Bhi07G000011 (gene) Wax gourd (B227) v1

Overview


Name	Bhi07G000011
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like
Location	chr7: 591582 .. 591773 (-)
RNA-Seq Expression	Bhi07G000011
Synteny	Bhi07G000011

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTAGCTTTGGTTACATTCGGAGAAGGGTGGCGCAATATCCACCATGCATTCGAGTATTCGGCAAGACATGGTCATGAATGGTGGCAGATTGACTTCGGTTGGTACTTCATCATGTTTCTTCAAGCAATAGGATTGGCGACTGATGTTAAATTACCCCCAGCTTCAAAAGTAGCAGTAAAACTTTCATAA

mRNA sequence

ATGGTAGCTTTGGTTACATTCGGAGAAGGGTGGCGCAATATCCACCATGCATTCGAGTATTCGGCAAGACATGGTCATGAATGGTGGCAGATTGACTTCGGTTGGTACTTCATCATGTTTCTTCAAGCAATAGGATTGGCGACTGATGTTAAATTACCCCCAGCTTCAAAAGTAGCAGTAAAACTTTCATAA

Coding sequence (CDS)

ATGGTAGCTTTGGTTACATTCGGAGAAGGGTGGCGCAATATCCACCATGCATTCGAGTATTCGGCAAGACATGGTCATGAATGGTGGCAGATTGACTTCGGTTGGTACTTCATCATGTTTCTTCAAGCAATAGGATTGGCGACTGATGTTAAATTACCCCCAGCTTCAAAAGTAGCAGTAAAACTTTCATAA

Protein sequence

MVALVTFGEGWRNIHHAFEYSARHGHEWWQIDFGWYFIMFLQAIGLATDVKLPPASKVAVKLS

Homology

[Close]

BLAST of Bhi07G000011 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 92.0 bits (227), Expect = 1.8e-19
Identity = 39/52 (75.00%), Postives = 43/52 (82.69%), Query Frame = 0


Query: 2   VALVTFGEGWRNIHHAFEYSARHGHEWWQIDFGWYFIMFLQAIGLATDVKLP 54
           VA + FGEGW N HHAFE+SARHG EWWQ+D  WY + FLQAIGLATDVKLP
Sbjct: 307 VAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLP 358

[Close]

BLAST of Bhi07G000011 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 87.4 bits (215), Expect = 4.4e-18
Identity = 38/60 (63.33%), Postives = 47/60 (78.33%), Query Frame = 0


Query: 2   VALVTFGEGWRNIHHAFEYSARHGHEWWQIDFGWYFIMFLQAIGLATDVKLPPASKVAVK 61
           VA++T GEGW N HHAFE+SARHG EWWQ+D  W  I FL+AIGLAT+VKLP  +++  K
Sbjct: 299 VAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTETQMKGK 358

[Close]

BLAST of Bhi07G000011 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 83.2 bits (204), Expect = 8.3e-17
Identity = 35/56 (62.50%), Postives = 42/56 (75.00%), Query Frame = 0


Query: 2   VALVTFGEGWRNIHHAFEYSARHGHEWWQIDFGWYFIMFLQAIGLATDVKLPPASK 58
           +++ +FGE W N HHAFE SAR G EWWQID  WY + FL+ IGLATDVKLP  S+
Sbjct: 242 LSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQ 297

[Close]

BLAST of Bhi07G000011 vs. TAIR 10
Match: AT1G06100.1 (Fatty acid desaturase family protein )

HSP 1 Score: 81.3 bits (199), Expect = 3.1e-16
Identity = 35/52 (67.31%), Postives = 41/52 (78.85%), Query Frame = 0


Query: 2   VALVTFGEGWRNIHHAFEYSARHGHEWWQIDFGWYFIMFLQAIGLATDVKLP 54
           +AL+T GE W N HHAFE SARHG EW+Q+D  WY I F QA+GLAT+VKLP
Sbjct: 236 LALLTMGESWHNNHHAFETSARHGLEWYQLDITWYLIWFFQALGLATNVKLP 287

[Close]

BLAST of Bhi07G000011 vs. TAIR 10
Match: AT1G06090.1 (Fatty acid desaturase family protein )

HSP 1 Score: 79.3 bits (194), Expect = 1.2e-15
Identity = 34/48 (70.83%), Postives = 37/48 (77.08%), Query Frame = 0


Query: 6   TFGEGWRNIHHAFEYSARHGHEWWQIDFGWYFIMFLQAIGLATDVKLP 54
           T GE W N HHAFE SARHG EW+Q+D  WY I F QA+GLATDVKLP
Sbjct: 240 TMGESWHNNHHAFEASARHGLEWYQVDLTWYLICFFQALGLATDVKLP 287

[Close]

BLAST of Bhi07G000011 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 92.0 bits (227), Expect = 2.5e-18
Identity = 39/52 (75.00%), Postives = 43/52 (82.69%), Query Frame = 0


Query: 2   VALVTFGEGWRNIHHAFEYSARHGHEWWQIDFGWYFIMFLQAIGLATDVKLP 54
           VA + FGEGW N HHAFE+SARHG EWWQ+D  WY + FLQAIGLATDVKLP
Sbjct: 307 VAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLP 358

[Close]

BLAST of Bhi07G000011 vs. ExPASy Swiss-Prot
Match: Q9LVZ3 (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 87.4 bits (215), Expect = 6.2e-17
Identity = 38/60 (63.33%), Postives = 47/60 (78.33%), Query Frame = 0


Query: 2   VALVTFGEGWRNIHHAFEYSARHGHEWWQIDFGWYFIMFLQAIGLATDVKLPPASKVAVK 61
           VA++T GEGW N HHAFE+SARHG EWWQ+D  W  I FL+AIGLAT+VKLP  +++  K
Sbjct: 299 VAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTETQMKGK 358

[Close]

BLAST of Bhi07G000011 vs. ExPASy Swiss-Prot
Match: Q9FV68 (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.4e-16
Identity = 39/52 (75.00%), Postives = 41/52 (78.85%), Query Frame = 0


Query: 2   VALVTFGEGWRNIHHAFEYSARHGHEWWQIDFGWYFIMFLQAIGLATDVKLP 54
           VAL  FGEGW N HHAFE SARHG EWW+ID  WY I  LQAIGLAT+VKLP
Sbjct: 292 VALCAFGEGWHNNHHAFEQSARHGLEWWEIDVTWYVIRTLQAIGLATNVKLP 343

[Close]

BLAST of Bhi07G000011 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.8e-16
Identity = 35/52 (67.31%), Postives = 42/52 (80.77%), Query Frame = 0


Query: 2   VALVTFGEGWRNIHHAFEYSARHGHEWWQIDFGWYFIMFLQAIGLATDVKLP 54
           +A++T GEGW N HHAFEYSARHG EWWQID  WY I  L+ +GLATD+K+P
Sbjct: 249 IAMLTSGEGWHNNHHAFEYSARHGIEWWQIDTTWYIIKLLEYLGLATDIKVP 300

[Close]

BLAST of Bhi07G000011 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.8e-16
Identity = 36/52 (69.23%), Postives = 42/52 (80.77%), Query Frame = 0


Query: 2   VALVTFGEGWRNIHHAFEYSARHGHEWWQIDFGWYFIMFLQAIGLATDVKLP 54
           +A++T GEGW N HHAFEYSARHG EWWQID  WY I  L+ +GLATDVK+P
Sbjct: 246 IAMLTSGEGWHNNHHAFEYSARHGIEWWQIDTTWYVIKLLEYLGLATDVKVP 297

[Close]

BLAST of Bhi07G000011 vs. ExPASy TrEMBL
Match: A0A6J1DQF3 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111022855 PE=3 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-21
Identity = 48/53 (90.57%), Postives = 51/53 (96.23%), Query Frame = 0


Query: 1   MVALVTFGEGWRNIHHAFEYSARHGHEWWQIDFGWYFIMFLQAIGLATDVKLP 54
           MVALVTFGEGW N HHAFEYSARHGH+WWQIDFGWYFI+FL+AIGLATDVKLP
Sbjct: 251 MVALVTFGEGWHNNHHAFEYSARHGHKWWQIDFGWYFIIFLRAIGLATDVKLP 303

[Close]

BLAST of Bhi07G000011 vs. ExPASy TrEMBL
Match: A0A6J1JX80 (delta-9 acyl-lipid desaturase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488301 PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 3.6e-20
Identity = 47/63 (74.60%), Postives = 52/63 (82.54%), Query Frame = 0


Query: 1   MVALVTFGEGWRNIHHAFEYSARHGHEWWQIDFGWYFIMFLQAIGLATDVKLPPASKVAV 60
           MVAL+TFGEGW N HHAFEYSARHGHEWW+IDFGWY I FLQAIGLAT VKLP  ++   
Sbjct: 311 MVALITFGEGWHNNHHAFEYSARHGHEWWEIDFGWYIIKFLQAIGLATCVKLPSQTQKLA 370

Query: 61  KLS 64
            L+
Sbjct: 371 ALN 373

[Close]

BLAST of Bhi07G000011 vs. ExPASy TrEMBL
Match: A0A6J1GMY0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111455910 PE=3 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 4.7e-20
Identity = 46/53 (86.79%), Postives = 47/53 (88.68%), Query Frame = 0


Query: 1   MVALVTFGEGWRNIHHAFEYSARHGHEWWQIDFGWYFIMFLQAIGLATDVKLP 54
           MVAL+TFGEGW N HHAFEYSARHGHEWWQIDFGWY I FLQ IGLAT VKLP
Sbjct: 243 MVALITFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLQVIGLATCVKLP 295

[Close]

BLAST of Bhi07G000011 vs. ExPASy TrEMBL
Match: A0A0A0KAB1 (FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G376370 PE=3 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 6.2e-20
Identity = 45/53 (84.91%), Postives = 48/53 (90.57%), Query Frame = 0


Query: 1   MVALVTFGEGWRNIHHAFEYSARHGHEWWQIDFGWYFIMFLQAIGLATDVKLP 54
           MVAL+TFGEGW N HHAFEYSARHGHEWWQID+GWY I FLQ IGLAT+VKLP
Sbjct: 255 MVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLP 307

[Close]

BLAST of Bhi07G000011 vs. ExPASy TrEMBL
Match: A0A6J1JUL6 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111488510 PE=3 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.1e-19
Identity = 45/53 (84.91%), Postives = 47/53 (88.68%), Query Frame = 0


Query: 1   MVALVTFGEGWRNIHHAFEYSARHGHEWWQIDFGWYFIMFLQAIGLATDVKLP 54
           MVAL+TFGEGW N HHAFEYSARHGHEWWQID+GWY I FLQ IGLAT VKLP
Sbjct: 252 MVALITFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLATCVKLP 304

The following BLAST results are available for this feature:

BLAST of Bhi07G000011 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15850.1	1.8e-19	75.00	fatty acid desaturase 5	[more]
AT3G15870.1	4.4e-18	63.33	Fatty acid desaturase family protein	[more]
AT1G06080.1	8.3e-17	62.50	delta 9 desaturase 1	[more]
AT1G06100.1	3.1e-16	67.31	Fatty acid desaturase family protein	[more]
AT1G06090.1	1.2e-15	70.83	Fatty acid desaturase family protein	[more]

BLAST of Bhi07G000011 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949X0	2.5e-18	75.00	Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab...	[more]
Q9LVZ3	6.2e-17	63.33	Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9FV68	1.4e-16	75.00	Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1	[more]
P0DOW2	1.8e-16	67.31	Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1	[more]
P0DOW3	1.8e-16	69.23	Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1	[more]

BLAST of Bhi07G000011 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DQF3	1.1e-21	90.57	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1JX80	3.6e-20	74.60	delta-9 acyl-lipid desaturase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488301...	[more]
A0A6J1GMY0	4.7e-20	86.79	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A0A0KAB1	6.2e-20	84.91	FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G3763...	[more]
A0A6J1JUL6	1.1e-19	84.91	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR11351:SF87	FATTY ACID DESATURASE, TYPE 1, CORE-RELATED	coord: 1..56
IPR015876	Acyl-CoA desaturase	PANTHER	PTHR11351	ACYL-COA DESATURASE	coord: 1..56

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi07M000011	Bhi07M000011	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006633	fatty acid biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009979	16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water