Bhi05G001131 (gene) Wax gourd (B227) v1

Overview
NameBhi05G001131
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionEndoglucanase
Locationchr5: 45516703 .. 45517383 (-)
RNA-Seq ExpressionBhi05G001131
SyntenyBhi05G001131
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTCGAAGAATCTAAAGAGCAAGTCGATGCTCTTACTCCTGAAGACATTGCTTGGGTTGATTCTTGTCTGATTAAAGAGACACCAGATATTTCAGATGGCAATTGGAACCACATAAAGGATGCCTTATTAGAAATCCTTGACCTGTATCCTCAAAGTTTTGAATCTTCTCATGCTGTGAGCCATAATGCTCCAGGAGGTAGTAATGGCGACATCGACGTTGACATGCTTTCCCCTAACAATGTGAAGGAGCCTACATTTTCCTCGAGAGATAGTGATGATCCTATGAATGAAACAGGAATAGCTTCGGAAGATCCACAAAAGCACGACGACATTGATACTTCTCTGTTACAAACTCTTATCAAGAATCCATTTTTACCCACTTACAAAGAGGAGGTAGAAGGGAATGATGAGAATAATCAAGCTGGATTTGAGCATGAATTATCAGAAATTGGATCTGAGTCCCCAATTAATGATATTTTCCATGTTTGGGATTTGAACTTCCCACCAGTCGAAGATGAGCTCGTTGAGCAGCTGAACAAAGCCCTTACCGAAAATTATGTTGAATCGACCCCGTCAATGGATGGTAATCTTCTTGTGTTGAAAGACTTGAAGGAAGATTTACTTGATGACTTGATCAATAGCATTTCTGACTTATCTTTGGAACAGACTAAAAACTAG

mRNA sequence

ATGGTCGAAGAATCTAAAGAGCAAGTCGATGCTCTTACTCCTGAAGACATTGCTTGGGTTGATTCTTGTCTGATTAAAGAGACACCAGATATTTCAGATGGCAATTGGAACCACATAAAGGATGCCTTATTAGAAATCCTTGACCTGTATCCTCAAAGTTTTGAATCTTCTCATGCTGTGAGCCATAATGCTCCAGGAGGTAGTAATGGCGACATCGACGTTGACATGCTTTCCCCTAACAATGTGAAGGAGCCTACATTTTCCTCGAGAGATAGTGATGATCCTATGAATGAAACAGGAATAGCTTCGGAAGATCCACAAAAGCACGACGACATTGATACTTCTCTGTTACAAACTCTTATCAAGAATCCATTTTTACCCACTTACAAAGAGGAGGTAGAAGGGAATGATGAGAATAATCAAGCTGGATTTGAGCATGAATTATCAGAAATTGGATCTGAGTCCCCAATTAATGATATTTTCCATGTTTGGGATTTGAACTTCCCACCAGTCGAAGATGAGCTCGTTGAGCAGCTGAACAAAGCCCTTACCGAAAATTATGTTGAATCGACCCCGTCAATGGATGGTAATCTTCTTGTGTTGAAAGACTTGAAGGAAGATTTACTTGATGACTTGATCAATAGCATTTCTGACTTATCTTTGGAACAGACTAAAAACTAG

Coding sequence (CDS)

ATGGTCGAAGAATCTAAAGAGCAAGTCGATGCTCTTACTCCTGAAGACATTGCTTGGGTTGATTCTTGTCTGATTAAAGAGACACCAGATATTTCAGATGGCAATTGGAACCACATAAAGGATGCCTTATTAGAAATCCTTGACCTGTATCCTCAAAGTTTTGAATCTTCTCATGCTGTGAGCCATAATGCTCCAGGAGGTAGTAATGGCGACATCGACGTTGACATGCTTTCCCCTAACAATGTGAAGGAGCCTACATTTTCCTCGAGAGATAGTGATGATCCTATGAATGAAACAGGAATAGCTTCGGAAGATCCACAAAAGCACGACGACATTGATACTTCTCTGTTACAAACTCTTATCAAGAATCCATTTTTACCCACTTACAAAGAGGAGGTAGAAGGGAATGATGAGAATAATCAAGCTGGATTTGAGCATGAATTATCAGAAATTGGATCTGAGTCCCCAATTAATGATATTTTCCATGTTTGGGATTTGAACTTCCCACCAGTCGAAGATGAGCTCGTTGAGCAGCTGAACAAAGCCCTTACCGAAAATTATGTTGAATCGACCCCGTCAATGGATGGTAATCTTCTTGTGTTGAAAGACTTGAAGGAAGATTTACTTGATGACTTGATCAATAGCATTTCTGACTTATCTTTGGAACAGACTAAAAACTAG

Protein sequence

MVEESKEQVDALTPEDIAWVDSCLIKETPDISDGNWNHIKDALLEILDLYPQSFESSHAVSHNAPGGSNGDIDVDMLSPNNVKEPTFSSRDSDDPMNETGIASEDPQKHDDIDTSLLQTLIKNPFLPTYKEEVEGNDENNQAGFEHELSEIGSESPINDIFHVWDLNFPPVEDELVEQLNKALTENYVESTPSMDGNLLVLKDLKEDLLDDLINSISDLSLEQTKN
Homology
BLAST of Bhi05G001131 vs. TAIR 10
Match: AT4G38980.1 (unknown protein; Has 44 Blast hits to 44 proteins in 19 species: Archae - 0; Bacteria - 2; Metazoa - 2; Fungi - 8; Plants - 24; Viruses - 0; Other Eukaryotes - 8 (source: NCBI BLink). )

HSP 1 Score: 62.8 bits (151), Expect = 4.2e-10
Identity = 73/281 (25.98%), Postives = 116/281 (41.28%), Query Frame = 0

Query: 2   VEESKEQVDALTPEDIAWVDSCLIKETPDISDGNWNHIKDALLEILDLYPQSF------- 61
           V  S    + L+PE +AW DSC+I    D  + NW   +DAL EI+D++P+ F       
Sbjct: 10  VSLSVRNEEDLSPECVAWADSCIISFLDDSDNNNWGTFRDALTEIIDIHPEMFVFSSTTG 69

Query: 62  ----------------------ESSHAVSHNAPGGSNGDID------------------- 121
                                 E +   +++    SN ++                    
Sbjct: 70  TRGVLSPDEVMTESETIDLRRSEPAAGSANSRTNSSNEEVSEIISMLTFESDPSKNCLED 129

Query: 122 ---VDMLSPNNVKEPTFSSR---DSDDPMNETGIASEDPQKHDDIDTSLLQTLIKNPFLP 181
               + ++ N  +EP   S+      + + E G  S    K ++   S+   + K+ F+ 
Sbjct: 130 YYFPESIAENGNREPADGSKTDLGGVESIEEDGSVSNGEAK-EEKPASVSSQVFKDDFMS 189

Query: 182 TYKEEVEGNDENNQAGFEHELSEIGSESPINDIFHVWDLNF---PPVEDELVEQLNKALT 225
           TY   VE N E      +  ++E   +    +IF VWDL        ED LV QL KAL 
Sbjct: 190 TY---VEDNAE------DCNVTEDPVKVTSQEIFKVWDLEVVGDNDEEDGLVLQLKKALD 249

BLAST of Bhi05G001131 vs. ExPASy TrEMBL
Match: A0A5D3BCZ2 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold10G00320 PE=4 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 5.5e-103
Identity = 199/234 (85.04%), Postives = 204/234 (87.18%), Query Frame = 0

Query: 4   ESKEQVDALTPEDIAWVDSCLIKETPDISDGNWNHIKDALLEILDLYPQSFESSHAVSHN 63
           ESKEQVD LT EDIAWVDSCLIKE PDISDGNWNH+KDALLEILDLYPQ FESS A+S N
Sbjct: 15  ESKEQVDGLTREDIAWVDSCLIKEIPDISDGNWNHVKDALLEILDLYPQGFESSLALSDN 74

Query: 64  APGGSNGDIDVDMLSPNNVKEPTFSSRDSDD------------PMNETGIASEDPQKHDD 123
            PG SNGDIDVDML  NNVKEPTFSSRDSDD            PMN+TGIASEDPQKHDD
Sbjct: 75  VPGASNGDIDVDMLPSNNVKEPTFSSRDSDDLMNETRTALEDHPMNDTGIASEDPQKHDD 134

Query: 124 IDTSLLQTLIKNPFLPTYKEEVEGNDENNQAGFEHELSEIGSESPINDIFHVWDLNFPPV 183
           IDTSL  TLIKNPFLPTYKEEVEGNDEN+QAG  HELSEIGSESPINDIFHVWDLNFPPV
Sbjct: 135 IDTSLPLTLIKNPFLPTYKEEVEGNDENDQAGIGHELSEIGSESPINDIFHVWDLNFPPV 194

Query: 184 EDELVEQLNKALTENYVESTPSMDGNLLVLKDLKEDLLDDLINSISDLSLEQTK 226
           EDEL+EQLNKALTEN VES PSMD NL VLKDLKEDLLDDLINSISDLSLEQTK
Sbjct: 195 EDELMEQLNKALTENSVESVPSMDSNLGVLKDLKEDLLDDLINSISDLSLEQTK 248

BLAST of Bhi05G001131 vs. ExPASy TrEMBL
Match: A0A1S3BBI4 (uncharacterized protein LOC103488123 OS=Cucumis melo OX=3656 GN=LOC103488123 PE=4 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 5.5e-103
Identity = 199/234 (85.04%), Postives = 204/234 (87.18%), Query Frame = 0

Query: 4   ESKEQVDALTPEDIAWVDSCLIKETPDISDGNWNHIKDALLEILDLYPQSFESSHAVSHN 63
           ESKEQVD LT EDIAWVDSCLIKE PDISDGNWNH+KDALLEILDLYPQ FESS A+S N
Sbjct: 15  ESKEQVDGLTREDIAWVDSCLIKEIPDISDGNWNHVKDALLEILDLYPQGFESSLALSDN 74

Query: 64  APGGSNGDIDVDMLSPNNVKEPTFSSRDSDD------------PMNETGIASEDPQKHDD 123
            PG SNGDIDVDML  NNVKEPTFSSRDSDD            PMN+TGIASEDPQKHDD
Sbjct: 75  VPGASNGDIDVDMLPSNNVKEPTFSSRDSDDLMNETRTALEDHPMNDTGIASEDPQKHDD 134

Query: 124 IDTSLLQTLIKNPFLPTYKEEVEGNDENNQAGFEHELSEIGSESPINDIFHVWDLNFPPV 183
           IDTSL  TLIKNPFLPTYKEEVEGNDEN+QAG  HELSEIGSESPINDIFHVWDLNFPPV
Sbjct: 135 IDTSLPLTLIKNPFLPTYKEEVEGNDENDQAGIGHELSEIGSESPINDIFHVWDLNFPPV 194

Query: 184 EDELVEQLNKALTENYVESTPSMDGNLLVLKDLKEDLLDDLINSISDLSLEQTK 226
           EDEL+EQLNKALTEN VES PSMD NL VLKDLKEDLLDDLINSISDLSLEQTK
Sbjct: 195 EDELMEQLNKALTENSVESVPSMDSNLGVLKDLKEDLLDDLINSISDLSLEQTK 248

BLAST of Bhi05G001131 vs. ExPASy TrEMBL
Match: A0A0A0K2C8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G006240 PE=4 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 4.1e-98
Identity = 194/235 (82.55%), Postives = 200/235 (85.11%), Query Frame = 0

Query: 4   ESKEQVDALTPEDIAWVDSCLIKETPDISDGNWNHIKDALLEILDLYPQSFESSHAVSHN 63
           ESKEQVD LTPEDIAWVDSCLIKE PDISDGNWN IKDALLEI+DLYPQ FESS A+S N
Sbjct: 15  ESKEQVDGLTPEDIAWVDSCLIKEVPDISDGNWNDIKDALLEIIDLYPQGFESSLALSDN 74

Query: 64  APGGSNGDIDVDMLSPNNVKEPTFSSRDSDD------------PMNETGIASEDPQ-KHD 123
            PG SNGDIDVDML  NNVKE TFSSRDSDD            PMN+TGIASEDPQ  HD
Sbjct: 75  VPGASNGDIDVDMLPSNNVKELTFSSRDSDDLMNETRMVPEDHPMNDTGIASEDPQMHHD 134

Query: 124 DIDTSLLQTLIKNPFLPTYKEEVEGNDENNQAGFEHELSEIGSESPINDIFHVWDLNFPP 183
           DIDTSL  TL+KNPFLPTYKEEVEGNDENNQAG  HELSEIGS+SPIN+IFHVWDLNFPP
Sbjct: 135 DIDTSLPFTLVKNPFLPTYKEEVEGNDENNQAGIGHELSEIGSDSPINNIFHVWDLNFPP 194

Query: 184 VEDELVEQLNKALTENYVESTPSMDGNLLVLKDLKEDLLDDLINSISDLSLEQTK 226
           VEDELVEQLNKALTEN VE  PSMD NL V KDLKEDLLDDLINSISDLSLEQTK
Sbjct: 195 VEDELVEQLNKALTENSVELVPSMDSNLGVSKDLKEDLLDDLINSISDLSLEQTK 249

BLAST of Bhi05G001131 vs. ExPASy TrEMBL
Match: A0A6J1C3A0 (uncharacterized protein LOC111007546 OS=Momordica charantia OX=3673 GN=LOC111007546 PE=4 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 4.0e-77
Identity = 158/220 (71.82%), Postives = 173/220 (78.64%), Query Frame = 0

Query: 4   ESKEQVDALTPEDIAWVDSCLIKETPDISDGNWNHIKDALLEILDLYPQSFESSHAVSHN 63
           ESKE VDALTPEDIAWVDSCLI ETPDISDGNWN++K ALLEILDLYP+SF SS  +S N
Sbjct: 15  ESKEPVDALTPEDIAWVDSCLINETPDISDGNWNNMKSALLEILDLYPESFASSAVLSDN 74

Query: 64  APGGSNGDIDVDMLSPNNVKEPTFSSRDSDDPMNETGIASEDPQKHDDIDTSLLQTLIKN 123
           APGG+  DI  DML  NN KE  F   D DDPM+E GIASEDP+ HDDIDTSL  +  KN
Sbjct: 75  APGGTKDDI--DMLPSNNEKESIFPWGDDDDPMSEPGIASEDPRDHDDIDTSLSLSFSKN 134

Query: 124 PFLPTYKEEVEGNDENNQAGFEHELSEIGSESPINDIFHVWDLNFPPVEDELVEQLNKAL 183
           PFLPTYKE+V G  EN Q G   +LSEIG E P+NDIF VWDLN PPVEDEL+EQLNKAL
Sbjct: 135 PFLPTYKEDV-GGKENTQTGSSQDLSEIGYEFPVNDIFRVWDLNLPPVEDELLEQLNKAL 194

Query: 184 TENYVESTPSMDGNLLVLKDLKEDLLDDLINSISDLSLEQ 224
           +EN  +S PSM  N  VLKD KE+ LDDLINSISDLSLEQ
Sbjct: 195 SENSFKSLPSMWDNRSVLKDFKEESLDDLINSISDLSLEQ 231

BLAST of Bhi05G001131 vs. ExPASy TrEMBL
Match: A0A6J1I1I4 (uncharacterized protein LOC111470037 OS=Cucurbita maxima OX=3661 GN=LOC111470037 PE=4 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 1.7e-72
Identity = 158/279 (56.63%), Postives = 175/279 (62.72%), Query Frame = 0

Query: 3   EESKEQVDALTPEDIAWVDSCLIKETPDISDGNWNHIKDALLEILDLYPQSFESSHAVSH 62
           +ESKEQ+D LTPEDIAW DSCLIKE PDI DGNWNHIKDALLE+ DLYPQ FES  AVS 
Sbjct: 14  QESKEQIDDLTPEDIAWADSCLIKEIPDILDGNWNHIKDALLEVFDLYPQGFESPLAVSD 73

Query: 63  NAPGGSNGDIDVDMLS-------------------------------------------- 122
           N PGG+N D+DVDML                                             
Sbjct: 74  NVPGGTNDDVDVDMLRFKNVKKSTERDSDDPMNDVDSSLSLSFSTNPLLPTYKEEDNVPG 133

Query: 123 ------------PNNVKEPTFSSRDSDDPMNETGIASEDPQKHDDIDTSLLQTLIKNPFL 182
                        NNVK+PTF SRDSDD MNETG+ASED Q HDDIDTSL  +  KNPFL
Sbjct: 134 GTSDDININMLLSNNVKKPTFFSRDSDDRMNETGMASEDQQHHDDIDTSLSLSFNKNPFL 193

Query: 183 PTYKEEVEGNDENNQAGFEHELSEIGSESPINDIFHVWDLNFPPVEDELVEQLNKALTEN 226
           PTYKEEV+G  E+ Q    H+L EIG E PINDIF VWDLN PP+E++LVEQLNKAL+EN
Sbjct: 194 PTYKEEVDGK-ESIQTESSHDLPEIGFEPPINDIFQVWDLNLPPIENDLVEQLNKALSEN 253

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G38980.14.2e-1025.98unknown protein; Has 44 Blast hits to 44 proteins in 19 species: Archae - 0; Bac... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3BCZ25.5e-10385.04Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3BBI45.5e-10385.04uncharacterized protein LOC103488123 OS=Cucumis melo OX=3656 GN=LOC103488123 PE=... [more]
A0A0A0K2C84.1e-9882.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G006240 PE=4 SV=1[more]
A0A6J1C3A04.0e-7771.82uncharacterized protein LOC111007546 OS=Momordica charantia OX=3673 GN=LOC111007... [more]
A0A6J1I1I41.7e-7256.63uncharacterized protein LOC111470037 OS=Cucurbita maxima OX=3661 GN=LOC111470037... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 57..109
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 81..96
NoneNo IPR availablePANTHERPTHR36388OS02G0469000 PROTEINcoord: 4..225

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi05M001131Bhi05M001131mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
molecular_function GO:0016787 hydrolase activity