Bhi07G000705 (gene) Wax gourd (B227) v1

Overview
NameBhi07G000705
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionCysteine Protease
Locationchr7: 28539443 .. 28541664 (+)
RNA-Seq ExpressionBhi07G000705
SyntenyBhi07G000705
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTAAATGTGCAGTTCCATGATTCTGTGAACCGCCACGAAGACGAAGCTAGAAAAAGAGACGTTGCCAAACGGCAAAACCCGAACCAAATCAACACAAAAAAGAAGCAAAAAATCAAAATGGCAAGGGCGGAATCCCTCCTTCTAACATGCGCATTTAGCCTCACCTTGCTAACATGCGCAATTCACTCAGCCACCGCCCTCCGCCGTGACCCAGGATTGCTACGCCAAGTCACCGACGGCGAGATTATCAACAATCTCCCGGCGGGGAGCGAGAGGAAGTTCGTGATGTTCATGGAGAAGTACGGGAAGAGCTATCCGACGAGGAAGGAGTATTTGCACCGCCTTGGGATTTTTGCTAAAAATTTGATTAGGGCGGCGGAGCACCAGGCGTTGGATCCGACCGCTGTGCACGGCGTGACGCAGTTCTCGGACTTGTCGGAGGAGGAGTTTGAGCGAATGTTTATGGGAGTGAGAAGCGGCGGAGCTGGAGCGGAGTTGCGGGAGATGAATCAGGCGGCGGAGATGATGGCTGAGGAGGTGGAGGGATTGCCGGAGAGATTTGATTGGCGGGAGAAGGGGGCTGTTACCGGGGTTAAGATGCAGGTAAATTTTAGAGATTAATTTTTTAATGGGCCAATTCCAACTTTAAAAAAAAAAAAAAAAAATAATAATAATAAATTGAAAAATGGGCTCCTCTGGTTTTGCTTTTTCTTAAAAGATGAAAAAAAAAACAAATATTGCTGAAAATTTGATTGTTCTATTATTTCTATTTCTATGTGATATGAGATTATTATAAAAAAAAATTGATTTTGATTTCTTAAAATCTATTTGTCATGGAAGGTAATTTTCCATTTTTAATTTTATATAAATTATGATTGTTGTACTAAATCTCCGCTTCCTTATGGACATCTATTATTGATAGGTTTATTTATCAGTTTTTCTATGTAAAAATTTCTCTTTTGTTATTATTTTTAACATTTGTGCTTTTTTAACTTTGTTTTAATTAAAAATCTATGTATTTTTTAATGTGAAAATTATAGTACGAAATGTATGAGAAAACCTTCACAAATTTGAAAACAACATCAATATTAGTTGGGGATTGGACATTCCACTGAGGCAGACAGCTCAATTAATATTGATGATTGAGCTAAGTTCTAACTTGACAAATTGGTTCAGTTTTAAAATATAAACAACAAAATAAAGAAACTTTCATAAATTAGGGTATATGTTTTATGATGGGTTGGGAATTGGCAGGGAACGTGCGGGTCATGCTGGGCTTTCAGCACGTGTGGCGCAGTAGAAGGCGCCAATTTCATAGCCACCGGAAAGCTTCTTAGCCTCAGTGAGCAACAGCTCGTCGATTGCGATCACACGGTCAGTACACTAATTTCCCCTTCCCCAATATAATCCCAATTCCAAATCTGAACCCTTCATTTCTCAGTGCGATCCAACGGACAAAACCGCCTGCAACAACGGCTGCAACGGCGGCCTAATGACCAACGCCTACAAATACCTAATCCAATCCGGCGGCCTCGAGGAAGACTCTTCATACCCTTACACCGGCCGCCGCGGTGACTGCGATTTCCAGTCCGACAAAATCGCGGTTAGGGTCTCCAATTTCACAACCATTCCCATCGACGAGGACCAGATCGCCGCCCACCTGGTCCGCCGCGGCCCGCTCGCCGTCGGCCTCAACGCGGTCTTCATGCAGACCTACATCGGCGGCGTATCCTGTCCGTTGATTTGCGGGAAGAGATTCGTCAACCACGGCGTGCTTATGGTCGGGTACGGCGATGAAGGGTTCTCGATTCTGCGGTTTCGGAAATTGCCGTACTGGATTATAAAGAATTCTTGGGGAGAACGGTGGGGCGAGCGGGGTTATTATCGCCTGTGCCGTGGCCATGGTATGTGTGGGATTAACACTATGGTTTCGGCCGTCGTTACTCGGGCCTAAATTTTCAGTAAGTTGCGTTTGGTTTTTTTCTATACTTTTTTCTATACTGTTTTAGGGGATATGTGCTCTTTGATTTCCAGTATTTTTTTTTAATAAAGAAAAAATCTAAAAATATATTAGATTTTTTAAATATTTGTAAATATGGATTAGTCAATCTTTGATTTTTTTTTTTTAATTTCCAATTTTGTCCTCTTGTCTTATTTTATTGTACAACTTTTTTTAGTCTTTTTATTTTATTTCTCTTCTTTGATTTTTGCTTCTTCCC

