Bhi07G001408 (gene) Wax gourd (B227) v1

Overview
NameBhi07G001408
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionhigh affinity nitrate transporter 2.5
Locationchr7: 48216382 .. 48220273 (-)
RNA-Seq ExpressionBhi07G001408
SyntenyBhi07G001408
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAATATCATACCAAACTTGTACACTCCTAAATGCACATGCTCCCAAGTATTTCTCTTTAAAAAAAAAAAAAAAAAAAATCAATGAGTTGCTTTCTCCGATTAAAATCAATGACCTCATTGATAATCATATACATGTATTCATCCCTTTATACACAATCGTCTTCTTCATGTCTCCAAGTCGTTGTTTGTTTTTGTAAAGAAATTCAAACCAATTTTTAGCCTTAAAAAATTTGTGAGAGACACCGCGAATCCCAGCATTGTTTTCATCTCCCAGAACTTCTTTCTTCAATCCCACAAAAAGCCATTTCAATTCCATTTAAATCCTACAAATTCCACACTCCTTCCCAATTCAAAACCCCCTGTTAATCCGTCGAACATGGAGGTCGACGGCGGAGCCACGGCGGCGCCGCTGCCTCACCCGAGGAAATTCTCTCTCCCGGTCGACTCAGAACACAAATCCACCAAATTCAACATATTTTCCGTTGCAGCTCCCCATATGCGAACATTTCACCTTTCCTGGATTTCCTTCTGCGCTTGTTTCATTTCCAGTTTTGCAGCGGCTCCTCTGCTTCCGGTGATCCGCGACAACCTCAATTTAACCGACACCGACATTGGAAATGCCGGAATCGCCTCTGTCTCCGGCGCCGTCTTCGCTCGAATCATGATGGGTTCCGCCTGCGATTTGGTCGGACCACGTCTCGCCTCCGCTTCACTCATTCTCATAACTTCACCGGCGGTTTATTTAACCTCAATAGCCTCATCCCCTGTTTCGTTTCTCCTGGTCCGATTCTTCACCGGCTTCTCCCTCGCTACATTCGTCTCAACCCAATTCTGGATGAGCTCAATGTTCTCCTCCAACGTCGTCGGCACCGCCAACGGCGTCGCCGGCGGCTGGGGGAACTTAGGCGGCGGCGCAACACAGTTAATCATGCCATATGTTTTCGCCATCATCGAACACATTGGCGCAACGAAATTCACCGCTTGGAGAATCGCTTTCTTCATCCCAGCTCTGTTCCAAACGCTTTCAGCCTTCGCCGTTCTCCTCCTCGGCGAGGACACGCCGGACGGAAACTTCCGGCGGCTTCAAAAAACAGGGGAGAAGGCGAAAGACAAACTGTCGAAAGTGATGTACCATGGGATGACGAATTACAGGGGATGGATTTTGGCATTGACTTATGGGTATTGTTTTGGAGTGGAGTTGACTATTGATAATGTAATAGCGAATTACTTCTACGATAGGTTTAATTTGAATCTGCATACGGCGGGGATCGTGGCGGCGAGTTTTGGGTTGGCGAATCTGTTTTCGAGGCCGTACGGCGGAGTATTGTCGGATGTGGTGGCGAAGAGATATGGGATGAGAGGGAGATTGTGGGCTTTGTGGTTGGTGCAGACAATTGGAGGAGTTTTGTGTGTGATTCTTGGATTGGTTAATTCATTAAGTGCTTCCATTGCGATCATGATCGTTTTTTCAGTGTTTGTTCAAGCTGCTTGTGGACTTACTTTTGGTGTTGTTCCCTTTGTCTCTCGAAGGTACACAAATCTACAATCCCTTTCTTCTTAATAACAATGCTTTTCTAAATGTGAAATTCGATTCGGTTTTATCAATTTTGAGCTCATCAAATTGATCTATTTTTTAAAATAAGATCTATATTGTTGTCTAATAAAAAACAAAATTGATAACTGATTTTTGTTCGATTTAGTTTATTATTGATTTTTTGGTTTTCTTTTCTTTTTTTTTTTCTTCTTCCAATTTTTTATTTATATTTTAACTTTTTCTTTTTATTTTGAATGGATCATTTTTTATACTTTCAAATTAAAATTGGGTTTATGTTTCTATATATATTTTTTAAAATTTGACATTTTTGTTTTTTTTTTTTTCTTGTCATTTTTATAAATAACAAACATTATATACACAGCAATAATTATACATACCCCATAATAATTGGTCTTTTATCATGCATGTACAATAATACTTAAAATACAAAAAGGCAATTTTCTTTAGAAGAAAATACTAAAAGATACCTAACTTAAAGTTTATTTTGAAGGGTTTGTATACGTTTGGATTTTGTTTGCTTTTTTCAATAAACCAAACCAATGGTTAAATTCGGTTTGATTTTTTGGTTTTGCGATTTTTTTATTGCATTCCCAATACTTATCTCCATACAACTAACTCTAATTTATACTCTTAATTAAATTCTAAACTAATAATTGTATCAATCTAAACTTTGAATTTTCATAAGTGTATCAATATAAATATTGTGATACTTATAATTTTATCTATTATGTCTCTAAACTTTCATAAGCGGATTAATTTAGACTCTCAATTATAATGTTATATTTTGAAAATTGTTCCTGAATCAATTTTAATCATCTATTTTATTAATATGACATTTGGAAATCTATATACGTGTAAGAATTAGATGCATGAATTGTTTTTATATGTAATCTTAATAAAAAATTTAAATTGATTCACTTATAACAATTTAGAAGTTTAATTAAAACAATCATATGGTTTGCACTAGATTTGATATAATTATTAGTTCAAAATTTAAATTAATACAATCTAAAGTTTAGAAGTGTATATTGATATTTTTTTAAAGAAAAACAGGCTTAAGTATTATTTTAATTTGTGAACTTTTATATTCGCTTTTTTTAGCACAATATAGAATAGGAGGATTCAAACTAGACCCTTCTTAGTCACTAAACACATTCATTGAACTATGCTCGTTTTAACAACTTTTATATTATTATTCAACTGAGGTCTAAAAGGACACTCAAAACCAGCCCATCAATTTATTATATTATAAATAAAATTAAAATAAATAAAATAAAGGGGTCCAGTTATTCAACCGGGTCTAAAACCACAACTAATTCTAATAATTACGACATTAATGTCAACAAATAAAAGCCGAGAATAAAACAAAAAAAGAAAAAGAAAAAACACACTCCATTTCAAAATTTCAAACCTATTCAAGTATACTGCCTAACACAATTCACACCTCCTAATTTTGTTGACTTAAATCATGGCTTGTATTAATATATCTATATACTTTTCCAAATATAACAATGAAAAAACAATCAAACACTTCAAGGTGGAAAAATGTTATCTAATATCGAATTTATCGAGAAAATATCAACGTGGATAGATACATAATCAATCATGAAATTACTAGGATTAACTGATATATCGATATATCAATAAATATTTGAATCCTTGGTGACAGATCATTAGGAGTAATATCGGGGATGACGGGCGGGGGTGGGAACGTGGGGTCGGTGCTGACTCAGCTGATATTCTTTAGAGGCAACAGATACACAAAAGAGAGAGGAATAACTCTAATGGGAGTGATGATAATAGCTTGCACCCTCCCAATTGTATTGATTTATTTTCCTCAATGGGGCAGTATGTTATTTGGGCCTTCCTCGAAAATTGCTTCAGAGGAAGATTATTACTTGTCTGAATGGACCTCTGAGGAGAAAGCCGAAGGCTTCCATATGGCTAGCCTTAAATTTGCTGAGAATAGTCGATCTGAAAGAGGCAAAAGCCTTAAGGGCACTGCTGATGCTGATGATTCCTCCTCCCCTCATGTTTAAATTGCAAAACAAATTTCCTTTTCGAGACGTAAAAAGATAGTTACTTTCTAGAATGTATCCAAGTATTTTTCTCGTTGAGTATTCCATCTTCATGGACAAATTTACTCGACTAGCTTCTTTAGAGGGTCCCTCCTTATATTTTCCATCCTATTTATTTACCATAAATCAAATCAGCTTAAATCATATGTTTAGTCTCTAAACTTTGAAATTAGCGTTTATTTAGAAATATCTAAAAGTGTCTAATAAATCTTGTGTCTCTAATATGTTATTGATAAATTTTATTTTATTTTAATTTAA

