Bhi07G000143 (gene) Wax gourd (B227) v1

Overview


Name	Bhi07G000143
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	rRNA N-glycosidase
Location	chr7: 8242754 .. 8243203 (-)
RNA-Seq Expression	Bhi07G000143
Synteny	Bhi07G000143

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTTGTTGCAGGCGATGCAAGTTTCAGTTTATCGGGTTCAACGTCCTCATCGTACAGCAAGTTCATAAAAGATTTGAGAAACACTCTTCCATCAAACACGAAAGTGTACAATATAACTCTCTTACTCGGCTCTGCTGCCGGCACAAATCGGTACACACTAATGAAACTCTCCAATTACGATGCAAAATCAATCACAATGGCTATAGATGTAACAAACGCTATATCATGGAATATCTTGTCAACACAACTTCCTACTTTTTCAATGAATCTGATGCTAAATTTGCTTCTCAATATGTATTCAAAGGAACCACAAGCATTACACTTCCATACTTTGGCAATTACGAAAGGCTTCATATCGCTGCAGGCAAAAATCACGAGAAAATTCCTCTTGGATTCCAAGCTTGTTTCATTACGACTCGGCCGTTGCTGCTGCTGCATTCCTTGTGA

mRNA sequence

ATGCTTTGTTGCAGGCGATGCAAGTTTCAGTTTATCGGGTTCAACGTCCTCATCGTACAGCAAGTTCATAAAAGATTTGAGAAACACTCTTCCATCAAACACGAAAGTGTACAATATAACTCTCTTACTCGGCTCTGCTGCCGGCACAAATCGGTACACACTAATGAAACTCTCCAATTACGATGCAAAATCAATCACAATGGCTATAGATGTAACAAACGCTATATCATGGAATATCTTGTCAACACAACTTCCTACTTTTTCAATGAATCTGATGCTAAATTTGCTTCTCAATATGTATTCAAAGGAACCACAAGCATTACACTTCCATACTTTGGCAATTACGAAAGGCTTCATATCGCTGCAGGCAAAAATCACGAGAAAATTCCTCTTGGATTCCAAGCTTGTTTCATTACGACTCGGCCGTTGCTGCTGCTGCATTCCTTGTGA

Coding sequence (CDS)

ATGCTTTGTTGCAGGCGATGCAAGTTTCAGTTTATCGGGTTCAACGTCCTCATCGTACAGCAAGTTCATAAAAGATTTGAGAAACACTCTTCCATCAAACACGAAAGTGTACAATATAACTCTCTTACTCGGCTCTGCTGCCGGCACAAATCGGTACACACTAATGAAACTCTCCAATTACGATGCAAAATCAATCACAATGGCTATAGATGTAACAAACGCTATATCATGGAATATCTTGTCAACACAACTTCCTACTTTTTCAATGAATCTGATGCTAAATTTGCTTCTCAATATGTATTCAAAGGAACCACAAGCATTACACTTCCATACTTTGGCAATTACGAAAGGCTTCATATCGCTGCAGGCAAAAATCACGAGAAAATTCCTCTTGGATTCCAAGCTTGTTTCATTACGACTCGGCCGTTGCTGCTGCTGCATTCCTTGTGA

Protein sequence

MLCCRRCKFQFIGFNVLIVQQVHKRFEKHSSIKHESVQYNSLTRLCCRHKSVHTNETLQLRCKINHNGYRCNKRYIMEYLVNTTSYFFNESDAKFASQYVFKGTTSITLPYFGNYERLHIAAGKNHEKIPLGFQACFITTRPLLLLHSL

Homology

[Close]

BLAST of Bhi07G000143 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.3e-20
Identity = 48/61 (78.69%), Postives = 52/61 (85.25%), Query Frame = 0


Query: 75  YIMEYLVNTTSYFFNESDAKFASQYVFKGTTSITLPYFGNYERLHIAAGKNHEKIPLGFQ 134
           YIM YLVN+TSYFFNESDAK ASQYVFKG+T +TLPY GNYE+L  AAGK  EKIPLGF 
Sbjct: 90  YIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQTAAGKIREKIPLGFP 149

Query: 135 A 136
           A
Sbjct: 150 A 150

[Close]

BLAST of Bhi07G000143 vs. ExPASy Swiss-Prot
Match: P84530 (Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=2)

HSP 1 Score: 98.6 bits (244), Expect = 6.3e-20
Identity = 47/61 (77.05%), Postives = 51/61 (83.61%), Query Frame = 0


Query: 75  YIMEYLVNTTSYFFNESDAKFASQYVFKGTTSITLPYFGNYERLHIAAGKNHEKIPLGFQ 134
           YIM YLVN+TSYFFNESDAK ASQYVF G+T +TLPY GNYE+L  AAGK  EKIPLGF 
Sbjct: 70  YIMGYLVNSTSYFFNESDAKLASQYVFAGSTIVTLPYSGNYEKLQTAAGKIREKIPLGFP 129

Query: 135 A 136
           A
Sbjct: 130 A 130

[Close]

BLAST of Bhi07G000143 vs. ExPASy Swiss-Prot
Match: P22851 (Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 8.3e-20
Identity = 47/61 (77.05%), Postives = 52/61 (85.25%), Query Frame = 0


Query: 75  YIMEYLVNTTSYFFNESDAKFASQYVFKGTTSITLPYFGNYERLHIAAGKNHEKIPLGFQ 134
           YIM YLVN+TSYF NESDAK ASQYVFKG+T +T+PY GNYERL  AAGK  EKIPLGF+
Sbjct: 71  YIMGYLVNSTSYFANESDAKLASQYVFKGSTLVTIPYSGNYERLQNAAGKIREKIPLGFR 130

Query: 135 A 136
           A
Sbjct: 131 A 131

[Close]

BLAST of Bhi07G000143 vs. ExPASy Swiss-Prot
Match: Q9FRX4 (Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 2.5e-16
Identity = 40/61 (65.57%), Postives = 48/61 (78.69%), Query Frame = 0


Query: 75  YIMEYLVNTTSYFFNESDAKFASQYVFKGTTSITLPYFGNYERLHIAAGKNHEKIPLGFQ 134
           YIM YLVN TSYFFNE+DA+ AS++VF+GT SITLPY GNY++L   A K  + IPLGF 
Sbjct: 96  YIMGYLVNGTSYFFNETDAQLASKFVFQGTKSITLPYSGNYQKLQSVARKERDSIPLGFM 155

Query: 135 A 136
           A
Sbjct: 156 A 156

[Close]

BLAST of Bhi07G000143 vs. ExPASy Swiss-Prot
Match: P16094 (Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=2)

HSP 1 Score: 82.8 bits (203), Expect = 3.6e-15
Identity = 42/62 (67.74%), Postives = 46/62 (74.19%), Query Frame = 0


Query: 75  YIMEYLVNTTSYFFNESDAKFASQYVFK-GTTSITLPYFGNYERLHIAAGKNHEKIPLGF 134
           YIM YL +TTSYFFNE  A+ ASQYVF+     ITLPY GNYERL IAAGK  EKIP+G 
Sbjct: 93  YIMGYLADTTSYFFNEPAAELASQYVFRDARRKITLPYSGNYERLQIAAGKPREKIPIGL 152

Query: 135 QA 136
            A
Sbjct: 153 PA 154

[Close]

BLAST of Bhi07G000143 vs. ExPASy TrEMBL
Match: B0EVM6 (rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 2.1e-18
Identity = 49/61 (80.33%), Postives = 53/61 (86.89%), Query Frame = 0


Query: 75  YIMEYLVNTTSYFFNESDAKFASQYVFKGTTSITLPYFGNYERLHIAAGKNHEKIPLGFQ 134
           YIM YLVN+TSYFFNESDAK ASQYVFKG+T +TLPY GNYERL  AAGK  EKIPLGF+
Sbjct: 92  YIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQNAAGKVREKIPLGFR 151

Query: 135 A 136
           A
Sbjct: 152 A 152

[Close]

