Bhi04G002073 (gene) Wax gourd (B227) v1

Overview


Name	Bhi04G002073
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Disease resistance TIR-NBS-LRR class protein
Location	chr4: 68741884 .. 68744383 (-)
RNA-Seq Expression	Bhi04G002073
Synteny	Bhi04G002073

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGCTCGTGGTGAGAGCCTCATTGACAGGTACAGCTGCAAACTTTTGGCCTGCCATAAGTATGAGATGAAGTTTCTTTTCATCTATTTCTAGGTGTTGGAGGCCTGCATATGTATTAGAAGTTAACATAGGCGAGTGTGAATGAAATTCATGAGAAGCTTTATATATATATATATGTTAGACGATATATAATTTGGTTAAAATATCATTTTGGTCTTTGTACTTTGATAATTGTTCCATTTAAGTCTCTATACTTTCAAATGTCTAATTTTGGTCTCTGTCTTTTCAATAAAGCTTAAAATTGGTTCCCACCGATAAATTAAACTTAAAAATAAGTTGTGGTTGCAATAAAAAAAGGATGAAATAGGATAGAAAATTAAAAGTCAAGACACTTGGATTAAAAAATCAGTTTTTTTTTCCTTTCCCTTCCATCTTTTTCTTCTGCAACTTCATCTTCTCCGTGCTTTCTATCTTCCCCTTCTCCAACTTCACTTTCTTTCTTTCCCCATTTATTTGTAAATCTAGTCATGATTTTTTAAAATCTGCAAGTATAGCAAAAAATCTCAGCCTGATTTCTATTTTTCTGTTTTTTGAACTTTATTCTTGTTGGTGTATCAATAATATACTTAATATTCTTCATACTTTTTTTTAATAAATTCTTTTAATTTCATATATATACATATATTAGATATATAGCTGGTACGCATATGAAGATAAACTTTGTATAATTTATAAACGGAAATCAAGTTAATTTTTTTGCTATACTCACAAAAAAAAAGTTATTTTCAAATATAGGAAATGAGCTAACTTATTTAGAAATATAGCAAAATGTTTCATTGATAGACATCTATCAGTGTCTATCATTGATACCGTCTATCAATGTGTATTATGTCTATCATTAATAGACTGTGTTAGGACCCTCCTAACAAGAACAGTACTCAAGAATATAGGATACACTCAAAGGCAATTTAATTATAGAAATATAGGAAGATGTATTGAAATACGCTATAAACTTACGAACAAAATAACAAGATAAACCCTAGCCTTTTGAGAGGGCTAGTCTCTCCCAAATTCCCTAAAGGAAATTCCCACCAAATTCTCCCCACTCTCCCTCCAATCCCTCTCCTTCTATTTATAACTAAAATCCCTAACAAACTTCATATCTAATTACTCAGTTGTCCCTTCTAATACTTATCCTAATATTTACTAATAATCCTACTATTCTTCAGATTAGGGGTCTTACATGGTGACATTTTACTATATTTGAAAATATTTTTAACAATTTTACCATTTAAAACAATTTCTCTTATGGAAATTTTTGTTAGATCTATAAATATTATTCAATTTTTTCCTATCCGTTACAATTCTCTATAATATGGCATAGATTCTGGGGGAAGATATATAATATAGTTGTCAAAATGAATATAGTTTAATTTTTATAGTGTTTGTAATATCATCCTCGAAGTTAGAGATTTGATTCTCTATTCATATTTTTGGCGAAAGAAAAAGAAAAAAAAGGAAAGATGTATAGTATAGTTGTGTACTCCTATACAAGTGTCGGTCTTCTTAAACATGGAAGCTATTTTCTTAAAAGGAATAAAAAAGAAGATTAATTTTGAAACAAACGACAAAGTTGGTATTATTTAATTAAGATTATATAGGCAATCTTTATGTACTTGATTTTTTTAATAAAACCACAAACGAAATTATTTTAAAAAAGGCATAGGAGAATTATTGAAGTTTTCCTGTTTCGAAAATTGGAATCTTTACTTTTGTACCATTTGTCTCATGTCATCCACACGTTTTGGTTTGAATGGCTTTAATTAAAGTAGAAGCCAACGCTATTTCTTTACTGTCTTCTTTACCAACCTGCATTATATTTATTTCGAGAATTTCAAACTAATTGGAAGGCCCCTTTGTGAGTGATTTTAGGTCAAATCATTTTTTTGGAAGGTAGGTTAAATCATTTAATTATGTTTACTTCTTATAATAAATTATTTTGATATTTGGTTTCAAGTTTGCTCTTCTAATGTACTTTTTATGGCATTTTCCAGGAGGCTGGAGTCTGAAATGATTGACAAAATCACCGAAGAAATACTTAAAAAACTAGACCGTTTGACATTAAATGATGTTGATGGTCTAGTTGGAATTAAAGATCGTGTGGAGCGTATTGAGTCTTGTCTACGTGCTGATGATTCCAATGATGATGTTCGCATTTTAGGCATTTGGGGAATGAGTGACATTGTCATAAAATAAAGAATAAATTTGATGGTTACAGAACGTAAGGGAAAATTCTAGTAAAAACGGAAGTGGACTACCATATTTACAAAACAAGCTTCTAACTCAAGTTATGCAGGAGGAGAATCTTCCACTGGATATTTCACGTACATTCCTTAAATATAGGCTACGTGGAAGGAAGGTGCTCATTGTTCTTGATGATATTGATTACATGGAACAATTAGAGAGTGACAAGGATGAAAATTGGATTACTCAGTAG

mRNA sequence

ATGGGTGCTCGTGGTGAGAGCCTCATTGACAGGAGGCTGGAGTCTGAAATGATTGACAAAATCACCGAAGAAATACTTAAAAAACTAGACCGTTTGACATTAAATGATGTTGATGGTCTAGTTGGAATTAAAGATCGTGTGGAGCGTATTGAGTCTTGTCTACGTGCTGATGATTCCAATGATGATAACGTAAGGGAAAATTCTAGTAAAAACGGAAGTGGACTACCATATTTACAAAACAAGCTTCTAACTCAAGTTATGCAGGAGGAGAATCTTCCACTGGATATTTCACGTACATTCCTTAAATATAGGCTACGTGGAAGGAAGGTGCTCATTGTTCTTGATGATATTGATTACATGGAACAATTAGAGAGTGACAAGGATGAAAATTGGATTACTCAGTAG

Coding sequence (CDS)

ATGGGTGCTCGTGGTGAGAGCCTCATTGACAGGAGGCTGGAGTCTGAAATGATTGACAAAATCACCGAAGAAATACTTAAAAAACTAGACCGTTTGACATTAAATGATGTTGATGGTCTAGTTGGAATTAAAGATCGTGTGGAGCGTATTGAGTCTTGTCTACGTGCTGATGATTCCAATGATGATAACGTAAGGGAAAATTCTAGTAAAAACGGAAGTGGACTACCATATTTACAAAACAAGCTTCTAACTCAAGTTATGCAGGAGGAGAATCTTCCACTGGATATTTCACGTACATTCCTTAAATATAGGCTACGTGGAAGGAAGGTGCTCATTGTTCTTGATGATATTGATTACATGGAACAATTAGAGAGTGACAAGGATGAAAATTGGATTACTCAGTAG

Protein sequence

MGARGESLIDRRLESEMIDKITEEILKKLDRLTLNDVDGLVGIKDRVERIESCLRADDSNDDNVRENSSKNGSGLPYLQNKLLTQVMQEENLPLDISRTFLKYRLRGRKVLIVLDDIDYMEQLESDKDENWITQ

Homology

[Close]

BLAST of Bhi04G002073 vs. TAIR 10
Match: AT5G41750.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 50.1 bits (118), Expect = 1.7e-06
Identity = 45/164 (27.44%), Postives = 79/164 (48.17%), Query Frame = 0


Query: 5   GESLIDRRLESEMIDKITEEILKKLDRLTLNDVDGLVGIKDRVERIES--CLRADD---- 64
           GE  ++   E++MI KI  ++  KL+     D +G+VG++  ++R+ S  CL +D+    
Sbjct: 151 GEHSLNWDNEAKMIQKIVTDVSDKLNLTPSRDFEGMVGMEAHLKRLNSLLCLESDEVKMI 210

Query: 65  --------SNDDNVRENSSKNGSGLPY----------------------LQNKLLTQVMQ 124
                         R   +K  S  P+                      LQ +LL+++++
Sbjct: 211 GIWGPAGIGKTTIARTLFNKISSIFPFKCFMENLKGSIKGGAEHYSKLSLQKQLLSEILK 270