mRNA sequence

GTAATATCATACCAAACTTGTACACTCCTAAATGCACATGCTCCCAAGTATTTCTCTTTAAAAAAAAAAAAAAAAAAAATCAATGAGTTGCTTTCTCCGATTAAAATCAATGACCTCATTGATAATCATATACATGTATTCATCCCTTTATACACAATCGTCTTCTTCATGTCTCCAAGTCGTTGTTTGTTTTTGTAAAGAAATTCAAACCAATTTTTAGCCTTAAAAAATTTGTGAGAGACACCGCGAATCCCAGCATTGTTTTCATCTCCCAGAACTTCTTTCTTCAATCCCACAAAAAGCCATTTCAATTCCATTTAAATCCTACAAATTCCACACTCCTTCCCAATTCAAAACCCCCTGTTAATCCGTCGAACATGGAGGTCGACGGCGGAGCCACGGCGGCGCCGCTGCCTCACCCGAGGAAATTCTCTCTCCCGGTCGACTCAGAACACAAATCCACCAAATTCAACATATTTTCCGTTGCAGCTCCCCATATGCGAACATTTCACCTTTCCTGGATTTCCTTCTGCGCTTGTTTCATTTCCAGTTTTGCAGCGGCTCCTCTGCTTCCGGTGATCCGCGACAACCTCAATTTAACCGACACCGACATTGGAAATGCCGGAATCGCCTCTGTCTCCGGCGCCGTCTTCGCTCGAATCATGATGGGTTCCGCCTGCGATTTGGTCGGACCACGTCTCGCCTCCGCTTCACTCATTCTCATAACTTCACCGGCGGTTTATTTAACCTCAATAGCCTCATCCCCTGTTTCGTTTCTCCTGGTCCGATTCTTCACCGGCTTCTCCCTCGCTACATTCGTCTCAACCCAATTCTGGATGAGCTCAATGTTCTCCTCCAACGTCGTCGGCACCGCCAACGGCGTCGCCGGCGGCTGGGGGAACTTAGGCGGCGGCGCAACACAGTTAATCATGCCATATGTTTTCGCCATCATCGAACACATTGGCGCAACGAAATTCACCGCTTGGAGAATCGCTTTCTTCATCCCAGCTCTGTTCCAAACGCTTTCAGCCTTCGCCGTTCTCCTCCTCGGCGAGGACACGCCGGACGGAAACTTCCGGCGGCTTCAAAAAACAGGGGAGAAGGCGAAAGACAAACTGTCGAAAGTGATGTACCATGGGATGACGAATTACAGGGGATGGATTTTGGCATTGACTTATGGGTATTGTTTTGGAGTGGAGTTGACTATTGATAATGTAATAGCGAATTACTTCTACGATAGGTTTAATTTGAATCTGCATACGGCGGGGATCGTGGCGGCGAGTTTTGGGTTGGCGAATCTGTTTTCGAGGCCGTACGGCGGAGTATTGTCGGATGTGGTGGCGAAGAGATATGGGATGAGAGGGAGATTGTGGGCTTTGTGGTTGGTGCAGACAATTGGAGGAGTTTTGTGTGTGATTCTTGGATTGGTTAATTCATTAAGTGCTTCCATTGCGATCATGATCGTTTTTTCAGTGTTTGTTCAAGCTGCTTGTGGACTTACTTTTGGTGTTGTTCCCTTTGTCTCTCGAAGATCATTAGGAGTAATATCGGGGATGACGGGCGGGGGTGGGAACGTGGGGTCGGTGCTGACTCAGCTGATATTCTTTAGAGGCAACAGATACACAAAAGAGAGAGGAATAACTCTAATGGGAGTGATGATAATAGCTTGCACCCTCCCAATTGTATTGATTTATTTTCCTCAATGGGGCAGTATGTTATTTGGGCCTTCCTCGAAAATTGCTTCAGAGGAAGATTATTACTTGTCTGAATGGACCTCTGAGGAGAAAGCCGAAGGCTTCCATATGGCTAGCCTTAAATTTGCTGAGAATAGTCGATCTGAAAGAGGCAAAAGCCTTAAGGGCACTGCTGATGCTGATGATTCCTCCTCCCCTCATGTTTAAATTGCAAAACAAATTTCCTTTTCGAGACGTAAAAAGATAGTTACTTTCTAGAATGTATCCAAGTATTTTTCTCGTTGAGTATTCCATCTTCATGGACAAATTTACTCGACTAGCTTCTTTAGAGGGTCCCTCCTTATATTTTCCATCCTATTTATTTACCATAAATCAAATCAGCTTAAATCATATGTTTAGTCTCTAAACTTTGAAATTAGCGTTTATTTAGAAATATCTAAAAGTGTCTAATAAATCTTGTGTCTCTAATATGTTATTGATAAATTTTATTTTATTTTAATTTAA

Coding sequence (CDS)

ATGGAGGTCGACGGCGGAGCCACGGCGGCGCCGCTGCCTCACCCGAGGAAATTCTCTCTCCCGGTCGACTCAGAACACAAATCCACCAAATTCAACATATTTTCCGTTGCAGCTCCCCATATGCGAACATTTCACCTTTCCTGGATTTCCTTCTGCGCTTGTTTCATTTCCAGTTTTGCAGCGGCTCCTCTGCTTCCGGTGATCCGCGACAACCTCAATTTAACCGACACCGACATTGGAAATGCCGGAATCGCCTCTGTCTCCGGCGCCGTCTTCGCTCGAATCATGATGGGTTCCGCCTGCGATTTGGTCGGACCACGTCTCGCCTCCGCTTCACTCATTCTCATAACTTCACCGGCGGTTTATTTAACCTCAATAGCCTCATCCCCTGTTTCGTTTCTCCTGGTCCGATTCTTCACCGGCTTCTCCCTCGCTACATTCGTCTCAACCCAATTCTGGATGAGCTCAATGTTCTCCTCCAACGTCGTCGGCACCGCCAACGGCGTCGCCGGCGGCTGGGGGAACTTAGGCGGCGGCGCAACACAGTTAATCATGCCATATGTTTTCGCCATCATCGAACACATTGGCGCAACGAAATTCACCGCTTGGAGAATCGCTTTCTTCATCCCAGCTCTGTTCCAAACGCTTTCAGCCTTCGCCGTTCTCCTCCTCGGCGAGGACACGCCGGACGGAAACTTCCGGCGGCTTCAAAAAACAGGGGAGAAGGCGAAAGACAAACTGTCGAAAGTGATGTACCATGGGATGACGAATTACAGGGGATGGATTTTGGCATTGACTTATGGGTATTGTTTTGGAGTGGAGTTGACTATTGATAATGTAATAGCGAATTACTTCTACGATAGGTTTAATTTGAATCTGCATACGGCGGGGATCGTGGCGGCGAGTTTTGGGTTGGCGAATCTGTTTTCGAGGCCGTACGGCGGAGTATTGTCGGATGTGGTGGCGAAGAGATATGGGATGAGAGGGAGATTGTGGGCTTTGTGGTTGGTGCAGACAATTGGAGGAGTTTTGTGTGTGATTCTTGGATTGGTTAATTCATTAAGTGCTTCCATTGCGATCATGATCGTTTTTTCAGTGTTTGTTCAAGCTGCTTGTGGACTTACTTTTGGTGTTGTTCCCTTTGTCTCTCGAAGATCATTAGGAGTAATATCGGGGATGACGGGCGGGGGTGGGAACGTGGGGTCGGTGCTGACTCAGCTGATATTCTTTAGAGGCAACAGATACACAAAAGAGAGAGGAATAACTCTAATGGGAGTGATGATAATAGCTTGCACCCTCCCAATTGTATTGATTTATTTTCCTCAATGGGGCAGTATGTTATTTGGGCCTTCCTCGAAAATTGCTTCAGAGGAAGATTATTACTTGTCTGAATGGACCTCTGAGGAGAAAGCCGAAGGCTTCCATATGGCTAGCCTTAAATTTGCTGAGAATAGTCGATCTGAAAGAGGCAAAAGCCTTAAGGGCACTGCTGATGCTGATGATTCCTCCTCCCCTCATGTTTAA

Protein sequence

MEVDGGATAAPLPHPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPAVYLTSIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTGEKAKDKLSKVMYHGMTNYRGWILALTYGYCFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANLFSRPYGGVLSDVVAKRYGMRGRLWALWLVQTIGGVLCVILGLVNSLSASIAIMIVFSVFVQAACGLTFGVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTKERGITLMGVMIIACTLPIVLIYFPQWGSMLFGPSSKIASEEDYYLSEWTSEEKAEGFHMASLKFAENSRSERGKSLKGTADADDSSSPHV
Homology
BLAST of Bhi07G001408 vs. TAIR 10
Match: AT1G12940.1 (nitrate transporter2.5 )

HSP 1 Score: 702.6 bits (1812), Expect = 2.3e-202
Identity = 351/495 (70.91%), Postives = 414/495 (83.64%), Query Frame = 0