BLAST of Bhi07G000143 vs. ExPASy TrEMBL
Match: B0EVM2 (rRNA N-glycosylase (Fragment) OS=Senna occidentalis OX=126820 PE=4 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 2.1e-18
Identity = 48/61 (78.69%), Postives = 53/61 (86.89%), Query Frame = 0


Query: 75  YIMEYLVNTTSYFFNESDAKFASQYVFKGTTSITLPYFGNYERLHIAAGKNHEKIPLGFQ 134
           Y+M YLVN+TSYFFNESDAK ASQYVFKG+T +TLPY GNYERL  AAGK  EKIPLGF+
Sbjct: 4   YVMXYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQNAAGKVREKIPLGFR 63

Query: 135 A 136
           A
Sbjct: 64  A 64

[Close]

BLAST of Bhi07G000143 vs. ExPASy TrEMBL
Match: Q00980 (rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 2.1e-18
Identity = 49/61 (80.33%), Postives = 53/61 (86.89%), Query Frame = 0


Query: 75  YIMEYLVNTTSYFFNESDAKFASQYVFKGTTSITLPYFGNYERLHIAAGKNHEKIPLGFQ 134
           YIM YLVN+TSYFFNESDAK ASQYVFKG+T +TLPY GNYERL  AAGK  EKIPLGF+
Sbjct: 92  YIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQNAAGKVREKIPLGFR 151

Query: 135 A 136
           A
Sbjct: 152 A 152

[Close]

BLAST of Bhi07G000143 vs. ExPASy TrEMBL
Match: B0EVM1 (rRNA N-glycosidase (Fragment) OS=Alocasia macrorrhizos OX=4456 PE=4 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 2.8e-18
Identity = 48/61 (78.69%), Postives = 53/61 (86.89%), Query Frame = 0


Query: 75  YIMEYLVNTTSYFFNESDAKFASQYVFKGTTSITLPYFGNYERLHIAAGKNHEKIPLGFQ 134
           Y+M YLVN+TSYFFNESDAK ASQYVFKG+T +TLPY GNYERL  AAGK  EKIPLGF+
Sbjct: 5   YVMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQNAAGKVREKIPLGFR 64

Query: 135 A 136
           A
Sbjct: 65  A 65

[Close]

BLAST of Bhi07G000143 vs. ExPASy TrEMBL
Match: B0EVM3 (rRNA N-glycosylase (Fragment) OS=Nicotiana tabacum OX=4097 PE=4 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 2.8e-18
Identity = 48/61 (78.69%), Postives = 53/61 (86.89%), Query Frame = 0


Query: 75  YIMEYLVNTTSYFFNESDAKFASQYVFKGTTSITLPYFGNYERLHIAAGKNHEKIPLGFQ 134
           Y+M YLVN+TSYFFNESDAK ASQYVFKG+T +TLPY GNYERL  AAGK  EKIPLGF+
Sbjct: 4   YVMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYERLQNAAGKVREKIPLGFR 63

Query: 135 A 136
           A
Sbjct: 64  A 64

The following BLAST results are available for this feature:

BLAST of Bhi07G000143 vs.


Match Name	E-value	Identity	Description

BLAST of Bhi07G000143 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q00465	1.3e-20	78.69	Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P84530	6.3e-20	77.05	Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=...	[more]
P22851	8.3e-20	77.05	Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
Q9FRX4	2.5e-16	65.57	Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1	[more]
P16094	3.6e-15	67.74	Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=...	[more]

BLAST of Bhi07G000143 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
B0EVM6	2.1e-18	80.33	rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1	[more]
B0EVM2	2.1e-18	78.69	rRNA N-glycosylase (Fragment) OS=Senna occidentalis OX=126820 PE=4 SV=1	[more]
Q00980	2.1e-18	80.33	rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1	[more]
B0EVM1	2.8e-18	78.69	rRNA N-glycosidase (Fragment) OS=Alocasia macrorrhizos OX=4456 PE=4 SV=1	[more]
B0EVM3	2.8e-18	78.69	rRNA N-glycosylase (Fragment) OS=Nicotiana tabacum OX=4097 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 53..138 e-value: 8.0E-10 score: 40.7
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 75..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi07M000143	Bhi07M000143	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051607	defense response to virus
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030246	carbohydrate binding
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity