Bhi05G001433 (gene) Wax gourd (B227) v1

Overview


Name	Bhi05G001433
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	O-methyltransferase family protein
Location	chr5: 54418248 .. 54419322 (+)
RNA-Seq Expression	Bhi05G001433
Synteny	Bhi05G001433

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACTTTGCTTCCAAAGAATCCATACCAAACAAGGAAGAAGAAGAAGAAGAGGTTCGTGTTCAAATTTGGAAATACATATTCGGTTTCGTGGAAATGGCAATTGTAAAATGTGCCATAGAACTCAAAATTGTTGATACAATTGAAAGTCATGGAAGCTCCATGACACTCTCCCAATTATCTTCAGCTTTGAACTATTCTTTCTCACTCCTATACCGAATCTGGAGATTCTTAGTCCCTCGTGGAATTTTCAAACAAGAAATCACTCAAGAAAATCTCACTAGCTATGCTCAAACACCTTTGTCTAGGCTGCTTGCAAGTAGCAGCAAGAACAGCATGGCTCCATTTCTTCTATTGGAAAGCAACCCAGTGATGTTGGCACCATGGCATGGCCTCAGTGCTCGCATTAAAGGCACCGGAGAAACCCCATTTGAGGCTGCTCATGGGAAAGATGTATGGAGCTTTGCAGCAGCTGATCCAATACACAACATAGTAATAAATGATGCTATGTCATGTACTGCAAGAGTTCTTACTGTGCCTGCAATACTTGAAGATTGTCCAGAGATTTTTGAAGGAGATGGAAGTTTAGTTGATGTGGGAGGGGGAAATGGAACTTGCTTGAGCATGATAGTCAAAGCTTGTCCTTGGATTAAAGGCATTAACTTTGATCTTCCAGAATGTCATTTCTGTGAATCTGTTGGTTGGAATATGTTGGATTCCATTCCTAAGGCTAACGCAGCTTGTTAAATTTAATTTAGTCTGTTACAGTTGTATCTGTTTGGTCATTGAACTTTCAGAAGTATCTTATACACTAAATTGTTATAAATTTTCAAAATACATGGACTAAATTGTTTTTAAATAGAGTTCAAGAACTAAATACAAAAGAAGAAGATTTTTTTTAACCTAAAACGTGATAGAACTAGTACGTATGTGAAGTTTAGATTCACCTTCCACTGCATCTTTATATATGCTGAAATCTTTTGGATAAAAGAAGAATTATATTAACTTTTGTGATATTGTTTTATAGAGTGGGTTCTACATGACTGGGACGATGAAACATGTATCAAAATTTTGA

mRNA sequence

ATGGACTTTGCTTCCAAAGAATCCATACCAAACAAGGAAGAAGAAGAAGAAGAGGTTCGTGTTCAAATTTGGAAATACATATTCGGTTTCGTGGAAATGGCAATTGTAAAATGTGCCATAGAACTCAAAATTGTTGATACAATTGAAAGTCATGGAAGCTCCATGACACTCTCCCAATTATCTTCAGCTTTGAACTATTCTTTCTCACTCCTATACCGAATCTGGAGATTCTTAGTCCCTCGTGGAATTTTCAAACAAGAAATCACTCAAGAAAATCTCACTAGCTATGCTCAAACACCTTTGTCTAGGCTGCTTGCAAGTAGCAGCAAGAACAGCATGGCTCCATTTCTTCTATTGGAAAGCAACCCAGTGATGTTGGCACCATGGCATGGCCTCAGTGCTCGCATTAAAGGCACCGGAGAAACCCCATTTGAGGCTGCTCATGGGAAAGATGTATGGAGCTTTGCAGCAGCTGATCCAATACACAACATAGTAATAAATGATGCTATGTCATGTACTGCAAGAGTTCTTACTGTGCCTGCAATACTTGAAGATTGTCCAGAGATTTTTGAAGGAGATGGAAGTTTAGTTGATGTGGGAGGGGGAAATGGAACTTGCTTGAGCATGATAGTCAAAGCTTGTCCTTGGATTAAAGGCATTAACTTTGATCTTCCAGAATGTCATTTCTGTGAATCTAGTGGGTTCTACATGACTGGGACGATGAAACATGTATCAAAATTTTGA

Coding sequence (CDS)

ATGGACTTTGCTTCCAAAGAATCCATACCAAACAAGGAAGAAGAAGAAGAAGAGGTTCGTGTTCAAATTTGGAAATACATATTCGGTTTCGTGGAAATGGCAATTGTAAAATGTGCCATAGAACTCAAAATTGTTGATACAATTGAAAGTCATGGAAGCTCCATGACACTCTCCCAATTATCTTCAGCTTTGAACTATTCTTTCTCACTCCTATACCGAATCTGGAGATTCTTAGTCCCTCGTGGAATTTTCAAACAAGAAATCACTCAAGAAAATCTCACTAGCTATGCTCAAACACCTTTGTCTAGGCTGCTTGCAAGTAGCAGCAAGAACAGCATGGCTCCATTTCTTCTATTGGAAAGCAACCCAGTGATGTTGGCACCATGGCATGGCCTCAGTGCTCGCATTAAAGGCACCGGAGAAACCCCATTTGAGGCTGCTCATGGGAAAGATGTATGGAGCTTTGCAGCAGCTGATCCAATACACAACATAGTAATAAATGATGCTATGTCATGTACTGCAAGAGTTCTTACTGTGCCTGCAATACTTGAAGATTGTCCAGAGATTTTTGAAGGAGATGGAAGTTTAGTTGATGTGGGAGGGGGAAATGGAACTTGCTTGAGCATGATAGTCAAAGCTTGTCCTTGGATTAAAGGCATTAACTTTGATCTTCCAGAATGTCATTTCTGTGAATCTAGTGGGTTCTACATGACTGGGACGATGAAACATGTATCAAAATTTTGA

Protein sequence

MDFASKESIPNKEEEEEEVRVQIWKYIFGFVEMAIVKCAIELKIVDTIESHGSSMTLSQLSSALNYSFSLLYRIWRFLVPRGIFKQEITQENLTSYAQTPLSRLLASSSKNSMAPFLLLESNPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPAILEDCPEIFEGDGSLVDVGGGNGTCLSMIVKACPWIKGINFDLPECHFCESSGFYMTGTMKHVSKF

Homology

[Close]

BLAST of Bhi05G001433 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 201.8 bits (512), Expect = 6.3e-52
Identity = 111/221 (50.23%), Postives = 151/221 (68.33%), Query Frame = 0


Query: 14  EEEEEVRVQIWKYIFGFVEMAIVKCAIELKIVDTIESHGSS--MTLSQLSSALNYSFSLL 73
           +EE +  + IWKY+FGF ++A  KCAI+LKI + IE+H SS  +TL++LSSA++ S S L
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPSHL 83

Query: 74  YRIWRFLVPRGIFKQEITQENL-TSYAQTPLSRLLASSSKN--SMAPFLLLESNPVMLAP 133
            RI RFLV +GIFK+  T++ L T Y  TPLSR L  + ++  S+APF+L E+ P MLAP
Sbjct: 84  RRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEMLAP 143

Query: 134 WHGL----SARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPAILE 193
           W  L    S+ + G+   PF+A HGKDVWSFA  +P  + +IN+AM+C AR   VP +  
Sbjct: 144 WLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDAR-RVVPRVAG 203

Query: 194 DCPEIFEGDGSLVDVGGGNGTCLSMIVKACPWIKGINFDLP 226
            C  +F+G  ++VDVGGG G  + M+VK  PWIKG NFDLP
Sbjct: 204 ACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLP 243

[Close]

BLAST of Bhi05G001433 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 152.9 bits (385), Expect = 3.3e-37
Identity = 91/223 (40.81%), Postives = 128/223 (57.40%), Query Frame = 0


Query: 6   KESIPNKEEEEEEVRVQIWKYIFGFVEMAIVKCAIELKIVDTIESHGSS--MTLSQLSSA 65
           +ES  N  +EE +  + IW+Y+FGF ++A  KCAI+LKI + IE+H SS  +TLS+LSSA
Sbjct: 2   EESKRNLLDEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSA 61

Query: 66  LNYSFSLLYRIWRFLVPRGIFKQEITQENL-TSYAQTPLSRLLASSSKNSMAPFLLLESN 125
           ++ S S L RI RFLV +G+FK+  T++ L T Y  TPLSR +  +              
Sbjct: 62  VSASPSHLRRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMMITK------------- 121

Query: 126 PVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPAI 185
                                    HGKD+W+FA  +  H+ +IN+AM+C AR   VP +
Sbjct: 122 ------------------------LHGKDLWAFAQDNLCHSQLINEAMACDAR-RVVPRV 181

Query: 186 LEDCPEIFEGDGSLVDVGGGNGTCLSMIVKACPWIKGINFDLP 226
              C  +F+G  ++VDVGGG G  + ++VK  PWIKG NFDLP
Sbjct: 182 AGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLP 186

[Close]

BLAST of Bhi05G001433 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 84.3 bits (207), Expect = 1.5e-16
Identity = 65/199 (32.66%), Postives = 102/199 (51.26%), Query Frame = 0


Query: 36  VKCAIELKIVDTIESHGSSMTLSQLSSALNYSFS----LLYRIWRFLVPRGIF---KQEI 95
           +K A+EL +++ +  +GS M+ ++++S L         +L RI R L    +     +++
Sbjct: 36  LKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNRKL 95

Query: 96  TQENLTS-YAQTPLSRLLASSSKN-SMAPFLLLESNPVMLAPWHGLSARIKGTGETPFEA 155
           + + +   Y   P+ + L  +    S+A   L+  + V++  W+ L   I   G  PF  
Sbjct: 96  SGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAIL-DGGIPFNK 155

Query: 156 AHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPAILEDCPEIFEGDGSLVDVGGGNGTC 215
           A+G   + +   DP  N V N+ MS     +T+  ILE   + FEG  SLVDVGGG G  
Sbjct: 156 AYGMSAFEYHGTDPRFNKVFNNGMS-NHSTITMKKILETY-KGFEGLTSLVDVGGGIGAT 215

Query: 216 LSMIVKACPWIKGINFDLP 226
           L MIV   P +KGINFDLP
Sbjct: 216 LKMIVSKYPNLKGINFDLP 231

[Close]

BLAST of Bhi05G001433 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 77.0 bits (188), Expect = 2.3e-14
Identity = 69/233 (29.61%), Postives = 103/233 (44.21%), Query Frame = 0


Query: 12  KEEEEEEVRVQIWKYIFGFVEMAIVKCAIELKIVDTIESHGSSMTLSQLSSALNYSFS-- 71
           +E +E+ V +Q    +       ++K A EL ++DTI + G+   LS    A +      
Sbjct: 22  QEVDEKMVSLQAESIVNTVAFPMVLKAAFELGVIDTIAAAGNDTWLSPCEIACSLPTKPT 81

Query: 72  ------LLYRIWRFLVPRGIFKQEI--TQENLTS------YAQTPLSR--LLASSSKNSM 131
                 LL R+   LV   I K  +  T EN  +      YA  P+ +  L  S    S+
Sbjct: 82  NPEAPVLLDRMLSLLVSHSILKCRMIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGSL 141

Query: 132 APFLLLESNPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCT 191
            P  +L    V    W  L   I   G   F +AHG  ++ +  +D     + N AMS  
Sbjct: 142 VPLFMLLHTQVFFKTWTNLKDVIL-EGRDAFNSAHGMKIFEYINSDQPFAELFNRAMSEP 201

Query: 192 ARVLTVPAILEDCPEIFEGDGSLVDVGGGNGTCLSMIVKACPWIKGINFDLPE 227
           + ++    +  D    FE   +LVDVGGGNGT L ++    P IKG+NFDL +
Sbjct: 202 STMIMKKVL--DVYRGFEDVNTLVDVGGGNGTVLGLVTSKYPHIKGVNFDLAQ 251

[Close]

BLAST of Bhi05G001433 vs. TAIR 10
Match: AT5G53810.1 (O-methyltransferase family protein )

HSP 1 Score: 77.0 bits (188), Expect = 2.3e-14
Identity = 70/237 (29.54%), Postives = 111/237 (46.84%), Query Frame = 0


Query: 7   ESIPNKEEEEEEVRVQIWKYIFGFVEMAIVKCAIELKIVDTIES-HGSSMTLSQLSSALN 66
           + +  ++E EEE R+   +         ++K A+EL ++DTI +  G  + LS    AL 
Sbjct: 13  DRVKEEQEVEEEARLLARRLANAAASPMVLKAALELGVIDTITTVGGGDLWLSPSEIALR 72

Query: 67  Y--------SFSLLYRIWRFLVPRGIFK-QEITQENLTS------YAQTPLSRLLASSS- 126
                    + +LL R+ RFLV   + K + + +EN  +      YA  P+ + L + S 
Sbjct: 73  LPTKPCNLEAPALLDRMLRFLVSHSVLKCRTVIEENGQTGKVERVYAAEPVCKYLLNKSD 132

Query: 127 --KNSMAPFLLLESNPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVIN 186
               S A   +L+ + V +  W  L   I   G   F +AHG  ++ +  AD     V N
Sbjct: 133 DVSGSFASLFMLDLSDVFIKTWTHLEDVIL-EGRDAFSSAHGMKLFEYIQADERFGKVFN 192

Query: 187 DAMSCTARVLTVPAILEDCPEIFEGDGSLVDVGGGNGTCLSMIVKACPWIKGINFDL 225
            AM  ++ ++T   +     E F+   +LVDVGGG G  L +I    P + GINFDL
Sbjct: 193 RAMLESSTMVTEKVL--KFYEGFKDVKTLVDVGGGLGNTLGLITSKYPHLIGINFDL 246

[Close]

BLAST of Bhi05G001433 vs. ExPASy Swiss-Prot
Match: Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 8.8e-51
Identity = 111/221 (50.23%), Postives = 151/221 (68.33%), Query Frame = 0


Query: 14  EEEEEVRVQIWKYIFGFVEMAIVKCAIELKIVDTIESHGSS--MTLSQLSSALNYSFSLL 73
           +EE +  + IWKY+FGF ++A  KCAI+LKI + IE+H SS  +TL++LSSA++ S S L
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPSHL 83

Query: 74  YRIWRFLVPRGIFKQEITQENL-TSYAQTPLSRLLASSSKN--SMAPFLLLESNPVMLAP 133
            RI RFLV +GIFK+  T++ L T Y  TPLSR L  + ++  S+APF+L E+ P MLAP
Sbjct: 84  RRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEMLAP 143

Query: 134 WHGL----SARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPAILE 193
           W  L    S+ + G+   PF+A HGKDVWSFA  +P  + +IN+AM+C AR   VP +  
Sbjct: 144 WLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDAR-RVVPRVAG 203

Query: 194 DCPEIFEGDGSLVDVGGGNGTCLSMIVKACPWIKGINFDLP 226
            C  +F+G  ++VDVGGG G  + M+VK  PWIKG NFDLP
Sbjct: 204 ACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLP 243

[Close]

BLAST of Bhi05G001433 vs. ExPASy Swiss-Prot
Match: Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 2.3e-35
Identity = 88/220 (40.00%), Postives = 127/220 (57.73%), Query Frame = 0


Query: 14  EEEEEVRVQIWKYIFGFVEMAIVKCAIELKIVDTIESHGSSMTLSQLSSAL-------NY 73
           EE  + + +IW+++F FV+   +KCA+EL I D I SHG  +T+S++  +L       + 
Sbjct: 5   EERLKGQAEIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSSP 64

Query: 74  SFSLLYRIWRFLVPRGIFKQEITQE-NLTSYAQTPLSRLLASSSKNSMAPFLLLESNPVM 133
           +   L RI R LV + +F  E+ QE N   Y  T  S+ L   SK +++P +L E+NP++
Sbjct: 65  NIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPIL 124

Query: 134 LAPWHGLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPAILED 193
           L PW  L  +      +PFE AHG ++W  A ADP  N  +N AM C+   +    +LE 
Sbjct: 125 LKPWQYL-GKCAQEKSSPFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEMLLEY 184

Query: 194 CPEIFEGDGSLVDVGGGNGTCLSMIVKACPWIKGINFDLP 226
                   GSLVDVGGG G+ ++ IVKA P I+GINFDLP
Sbjct: 185 KDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLP 223

[Close]

BLAST of Bhi05G001433 vs. ExPASy Swiss-Prot
Match: B0ZB55 (Desmethylxanthohumol 6'-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT1 PE=1 SV=2)

HSP 1 Score: 146.0 bits (367), Expect = 5.7e-34
Identity = 86/214 (40.19%), Postives = 127/214 (59.35%), Query Frame = 0


Query: 22  QIWKYIFGFVEMAIVKCAIELKIVDTIESHGSSMTLSQLSSAL----NYSFS----LLYR 81
           QIW+ +F FV+   +KCAIEL+I D I SHG  +TLSQ++S +    N S S     L R
Sbjct: 9   QIWQLMFSFVDSMALKCAIELRIADIIHSHGKPITLSQIASGIRSNSNSSISPNIPYLSR 68

Query: 82  IWRFLVPRGIF--KQEITQENLTSYAQTPLSRLLASSSKNSMAPFLLLESNPVMLAPWHG 141
           I RFLV + IF   QE   E ++ Y  +  SR L    K+S+AP +L++++P+ +A WH 
Sbjct: 69  IMRFLVRKNIFTEHQEDNDEVISLYGLSDSSRWLLRDFKSSLAPMVLMQTHPLSMAVWHF 128

Query: 142 LSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPAILEDCPEIFE 201
           L   ++ +  T FE AHG ++W F++A+P  N + N+AM+    +     +      +  
Sbjct: 129 LEDYVRNSSNT-FEKAHGCNIWEFSSANPDFNKIFNNAMASIVPIYMGAVLSSYKDGLGC 188

Query: 202 GDGSLVDVGGGNGTCLSMIVKACPWIKGINFDLP 226
             G++VDVGGG G  +S ++K  P IKGINFDLP
Sbjct: 189 IKGTVVDVGGGTGGSISELMKYYPNIKGINFDLP 221

[Close]

BLAST of Bhi05G001433 vs. ExPASy Swiss-Prot
Match: B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.1e-32
Identity = 92/232 (39.66%), Postives = 136/232 (58.62%), Query Frame = 0


Query: 23  IWKYIFGFVEMAIVKCAIELKIVDTIESHGSSMTLSQLSSAL----NYSFSLLYRIWRFL 82
           +WK I G  +  ++KCA+EL+I D + SH + +TL+Q++S++    + + S L RI R L
Sbjct: 19  VWKSINGIADFMVMKCALELRIPDIVHSHSAPITLAQIASSVPDSPSLNLSYLSRIMRLL 78

Query: 83  VPRGIFKQEITQE-NLTSYAQTPLSRLLASSS----KNSMAPFLLLESNPVMLAPWHGLS 142
           V R IF Q  + +     Y  T  SRLL S +    + ++APF+   ++P + APW  L+
Sbjct: 79  VRRKIFSQHKSLDGEEVLYGPTHSSRLLLSKTTLPDQVTLAPFVAFMTHPYLSAPWSCLA 138

Query: 143 ARIKGTGETPFEAAH-GKDVWSFAAADPIHNIVINDAMSCTARVLTVPAILEDCPEIFEG 202
             +K  G   FE  H G+ +W  +  +P  N V ND M+ TAR+ T+ AIL +  ++F G
Sbjct: 139 RCVK-EGGNGFEMVHGGRQLWDLSPGNPEFNKVFNDGMASTARITTM-AILSEYRDVFCG 198

Query: 203 DGSLVDVGGGNGTCLSMIVKACPWIKGINFDLPECHFCESSGFYMTGTMKHV 245
             SLVDVGG  G  +S IVK+ P IKGIN+DLP  H   ++  Y TG + HV
Sbjct: 199 ICSLVDVGGEFGGSISAIVKSHPHIKGINYDLP--HVVATAPTY-TGLVSHV 245

[Close]

BLAST of Bhi05G001433 vs. ExPASy Swiss-Prot
Match: B0ZB57 (Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.8e-32
Identity = 91/228 (39.91%), Postives = 121/228 (53.07%), Query Frame = 0


Query: 9   IPNKEEEEEEVRVQ--IWKYIFGFVEMAIVKCAIELKIVDTIESHGSSMTLSQLSSAL-- 68
           I N+E   E +  Q  IW  IF F+    +KCAI+L I D I +HG  MT+SQL+ AL  
Sbjct: 17  ILNEENSTEILGAQAHIWNQIFNFINSMSLKCAIQLGIPDIINNHGKPMTISQLTLALPI 76

Query: 69  -NYSFSLLYRIWRFLVPRGIF---KQEITQE---NLTSYAQTPLSRLLASSSKNSMAPFL 128
                  +YR+ R L+  G F   K E+ +E       Y  T  S+LL   +  S+ PFL
Sbjct: 77  NRKKSPCVYRLMRILIHSGFFALQKAEVGEEGGGEEEGYVITDASKLLLKDNPMSVTPFL 136

Query: 129 LLESNPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVL 188
           L   +PVM  PW  LS   +    TPF+ A+G   W + + +P      NDAM+  AR++
Sbjct: 137 LAMLDPVMTKPWDFLSNWFQNGDPTPFDTANGMAFWDYGSHEPKLARFFNDAMASDARLV 196

Query: 189 TVPAILEDCPEIFEGDGSLVDVGGGNGTCLSMIVKACPWIKGINFDLP 226
           T   ++E C  +FEG  SLVDVGGG GT  S I  A P I+   FDLP
Sbjct: 197 T-SVVIEKCKGVFEGVESLVDVGGGTGTVASSIAAAFPHIQCTVFDLP 243

[Close]

BLAST of Bhi05G001433 vs. ExPASy TrEMBL
Match: A0A0A0K2N4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G017720 PE=3 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 1.4e-104
Identity = 199/237 (83.97%), Postives = 212/237 (89.45%), Query Frame = 0


Query: 1   MDFASKESIPNKEEEEEEVRVQIWKYIFGFVEMAIVKCAIELKIVDTIESHGSSMTLSQL 60
           MDFA ++SIP K+EEEEE RV+IWKYIFGFVEMAIVKCAIEL+I DTIESHGS MTLSQL
Sbjct: 1   MDFAPQKSIPKKQEEEEEARVEIWKYIFGFVEMAIVKCAIELRIGDTIESHGSPMTLSQL 60

Query: 61  SSALNYSFSLLYRIWRFLVPRGIFKQEITQENLTSYAQTPLSRLLASSSKNSMAPFLLLE 120
           S+ALN S SLLYRI RFLV RGIFKQEI + N+ SY QTPLSRLLASS+ NSMAPFLLLE
Sbjct: 61  STALNCSASLLYRILRFLVRRGIFKQEINEANVISYDQTPLSRLLASSNNNSMAPFLLLE 120

Query: 121 SNPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVP 180
           S+PVMLAPWH LSARIKG GETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVP
Sbjct: 121 SSPVMLAPWHRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVP 180

Query: 181 AILEDCPEIFEGDGSLVDVGGGNGTCLSMIVKACPWIKGINFDLPECHFCESSGFYM 238
           AILE+CP+IFEG GSLVDVGGGNGTCLSMIVKA PWIKGINFDLP  H   SS  Y+
Sbjct: 181 AILEECPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLP--HVISSSQQYI 235

[Close]

BLAST of Bhi05G001433 vs. ExPASy TrEMBL
Match: A0A5D3DJ08 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004240 PE=3 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 1.3e-100
Identity = 197/237 (83.12%), Postives = 207/237 (87.34%), Query Frame = 0


Query: 1   MDFASKESIPNKEEEEEEVRVQIWKYIFGFVEMAIVKCAIELKIVDTIESHGSSMTLSQL 60
           M+FA + SI  K  EEEE RVQIWKYIFGFVEMAIVKCAIEL+I DTIESHGS MTLSQL
Sbjct: 1   MNFAPQVSISKK--EEEEARVQIWKYIFGFVEMAIVKCAIELRIGDTIESHGSPMTLSQL 60

Query: 61  SSALNYSFSLLYRIWRFLVPRGIFKQEITQENLTSYAQTPLSRLLASSSKNSMAPFLLLE 120
           S+ALN S SLLYRI RFLV RGIFKQEIT+EN+ SY  TPLSRLLASS+ NSMAP LLLE
Sbjct: 61  STALNCSSSLLYRILRFLVRRGIFKQEITEENVISYDHTPLSRLLASSNNNSMAPLLLLE 120

Query: 121 SNPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVP 180
           S+PVMLAPWH LSARIKG GETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARV TVP
Sbjct: 121 SSPVMLAPWHRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVHTVP 180

Query: 181 AILEDCPEIFEGDGSLVDVGGGNGTCLSMIVKACPWIKGINFDLPECHFCESSGFYM 238
           AILEDCP+IFEG GSLVDVGGGNGTCLSMIVKA PWIKGINFDLP  H   SS  Y+
Sbjct: 181 AILEDCPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLP--HVISSSQQYI 233

[Close]

BLAST of Bhi05G001433 vs. ExPASy TrEMBL
Match: A0A1S3BII4 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490252 PE=3 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 1.3e-100
Identity = 197/237 (83.12%), Postives = 207/237 (87.34%), Query Frame = 0


Query: 1   MDFASKESIPNKEEEEEEVRVQIWKYIFGFVEMAIVKCAIELKIVDTIESHGSSMTLSQL 60
           M+FA + SI  K  EEEE RVQIWKYIFGFVEMAIVKCAIEL+I DTIESHGS MTLSQL
Sbjct: 1   MNFAPQVSISKK--EEEEARVQIWKYIFGFVEMAIVKCAIELRIGDTIESHGSPMTLSQL 60

Query: 61  SSALNYSFSLLYRIWRFLVPRGIFKQEITQENLTSYAQTPLSRLLASSSKNSMAPFLLLE 120
           S+ALN S SLLYRI RFLV RGIFKQEIT+EN+ SY  TPLSRLLASS+ NSMAP LLLE
Sbjct: 61  STALNCSSSLLYRILRFLVRRGIFKQEITEENVISYDHTPLSRLLASSNNNSMAPLLLLE 120

Query: 121 SNPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVP 180
           S+PVMLAPWH LSARIKG GETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARV TVP
Sbjct: 121 SSPVMLAPWHRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVHTVP 180

Query: 181 AILEDCPEIFEGDGSLVDVGGGNGTCLSMIVKACPWIKGINFDLPECHFCESSGFYM 238
           AILEDCP+IFEG GSLVDVGGGNGTCLSMIVKA PWIKGINFDLP  H   SS  Y+
Sbjct: 181 AILEDCPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLP--HVISSSQQYI 233

[Close]

BLAST of Bhi05G001433 vs. ExPASy TrEMBL
Match: A0A6J1GLX2 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455163 PE=3 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.6e-92
Identity = 179/237 (75.53%), Postives = 200/237 (84.39%), Query Frame = 0


Query: 1   MDFASKESIPNKEEEEEEVRVQIWKYIFGFVEMAIVKCAIELKIVDTIESHGSSMTLSQL 60
           MDFA K+SI +K+EE E  R+Q+W+YIFGFVEMAI+KCAIELKI DTI+SHG  MTL +L
Sbjct: 1   MDFALKQSI-SKKEEHEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPEL 60

Query: 61  SSALNYSFSLLYRIWRFLVPRGIFKQEITQENLTSYAQTPLSRLLASSSKNSMAPFLLLE 120
           SS+LN S SLLYRI RFLV RGIFK+EIT ENLT Y+ TPLSRLLASSS +SMAP LLLE
Sbjct: 61  SSSLNCSSSLLYRIMRFLVHRGIFKEEITDENLTCYSHTPLSRLLASSSDSSMAPLLLLE 120

Query: 121 SNPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVP 180
           SNPVMLAPWHGLSARIKG    PFEAAHGKD+WS+AAA+P HN + NDAM+C+ARV+TVP
Sbjct: 121 SNPVMLAPWHGLSARIKGNEAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMTVP 180

Query: 181 AILEDCPEIFEGDGSLVDVGGGNGTCLSMIVKACPWIKGINFDLPECHFCESSGFYM 238
           AILEDC EIFEG   LVDVGGGNGT LSMIVKACPWIKGINFDLP  H   SS  Y+
Sbjct: 181 AILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLP--HVVASSPPYI 234

[Close]

BLAST of Bhi05G001433 vs. ExPASy TrEMBL
Match: A0A6J1C5N1 ((R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111007607 PE=3 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 1.4e-91
Identity = 173/225 (76.89%), Postives = 187/225 (83.11%), Query Frame = 0


Query: 1   MDFASKESIPNKEEEEEEVRVQIWKYIFGFVEMAIVKCAIELKIVDTIESHGSSMTLSQL 60
           MD A KES   KEE+ E  RVQ+W YIFGF E+AI+KCAIELKI DTIESHGS MTLS+L
Sbjct: 11  MDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKL 70

Query: 61  SSALNYSFSLLYRIWRFLVPRGIFKQEITQENLTSYAQTPLSRLLASSSKNSMAPFLLLE 120
           SSALN S  LLYRI RFL  RGIFK+E T +NLT YAQTPLSRLLAS+S NSMAPFLLLE
Sbjct: 71  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLLE 130

Query: 121 SNPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVP 180
           SNPVMLAPWH LSA IK    TPFEA HGKD+WS+ AADP HN + NDAM+C ARV+TVP
Sbjct: 131 SNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 190

Query: 181 AILEDCPEIFEGDGSLVDVGGGNGTCLSMIVKACPWIKGINFDLP 226
           AILE C EIFEG GSLVDVGGGNGT LSMIVKACPWI+GINFDLP
Sbjct: 191 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLP 235

The following BLAST results are available for this feature:

BLAST of Bhi05G001433 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35160.1	6.3e-52	50.23	O-methyltransferase family protein	[more]
AT4G35150.1	3.3e-37	40.81	O-methyltransferase family protein	[more]
AT5G54160.1	1.5e-16	32.66	O-methyltransferase 1	[more]
AT1G77520.1	2.3e-14	29.61	O-methyltransferase family protein	[more]
AT5G53810.1	2.3e-14	29.54	O-methyltransferase family protein	[more]

BLAST of Bhi05G001433 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9T003	8.8e-51	50.23	Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1...	[more]
Q6WUC2	2.3e-35	40.00	(R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=...	[more]
B0ZB55	5.7e-34	40.19	Desmethylxanthohumol 6'-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT1 P...	[more]
B0ZB56	1.1e-32	39.66	Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1	[more]
B0ZB57	1.8e-32	39.91	Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1	[more]

BLAST of Bhi05G001433 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K2N4	1.4e-104	83.97	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G017720 PE=3 SV=1	[more]
A0A5D3DJ08	1.3e-100	83.12	(R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3BII4	1.3e-100	83.12	(R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490...	[more]
A0A6J1GLX2	1.6e-92	75.53	(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1C5N1	1.4e-91	76.89	(R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LO...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 129..226 e-value: 3.8E-13 score: 49.3
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 6..105 e-value: 1.3E-24 score: 87.7
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 106..232 e-value: 7.4E-30 score: 105.7
None	No IPR available	PANTHER	PTHR11746:SF191	3'-HYDROXY-N-METHYL-(S)-COCLAURINE 4'-O-METHYLTRANSFERASE 2-LIKE	coord: 11..227
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 35..79 e-value: 4.6E-9 score: 36.2
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 11..227
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 24..247 score: 34.210346
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 111..226
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 13..109

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi05M001433	Bhi05M001433	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019438	aromatic compound biosynthetic process
biological_process	GO:0032259	methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity