRGAYG+VCC TNSE
Sbjct: 1   MENETNEK-LEIKGIPTHEGKYVEYNVLGNFFEVTSKYIPPIQPVGRGAYGMVCCATNSE 60

Query: 61  TKEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVY 120
           TKEEVAIKKIGNAF+NRIDAKRTLREIKLL HMDH+NIIKIKDI+ PP++E+FNDVYIVY
Sbjct: 61  TKEEVAIKKIGNAFENRIDAKRTLREIKLLSHMDHENIIKIKDIVRPPDREEFNDVYIVY 120

Query: 121 ELMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLK 180
           ELMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKY+HSANVLHRDLKPSNLLLNANCDLK
Sbjct: 121 ELMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYVHSANVLHRDLKPSNLLLNANCDLK 180

Query: 181 ICDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLF 240
           ICDFGLARTTSE DFMTEYVVTRWYRAPELLLN +EYTAAIDIWSVGCILME+++REPLF
Sbjct: 181 ICDFGLARTTSEADFMTEYVVTRWYRAPELLLNCTEYTAAIDIWSVGCILMELIKREPLF 240

Query: 241 PGKDYVQQLGLIIELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVD 300
           PG+DY QQLGLII LLGSP+DSDLGFLRSDNARKYVK LP  P+ P ++KFPD+ PLA+D
Sbjct: 241 PGRDYAQQLGLIIALLGSPEDSDLGFLRSDNARKYVKHLPRVPRHPFSQKFPDVSPLALD 300

Query: 301 LAERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIW 360
           LAERML+FDP+KRITVE+A+NHP+++SLHEINEEP C SPFNFDFEQASL E+DIKELIW
Sbjct: 301 LAERMLVFDPAKRITVEDALNHPFLISLHEINEEPVCDSPFNFDFEQASLSEDDIKELIW 360

Query: 361 RESLKFNPN 370
            E+LKF+PN
Sbjct: 361 NEALKFDPN 368

BLAST of Bhi08G000301 vs. ExPASy Swiss-Prot
Match: Q9LQQ9 (Mitogen-activated protein kinase 13 OS=Arabidopsis thaliana OX=3702 GN=MPK13 PE=1 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 1.5e-174
Identity = 288/353 (81.59%), Postives = 321/353 (90.93%), Query Frame = 0

Query: 14  GIPTYDSKYLLYNVLGNFFEISAKYSPSIQPVGRGAYGIVCCTTNSETKEEVAIKKIGNA 73
           GI TYD +Y++YNVLGN FE+S+KY P I+P+GRGAYGIVCC TNSET EEVAIKKI NA
Sbjct: 8   GILTYDGRYVMYNVLGNIFELSSKYIPPIEPIGRGAYGIVCCATNSETNEEVAIKKIANA 67

Query: 74  FDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVYELMDTDLHQIIRS 133
           FDNR+DAKRTLREIKLL HMDHDN+IKIKDII  PEKE+F DVYIVYELMDTDLHQIIRS
Sbjct: 68  FDNRVDAKRTLREIKLLSHMDHDNVIKIKDIIELPEKERFEDVYIVYELMDTDLHQIIRS 127

Query: 134 SQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGLARTTSET 193
           +Q LTDDHCQYFLYQ+LRGLKY+HSANVLHRDLKPSNL+LN NCDLKICDFGLART++ET
Sbjct: 128 TQTLTDDHCQYFLYQILRGLKYIHSANVLHRDLKPSNLVLNTNCDLKICDFGLARTSNET 187

Query: 194 DFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYVQQLGLII 253
           + MTEYVVTRWYRAPELLLN+SEYT AIDIWSVGCI MEILRRE LFPGKDYVQQL LI 
Sbjct: 188 EIMTEYVVTRWYRAPELLLNSSEYTGAIDIWSVGCIFMEILRRETLFPGKDYVQQLKLIT 247

Query: 254 ELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDLAERMLLFDPSKR 313
           ELLGSPDDSDL FLRSDNARKYVKQLPH  KQ   EKFP++ P+A+DLAE+ML+FDPSKR
Sbjct: 248 ELLGSPDDSDLDFLRSDNARKYVKQLPHVQKQSFREKFPNISPMALDLAEKMLVFDPSKR 307

Query: 314 ITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIWRESLKF 367
           ITV+EA+  PY+ SLHEINEEPTCP+PF+FDFE+ +LDE+DIKEL+WRESL F
Sbjct: 308 ITVDEALKQPYLASLHEINEEPTCPTPFSFDFEETALDEQDIKELVWRESLHF 360

BLAST of Bhi08G000301 vs. ExPASy Swiss-Prot
Match: A9S9Q8 (Mitogen-activated protein kinase 4b OS=Physcomitrium patens OX=3218 GN=MPK4b PE=1 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 1.1e-166
Identity = 274/361 (75.90%), Postives = 314/361 (86.98%), Query Frame = 0

Query: 11  DIKGIPTYDSKYLLYNVLGNFFEISAKYSPSIQPVGRGAYGIVCCTTNSETKEEVAIKKI 70
           +I+G+PT++ +Y  YN+ GN FE+S KY P I+P+GRGAYGIVC   NSET EEVAIKKI
Sbjct: 15  NIQGVPTHNGEYTQYNIFGNLFEVSRKYVPPIRPIGRGAYGIVCSAVNSETGEEVAIKKI 74

Query: 71  GNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVYELMDTDLHQI 130
           GNAFDNRIDAKRTLREIKLL HMDH+NI+ I+DII PP +E FNDVYIVYELMDTDLHQI
Sbjct: 75  GNAFDNRIDAKRTLREIKLLRHMDHENIVAIRDIIRPPTRENFNDVYIVYELMDTDLHQI 134

Query: 131 IRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGLARTT 190
           IRS+Q LT+DHCQYFLYQLLRGLKY+HSA VLHRDLKPSNLLLNANCDLKICDFGLARTT
Sbjct: 135 IRSNQPLTEDHCQYFLYQLLRGLKYIHSAKVLHRDLKPSNLLLNANCDLKICDFGLARTT 194

Query: 191 SETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYVQQLG 250
           SETDFMTEYVVTRWYRAPELLLN SEYTAAID+WSVGCI ME+L REPLFPG+DYVQQL 
Sbjct: 195 SETDFMTEYVVTRWYRAPELLLNCSEYTAAIDVWSVGCIFMELLNREPLFPGRDYVQQLR 254

Query: 251 LIIELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDLAERMLLFDP 310
           LI EL+GSP+D DLGFLRSDNAR+Y++QLP F +QPL  KFP++ P A+DL E ML FDP
Sbjct: 255 LITELIGSPEDHDLGFLRSDNARRYIRQLPRFARQPLDRKFPNMGPAAIDLVEHMLRFDP 314

Query: 311 SKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIWRESLKFNPNH 370
           ++RITVEEA+ HPY+ +LH+IN+EP C SPF FDFEQ S  EE IKELI  E++ FNP +
Sbjct: 315 ARRITVEEALAHPYLATLHDINDEPICHSPFEFDFEQPSFTEEHIKELIMMEAIAFNPGN 374

Query: 371 I 372
           +
Sbjct: 375 V 375

BLAST of Bhi08G000301 vs. ExPASy Swiss-Prot
Match: Q336X9 (Mitogen-activated protein kinase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=MPK6 PE=2 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 3.6e-165
Identity = 275/358 (76.82%), Postives = 315/358 (87.99%), Query Frame = 0

Query: 12  IKGIPTYDSKYLLYNVLGNFFEISAKYSPSIQPVGRGAYGIVCCTTNSETKEEVAIKKIG 71
           IKG+ T+  +Y+LYNV GNFFE+S+KY+P I+P+GRGAYGIVC   NSE  EEVAIKKIG
Sbjct: 16  IKGMGTHGGRYVLYNVYGNFFEVSSKYAPPIRPIGRGAYGIVCAAVNSENGEEVAIKKIG 75

Query: 72  NAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVYELMDTDLHQII 131
           NAFDN IDAKRTLREIKLL HMDH+NII IKDII PP ++ FNDVYIV ELMDTDLHQII
Sbjct: 76  NAFDNHIDAKRTLREIKLLRHMDHENIIAIKDIIRPPRRDNFNDVYIVSELMDTDLHQII 135

Query: 132 RSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGLARTTS 191
           RS+Q LTDDHCQYFLYQLLRGLKY+HSANVLHRDLKPSNL LNANCDLKI DFGLARTT+
Sbjct: 136 RSNQPLTDDHCQYFLYQLLRGLKYVHSANVLHRDLKPSNLFLNANCDLKIADFGLARTTT 195

Query: 192 ETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYVQQLGL 251
           ETD MTEYVVTRWYRAPELLLN S+YTAAID+WSVGCIL EI+ R+PLFPG+DY+QQL L
Sbjct: 196 ETDLMTEYVVTRWYRAPELLLNCSQYTAAIDVWSVGCILGEIVTRQPLFPGRDYIQQLKL 255

Query: 252 IIELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDLAERMLLFDPS 311
           I EL+GSPDDS LGFLRSDNAR+Y+KQLP +P+Q    +F ++   AVDL E+ML+FDPS
Sbjct: 256 ITELIGSPDDSSLGFLRSDNARRYMKQLPQYPRQDFRLRFRNMSAGAVDLLEKMLVFDPS 315

Query: 312 KRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIWRESLKFNPN 370
           +RITV+EA++HPY+ SLH+INEEPTCP+PF+FDFEQ S  EE IKELIWRESL FNP+
Sbjct: 316 RRITVDEALHHPYLASLHDINEEPTCPAPFSFDFEQPSFTEEHIKELIWRESLAFNPD 373

BLAST of Bhi08G000301 vs. ExPASy Swiss-Prot
Match: Q5J4W4 (Mitogen-activated protein kinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=MPK2 PE=2 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 4.4e-163
Identity = 273/360 (75.83%), Postives = 311/360 (86.39%), Query Frame = 0

Query: 12  IKGIPTYDSKYLLYNVLGNFFEISAKYSPSIQPVGRGAYGIVCCTTNSETKEEVAIKKIG 71
           IKG  T+  +Y+ YNV GN FE+S+KY P I+PVGRGA GI+C   N++T++EVAIKKIG
Sbjct: 34  IKGTLTHGGRYVQYNVYGNLFEVSSKYVPPIRPVGRGACGIICAVVNAQTRQEVAIKKIG 93

Query: 72  NAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVYELMDTDLHQII 131
           NAFDN+IDAKRTLREIKLL HMDHDN+I IKDII PP +E FNDVYIVYELMDTDLH ++
Sbjct: 94  NAFDNQIDAKRTLREIKLLRHMDHDNVISIKDIIRPPRRENFNDVYIVYELMDTDLHHLL 153

Query: 132 RSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGLARTTS 191
           RS+Q LTDDHCQYFLYQ+LRGLKY+HSANVLHRDL+PSNLLLNA CDLKI DFGLARTT+
Sbjct: 154 RSNQPLTDDHCQYFLYQVLRGLKYVHSANVLHRDLRPSNLLLNAKCDLKIGDFGLARTTN 213

Query: 192 ETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYVQQLGL 251
           ETDFM EYVVTRWYRAPELLLN SEYTAAIDIWSVGCIL EI+ REPLFPGKDYV QL L
Sbjct: 214 ETDFMMEYVVTRWYRAPELLLNCSEYTAAIDIWSVGCILGEIVTREPLFPGKDYVHQLRL 273

Query: 252 IIELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDLAERMLLFDPS 311
           I EL+GSPDDS LGFLRSDNAR+YV+ LP +PKQ    +FP +   A+DL ERML+FDPS
Sbjct: 274 ITELIGSPDDSSLGFLRSDNARRYVRSLPQYPKQQFRARFPTMSSGAMDLLERMLVFDPS 333

Query: 312 KRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIWRESLKFNPNHI 371
           KRITV+EA+ HPY+ SLHEI +EP CP+PF+FDFEQ SL EEDIKE+IWRE+LKFNP  I
Sbjct: 334 KRITVDEALCHPYLASLHEIYDEPVCPAPFSFDFEQPSLTEEDIKEIIWREALKFNPEPI 393

BLAST of Bhi08G000301 vs. ExPASy TrEMBL
Match: A0A0A0LS13 (Mitogen-activated protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1G077220 PE=3 SV=1)

HSP 1 Score: 745.0 bits (1922), Expect = 1.5e-211
Identity = 363/371 (97.84%), Postives = 367/371 (98.92%), Query Frame = 0

Query: 1   MENDSSSSAMDIKGIPTYDSKYLLYNVLGNFFEISAKYSPSIQPVGRGAYGIVCCTTNSE 60
           MEND SSSAMDIKG PTYDSKYLLYNVLG+FFE+SAKYSPSIQPVGRGAYGIVCCTTNSE
Sbjct: 1   MEND-SSSAMDIKGTPTYDSKYLLYNVLGSFFEVSAKYSPSIQPVGRGAYGIVCCTTNSE 60

Query: 61  TKEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVY 120
           TKEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPP+KEKFNDVYIVY
Sbjct: 61  TKEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPDKEKFNDVYIVY 120

Query: 121 ELMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLK 180
           ELMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLK
Sbjct: 121 ELMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLK 180

Query: 181 ICDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLF 240
           ICDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLF
Sbjct: 181 ICDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLF 240

Query: 241 PGKDYVQQLGLIIELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVD 300
           PGKDYVQQLGLI ELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPL EKFPDLPPLAVD
Sbjct: 241 PGKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLIEKFPDLPPLAVD 300

Query: 301 LAERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIW 360
           LAERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIW
Sbjct: 301 LAERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIW 360

Query: 361 RESLKFNPNHI 372
           RES+KFNPNHI
Sbjct: 361 RESIKFNPNHI 370

BLAST of Bhi08G000301 vs. ExPASy TrEMBL
Match: A0A1S3CNX4 (Mitogen-activated protein kinase OS=Cucumis melo OX=3656 GN=LOC103502937 PE=3 SV=1)

HSP 1 Score: 742.7 bits (1916), Expect = 7.7e-211
Identity = 362/371 (97.57%), Postives = 366/371 (98.65%), Query Frame = 0

Query: 1   MENDSSSSAMDIKGIPTYDSKYLLYNVLGNFFEISAKYSPSIQPVGRGAYGIVCCTTNSE 60
           MEND SSSAMDIKG PTYDSKYLLYNVLG+FFE+SAKYSPSIQPVGRGAYGIVCCTTNSE
Sbjct: 1   MEND-SSSAMDIKGTPTYDSKYLLYNVLGSFFEVSAKYSPSIQPVGRGAYGIVCCTTNSE 60

Query: 61  TKEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVY 120
           TKEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPP+KEKFNDVYIVY
Sbjct: 61  TKEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPDKEKFNDVYIVY 120

Query: 121 ELMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLK 180
           ELMDTDLHQIIRSSQ LTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLK
Sbjct: 121 ELMDTDLHQIIRSSQDLTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLK 180

Query: 181 ICDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLF 240
           ICDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLF
Sbjct: 181 ICDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLF 240

Query: 241 PGKDYVQQLGLIIELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVD 300
           PGKDYVQQLGLI ELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPL EKFPDLPPLAVD
Sbjct: 241 PGKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLIEKFPDLPPLAVD 300

Query: 301 LAERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIW 360
           LAERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIW
Sbjct: 301 LAERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIW 360

Query: 361 RESLKFNPNHI 372
           RES+KFNPNHI
Sbjct: 361 RESIKFNPNHI 370

BLAST of Bhi08G000301 vs. ExPASy TrEMBL
Match: A0A2D0ULS2 (Mitogen-activated protein kinase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 742.3 bits (1915), Expect = 1.0e-210
Identity = 362/371 (97.57%), Postives = 366/371 (98.65%), Query Frame = 0

Query: 1   MENDSSSSAMDIKGIPTYDSKYLLYNVLGNFFEISAKYSPSIQPVGRGAYGIVCCTTNSE 60
           MEND SSSAMDIKG PTYDSKYLLYNVLG+FFE+SAKYSPSIQPVGRGAYGIVCCTTNSE
Sbjct: 1   MEND-SSSAMDIKGTPTYDSKYLLYNVLGSFFEVSAKYSPSIQPVGRGAYGIVCCTTNSE 60

Query: 61  TKEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVY 120
           TKEEVAIKKIGNAFDNRIDAKRTLREIK LCHMDHDNIIKIKDIIPPP+KEKFNDVYIVY
Sbjct: 61  TKEEVAIKKIGNAFDNRIDAKRTLREIKPLCHMDHDNIIKIKDIIPPPDKEKFNDVYIVY 120

Query: 121 ELMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLK 180
           ELMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLK
Sbjct: 121 ELMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLK 180

Query: 181 ICDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLF 240
           ICDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLF
Sbjct: 181 ICDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLF 240

Query: 241 PGKDYVQQLGLIIELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVD 300
           PGKDYVQQLGLI ELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPL EKFPDLPPLAVD
Sbjct: 241 PGKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLIEKFPDLPPLAVD 300

Query: 301 LAERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIW 360
           LAERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIW
Sbjct: 301 LAERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIW 360

Query: 361 RESLKFNPNHI 372
           RES+KFNPNHI
Sbjct: 361 RESIKFNPNHI 370

BLAST of Bhi08G000301 vs. ExPASy TrEMBL
Match: A0A6J1I0F3 (Mitogen-activated protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111469378 PE=3 SV=1)

HSP 1 Score: 733.4 bits (1892), Expect = 4.7e-208
Identity = 356/371 (95.96%), Postives = 364/371 (98.11%), Query Frame = 0

Query: 1   MENDSSSSAMDIKGIPTYDSKYLLYNVLGNFFEISAKYSPSIQPVGRGAYGIVCCTTNSE 60
           MEND  SSAM+IKG PTYD KYLLYNVLGNFFE+SAKY+PSIQPVGRGAYGIVCCTTNSE
Sbjct: 1   MEND--SSAMEIKGFPTYDGKYLLYNVLGNFFEVSAKYAPSIQPVGRGAYGIVCCTTNSE 60

Query: 61  TKEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVY 120
           TKEEVAIKKIGNAFDNRIDAKRTLREI+LLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVY
Sbjct: 61  TKEEVAIKKIGNAFDNRIDAKRTLREIELLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVY 120

Query: 121 ELMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLK 180
           ELMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLK
Sbjct: 121 ELMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLK 180

Query: 181 ICDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLF 240
           ICDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIW+VGCILMEILRREPLF
Sbjct: 181 ICDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWAVGCILMEILRREPLF 240

Query: 241 PGKDYVQQLGLIIELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVD 300
           PGKDYVQQLGLI ELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPL EKFPDLPPLAVD
Sbjct: 241 PGKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLIEKFPDLPPLAVD 300

Query: 301 LAERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIW 360
           LAERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEE+IKELIW
Sbjct: 301 LAERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEEIKELIW 360

Query: 361 RESLKFNPNHI 372
           R SLKFNPN++
Sbjct: 361 RASLKFNPNYM 369

BLAST of Bhi08G000301 vs. ExPASy TrEMBL
Match: A0A5D3DCH5 (Mitogen-activated protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold359G00340 PE=3 SV=1)

HSP 1 Score: 733.0 bits (1891), Expect = 6.1e-208
Identity = 354/362 (97.79%), Postives = 358/362 (98.90%), Query Frame = 0

Query: 10  MDIKGIPTYDSKYLLYNVLGNFFEISAKYSPSIQPVGRGAYGIVCCTTNSETKEEVAIKK 69
           MDIKG PTYDSKYLLYNVLG+FFE+SAKYSPSIQPVGRGAYGIVCCTTNSETKEEVAIKK
Sbjct: 1   MDIKGTPTYDSKYLLYNVLGSFFEVSAKYSPSIQPVGRGAYGIVCCTTNSETKEEVAIKK 60

Query: 70  IGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVYELMDTDLHQ 129
           IGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPP+KEKFNDVYIVYELMDTDLHQ
Sbjct: 61  IGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPDKEKFNDVYIVYELMDTDLHQ 120

Query: 130 IIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGLART 189
           IIRSSQ LTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGLART
Sbjct: 121 IIRSSQDLTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGLART 180

Query: 190 TSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYVQQL 249
           TSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYVQQL
Sbjct: 181 TSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYVQQL 240

Query: 250 GLIIELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDLAERMLLFD 309
           GLI ELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPL EKFPDLPPLAVDLAERMLLFD
Sbjct: 241 GLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLIEKFPDLPPLAVDLAERMLLFD 300

Query: 310 PSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIWRESLKFNPN 369
           PSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIWRES+KFNPN
Sbjct: 301 PSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIWRESIKFNPN 360

Query: 370 HI 372
           HI
Sbjct: 361 HI 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G07880.21.0e-17581.59Protein kinase superfamily protein [more]
AT4G01370.14.1e-16475.27MAP kinase 4 [more]
AT1G01560.21.4e-15674.44MAP kinase 11 [more]
AT4G11330.16.5e-15468.75MAP kinase 5 [more]
AT2G43790.12.5e-15368.51MAP kinase 6 [more]
Match NameE-valueIdentityDescription
Q405311.4e-18582.66Mitogen-activated protein kinase homolog NTF6 OS=Nicotiana tabacum OX=4097 GN=NT... [more]
Q9LQQ91.5e-17481.59Mitogen-activated protein kinase 13 OS=Arabidopsis thaliana OX=3702 GN=MPK13 PE=... [more]
A9S9Q81.1e-16675.90Mitogen-activated protein kinase 4b OS=Physcomitrium patens OX=3218 GN=MPK4b PE=... [more]
Q336X93.6e-16576.82Mitogen-activated protein kinase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
Q5J4W44.4e-16375.83Mitogen-activated protein kinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
Match NameE-valueIdentityDescription
A0A0A0LS131.5e-21197.84Mitogen-activated protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1G077220 PE=3... [more]
A0A1S3CNX47.7e-21197.57Mitogen-activated protein kinase OS=Cucumis melo OX=3656 GN=LOC103502937 PE=3 SV... [more]
A0A2D0ULS21.0e-21097.57Mitogen-activated protein kinase OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
A0A6J1I0F34.7e-20895.96Mitogen-activated protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111469378 PE=... [more]
A0A5D3DCH56.1e-20897.79Mitogen-activated protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 39..325
e-value: 7.2E-94
score: 327.8
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 42..325
e-value: 8.2E-70
score: 235.2
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 39..325
score: 44.795605
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 22..362
e-value: 6.4E-159
score: 530.1
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 123..352
e-value: 6.4E-159
score: 530.1
NoneNo IPR availablePIRSRPIRSR037921-1PIRSR037921-1coord: 40..237
e-value: 1.1E-12
score: 45.8
NoneNo IPR availablePIRSRPIRSR630616-1PIRSR630616-1coord: 43..244
e-value: 5.2E-28
score: 95.7
NoneNo IPR availablePIRSRPIRSR037921-2PIRSR037921-2coord: 40..237
e-value: 1.1E-12
score: 45.8
NoneNo IPR availablePIRSRPIRSR037568-1PIRSR037568-1coord: 41..243
e-value: 9.4E-14
score: 47.6
NoneNo IPR availablePANTHERPTHR24055:SF438MITOGEN-ACTIVATED PROTEIN KINASE 13coord: 18..370
NoneNo IPR availablePANTHERPTHR24055MITOGEN-ACTIVATED PROTEIN KINASEcoord: 18..370
NoneNo IPR availableCDDcd07858STKc_TEY_MAPKcoord: 32..368
e-value: 0.0
score: 660.218
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 45..69
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 161..173
IPR003527Mitogen-activated protein (MAP) kinase, conserved sitePROSITEPS01351MAPKcoord: 74..177
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 42..331

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi08M000301Bhi08M000301mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010311 lateral root formation
biological_process GO:0000165 MAPK cascade
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0004707 MAP kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity