Bhi05G001675 (gene) Wax gourd (B227) v1

Overview
NameBhi05G001675
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionWRKY protein
Locationchr5: 60228941 .. 60231905 (+)
RNA-Seq ExpressionBhi05G001675
SyntenyBhi05G001675
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACAAACATAGTCTGCCAATCTGTCACCATGACTACTCTTTGTATTAAACCTCATCTAATTCTCTCACTCTTTCCCTTATTCAATCTCTCCGACCAAATCCCAATTCCCTTTTTTCTCTCTAATCTCAGCCCTACGATTGATCCTCATCTAACAATTTCCTACCGCCGGATTTTCTTAATCTCATCGATTCTCTGACGCTGTTCCGCCCATGGACCGCCGTGTTCGTACCAACCCATTTGTCTCCGAGCAAGAAGATCCTGAACCCACGTCCGACGACGGCTTACCGGAATCACCCTCCGACGGCAATGACTCAAAACCCGCCGCCGGTCCACCTCCCAAAAAGAGGTTAGTTTGAAATTCAATTTCATTTTCTTCTTCTTCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTTTAATTTAACTGATTTTGTTTTATTTGGGGATATTTTTTCTGATATATACAGTAGAAGGGGAGTGCAGAAGCGAGTGGTGTCCGTACCGATCAGTGACGTGGAAGGATCTAAGAGCAAAGGGGAAGCATATCCACCGTCCGATTCATGGGCATGGAGAAAATACGGCCAAAAGCCAATCAAAGGCTCTCCTTATCCCAGGTACAACCATATACAATACAAATTCAAGGACATTTTGGGGAATTTCGGATTTTTTTTGGATGATTATTAATAATCTCCATTAATTTCATTAATTGATCTGTAATTGAATAGAAAATTGCATTTATTTTGATGCATTAAGATATTAATTTGAGCTAATTTAATGGAGTAGTTGAAGATATAGTTGGCGATTTCAATTTTAGATTTTGTTGGCCGCCGGCTGACTATGGTAGGATCTGGATTAAGATTGGCACATTTTAGCACAAAGGCAGTTTTTGCTATTAAGCGCCGAATTTTTATTTTTATTTTTCGTAGTTGGGAATTGGGATCATACTCATTATCCAATCGCTCTCACAGAAGCGTGTACAACACGCACACCTTTTTTTTTTTTCCTCACTTTTTCCCTTTTTCTTTGACCTAATTATTACACTATACACCGAATTCAATTTGGGATGGCTGTATTAAAAATATACGTTTTTTCAAATAATTTTCAAATTTTAATTATTGCTTTACTTTTTTTTTCTTCTTACCTAATTATTGCTTGATTTTTAATTATTACCGAAATTGTTTTAATTTAAATGGTTTTGGAATTTTGATTAAATTTGAACAAACGGAAATTACGATATTATTCCAAGGTGCCTTTCACATCGAGGGCGTGAAGGTGATTGGCGGCCAAATGAAAGGTCTTTAATAAAATATCAATTATTGGCTTTTGGGCGGCATAGATAAATCAAATAGTCAATTTGTTTGATTCAAAAAACAACAAGAAAAAAGATAAAGGAGAAAGAAATAGTCAAATAGTTATTTTTTCCCGTTTTTTATAAAAAAAATAAACAAATTATCCATAGAGTATAAAAGTATACGAAGTTTAAAAAGGGTTATAAAGAATATGCTTTTCTTTAATTTAATAATTGGATGATAGGAAAATTAAAATGATAAATGTCATATTTACTAAATTAGGTTATTAAAATTCACATGAGTAGTTGGTAGCTAGAAAAAGAAAGCTTAAATTATAAAAATACCCCTTAATCTTTCATTTGTTTTTAAAATATACTTATTCTATTTAATATTACAATATTTCTATTGATCTTAAAACTATATTAGGGGTATAGATATTTAAAATTTTTGAATGAAAATTAAAATATAGAATGAGTGAAATACGATTATATAAGAAATTTTGTAATGTCTATCTTTAAATAATTCTATAATATATCGTTTACGAGATATCAAAGATAATATTGCAATTTAAAACATATGAATTTCCAAAATTCATGTACATATATAGAAAAAAAATAAATGATTGAGGTCTAAATTTCGATTCCTCTTGTTTGGTTATTGGCTATCGATTCTATGACCTTTGCTATTTTTTTAAAATTTTTATTCTTATTTTTGAACTATGCTTTTTACTTTTGTAATATTTATATTAATCTCACGTTTTTTTAAATTATTGTTTGTGTATTTTTTTATGTTAGGGGATATTATCGATGTAGTAGCTCCAAGGGCTGCCCAGCAAGAAAGCAAGTAGAGAGAAGCCGTGTGGACCCCAGCAAGCTCGTTATTACCTACGCTTTCGACCACAACCACCAACTCCCGGTTACCAAATCTCACCACCACCACCACAATTCCTCCCCGTCCTCCGCCGTGATCGCCGCCGCATCCGCCGCAGCCGACTCTTCGTCCCCAGGGAGCACTACGACGTCGTCCTCCACTTCCTCCGGCGACAATACCAACGCCGCTCCGTCATCGCCGGCGGCGGCGGCGAAGTTCGAGGAGGCGGCTGCGGTTTTCGCGAGCCAGCCGGAACTGGAACTCGGCGGCGACTCGCTGATGATAAAACCGTGCATCGGCGATTTCGGGTGGTTGGCTGACGTGGCGTACGACAGAATCCTGGAGGGCCCGATTTGCGGGGGAGGCGACATATTCGACGACGCCGATGTAATGGTTTTGTCCACAAGAGGAGATGACGAGGAAGAGTCGTTATTTGCGGATCTTGGGGAATTACCCGAAGGCTCCGTGGTTTTCGGCCGACGGCGACCGGTCCAACCGGACGGACCGAACCGGACATGTGGCACAGTTTTAGATTGCTAGGGTTTTTATTTTTCTTTTTTTATTTTTTTTTCCTTCTAGGATTTGGTTCGTTCGTTTGCAATTGCCAATTGGTGATTGCTGTGGAAAACTTTACAAACAGAGAAGAGAGAGAGAGAGAGAGAGAGAGAGAGGATTTTTCTTTATAATTTATAATAATAATTTTTTTTTTTAATTTAGTTTTAAAATGGGTTTCTGTTGTACAGTGAAAGATAGTGAAGAATATTGAGAAATAATGGAATGCTGTTTCCCTTTTGAGTTTGAGCA

mRNA sequence

AAACAAACATAGTCTGCCAATCTGTCACCATGACTACTCTTTGTATTAAACCTCATCTAATTCTCTCACTCTTTCCCTTATTCAATCTCTCCGACCAAATCCCAATTCCCTTTTTTCTCTCTAATCTCAGCCCTACGATTGATCCTCATCTAACAATTTCCTACCGCCGGATTTTCTTAATCTCATCGATTCTCTGACGCTGTTCCGCCCATGGACCGCCGTGTTCGTACCAACCCATTTGTCTCCGAGCAAGAAGATCCTGAACCCACGTCCGACGACGGCTTACCGGAATCACCCTCCGACGGCAATGACTCAAAACCCGCCGCCGGTCCACCTCCCAAAAAGAGTAGAAGGGGAGTGCAGAAGCGAGTGGTGTCCGTACCGATCAGTGACGTGGAAGGATCTAAGAGCAAAGGGGAAGCATATCCACCGTCCGATTCATGGGCATGGAGAAAATACGGCCAAAAGCCAATCAAAGGCTCTCCTTATCCCAGGGGATATTATCGATGTAGTAGCTCCAAGGGCTGCCCAGCAAGAAAGCAAGTAGAGAGAAGCCGTGTGGACCCCAGCAAGCTCGTTATTACCTACGCTTTCGACCACAACCACCAACTCCCGGTTACCAAATCTCACCACCACCACCACAATTCCTCCCCGTCCTCCGCCGTGATCGCCGCCGCATCCGCCGCAGCCGACTCTTCGTCCCCAGGGAGCACTACGACGTCGTCCTCCACTTCCTCCGGCGACAATACCAACGCCGCTCCGTCATCGCCGGCGGCGGCGGCGAAGTTCGAGGAGGCGGCTGCGGTTTTCGCGAGCCAGCCGGAACTGGAACTCGGCGGCGACTCGCTGATGATAAAACCGTGCATCGGCGATTTCGGGTGGTTGGCTGACGTGGCGTACGACAGAATCCTGGAGGGCCCGATTTGCGGGGGAGGCGACATATTCGACGACGCCGATGTAATGGTTTTGTCCACAAGAGGAGATGACGAGGAAGAGTCGTTATTTGCGGATCTTGGGGAATTACCCGAAGGCTCCGTGGTTTTCGGCCGACGGCGACCGGTCCAACCGGACGGACCGAACCGGACATGTGGCACAGTTTTAGATTGCTAGGGTTTTTATTTTTCTTTTTTTATTTTTTTTTCCTTCTAGGATTTGGTTCGTTCGTTTGCAATTGCCAATTGGTGATTGCTGTGGAAAACTTTACAAACAGAGAAGAGAGAGAGAGAGAGAGAGAGAGAGAGGATTTTTCTTTATAATTTATAATAATAATTTTTTTTTTTAATTTAGTTTTAAAATGGGTTTCTGTTGTACAGTGAAAGATAGTGAAGAATATTGAGAAATAATGGAATGCTGTTTCCCTTTTGAGTTTGAGCA

Coding sequence (CDS)

ATGGACCGCCGTGTTCGTACCAACCCATTTGTCTCCGAGCAAGAAGATCCTGAACCCACGTCCGACGACGGCTTACCGGAATCACCCTCCGACGGCAATGACTCAAAACCCGCCGCCGGTCCACCTCCCAAAAAGAGTAGAAGGGGAGTGCAGAAGCGAGTGGTGTCCGTACCGATCAGTGACGTGGAAGGATCTAAGAGCAAAGGGGAAGCATATCCACCGTCCGATTCATGGGCATGGAGAAAATACGGCCAAAAGCCAATCAAAGGCTCTCCTTATCCCAGGGGATATTATCGATGTAGTAGCTCCAAGGGCTGCCCAGCAAGAAAGCAAGTAGAGAGAAGCCGTGTGGACCCCAGCAAGCTCGTTATTACCTACGCTTTCGACCACAACCACCAACTCCCGGTTACCAAATCTCACCACCACCACCACAATTCCTCCCCGTCCTCCGCCGTGATCGCCGCCGCATCCGCCGCAGCCGACTCTTCGTCCCCAGGGAGCACTACGACGTCGTCCTCCACTTCCTCCGGCGACAATACCAACGCCGCTCCGTCATCGCCGGCGGCGGCGGCGAAGTTCGAGGAGGCGGCTGCGGTTTTCGCGAGCCAGCCGGAACTGGAACTCGGCGGCGACTCGCTGATGATAAAACCGTGCATCGGCGATTTCGGGTGGTTGGCTGACGTGGCGTACGACAGAATCCTGGAGGGCCCGATTTGCGGGGGAGGCGACATATTCGACGACGCCGATGTAATGGTTTTGTCCACAAGAGGAGATGACGAGGAAGAGTCGTTATTTGCGGATCTTGGGGAATTACCCGAAGGCTCCGTGGTTTTCGGCCGACGGCGACCGGTCCAACCGGACGGACCGAACCGGACATGTGGCACAGTTTTAGATTGCTAG

Protein sequence

MDRRVRTNPFVSEQEDPEPTSDDGLPESPSDGNDSKPAAGPPPKKSRRGVQKRVVSVPISDVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLVITYAFDHNHQLPVTKSHHHHHNSSPSSAVIAAASAAADSSSPGSTTTSSSTSSGDNTNAAPSSPAAAAKFEEAAAVFASQPELELGGDSLMIKPCIGDFGWLADVAYDRILEGPICGGGDIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRRPVQPDGPNRTCGTVLDC
Homology
BLAST of Bhi05G001675 vs. TAIR 10
Match: AT3G58710.1 (WRKY DNA-binding protein 69 )

HSP 1 Score: 207.2 bits (526), Expect = 1.8e-53
Identity = 142/291 (48.80%), Postives = 167/291 (57.39%), Query Frame = 0

Query: 11  VSEQEDPE-PTSDDGLPESPSDGNDSKPAAGPPPKKSRRGVQKRVVSVPISDVEGSKSKG 70
           + E +D E  T +D +PESPS   DSK  + P PKKSRR V+KRVVSVPI+DVEGSKS+G
Sbjct: 7   IQESDDEEDETYNDVVPESPSSCEDSK-ISKPTPKKSRRNVEKRVVSVPIADVEGSKSRG 66

Query: 71  EAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLVITYAFD 130
           E YPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKL+ITYA D
Sbjct: 67  EVYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLMITYACD 126

Query: 131 HNHQLPV----TKSHHHHHNSSPSSAVIAAASAAADSSSPGSTTTSSSTSSGDNTNAAPS 190
           HNH  P     TKSHH       SS V+  A    +        T           AA  
Sbjct: 127 HNHPFPSSSANTKSHHR------SSVVLKTAKKEEEYEEEEEELT---------VTAAEE 186

Query: 191 SPAAAAKFEEAAAVFASQPELELGGDSLMIKPC---IGDFGWLADVAYDRILEGPICGGG 250
            PA                +L      L++  C   IG+FGW  D +           G 
Sbjct: 187 PPAGL--------------DLSHVDSPLLLGGCYSEIGEFGWFYDASISS------SSGS 246

Query: 251 DIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRRPVQPDGPNRTC 294
             F D  +    + G +E+ESLF DLG+LP+ + VF RR  V  +  +R C
Sbjct: 247 SNFLDVTLERGFSVGQEEDESLFGDLGDLPDCASVF-RRGTVATEEQHRRC 260

BLAST of Bhi05G001675 vs. TAIR 10
Match: AT3G58710.2 (WRKY DNA-binding protein 69 )

HSP 1 Score: 201.1 bits (510), Expect = 1.3e-51
Identity = 141/291 (48.45%), Postives = 166/291 (57.04%), Query Frame = 0

Query: 11  VSEQEDPE-PTSDDGLPESPSDGNDSKPAAGPPPKKSRRGVQKRVVSVPISDVEGSKSKG 70
           + E +D E  T +D +PESPS   DSK  + P PKK RR V+KRVVSVPI+DVEGSKS+G
Sbjct: 7   IQESDDEEDETYNDVVPESPSSCEDSK-ISKPTPKK-RRNVEKRVVSVPIADVEGSKSRG 66

Query: 71  EAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLVITYAFD 130
           E YPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKL+ITYA D
Sbjct: 67  EVYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLMITYACD 126

Query: 131 HNHQLPV----TKSHHHHHNSSPSSAVIAAASAAADSSSPGSTTTSSSTSSGDNTNAAPS 190
           HNH  P     TKSHH       SS V+  A    +        T           AA  
Sbjct: 127 HNHPFPSSSANTKSHHR------SSVVLKTAKKEEEYEEEEEELT---------VTAAEE 186

Query: 191 SPAAAAKFEEAAAVFASQPELELGGDSLMIKPC---IGDFGWLADVAYDRILEGPICGGG 250
            PA                +L      L++  C   IG+FGW  D +           G 
Sbjct: 187 PPAGL--------------DLSHVDSPLLLGGCYSEIGEFGWFYDASISS------SSGS 246

Query: 251 DIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRRPVQPDGPNRTC 294
             F D  +    + G +E+ESLF DLG+LP+ + VF RR  V  +  +R C
Sbjct: 247 SNFLDVTLERGFSVGQEEDESLFGDLGDLPDCASVF-RRGTVATEEQHRRC 259

BLAST of Bhi05G001675 vs. TAIR 10
Match: AT1G29280.1 (WRKY DNA-binding protein 65 )

HSP 1 Score: 170.6 bits (431), Expect = 1.9e-42
Identity = 121/280 (43.21%), Postives = 149/280 (53.21%), Query Frame = 0

Query: 16  DPEPTSDDGLPESPSDG--NDSKP-AAGPPPKKSRRGVQKRVVSVPISDVEGSKSKGEAY 75
           D E + + G PESP+    N  K   +   PK+SRR V+KRVV+VP+ ++EGS+ KG+  
Sbjct: 13  DHESSQETG-PESPNSSTFNGMKALISSHSPKRSRRSVEKRVVNVPMKEMEGSRHKGDTT 72

Query: 76  PPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLVITYAFDHNH 135
           PPSDSWAWRKYGQKPIKGSPYPRGYYRCSS+KGCPARKQVERSR DP+ ++ITY  +HNH
Sbjct: 73  PPSDSWAWRKYGQKPIKGSPYPRGYYRCSSTKGCPARKQVERSRDDPTMILITYTSEHNH 132

Query: 136 QLPVTKSHHHHHNSSPSSAVIAAASAAADSSSPGSTTTSSSTSSGDNTNAAPS---SPAA 195
             P+T                                  SST +G      P     P  
Sbjct: 133 PWPLT----------------------------------SSTRNGPKPKPEPKPEPEPEV 192

Query: 196 AAKFEEAAAVFASQPELELGGDSLMIKPCIGDFGWLADV--AYDRILEGPICGGGD---I 255
             + EE    F     + LG        C+ +F W  ++      ILE PI        +
Sbjct: 193 EPEAEEEDNKF-----MVLGRGIETTPSCVDEFAWFTEMETTSSTILESPIFSSEKKTAV 251

Query: 256 FDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRRPV 285
               DV V    G +E+ESLFADLGELPE SVVF  R  V
Sbjct: 253 SGADDVAVFFPMG-EEDESLFADLGELPECSVVFRHRSSV 251

BLAST of Bhi05G001675 vs. TAIR 10
Match: AT2G34830.1 (WRKY DNA-binding protein 35 )

HSP 1 Score: 121.3 bits (303), Expect = 1.3e-27
Identity = 77/156 (49.36%), Postives = 98/156 (62.82%), Query Frame = 0

Query: 45  KSRRGVQKRVVSVPISDVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSK 104
           K R+   K+VV +P      S+S GE   PSD WAWRKYGQKPIKGSPYPRGYYRCSSSK
Sbjct: 186 KRRKSQAKKVVCIPAPAAMNSRSSGEVV-PSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 245

Query: 105 GCPARKQVERSRVDPSKLVITYAFDHNHQLPVTKSHHHHHNSSPSSAVI---AAASAAAD 164
           GC ARKQVERSR DP+ LVITY  +HNH  P  ++       S SS+ +   + +S AA 
Sbjct: 246 GCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSSSSLNPSSKSSTAAA 305

Query: 165 SSSPGSTTTSSSTSSGDNTNAAPSSPAAAAKFEEAA 198
           ++SP S    +++S  +  N+   S +    F+ AA
Sbjct: 306 TTSPSSRVFQNNSSKDEPNNSNLPSSSTHPPFDAAA 340

BLAST of Bhi05G001675 vs. TAIR 10
Match: AT1G30650.1 (WRKY DNA-binding protein 14 )

HSP 1 Score: 117.9 bits (294), Expect = 1.4e-26
Identity = 76/164 (46.34%), Postives = 96/164 (58.54%), Query Frame = 0

Query: 45  KSRRGVQKRVVSVPISDVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSK 104
           K R+   K+VV +P      S+S GE   PSD WAWRKYGQKPIKGSP+PRGYYRCSSSK
Sbjct: 188 KRRKSQAKKVVCIPAPAAMNSRSSGEVV-PSDLWAWRKYGQKPIKGSPFPRGYYRCSSSK 247

Query: 105 GCPARKQVERSRVDPSKLVITYAFDHNHQLPVTKSHHHHHNSSPSSAVIAAASAAADSSS 164
           GC ARKQVERSR DP+ LVITY  +HNH  P+ ++       S +S     +S+  + S 
Sbjct: 248 GCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRNALAGSTRSSTS-----SSSNPNPSK 307

Query: 165 PGSTTTSSSTSSGDNTNAAPSSPAAAAKFEEAAAVFASQPELEL 209
           P +   +SS+    NT   PSS         +A       ++EL
Sbjct: 308 PSTANVNSSSIGSQNTIYLPSSTTPPPTLSSSAIKDERGDDMEL 345

BLAST of Bhi05G001675 vs. ExPASy Swiss-Prot
Match: Q93WV5 (Probable WRKY transcription factor 69 OS=Arabidopsis thaliana OX=3702 GN=WRKY69 PE=2 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.8e-50
Identity = 141/291 (48.45%), Postives = 166/291 (57.04%), Query Frame = 0

Query: 11  VSEQEDPE-PTSDDGLPESPSDGNDSKPAAGPPPKKSRRGVQKRVVSVPISDVEGSKSKG 70
           + E +D E  T +D +PESPS   DSK  + P PKK RR V+KRVVSVPI+DVEGSKS+G
Sbjct: 7   IQESDDEEDETYNDVVPESPSSCEDSK-ISKPTPKK-RRNVEKRVVSVPIADVEGSKSRG 66

Query: 71  EAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLVITYAFD 130
           E YPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKL+ITYA D
Sbjct: 67  EVYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLMITYACD 126

Query: 131 HNHQLPV----TKSHHHHHNSSPSSAVIAAASAAADSSSPGSTTTSSSTSSGDNTNAAPS 190
           HNH  P     TKSHH       SS V+  A    +        T           AA  
Sbjct: 127 HNHPFPSSSANTKSHHR------SSVVLKTAKKEEEYEEEEEELT---------VTAAEE 186

Query: 191 SPAAAAKFEEAAAVFASQPELELGGDSLMIKPC---IGDFGWLADVAYDRILEGPICGGG 250
            PA                +L      L++  C   IG+FGW  D +           G 
Sbjct: 187 PPAGL--------------DLSHVDSPLLLGGCYSEIGEFGWFYDASISS------SSGS 246

Query: 251 DIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRRPVQPDGPNRTC 294
             F D  +    + G +E+ESLF DLG+LP+ + VF RR  V  +  +R C
Sbjct: 247 SNFLDVTLERGFSVGQEEDESLFGDLGDLPDCASVF-RRGTVATEEQHRRC 259

BLAST of Bhi05G001675 vs. ExPASy Swiss-Prot
Match: Q9LP56 (Probable WRKY transcription factor 65 OS=Arabidopsis thaliana OX=3702 GN=WRKY65 PE=2 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 2.6e-41
Identity = 121/280 (43.21%), Postives = 149/280 (53.21%), Query Frame = 0

Query: 16  DPEPTSDDGLPESPSDG--NDSKP-AAGPPPKKSRRGVQKRVVSVPISDVEGSKSKGEAY 75
           D E + + G PESP+    N  K   +   PK+SRR V+KRVV+VP+ ++EGS+ KG+  
Sbjct: 13  DHESSQETG-PESPNSSTFNGMKALISSHSPKRSRRSVEKRVVNVPMKEMEGSRHKGDTT 72

Query: 76  PPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLVITYAFDHNH 135
           PPSDSWAWRKYGQKPIKGSPYPRGYYRCSS+KGCPARKQVERSR DP+ ++ITY  +HNH
Sbjct: 73  PPSDSWAWRKYGQKPIKGSPYPRGYYRCSSTKGCPARKQVERSRDDPTMILITYTSEHNH 132

Query: 136 QLPVTKSHHHHHNSSPSSAVIAAASAAADSSSPGSTTTSSSTSSGDNTNAAPS---SPAA 195
             P+T                                  SST +G      P     P  
Sbjct: 133 PWPLT----------------------------------SSTRNGPKPKPEPKPEPEPEV 192

Query: 196 AAKFEEAAAVFASQPELELGGDSLMIKPCIGDFGWLADV--AYDRILEGPICGGGD---I 255
             + EE    F     + LG        C+ +F W  ++      ILE PI        +
Sbjct: 193 EPEAEEEDNKF-----MVLGRGIETTPSCVDEFAWFTEMETTSSTILESPIFSSEKKTAV 251

Query: 256 FDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRRPV 285
               DV V    G +E+ESLFADLGELPE SVVF  R  V
Sbjct: 253 SGADDVAVFFPMG-EEDESLFADLGELPECSVVFRHRSSV 251

BLAST of Bhi05G001675 vs. ExPASy Swiss-Prot
Match: O64747 (Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.8e-26
Identity = 77/156 (49.36%), Postives = 98/156 (62.82%), Query Frame = 0

Query: 45  KSRRGVQKRVVSVPISDVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSK 104
           K R+   K+VV +P      S+S GE   PSD WAWRKYGQKPIKGSPYPRGYYRCSSSK
Sbjct: 186 KRRKSQAKKVVCIPAPAAMNSRSSGEVV-PSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 245

Query: 105 GCPARKQVERSRVDPSKLVITYAFDHNHQLPVTKSHHHHHNSSPSSAVI---AAASAAAD 164
           GC ARKQVERSR DP+ LVITY  +HNH  P  ++       S SS+ +   + +S AA 
Sbjct: 246 GCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSSSSLNPSSKSSTAAA 305

Query: 165 SSSPGSTTTSSSTSSGDNTNAAPSSPAAAAKFEEAA 198
           ++SP S    +++S  +  N+   S +    F+ AA
Sbjct: 306 TTSPSSRVFQNNSSKDEPNNSNLPSSSTHPPFDAAA 340

BLAST of Bhi05G001675 vs. ExPASy Swiss-Prot
Match: Q9SA80 (Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 PE=2 SV=2)

HSP 1 Score: 117.9 bits (294), Expect = 2.0e-25
Identity = 76/164 (46.34%), Postives = 96/164 (58.54%), Query Frame = 0

Query: 45  KSRRGVQKRVVSVPISDVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSK 104
           K R+   K+VV +P      S+S GE   PSD WAWRKYGQKPIKGSP+PRGYYRCSSSK
Sbjct: 188 KRRKSQAKKVVCIPAPAAMNSRSSGEVV-PSDLWAWRKYGQKPIKGSPFPRGYYRCSSSK 247

Query: 105 GCPARKQVERSRVDPSKLVITYAFDHNHQLPVTKSHHHHHNSSPSSAVIAAASAAADSSS 164
           GC ARKQVERSR DP+ LVITY  +HNH  P+ ++       S +S     +S+  + S 
Sbjct: 248 GCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRNALAGSTRSSTS-----SSSNPNPSK 307

Query: 165 PGSTTTSSSTSSGDNTNAAPSSPAAAAKFEEAAAVFASQPELEL 209
           P +   +SS+    NT   PSS         +A       ++EL
Sbjct: 308 PSTANVNSSSIGSQNTIYLPSSTTPPPTLSSSAIKDERGDDMEL 345

BLAST of Bhi05G001675 vs. ExPASy Swiss-Prot
Match: Q9FL92 (Disease resistance protein RRS1B OS=Arabidopsis thaliana OX=3702 GN=RRS1B PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 5.7e-20
Identity = 72/151 (47.68%), Postives = 89/151 (58.94%), Query Frame = 0

Query: 45   KSRR--GVQKRVVSVPISDVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSS 104
            KSRR    +KRVV V      GS+S       SD W WRKYGQKPIK SPYPR YYRC+S
Sbjct: 1158 KSRRKNNKEKRVVCVV---DRGSRS-------SDLWVWRKYGQKPIKSSPYPRSYYRCAS 1217

Query: 105  SKGCPARKQVERSRVDPSKLVITYAFDHNHQLPVTKSHHHHHNSSPSSAVIAAASAAADS 164
            SKGC ARKQVERSR DP+  VITY  +HNH  P  ++             +A ++ ++ S
Sbjct: 1218 SKGCFARKQVERSRTDPNVSVITYISEHNHPFPTLRN------------TLAGSTRSSSS 1277

Query: 165  SSPGSTTTSSSTSS----GDNTNAAPSSPAA 190
                 TT++SST S    G + +  PSSPA+
Sbjct: 1278 KCSDVTTSASSTVSQDKEGPDKSHLPSSPAS 1286

BLAST of Bhi05G001675 vs. ExPASy TrEMBL
Match: A0A1S3C0Q3 (probable WRKY transcription factor 69 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495493 PE=4 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 7.0e-154
Identity = 282/299 (94.31%), Postives = 288/299 (96.32%), Query Frame = 0

Query: 1   MDRRVRTNPFVSEQEDPEPTSDDGLPESPSDGNDSKPAAGPPPKKSRRGVQKRVVSVPIS 60
           MDRRVRTNPF+SEQEDPEPTSDDGLPESPSD NDSKP A PPPKKSRRGVQKRVVSVPI+
Sbjct: 1   MDRRVRTNPFLSEQEDPEPTSDDGLPESPSDANDSKPTAAPPPKKSRRGVQKRVVSVPIA 60

Query: 61  DVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPS 120
           DVEGS+SKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP+
Sbjct: 61  DVEGSRSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPT 120

Query: 121 KLVITYAFDHNHQLPVTKSHHHHHNSSPSSAVIAAASAAADSSSPGSTTTSSSTSSGDNT 180
           KLVITYAFDHNHQLPVTKSHHHHHNSSPSSAV+AAASA  DS SPGSTTTSSSTSSGDNT
Sbjct: 121 KLVITYAFDHNHQLPVTKSHHHHHNSSPSSAVVAAASAGTDSPSPGSTTTSSSTSSGDNT 180

Query: 181 NAAPSSPAAAAKFEEAAAVFASQPELELGGDSLMIKPCIGDFGWLADVAYDRILEGPICG 240
           NAAPSSP  AAKFEE AAVFASQPELELGGDSLMIKPCIGDFGWL +VAYDRILEGPICG
Sbjct: 181 NAAPSSP--AAKFEEVAAVFASQPELELGGDSLMIKPCIGDFGWLGEVAYDRILEGPICG 240

Query: 241 GGDIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRRPVQPDGPNRTCGTVLDC 300
           GGDIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRR VQPDGPNRTCGTVLDC
Sbjct: 241 GGDIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRRTVQPDGPNRTCGTVLDC 297

BLAST of Bhi05G001675 vs. ExPASy TrEMBL
Match: A0A5A7SK87 (Putative WRKY transcription factor 69 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003230 PE=4 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 9.1e-154
Identity = 282/299 (94.31%), Postives = 287/299 (95.99%), Query Frame = 0

Query: 1   MDRRVRTNPFVSEQEDPEPTSDDGLPESPSDGNDSKPAAGPPPKKSRRGVQKRVVSVPIS 60
           MDRRVRTNPF+SEQEDPEPTSDDGLPESPSD NDSKP A PPPKKSRRGVQKRVVSVPI+
Sbjct: 1   MDRRVRTNPFLSEQEDPEPTSDDGLPESPSDANDSKPTAAPPPKKSRRGVQKRVVSVPIA 60

Query: 61  DVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPS 120
           DVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP+
Sbjct: 61  DVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPT 120

Query: 121 KLVITYAFDHNHQLPVTKSHHHHHNSSPSSAVIAAASAAADSSSPGSTTTSSSTSSGDNT 180
           KLVITYAFDHNHQLPVTKSHHHHHNSSPSSAV+AAASA  DS SPGSTTTSSSTSSGDNT
Sbjct: 121 KLVITYAFDHNHQLPVTKSHHHHHNSSPSSAVVAAASAGTDSPSPGSTTTSSSTSSGDNT 180

Query: 181 NAAPSSPAAAAKFEEAAAVFASQPELELGGDSLMIKPCIGDFGWLADVAYDRILEGPICG 240
           NAAPSSP  AAKFEE AAVFASQPELELGGDSLMIKPCIGDFGWL +VAYDRILEGPICG
Sbjct: 181 NAAPSSP--AAKFEEVAAVFASQPELELGGDSLMIKPCIGDFGWLGEVAYDRILEGPICG 240

Query: 241 GGDIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRRPVQPDGPNRTCGTVLDC 300
           GGDIFDD DVMVLSTRGDDEEESLFADLGELPEGSVVFGRRR VQPDGPNRTCGTVLDC
Sbjct: 241 GGDIFDDTDVMVLSTRGDDEEESLFADLGELPEGSVVFGRRRTVQPDGPNRTCGTVLDC 297

BLAST of Bhi05G001675 vs. ExPASy TrEMBL
Match: A0A1S3C0K9 (probable WRKY transcription factor 69 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103495493 PE=4 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 5.0e-152
Identity = 281/299 (93.98%), Postives = 287/299 (95.99%), Query Frame = 0

Query: 1   MDRRVRTNPFVSEQEDPEPTSDDGLPESPSDGNDSKPAAGPPPKKSRRGVQKRVVSVPIS 60
           MDRRVRTNPF+SEQEDPEPTSDDGLPESPSD NDSKP A PPPKK RRGVQKRVVSVPI+
Sbjct: 1   MDRRVRTNPFLSEQEDPEPTSDDGLPESPSDANDSKPTAAPPPKK-RRGVQKRVVSVPIA 60

Query: 61  DVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPS 120
           DVEGS+SKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP+
Sbjct: 61  DVEGSRSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPT 120

Query: 121 KLVITYAFDHNHQLPVTKSHHHHHNSSPSSAVIAAASAAADSSSPGSTTTSSSTSSGDNT 180
           KLVITYAFDHNHQLPVTKSHHHHHNSSPSSAV+AAASA  DS SPGSTTTSSSTSSGDNT
Sbjct: 121 KLVITYAFDHNHQLPVTKSHHHHHNSSPSSAVVAAASAGTDSPSPGSTTTSSSTSSGDNT 180

Query: 181 NAAPSSPAAAAKFEEAAAVFASQPELELGGDSLMIKPCIGDFGWLADVAYDRILEGPICG 240
           NAAPSSP  AAKFEE AAVFASQPELELGGDSLMIKPCIGDFGWL +VAYDRILEGPICG
Sbjct: 181 NAAPSSP--AAKFEEVAAVFASQPELELGGDSLMIKPCIGDFGWLGEVAYDRILEGPICG 240

Query: 241 GGDIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRRPVQPDGPNRTCGTVLDC 300
           GGDIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRR VQPDGPNRTCGTVLDC
Sbjct: 241 GGDIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRRTVQPDGPNRTCGTVLDC 296

BLAST of Bhi05G001675 vs. ExPASy TrEMBL
Match: A0A1S6YJF1 (WRKY transcription factor 1-like protein OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 5.5e-151
Identity = 281/300 (93.67%), Postives = 285/300 (95.00%), Query Frame = 0

Query: 1   MDRRVRTNPFVSEQEDPEPTSDDGLPESPSDGNDSKPAAGPPPKKSRRGVQKRVVSVPIS 60
           MDRRVRTNPF+SEQEDPE TSDDGLPESPSDGNDSKPAAGPPPKKSRRGVQKRVVSVPI 
Sbjct: 1   MDRRVRTNPFLSEQEDPEATSDDGLPESPSDGNDSKPAAGPPPKKSRRGVQKRVVSVPIG 60

Query: 61  DVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPS 120
           DVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP+
Sbjct: 61  DVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPT 120

Query: 121 KLVITYAFDHNHQLPVTKS-HHHHHNSSPSSAVIAAASAAADSSSPGSTTTSSSTSSGDN 180
           KLVITYAFDHNHQLP TKS HHHHHNSSPSSA IAAA A  D SSPGSTTTSSSTSSGDN
Sbjct: 121 KLVITYAFDHNHQLPATKSHHHHHHNSSPSSASIAAAVAVPDDSSPGSTTTSSSTSSGDN 180

Query: 181 TNAAPSSPAAAAKFEEAAAVFASQPELELGGDSLMIKPCIGDFGWLADVAYDRILEGPIC 240
           TNA P+SP  AAKFEEAAAVFASQPELELGGDSLMIKPCIGDFGW ADVAYDRILEGPIC
Sbjct: 181 TNATPTSP-TAAKFEEAAAVFASQPELELGGDSLMIKPCIGDFGWFADVAYDRILEGPIC 240

Query: 241 GGGDIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRRPVQPDGPNRTCGTVLDC 300
           GGGDIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRR V+PDGPNRTCG VLDC
Sbjct: 241 GGGDIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRRAVEPDGPNRTCGRVLDC 299

BLAST of Bhi05G001675 vs. ExPASy TrEMBL
Match: E7CEY2 (WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY47 PE=2 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 2.7e-150
Identity = 281/300 (93.67%), Postives = 287/300 (95.67%), Query Frame = 0

Query: 1   MDRRVRTNPFVSEQEDPEPTSDDGLPESPSDGNDSKPAAGPPPKKSRRGVQKRVVSVPIS 60
           MDRRVRTNPF+SEQEDPE TSDDGLPESPSD NDSKP A PPPKKSRRGVQKRVVSVPI+
Sbjct: 1   MDRRVRTNPFLSEQEDPEATSDDGLPESPSDCNDSKPTAAPPPKKSRRGVQKRVVSVPIT 60

Query: 61  DVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPS 120
           DVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDP+
Sbjct: 61  DVEGSKSKGEAYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPT 120

Query: 121 KLVITYAFDHNHQLPVTKS-HHHHHNSSPSSAVIAAASAAADSSSPGSTTTSSSTSSGDN 180
           KLVITYAFDHNHQLPVTKS HHHHHNSSPSSAVIAA SAA D  SPGSTTTSSSTSSGDN
Sbjct: 121 KLVITYAFDHNHQLPVTKSHHHHHHNSSPSSAVIAAVSAATDFPSPGSTTTSSSTSSGDN 180

Query: 181 TNAAPSSPAAAAKFEEAAAVFASQPELELGGDSLMIKPCIGDFGWLADVAYDRILEGPIC 240
           TNAAPSSP  AAKFEEAAAVFASQPELELGGDSLMIKPCIGDFGWL +VAYDRILEGPIC
Sbjct: 181 TNAAPSSP--AAKFEEAAAVFASQPELELGGDSLMIKPCIGDFGWLGEVAYDRILEGPIC 240

Query: 241 GGGDIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRRPVQPDGPNRTCGTVLDC 300
           GGGDIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRR VQP+GPNRTCGTVL+C
Sbjct: 241 GGGDIFDDADVMVLSTRGDDEEESLFADLGELPEGSVVFGRRRTVQPNGPNRTCGTVLNC 298

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G58710.11.8e-5348.80WRKY DNA-binding protein 69 [more]
AT3G58710.21.3e-5148.45WRKY DNA-binding protein 69 [more]
AT1G29280.11.9e-4243.21WRKY DNA-binding protein 65 [more]
AT2G34830.11.3e-2749.36WRKY DNA-binding protein 35 [more]
AT1G30650.11.4e-2646.34WRKY DNA-binding protein 14 [more]
Match NameE-valueIdentityDescription
Q93WV51.8e-5048.45Probable WRKY transcription factor 69 OS=Arabidopsis thaliana OX=3702 GN=WRKY69 ... [more]
Q9LP562.6e-4143.21Probable WRKY transcription factor 65 OS=Arabidopsis thaliana OX=3702 GN=WRKY65 ... [more]
O647471.8e-2649.36Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 ... [more]
Q9SA802.0e-2546.34Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 ... [more]
Q9FL925.7e-2047.68Disease resistance protein RRS1B OS=Arabidopsis thaliana OX=3702 GN=RRS1B PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A1S3C0Q37.0e-15494.31probable WRKY transcription factor 69 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A5A7SK879.1e-15494.31Putative WRKY transcription factor 69 isoform X1 OS=Cucumis melo var. makuwa OX=... [more]
A0A1S3C0K95.0e-15293.98probable WRKY transcription factor 69 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A1S6YJF15.5e-15193.67WRKY transcription factor 1-like protein OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
E7CEY22.7e-15093.67WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY47 PE=2 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 74..134
e-value: 7.5E-35
score: 131.8
IPR003657WRKY domainPFAMPF03106WRKYcoord: 76..133
e-value: 7.9E-25
score: 86.9
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 75..135
score: 31.19219
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 60..136
e-value: 2.2E-29
score: 103.0
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 70..135
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 146..186
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..114
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 136..188
NoneNo IPR availablePANTHERPTHR32096:SF19OS01G0750100 PROTEINcoord: 21..283
IPR044810WRKY transcription factor, plantPANTHERPTHR32096WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATEDcoord: 21..283

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi05M001675Bhi05M001675mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0000976 transcription cis-regulatory region binding
molecular_function GO:0043565 sequence-specific DNA binding