Query: 125 EENLPLDISRTFLKYRLRGRKVLIVLDDIDYMEQLE-SDKDENW 132
           +EN+ +    T +K  L  +KVLI+LDD+D +EQLE   +D +W
Sbjct: 271 QENMKIHHLGT-IKQWLHDQKVLIILDDVDDLEQLEVLAEDPSW 313

[Close]

BLAST of Bhi04G002073 vs. TAIR 10
Match: AT5G41750.2 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 50.1 bits (118), Expect = 1.7e-06
Identity = 45/164 (27.44%), Postives = 79/164 (48.17%), Query Frame = 0


Query: 5   GESLIDRRLESEMIDKITEEILKKLDRLTLNDVDGLVGIKDRVERIES--CLRADD---- 64
           GE  ++   E++MI KI  ++  KL+     D +G+VG++  ++R+ S  CL +D+    
Sbjct: 151 GEHSLNWDNEAKMIQKIVTDVSDKLNLTPSRDFEGMVGMEAHLKRLNSLLCLESDEVKMI 210

Query: 65  --------SNDDNVRENSSKNGSGLPY----------------------LQNKLLTQVMQ 124
                         R   +K  S  P+                      LQ +LL+++++
Sbjct: 211 GIWGPAGIGKTTIARTLFNKISSIFPFKCFMENLKGSIKGGAEHYSKLSLQKQLLSEILK 270

Query: 125 EENLPLDISRTFLKYRLRGRKVLIVLDDIDYMEQLE-SDKDENW 132
           +EN+ +    T +K  L  +KVLI+LDD+D +EQLE   +D +W
Sbjct: 271 QENMKIHHLGT-IKQWLHDQKVLIILDDVDDLEQLEVLAEDPSW 313

[Close]

BLAST of Bhi04G002073 vs. TAIR 10
Match: AT5G40910.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 46.6 bits (109), Expect = 1.8e-05
Identity = 42/159 (26.42%), Postives = 75/159 (47.17%), Query Frame = 0


Query: 5   GESLIDRRLESEMIDKITEEILKKLDRLTLNDVDGLVGIKDRVERIES--CLRADD---- 64
           GE  +    E+E+I KI  ++  KL+     D +G+VG++  + +++S  CL +DD    
Sbjct: 150 GEHSLSWANEAELIQKIATDVSNKLNLTPSRDFEGMVGLEAHLTKLDSFLCLESDDVKMI 209

Query: 65  ----------SNDDNVRENSSKNGSGLP---------------YLQNKLLTQVMQEENLP 124
                     +       N    G  L                 LQNKLL++++ ++++ 
Sbjct: 210 GIWGPAGIGKTTIARALFNQLSTGFRLSCFMGTIDVNDYDSKLCLQNKLLSKILNQKDMK 269

Query: 125 LDISRTFLKYRLRGRKVLIVLDDIDYMEQLE-SDKDENW 132
           +       ++ L  ++VLIVLDD+D +EQLE   K+ +W
Sbjct: 270 IHHLGAIEEW-LHNQRVLIVLDDVDDLEQLEVLAKESSW 307

[Close]

BLAST of Bhi04G002073 vs. TAIR 10
Match: AT1G66090.1 (Disease resistance protein (TIR-NBS class) )

HSP 1 Score: 46.2 bits (108), Expect = 2.4e-05
Identity = 47/170 (27.65%), Postives = 73/170 (42.94%), Query Frame = 0


Query: 5   GESLIDRRLESEMIDKITEEILKKLDRLTLNDVDGLVGIKDRVERIESCLRAD------- 64
           G  + DR  E+ MI+KI  ++ +KL+     D D +VGIK  + +IES L  D       
Sbjct: 164 GVHVQDRDDEANMIEKIATDVSEKLNATESKDFDEMVGIKAHLTKIESLLSLDYDKVKIV 223

Query: 65  ------------------------------------DSNDDNVRENSSKNGSGLPYLQNK 124
                                                SN  +  E SSK       LQ +
Sbjct: 224 GISGPAGIGKSTIARALHNLLSSSFHLSCFMENLISQSNPHSSLEYSSKLS-----LQEQ 283

Query: 125 LLTQVMQEENLPLDISRTFLKYRLRGRKVLIVLDDIDYMEQLESDKDENW 132
           LL+QV+ E+++ +      ++ RL  ++VLI+LDD+  +EQLE   +  W
Sbjct: 284 LLSQVLNEKDIRIR-HLGAIQERLHDQRVLIILDDVTSLEQLEVLANIKW 327

[Close]

BLAST of Bhi04G002073 vs. TAIR 10
Match: AT4G12010.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 45.8 bits (107), Expect = 3.1e-05
Identity = 38/148 (25.68%), Postives = 70/148 (47.30%), Query Frame = 0


Query: 14  ESEMIDKITEEILKKLDRLTLNDVDGLVGIKDRVERIESCLRADDSND------------ 73
           E++++D+I  +  KKL+ L  +  +GLVGI+ R++ +E  L  +D +             
Sbjct: 161 EAKLVDEIAVDTFKKLNDLAPSGNEGLVGIESRLKNLEKLLSWEDLDTVHIIGIVGMVGI 220

Query: 74  -----------------------DNVRENSSKNGSGLPYLQNKLLTQVMQEENLPLDI-- 124
                                   N+RENS +  SGL  L  KL + V+ + +L +    
Sbjct: 221 GKTTLADCLYGRMRGQFDGSCFLTNIRENSGR--SGLESLLQKLFSTVLNDRDLEIGAPG 280

[Close]

BLAST of Bhi04G002073 vs. ExPASy Swiss-Prot
Match: Q9SZ66 (Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 4.4e-04
Identity = 38/148 (25.68%), Postives = 70/148 (47.30%), Query Frame = 0


Query: 14  ESEMIDKITEEILKKLDRLTLNDVDGLVGIKDRVERIESCLRADDSND------------ 73
           E++++D+I  +  KKL+ L  +  +GLVGI+ R++ +E  L  +D +             
Sbjct: 161 EAKLVDEIAVDTFKKLNDLAPSGNEGLVGIESRLKNLEKLLSWEDLDTVHIIGIVGMVGI 220

Query: 74  -----------------------DNVRENSSKNGSGLPYLQNKLLTQVMQEENLPLDI-- 124
                                   N+RENS +  SGL  L  KL + V+ + +L +    
Sbjct: 221 GKTTLADCLYGRMRGQFDGSCFLTNIRENSGR--SGLESLLQKLFSTVLNDRDLEIGAPG 280

[Close]

BLAST of Bhi04G002073 vs. ExPASy TrEMBL
Match: A0A6J1DE10 (TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111020196 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 4.1e-13
Identity = 65/148 (43.92%), Postives = 85/148 (57.43%), Query Frame = 0


Query: 14  ESEMIDKITEEILKKLDRLTLNDV-DGLVGIKDRVERIESCLRADDSND----------- 73
           E+  I++I+ EILKKL+RLT  DV +GLVGI+ R+ERIES +R    +            
Sbjct: 29  EATFIEEISGEILKKLNRLTPIDVHEGLVGIEARIERIESRVRVGSRDVCILGIWGMGGI 88

Query: 74  -----------------------DNVRENSSKNGSGLPYLQNKLLTQVMQE--ENLPLDI 125
                                   NVREN  K+  GL +LQ KLL +V++E  + L  +I
Sbjct: 89  GKTTLARALFDRIKSQFDGCCFLKNVRENQIKH--GLSHLQEKLLCEVLEERRDQLIGNI 148

[Close]

BLAST of Bhi04G002073 vs. ExPASy TrEMBL
Match: A0A6P5SJ86 (TMV resistance protein N-like OS=Prunus avium OX=42229 GN=LOC110758527 PE=4 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 6.1e-09
Identity = 51/151 (33.77%), Postives = 78/151 (51.66%), Query Frame = 0


Query: 13  LESEMIDKITEEILKKLDRLTLNDVDGLVGIKDRVERIESCLRADDSNDD---------- 72
           LES+++ K+ +++L KL+R T +D+ GLVGI++R+E IES L  DD + D          
Sbjct: 154 LESDLVKKVVKDVLSKLNRKTSSDLKGLVGIENRIEEIESLLCIDDDSLDAYSVGIWGMG 213

Query: 73  --------------------------NVRENSSKNGSGLPYLQNKLLTQVMQEENLPLD- 125
                                     NVR  S +   GL +L+N LL +++ +ENL +D 
Sbjct: 214 GIGKTTLADATFHRISSKFEAACFLANVRVKSEEK-DGLIHLRNTLLQKILDDENLNIDT 273

[Close]

BLAST of Bhi04G002073 vs. ExPASy TrEMBL
Match: A0A077D491 (RGA8 (Fragment) OS=Lagenaria siceraria OX=3668 GN=RGA8 PE=4 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 8.0e-09
Identity = 38/57 (66.67%), Postives = 45/57 (78.95%), Query Frame = 0


Query: 62  DNVRENSSKNGSGLPYLQNKLLTQVMQEE-NLPLDISRTFLKYRLRGRKVLIVLDDI 118
           +NVRENS KNG GLP L+  LL+QV QEE +L + ISR FLK RL G+KVLIVLDD+
Sbjct: 29  ENVRENSGKNGHGLPNLRKMLLSQVTQEEQHLSMHISRAFLKNRLSGKKVLIVLDDV 85

[Close]

BLAST of Bhi04G002073 vs. ExPASy TrEMBL
Match: A0A077D6S8 (RGA9 (Fragment) OS=Lagenaria siceraria OX=3668 GN=RGA9 PE=4 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 8.0e-09
Identity = 38/57 (66.67%), Postives = 45/57 (78.95%), Query Frame = 0


Query: 62  DNVRENSSKNGSGLPYLQNKLLTQVMQEE-NLPLDISRTFLKYRLRGRKVLIVLDDI 118
           +NVRENS KNG GLP L+  LL+QV QEE +L + ISR FLK RL G+KVLIVLDD+
Sbjct: 30  ENVRENSGKNGHGLPNLRKMLLSQVTQEEQHLSMHISRAFLKNRLSGKKVLIVLDDV 86

[Close]

BLAST of Bhi04G002073 vs. ExPASy TrEMBL
Match: A0A5E4FYA8 (PREDICTED: TMV resistance OS=Prunus dulcis OX=3755 GN=ALMOND_2B015345 PE=4 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 8.0e-09
Identity = 53/149 (35.57%), Postives = 78/149 (52.35%), Query Frame = 0


Query: 12  RLESEMIDKITEEILKKLDRLTLNDVDGLVGIKDRVERIESCLRADDSND---------- 71
           R E+ +++ + E+IL KL+R + +D+ GLV I+ ++E+IES L  D              
Sbjct: 137 RTEAHLVETLVEDILTKLNRKSSSDLKGLVAIESQIEQIESSLCIDSPEVCFVGIWGIGI 196

Query: 72  -----------------------DNVRENSSKNGSGLPYLQNKLLTQVMQEENLPLD--- 125
                                   NVRE S K+  GL +L+NKLL  ++++ENL +D   
Sbjct: 197 GKTTLAGAVYSRLSSKFKTSCFLANVREESEKH--GLNHLRNKLLRVLLEDENLTIDTPS 256

The following BLAST results are available for this feature:

BLAST of Bhi04G002073 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G41750.1	1.7e-06	27.44	Disease resistance protein (TIR-NBS-LRR class) family	[more]
AT5G41750.2	1.7e-06	27.44	Disease resistance protein (TIR-NBS-LRR class) family	[more]
AT5G40910.1	1.8e-05	26.42	Disease resistance protein (TIR-NBS-LRR class) family	[more]
AT1G66090.1	2.4e-05	27.65	Disease resistance protein (TIR-NBS class)	[more]
AT4G12010.1	3.1e-05	25.68	Disease resistance protein (TIR-NBS-LRR class) family	[more]

BLAST of Bhi04G002073 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SZ66	4.4e-04	25.68	Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=...	[more]

BLAST of Bhi04G002073 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DE10	4.1e-13	43.92	TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111020196 PE=...	[more]
A0A6P5SJ86	6.1e-09	33.77	TMV resistance protein N-like OS=Prunus avium OX=42229 GN=LOC110758527 PE=4 SV=1	[more]
A0A077D491	8.0e-09	66.67	RGA8 (Fragment) OS=Lagenaria siceraria OX=3668 GN=RGA8 PE=4 SV=1	[more]
A0A077D6S8	8.0e-09	66.67	RGA9 (Fragment) OS=Lagenaria siceraria OX=3668 GN=RGA9 PE=4 SV=1	[more]
A0A5E4FYA8	8.0e-09	35.57	PREDICTED: TMV resistance OS=Prunus dulcis OX=3755 GN=ALMOND_2B015345 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 57..76
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 36..124

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi04M002073	Bhi04M002073	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
molecular_function	GO:0043531	ADP binding