mRNA sequence

ATGGTAAATGTGCAGTTCCATGATTCTGTGAACCGCCACGAAGACGAAGCTAGAAAAAGAGACGTTGCCAAACGGCAAAACCCGAACCAAATCAACACAAAAAAGAAGCAAAAAATCAAAATGGCAAGGGCGGAATCCCTCCTTCTAACATGCGCATTTAGCCTCACCTTGCTAACATGCGCAATTCACTCAGCCACCGCCCTCCGCCGTGACCCAGGATTGCTACGCCAAGTCACCGACGGCGAGATTATCAACAATCTCCCGGCGGGGAGCGAGAGGAAGTTCGTGATGTTCATGGAGAAGTACGGGAAGAGCTATCCGACGAGGAAGGAGTATTTGCACCGCCTTGGGATTTTTGCTAAAAATTTGATTAGGGCGGCGGAGCACCAGGCGTTGGATCCGACCGCTGTGCACGGCGTGACGCAGTTCTCGGACTTGTCGGAGGAGGAGTTTGAGCGAATGTTTATGGGAGTGAGAAGCGGCGGAGCTGGAGCGGAGTTGCGGGAGATGAATCAGGCGGCGGAGATGATGGCTGAGGAGGTGGAGGGATTGCCGGAGAGATTTGATTGGCGGGAGAAGGGGGCTGTTACCGGGGTTAAGATGCAGGGAACGTGCGGGTCATGCTGGGCTTTCAGCACGTGTGGCGCAGTAGAAGGCGCCAATTTCATAGCCACCGGAAAGCTTCTTAGCCTCAGTGAGCAACAGCTCGTCGATTGCGATCACACGTGCGATCCAACGGACAAAACCGCCTGCAACAACGGCTGCAACGGCGGCCTAATGACCAACGCCTACAAATACCTAATCCAATCCGGCGGCCTCGAGGAAGACTCTTCATACCCTTACACCGGCCGCCGCGGTGACTGCGATTTCCAGTCCGACAAAATCGCGGTTAGGGTCTCCAATTTCACAACCATTCCCATCGACGAGGACCAGATCGCCGCCCACCTGGTCCGCCGCGGCCCGCTCGCCGTCGGCCTCAACGCGGTCTTCATGCAGACCTACATCGGCGGCGTATCCTGTCCGTTGATTTGCGGGAAGAGATTCGTCAACCACGGCGTGCTTATGGTCGGGTACGGCGATGAAGGGTTCTCGATTCTGCGGTTTCGGAAATTGCCGTACTGGATTATAAAGAATTCTTGGGGAGAACGGTGGGGCGAGCGGGGTTATTATCGCCTGTGCCGTGGCCATGGTATGTGTGGGATTAACACTATGGTTTCGGCCGTCGTTACTCGGGCCTAAATTTTCAGTAAGTTGCGTTTGGTTTTTTTCTATACTTTTTTCTATACTGTTTTAGGGGATATGTGCTCTTTGATTTCCAGTATTTTTTTTTAATAAAGAAAAAATCTAAAAATATATTAGATTTTTTAAATATTTGTAAATATGGATTAGTCAATCTTTGATTTTTTTTTTTTAATTTCCAATTTTGTCCTCTTGTCTTATTTTATTGTACAACTTTTTTTAGTCTTTTTATTTTATTTCTCTTCTTTGATTTTTGCTTCTTCCC

Coding sequence (CDS)

ATGGTAAATGTGCAGTTCCATGATTCTGTGAACCGCCACGAAGACGAAGCTAGAAAAAGAGACGTTGCCAAACGGCAAAACCCGAACCAAATCAACACAAAAAAGAAGCAAAAAATCAAAATGGCAAGGGCGGAATCCCTCCTTCTAACATGCGCATTTAGCCTCACCTTGCTAACATGCGCAATTCACTCAGCCACCGCCCTCCGCCGTGACCCAGGATTGCTACGCCAAGTCACCGACGGCGAGATTATCAACAATCTCCCGGCGGGGAGCGAGAGGAAGTTCGTGATGTTCATGGAGAAGTACGGGAAGAGCTATCCGACGAGGAAGGAGTATTTGCACCGCCTTGGGATTTTTGCTAAAAATTTGATTAGGGCGGCGGAGCACCAGGCGTTGGATCCGACCGCTGTGCACGGCGTGACGCAGTTCTCGGACTTGTCGGAGGAGGAGTTTGAGCGAATGTTTATGGGAGTGAGAAGCGGCGGAGCTGGAGCGGAGTTGCGGGAGATGAATCAGGCGGCGGAGATGATGGCTGAGGAGGTGGAGGGATTGCCGGAGAGATTTGATTGGCGGGAGAAGGGGGCTGTTACCGGGGTTAAGATGCAGGGAACGTGCGGGTCATGCTGGGCTTTCAGCACGTGTGGCGCAGTAGAAGGCGCCAATTTCATAGCCACCGGAAAGCTTCTTAGCCTCAGTGAGCAACAGCTCGTCGATTGCGATCACACGTGCGATCCAACGGACAAAACCGCCTGCAACAACGGCTGCAACGGCGGCCTAATGACCAACGCCTACAAATACCTAATCCAATCCGGCGGCCTCGAGGAAGACTCTTCATACCCTTACACCGGCCGCCGCGGTGACTGCGATTTCCAGTCCGACAAAATCGCGGTTAGGGTCTCCAATTTCACAACCATTCCCATCGACGAGGACCAGATCGCCGCCCACCTGGTCCGCCGCGGCCCGCTCGCCGTCGGCCTCAACGCGGTCTTCATGCAGACCTACATCGGCGGCGTATCCTGTCCGTTGATTTGCGGGAAGAGATTCGTCAACCACGGCGTGCTTATGGTCGGGTACGGCGATGAAGGGTTCTCGATTCTGCGGTTTCGGAAATTGCCGTACTGGATTATAAAGAATTCTTGGGGAGAACGGTGGGGCGAGCGGGGTTATTATCGCCTGTGCCGTGGCCATGGTATGTGTGGGATTAACACTATGGTTTCGGCCGTCGTTACTCGGGCCTAA

Protein sequence

MVNVQFHDSVNRHEDEARKRDVAKRQNPNQINTKKKQKIKMARAESLLLTCAFSLTLLTCAIHSATALRRDPGLLRQVTDGEIINNLPAGSERKFVMFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFMGVRSGGAGAELREMNQAAEMMAEEVEGLPERFDWREKGAVTGVKMQGTCGSCWAFSTCGAVEGANFIATGKLLSLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEEDSSYPYTGRRGDCDFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSCPLICGKRFVNHGVLMVGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRGHGMCGINTMVSAVVTRA
Homology
BLAST of Bhi07G000705 vs. TAIR 10
Match: AT3G54940.2 (Papain family cysteine protease )

HSP 1 Score: 500.4 bits (1287), Expect = 1.4e-141
Identity = 245/374 (65.51%), Postives = 290/374 (77.54%), Query Frame = 0

Query: 41  MARAESLLLTCAFSLTLLTCAIHSATALRRDPGLLRQVT-DGEII--NNLPAGSERKFVM 100
           +A+A + L+TC      +  ++   T        +RQVT D   I  N L   +E KF +
Sbjct: 2   VAKALAQLITCIILFCHVVASVEDLT--------IRQVTADNRRIRPNLLGTHTESKFRL 61

Query: 101 FMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFMG 160
           FM  YGK+Y TR+EY+HRLGIFAKN+++AAEHQ +DP+AVHGVTQFSDL+EEEF+RM+ G
Sbjct: 62  FMSDYGKNYSTREEYIHRLGIFAKNVLKAAEHQMMDPSAVHGVTQFSDLTEEEFKRMYTG 121

Query: 161 VRSGGAGAELREMNQAAEMMAEEVEGLPERFDWREKGAVTGVKMQGTCGSCWAFSTCGAV 220
           V   G     R     AE    EV+GLPE FDWREKG VT VK QG CGSCWAFST GA 
Sbjct: 122 VADVGGS---RGGTVGAEAPMVEVDGLPEDFDWREKGGVTEVKNQGACGSCWAFSTTGAA 181

Query: 221 EGANFIATGKLLSLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEEDS 280
           EGA+F++TGKLLSLSEQQLVDCD  CDP DK AC+NGC GGLMTNAY+YL+++GGLEE+ 
Sbjct: 182 EGAHFVSTGKLLSLSEQQLVDCDQACDPKDKKACDNGCGGGLMTNAYEYLMEAGGLEEER 241

Query: 281 SYPYTGRRGDCDFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYIGG 340
           SYPYTG+RG C F  +K+AVRV NFTTIP+DE+QIAA+LVR GPLAVGLNAVFMQTYIGG
Sbjct: 242 SYPYTGKRGHCKFDPEKVAVRVLNFTTIPLDENQIAANLVRHGPLAVGLNAVFMQTYIGG 301

Query: 341 VSCPLICGKRFVNHGVLMVGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRGHG 400
           VSCPLIC KR VNHGVL+VGYG +GFSILR    PYWIIKNSWG++WGE GYY+LCRGH 
Sbjct: 302 VSCPLICSKRNVNHGVLLVGYGSKGFSILRLSNKPYWIIKNSWGKKWGENGYYKLCRGHD 361

Query: 401 MCGINTMVSAVVTR 412
           +CGIN+MVSAV T+
Sbjct: 362 ICGINSMVSAVATQ 364

BLAST of Bhi07G000705 vs. TAIR 10
Match: AT4G39090.1 (Papain family cysteine protease )

HSP 1 Score: 393.3 bits (1009), Expect = 2.4e-109
Identity = 190/357 (53.22%), Postives = 249/357 (69.75%), Query Frame = 0

Query: 53  FSLTLLTCAIHSATALRRDPGLLRQVTDGEIINNLPAGSERKFVMFMEKYGKSYPTRKEY 112
           F L+    ++ S+     D  ++RQV  G     L   SE  F +F  K+GK Y + +E+
Sbjct: 11  FVLSFFIVSVSSSDVNDGDDLVIRQVVGGAEPQVLT--SEDHFSLFKRKFGKVYASNEEH 70

Query: 113 LHRLGIFAKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFMGVRSGGAGAELREMNQ 172
            +R  +F  NL RA  HQ LDP+A HGVTQFSDL+  EF +  +GVRSG      +    
Sbjct: 71  DYRFSVFKANLRRARRHQKLDPSATHGVTQFSDLTRSEFRKKHLGVRSG-----FKLPKD 130

Query: 173 AAEMMAEEVEGLPERFDWREKGAVTGVKMQGTCGSCWAFSTCGAVEGANFIATGKLLSLS 232
           A +      E LPE FDWR+ GAVT VK QG+CGSCW+FS  GA+EGANF+ATGKL+SLS
Sbjct: 131 ANKAPILPTENLPEDFDWRDHGAVTPVKNQGSCGSCWSFSATGALEGANFLATGKLVSLS 190

Query: 233 EQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEEDSSYPYTGRRG-DCDFQ 292
           EQQLVDCDH CDP +  +C++GCNGGLM +A++Y +++GGL ++  YPYTG+ G  C   
Sbjct: 191 EQQLVDCDHECDPEEADSCDSGCNGGLMNSAFEYTLKTGGLMKEEDYPYTGKDGKTCKLD 250

Query: 293 SDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSCPLICGKRFVNH 352
             KI   VSNF+ I IDE+QIAA+LV+ GPLAV +NA +MQTYIGGVSCP IC +R +NH
Sbjct: 251 KSKIVASVSNFSVISIDEEQIAANLVKNGPLAVAINAGYMQTYIGGVSCPYICTRR-LNH 310

Query: 353 GVLMVGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRGHGMCGINTMVSAV 409
           GVL+VGYG  G++  RF++ PYWIIKNSWGE WGE G+Y++C+G  +CG+++MVS V
Sbjct: 311 GVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFYKICKGRNICGVDSMVSTV 359

BLAST of Bhi07G000705 vs. TAIR 10
Match: AT2G21430.1 (Papain family cysteine protease )

HSP 1 Score: 391.3 bits (1004), Expect = 9.3e-109
Identity = 189/361 (52.35%), Postives = 256/361 (70.91%), Query Frame = 0

Query: 49  LTCAFSLTLLTCAIHSATALRRDPGLLRQVTDGEIINNLPAGSERKFVMFMEKYGKSYPT 108
           L   FS++L+   + S +    +  L+RQV D      L   SE  F +F +K+GK Y +
Sbjct: 5   LRVLFSVSLIFVFV-SVSVCGDEDVLIRQVVDETEPKVL--SSEDHFTLFKKKFGKVYGS 64

Query: 109 RKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFMGVRSGGAGAELR 168
            +E+ +R  +F  NL+RA  HQ +DP+A HGVTQFSDL+  EF R  +GV+ G      +
Sbjct: 65  IEEHYYRFSVFKANLLRAMRHQKMDPSARHGVTQFSDLTRSEFRRKHLGVKGGFKLP--K 124

Query: 169 EMNQAAEMMAEEVEGLPERFDWREKGAVTGVKMQGTCGSCWAFSTCGAVEGANFIATGKL 228
           + NQA  +     + LPE FDWR++GAVT VK QG+CGSCW+FST GA+EGA+F+ATGKL
Sbjct: 125 DANQAPIL---PTQNLPEEFDWRDRGAVTPVKNQGSCGSCWSFSTTGALEGAHFLATGKL 184

Query: 229 LSLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEEDSSYPYTGR-RGD 288
           +SLSEQQLVDCDH CDP ++ +C++GCNGGLM +A++Y +++GGL  +  YPYTG   G 
Sbjct: 185 VSLSEQQLVDCDHECDPEEEGSCDSGCNGGLMNSAFEYTLKTGGLMREKDYPYTGTDGGS 244

Query: 289 CDFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSCPLICGKR 348
           C     KI   VSNF+ + I+EDQIAA+L++ GPLAV +NA +MQTYIGGVSCP IC +R
Sbjct: 245 CKLDRSKIVASVSNFSVVSINEDQIAANLIKNGPLAVAINAAYMQTYIGGVSCPYICSRR 304

Query: 349 FVNHGVLMVGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRGHGMCGINTMVSA 408
            +NHGVL+VGYG  GFS  R ++ PYWIIKNSWGE WGE G+Y++C+G  +CG++++VS 
Sbjct: 305 -LNHGVLLVGYGSAGFSQARLKEKPYWIIKNSWGESWGENGFYKICKGRNICGVDSLVST 356

BLAST of Bhi07G000705 vs. TAIR 10
Match: AT4G16190.1 (Papain family cysteine protease )

HSP 1 Score: 379.4 bits (973), Expect = 3.7e-105
Identity = 183/336 (54.46%), Postives = 238/336 (70.83%), Query Frame = 0

