Bhi05G001419 (gene) Wax gourd (B227) v1

Overview


Name	Bhi05G001419
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	DDE Tnp4 domain-containing protein
Location	chr5: 54019985 .. 54020383 (-)
RNA-Seq Expression	Bhi05G001419
Synteny	Bhi05G001419

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GACTCCCTAACTGTTGTGGTTCGATTGATACCTACCACTCACATCACAATGTGTCTGCCTGCTTCGGATTCCACGAGTTATGTATGGCTTGACGAGGAGAAAAACCACAGCATGGTCTTGCAAGTGATTGTAGACGCTGAAATGATGTTCCGAGACATATTAACTGGCTTGCCTGGAAAAATGTCAGACTGGTCAGTTTTCCAGAGTTCAAACTTCCACAAGCTCTGTGACAAGGGGGAGAGGTTGAATGGGAAGAGGTTAGAGCTTAACGACAGATCGGAAATCAGAGAATATATAATTGGAGATTCAGGCTATCTTTTACTTCCCTATCTATTGAACACGGCGTTGATTACAAATAACAGGTTTGTGATGTTTTTGACTCAAAGGGTGCATATCTGA

mRNA sequence

GACTCCCTAACTGTTGTGGTTCGATTGATACCTACCACTCACATCACAATGTGTCTGCCTGCTTCGGATTCCACGAGTTATGTATGGCTTGACGAGGAGAAAAACCACAGCATGGTCTTGCAAGTGATTGTAGACGCTGAAATGATGTTCCGAGACATATTAACTGGCTTGCCTGGAAAAATGTCAGACTGGTCAGTTTTCCAGAGTTCAAACTTCCACAAGCTCTGTGACAAGGGGGAGAGGTTGAATGGGAAGAGGTTAGAGCTTAACGACAGATCGGAAATCAGAGAATATATAATTGGAGATTCAGGCTATCTTTTACTTCCCTATCTATTGAACACGGCGTTGATTACAAATAACAGGTTTGTGATGTTTTTGACTCAAAGGGTGCATATCTGA

Coding sequence (CDS)

GACTCCCTAACTGTTGTGGTTCGATTGATACCTACCACTCACATCACAATGTGTCTGCCTGCTTCGGATTCCACGAGTTATGTATGGCTTGACGAGGAGAAAAACCACAGCATGGTCTTGCAAGTGATTGTAGACGCTGAAATGATGTTCCGAGACATATTAACTGGCTTGCCTGGAAAAATGTCAGACTGGTCAGTTTTCCAGAGTTCAAACTTCCACAAGCTCTGTGACAAGGGGGAGAGGTTGAATGGGAAGAGGTTAGAGCTTAACGACAGATCGGAAATCAGAGAATATATAATTGGAGATTCAGGCTATCTTTTACTTCCCTATCTATTGAACACGGCGTTGATTACAAATAACAGGTTTGTGATGTTTTTGACTCAAAGGGTGCATATCTGA

Protein sequence

DSLTVVVRLIPTTHITMCLPASDSTSYVWLDEEKNHSMVLQVIVDAEMMFRDILTGLPGKMSDWSVFQSSNFHKLCDKGERLNGKRLELNDRSEIREYIIGDSGYLLLPYLLNTALITNNRFVMFLTQRVHI

Homology

[Close]

BLAST of Bhi05G001419 vs. TAIR 10
Match: AT3G55350.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 121.3 bits (303), Expect = 5.7e-28
Identity = 56/103 (54.37%), Postives = 79/103 (76.70%), Query Frame = 0


Query: 10  IPTTHITMCLPASDSTSYVWLDEEKNHSMVLQVIVDAEMMFRDILTGLPGKMSDWSVFQS 69
           I  THI M LPA + ++ VWLD EKN SM LQ +VD +M F D++ G PG ++D  V ++
Sbjct: 187 IDITHIVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGWPGSLNDDVVLKN 246

Query: 70  SNFHKLCDKGERLNGKRLELNDRSEIREYIIGDSGYLLLPYLL 113
           S F+KL +KG+RLNG++L L++R+E+REYI+GDSG+ LLP+LL
Sbjct: 247 SGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLL 289

[Close]

BLAST of Bhi05G001419 vs. TAIR 10
Match: AT3G63270.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: PIF / Ping-Pong family of plant transposases (TAIR:AT3G55350.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 88.6 bits (218), Expect = 4.1e-18
Identity = 46/103 (44.66%), Postives = 66/103 (64.08%), Query Frame = 0


Query: 10  IPTTHITMCLPASDSTSYVWLDEEKNHSMVLQVIVDAEMMFRDILTGLPGKMSDWSVFQS 69
           I TTHI M LPA  + S  W D+EKN+SM LQ + D EM F +++TG PG M+   + + 
Sbjct: 183 IDTTHIIMTLPAVQA-SDDWCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTVSKLLKF 242

Query: 70  SNFHKLCDKGERLNGKRLELNDRSEIREYIIGDSGYLLLPYLL 113
           S F KLC+  + L+G    L+  ++IREY++G   Y LLP+L+
Sbjct: 243 SGFFKLCENAQILDGNPKTLSQGAQIREYVVGGISYPLLPWLI 284

[Close]

BLAST of Bhi05G001419 vs. TAIR 10
Match: AT4G29780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12010.1); Has 945 Blast hits to 944 proteins in 87 species: Archae - 0; Bacteria - 0; Metazoa - 519; Fungi - 43; Plants - 365; Viruses - 0; Other Eukaryotes - 18 (source: NCBI BLink). )

HSP 1 Score: 43.9 bits (102), Expect = 1.2e-04
Identity = 32/112 (28.57%), Postives = 55/112 (49.11%), Query Frame = 0


Query: 6   VVRLIPTTHITMCLPASDSTSY-----VWLDEEKNHSMVLQVIVDAEMMFRDILTGLPGK 65
           VV  I TTHI +  P     +Y        +++ ++S+ +Q +V+A+ +F D+  G PG 
Sbjct: 323 VVGSIYTTHIPIIAPKVHVAAYFNKRHTERNQKTSYSITVQGVVNADGIFTDVCIGNPGS 382

Query: 66  MSDWSVFQSSNFHKLCDKGERLNGKRLELNDRSEIREYIIGDSGYLLLPYLL 113
           ++D  + + S+         R    R  L D      +I+G+SG+ L  YLL
Sbjct: 383 LTDDQILEKSSL-------SRQRAARGMLRD-----SWIVGNSGFPLTDYLL 422

[Close]

BLAST of Bhi05G001419 vs. TAIR 10
Match: AT3G19120.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 41.6 bits (96), Expect = 5.8e-04
Identity = 24/76 (31.58%), Postives = 41/76 (53.95%), Query Frame = 0


Query: 37  SMVLQVIVDAEMMFRDILTGLPGKMSDWSVFQSSNFHKLCDKGERLNGKRLELNDRSEIR 96
           +++LQV+ D + +F D+    PG   D S F+ S  +K    G+ +  K + +     +R
Sbjct: 255 AVLLQVVADHKKIFWDVCVKAPGGEDDSSHFRDSLLYKRLTSGDIVWEKVINIRGH-HVR 314

Query: 97  EYIIGDSGYLLLPYLL 113
            YI+GD  Y LL +L+
Sbjct: 315 PYIVGDWCYPLLSFLM 329

[Close]

BLAST of Bhi05G001419 vs. ExPASy Swiss-Prot
Match: Q9M2U3 (Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 8.1e-27
Identity = 56/103 (54.37%), Postives = 79/103 (76.70%), Query Frame = 0


Query: 10  IPTTHITMCLPASDSTSYVWLDEEKNHSMVLQVIVDAEMMFRDILTGLPGKMSDWSVFQS 69
           I  THI M LPA + ++ VWLD EKN SM LQ +VD +M F D++ G PG ++D  V ++
Sbjct: 187 IDITHIVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGWPGSLNDDVVLKN 246

Query: 70  SNFHKLCDKGERLNGKRLELNDRSEIREYIIGDSGYLLLPYLL 113
           S F+KL +KG+RLNG++L L++R+E+REYI+GDSG+ LLP+LL
Sbjct: 247 SGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLL 289

[Close]

BLAST of Bhi05G001419 vs. ExPASy Swiss-Prot
Match: Q94K49 (Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=ALP1 PE=1 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 5.8e-17
Identity = 46/103 (44.66%), Postives = 66/103 (64.08%), Query Frame = 0


Query: 10  IPTTHITMCLPASDSTSYVWLDEEKNHSMVLQVIVDAEMMFRDILTGLPGKMSDWSVFQS 69
           I TTHI M LPA  + S  W D+EKN+SM LQ + D EM F +++TG PG M+   + + 
Sbjct: 183 IDTTHIIMTLPAVQA-SDDWCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTVSKLLKF 242

Query: 70  SNFHKLCDKGERLNGKRLELNDRSEIREYIIGDSGYLLLPYLL 113
           S F KLC+  + L+G    L+  ++IREY++G   Y LLP+L+
Sbjct: 243 SGFFKLCENAQILDGNPKTLSQGAQIREYVVGGISYPLLPWLI 284

[Close]

BLAST of Bhi05G001419 vs. ExPASy TrEMBL
Match: A0A0A0M0C2 (DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G481730 PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.4e-45
Identity = 92/103 (89.32%), Postives = 96/103 (93.20%), Query Frame = 0


Query: 10  IPTTHITMCLPASDSTSYVWLDEEKNHSMVLQVIVDAEMMFRDILTGLPGKMSDWSVFQS 69
           I TTHITMCLPASD TSYVWLD++KNHSMVLQVIVDAEM FRDILTGLPGK+SDW VFQS
Sbjct: 174 IDTTHITMCLPASDPTSYVWLDDKKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQS 233

Query: 70  SNFHKLCDKGERLNGKRLELNDRSEIREYIIGDSGYLLLPYLL 113
           SNFHKLCDKGERLNGKR EL DRSEIREYIIGDSGY LLPYL+
Sbjct: 234 SNFHKLCDKGERLNGKRFELPDRSEIREYIIGDSGYPLLPYLV 276

[Close]

BLAST of Bhi05G001419 vs. ExPASy TrEMBL
Match: A0A5A7SXL0 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43059G001000 PE=3 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 6.4e-43
Identity = 89/103 (86.41%), Postives = 95/103 (92.23%), Query Frame = 0


Query: 10  IPTTHITMCLPASDSTSYVWLDEEKNHSMVLQVIVDAEMMFRDILTGLPGKMSDWSVFQS 69
           I TTHITMCLPASD TS+VWLD+EKNHSMVLQVIVDAEM FRDILTGLPGK+SD  VFQS
Sbjct: 174 IDTTHITMCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQS 233

Query: 70  SNFHKLCDKGERLNGKRLELNDRSEIREYIIGDSGYLLLPYLL 113
           SNFHKLCDKGERLNGKRLEL DRSEI+EYI+GDSGY LL YL+
Sbjct: 234 SNFHKLCDKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLV 276

[Close]

BLAST of Bhi05G001419 vs. ExPASy TrEMBL
Match: A0A5D3BNB9 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold49G00830 PE=3 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 6.4e-43
Identity = 89/103 (86.41%), Postives = 95/103 (92.23%), Query Frame = 0


Query: 10  IPTTHITMCLPASDSTSYVWLDEEKNHSMVLQVIVDAEMMFRDILTGLPGKMSDWSVFQS 69
           I TTHITMCLPASD TS+VWLD+EKNHSMVLQVIVDAEM FRDILTGLPGK+SD  VFQS
Sbjct: 174 IDTTHITMCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQS 233

Query: 70  SNFHKLCDKGERLNGKRLELNDRSEIREYIIGDSGYLLLPYLL 113
           SNFHKLCDKGERLNGKRLEL DRSEI+EYI+GDSGY LL YL+
Sbjct: 234 SNFHKLCDKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLV 276

[Close]

BLAST of Bhi05G001419 vs. ExPASy TrEMBL
Match: A0A6J1K643 (protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111492660 PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.9e-42
Identity = 88/103 (85.44%), Postives = 92/103 (89.32%), Query Frame = 0


Query: 10  IPTTHITMCLPASDSTSYVWLDEEKNHSMVLQVIVDAEMMFRDILTGLPGKMSDWSVFQS 69
           I TTHITMCLP  D TS VWLD EKNHSMVLQVIVDAEM FRDI+TGLPGKMSDW VFQS
Sbjct: 175 IDTTHITMCLPVLDPTSNVWLDAEKNHSMVLQVIVDAEMRFRDIITGLPGKMSDWLVFQS 234

Query: 70  SNFHKLCDKGERLNGKRLELNDRSEIREYIIGDSGYLLLPYLL 113
           SNFHKLC+KGERLNGKRLE  +RSEIREYIIGDSGY LLPYL+
Sbjct: 235 SNFHKLCEKGERLNGKRLEFINRSEIREYIIGDSGYPLLPYLV 277

[Close]

BLAST of Bhi05G001419 vs. ExPASy TrEMBL
Match: A0A6J1GBJ6 (protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111452471 PE=3 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 2.4e-42
Identity = 88/103 (85.44%), Postives = 92/103 (89.32%), Query Frame = 0


Query: 10  IPTTHITMCLPASDSTSYVWLDEEKNHSMVLQVIVDAEMMFRDILTGLPGKMSDWSVFQS 69
           I TTHITMCLP  D TS VWLD EKNHSMVLQVIVDAEM FRDI+TGLPGKMSDW VFQS
Sbjct: 175 IDTTHITMCLPVLDPTSNVWLDAEKNHSMVLQVIVDAEMRFRDIVTGLPGKMSDWLVFQS 234

Query: 70  SNFHKLCDKGERLNGKRLELNDRSEIREYIIGDSGYLLLPYLL 113
           SNFHKLC+KGERLNGKRLE  +RSEIREYIIGDSGY LLPYL+
Sbjct: 235 SNFHKLCEKGERLNGKRLEFINRSEIREYIIGDSGYPLLPYLV 277

The following BLAST results are available for this feature:

BLAST of Bhi05G001419 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G55350.1	5.7e-28	54.37	PIF / Ping-Pong family of plant transposases	[more]
AT3G63270.1	4.1e-18	44.66	CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int...	[more]
AT4G29780.1	1.2e-04	28.57	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT3G19120.1	5.8e-04	31.58	PIF / Ping-Pong family of plant transposases	[more]

BLAST of Bhi05G001419 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2U3	8.1e-27	54.37	Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1	[more]
Q94K49	5.8e-17	44.66	Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=...	[more]

BLAST of Bhi05G001419 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0M0C2	1.4e-45	89.32	DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G481730 PE...	[more]
A0A5A7SXL0	6.4e-43	86.41	Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]
A0A5D3BNB9	6.4e-43	86.41	Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A6J1K643	1.9e-42	85.44	protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111492660 PE=3 SV=1	[more]
A0A6J1GBJ6	2.4e-42	85.44	protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111452471 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027806	Harbinger transposase-derived nuclease domain	PFAM	PF13359	DDE_Tnp_4	coord: 12..110 e-value: 2.4E-9 score: 37.1
None	No IPR available	PANTHER	PTHR22930	UNCHARACTERIZED	coord: 9..113
None	No IPR available	PANTHER	PTHR22930:SF194	NUCLEASE HARBI1 ISOFORM X1-RELATED	coord: 9..113

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi05M001419	Bhi05M001419	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding