Bhi05G001416 (gene) Wax gourd (B227) v1

BLAST of Bhi05G001416 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 355.1 bits (910), Expect = 6.3e-98
Identity = 187/355 (52.68%), Postives = 246/355 (69.30%), Query Frame = 0

Query: 13  EEEGQARVQIWKYIFGFVEMAIVKCVIELKIVDTIESHGSS--MTLSQLSSALNCSSSLL 72
           +EE +A + IWKY+FGF ++A  KC I+LKI + IE+H SS  +TL++LSSA++ S S L
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPSHL 83

Query: 73  YRIMRFLVPRGIFKQEITQENL-TSYAQTPLSRLLASSSKN--SMAPFLLLESNPVMLAP 132
            RIMRFLV +GIFK+  T++ L T Y  TPLSR L  + ++  S+APF+L E+ P MLAP
Sbjct: 84  RRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEMLAP 143

Query: 133 WHGL----SARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPAILE 192
           W  L    S+ + G+   PF+A HGKDVWSFA  +P  + +IN+AM+C AR   VP +  
Sbjct: 144 WLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDAR-RVVPRVAG 203

Query: 193 DCPKIFEGIGSLVDVGGGNGTCLSMIVKTCPWIKGINFDLPHVISSSQEYVGVQHVGGNM 252
            C  +F+G+ ++VDVGGG G  + M+VK  PWIKG NFDLPHVI  ++   GV++V G+M
Sbjct: 204 ACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVENVEGDM 263

Query: 253 FDSIPKADAAFFMWVLHDWDDETCIKILKNCRDAIPEKTGKVIIVEAVIEE--------K 312
           FDSIP  DA F  WVLHDW D+ CIKILKNC++A+P   GKV+IVE+VI E        +
Sbjct: 264 FDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGENKKTMIVDE 323

Query: 313 EENNLSDVGLMLDMVMMAHTNNGKERTAKEWGYVLHQAGFTRYTITHIPVVQSVI 351
            +  L  V LMLDMVMMAHT+ GKERT KEW +VL +AGF RY +  I  VQS+I
Sbjct: 324 RDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQSLI 377

BLAST of Bhi05G001416 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 297.0 bits (759), Expect = 2.0e-80
Identity = 161/349 (46.13%), Postives = 216/349 (61.89%), Query Frame = 0

Query: 13  EEEGQARVQIWKYIFGFVEMAIVKCVIELKIVDTIESHGSS--MTLSQLSSALNCSSSLL 72
           +EE +A + IW+Y+FGF ++A  KC I+LKI + IE+H SS  +TLS+LSSA++ S S L
Sbjct: 10  DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASPSHL 69

Query: 73  YRIMRFLVPRGIFKQEITQENL-TSYAQTPLSRLLASSSKNSMAPFLLLESNPVMLAPWH 132
            RIMRFLV +G+FK+  T++ L T Y  TPLSR +  +                      
Sbjct: 70  RRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMMITK--------------------- 129

Query: 133 GLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPAILEDCPKIF 192
                            HGKD+W+FA  +  H+ +IN+AM+C AR   VP +   C  +F
Sbjct: 130 ----------------LHGKDLWAFAQDNLCHSQLINEAMACDAR-RVVPRVAGACQGLF 189

Query: 193 EGIGSLVDVGGGNGTCLSMIVKTCPWIKGINFDLPHVISSSQEYVGVQHVGGNMFDSIPK 252
           +G+ ++VDVGGG G  + ++VK  PWIKG NFDLPHVI  +Q   GV++V G+MFDSIP 
Sbjct: 190 DGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVEGDMFDSIPA 249

Query: 253 ADAAFFMWVLHDWDDETCIKILKNCRDAIPEKTGKVIIVEAVIEEKE--------ENNLS 312
           +DA    WVLHDW D+ CIKILKNC++A+    GKV+IVE VI EK+        ++ L 
Sbjct: 250 SDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKNTMIAEERDDKLE 309

Query: 313 DVGLMLDMVMMAHTNNGKERTAKEWGYVLHQAGFTRYTITHIPVVQSVI 351
            V L LDMVMM HT+ GKERT KEW +VL +AGF RY +     VQS+I
Sbjct: 310 HVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLI 320

BLAST of Bhi05G001416 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 198.4 bits (503), Expect = 9.9e-51
Identity = 116/311 (37.30%), Postives = 180/311 (57.88%), Query Frame = 0

Query: 35  VKCVIELKIVDTIESHGSSMTLSQLSSALNCSSS----LLYRIMRFLVPRGIF---KQEI 94
           +K  +EL +++ +  +GS M+ ++++S L   +     +L RI+R L    +     +++
Sbjct: 36  LKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNRKL 95

Query: 95  TQENLTS-YAQTPLSRLLASSSKN-SMAPFLLLESNPVMLAPWHGLSARIKGTGETPFEA 154
           + + +   Y   P+ + L  +    S+A   L+  + V++  W+ L   I   G  PF  
Sbjct: 96  SGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAIL-DGGIPFNK 155

Query: 155 AHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPAILEDCPKIFEGIGSLVDVGGGNGTC 214
           A+G   + +   DP  N V N+ MS     +T+  ILE   K FEG+ SLVDVGGG G  
Sbjct: 156 AYGMSAFEYHGTDPRFNKVFNNGMS-NHSTITMKKILETY-KGFEGLTSLVDVGGGIGAT 215

Query: 215 LSMIVKTCPWIKGINFDLPHVISSSQEYVGVQHVGGNMFDSIPKADAAFFMWVLHDWDDE 274
           L MIV   P +KGINFDLPHVI  +  + G++HVGG+MF S+PK DA F  W+ HDW DE
Sbjct: 216 LKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDE 275

Query: 275 TCIKILKNCRDAIPEKTGKVIIVEAVIEEKEENNLSDVGLM-LDMVMMAHTNNGKERTAK 334
            C+K LKNC +++PE  GKVI+ E ++ E  +++LS   ++ +D +M+AH   GKERT K
Sbjct: 276 HCVKFLKNCYESLPE-DGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKERTEK 335

Query: 335 EWGYVLHQAGF 336
           E+  +   +GF
Sbjct: 336 EFEALAKASGF 342

BLAST of Bhi05G001416 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 180.6 bits (457), Expect = 2.1e-45
Identity = 119/338 (35.21%), Postives = 175/338 (51.78%), Query Frame = 0

Query: 34  IVKCVIELKIVDTIESHGSSMTLSQLSSALNCSSS--------LLYRIMRFLVPRGIFKQ 93
           ++K   EL ++DTI + G+   LS    A +  +         LL R++  LV   I K 
Sbjct: 45  VLKAAFELGVIDTIAAAGNDTWLSPCEIACSLPTKPTNPEAPVLLDRMLSLLVSHSILKC 104

Query: 94  EI--TQENLTS------YAQTPLSR--LLASSSKNSMAPFLLLESNPVMLAPWHGLSARI 153
            +  T EN  +      YA  P+ +  L  S    S+ P  +L    V    W  L   I
Sbjct: 105 RMIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGSLVPLFMLLHTQVFFKTWTNLKDVI 164

Query: 154 KGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPAILEDCPKIFEGIGSL 213
              G   F +AHG  ++ +  +D     + N AMS  + ++    +  D  + FE + +L
Sbjct: 165 L-EGRDAFNSAHGMKIFEYINSDQPFAELFNRAMSEPSTMIMKKVL--DVYRGFEDVNTL 224

Query: 214 VDVGGGNGTCLSMIVKTCPWIKGINFDLPHVISSSQEYVGVQHVGGNMFDSIPKADAAFF 273
           VDVGGGNGT L ++    P IKG+NFDL  V++ +  Y GV+HV G+MF  +PK DA F 
Sbjct: 225 VDVGGGNGTVLGLVTSKYPHIKGVNFDLAQVLTQAPFYPGVEHVSGDMFVEVPKGDAVFM 284

Query: 274 MWVLHDWDDETCIKILKNCRDAIPEKTGKVIIVEAVI--EEKEENNLSDVGLMLDMVMMA 333
            W+LHDW DE CIKILKNC  ++PEK GK+IIVE V   E K  +  S+    +D++M+ 
Sbjct: 285 KWILHDWGDEDCIKILKNCWKSLPEK-GKIIIVEFVTPKEPKGGDLSSNTVFAMDLLMLT 344

Query: 334 HTNNGKERTAKEWGYVLHQAGFTRYTITHIPVVQSVIQ 352
             + GKER+  ++  +   +GF R  I  +    SVI+
Sbjct: 345 QCSGGKERSLSQFENLAFASGFLRCEIICLAYSYSVIE 378

BLAST of Bhi05G001416 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 176.8 bits (447), Expect = 3.1e-44
Identity = 125/369 (33.88%), Postives = 197/369 (53.39%), Query Frame = 0

Query: 6   KESLPKKE---EEGQARVQIWKYIFGFVEMAIVKCVIELKIVDTI--ESHGSSMTLSQLS 65
           K  L K+E   +E    +Q    +       ++K  +EL ++DTI   S+G+ ++ S+++
Sbjct: 14  KPGLTKEEQEIDEKMVSLQAESIVNAVAFPMVLKAALELGVIDTIAAASNGTWLSPSEIA 73

Query: 66  SALNCSSS------LLYRIMRFLVPRGIFK---QEITQENLTS-----YAQTPLSR--LL 125
            +L    +      LL R++R LV   I K    E  +   T      YA  P+ +  L 
Sbjct: 74  VSLPNKPTNPEAPVLLDRMLRLLVSHSILKCCMVESRENGQTGKIERVYAAEPICKYFLK 133

Query: 126 ASSSKNSMAPFLLLESNPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIV 185
            S    S++  LLL  + V+L  W  L   I   G+  F +AH   ++ + ++D   + +
Sbjct: 134 DSDGSGSLSSLLLLLHSQVILKTWTNLKDVIL-EGKDAFSSAHDMRLFEYISSDDQFSKL 193

Query: 186 INDAMSCTARVLTVPAILEDCPKIFEGIGSLVDVGGGNGTCLSMIVKTCPWIKGINFDLP 245
            + AMS  +  + +  +LE+  + FE + +LVDVGGG GT L +I    P IKG+NFDL 
Sbjct: 194 FHRAMS-ESSTMVMKKVLEEY-RGFEDVNTLVDVGGGIGTILGLITSKYPHIKGVNFDLA 253

Query: 246 HVISSSQEYVGVQHVGGNMFDSIPKADAAFFMWVLHDWDDETCIKILKNCRDAIPEKTGK 305
            V++ +  Y GV+HV G+MF  +PK DA F  W+LHDW DE CIKILKNC  ++PEK GK
Sbjct: 254 QVLTQAPFYPGVKHVSGDMFIEVPKGDAIFMKWILHDWGDEDCIKILKNCWKSLPEK-GK 313

Query: 306 VIIVEAV--IEEKEENNLSDVGLMLDMVMMAHTNNGKERTAKEWGYVLHQAGFTRYTITH 352
           VIIVE +  +E K  +   +  L +D++M+   + GKER+  ++  +   +GF    I  
Sbjct: 314 VIIVEMITPMEPKPNDFSCNTVLGMDLLMLTQCSGGKERSLSQFENLAFASGFLLCEIIC 373

BLAST of Bhi05G001416 vs. ExPASy Swiss-Prot
Match: Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 8.9e-97
Identity = 187/355 (52.68%), Postives = 246/355 (69.30%), Query Frame = 0

Query: 13  EEEGQARVQIWKYIFGFVEMAIVKCVIELKIVDTIESHGSS--MTLSQLSSALNCSSSLL 72
           +EE +A + IWKY+FGF ++A  KC I+LKI + IE+H SS  +TL++LSSA++ S S L
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPSHL 83

Query: 73  YRIMRFLVPRGIFKQEITQENL-TSYAQTPLSRLLASSSKN--SMAPFLLLESNPVMLAP 132
            RIMRFLV +GIFK+  T++ L T Y  TPLSR L  + ++  S+APF+L E+ P MLAP
Sbjct: 84  RRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEMLAP 143

Query: 133 WHGL----SARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPAILE 192
           W  L    S+ + G+   PF+A HGKDVWSFA  +P  + +IN+AM+C AR   VP +  
Sbjct: 144 WLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDAR-RVVPRVAG 203

Query: 193 DCPKIFEGIGSLVDVGGGNGTCLSMIVKTCPWIKGINFDLPHVISSSQEYVGVQHVGGNM 252
            C  +F+G+ ++VDVGGG G  + M+VK  PWIKG NFDLPHVI  ++   GV++V G+M
Sbjct: 204 ACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVENVEGDM 263

Query: 253 FDSIPKADAAFFMWVLHDWDDETCIKILKNCRDAIPEKTGKVIIVEAVIEE--------K 312
           FDSIP  DA F  WVLHDW D+ CIKILKNC++A+P   GKV+IVE+VI E        +
Sbjct: 264 FDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGENKKTMIVDE 323

Query: 313 EENNLSDVGLMLDMVMMAHTNNGKERTAKEWGYVLHQAGFTRYTITHIPVVQSVI 351
            +  L  V LMLDMVMMAHT+ GKERT KEW +VL +AGF RY +  I  VQS+I
Sbjct: 324 RDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQSLI 377

BLAST of Bhi05G001416 vs. ExPASy Swiss-Prot
Match: Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 4.3e-75
Identity = 153/351 (43.59%), Postives = 214/351 (60.97%), Query Frame = 0

Query: 13  EEEGQARVQIWKYIFGFVEMAIVKCVIELKIVDTIESHGSSMTLSQLSSALNCSS----- 72
           EE  + + +IW+++F FV+   +KC +EL I D I SHG  +T+S++  +L  ++     
Sbjct: 5   EERLKGQAEIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSSP 64

Query: 73  --SLLYRIMRFLVPRGIFKQEITQE-NLTSYAQTPLSRLLASSSKNSMAPFLLLESNPVM 132
               L RIMR LV + +F  E+ QE N   Y  T  S+ L   SK +++P +L E+NP++
Sbjct: 65  NIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPIL 124

Query: 133 LAPWHGLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPAILED 192
           L PW  L  +      +PFE AHG ++W  A ADP  N  +N AM C+   +    +LE 
Sbjct: 125 LKPWQYL-GKCAQEKSSPFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEMLLE- 184

Query: 193 CPKIFEGI-GSLVDVGGGNGTCLSMIVKTCPWIKGINFDLPHVISSSQEYVGVQHVGGNM 252
               F GI GSLVDVGGG G+ ++ IVK  P I+GINFDLPHV++++ E+ GV+HVGG+M
Sbjct: 185 YKDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVGGDM 244

Query: 253 FDSIPKADAAFFMWVLHDWDDETCIKILKNCRDAI-PEKTGKVIIVEAVIEEKEENNLSD 312
           F  IP+ADA    W+LHDW DE C  ILKNC  AI  +K GKVIIV+ V+     +    
Sbjct: 245 FVDIPEADAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVLRPDGNDLFDK 304

Query: 313 VGLMLDMVMMAHTNNGKERTAKEWGYVLHQAGFTRYTITHIPVVQSVIQGF 354
           +GL+ D++MMAHT  GKERT  EW  +L+ AGF RY +   P    +I+ F
Sbjct: 305 MGLIFDVLMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIEAF 353

BLAST of Bhi05G001416 vs. ExPASy Swiss-Prot
Match: B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 3.6e-74
Identity = 159/348 (45.69%), Postives = 217/348 (62.36%), Query Frame = 0

Query: 17  QARVQIWKYIFGFVEMAIVKCVIELKIVDTIESHGSSMTLSQLSSALNCSSSL----LYR 76
           +    +WK I G  +  ++KC +EL+I D + SH + +TL+Q++S++  S SL    L R
Sbjct: 14  RGEANVWKSINGIADFMVMKCALELRIPDIVHSHSAPITLAQIASSVPDSPSLNLSYLSR 73

Query: 77  IMRFLVPRGIFKQEITQE-NLTSYAQTPLSRLLASSS----KNSMAPFLLLESNPVMLAP 136
           IMR LV R IF Q  + +     Y  T  SRLL S +    + ++APF+   ++P + AP
Sbjct: 74  IMRLLVRRKIFSQHKSLDGEEVLYGPTHSSRLLLSKTTLPDQVTLAPFVAFMTHPYLSAP 133

Query: 137 WHGLSARIKGTGETPFEAAH-GKDVWSFAAADPIHNIVINDAMSCTARVLTVPAILEDCP 196
           W  L+  +K  G   FE  H G+ +W  +  +P  N V ND M+ TAR+ T+ AIL +  
Sbjct: 134 WSCLARCVK-EGGNGFEMVHGGRQLWDLSPGNPEFNKVFNDGMASTARITTM-AILSEYR 193

Query: 197 KIFEGIGSLVDVGGGNGTCLSMIVKTCPWIKGINFDLPHVISSSQEYVG-VQHVGGNMFD 256
            +F GI SLVDVGG  G  +S IVK+ P IKGIN+DLPHV++++  Y G V HVGGNMF+
Sbjct: 194 DVFCGICSLVDVGGEFGGSISAIVKSHPHIKGINYDLPHVVATAPTYTGLVSHVGGNMFE 253

Query: 257 SIPKADAAFFMWVLHDWDDETCIKILKNCRDAIPEKTGKVIIVEAVIEEKEENNLSDVGL 316
            IP A A F  W+LHDW DE C+KILKNCR A+PEK GK+IIV+ V+E +      D  +
Sbjct: 254 WIPTAVAVFMKWILHDWADEDCVKILKNCRRAMPEKGGKIIIVDIVLEPEGNGLFDDAAV 313

Query: 317 MLDMVMMAHTNNGKERTAKEWGYVLHQAGFTRYTITHIPVVQSVIQGF 354
           MLD+ +MA T  GKERT KEW  VL + GF RY I  IP + SVI+ +
Sbjct: 314 MLDIALMALT-RGKERTEKEWKRVLEEGGFPRYQILKIPALTSVIEAY 358

BLAST of Bhi05G001416 vs. ExPASy Swiss-Prot
Match: Q9LEL5 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 8.7e-68
Identity = 135/342 (39.47%), Postives = 207/342 (60.53%), Query Frame = 0

Query: 17  QARVQIWKYIFGFVEMAIVKCVIELKIVDTIESHGSSMTLSQLSSAL-----NCSSSLLY 76
           +A+  +WK I+GF +  +++C +EL IVD I+++   M L+ L+S L     NC +  LY
Sbjct: 13  KAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSDVNCDN--LY 72

Query: 77  RIMRFLVPRGIFKQEITQENLTSYAQTPLSRLLASSSKNSMAPFLLLESNPVMLAPWHGL 136
           RI+R+LV   I + E + +    YA  P++ LL+ ++K SM P +L  +    + PWH +
Sbjct: 73  RILRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNAKRSMVPMILGMTQKDFMTPWHSM 132

Query: 137 SARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPAILEDCPKIFEG 196
              +   G T FE A G  +W +    P  + + N+ M+   R+LT  +++     +F+G
Sbjct: 133 KDGLSDNG-TAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLT-SSLISGSRDMFQG 192

Query: 197 IGSLVDVGGGNGTCLSMIVKTCPWIKGINFDLPHVISSSQEYVGVQHVGGNMFDSIPKAD 256
           I SLVDVGGGNGT +  I    P IK   FDLPHVI++S +   ++ +GG+MF S+P A 
Sbjct: 193 IDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDMFKSVPSAQ 252

Query: 257 AAFFMWVLHDWDDETCIKILKNCRDAIPEKTGKVIIVEAVIEEKEENNLSDVGLMLDMVM 316
           A     +LHDW+DE  IKILK CR+A+P+  GKVIIV+  ++E+ ++ LS   L+LD+ M
Sbjct: 253 AIILKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSSTRLILDIDM 312

Query: 317 MAHTNNGKERTAKEWGYVLHQAGFTRYTITHIPVVQSVIQGF 354
           + +T  GKERT + W  ++  AGF+   I HI  +QSVI+ F
Sbjct: 313 LVNT-GGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVF 349

BLAST of Bhi05G001416 vs. ExPASy Swiss-Prot
Match: Q9LEL6 ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.9e-67
Identity = 139/345 (40.29%), Postives = 206/345 (59.71%), Query Frame = 0

Query: 12  KEEEGQARVQIWKYIFGFVEMAIVKCVIELKIVDTIESHGSSMTLSQLSSAL---NCSSS 71
           K++   ++ ++W +I+GF E  ++KC ++L + + I + G+SMTLS+LSS L     +  
Sbjct: 4   KKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPVNED 63

Query: 72  LLYRIMRFLVPRGIFKQEITQENLTSYAQTPLSRLLASSSKNSMAPFLLLESNPVMLAPW 131
            LYR+MR+LV   +F +      L  Y   P ++ L       M   +L  ++   +APW
Sbjct: 64  ALYRVMRYLVHMKLFTKASIDGEL-RYGLAPPAKYLVKGWDKCMVGSILAITDKDFMAPW 123

Query: 132 HGLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPAILEDCPKI 191
           H L   + G   T FE A G ++W + A  P  N + N+AM+  +R L + A++++C  I
Sbjct: 124 HYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSR-LIMSALVKECGNI 183

Query: 192 FEGIGSLVDVGGGNGTCLSMIVKTCPWIKGINFDLPHVISSSQEYVGVQHVGGNMFDSIP 251
           F GI +LVDVGGG GT +  I    P IK   +DLPHVI+ S  Y  V  V G+MF  IP
Sbjct: 184 FNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFIP 243

Query: 252 KADAAFFMWVLHDWDDETCIKILKNCRDAIPEKTGKVIIVEAVIEEKEENNLSDVGLMLD 311
           KADA     +LHDWDD+ CI+ILK C++A+P K GKVIIV+ V+  + E+  + + L LD
Sbjct: 244 KADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMRLTLD 303

Query: 312 MVMMAHTNNGKERTAKEWGYVLHQAGFTRYTITHIPVVQSVIQGF 354
           + MM +T  GKERT +EW  ++H AG+  + IT I  VQSVI+ +
Sbjct: 304 LDMMLNT-GGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAY 345

BLAST of Bhi05G001416 vs. ExPASy TrEMBL
Match: A0A5D3DJ08 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004240 PE=3 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 4.5e-168
Identity = 302/358 (84.36%), Postives = 321/358 (89.66%), Query Frame = 0

Query: 1   MDFVSKESLPKKEEEGQARVQIWKYIFGFVEMAIVKCVIELKIVDTIESHGSSMTLSQLS 60
           M+F  + S+ KKEEE +ARVQIWKYIFGFVEMAIVKC IEL+I DTIESHGS MTLSQLS
Sbjct: 1   MNFAPQVSISKKEEE-EARVQIWKYIFGFVEMAIVKCAIELRIGDTIESHGSPMTLSQLS 60

Query: 61  SALNCSSSLLYRIMRFLVPRGIFKQEITQENLTSYAQTPLSRLLASSSKNSMAPFLLLES 120
           +ALNCSSSLLYRI+RFLV RGIFKQEIT+EN+ SY  TPLSRLLASS+ NSMAP LLLES
Sbjct: 61  TALNCSSSLLYRILRFLVRRGIFKQEITEENVISYDHTPLSRLLASSNNNSMAPLLLLES 120

Query: 121 NPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPA 180
           +PVMLAPWH LSARIKG GETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARV TVPA
Sbjct: 121 SPVMLAPWHRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVHTVPA 180

Query: 181 ILEDCPKIFEGIGSLVDVGGGNGTCLSMIVKTCPWIKGINFDLPHVISSSQEYVGVQHVG 240
           ILEDCP+IFEGIGSLVDVGGGNGTCLSMIVK  PWIKGINFDLPHVISSSQ+Y+GV+HVG
Sbjct: 181 ILEDCPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQYIGVEHVG 240

Query: 241 GNMFDSIPKADAAFFMWVLHDWDDETCIKILKNCRDAIPEKTGKVIIVEAVIEEKEE--- 300
           GNM DSIPKADAAF MWVLHDWDDETCIKILKNC++AI EK GKVIIVEA+IEE+ E   
Sbjct: 241 GNMLDSIPKADAAFIMWVLHDWDDETCIKILKNCKEAISEKRGKVIIVEAIIEERSEEEN 300

Query: 301 --NNLSDVGLMLDMVMMAHTNNGKERTAKEWGYVLHQAGFTRYTITHIPVVQSVIQGF 354
             NNL DVGLMLDMVMMAHT NGKERT KEWG VLHQAGFTRYTIT I  V SVIQGF
Sbjct: 301 NNNNLGDVGLMLDMVMMAHTENGKERTIKEWGNVLHQAGFTRYTITPIRAVHSVIQGF 357

BLAST of Bhi05G001416 vs. ExPASy TrEMBL
Match: A0A0A0K2N4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G017720 PE=3 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 4.5e-168
Identity = 299/358 (83.52%), Postives = 324/358 (90.50%), Query Frame = 0

Query: 1   MDFVSKESLPKK-EEEGQARVQIWKYIFGFVEMAIVKCVIELKIVDTIESHGSSMTLSQL 60
           MDF  ++S+PKK EEE +ARV+IWKYIFGFVEMAIVKC IEL+I DTIESHGS MTLSQL
Sbjct: 1   MDFAPQKSIPKKQEEEEEARVEIWKYIFGFVEMAIVKCAIELRIGDTIESHGSPMTLSQL 60

Query: 61  SSALNCSSSLLYRIMRFLVPRGIFKQEITQENLTSYAQTPLSRLLASSSKNSMAPFLLLE 120
           S+ALNCS+SLLYRI+RFLV RGIFKQEI + N+ SY QTPLSRLLASS+ NSMAPFLLLE
Sbjct: 61  STALNCSASLLYRILRFLVRRGIFKQEINEANVISYDQTPLSRLLASSNNNSMAPFLLLE 120

Query: 121 SNPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVP 180
           S+PVMLAPWH LSARIKG GETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVP
Sbjct: 121 SSPVMLAPWHRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVP 180

Query: 181 AILEDCPKIFEGIGSLVDVGGGNGTCLSMIVKTCPWIKGINFDLPHVISSSQEYVGVQHV 240
           AILE+CP+IFEGIGSLVDVGGGNGTCLSMIVK  PWIKGINFDLPHVISSSQ+Y+GV+HV
Sbjct: 181 AILEECPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQYIGVEHV 240

Query: 241 GGNMFDSIPKADAAFFMWVLHDWDDETCIKILKNCRDAIPEKTGKVIIVEAVIEEKEE-- 300
           GGNM DSIPKADAAF MWVLHDWDDETCIKILKNC+ AI EK GKVIIVEA+IEE+ E  
Sbjct: 241 GGNMLDSIPKADAAFIMWVLHDWDDETCIKILKNCKGAISEKRGKVIIVEALIEERSEEN 300

Query: 301 -NNLSDVGLMLDMVMMAHTNNGKERTAKEWGYVLHQAGFTRYTITHIPVVQSVIQGFI 355
            N L DVGLMLDMVMMAHT NGKERT+KEWG+VLHQAGFT+YTIT I  V SVIQ F+
Sbjct: 301 NNKLGDVGLMLDMVMMAHTKNGKERTSKEWGHVLHQAGFTQYTITPIRAVHSVIQAFL 358

BLAST of Bhi05G001416 vs. ExPASy TrEMBL
Match: A0A1S3BII4 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490252 PE=3 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 2.2e-167
Identity = 301/359 (83.84%), Postives = 321/359 (89.42%), Query Frame = 0

Query: 1   MDFVSKESLPKKEEEGQARVQIWKYIFGFVEMAIVKCVIELKIVDTIESHGSSMTLSQLS 60
           M+F  + S+ KKEEE +ARVQIWKYIFGFVEMAIVKC IEL+I DTIESHGS MTLSQLS
Sbjct: 1   MNFAPQVSISKKEEE-EARVQIWKYIFGFVEMAIVKCAIELRIGDTIESHGSPMTLSQLS 60

Query: 61  SALNCSSSLLYRIMRFLVPRGIFKQEITQENLTSYAQTPLSRLLASSSKNSMAPFLLLES 120
           +ALNCSSSLLYRI+RFLV RGIFKQEIT+EN+ SY  TPLSRLLASS+ NSMAP LLLES
Sbjct: 61  TALNCSSSLLYRILRFLVRRGIFKQEITEENVISYDHTPLSRLLASSNNNSMAPLLLLES 120

Query: 121 NPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPA 180
           +PVMLAPWH LSARIKG GETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARV TVPA
Sbjct: 121 SPVMLAPWHRLSARIKGNGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVHTVPA 180

Query: 181 ILEDCPKIFEGIGSLVDVGGGNGTCLSMIVKTCPWIKGINFDLPHVISSSQEYVGVQHVG 240
           ILEDCP+IFEGIGSLVDVGGGNGTCLSMIVK  PWIKGINFDLPHVISSSQ+Y+GV+HVG
Sbjct: 181 ILEDCPQIFEGIGSLVDVGGGNGTCLSMIVKAFPWIKGINFDLPHVISSSQQYIGVEHVG 240

Query: 241 GNMFDSIPKADAAFFMWVLHDWDDETCIKILKNCRDAIPEKTGKVIIVEAVIEEKEE--- 300
           GNM DSIPKADAAF MWVLHDWDDETCIKILKNC++AI EK GKVIIVEA+IEE+ E   
Sbjct: 241 GNMLDSIPKADAAFIMWVLHDWDDETCIKILKNCKEAISEKRGKVIIVEAIIEERSEEEN 300

Query: 301 --NNLSDVGLMLDMVMMAHTNNGKERTAKEWGYVLHQAGFTRYTITHIPVVQSVIQGFI 355
             NNL DVGLMLDMVMMAHT NGKERT KEWG VL QAGFTRYTIT I  V SVIQGF+
Sbjct: 301 NNNNLGDVGLMLDMVMMAHTENGKERTIKEWGNVLRQAGFTRYTITPIRAVHSVIQGFL 358

BLAST of Bhi05G001416 vs. ExPASy TrEMBL
Match: A0A6J1GLX2 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455163 PE=3 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 4.8e-162
Identity = 282/353 (79.89%), Postives = 313/353 (88.67%), Query Frame = 0

Query: 1   MDFVSKESLPKKEEEGQARVQIWKYIFGFVEMAIVKCVIELKIVDTIESHGSSMTLSQLS 60
           MDF  K+S+ KKEE   AR+Q+W+YIFGFVEMAI+KC IELKI DTI+SHG  MTL +LS
Sbjct: 1   MDFALKQSISKKEEHEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPELS 60

Query: 61  SALNCSSSLLYRIMRFLVPRGIFKQEITQENLTSYAQTPLSRLLASSSKNSMAPFLLLES 120
           S+LNCSSSLLYRIMRFLV RGIFK+EIT ENLT Y+ TPLSRLLASSS +SMAP LLLES
Sbjct: 61  SSLNCSSSLLYRIMRFLVHRGIFKEEITDENLTCYSHTPLSRLLASSSDSSMAPLLLLES 120

Query: 121 NPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPA 180
           NPVMLAPWHGLSARIKG    PFEAAHGKD+WS+AAA+P HN + NDAM+C+ARV+TVPA
Sbjct: 121 NPVMLAPWHGLSARIKGNEAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMTVPA 180

Query: 181 ILEDCPKIFEGIGSLVDVGGGNGTCLSMIVKTCPWIKGINFDLPHVISSSQEYVGVQHVG 240
           ILEDC +IFEG+  LVDVGGGNGT LSMIVK CPWIKGINFDLPHV++SS  Y+GVQHVG
Sbjct: 181 ILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVVASSPPYIGVQHVG 240

Query: 241 GNMFDSIPKADAAFFMWVLHDWDDETCIKILKNCRDAIPEKTGKVIIVEAVIEEKEENNL 300
           GNMFD IPKADAAF MWVLH WDDETCIKILK CR+AIP+KTGKVIIVEAV+EEKEENNL
Sbjct: 241 GNMFDCIPKADAAFLMWVLHLWDDETCIKILKKCREAIPKKTGKVIIVEAVLEEKEENNL 300

Query: 301 SDVGLMLDMVMMAHTNNGKERTAKEWGYVLHQAGFTRYTITHIPVVQSVIQGF 354
           SDVGLMLDMVMMAHTN+GKERTA+EW YVLHQAGFTR+TIT I  +QSVIQ F
Sbjct: 301 SDVGLMLDMVMMAHTNDGKERTAEEWAYVLHQAGFTRHTITPIRALQSVIQAF 353

BLAST of Bhi05G001416 vs. ExPASy TrEMBL
Match: A0A6J1GLX9 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111455166 PE=3 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 1.0e-156
Identity = 277/353 (78.47%), Postives = 308/353 (87.25%), Query Frame = 0

Query: 1   MDFVSKESLPKKEEEGQARVQIWKYIFGFVEMAIVKCVIELKIVDTIESHGSSMTLSQLS 60
           MDF  K+S+ KKEE   AR+Q+W+YIFGFVEMAI+KC IELKI DTI+SHG  MTL +LS
Sbjct: 1   MDFALKQSISKKEEHEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPELS 60

Query: 61  SALNCSSSLLYRIMRFLVPRGIFKQEITQENLTSYAQTPLSRLLASSSKNSMAPFLLLES 120
           S+LNCSSSLLYRIMRFLV RGIFK+E T+     YAQTP+SRLLASSS +SMAP LL ES
Sbjct: 61  SSLNCSSSLLYRIMRFLVHRGIFKEEPTK----GYAQTPMSRLLASSSGSSMAPLLLFES 120

Query: 121 NPVMLAPWHGLSARIKGTGETPFEAAHGKDVWSFAAADPIHNIVINDAMSCTARVLTVPA 180
           NPVMLAPWHGLSARIKG    PFEAAHGKD+WS+AAA+P HN + NDAM+C+ARV++VPA
Sbjct: 121 NPVMLAPWHGLSARIKGNEAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMSVPA 180

Query: 181 ILEDCPKIFEGIGSLVDVGGGNGTCLSMIVKTCPWIKGINFDLPHVISSSQEYVGVQHVG 240
           ILEDC +IFEG+  LVDVGGGNGT LSMIVK CPWIK INFDLPHVI+S    + VQHVG
Sbjct: 181 ILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKAINFDLPHVIASLPADIDVQHVG 240

Query: 241 GNMFDSIPKADAAFFMWVLHDWDDETCIKILKNCRDAIPEKTGKVIIVEAVIEEKEENNL 300
           GNMFDSIPKADAAF MWVLHDWDDETCIKILKNCR+AIP+KTGKVIIVEAV+EEKEENNL
Sbjct: 241 GNMFDSIPKADAAFLMWVLHDWDDETCIKILKNCREAIPKKTGKVIIVEAVLEEKEENNL 300

Query: 301 SDVGLMLDMVMMAHTNNGKERTAKEWGYVLHQAGFTRYTITHIPVVQSVIQGF 354
           SDVGLMLDMVMMAHTN+GKERTAKEW YVLHQAGFTR+TIT I  +QSVIQ F
Sbjct: 301 SDVGLMLDMVMMAHTNDGKERTAKEWAYVLHQAGFTRHTITPIRALQSVIQAF 349