Query: 75  LRQVTDGEIINNLPAGSERKFVMFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDP 134
           +RQV   E    L   +E  F +F  KY K+Y T+ E+ HR  +F  NL RA  +Q LDP
Sbjct: 36  IRQVVPEENDEQL-LNAEHHFTLFKSKYEKTYATQVEHDHRFRVFKANLRRARRNQLLDP 95

Query: 135 TAVHGVTQFSDLSEEEFERMFMGVRSGGAGAELREMNQAAEMMAEEVEGLPERFDWREKG 194
           +AVHGVTQFSDL+ +EF R F+G++    G  L    Q A ++      LP  FDWRE+G
Sbjct: 96  SAVHGVTQFSDLTPKEFRRKFLGLKR--RGFRLPTDTQTAPIL--PTSDLPTEFDWREQG 155

Query: 195 AVTGVKMQGTCGSCWAFSTCGAVEGANFIATGKLLSLSEQQLVDCDHTCDPTDKTACNNG 254
           AVT VK QG CGSCW+FS  GA+EGA+F+AT +L+SLSEQQLVDCDH CDP    +C++G
Sbjct: 156 AVTPVKNQGMCGSCWSFSAIGALEGAHFLATKELVSLSEQQLVDCDHECDPAQANSCDSG 215

Query: 255 CNGGLMTNAYKYLIQSGGLEEDSSYPYTGR-RGDCDFQSDKIAVRVSNFTTIPIDEDQIA 314
           C+GGLM NA++Y +++GGL ++  YPYTGR    C F   KI   VSNF+ +  DEDQIA
Sbjct: 216 CSGGLMNNAFEYALKAGGLMKEEDYPYTGRDHTACKFDKSKIVASVSNFSVVSSDEDQIA 275

Query: 315 AHLVRRGPLAVGLNAVFMQTYIGGVSCPLICGKRFVNHGVLMVGYGDEGFSILRFRKLPY 374
           A+LV+ GPLA+ +NA++MQTYIGGVSCP +C K   +HGVL+VG+G  G++ +R ++ PY
Sbjct: 276 ANLVQHGPLAIAINAMWMQTYIGGVSCPYVCSKS-QDHGVLLVGFGSSGYAPIRLKEKPY 335

Query: 375 WIIKNSWGERWGERGYYRLCRG-HGMCGINTMVSAV 409
           WIIKNSWG  WGE GYY++CRG H MCG++TMVS V
Sbjct: 336 WIIKNSWGAMWGEHGYYKICRGPHNMCGMDTMVSTV 365

BLAST of Bhi07G000705 vs. TAIR 10
Match: AT1G20850.1 (xylem cysteine peptidase 2 )

HSP 1 Score: 225.7 bits (574), Expect = 6.8e-59
Identity = 128/319 (40.13%), Postives = 182/319 (57.05%), Query Frame = 0

Query: 95  FVMFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERM 154
           F  ++  + K+Y T +E   R  +F  NL    E      +   G+ +F+DLS EEF++M
Sbjct: 51  FENWISNFEKAYETVEEKFLRFEVFKDNLKHIDETNKKGKSYWLGLNEFADLSHEEFKKM 110

Query: 155 FMGVRSGGAGAELREMNQAAEMMAEEVEGLPERFDWREKGAVTGVKMQGTCGSCWAFSTC 214
           ++G+++     +  E    AE    +VE +P+  DWR+KGAV  VK QG+CGSCWAFST 
Sbjct: 111 YLGLKTDIVRRD--EERSYAEFAYRDVEAVPKSVDWRKKGAVAEVKNQGSCGSCWAFSTV 170

Query: 215 GAVEGANFIATGKLLSLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLE 274
            AVEG N I TG L +LSEQ+L+DCD        T  NNGCNGGLM  A++Y++++GGL 
Sbjct: 171 AAVEGINKIVTGNLTTLSEQELIDCD--------TTYNNGCNGGLMDYAFEYIVKNGGLR 230

Query: 275 EDSSYPYTGRRGDCDFQSDKI-AVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAV--FM 334
           ++  YPY+   G C+ Q D+   V ++    +P ++++     +   PL+V ++A     
Sbjct: 231 KEEDYPYSMEEGTCEMQKDESETVTINGHQDVPTNDEKSLLKALAHQPLSVAIDASGREF 290

Query: 335 QTYIGGVSCPLICGKRFVNHGVLMVGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYR 394
           Q Y GGV     CG   ++HGV  VGYG    S        Y I+KNSWG +WGE+GY R
Sbjct: 291 QFYSGGVFDGR-CGVD-LDHGVAAVGYGSSKGS-------DYIIVKNSWGPKWGEKGYIR 350

Query: 395 LCRG----HGMCGINTMVS 407
           L R      G+CGIN M S
Sbjct: 351 LKRNTGKPEGLCGINKMAS 350

BLAST of Bhi07G000705 vs. ExPASy Swiss-Prot
Match: Q8VYS0 (Probable cysteine protease RD19D OS=Arabidopsis thaliana OX=3702 GN=RD19D PE=2 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 2.0e-140
Identity = 245/374 (65.51%), Postives = 290/374 (77.54%), Query Frame = 0

Query: 41  MARAESLLLTCAFSLTLLTCAIHSATALRRDPGLLRQVT-DGEII--NNLPAGSERKFVM 100
           +A+A + L+TC      +  ++   T        +RQVT D   I  N L   +E KF +
Sbjct: 2   VAKALAQLITCIILFCHVVASVEDLT--------IRQVTADNRRIRPNLLGTHTESKFRL 61

Query: 101 FMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFMG 160
           FM  YGK+Y TR+EY+HRLGIFAKN+++AAEHQ +DP+AVHGVTQFSDL+EEEF+RM+ G
Sbjct: 62  FMSDYGKNYSTREEYIHRLGIFAKNVLKAAEHQMMDPSAVHGVTQFSDLTEEEFKRMYTG 121

Query: 161 VRSGGAGAELREMNQAAEMMAEEVEGLPERFDWREKGAVTGVKMQGTCGSCWAFSTCGAV 220
           V   G     R     AE    EV+GLPE FDWREKG VT VK QG CGSCWAFST GA 
Sbjct: 122 VADVGGS---RGGTVGAEAPMVEVDGLPEDFDWREKGGVTEVKNQGACGSCWAFSTTGAA 181

Query: 221 EGANFIATGKLLSLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEEDS 280
           EGA+F++TGKLLSLSEQQLVDCD  CDP DK AC+NGC GGLMTNAY+YL+++GGLEE+ 
Sbjct: 182 EGAHFVSTGKLLSLSEQQLVDCDQACDPKDKKACDNGCGGGLMTNAYEYLMEAGGLEEER 241

Query: 281 SYPYTGRRGDCDFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYIGG 340
           SYPYTG+RG C F  +K+AVRV NFTTIP+DE+QIAA+LVR GPLAVGLNAVFMQTYIGG
Sbjct: 242 SYPYTGKRGHCKFDPEKVAVRVLNFTTIPLDENQIAANLVRHGPLAVGLNAVFMQTYIGG 301

Query: 341 VSCPLICGKRFVNHGVLMVGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRGHG 400
           VSCPLIC KR VNHGVL+VGYG +GFSILR    PYWIIKNSWG++WGE GYY+LCRGH 
Sbjct: 302 VSCPLICSKRNVNHGVLLVGYGSKGFSILRLSNKPYWIIKNSWGKKWGENGYYKLCRGHD 361

Query: 401 MCGINTMVSAVVTR 412
           +CGIN+MVSAV T+
Sbjct: 362 ICGINSMVSAVATQ 364

BLAST of Bhi07G000705 vs. ExPASy Swiss-Prot
Match: P25804 (Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 1.7e-110
Identity = 188/357 (52.66%), Postives = 250/357 (70.03%), Query Frame = 0

Query: 53  FSLTLLTCAIHSAT-ALRRDPGLLRQVTDGEIINNLPAGSERKFVMFMEKYGKSYPTRKE 112
           F+L L      + T     D  ++RQV D E  + L A  E  F  F  K+ KSY T++E
Sbjct: 7   FALFLFAAVATAVTDDTNNDDFIIRQVVDNEEDHLLNA--EHHFTSFKSKFSKSYATKEE 66

Query: 113 YLHRLGIFAKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFMGVRSGGAGAELREMN 172
           + +R G+F  NLI+A  HQ  DPTA HG+T+FSDL+  EF R F+G++       LR   
Sbjct: 67  HDYRFGVFKSNLIKAKLHQNRDPTAEHGITKFSDLTASEFRRQFLGLKK-----RLRLPA 126

Query: 173 QAAEMMAEEVEGLPERFDWREKGAVTGVKMQGTCGSCWAFSTCGAVEGANFIATGKLLSL 232
            A +        LPE FDWREKGAVT VK QG+CGSCWAFST GA+EGA+++ATGKL+SL
Sbjct: 127 HAQKAPILPTTNLPEDFDWREKGAVTPVKDQGSCGSCWAFSTTGALEGAHYLATGKLVSL 186

Query: 233 SEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEEDSSYPYTGRRGDCDFQ 292
           SEQQLVDCDH CDP    +C++GCNGGLM NA++YL++SGG+ ++  Y YTGR G C F 
Sbjct: 187 SEQQLVDCDHVCDPEQAGSCDSGCNGGLMNNAFEYLLESGGVVQEKDYAYTGRDGSCKFD 246

Query: 293 SDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSCPLICGKRFVNH 352
             K+   VSNF+ + +DEDQIAA+LV+ GPLAV +NA +MQTY+ GVSCP +C K  ++H
Sbjct: 247 KSKVVASVSNFSVVTLDEDQIAANLVKNGPLAVAINAAWMQTYMSGVSCPYVCAKSRLDH 306

Query: 353 GVLMVGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRGHGMCGINTMVSAV 409
           GVL+VG+G   ++ +R ++ PYWIIKNSWG+ WGE+GYY++CRG  +CG+++MVS V
Sbjct: 307 GVLLVGFGKGAYAPIRLKEKPYWIIKNSWGQNWGEQGYYKICRGRNVCGVDSMVSTV 356

BLAST of Bhi07G000705 vs. ExPASy Swiss-Prot
Match: Q10716 (Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 2.4e-109
Identity = 190/358 (53.07%), Postives = 253/358 (70.67%), Query Frame = 0

Query: 55  LTLLTCAIHSATALRRDPGLLRQVTDGEIINNLPAGSERKFVMFMEKYGKSYPTRKEYLH 114
           L+L + A  +A     DP L+RQV  G   N+L   +E  F+ F++++GKSY    E+ +
Sbjct: 9   LSLASAAAVAAAVDAEDP-LIRQVVPGGDDNDLELNAESHFLSFVQRFGKSYKDADEHAY 68

Query: 115 RLGIFAKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFMGVRSGGAGAELREMNQAA 174
           RL +F  NL RA  HQ LDP+A HGVT+FSDL+  EF R ++G+R     A LRE+ ++A
Sbjct: 69  RLSVFKDNLRRARRHQLLDPSAEHGVTKFSDLTPAEFRRTYLGLRK-SRRALLRELGESA 128

Query: 175 -EMMAEEVEGLPERFDWREKGAVTGVKMQGTCGSCWAFSTCGAVEGANFIATGKLLSLSE 234
            E      +GLP+ FDWR+ GAV  VK QG+CGSCW+FS  GA+EGA+++ATGKL  LSE
Sbjct: 129 HEAPVLPTDGLPDDFDWRDHGAVGPVKNQGSCGSCWSFSASGALEGAHYLATGKLEVLSE 188

Query: 235 QQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEEDSSYPYTGRRGDCDFQSD 294
           QQ VDCDH CD ++  +C++GCNGGLMT A+ YL ++GGLE +  YPYTG  G C F   
Sbjct: 189 QQFVDCDHECDSSEPDSCDSGCNGGLMTTAFSYLQKAGGLESEKDYPYTGSDGKCKFDKS 248

Query: 295 KIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSCPLICGKRFVNHGV 354
           KI   V NF+ + +DE QI+A+L++ GPLA+G+NA +MQTYIGGVSCP ICG R ++HGV
Sbjct: 249 KIVASVQNFSVVSVDEAQISANLIKHGPLAIGINAAYMQTYIGGVSCPYICG-RHLDHGV 308

Query: 355 LMVGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRGHGM---CGINTMVSAV 409
           L+VGYG  GF+ +R +  PYWIIKNSWGE WGE GYY++CRG  +   CG+++MVS V
Sbjct: 309 LLVGYGASGFAPIRLKDKPYWIIKNSWGENWGENGYYKICRGSNVRNKCGVDSMVSTV 363

BLAST of Bhi07G000705 vs. ExPASy Swiss-Prot
Match: P43296 (Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 3.4e-108
Identity = 190/357 (53.22%), Postives = 249/357 (69.75%), Query Frame = 0

Query: 53  FSLTLLTCAIHSATALRRDPGLLRQVTDGEIINNLPAGSERKFVMFMEKYGKSYPTRKEY 112
           F L+    ++ S+     D  ++RQV  G     L   SE  F +F  K+GK Y + +E+
Sbjct: 11  FVLSFFIVSVSSSDVNDGDDLVIRQVVGGAEPQVLT--SEDHFSLFKRKFGKVYASNEEH 70

Query: 113 LHRLGIFAKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFMGVRSGGAGAELREMNQ 172
            +R  +F  NL RA  HQ LDP+A HGVTQFSDL+  EF +  +GVRSG      +    
Sbjct: 71  DYRFSVFKANLRRARRHQKLDPSATHGVTQFSDLTRSEFRKKHLGVRSG-----FKLPKD 130

Query: 173 AAEMMAEEVEGLPERFDWREKGAVTGVKMQGTCGSCWAFSTCGAVEGANFIATGKLLSLS 232
           A +      E LPE FDWR+ GAVT VK QG+CGSCW+FS  GA+EGANF+ATGKL+SLS
Sbjct: 131 ANKAPILPTENLPEDFDWRDHGAVTPVKNQGSCGSCWSFSATGALEGANFLATGKLVSLS 190

Query: 233 EQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEEDSSYPYTGRRG-DCDFQ 292
           EQQLVDCDH CDP +  +C++GCNGGLM +A++Y +++GGL ++  YPYTG+ G  C   
Sbjct: 191 EQQLVDCDHECDPEEADSCDSGCNGGLMNSAFEYTLKTGGLMKEEDYPYTGKDGKTCKLD 250

Query: 293 SDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSCPLICGKRFVNH 352
             KI   VSNF+ I IDE+QIAA+LV+ GPLAV +NA +MQTYIGGVSCP IC +R +NH
Sbjct: 251 KSKIVASVSNFSVISIDEEQIAANLVKNGPLAVAINAGYMQTYIGGVSCPYICTRR-LNH 310

Query: 353 GVLMVGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRGHGMCGINTMVSAV 409
           GVL+VGYG  G++  RF++ PYWIIKNSWGE WGE G+Y++C+G  +CG+++MVS V
Sbjct: 311 GVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFYKICKGRNICGVDSMVSTV 359

BLAST of Bhi07G000705 vs. ExPASy Swiss-Prot
Match: P43295 (Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 SV=2)

HSP 1 Score: 391.3 bits (1004), Expect = 1.3e-107
Identity = 189/361 (52.35%), Postives = 256/361 (70.91%), Query Frame = 0

Query: 49  LTCAFSLTLLTCAIHSATALRRDPGLLRQVTDGEIINNLPAGSERKFVMFMEKYGKSYPT 108
           L   FS++L+   + S +    +  L+RQV D      L   SE  F +F +K+GK Y +
Sbjct: 5   LRVLFSVSLIFVFV-SVSVCGDEDVLIRQVVDETEPKVL--SSEDHFTLFKKKFGKVYGS 64

Query: 109 RKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFMGVRSGGAGAELR 168
            +E+ +R  +F  NL+RA  HQ +DP+A HGVTQFSDL+  EF R  +GV+ G      +
Sbjct: 65  IEEHYYRFSVFKANLLRAMRHQKMDPSARHGVTQFSDLTRSEFRRKHLGVKGGFKLP--K 124

Query: 169 EMNQAAEMMAEEVEGLPERFDWREKGAVTGVKMQGTCGSCWAFSTCGAVEGANFIATGKL 228
           + NQA  +     + LPE FDWR++GAVT VK QG+CGSCW+FST GA+EGA+F+ATGKL
Sbjct: 125 DANQAPIL---PTQNLPEEFDWRDRGAVTPVKNQGSCGSCWSFSTTGALEGAHFLATGKL 184

Query: 229 LSLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEEDSSYPYTGR-RGD 288
           +SLSEQQLVDCDH CDP ++ +C++GCNGGLM +A++Y +++GGL  +  YPYTG   G 
Sbjct: 185 VSLSEQQLVDCDHECDPEEEGSCDSGCNGGLMNSAFEYTLKTGGLMREKDYPYTGTDGGS 244

Query: 289 CDFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSCPLICGKR 348
           C     KI   VSNF+ + I+EDQIAA+L++ GPLAV +NA +MQTYIGGVSCP IC +R
Sbjct: 245 CKLDRSKIVASVSNFSVVSINEDQIAANLIKNGPLAVAINAAYMQTYIGGVSCPYICSRR 304

Query: 349 FVNHGVLMVGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRGHGMCGINTMVSA 408
            +NHGVL+VGYG  GFS  R ++ PYWIIKNSWGE WGE G+Y++C+G  +CG++++VS 
Sbjct: 305 -LNHGVLLVGYGSAGFSQARLKEKPYWIIKNSWGESWGENGFYKICKGRNICGVDSLVST 356

BLAST of Bhi07G000705 vs. ExPASy TrEMBL
Match: A0A0A0KTI9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G622590 PE=3 SV=1)

HSP 1 Score: 714.1 bits (1842), Expect = 3.3e-202
Identity = 352/412 (85.44%), Postives = 369/412 (89.56%), Query Frame = 0

Query: 1   MVNVQFHDSVNRHEDEARKRDVAKRQNPNQINTKKKQKIKMARAESLLLTCAFSLTLLTC 60
           MV VQ +DSVNRHED+ARK  +   +      T+ + K KMA A +LLL CA SL LL  
Sbjct: 1   MVIVQLYDSVNRHEDKARKAPLPTGK------TRTRSKNKMATAVTLLLACAISLALLIS 60

Query: 61  AIHSATALRRDPGLLRQVTDGEIINNLPAGSERKFVMFMEKYGKSYPTRKEYLHRLGIFA 120
           AI SATALRRDP  LRQVTDGEI NNLPAGSERKFVMFMEKYGKSYPTRKEYLHR GIF 
Sbjct: 61  AIPSATALRRDPEFLRQVTDGEIFNNLPAGSERKFVMFMEKYGKSYPTRKEYLHRFGIFV 120

Query: 121 KNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFMGVRSGGAGAELREMNQAAEMMAEE 180
           KNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFMGVR G  G  L EMNQA E+ AEE
Sbjct: 121 KNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFMGVRGGAGGEGLPEMNQAVEVTAEE 180

Query: 181 VEGLPERFDWREKGAVTGVKMQGTCGSCWAFSTCGAVEGANFIATGKLLSLSEQQLVDCD 240
           V+GLPERFDWR+KGAVT VKMQGTCGSCWAFSTCGAVEGANFIATG LL+LSEQQLVDCD
Sbjct: 181 VKGLPERFDWRDKGAVTEVKMQGTCGSCWAFSTCGAVEGANFIATGNLLNLSEQQLVDCD 240

Query: 241 HTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEEDSSYPYTGRRGDCDFQSDKIAVRVS 300
           HTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEE+SSYPYTGR G C+FQSDKIAV+VS
Sbjct: 241 HTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEEESSYPYTGRSGQCNFQSDKIAVKVS 300

Query: 301 NFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSCPLICGKRFVNHGVLMVGYGD 360
           NFTTIPIDE+QIAAHLVR GPLAVGLNAVFMQTYIGGVSCPLICGKRFVNHGVLMVGYGD
Sbjct: 301 NFTTIPIDENQIAAHLVRSGPLAVGLNAVFMQTYIGGVSCPLICGKRFVNHGVLMVGYGD 360

Query: 361 EGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRGHGMCGINTMVSAVVTRA 413
           EGFSILRFRKLPYW+IKNSWGERWGE GYYRLCRGHGMCGINTMVSAVVT+A
Sbjct: 361 EGFSILRFRKLPYWVIKNSWGERWGEHGYYRLCRGHGMCGINTMVSAVVTQA 406

BLAST of Bhi07G000705 vs. ExPASy TrEMBL
Match: A0A5D3CBQ6 (Cysteine proteinase 15A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002450 PE=3 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 5.4e-197
Identity = 336/365 (92.05%), Postives = 344/365 (94.25%), Query Frame = 0

Query: 48  LLTCAFSLTLLTCAIHSATALRRDPGLLRQVTDGEIINNLPAGSERKFVMFMEKYGKSYP 107
           LL CAFSLTLL  AI SATALR DP  LRQVTDGEI NNLPAGSERKF MFMEKYGKSYP
Sbjct: 7   LLACAFSLTLLISAIPSATALRHDPEFLRQVTDGEIFNNLPAGSERKFAMFMEKYGKSYP 66

Query: 108 TRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFMGVRSGGAGAEL 167
           TRKEYLHRLGIF KNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFMGVR G  GA L
Sbjct: 67  TRKEYLHRLGIFVKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFMGVRGGAGGAGL 126

Query: 168 REMNQAAEMMAEEVEGLPERFDWREKGAVTGVKMQGTCGSCWAFSTCGAVEGANFIATGK 227
            EMNQA E+ AEEV+GLPERFDWREKGAVTGVKMQGTCGSCWAFSTCGAVEGANFIATGK
Sbjct: 127 PEMNQAVEVSAEEVKGLPERFDWREKGAVTGVKMQGTCGSCWAFSTCGAVEGANFIATGK 186

Query: 228 LLSLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEEDSSYPYTGRRGD 287
           LL+LSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEE+SSYPYTGR G 
Sbjct: 187 LLNLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEEESSYPYTGRSGQ 246

Query: 288 CDFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSCPLICGKR 347
           C+FQSDKIAV+VSNFTTIPIDE+QIAAHLVR GPLAVGLNAVFMQTYIGGVSCPLICGKR
Sbjct: 247 CNFQSDKIAVKVSNFTTIPIDENQIAAHLVRSGPLAVGLNAVFMQTYIGGVSCPLICGKR 306

Query: 348 FVNHGVLMVGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRGHGMCGINTMVSA 407
           FVNHGVLMVGYGDEGFSILRFRKLPYWIIKNSWGERWGE GYYRLCRGHGMCGINTMVSA
Sbjct: 307 FVNHGVLMVGYGDEGFSILRFRKLPYWIIKNSWGERWGEHGYYRLCRGHGMCGINTMVSA 366

Query: 408 VVTRA 413
           VVT+A
Sbjct: 367 VVTQA 371

BLAST of Bhi07G000705 vs. ExPASy TrEMBL
Match: A0A1S3BG12 (cysteine proteinase 15A OS=Cucumis melo OX=3656 GN=LOC103489441 PE=3 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 5.4e-197
Identity = 336/365 (92.05%), Postives = 344/365 (94.25%), Query Frame = 0

Query: 48  LLTCAFSLTLLTCAIHSATALRRDPGLLRQVTDGEIINNLPAGSERKFVMFMEKYGKSYP 107
           LL CAFSLTLL  AI SATALR DP  LRQVTDGEI NNLPAGSERKF MFMEKYGKSYP
Sbjct: 7   LLACAFSLTLLISAIPSATALRHDPEFLRQVTDGEIFNNLPAGSERKFAMFMEKYGKSYP 66

Query: 108 TRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFMGVRSGGAGAEL 167
           TRKEYLHRLGIF KNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFMGVR G  GA L
Sbjct: 67  TRKEYLHRLGIFVKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFMGVRGGAGGAGL 126

Query: 168 REMNQAAEMMAEEVEGLPERFDWREKGAVTGVKMQGTCGSCWAFSTCGAVEGANFIATGK 227
            EMNQA E+ AEEV+GLPERFDWREKGAVTGVKMQGTCGSCWAFSTCGAVEGANFIATGK
Sbjct: 127 PEMNQAVEVSAEEVKGLPERFDWREKGAVTGVKMQGTCGSCWAFSTCGAVEGANFIATGK 186

Query: 228 LLSLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEEDSSYPYTGRRGD 287
           LL+LSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEE+SSYPYTGR G 
Sbjct: 187 LLNLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEEESSYPYTGRSGQ 246

Query: 288 CDFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSCPLICGKR 347
           C+FQSDKIAV+VSNFTTIPIDE+QIAAHLVR GPLAVGLNAVFMQTYIGGVSCPLICGKR
Sbjct: 247 CNFQSDKIAVKVSNFTTIPIDENQIAAHLVRSGPLAVGLNAVFMQTYIGGVSCPLICGKR 306

Query: 348 FVNHGVLMVGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRGHGMCGINTMVSA 407
           FVNHGVLMVGYGDEGFSILRFRKLPYWIIKNSWGERWGE GYYRLCRGHGMCGINTMVSA
Sbjct: 307 FVNHGVLMVGYGDEGFSILRFRKLPYWIIKNSWGERWGEHGYYRLCRGHGMCGINTMVSA 366

Query: 408 VVTRA 413
           VVT+A
Sbjct: 367 VVTQA 371

BLAST of Bhi07G000705 vs. ExPASy TrEMBL
Match: A0A6J1GXJ8 (probable cysteine protease RD19D OS=Cucurbita moschata OX=3662 GN=LOC111458402 PE=3 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 2.9e-195
Identity = 337/372 (90.59%), Postives = 348/372 (93.55%), Query Frame = 0

Query: 41  MARAESLLLTCAFSLTLLTCAIHSATALRRDPGLLRQVTDGEIINNLPAGSERKFVMFME 100
           M R+ SLLLTCAFSL LL C    ATALR+ P  LRQVTDG IIN+LPAGSERKFVMFME
Sbjct: 1   MERSVSLLLTCAFSLALLAC----ATALRQGPEFLRQVTDGGIINSLPAGSERKFVMFME 60

Query: 101 KYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFMGVRS 160
           KYGKSYPTRKEYLHRLGIFAKNL+RAAEHQALDPTAVHGVTQFSDLSEEEFE+MFMGVR 
Sbjct: 61  KYGKSYPTRKEYLHRLGIFAKNLVRAAEHQALDPTAVHGVTQFSDLSEEEFEQMFMGVRG 120

Query: 161 GGAGAELREMNQAAEMMAEEVEGLPERFDWREKGAVTGVKMQGTCGSCWAFSTCGAVEGA 220
           G  GAEL EMNQA EM A   EGLPERFDWREKGAVT VKMQGTCGSCWAFSTCGAVEGA
Sbjct: 121 GAGGAELLEMNQAEEMTA---EGLPERFDWREKGAVTAVKMQGTCGSCWAFSTCGAVEGA 180

Query: 221 NFIATGKLLSLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEEDSSYP 280
           NFIATGKLLSLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEE+SSYP
Sbjct: 181 NFIATGKLLSLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEEESSYP 240

Query: 281 YTGRRGDCDFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSC 340
           YTG RG+C+FQSDKIAVRVSNFTTIPIDEDQIAAHLV RGPLAVGLNAVFMQTYIGGVSC
Sbjct: 241 YTGHRGECNFQSDKIAVRVSNFTTIPIDEDQIAAHLVHRGPLAVGLNAVFMQTYIGGVSC 300

Query: 341 PLICGKRFVNHGVLMVGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRGHGMCG 400
           PLICGKRFVNHGVL+VGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRGHGMCG
Sbjct: 301 PLICGKRFVNHGVLLVGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRGHGMCG 360

Query: 401 INTMVSAVVTRA 413
           +NTMVSAVVT+A
Sbjct: 361 MNTMVSAVVTQA 365

BLAST of Bhi07G000705 vs. ExPASy TrEMBL
Match: A0A6J1JBC1 (probable cysteine protease RD19D OS=Cucurbita maxima OX=3661 GN=LOC111482910 PE=3 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 3.3e-194
Identity = 335/372 (90.05%), Postives = 348/372 (93.55%), Query Frame = 0

Query: 41  MARAESLLLTCAFSLTLLTCAIHSATALRRDPGLLRQVTDGEIINNLPAGSERKFVMFME 100
           M R+ SLLLTCAFSLTLL C    ATALR+ P  LRQ+TDGEIIN+LPAGSERKFVMFME
Sbjct: 1   MERSVSLLLTCAFSLTLLAC----ATALRQGPEFLRQITDGEIINSLPAGSERKFVMFME 60

Query: 101 KYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFMGVRS 160
           KYGKSYPTRKEYLHRLGIFAKNL+RAAEHQALDPTA+HGVTQFSDLSEEEFE MFMGVR 
Sbjct: 61  KYGKSYPTRKEYLHRLGIFAKNLVRAAEHQALDPTAMHGVTQFSDLSEEEFELMFMGVRG 120

Query: 161 GGAGAELREMNQAAEMMAEEVEGLPERFDWREKGAVTGVKMQGTCGSCWAFSTCGAVEGA 220
           G  GAEL EMNQA EM A   EGLPERFDWREKGAVT VKMQGTCGSCWAFSTCGAVEGA
Sbjct: 121 GVGGAELLEMNQAEEMTA---EGLPERFDWREKGAVTAVKMQGTCGSCWAFSTCGAVEGA 180

Query: 221 NFIATGKLLSLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEEDSSYP 280
           NFIATGKLLSLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEE+SSYP
Sbjct: 181 NFIATGKLLSLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEEESSYP 240

Query: 281 YTGRRGDCDFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSC 340
           YTG RG+C+FQS KIAVRVSNFTTIPIDEDQIAAHLV RGPLAVGLNAVFMQTYIGGVSC
Sbjct: 241 YTGHRGECNFQSHKIAVRVSNFTTIPIDEDQIAAHLVHRGPLAVGLNAVFMQTYIGGVSC 300

Query: 341 PLICGKRFVNHGVLMVGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRGHGMCG 400
           PLICGKRFVNHGVL+VGYGDEGFSILRFRKLPYWIIKNSWGERWGE+GYYRLCRGHGMCG
Sbjct: 301 PLICGKRFVNHGVLLVGYGDEGFSILRFRKLPYWIIKNSWGERWGEQGYYRLCRGHGMCG 360

Query: 401 INTMVSAVVTRA 413
           +NTMVSAVVT+A
Sbjct: 361 MNTMVSAVVTQA 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G54940.21.4e-14165.51Papain family cysteine protease [more]
AT4G39090.12.4e-10953.22Papain family cysteine protease [more]
AT2G21430.19.3e-10952.35Papain family cysteine protease [more]
AT4G16190.13.7e-10554.46Papain family cysteine protease [more]
AT1G20850.16.8e-5940.13xylem cysteine peptidase 2 [more]
Match NameE-valueIdentityDescription
Q8VYS02.0e-14065.51Probable cysteine protease RD19D OS=Arabidopsis thaliana OX=3702 GN=RD19D PE=2 S... [more]
P258041.7e-11052.66Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1[more]
Q107162.4e-10953.07Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1[more]
P432963.4e-10853.22Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1[more]
P432951.3e-10752.35Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A0A0KTI93.3e-20285.44Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G622590 PE=3 SV=1[more]
A0A5D3CBQ65.4e-19792.05Cysteine proteinase 15A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3BG125.4e-19792.05cysteine proteinase 15A OS=Cucumis melo OX=3656 GN=LOC103489441 PE=3 SV=1[more]
A0A6J1GXJ82.9e-19590.59probable cysteine protease RD19D OS=Cucurbita moschata OX=3662 GN=LOC111458402 P... [more]
A0A6J1JBC13.3e-19490.05probable cysteine protease RD19D OS=Cucurbita maxima OX=3661 GN=LOC111482910 PE=... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 202..217
score: 64.84
coord: 373..379
score: 75.14
coord: 351..361
score: 58.66
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 184..409
e-value: 3.2E-107
score: 372.2
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 184..408
e-value: 1.7E-70
score: 237.6
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 95..151
e-value: 8.0E-19
score: 78.5
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 95..151
e-value: 7.1E-12
score: 45.6
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 77..410
e-value: 2.7E-98
score: 331.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..36
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 8..25
NoneNo IPR availablePANTHERPTHR12411:SF783CYSTEINE PROTEASE RD19C-RELATEDcoord: 66..409
NoneNo IPR availablePANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 66..409
IPR025660Cysteine peptidase, histidine active sitePROSITEPS00639THIOL_PROTEASE_HIScoord: 349..359
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 202..213
IPR025661Cysteine peptidase, asparagine active sitePROSITEPS00640THIOL_PROTEASE_ASNcoord: 373..392
IPR039417Papain-like cysteine endopeptidaseCDDcd02248Peptidase_C1Acoord: 185..408
e-value: 8.53417E-105
score: 306.858
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 92..408

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi07M000705Bhi07M000705mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0005615 extracellular space
cellular_component GO:0005764 lysosome
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0008234 cysteine-type peptidase activity