Query: 1   MEVD---GGATAAPLPHPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFIS 60
           MEV+   G A       PR+F+LPVD+E+K+T F +FSVA PHMR FHLSW  F  CF+S
Sbjct: 1   MEVEGKGGEAGTTTTTAPRRFALPVDAENKATTFRLFSVAKPHMRAFHLSWFQFFCCFVS 60

Query: 61  SFAAAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILIT 120
           +FAA PLLPVIR+NLNLT TDIGNAGIASVSGAVFARI+MG+ACDL GPRLASA+L L T
Sbjct: 61  TFAAPPLLPVIRENLNLTATDIGNAGIASVSGAVFARIVMGTACDLFGPRLASAALTLST 120

Query: 121 SPAVYLTSIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLG 180
           +PAVY T+   SP+ F++VRFF GFSLATFVSTQFWMSSMFS  VVG+ANG+A GWGNLG
Sbjct: 121 APAVYFTAGIKSPIGFIMVRFFAGFSLATFVSTQFWMSSMFSGPVVGSANGIAAGWGNLG 180

Query: 181 GGATQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQ 240
           GGATQLIMP VF++I ++GATKFTAWRIAFFIP LFQTLSAFAVLL G+D PDG++  + 
Sbjct: 181 GGATQLIMPIVFSLIRNMGATKFTAWRIAFFIPGLFQTLSAFAVLLFGQDLPDGDYWAMH 240

Query: 241 KTGEKAKDKLSKVMYHGMTNYRGWILALTYGYCFGVELTIDNVIANYFYDRFNLNLHTAG 300
           K+GE+ KD + KV+ +G+ NYRGWI AL YGYCFGVELTIDN+IA YF+DRF+L L TAG
Sbjct: 241 KSGEREKDDVGKVISNGIKNYRGWITALAYGYCFGVELTIDNIIAEYFFDRFHLKLQTAG 300

Query: 301 IVAASFGLANLFSRPYGGVLSDVVAKRYGMRGRLWALWLVQTIGGVLCVILGLVNSLSAS 360
           I+AASFGLAN F+RP GG+ SD +++R+GMRGRLWA W+VQT GGVLC  LG ++SL+ S
Sbjct: 301 IIAASFGLANFFARPGGGIFSDFMSRRFGMRGRLWAWWIVQTSGGVLCACLGQISSLTVS 360

Query: 361 IAIMIVFSVFVQAACGLTFGVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTK 420
           I +M+VFSVFVQAACGLTFGVVPF+SRRSLGV+SGMTG GGNVG+VLTQLIFF+G+ YT+
Sbjct: 361 IIVMLVFSVFVQAACGLTFGVVPFISRRSLGVVSGMTGAGGNVGAVLTQLIFFKGSTYTR 420

Query: 421 ERGITLMGVMIIACTLPIVLIYFPQWGSMLFGPSSKIASEEDYYLSEWTSEEKAEGFHMA 480
           E GITLMGVM IAC+LPI LIYFPQWG M  GPSSK  +EEDYYL+EW  EEK +  H+ 
Sbjct: 421 ETGITLMGVMSIACSLPICLIYFPQWGGMFCGPSSKKVTEEDYYLAEWNDEEKEKNLHIG 480

Query: 481 SLKFAENSRSERGKS 493
           S KFAE S SERG++
Sbjct: 481 SQKFAETSISERGRA 495

BLAST of Bhi07G001408 vs. TAIR 10
Match: AT1G08090.1 (nitrate transporter 2:1 )

HSP 1 Score: 585.5 bits (1508), Expect = 4.1e-167
Identity = 282/493 (57.20%), Postives = 367/493 (74.44%), Query Frame = 0

Query: 17  KFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDNLNLTD 76
           KF LPVD+EHK+T F +FS A PHMRTFHLSWISF  CF+S+FAAAPL+P+IR+NLNLT 
Sbjct: 38  KFDLPVDTEHKATVFKLFSFAKPHMRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTK 97

Query: 77  TDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPAVYLTSIASSPVSFLLV 136
            DIGNAG+ASVSG++F+R++MG+ CDL+GPR   A L+++++P V+  S  S    F+ V
Sbjct: 98  QDIGNAGVASVSGSIFSRLVMGAVCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITV 157

Query: 137 RFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYVFAIIEHIG 196
           RF  GF LATFVS Q+WMS+MF+S ++G  NG A GWGN+GGG TQL+MP V+ II   G
Sbjct: 158 RFMIGFCLATFVSCQYWMSTMFNSQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCG 217

Query: 197 ATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTGEKAKDKLSKVMYHGMT 256
           +T FTAWRIAFF+P     +    VL LG+D PDGN   L+K GE AKDK  K++++ +T
Sbjct: 218 STAFTAWRIAFFVPGWLHIIMGILVLNLGQDLPDGNRATLEKAGEVAKDKFGKILWYAVT 277

Query: 257 NYRGWILALTYGYCFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANLFSRPYGGV 316
           NYR WI  L YGY  GVEL+ DNVIA YF+DRF+L LHTAG++AA FG+AN F+RP GG 
Sbjct: 278 NYRTWIFVLLYGYSMGVELSTDNVIAEYFFDRFHLKLHTAGLIAACFGMANFFARPAGGY 337

Query: 317 LSDVVAKRYGMRGRLWALWLVQTIGGVLCVILGLVNSLSASIAIMIVFSVFVQAACGLTF 376
            SD  AK +GMRGRLW LW++QT GG+ CV LG  N+L  ++  M++FS+  QAACG TF
Sbjct: 338 ASDFAAKYFGMRGRLWTLWIIQTAGGLFCVWLGRANTLVTAVVAMVLFSMGAQAACGATF 397

Query: 377 GVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTKERGITLMGVMIIACTLPIV 436
            +VPFVSRR+LG+ISG+TG GGN GS LTQL+FF  + +T E+G+T MGVMI+ACTLP+ 
Sbjct: 398 AIVPFVSRRALGIISGLTGAGGNFGSGLTQLLFFSTSHFTTEQGLTWMGVMIVACTLPVT 457

Query: 437 LIYFPQWGSMLFGPSSK--IASEEDYYLSEWTSEEKAEGFHMASLKFAENSRSERGKSLK 496
           L++FPQWGSM   PS+     +E  YY SEW  +EK +  H  SL+FAEN++SE G+ ++
Sbjct: 458 LVHFPQWGSMFLPPSTDPVKGTEAHYYGSEWNEQEKQKNMHQGSLRFAENAKSEGGRRVR 517

Query: 497 GTADADDSSSPHV 508
             A   +++  +V
Sbjct: 518 SAATPPENTPNNV 530

BLAST of Bhi07G001408 vs. TAIR 10
Match: AT5G60770.1 (nitrate transporter 2.4 )

HSP 1 Score: 583.6 bits (1503), Expect = 1.6e-166
Identity = 286/502 (56.97%), Postives = 370/502 (73.71%), Query Frame = 0

Query: 9   AAPLPHPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVI 68
           A P     KFSLPVD+EHK+  F + S  APHMRTFHL+WISF  CFIS+FAAAPL+P+I
Sbjct: 30  AVPSDSSAKFSLPVDTEHKAKVFKLLSFEAPHMRTFHLAWISFFTCFISTFAAAPLVPII 89

Query: 69  RDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPAVYLTSIAS 128
           RDNLNLT  D+GNAG+ASVSG++F+R++MG+ CDL+GPR   A L+++++P V+  S   
Sbjct: 90  RDNLNLTRQDVGNAGVASVSGSIFSRLVMGAVCDLLGPRYGCAFLVMLSAPTVFSMSFVG 149

Query: 129 SPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYV 188
               ++ VRF  GF LATFVS Q+WMS+MF+  ++G  NG A GWGN+GGG TQL+MP V
Sbjct: 150 GAGGYITVRFMIGFCLATFVSCQYWMSTMFNGQIIGLVNGTAAGWGNMGGGVTQLLMPMV 209

Query: 189 FAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTGEKAKDKLS 248
           + II  +G+T FTAWR+AFF+P     +    VL LG+D PDGN   L+K G   KDK S
Sbjct: 210 YEIIRRLGSTSFTAWRMAFFVPGWMHIIMGILVLTLGQDLPDGNRSTLEKKGAVTKDKFS 269

Query: 249 KVMYHGMTNYRGWILALTYGYCFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANL 308
           KV+++ +TNYR W+  L YGY  GVELT DNVIA YF+DRF+L LHTAGI+AASFG+AN 
Sbjct: 270 KVLWYAITNYRTWVFVLLYGYSMGVELTTDNVIAEYFFDRFHLKLHTAGIIAASFGMANF 329

Query: 309 FSRPYGGVLSDVVAKRYGMRGRLWALWLVQTIGGVLCVILGLVNSLSASIAIMIVFSVFV 368
           F+RP GG  SD+ A+R+GMRGRLW LW++QT+GG  C+ LG   +L  ++  MI+FS+  
Sbjct: 330 FARPIGGWASDIAARRFGMRGRLWTLWIIQTLGGFFCLWLGRATTLPTAVVFMILFSLGA 389

Query: 369 QAACGLTFGVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTKERGITLMGVMI 428
           QAACG TF ++PF+SRRSLG+ISG+TG GGN GS LTQL+FF  + ++ E+G+T MGVMI
Sbjct: 390 QAACGATFAIIPFISRRSLGIISGLTGAGGNFGSGLTQLVFFSTSTFSTEQGLTWMGVMI 449

Query: 429 IACTLPIVLIYFPQWGSMLFGPSSK---IASEEDYYLSEWTSEEKAEGFHMASLKFAENS 488
           +ACTLP+ L++FPQWGSM F PS++    ++EE YY+ EWT  EK +G H  SLKFA NS
Sbjct: 450 MACTLPVTLVHFPQWGSM-FLPSTEDEVKSTEEYYYMKEWTETEKRKGMHEGSLKFAVNS 509

Query: 489 RSERGKSLKGTADADDSSSPHV 508
           RSERG+ +   A A      HV
Sbjct: 510 RSERGRRV---ASAPSPPPEHV 527

BLAST of Bhi07G001408 vs. TAIR 10
Match: AT1G08100.1 (nitrate transporter 2.2 )

HSP 1 Score: 580.9 bits (1496), Expect = 1.0e-165
Identity = 284/497 (57.14%), Postives = 371/497 (74.65%), Query Frame = 0

Query: 3   VDGGATAAPLPHPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAA 62
           VDG   + P    +K++LPVD+E K+T F +FS A PHMRTFHLSWISF  CF+S+FAAA
Sbjct: 25  VDG---SEPTNTKKKYNLPVDAEDKATVFKLFSFAKPHMRTFHLSWISFSTCFVSTFAAA 84

Query: 63  PLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPAVY 122
           PL+P+IR+NLNLT  DIGNAG+ASVSG++F+R++MG+ CDL+GPR   A L+++++P V+
Sbjct: 85  PLIPIIRENLNLTKHDIGNAGVASVSGSIFSRLVMGAVCDLLGPRYGCAFLVMLSAPTVF 144

Query: 123 LTSIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQ 182
             S  S    F+ VRF  GF LATFVS Q+WMS+MF+S ++G  NG A GWGN+GGG TQ
Sbjct: 145 SMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFNSQIIGLVNGTAAGWGNMGGGITQ 204

Query: 183 LIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTGEK 242
           L+MP V+ II   G+T FTAWRIAFF+P     +    VL LG+D P GN   ++K GE 
Sbjct: 205 LLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGILVLTLGQDLPGGNRAAMEKAGEV 264

Query: 243 AKDKLSKVMYHGMTNYRGWILALTYGYCFGVELTIDNVIANYFYDRFNLNLHTAGIVAAS 302
           AKDK  K++++ +TNYR WI  L YGY  GVEL+ DNVIA YF+DRF+L LHTAGI+AA 
Sbjct: 265 AKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEYFFDRFHLKLHTAGIIAAC 324

Query: 303 FGLANLFSRPYGGVLSDVVAKRYGMRGRLWALWLVQTIGGVLCVILGLVNSLSASIAIMI 362
           FG+AN F+RP GG  SD+ AKR+GMRGRLW LW++QT GG+ CV LG  N+L  ++  M+
Sbjct: 325 FGMANFFARPAGGWASDIAAKRFGMRGRLWTLWIIQTSGGLFCVWLGRANTLVTAVVSMV 384

Query: 363 VFSVFVQAACGLTFGVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTKERGIT 422
           +FS+  QAACG TF +VPFVSRR+LG+ISG+TG GGN GS LTQL+FF  +R+T E G+T
Sbjct: 385 LFSLGAQAACGATFAIVPFVSRRALGIISGLTGAGGNFGSGLTQLVFFSTSRFTTEEGLT 444

Query: 423 LMGVMIIACTLPIVLIYFPQWGSMLFGPSS-KIASEEDYYLSEWTSEEKAEGFHMASLKF 482
            MGVMI+ACTLP+ LI+FPQWGSM F PS+  + + E YY+ E++ EE+  G H+ S  F
Sbjct: 445 WMGVMIVACTLPVTLIHFPQWGSMFFPPSNDSVDATEHYYVGEYSKEEQQIGMHLKSKLF 504

Query: 483 AENSRSERGKSL-KGTA 498
           A+ +++E G S+ KG A
Sbjct: 505 ADGAKTEGGSSVHKGNA 518

BLAST of Bhi07G001408 vs. TAIR 10
Match: AT3G45060.1 (high affinity nitrate transporter 2.6 )

HSP 1 Score: 575.1 bits (1481), Expect = 5.6e-164
Identity = 287/492 (58.33%), Postives = 366/492 (74.39%), Query Frame = 0

Query: 17  KFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDNLNLTD 76
           KF+LPVDSEH++  F   S A PHMR FHL WISF  CFIS+FAAAPL+PVIRDNL+LT 
Sbjct: 48  KFALPVDSEHRAKVFKPLSFAKPHMRAFHLGWISFFTCFISTFAAAPLVPVIRDNLDLTK 107

Query: 77  TDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPAVYLTSIASSPVSFLLV 136
           TDIGNAG+ASVSGA+F+R+ MG+ CDL+G R  +A  +++T+PAV+  S  +   S+L V
Sbjct: 108 TDIGNAGVASVSGAIFSRLAMGAVCDLLGARYGTAFSLMLTAPAVFSMSFVADAGSYLAV 167

Query: 137 RFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYVFAIIEHIG 196
           RF  GF LATFVS Q+W S MF+  ++G  NG AGGWG++GGG TQL+MP VF +I+  G
Sbjct: 168 RFMIGFCLATFVSCQYWTSVMFTGKIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLTG 227

Query: 197 ATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTGEKAKDKLSKVMYHGMT 256
           AT FTAWR AFFIP + Q +    VL LG+D PDGN   LQK+G+ +KDK SKV +  + 
Sbjct: 228 ATPFTAWRFAFFIPGILQIVMGILVLTLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVK 287

Query: 257 NYRGWILALTYGYCFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANLFSRPYGGV 316
           NYR WIL + YG+  GVELTI+NVI+ YFYDRFNL LHTAGI+AASFG+AN F+RP+GG 
Sbjct: 288 NYRTWILFMLYGFSMGVELTINNVISGYFYDRFNLTLHTAGIIAASFGMANFFARPFGGY 347

Query: 317 LSDVVAKRYGMRGRLWALWLVQTIGGVLCVILGLVNSLSASIAIMIVFSVFVQAACGLTF 376
            SDV A+ +GMRGRLW LW++QT+G + C+ LG  +SL  +I  M++FS+  QAACG  F
Sbjct: 348 ASDVAARLFGMRGRLWILWILQTVGALFCIWLGRASSLPIAILAMMLFSMGTQAACGALF 407

Query: 377 GVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTKERGITLMGVMIIACTLPIV 436
           GV PFVSRRSLG+ISG+TG GGN GS +TQL+FF  +R++   G++LMGVM + C+LP+ 
Sbjct: 408 GVAPFVSRRSLGLISGLTGAGGNFGSGVTQLLFFSSSRFSTAEGLSLMGVMAVVCSLPVA 467

Query: 437 LIYFPQWGSMLFGPS--SKIASEEDYYLSEWTSEEKAEGFHMASLKFAENSRSERG-KSL 496
            I+FPQWGSM   PS   + + EE YY +EWT EEK+ G H  S+KFAENSRSERG K++
Sbjct: 468 FIHFPQWGSMFLRPSQDGEKSKEEHYYGAEWTEEEKSLGLHEGSIKFAENSRSERGRKAM 527

Query: 497 KGTADADDSSSP 506
                  ++ SP
Sbjct: 528 LADIPTPETGSP 539

BLAST of Bhi07G001408 vs. ExPASy Swiss-Prot
Match: Q9LPV5 (High affinity nitrate transporter 2.5 OS=Arabidopsis thaliana OX=3702 GN=NRT2.5 PE=1 SV=1)

HSP 1 Score: 702.6 bits (1812), Expect = 3.3e-201
Identity = 351/495 (70.91%), Postives = 414/495 (83.64%), Query Frame = 0

Query: 1   MEVD---GGATAAPLPHPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFIS 60
           MEV+   G A       PR+F+LPVD+E+K+T F +FSVA PHMR FHLSW  F  CF+S
Sbjct: 1   MEVEGKGGEAGTTTTTAPRRFALPVDAENKATTFRLFSVAKPHMRAFHLSWFQFFCCFVS 60

Query: 61  SFAAAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILIT 120
           +FAA PLLPVIR+NLNLT TDIGNAGIASVSGAVFARI+MG+ACDL GPRLASA+L L T
Sbjct: 61  TFAAPPLLPVIRENLNLTATDIGNAGIASVSGAVFARIVMGTACDLFGPRLASAALTLST 120

Query: 121 SPAVYLTSIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLG 180
           +PAVY T+   SP+ F++VRFF GFSLATFVSTQFWMSSMFS  VVG+ANG+A GWGNLG
Sbjct: 121 APAVYFTAGIKSPIGFIMVRFFAGFSLATFVSTQFWMSSMFSGPVVGSANGIAAGWGNLG 180

Query: 181 GGATQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQ 240
           GGATQLIMP VF++I ++GATKFTAWRIAFFIP LFQTLSAFAVLL G+D PDG++  + 
Sbjct: 181 GGATQLIMPIVFSLIRNMGATKFTAWRIAFFIPGLFQTLSAFAVLLFGQDLPDGDYWAMH 240

Query: 241 KTGEKAKDKLSKVMYHGMTNYRGWILALTYGYCFGVELTIDNVIANYFYDRFNLNLHTAG 300
           K+GE+ KD + KV+ +G+ NYRGWI AL YGYCFGVELTIDN+IA YF+DRF+L L TAG
Sbjct: 241 KSGEREKDDVGKVISNGIKNYRGWITALAYGYCFGVELTIDNIIAEYFFDRFHLKLQTAG 300

Query: 301 IVAASFGLANLFSRPYGGVLSDVVAKRYGMRGRLWALWLVQTIGGVLCVILGLVNSLSAS 360
           I+AASFGLAN F+RP GG+ SD +++R+GMRGRLWA W+VQT GGVLC  LG ++SL+ S
Sbjct: 301 IIAASFGLANFFARPGGGIFSDFMSRRFGMRGRLWAWWIVQTSGGVLCACLGQISSLTVS 360

Query: 361 IAIMIVFSVFVQAACGLTFGVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTK 420
           I +M+VFSVFVQAACGLTFGVVPF+SRRSLGV+SGMTG GGNVG+VLTQLIFF+G+ YT+
Sbjct: 361 IIVMLVFSVFVQAACGLTFGVVPFISRRSLGVVSGMTGAGGNVGAVLTQLIFFKGSTYTR 420

Query: 421 ERGITLMGVMIIACTLPIVLIYFPQWGSMLFGPSSKIASEEDYYLSEWTSEEKAEGFHMA 480
           E GITLMGVM IAC+LPI LIYFPQWG M  GPSSK  +EEDYYL+EW  EEK +  H+ 
Sbjct: 421 ETGITLMGVMSIACSLPICLIYFPQWGGMFCGPSSKKVTEEDYYLAEWNDEEKEKNLHIG 480

Query: 481 SLKFAENSRSERGKS 493
           S KFAE S SERG++
Sbjct: 481 SQKFAETSISERGRA 495

BLAST of Bhi07G001408 vs. ExPASy Swiss-Prot
Match: Q94JG1 (High-affinity nitrate transporter 2.3 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.3 PE=1 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 9.2e-188
Identity = 335/493 (67.95%), Postives = 399/493 (80.93%), Query Frame = 0

Query: 1   MEVDGGATAAPLPHPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFA 60
           MEV+G   A   P   +F +PVDS+ K+T+F +FS A PHM +FH++W SF  CF+S+FA
Sbjct: 8   MEVEGVEAAGGKP---RFRMPVDSDLKATEFWLFSFARPHMASFHMAWFSFFCCFVSTFA 67

Query: 61  AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPA 120
           A PLLP+IRD L LT TDIGNAGIASVSGAVFAR+ MG+ACDLVGPRLASASLIL+T+PA
Sbjct: 68  APPLLPLIRDTLGLTATDIGNAGIASVSGAVFARLAMGTACDLVGPRLASASLILLTTPA 127

Query: 121 VYLTSIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGA 180
           VY +SI  SP  +LLVRFFTG SLA+FVS QFWMSSMFS+  VG ANGVAGGWGNLGGGA
Sbjct: 128 VYCSSIIQSPSGYLLVRFFTGISLASFVSAQFWMSSMFSAPKVGLANGVAGGWGNLGGGA 187

Query: 181 TQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTG 240
            QL+MP V+  I  IG+T FTAWRIAFFIP L QT SA AVL  G+D P GN+ +L KTG
Sbjct: 188 VQLLMPLVYEAIHKIGSTPFTAWRIAFFIPGLMQTFSAIAVLAFGQDMPGGNYGKLHKTG 247

Query: 241 EKAKDKLSKVMYHGMTNYRGWILALTYGYCFGVELTIDNVIANYFYDRFNLNLHTAGIVA 300
           +  KD    V+ H +TNYRGWILALTYGY FGVELTIDNV+  YFYDRF++NL TAG++A
Sbjct: 248 DMHKDSFGNVLRHALTNYRGWILALTYGYSFGVELTIDNVVHQYFYDRFDVNLQTAGLIA 307

Query: 301 ASFGLANLFSRPYGGVLSDVVAKRYGMRGRLWALWLVQTIGGVLCVILGLVN-SLSASIA 360
           ASFG+AN+ SRP GG+LSD ++ RYGMRGRLW LW VQTIGGVLCV+LG+V+ S +AS+A
Sbjct: 308 ASFGMANIISRPGGGLLSDWLSSRYGMRGRLWGLWTVQTIGGVLCVVLGIVDFSFAASVA 367

Query: 361 IMIVFSVFVQAACGLTFGVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTKER 420
           +M++FS FVQAACGLTFG+VPFVSRRSLG+ISGMTGGGGNVG+VLTQ IFF G +Y  E 
Sbjct: 368 VMVLFSFFVQAACGLTFGIVPFVSRRSLGLISGMTGGGGNVGAVLTQYIFFHGTKYKTET 427

Query: 421 GITLMGVMIIACTLPIVLIYFPQWGSMLFGPSSKIASEEDYYLSEWTSEEKAEGFHMASL 480
           GI  MG+MIIACTLP++LIYFPQWG ML GP  K A+ E+YY  EW+  E+ +GF+ AS+
Sbjct: 428 GIKYMGLMIIACTLPVMLIYFPQWGGMLVGP-RKGATAEEYYSREWSDHEREKGFNAASV 487

Query: 481 KFAENSRSERGKS 493
           +FAENS  E G+S
Sbjct: 488 RFAENSVREGGRS 496

BLAST of Bhi07G001408 vs. ExPASy Swiss-Prot
Match: P0DKG9 (High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 3.4e-166
Identity = 290/502 (57.77%), Postives = 375/502 (74.70%), Query Frame = 0

Query: 5   GGATAAPLPHPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPL 64
           GG  AA      KF LPVDSEHK+    + S A PHMRTFHLSWISF +CF+S+FAAAPL
Sbjct: 30  GGEDAAA---ASKFDLPVDSEHKAKTIRLLSFANPHMRTFHLSWISFFSCFVSTFAAAPL 89

Query: 65  LPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPAVYLT 124
           +P+IRDNLNLT  DIGNAG+ASVSG++F+R+ MG+ CD++GPR   A LI++ +P V+  
Sbjct: 90  VPIIRDNLNLTKADIGNAGVASVSGSIFSRLAMGAICDMLGPRYGCAFLIMLAAPTVFCM 149

Query: 125 SIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLI 184
           S+  S   ++ VRF  GFSLATFVS Q+WMS+MF+S ++G  NG+A GWGN+GGGATQLI
Sbjct: 150 SLIDSAAGYIAVRFLIGFSLATFVSCQYWMSTMFNSKIIGLVNGLAAGWGNMGGGATQLI 209

Query: 185 MPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTGEKAK 244
           MP V+ +I   GAT FTAWR+A+F+P     +    VL LG+D PDGN R LQK G+  +
Sbjct: 210 MPLVYDVIRKCGATPFTAWRLAYFVPGTLHVVMGVLVLTLGQDLPDGNLRSLQKKGDVNR 269

Query: 245 DKLSKVMYHGMTNYRGWILALTYGYCFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFG 304
           D  S+V+++ +TNYR WI  L YGY  GVELT DNVIA YFYDRF+L+L  AGI+AASFG
Sbjct: 270 DSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTDNVIAEYFYDRFDLDLRVAGIIAASFG 329

Query: 305 LANLFSRPYGGVLSDVVAKRYGMRGRLWALWLVQTIGGVLCVILGLVNSLSASIAIMIVF 364
           +AN+ +RP GG+LSD+ A+ +GMR RLW +W++QT GG  C++LG  ++L  S+  M++F
Sbjct: 330 MANIVARPTGGLLSDLGARYFGMRARLWNIWILQTAGGAFCLLLGRASTLPTSVVCMVLF 389

Query: 365 SVFVQAACGLTFGVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTKERGITLM 424
           S   QAACG  FGV+PFVSRRSLG+ISGMTG GGN G+ LTQL+FF  +RY+   G+  M
Sbjct: 390 SFCAQAACGAIFGVIPFVSRRSLGIISGMTGAGGNFGAGLTQLLFFTSSRYSTGTGLEYM 449

Query: 425 GVMIIACTLPIVLIYFPQWGSMLFGPSSKIASEEDYYLSEWTSEEKAEGFHMASLKFAEN 484
           G+MI+ACTLP+VL++FPQWGSM   P++  A EE YY SEW+ +EK++G H ASLKFAEN
Sbjct: 450 GIMIMACTLPVVLVHFPQWGSMFLPPNAG-AEEEHYYGSEWSEQEKSKGLHGASLKFAEN 509

Query: 485 SRSERGKSLKGTADADDSSSPH 507
           SRSERG+     A A  ++ P+
Sbjct: 510 SRSERGRRNVINAAAAAATPPN 527

BLAST of Bhi07G001408 vs. ExPASy Swiss-Prot
Match: P0DKH0 (High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.2 PE=1 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 3.4e-166
Identity = 290/502 (57.77%), Postives = 375/502 (74.70%), Query Frame = 0

Query: 5   GGATAAPLPHPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPL 64
           GG  AA      KF LPVDSEHK+    + S A PHMRTFHLSWISF +CF+S+FAAAPL
Sbjct: 30  GGEDAAA---ASKFDLPVDSEHKAKTIRLLSFANPHMRTFHLSWISFFSCFVSTFAAAPL 89

Query: 65  LPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPAVYLT 124
           +P+IRDNLNLT  DIGNAG+ASVSG++F+R+ MG+ CD++GPR   A LI++ +P V+  
Sbjct: 90  VPIIRDNLNLTKADIGNAGVASVSGSIFSRLAMGAICDMLGPRYGCAFLIMLAAPTVFCM 149

Query: 125 SIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLI 184
           S+  S   ++ VRF  GFSLATFVS Q+WMS+MF+S ++G  NG+A GWGN+GGGATQLI
Sbjct: 150 SLIDSAAGYIAVRFLIGFSLATFVSCQYWMSTMFNSKIIGLVNGLAAGWGNMGGGATQLI 209

Query: 185 MPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTGEKAK 244
           MP V+ +I   GAT FTAWR+A+F+P     +    VL LG+D PDGN R LQK G+  +
Sbjct: 210 MPLVYDVIRKCGATPFTAWRLAYFVPGTLHVVMGVLVLTLGQDLPDGNLRSLQKKGDVNR 269

Query: 245 DKLSKVMYHGMTNYRGWILALTYGYCFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFG 304
           D  S+V+++ +TNYR WI  L YGY  GVELT DNVIA YFYDRF+L+L  AGI+AASFG
Sbjct: 270 DSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTDNVIAEYFYDRFDLDLRVAGIIAASFG 329

Query: 305 LANLFSRPYGGVLSDVVAKRYGMRGRLWALWLVQTIGGVLCVILGLVNSLSASIAIMIVF 364
           +AN+ +RP GG+LSD+ A+ +GMR RLW +W++QT GG  C++LG  ++L  S+  M++F
Sbjct: 330 MANIVARPTGGLLSDLGARYFGMRARLWNIWILQTAGGAFCLLLGRASTLPTSVVCMVLF 389

Query: 365 SVFVQAACGLTFGVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTKERGITLM 424
           S   QAACG  FGV+PFVSRRSLG+ISGMTG GGN G+ LTQL+FF  +RY+   G+  M
Sbjct: 390 SFCAQAACGAIFGVIPFVSRRSLGIISGMTGAGGNFGAGLTQLLFFTSSRYSTGTGLEYM 449

Query: 425 GVMIIACTLPIVLIYFPQWGSMLFGPSSKIASEEDYYLSEWTSEEKAEGFHMASLKFAEN 484
           G+MI+ACTLP+VL++FPQWGSM   P++  A EE YY SEW+ +EK++G H ASLKFAEN
Sbjct: 450 GIMIMACTLPVVLVHFPQWGSMFLPPNAG-AEEEHYYGSEWSEQEKSKGLHGASLKFAEN 509

Query: 485 SRSERGKSLKGTADADDSSSPH 507
           SRSERG+     A A  ++ P+
Sbjct: 510 SRSERGRRNVINAAAAAATPPN 527

BLAST of Bhi07G001408 vs. ExPASy Swiss-Prot
Match: O82811 (High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana OX=3702 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 5.8e-166
Identity = 282/493 (57.20%), Postives = 367/493 (74.44%), Query Frame = 0

Query: 17  KFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDNLNLTD 76
           KF LPVD+EHK+T F +FS A PHMRTFHLSWISF  CF+S+FAAAPL+P+IR+NLNLT 
Sbjct: 38  KFDLPVDTEHKATVFKLFSFAKPHMRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTK 97

Query: 77  TDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPAVYLTSIASSPVSFLLV 136
            DIGNAG+ASVSG++F+R++MG+ CDL+GPR   A L+++++P V+  S  S    F+ V
Sbjct: 98  QDIGNAGVASVSGSIFSRLVMGAVCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITV 157

Query: 137 RFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYVFAIIEHIG 196
           RF  GF LATFVS Q+WMS+MF+S ++G  NG A GWGN+GGG TQL+MP V+ II   G
Sbjct: 158 RFMIGFCLATFVSCQYWMSTMFNSQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCG 217

Query: 197 ATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTGEKAKDKLSKVMYHGMT 256
           +T FTAWRIAFF+P     +    VL LG+D PDGN   L+K GE AKDK  K++++ +T
Sbjct: 218 STAFTAWRIAFFVPGWLHIIMGILVLNLGQDLPDGNRATLEKAGEVAKDKFGKILWYAVT 277

Query: 257 NYRGWILALTYGYCFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANLFSRPYGGV 316
           NYR WI  L YGY  GVEL+ DNVIA YF+DRF+L LHTAG++AA FG+AN F+RP GG 
Sbjct: 278 NYRTWIFVLLYGYSMGVELSTDNVIAEYFFDRFHLKLHTAGLIAACFGMANFFARPAGGY 337

Query: 317 LSDVVAKRYGMRGRLWALWLVQTIGGVLCVILGLVNSLSASIAIMIVFSVFVQAACGLTF 376
            SD  AK +GMRGRLW LW++QT GG+ CV LG  N+L  ++  M++FS+  QAACG TF
Sbjct: 338 ASDFAAKYFGMRGRLWTLWIIQTAGGLFCVWLGRANTLVTAVVAMVLFSMGAQAACGATF 397

Query: 377 GVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTKERGITLMGVMIIACTLPIV 436
            +VPFVSRR+LG+ISG+TG GGN GS LTQL+FF  + +T E+G+T MGVMI+ACTLP+ 
Sbjct: 398 AIVPFVSRRALGIISGLTGAGGNFGSGLTQLLFFSTSHFTTEQGLTWMGVMIVACTLPVT 457

Query: 437 LIYFPQWGSMLFGPSSK--IASEEDYYLSEWTSEEKAEGFHMASLKFAENSRSERGKSLK 496
           L++FPQWGSM   PS+     +E  YY SEW  +EK +  H  SL+FAEN++SE G+ ++
Sbjct: 458 LVHFPQWGSMFLPPSTDPVKGTEAHYYGSEWNEQEKQKNMHQGSLRFAENAKSEGGRRVR 517

Query: 497 GTADADDSSSPHV 508
             A   +++  +V
Sbjct: 518 SAATPPENTPNNV 530

BLAST of Bhi07G001408 vs. ExPASy TrEMBL
Match: A0A5A7SWP7 (High affinity nitrate transporter 2.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001200 PE=4 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 3.4e-262
Identity = 469/507 (92.50%), Postives = 487/507 (96.06%), Query Frame = 0

Query: 1   MEVDGGATAAPLPHPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFA 60
           ME +GGA  A  PH RKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFA
Sbjct: 1   MEDNGGAMGA--PHQRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFA 60

Query: 61  AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPA 120
           AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPR+ASASLILITSPA
Sbjct: 61  AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRIASASLILITSPA 120

Query: 121 VYLTSIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGA 180
           VYLTSI SSPV+F L RFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGA
Sbjct: 121 VYLTSIVSSPVTFFLARFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGA 180

Query: 181 TQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTG 240
           TQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNF RL+KTG
Sbjct: 181 TQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFGRLKKTG 240

Query: 241 EKAKDKLSKVMYHGMTNYRGWILALTYGYCFGVELTIDNVIANYFYDRFNLNLHTAGIVA 300
           EKAKDK SKV+Y+G+TNYRGWILALTYGYCFGVELTIDNVIANYFYDRF+LNLHTAGIVA
Sbjct: 241 EKAKDKFSKVLYNGVTNYRGWILALTYGYCFGVELTIDNVIANYFYDRFDLNLHTAGIVA 300

Query: 301 ASFGLANLFSRPYGGVLSDVVAKRYGMRGRLWALWLVQTIGGVLCVILGLVNSLSASIAI 360
           ASFGLANLFSRPYGGVLSDVVAKRYGMRGRLWALWLVQTIGG+LCV+LGLV+SLS+SIAI
Sbjct: 301 ASFGLANLFSRPYGGVLSDVVAKRYGMRGRLWALWLVQTIGGILCVVLGLVDSLSSSIAI 360

Query: 361 MIVFSVFVQAACGLTFGVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTKERG 420
           M++FSVFVQAACGLTFGVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTKE G
Sbjct: 361 MLIFSVFVQAACGLTFGVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTKETG 420

Query: 421 ITLMGVMIIACTLPIVLIYFPQWGSMLFGPSSKIASEEDYYLSEWTSEEKAEGFHMASLK 480
           ITLMGVMIIACTLPIVLIYFPQWGSML GPSSKIASEEDYYLSEW+ EEK +G+H+ SLK
Sbjct: 421 ITLMGVMIIACTLPIVLIYFPQWGSMLLGPSSKIASEEDYYLSEWSDEEKTKGYHIGSLK 480

Query: 481 FAENSRSERGKSLKGTADADDSSSPHV 508
           FAENSRSERGK LKGTADADD S  HV
Sbjct: 481 FAENSRSERGKPLKGTADADDDSPSHV 505

BLAST of Bhi07G001408 vs. ExPASy TrEMBL
Match: A0A1S3BDY8 (high affinity nitrate transporter 2.5 OS=Cucumis melo OX=3656 GN=LOC103488988 PE=4 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 3.4e-262
Identity = 469/507 (92.50%), Postives = 487/507 (96.06%), Query Frame = 0

Query: 1   MEVDGGATAAPLPHPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFA 60
           ME +GGA  A  PH RKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFA
Sbjct: 1   MEDNGGAMGA--PHQRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFA 60

Query: 61  AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPA 120
           AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPR+ASASLILITSPA
Sbjct: 61  AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRIASASLILITSPA 120

Query: 121 VYLTSIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGA 180
           VYLTSI SSPV+F L RFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGA
Sbjct: 121 VYLTSIVSSPVTFFLARFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGA 180

Query: 181 TQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTG 240
           TQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNF RL+KTG
Sbjct: 181 TQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFGRLKKTG 240

Query: 241 EKAKDKLSKVMYHGMTNYRGWILALTYGYCFGVELTIDNVIANYFYDRFNLNLHTAGIVA 300
           EKAKDK SKV+Y+G+TNYRGWILALTYGYCFGVELTIDNVIANYFYDRF+LNLHTAGIVA
Sbjct: 241 EKAKDKFSKVLYNGVTNYRGWILALTYGYCFGVELTIDNVIANYFYDRFDLNLHTAGIVA 300

Query: 301 ASFGLANLFSRPYGGVLSDVVAKRYGMRGRLWALWLVQTIGGVLCVILGLVNSLSASIAI 360
           ASFGLANLFSRPYGGVLSDVVAKRYGMRGRLWALWLVQTIGG+LCV+LGLV+SLS+SIAI
Sbjct: 301 ASFGLANLFSRPYGGVLSDVVAKRYGMRGRLWALWLVQTIGGILCVVLGLVDSLSSSIAI 360

Query: 361 MIVFSVFVQAACGLTFGVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTKERG 420
           M++FSVFVQAACGLTFGVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTKE G
Sbjct: 361 MLIFSVFVQAACGLTFGVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTKETG 420

Query: 421 ITLMGVMIIACTLPIVLIYFPQWGSMLFGPSSKIASEEDYYLSEWTSEEKAEGFHMASLK 480
           ITLMGVMIIACTLPIVLIYFPQWGSML GPSSKIASEEDYYLSEW+ EEK +G+H+ SLK
Sbjct: 421 ITLMGVMIIACTLPIVLIYFPQWGSMLLGPSSKIASEEDYYLSEWSDEEKTKGYHIGSLK 480

Query: 481 FAENSRSERGKSLKGTADADDSSSPHV 508
           FAENSRSERGK LKGTADADD S  HV
Sbjct: 481 FAENSRSERGKPLKGTADADDDSPSHV 505

BLAST of Bhi07G001408 vs. ExPASy TrEMBL
Match: S4VIV8 (High-affinity nitrate transporter NRT2.3 OS=Cucumis sativus OX=3659 GN=NRT2.3 PE=2 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 4.5e-262
Identity = 464/495 (93.74%), Postives = 483/495 (97.58%), Query Frame = 0

Query: 13  PHPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDNL 72
           PH RKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDNL
Sbjct: 5   PHQRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFAAAPLLPVIRDNL 64

Query: 73  NLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPAVYLTSIASSPVS 132
           NLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPR+ASASLILITSPAVYLTSI SSPV+
Sbjct: 65  NLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRIASASLILITSPAVYLTSIVSSPVT 124

Query: 133 FLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYVFAII 192
           F L RFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYVFAII
Sbjct: 125 FFLARFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGATQLIMPYVFAII 184

Query: 193 EHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTGEKAKDKLSKVMY 252
           EHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNF RL+KTGEKAKDK SKV+Y
Sbjct: 185 EHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFGRLKKTGEKAKDKFSKVVY 244

Query: 253 HGMTNYRGWILALTYGYCFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANLFSRP 312
           +G+TNYRGWILALTYGYCFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANLFSRP
Sbjct: 245 NGVTNYRGWILALTYGYCFGVELTIDNVIANYFYDRFNLNLHTAGIVAASFGLANLFSRP 304

Query: 313 YGGVLSDVVAKRYGMRGRLWALWLVQTIGGVLCVILGLVNSLSASIAIMIVFSVFVQAAC 372
           YGGVLSDVVAKR+GMRGRLWALWLVQTIGG+LCV+LGLV+SLS+SIA+M++FSVFVQAAC
Sbjct: 305 YGGVLSDVVAKRFGMRGRLWALWLVQTIGGILCVVLGLVDSLSSSIAVMLIFSVFVQAAC 364

Query: 373 GLTFGVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTKERGITLMGVMIIACT 432
           GLTFGVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTKERGITLMGVMIIACT
Sbjct: 365 GLTFGVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTKERGITLMGVMIIACT 424

Query: 433 LPIVLIYFPQWGSMLFGPSSKIASEEDYYLSEWTSEEKAEGFHMASLKFAENSRSERGKS 492
           LPIVLIYFPQWGSML GPSSKIASEEDYYLSEW+ EEK +G+HM SLKFAENSR+ERGK+
Sbjct: 425 LPIVLIYFPQWGSMLLGPSSKIASEEDYYLSEWSDEEKNKGYHMGSLKFAENSRNERGKT 484

Query: 493 LKGTADADDSSSPHV 508
           LKGTADADD S PHV
Sbjct: 485 LKGTADADDDSPPHV 499

BLAST of Bhi07G001408 vs. ExPASy TrEMBL
Match: A0A6J1H2D0 (high affinity nitrate transporter 2.5 OS=Cucurbita moschata OX=3662 GN=LOC111458928 PE=4 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 7.2e-252
Identity = 457/510 (89.61%), Postives = 481/510 (94.31%), Query Frame = 0

Query: 1   MEVDGGATAAPLPHPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFA 60
           M V GGA AA    PRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACF+SSFA
Sbjct: 1   MAVQGGAPAA---QPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFVSSFA 60

Query: 61  AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPA 120
           AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPA
Sbjct: 61  AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPA 120

Query: 121 VYLTSIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGA 180
           VYLTSIASSP SF LVRFFTGFSLATFVSTQFWMSSMFSS VVGTANGVAGGWGNLGGGA
Sbjct: 121 VYLTSIASSPASFFLVRFFTGFSLATFVSTQFWMSSMFSSTVVGTANGVAGGWGNLGGGA 180

Query: 181 TQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTG 240
           TQLIMPYVFAIIE IGATKFTAWRIAFFIPA+FQTLSAFAVL+LGEDTPDGNF  LQKTG
Sbjct: 181 TQLIMPYVFAIIEQIGATKFTAWRIAFFIPAIFQTLSAFAVLMLGEDTPDGNFGELQKTG 240

Query: 241 EKAKDKLSKVMYHGMTNYRGWILALTYGYCFGVELTIDNVIANYFYDRFNLNLHTAGIVA 300
           EKAKDK S V+Y+G  NYRGWILALTYGYCFGVELTIDN+IANYFYDRFNLNLHTAGI+A
Sbjct: 241 EKAKDKFSTVVYNGAKNYRGWILALTYGYCFGVELTIDNIIANYFYDRFNLNLHTAGIIA 300

Query: 301 ASFGLANLFSRPYGGVLSDVVAKRYGMRGRLWALWLVQTIGGVLCVILGLVNSLSASIAI 360
           ASFGLANLFSRPYGG+LSDVVAKRYGMRGRLWALWLVQT+GGV CVILGLV SLS SIAI
Sbjct: 301 ASFGLANLFSRPYGGILSDVVAKRYGMRGRLWALWLVQTLGGVFCVILGLVQSLSVSIAI 360

Query: 361 MIVFSVFVQAACGLTFGVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTKERG 420
           MI+FS+FVQAACGLTFGVVPF+SRRSLGVISGMTG GGNVGSVLTQLIFFRG+RY+KE+G
Sbjct: 361 MIIFSLFVQAACGLTFGVVPFISRRSLGVISGMTGAGGNVGSVLTQLIFFRGSRYSKEKG 420

Query: 421 ITLMGVMIIACTLPIVLIYFPQWGSMLFGP-SSKIASEEDYYLSEWTSEEKAEGFHMASL 480
           I+LMGVMII CTLPIVLIYFPQWGSML GP SSKIASEE+YYLSEWT EEKA+G+H+A+L
Sbjct: 421 ISLMGVMIIGCTLPIVLIYFPQWGSMLTGPASSKIASEEEYYLSEWTPEEKAKGYHLAAL 480

Query: 481 KFAENSRSERGKSL-KGTAD-ADDSSSPHV 508
           KFA+NSR+ERGKSL KGTAD A  S+SPHV
Sbjct: 481 KFADNSRTERGKSLSKGTADAASASASPHV 507

BLAST of Bhi07G001408 vs. ExPASy TrEMBL
Match: A0A6J1JZK1 (high affinity nitrate transporter 2.5 OS=Cucurbita maxima OX=3661 GN=LOC111489712 PE=4 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 1.8e-250
Identity = 453/511 (88.65%), Postives = 480/511 (93.93%), Query Frame = 0

Query: 1   MEVDGGATAAPLPHPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFISSFA 60
           MEV GGA AA    PRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACF+SSFA
Sbjct: 1   MEVQGGAPAA---QPRKFSLPVDSEHKSTKFNIFSVAAPHMRTFHLSWISFCACFVSSFA 60

Query: 61  AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPA 120
           AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPA
Sbjct: 61  AAPLLPVIRDNLNLTDTDIGNAGIASVSGAVFARIMMGSACDLVGPRLASASLILITSPA 120

Query: 121 VYLTSIASSPVSFLLVRFFTGFSLATFVSTQFWMSSMFSSNVVGTANGVAGGWGNLGGGA 180
           VYLTSIASSP SF LVRFFTGFSLATFVSTQFWMSSMFSS VVGTANGVAGGWGNLGGGA
Sbjct: 121 VYLTSIASSPASFFLVRFFTGFSLATFVSTQFWMSSMFSSTVVGTANGVAGGWGNLGGGA 180

Query: 181 TQLIMPYVFAIIEHIGATKFTAWRIAFFIPALFQTLSAFAVLLLGEDTPDGNFRRLQKTG 240
           TQLIMPYVFAIIE IGATKFTAWRIAFFIPA+FQTLSAFAVL+LGEDTPDGNF  LQKTG
Sbjct: 181 TQLIMPYVFAIIEQIGATKFTAWRIAFFIPAIFQTLSAFAVLMLGEDTPDGNFGELQKTG 240

Query: 241 EKAKDKLSKVMYHGMTNYRGWILALTYGYCFGVELTIDNVIANYFYDRFNLNLHTAGIVA 300
           EKAKDK S V+Y+G  NYRGWILALTYGYCFGVELTIDN+IANYFYDRFNLNLHTAGI+A
Sbjct: 241 EKAKDKFSTVVYNGAKNYRGWILALTYGYCFGVELTIDNIIANYFYDRFNLNLHTAGIIA 300

Query: 301 ASFGLANLFSRPYGGVLSDVVAKRYGMRGRLWALWLVQTIGGVLCVILGLVNSLSASIAI 360
           ASFGLANLFSRPYGG+LSDVVAKRYGMRGRLWALWLVQT+GGV CVILGLV SLS SIAI
Sbjct: 301 ASFGLANLFSRPYGGILSDVVAKRYGMRGRLWALWLVQTLGGVFCVILGLVQSLSVSIAI 360

Query: 361 MIVFSVFVQAACGLTFGVVPFVSRRSLGVISGMTGGGGNVGSVLTQLIFFRGNRYTKERG 420
           MI+FS+FVQAACGLTFGVVPF+SRRSLGVISGMTG GGNVGSVLTQLIFFRG+RY+KE+G
Sbjct: 361 MIIFSLFVQAACGLTFGVVPFISRRSLGVISGMTGAGGNVGSVLTQLIFFRGSRYSKEKG 420

Query: 421 ITLMGVMIIACTLPIVLIYFPQWGSMLFGP-SSKIASEEDYYLSEWTSEEKAEGFHMASL 480
           I+LMGVMII CTLPI+LIYFPQWG ML GP SSKIASEE+YYLSEWT EE+ +G+H+A+L
Sbjct: 421 ISLMGVMIIGCTLPILLIYFPQWGGMLTGPASSKIASEEEYYLSEWTPEERVKGYHLAAL 480

Query: 481 KFAENSRSERGKSL-KGTADADD--SSSPHV 508
           KFA+NSR+ERGKSL KGTADA    ++SPHV
Sbjct: 481 KFAQNSRTERGKSLSKGTADAASAAAASPHV 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G12940.12.3e-20270.91nitrate transporter2.5 [more]
AT1G08090.14.1e-16757.20nitrate transporter 2:1 [more]
AT5G60770.11.6e-16656.97nitrate transporter 2.4 [more]
AT1G08100.11.0e-16557.14nitrate transporter 2.2 [more]
AT3G45060.15.6e-16458.33high affinity nitrate transporter 2.6 [more]
Match NameE-valueIdentityDescription
Q9LPV53.3e-20170.91High affinity nitrate transporter 2.5 OS=Arabidopsis thaliana OX=3702 GN=NRT2.5 ... [more]
Q94JG19.2e-18867.95High-affinity nitrate transporter 2.3 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
P0DKG93.4e-16657.77High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
P0DKH03.4e-16657.77High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
O828115.8e-16657.20High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana OX=3702 GN=NRT2.1 ... [more]
Match NameE-valueIdentityDescription
A0A5A7SWP73.4e-26292.50High affinity nitrate transporter 2.5 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BDY83.4e-26292.50high affinity nitrate transporter 2.5 OS=Cucumis melo OX=3656 GN=LOC103488988 PE... [more]
S4VIV84.5e-26293.74High-affinity nitrate transporter NRT2.3 OS=Cucumis sativus OX=3659 GN=NRT2.3 PE... [more]
A0A6J1H2D07.2e-25289.61high affinity nitrate transporter 2.5 OS=Cucurbita moschata OX=3662 GN=LOC111458... [more]
A0A6J1JZK11.8e-25088.65high affinity nitrate transporter 2.5 OS=Cucurbita maxima OX=3661 GN=LOC11148971... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 242..445
e-value: 1.6E-26
score: 95.0
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 37..233
e-value: 3.3E-20
score: 74.3
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 38..440
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 48..404
e-value: 3.2E-21
score: 75.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 485..507
NoneNo IPR availablePANTHERPTHR23515:SF28HIGH AFFINITY NITRATE TRANSPORTER-RELATEDcoord: 11..493
IPR044772Nitrate transporterPANTHERPTHR23515HIGH-AFFINITY NITRATE TRANSPORTER 2.3coord: 11..493
IPR044772Nitrate transporterCDDcd17341MFS_NRT2_likecoord: 44..437
e-value: 1.52718E-107
score: 323.819
IPR020846Major facilitator superfamily domainPROSITEPS50850MFScoord: 43..443
score: 8.59137

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi07M001408Bhi07M001408mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071249 cellular response to nitrate
biological_process GO:0042128 nitrate assimilation
biological_process GO:0015706 nitrate transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0015112 nitrate transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity