Bhi05G001245 (gene) Wax gourd (B227) v1

Overview


Name	Bhi05G001245
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Calcium-binding EF-hand family protein
Location	chr5: 49770054 .. 49770467 (-)
RNA-Seq Expression	Bhi05G001245
Synteny	Bhi05G001245

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTGTGGAAATATTGGATAGTACCACCATTGTCAACTTTGTTGAAGATGAGGCAGCTTTTAATGCCTACATAAGAGACCGATTCGATCACCTCGACACTAACCATGATGGTCACCTTTCCTATAATGAGATGCTGGAGGAGTTGCAAAGCCTCAGGGTGTTTGAGACCGATTTCGGAAAGGATGAGAAGCTTGATCCAGAAGAGCTTTCTTCTGTCTATAGTTCTCTGTTTTTGCAGTTCGATCGTGACTCAGACGGAACGGTTGATCTGGATGAATTTAAGACCGAGACTAAAAGGATGATGCTAGCTATGGCTAATGGGATTGGTTTCTTACCCGTTCAGATGGTGCTTGAAGAGGGCAGCTTCTTGATGAAGGCTGTTGAGCGTGAAACTGCAATAATGGCCGCTTAA

mRNA sequence

ATGAGTGTGGAAATATTGGATAGTACCACCATTGTCAACTTTGTTGAAGATGAGGCAGCTTTTAATGCCTACATAAGAGACCGATTCGATCACCTCGACACTAACCATGATGGTCACCTTTCCTATAATGAGATGCTGGAGGAGTTGCAAAGCCTCAGGGTGTTTGAGACCGATTTCGGAAAGGATGAGAAGCTTGATCCAGAAGAGCTTTCTTCTGTCTATAGTTCTCTGTTTTTGCAGTTCGATCGTGACTCAGACGGAACGGTTGATCTGGATGAATTTAAGACCGAGACTAAAAGGATGATGCTAGCTATGGCTAATGGGATTGGTTTCTTACCCGTTCAGATGGTGCTTGAAGAGGGCAGCTTCTTGATGAAGGCTGTTGAGCGTGAAACTGCAATAATGGCCGCTTAA

Coding sequence (CDS)

ATGAGTGTGGAAATATTGGATAGTACCACCATTGTCAACTTTGTTGAAGATGAGGCAGCTTTTAATGCCTACATAAGAGACCGATTCGATCACCTCGACACTAACCATGATGGTCACCTTTCCTATAATGAGATGCTGGAGGAGTTGCAAAGCCTCAGGGTGTTTGAGACCGATTTCGGAAAGGATGAGAAGCTTGATCCAGAAGAGCTTTCTTCTGTCTATAGTTCTCTGTTTTTGCAGTTCGATCGTGACTCAGACGGAACGGTTGATCTGGATGAATTTAAGACCGAGACTAAAAGGATGATGCTAGCTATGGCTAATGGGATTGGTTTCTTACCCGTTCAGATGGTGCTTGAAGAGGGCAGCTTCTTGATGAAGGCTGTTGAGCGTGAAACTGCAATAATGGCCGCTTAA

Protein sequence

MSVEILDSTTIVNFVEDEAAFNAYIRDRFDHLDTNHDGHLSYNEMLEELQSLRVFETDFGKDEKLDPEELSSVYSSLFLQFDRDSDGTVDLDEFKTETKRMMLAMANGIGFLPVQMVLEEGSFLMKAVERETAIMAA

Homology

[Close]

BLAST of Bhi05G001245 vs. TAIR 10
Match: AT2G44310.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 110.2 bits (274), Expect = 1.4e-24
Identity = 55/135 (40.74%), Postives = 92/135 (68.15%), Query Frame = 0


Query: 1   MSVEILDSTTIVNFVEDEAAFNAYIRDRFDHLDTNHDGHLSYNEMLEELQSLRVFETDFG 60
           M V ++D +T+ +FV+DE  F   + +RF  LD N DG LS +E+ +  +S+R+ E+ FG
Sbjct: 1   MGVVLIDGSTVRSFVDDEEQFKKSVDERFAALDLNKDGVLSRSELRKAFESMRLLESHFG 60

Query: 61  KDEKLDPEELSSVYSSLFLQFDRDSDGTVDLDEFKTETKRMMLAMANGIGFLPVQMVL-- 120
            D     +EL+++Y S+F +FD D  G+VDL+EF++E K+++LA+A+G+G  P+ MVL  
Sbjct: 61  VDVVTPQDELTNLYDSIFEKFDTDQSGSVDLEEFRSEMKKIVLAIADGLGSCPITMVLDD 120

Query: 121 EEGSFLMKAVERETA 134
           ++ +FL KA + E +
Sbjct: 121 DDDNFLKKAADLEAS 135

[Close]

BLAST of Bhi05G001245 vs. TAIR 10
Match: AT1G54530.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 57.4 bits (137), Expect = 1.1e-08
Identity = 37/128 (28.91%), Postives = 67/128 (52.34%), Query Frame = 0


Query: 5   ILDSTTIVNFVEDEAAFNAYIRDRFDHLDTNHDGHLSYNEMLEELQSLRVFETDFGKDEK 64
           ++ S T++ F+ D  +F +   D F  LD + +G LS +E+ + L ++   E++    ++
Sbjct: 5   VITSKTLIGFLSDTKSFESITNDYFQILDLDKNGMLSPSELRQGLNNVVAVESEVAPGDE 64

Query: 65  LDPEELSSVYSSLFLQFDRDSDGTVDLDEFKTETKRMMLAMANGIGFLPVQMVLEEGSFL 124
            D     +VY+++F +F  D    +    F+     ++ AMA GIG  PV MV+     +
Sbjct: 65  TD-----NVYNAIFERFGED----LVPKNFRDLIAEILTAMARGIGNSPVIMVVHNDGLI 123

Query: 125 MKAVERET 133
           MKAV  E+
Sbjct: 125 MKAVLHES 123

[Close]

BLAST of Bhi05G001245 vs. TAIR 10
Match: AT4G38810.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 50.4 bits (119), Expect = 1.3e-06
Identity = 33/125 (26.40%), Postives = 58/125 (46.40%), Query Frame = 0


Query: 4   EILDSTTIVNFVEDEAAFNAYIRDRFDHLDTNHDGHLSYNEMLEELQSLRVFETDFGKDE 63
           ++LD + IV  VE+E  F+ ++  +F  LD + DG LS  E+   +  +       G   
Sbjct: 13  QVLDGSDIVELVENEKVFDKFVEQKFQQLDQDEDGKLSVTELQPAVADI---GAALGLPA 72

Query: 64  KLDPEELSSVYSSLFLQFDRDSDGTVDLDEFKTETKRMMLAMANGIGFLPVQMVLEEGSF 123
           +    +   +YS +  +F   S   V   EFK     ++L MA G+   P+ ++  +G  
Sbjct: 73  QGTSPDSDHIYSEVLNEFTHGSQEKVSKTEFKEVLSDILLGMAAGLKRDPIVILRMDGED 132

Query: 124 LMKAV 129
           L + V
Sbjct: 133 LSEFV 134

[Close]

BLAST of Bhi05G001245 vs. ExPASy TrEMBL
Match: A0A5A7UT84 (Calcium-binding EF-hand family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold150G00150 PE=4 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 1.1e-61
Identity = 127/137 (92.70%), Postives = 130/137 (94.89%), Query Frame = 0


Query: 1   MSVEILDSTTIVNFVEDEAAFNAYIRDRFDHLDTNHDGHLSYNEMLEELQSLRVFETDFG 60
           MSVEILDSTTIVNFVEDEAAFNAYIRDRF HLDTNHDG LSYNEMLEELQSLRVFE DFG
Sbjct: 1   MSVEILDSTTIVNFVEDEAAFNAYIRDRFTHLDTNHDGLLSYNEMLEELQSLRVFEADFG 60

Query: 61  KDEKLDPEELSSVYSSLFLQFDRDSDGTVDLDEFKTETKRMMLAMANGIGFLPVQMVLEE 120
            D+KLDP+ELSSVYSSLFLQFDRDSDG VDLD +KTE KRMMLAMANGIGFLPVQMVLEE
Sbjct: 61  NDKKLDPDELSSVYSSLFLQFDRDSDGMVDLDAYKTEIKRMMLAMANGIGFLPVQMVLEE 120

Query: 121 GSFLMKAVERETAIMAA 138
           GSFLMKAVERETAIMAA
Sbjct: 121 GSFLMKAVERETAIMAA 137

[Close]

BLAST of Bhi05G001245 vs. ExPASy TrEMBL
Match: A0A1S3C175 (uncharacterized protein LOC103495653 OS=Cucumis melo OX=3656 GN=LOC103495653 PE=4 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 1.1e-61
Identity = 127/137 (92.70%), Postives = 130/137 (94.89%), Query Frame = 0


Query: 1   MSVEILDSTTIVNFVEDEAAFNAYIRDRFDHLDTNHDGHLSYNEMLEELQSLRVFETDFG 60
           MSVEILDSTTIVNFVEDEAAFNAYIRDRF HLDTNHDG LSYNEMLEELQSLRVFE DFG
Sbjct: 1   MSVEILDSTTIVNFVEDEAAFNAYIRDRFTHLDTNHDGLLSYNEMLEELQSLRVFEADFG 60

Query: 61  KDEKLDPEELSSVYSSLFLQFDRDSDGTVDLDEFKTETKRMMLAMANGIGFLPVQMVLEE 120
            D+KLDP+ELSSVYSSLFLQFDRDSDG VDLD +KTE KRMMLAMANGIGFLPVQMVLEE
Sbjct: 61  NDKKLDPDELSSVYSSLFLQFDRDSDGMVDLDAYKTEIKRMMLAMANGIGFLPVQMVLEE 120

Query: 121 GSFLMKAVERETAIMAA 138
           GSFLMKAVERETAIMAA
Sbjct: 121 GSFLMKAVERETAIMAA 137

[Close]

BLAST of Bhi05G001245 vs. ExPASy TrEMBL
Match: A0A0A0K3B2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G037625 PE=4 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.9e-58
Identity = 122/137 (89.05%), Postives = 127/137 (92.70%), Query Frame = 0


Query: 1   MSVEILDSTTIVNFVEDEAAFNAYIRDRFDHLDTNHDGHLSYNEMLEELQSLRVFETDFG 60
           MSVEILD TTIVNFVEDEAAFNAYIR +F HLDT+HDG LSYNEMLEELQSLRVF+ DFG
Sbjct: 1   MSVEILDGTTIVNFVEDEAAFNAYIRAQFAHLDTDHDGLLSYNEMLEELQSLRVFDADFG 60

Query: 61  KDEKLDPEELSSVYSSLFLQFDRDSDGTVDLDEFKTETKRMMLAMANGIGFLPVQMVLEE 120
            DEKL P+ELSSVYSSLFLQFDRDSDG VDLD +KTE KRMMLAMANGIGFLPVQMVLEE
Sbjct: 61  NDEKLGPDELSSVYSSLFLQFDRDSDGMVDLDAYKTEIKRMMLAMANGIGFLPVQMVLEE 120

Query: 121 GSFLMKAVERETAIMAA 138
           GSFLMKAVERETAIMAA
Sbjct: 121 GSFLMKAVERETAIMAA 137

[Close]

BLAST of Bhi05G001245 vs. ExPASy TrEMBL
Match: A0A5D3E445 (Calcium-binding EF-hand family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G00260 PE=4 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 5.8e-55
Identity = 117/137 (85.40%), Postives = 122/137 (89.05%), Query Frame = 0


Query: 1   MSVEILDSTTIVNFVEDEAAFNAYIRDRFDHLDTNHDGHLSYNEMLEELQSLRVFETDFG 60
           MSVEILDS TIVNFVED+ AF A+IRDRF HLDTNHDG LSY+EMLEEL +LRVFET FG
Sbjct: 1   MSVEILDSATIVNFVEDDVAFGAFIRDRFAHLDTNHDGLLSYSEMLEELHTLRVFETHFG 60

Query: 61  KDEKLDPEELSSVYSSLFLQFDRDSDGTVDLDEFKTETKRMMLAMANGIGFLPVQMVLEE 120
            D K DPEELSSVYSSLFLQFD DS GTVDLDEFK ETKRMML MANG+GFLPVQMVLEE
Sbjct: 61  IDVKPDPEELSSVYSSLFLQFDHDSSGTVDLDEFKAETKRMMLDMANGMGFLPVQMVLEE 120

Query: 121 GSFLMKAVERETAIMAA 138
           GSFLMKAVERETAI AA
Sbjct: 121 GSFLMKAVERETAITAA 137

[Close]

BLAST of Bhi05G001245 vs. ExPASy TrEMBL
Match: A0A1S3BLY5 (uncharacterized protein LOC103491294 OS=Cucumis melo OX=3656 GN=LOC103491294 PE=4 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 5.8e-55
Identity = 117/137 (85.40%), Postives = 122/137 (89.05%), Query Frame = 0


Query: 1   MSVEILDSTTIVNFVEDEAAFNAYIRDRFDHLDTNHDGHLSYNEMLEELQSLRVFETDFG 60
           MSVEILDS TIVNFVED+ AF A+IRDRF HLDTNHDG LSY+EMLEEL +LRVFET FG
Sbjct: 1   MSVEILDSATIVNFVEDDVAFGAFIRDRFAHLDTNHDGLLSYSEMLEELHTLRVFETHFG 60

Query: 61  KDEKLDPEELSSVYSSLFLQFDRDSDGTVDLDEFKTETKRMMLAMANGIGFLPVQMVLEE 120
            D K DPEELSSVYSSLFLQFD DS GTVDLDEFK ETKRMML MANG+GFLPVQMVLEE
Sbjct: 61  IDVKPDPEELSSVYSSLFLQFDHDSSGTVDLDEFKAETKRMMLDMANGMGFLPVQMVLEE 120

Query: 121 GSFLMKAVERETAIMAA 138
           GSFLMKAVERETAI AA
Sbjct: 121 GSFLMKAVERETAITAA 137

The following BLAST results are available for this feature:

BLAST of Bhi05G001245 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44310.1	1.4e-24	40.74	Calcium-binding EF-hand family protein	[more]
AT1G54530.1	1.1e-08	28.91	Calcium-binding EF hand family protein	[more]
AT4G38810.2	1.3e-06	26.40	Calcium-binding EF-hand family protein	[more]

BLAST of Bhi05G001245 vs. TAIR 10


Match Name	E-value	Identity	Description

BLAST of Bhi05G001245 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UT84	1.1e-61	92.70	Calcium-binding EF-hand family protein OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3C175	1.1e-61	92.70	uncharacterized protein LOC103495653 OS=Cucumis melo OX=3656 GN=LOC103495653 PE=...	[more]
A0A0A0K3B2	1.9e-58	89.05	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G037625 PE=4 SV=1	[more]
A0A5D3E445	5.8e-55	85.40	Calcium-binding EF-hand family protein OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BLY5	5.8e-55	85.40	uncharacterized protein LOC103491294 OS=Cucumis melo OX=3656 GN=LOC103491294 PE=...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 73..101 e-value: 2.1 score: 15.1 coord: 24..52 e-value: 0.18 score: 20.9
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 25..96 e-value: 7.5E-12 score: 45.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 20..55 score: 10.329096
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 69..104 score: 10.050141
None	No IPR available	GENE3D	1.10.238.10		coord: 17..104 e-value: 7.9E-14 score: 53.5
None	No IPR available	PANTHER	PTHR34574	CALCIUM-BINDING EF-HAND FAMILY PROTEIN-RELATED	coord: 1..133
None	No IPR available	PANTHER	PTHR34574:SF10	CALCIUM-BINDING EF-HAND FAMILY PROTEIN-LIKE	coord: 1..133
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 33..45
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 82..94
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 24..102

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi05M001245	Bhi05M001245	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding