Bhi08G000287 (gene) Wax gourd (B227) v1

Overview
NameBhi08G000287
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionDEAD-box ATP-dependent RNA helicase 24
Locationchr8: 11951251 .. 11952505 (-)
RNA-Seq ExpressionBhi08G000287
SyntenyBhi08G000287
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTGGGCAATATATCACCTCCAGCGGTGGTGTCATAACTGCTGAAGAACTTGCACCATATCTAAATTTGTCAGAGGGGAACATGGTAAGTTTCAAACTGAAACTGATAGGGGTTGGGGTACCAATGGAAAATATTGATGTCTTTTCAAAGATGTAAACAATTTGGTTAAGGTTTCCAATCATTATTGCTCGACAACTATTTGACAATAGGTGGAATGGAATCAACTCATATTTAAGTGTAATTCAAATCTTAGTTTTCTGTATGAATTTGTTTCATCTTCTTGATGATTGCAGGATGGTGAGTCAGACATTTTACCAGTTCTTTTACGTTTTGATGGCCAACCTGAAATTGATGAAGAGGTATGTTTAACGAGGTATGAAATGCTTGAATGGATACAATTAGCTCAATCTACCCATATTCGATGCCAATAATATGTGAACACAAGTCTGTTGCTAGGTTGAGGCTTGAAGTGTTATGCTAGTTATTTCATCTATTGAGGCTTGAACACAAATCTGAAATTGATGTTAGTTATTTCGTCTATCCCAGAAAGCACAATCTACCCATAGTTGAGGCTTGAACAATGGTTTGAAATTAATGAAGTGTTATTTCGTCTTTAGGGATTAGTGAAGAGGAAGTTTCTTATTACCGGAAGAGTTTGGCTATTCATGTGTCGGGTTTTGACGTTCCCAGGCCAATTAAAACCTTTGAAGGCTTTAGATTTTCCGCTCAACTTATGAATGCTATAAAAAAGCAAGGGTATGAGAAGCCTACTTCTATACAGTGCCAAGCTATGCCAATTGTGCTTTCTGGGAGAGATATCATTAGAATAGCAAAAAATGGTTCGAGTAAGACTGCTGCTTTTGTTTTTCCAATGATTGTTCATATTATGGATCAACTTAAATTTGATCGGCTTAAAGAATTGAAAGCTAGGTGTGAGATAGTTGTTGCCTTTCTTTTTAGTTCTCTTCTTGGGAGAAGTTTTCTAACATTCAGTTTAAATACAAGCTGAACAAAATATAAGCAATAATAATGGTTATATAAGCAGCAAAGATTAACACATATAAAAAGATTAAGGCAATAATGAAGAACAAGTAAAGAGGAGCATAAACTTAAGAATTCTGACTATTAGAATTATGTTCCTTATATTATTTTGGAAATATCCATATGGGTGTGTCAATATTATTTCATTGATGTAGGTGCTCAAAGAGACGAGTTCAAGGTGGGCTACGTTATTCTTGTGGAGGCAGGAGCATAG

mRNA sequence

ATTGGGCAATATATCACCTCCAGCGGTGGTGTCATAACTGCTGAAGAACTTGCACCATATCTAAATTTGTCAGAGGGGAACATGGATGGTGAGTCAGACATTTTACCAGTTCTTTTACGTTTTGATGGCCAACCTGAAATTGATGAAGAGGTATGTTTAACGAGTGTTATTTCGTCTTTAGGGATTAGTGAAGAGGAAGTTTCTTATTACCGGAAGAGTTTGGCTATTCATGTGTCGGGTTTTGACGTTCCCAGGCCAATTAAAACCTTTGAAGGCTTTAGATTTTCCGCTCAACTTATGAATGCTATAAAAAAGCAAGGGTATGAGAAGCCTACTTCTATACAGTGCCAAGCTATGCCAATTGTGCTTTCTGGGAGAGATATCATTAGAATAGCAAAAAATGGTTCGAGTGCTCAAAGAGACGAGTTCAAGGTGGGCTACGTTATTCTTGTGGAGGCAGGAGCATAG

Coding sequence (CDS)

ATTGGGCAATATATCACCTCCAGCGGTGGTGTCATAACTGCTGAAGAACTTGCACCATATCTAAATTTGTCAGAGGGGAACATGGATGGTGAGTCAGACATTTTACCAGTTCTTTTACGTTTTGATGGCCAACCTGAAATTGATGAAGAGGTATGTTTAACGAGTGTTATTTCGTCTTTAGGGATTAGTGAAGAGGAAGTTTCTTATTACCGGAAGAGTTTGGCTATTCATGTGTCGGGTTTTGACGTTCCCAGGCCAATTAAAACCTTTGAAGGCTTTAGATTTTCCGCTCAACTTATGAATGCTATAAAAAAGCAAGGGTATGAGAAGCCTACTTCTATACAGTGCCAAGCTATGCCAATTGTGCTTTCTGGGAGAGATATCATTAGAATAGCAAAAAATGGTTCGAGTGCTCAAAGAGACGAGTTCAAGGTGGGCTACGTTATTCTTGTGGAGGCAGGAGCATAG

Protein sequence

IGQYITSSGGVITAEELAPYLNLSEGNMDGESDILPVLLRFDGQPEIDEEVCLTSVISSLGISEEEVSYYRKSLAIHVSGFDVPRPIKTFEGFRFSAQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIRIAKNGSSAQRDEFKVGYVILVEAGA
Homology
BLAST of Bhi08G000287 vs. TAIR 10
Match: AT2G47330.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 110.9 bits (276), Expect = 9.1e-25
Identity = 52/76 (68.42%), Postives = 64/76 (84.21%), Query Frame = 0

Query: 61  GISEEEVSYYRKSLAIHVSGFDVPRPIKTFEGFRFSAQLMNAIKKQGYEKPTSIQCQAMP 120
           G++E+E + YR+ L I VSGFDV RP+KTFE   FS+Q+M+AIKKQ YEKPT+IQCQA+P
Sbjct: 201 GMTEQETTDYRQRLGIRVSGFDVHRPVKTFEDCGFSSQIMSAIKKQAYEKPTAIQCQALP 260

Query: 121 IVLSGRDIIRIAKNGS 137
           IVLSGRD+I IAK GS
Sbjct: 261 IVLSGRDVIGIAKTGS 276

BLAST of Bhi08G000287 vs. TAIR 10
Match: AT1G20920.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 80.9 bits (198), Expect = 1.0e-15
Identity = 37/75 (49.33%), Postives = 54/75 (72.00%), Query Frame = 0

Query: 62  ISEEEVSYYRKSLAIHVSGFDVPRPIKTFEGFRFSAQLMNAIKKQGYEKPTSIQCQAMPI 121
           +++EEV+ YRK L + V G DVPRPIK +     ++++++ +KK  YEKP  IQ QA+PI
Sbjct: 503 MTQEEVNTYRKELELKVHGKDVPRPIKFWHQTGLTSKILDTMKKLNYEKPMPIQTQALPI 562

Query: 122 VLSGRDIIRIAKNGS 137
           ++SGRD I +AK GS
Sbjct: 563 IMSGRDCIGVAKTGS 577

BLAST of Bhi08G000287 vs. TAIR 10
Match: AT1G20920.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 80.9 bits (198), Expect = 1.0e-15
Identity = 37/75 (49.33%), Postives = 54/75 (72.00%), Query Frame = 0

Query: 62  ISEEEVSYYRKSLAIHVSGFDVPRPIKTFEGFRFSAQLMNAIKKQGYEKPTSIQCQAMPI 121
           +++EEV+ YRK L + V G DVPRPIK +     ++++++ +KK  YEKP  IQ QA+PI
Sbjct: 165 MTQEEVNTYRKELELKVHGKDVPRPIKFWHQTGLTSKILDTMKKLNYEKPMPIQTQALPI 224

Query: 122 VLSGRDIIRIAKNGS 137
           ++SGRD I +AK GS
Sbjct: 225 IMSGRDCIGVAKTGS 239

BLAST of Bhi08G000287 vs. TAIR 10
Match: AT3G09620.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 76.6 bits (187), Expect = 1.9e-14
Identity = 34/75 (45.33%), Postives = 53/75 (70.67%), Query Frame = 0

Query: 62  ISEEEVSYYRKSLAIHVSGFDVPRPIKTFEGFRFSAQLMNAIKKQGYEKPTSIQCQAMPI 121
           ++++ V+ YRK L + V G DVPRPI+ +     ++++++ +KK  YEKP  IQ QA+PI
Sbjct: 370 MTQDAVNAYRKELELKVHGKDVPRPIQFWHQTGLTSKILDTLKKLNYEKPMPIQAQALPI 429

Query: 122 VLSGRDIIRIAKNGS 137
           ++SGRD I +AK GS
Sbjct: 430 IMSGRDCIGVAKTGS 444

BLAST of Bhi08G000287 vs. TAIR 10
Match: AT5G03900.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 73.2 bits (178), Expect = 2.1e-13
Identity = 37/52 (71.15%), Postives = 44/52 (84.62%), Query Frame = 0

Query: 1   IGQYITSSGGVITAEELAPYLNL--SEGNMDGESDILPVLLRFDGQPEIDEE 51
           IGQYITS GGV+ A+ELAPYL++  S+  M+ ES ILPVLLRFDGQPE+DEE
Sbjct: 281 IGQYITSRGGVVAADELAPYLDVPSSKSAMNDESYILPVLLRFDGQPELDEE 332

BLAST of Bhi08G000287 vs. ExPASy Swiss-Prot
Match: Q10MH8 (DEAD-box ATP-dependent RNA helicase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0308500 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 1.8e-25
Identity = 58/76 (76.32%), Postives = 64/76 (84.21%), Query Frame = 0

Query: 61  GISEEEVSYYRKSLAIHVSGFDVPRPIKTFEGFRFSAQLMNAIKKQGYEKPTSIQCQAMP 120
           G+SE+EV+ Y KSLAI VSGFDVPRPIK+F    F  QLMNAI KQGYEKPT+IQCQA+P
Sbjct: 192 GMSEQEVADYMKSLAIRVSGFDVPRPIKSFADCGFPVQLMNAIAKQGYEKPTTIQCQALP 251

Query: 121 IVLSGRDIIRIAKNGS 137
           IVLSGRDII IAK GS
Sbjct: 252 IVLSGRDIIGIAKTGS 267

BLAST of Bhi08G000287 vs. ExPASy Swiss-Prot
Match: O22907 (DEAD-box ATP-dependent RNA helicase 24 OS=Arabidopsis thaliana OX=3702 GN=RH24 PE=1 SV=2)

HSP 1 Score: 110.9 bits (276), Expect = 1.3e-23
Identity = 52/76 (68.42%), Postives = 64/76 (84.21%), Query Frame = 0

Query: 61  GISEEEVSYYRKSLAIHVSGFDVPRPIKTFEGFRFSAQLMNAIKKQGYEKPTSIQCQAMP 120
           G++E+E + YR+ L I VSGFDV RP+KTFE   FS+Q+M+AIKKQ YEKPT+IQCQA+P
Sbjct: 201 GMTEQETTDYRQRLGIRVSGFDVHRPVKTFEDCGFSSQIMSAIKKQAYEKPTAIQCQALP 260

Query: 121 IVLSGRDIIRIAKNGS 137
           IVLSGRD+I IAK GS
Sbjct: 261 IVLSGRDVIGIAKTGS 276

BLAST of Bhi08G000287 vs. ExPASy Swiss-Prot
Match: Q84UQ1 (DEAD-box ATP-dependent RNA helicase 42 OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0159900 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 4.4e-16
Identity = 41/75 (54.67%), Postives = 56/75 (74.67%), Query Frame = 0

Query: 62  ISEEEVSYYRKSLAIHVSGFDVPRPIKTFEGFRFSAQLMNAIKKQGYEKPTSIQCQAMPI 121
           ++ EEV+ YRK L + V G DVP+PIKT+     +++L++ IKK G+EKP SIQ QA+PI
Sbjct: 398 MAAEEVAAYRKQLELKVHGKDVPKPIKTWVQSGLTSKLLDTIKKLGFEKPMSIQAQALPI 457

Query: 122 VLSGRDIIRIAKNGS 137
           ++SGRD I IAK GS
Sbjct: 458 IMSGRDCIGIAKTGS 472

BLAST of Bhi08G000287 vs. ExPASy Swiss-Prot
Match: Q0J7Y8 (DEAD-box ATP-dependent RNA helicase 45 OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0154200 PE=3 SV=2)

HSP 1 Score: 84.0 bits (206), Expect = 1.7e-15
Identity = 40/72 (55.56%), Postives = 54/72 (75.00%), Query Frame = 0

Query: 65  EEVSYYRKSLAIHVSGFDVPRPIKTFEGFRFSAQLMNAIKKQGYEKPTSIQCQAMPIVLS 124
           EEV+ YRK+L + V G DVP+PIKT+     +++L++ IKK G+EKP  IQ QA+PI++S
Sbjct: 262 EEVATYRKNLELKVHGKDVPKPIKTWVQSGLTSKLLDTIKKLGFEKPMPIQAQALPIIMS 321

Query: 125 GRDIIRIAKNGS 137
           GRD I IAK GS
Sbjct: 322 GRDCIGIAKTGS 333

BLAST of Bhi08G000287 vs. ExPASy Swiss-Prot
Match: Q8H0U8 (DEAD-box ATP-dependent RNA helicase 42 OS=Arabidopsis thaliana OX=3702 GN=RH42 PE=1 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 1.4e-14
Identity = 37/75 (49.33%), Postives = 54/75 (72.00%), Query Frame = 0

Query: 62  ISEEEVSYYRKSLAIHVSGFDVPRPIKTFEGFRFSAQLMNAIKKQGYEKPTSIQCQAMPI 121
           +++EEV+ YRK L + V G DVPRPIK +     ++++++ +KK  YEKP  IQ QA+PI
Sbjct: 503 MTQEEVNTYRKELELKVHGKDVPRPIKFWHQTGLTSKILDTMKKLNYEKPMPIQTQALPI 562

Query: 122 VLSGRDIIRIAKNGS 137
           ++SGRD I +AK GS
Sbjct: 563 IMSGRDCIGVAKTGS 577

BLAST of Bhi08G000287 vs. ExPASy TrEMBL
Match: A0A6J1KCD6 (DEAD-box ATP-dependent RNA helicase 24 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493042 PE=3 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 4.4e-27
Identity = 66/76 (86.84%), Postives = 68/76 (89.47%), Query Frame = 0

Query: 61  GISEEEVSYYRKSLAIHVSGFDVPRPIKTFEGFRFSAQLMNAIKKQGYEKPTSIQCQAMP 120
           G+SEEEVS YRK+LAI VSGFDVPRPIKTFE   FS QLMNAIKKQGYEKPTSIQCQAMP
Sbjct: 204 GMSEEEVSEYRKNLAIRVSGFDVPRPIKTFEDSGFSLQLMNAIKKQGYEKPTSIQCQAMP 263

Query: 121 IVLSGRDIIRIAKNGS 137
           IVLSGRDII IAK GS
Sbjct: 264 IVLSGRDIIGIAKTGS 279

BLAST of Bhi08G000287 vs. ExPASy TrEMBL
Match: A0A6J1KGL8 (DEAD-box ATP-dependent RNA helicase 24 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493042 PE=4 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 4.4e-27
Identity = 66/76 (86.84%), Postives = 68/76 (89.47%), Query Frame = 0

Query: 61  GISEEEVSYYRKSLAIHVSGFDVPRPIKTFEGFRFSAQLMNAIKKQGYEKPTSIQCQAMP 120
           G+SEEEVS YRK+LAI VSGFDVPRPIKTFE   FS QLMNAIKKQGYEKPTSIQCQAMP
Sbjct: 204 GMSEEEVSEYRKNLAIRVSGFDVPRPIKTFEDSGFSLQLMNAIKKQGYEKPTSIQCQAMP 263

Query: 121 IVLSGRDIIRIAKNGS 137
           IVLSGRDII IAK GS
Sbjct: 264 IVLSGRDIIGIAKTGS 279

BLAST of Bhi08G000287 vs. ExPASy TrEMBL
Match: A0A6J1GB10 (DEAD-box ATP-dependent RNA helicase 24 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452364 PE=3 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 4.4e-27
Identity = 66/76 (86.84%), Postives = 68/76 (89.47%), Query Frame = 0

Query: 61  GISEEEVSYYRKSLAIHVSGFDVPRPIKTFEGFRFSAQLMNAIKKQGYEKPTSIQCQAMP 120
           G+SEEEVS YRK+LAI VSGFDVPRPIKTFE   FS QLMNAIKKQGYEKPTSIQCQAMP
Sbjct: 204 GMSEEEVSEYRKNLAIRVSGFDVPRPIKTFEDSGFSPQLMNAIKKQGYEKPTSIQCQAMP 263

Query: 121 IVLSGRDIIRIAKNGS 137
           IVLSGRDII IAK GS
Sbjct: 264 IVLSGRDIIGIAKTGS 279

BLAST of Bhi08G000287 vs. ExPASy TrEMBL
Match: A0A6J1GA90 (DEAD-box ATP-dependent RNA helicase 24 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452364 PE=3 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 4.4e-27
Identity = 66/76 (86.84%), Postives = 68/76 (89.47%), Query Frame = 0

Query: 61  GISEEEVSYYRKSLAIHVSGFDVPRPIKTFEGFRFSAQLMNAIKKQGYEKPTSIQCQAMP 120
           G+SEEEVS YRK+LAI VSGFDVPRPIKTFE   FS QLMNAIKKQGYEKPTSIQCQAMP
Sbjct: 204 GMSEEEVSEYRKNLAIRVSGFDVPRPIKTFEDSGFSPQLMNAIKKQGYEKPTSIQCQAMP 263

Query: 121 IVLSGRDIIRIAKNGS 137
           IVLSGRDII IAK GS
Sbjct: 264 IVLSGRDIIGIAKTGS 279

BLAST of Bhi08G000287 vs. ExPASy TrEMBL
Match: A0A6J1DNZ8 (DEAD-box ATP-dependent RNA helicase 24 OS=Momordica charantia OX=3673 GN=LOC111022433 PE=3 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 9.9e-27
Identity = 66/76 (86.84%), Postives = 67/76 (88.16%), Query Frame = 0

Query: 61  GISEEEVSYYRKSLAIHVSGFDVPRPIKTFEGFRFSAQLMNAIKKQGYEKPTSIQCQAMP 120
           G+SEEEVS YRKSLAI VSGFDVPRPIKTFE    S QLMNAIKKQGYEKPTSIQCQAMP
Sbjct: 201 GMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDSGLSPQLMNAIKKQGYEKPTSIQCQAMP 260

Query: 121 IVLSGRDIIRIAKNGS 137
           IVLSGRDII IAK GS
Sbjct: 261 IVLSGRDIIGIAKTGS 276

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G47330.19.1e-2568.42P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G20920.11.0e-1549.33P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G20920.21.0e-1549.33P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G09620.11.9e-1445.33P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G03900.12.1e-1371.15Iron-sulphur cluster biosynthesis family protein [more]
Match NameE-valueIdentityDescription
Q10MH81.8e-2576.32DEAD-box ATP-dependent RNA helicase 24 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
O229071.3e-2368.42DEAD-box ATP-dependent RNA helicase 24 OS=Arabidopsis thaliana OX=3702 GN=RH24 P... [more]
Q84UQ14.4e-1654.67DEAD-box ATP-dependent RNA helicase 42 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q0J7Y81.7e-1555.56DEAD-box ATP-dependent RNA helicase 45 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q8H0U81.4e-1449.33DEAD-box ATP-dependent RNA helicase 42 OS=Arabidopsis thaliana OX=3702 GN=RH42 P... [more]
Match NameE-valueIdentityDescription
A0A6J1KCD64.4e-2786.84DEAD-box ATP-dependent RNA helicase 24 isoform X2 OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1KGL84.4e-2786.84DEAD-box ATP-dependent RNA helicase 24 isoform X1 OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1GB104.4e-2786.84DEAD-box ATP-dependent RNA helicase 24 isoform X1 OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1GA904.4e-2786.84DEAD-box ATP-dependent RNA helicase 24 isoform X2 OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1DNZ89.9e-2786.84DEAD-box ATP-dependent RNA helicase 24 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 57..137
e-value: 8.0E-17
score: 63.1
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 70..136
NoneNo IPR availablePANTHERPTHR47958ATP-DEPENDENT RNA HELICASE DBP3coord: 44..136
NoneNo IPR availablePANTHERPTHR47958:SF47DEAD-BOX ATP-DEPENDENT RNA HELICASE 24coord: 44..136
IPR014014RNA helicase, DEAD-box type, Q motifPROSITEPS51195Q_MOTIFcoord: 88..116
score: 11.144666

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi08M000287Bhi08M000287mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003724 RNA helicase activity
molecular_function GO:0043565 sequence-specific DNA binding