Bhi04G001992 (gene) Wax gourd (B227) v1

Overview
NameBhi04G001992
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionprotein NUCLEAR FUSION DEFECTIVE 4-like
Locationchr4: 66446267 .. 66449992 (-)
RNA-Seq ExpressionBhi04G001992
SyntenyBhi04G001992
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGAGAGTCCAAGAAAAGGAAAAAAGGAAAGAAAAAAAACGTTACTCAAAAACTCAATGATTATATTTCAAAAGGATAAAACGTTTGTAGTTGGATCACCATTCCCAATTGAAATAATCAAATGGAAGGTAAAATTTCTGATAAATAATCAAATTTGCTTCGTTGTCGTCTTCTAATTTGAATCCGCCACAGCCACAGGCCACAGCCACGGCCATAGACATATCCAACTCCTTGTTTCTTGTATATCGTGCATAACAGAACACAGTGATTGCCAACGCCTTGATGGAGATTTTTCTAATTAATTATCGAAAACATCCATTTTTATTTTGCCACCCCATTTCCATCGATCGACCCCACATTATCATTCCCATTTCTCATCGCCTGTGCTGTGTTCTGCCCCACCCATCTCTGCCAATGCCCTTTTAGAGACCTCCCCATTTCATTTTGATTCTCTAATTTTTCCTTTACCACAGAGATTTGGGTTTCTGGGCATTGATGGGTCGGTGGAATGAGAAACTTGTGGCCTTTTTCAACAATAGATGGTTGGTTTTTGTAGCTGCAATATGGCTTCAATCATGCGCTGGAATTGGGTATCTTTTTGGAAGTATATCGCCGGTTATCAAAACCAATTTGAGTTACAATCAGAGGCAAATTGCGCGTCTCGGTGTTGCTAAAGACCTTGGCGATAGCGTCGGCATTTTGGCCGCTACCTTGTCGGAGATTTTGCCCTTTTGGGGATCTCTCTTGGTCGGTGCTATCCATAACTTTGTTGGGTATGGTTGGATTTGGCTCATCGTCACTGGTCGAGCTCCCGTTTTGCCTCTCTGGGCTGTAAGTTTTCATTCTCCTGTTCTTTCCCTTTAATGCCCCTTGTTTGAGTTTCTATTCGGATTCCATTTCATTTTCCTTATGGGTATGGATCGTTGTCTTTAGATGTTTCCATCGGGATATCCATATTCAGATCTTGATTTGATGGTTTTAATGGAGGTGTTTTGCTTTTATGAACTAATTTCAAAAGTTGCATAGTGGTCAACGAGTTGGAATCTGTTTTAGTATCAATATGATGATTGTTAGATTAAAGATAGTTTGGTATTTTCCTTGGAATTTCTCTGAGAGATTACTCTAAGTTGTGTTGTAGTTAAATGCAGCAGGAAGCAGATTATCTGTTTGATGATTTGTTTCTGTAGGATTGTGGAAGCAATTTTATTGAATATTTCATGGATGCATAACTGATTGCTGGGAATAGAACTTTACAAACCGATTTGCTGAATATATAGGCGGATTGAAACTATTGAAAAGTCCTTTTTGGAAGGTCCAGAATCAATCCTTCCTAACCAGTTCGTTAAGATTGGTAAAATGCATCATCTTCTGGCCATTTAACTCTAAAATTCTTAGTTTGAAGAAAACAATGGCTATATGGAAGAAAATGAGGGGTGAGATGCAGGAATGGCTGTAACTGAACCACTCTAACCAAATAAAGTTTGAAGAGCAAATTGTTAAGCCATTTTTTTTTTCTTTTCATCATTTTTGTTTAGTCACGTATGGATATCATTGTGCAGATGTGCATTCTTGTATTTCTGGGAACGAATGGGGAGACGTACTTCAATACGGTTGCTCTGGTTTCTTGTGTACAAAACTTTCCGAAAAGCAGGGGCCCTGTCGTCGGGATTCTGAAAGGCTTTGCTGGTTTAAGTGGTGCAATTTTGACTCAGATATATGCAATCGTGCATGCTCCTGATTCAGCCAATCTTATATTCATGGTTGCAGTAGGTCCTGCACTGGTAGCTATTGGTGTGATGTTTTTTATCAGACCTGTCGCTGGTCATAGACAAGTAAGGCCGTCCGATAGCATGAGCTTTACTAGTGTTTATGGTGTCTGTCTCCTTTTGGCTGCTTATTTGATGGGAGTCATGCTTGTTGAGGATCTTGTTACCTTGAACCCCACCGTGATAGCCATTTTTACTGGGGTTATGTTTGTCATCCTTCTTACACCCTTTTTGATTCCCGTGACATTGACTTTTAGCTCGGAGACAATGACATATGCTGAGCAAGAGGCTCTCCTACCACCTTCAGAGAAACAGGAACCTGCTAGATCTGAACCAGATGGTAATGAAGTGATATTTAGTGAGGTGGAAGATGAGAAATCAGAGGGAGAGGACTTACTTCCTGCATTAGAGAGACAAAAACGCATTGCTCAGCTGCAAGCAAGACTATTGCAAGCAGCTGCAGAAGGAGCAGTAAGAGTGAAGAGGAGGAAGGGTCCGCGTCGAGGAGAAGACTTCACTTTGGGTCAAGCTTTAATCAAAGCCGATTTTTGGCTTATATTCGTCTCCCTTCTTCTTGGTTCTGGAACTGGATTGACTGTGATCGATAACCTCGGACAGATGAGCGAGTCTTTAGGTTATGATAATACACATATTTTTGTATCCCTGATCAGCATATGGAACTTTCTCGGCCGTGTTGGTGGTGGATACCTCTCTGAGATTGTTGTGAGGTACAAAAGTTATTTTAGTTGTCTCAAGGCAAAGTAAATAAAACTTTTTACTTCTTAGAATGTTCCATTATATCTTACTCTCATTTTCTTCAACAGAGATTTTGCTTATCCAAGACCCATTGCAATGGCGATTGCACAATTCCTTATGATATTCGGACATCTCTTCATCGGCATGGGATGGCCTGGGGCAATGTACATTGGTACTCTCATAACGGGGCTCGGGTATGGGGCTCACTGGGCAATTGTGCCTGCTACTGCCTCTGAATTGTTTGGCTTGAAGAAGTTTGGGGCTTTATACAACTTCCTCACTCTCTCAAATCCTATGGGATCTCTGATATTTTCGAGCTTAATCGCGAGCGGTATATATGACAGTGAAGCAGAGAAGCAAGCTCATAACCATCCCCCACATCTGCAGAGCTCCTCATCATTTTTGTTGAGTAGACTTTACGCCGATGGACCGCACGAATGTGAGGGTGCCATATGTTTCTTTTTAACTTGTATGTTAATGGCTGGATTATGTGCGATTGCAGGCATGTTAAGTTTAATTTTGGTGTATCGGACAAAGGGTGTTTACTCCAACCTCTATGGGAAATCTCGTACATCGACGCTTTCGTAGAATGGTTTCAACTTTCATCAATCATGCTACCTTTTCCATGTTCGTTTTTTCGAGATCGAGTGAAATTTTTGCTGAGATGGCTTTGCAAGAACTCACAAGAAGCAGCCATGGACTCGAAATGGACTTGCTTCAGTCTTACAGTTCAATTCACCCTTTTGGTATAAAAAGCCTATTCCTGTGTGTTGGAGACTTCAGTTGGTGGAAGAAAAACATGCACCTTCGTTTCCTATCTTCCACCCAAAATAGAAAATAAAGAAAAAGAAATGTATGAAATATAATTGTTGTGAAACATTTGATATTTAGGATTCATCATTATTGATCAGTGAATTAAAAAAAAGTATGCTATTTATTTGTTTAATTTGCATTTTTTGTTGTCTTCTCTCTTTAGATTGTCATGTATAGGCTATAATTGTGACTTTTTCAACACCTATCATTTCACTGTCTGTGGACATTTCTTTTTCAATATTTATTGGAGAACCACATATTTTTATTATTGTTGTTGTTGTAATATATTTTAATAGAATTTCTAATAACATAATAGGTTTGATAATGTTTAGAAAAA

mRNA sequence

CGAGAGTCCAAGAAAAGGAAAAAAGGAAAGAAAAAAAACGTTACTCAAAAACTCAATGATTATATTTCAAAAGGATAAAACGTTTGTAGTTGGATCACCATTCCCAATTGAAATAATCAAATGGAAGAGATTTGGGTTTCTGGGCATTGATGGGTCGGTGGAATGAGAAACTTGTGGCCTTTTTCAACAATAGATGGTTGGTTTTTGTAGCTGCAATATGGCTTCAATCATGCGCTGGAATTGGGTATCTTTTTGGAAGTATATCGCCGGTTATCAAAACCAATTTGAGTTACAATCAGAGGCAAATTGCGCGTCTCGGTGTTGCTAAAGACCTTGGCGATAGCGTCGGCATTTTGGCCGCTACCTTGTCGGAGATTTTGCCCTTTTGGGGATCTCTCTTGGTCGGTGCTATCCATAACTTTGTTGGGTATGGTTGGATTTGGCTCATCGTCACTGGTCGAGCTCCCGTTTTGCCTCTCTGGGCTATGTGCATTCTTGTATTTCTGGGAACGAATGGGGAGACGTACTTCAATACGGTTGCTCTGGTTTCTTGTGTACAAAACTTTCCGAAAAGCAGGGGCCCTGTCGTCGGGATTCTGAAAGGCTTTGCTGGTTTAAGTGGTGCAATTTTGACTCAGATATATGCAATCGTGCATGCTCCTGATTCAGCCAATCTTATATTCATGGTTGCAGTAGGTCCTGCACTGGTAGCTATTGGTGTGATGTTTTTTATCAGACCTGTCGCTGGTCATAGACAAGTAAGGCCGTCCGATAGCATGAGCTTTACTAGTGTTTATGGTGTCTGTCTCCTTTTGGCTGCTTATTTGATGGGAGTCATGCTTGTTGAGGATCTTGTTACCTTGAACCCCACCGTGATAGCCATTTTTACTGGGGTTATGTTTGTCATCCTTCTTACACCCTTTTTGATTCCCGTGACATTGACTTTTAGCTCGGAGACAATGACATATGCTGAGCAAGAGGCTCTCCTACCACCTTCAGAGAAACAGGAACCTGCTAGATCTGAACCAGATGGTAATGAAGTGATATTTAGTGAGGTGGAAGATGAGAAATCAGAGGGAGAGGACTTACTTCCTGCATTAGAGAGACAAAAACGCATTGCTCAGCTGCAAGCAAGACTATTGCAAGCAGCTGCAGAAGGAGCAGTAAGAGTGAAGAGGAGGAAGGGTCCGCGTCGAGGAGAAGACTTCACTTTGGGTCAAGCTTTAATCAAAGCCGATTTTTGGCTTATATTCGTCTCCCTTCTTCTTGGTTCTGGAACTGGATTGACTGTGATCGATAACCTCGGACAGATGAGCGAGTCTTTAGGTTATGATAATACACATATTTTTGTATCCCTGATCAGCATATGGAACTTTCTCGGCCGTGTTGGTGGTGGATACCTCTCTGAGATTGTTGTGAGAGATTTTGCTTATCCAAGACCCATTGCAATGGCGATTGCACAATTCCTTATGATATTCGGACATCTCTTCATCGGCATGGGATGGCCTGGGGCAATGTACATTGGTACTCTCATAACGGGGCTCGGGTATGGGGCTCACTGGGCAATTGTGCCTGCTACTGCCTCTGAATTGTTTGGCTTGAAGAAGTTTGGGGCTTTATACAACTTCCTCACTCTCTCAAATCCTATGGGATCTCTGATATTTTCGAGCTTAATCGCGAGCGGTATATATGACAGTGAAGCAGAGAAGCAAGCTCATAACCATCCCCCACATCTGCAGAGCTCCTCATCATTTTTGTTGAGTAGACTTTACGCCGATGGACCGCACGAATGTGAGGGTGCCATATGTTTCTTTTTAACTTGTATGTTAATGGCTGGATTATGTGCGATTGCAGGCATGTTAAGTTTAATTTTGGTGTATCGGACAAAGGGTGTTTACTCCAACCTCTATGGGAAATCTCGTACATCGACGCTTTCGTAGAATGGTTTCAACTTTCATCAATCATGCTACCTTTTCCATGTTCGTTTTTTCGAGATCGAGTGAAATTTTTGCTGAGATGGCTTTGCAAGAACTCACAAGAAGCAGCCATGGACTCGAAATGGACTTGCTTCAGTCTTACAGTTCAATTCACCCTTTTGGTATAAAAAGCCTATTCCTGTGTGTTGGAGACTTCAGTTGGTGGAAGAAAAACATGCACCTTCGTTTCCTATCTTCCACCCAAAATAGAAAATAAAGAAAAAGAAATGTATGAAATATAATTGTTGTGAAACATTTGATATTTAGGATTCATCATTATTGATCAGTGAATTAAAAAAAAGTATGCTATTTATTTGTTTAATTTGCATTTTTTGTTGTCTTCTCTCTTTAGATTGTCATGTATAGGCTATAATTGTGACTTTTTCAACACCTATCATTTCACTGTCTGTGGACATTTCTTTTTCAATATTTATTGGAGAACCACATATTTTTATTATTGTTGTTGTTGTAATATATTTTAATAGAATTTCTAATAACATAATAGGTTTGATAATGTTTAGAAAAA

Coding sequence (CDS)

ATGGGTCGGTGGAATGAGAAACTTGTGGCCTTTTTCAACAATAGATGGTTGGTTTTTGTAGCTGCAATATGGCTTCAATCATGCGCTGGAATTGGGTATCTTTTTGGAAGTATATCGCCGGTTATCAAAACCAATTTGAGTTACAATCAGAGGCAAATTGCGCGTCTCGGTGTTGCTAAAGACCTTGGCGATAGCGTCGGCATTTTGGCCGCTACCTTGTCGGAGATTTTGCCCTTTTGGGGATCTCTCTTGGTCGGTGCTATCCATAACTTTGTTGGGTATGGTTGGATTTGGCTCATCGTCACTGGTCGAGCTCCCGTTTTGCCTCTCTGGGCTATGTGCATTCTTGTATTTCTGGGAACGAATGGGGAGACGTACTTCAATACGGTTGCTCTGGTTTCTTGTGTACAAAACTTTCCGAAAAGCAGGGGCCCTGTCGTCGGGATTCTGAAAGGCTTTGCTGGTTTAAGTGGTGCAATTTTGACTCAGATATATGCAATCGTGCATGCTCCTGATTCAGCCAATCTTATATTCATGGTTGCAGTAGGTCCTGCACTGGTAGCTATTGGTGTGATGTTTTTTATCAGACCTGTCGCTGGTCATAGACAAGTAAGGCCGTCCGATAGCATGAGCTTTACTAGTGTTTATGGTGTCTGTCTCCTTTTGGCTGCTTATTTGATGGGAGTCATGCTTGTTGAGGATCTTGTTACCTTGAACCCCACCGTGATAGCCATTTTTACTGGGGTTATGTTTGTCATCCTTCTTACACCCTTTTTGATTCCCGTGACATTGACTTTTAGCTCGGAGACAATGACATATGCTGAGCAAGAGGCTCTCCTACCACCTTCAGAGAAACAGGAACCTGCTAGATCTGAACCAGATGGTAATGAAGTGATATTTAGTGAGGTGGAAGATGAGAAATCAGAGGGAGAGGACTTACTTCCTGCATTAGAGAGACAAAAACGCATTGCTCAGCTGCAAGCAAGACTATTGCAAGCAGCTGCAGAAGGAGCAGTAAGAGTGAAGAGGAGGAAGGGTCCGCGTCGAGGAGAAGACTTCACTTTGGGTCAAGCTTTAATCAAAGCCGATTTTTGGCTTATATTCGTCTCCCTTCTTCTTGGTTCTGGAACTGGATTGACTGTGATCGATAACCTCGGACAGATGAGCGAGTCTTTAGGTTATGATAATACACATATTTTTGTATCCCTGATCAGCATATGGAACTTTCTCGGCCGTGTTGGTGGTGGATACCTCTCTGAGATTGTTGTGAGAGATTTTGCTTATCCAAGACCCATTGCAATGGCGATTGCACAATTCCTTATGATATTCGGACATCTCTTCATCGGCATGGGATGGCCTGGGGCAATGTACATTGGTACTCTCATAACGGGGCTCGGGTATGGGGCTCACTGGGCAATTGTGCCTGCTACTGCCTCTGAATTGTTTGGCTTGAAGAAGTTTGGGGCTTTATACAACTTCCTCACTCTCTCAAATCCTATGGGATCTCTGATATTTTCGAGCTTAATCGCGAGCGGTATATATGACAGTGAAGCAGAGAAGCAAGCTCATAACCATCCCCCACATCTGCAGAGCTCCTCATCATTTTTGTTGAGTAGACTTTACGCCGATGGACCGCACGAATGTGAGGGTGCCATATGTTTCTTTTTAACTTGTATGTTAATGGCTGGATTATGTGCGATTGCAGGCATGTTAAGTTTAATTTTGGTGTATCGGACAAAGGGTGTTTACTCCAACCTCTATGGGAAATCTCGTACATCGACGCTTTCGTAG

Protein sequence

MGRWNEKLVAFFNNRWLVFVAAIWLQSCAGIGYLFGSISPVIKTNLSYNQRQIARLGVAKDLGDSVGILAATLSEILPFWGSLLVGAIHNFVGYGWIWLIVTGRAPVLPLWAMCILVFLGTNGETYFNTVALVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIVHAPDSANLIFMVAVGPALVAIGVMFFIRPVAGHRQVRPSDSMSFTSVYGVCLLLAAYLMGVMLVEDLVTLNPTVIAIFTGVMFVILLTPFLIPVTLTFSSETMTYAEQEALLPPSEKQEPARSEPDGNEVIFSEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALIKADFWLIFVSLLLGSGTGLTVIDNLGQMSESLGYDNTHIFVSLISIWNFLGRVGGGYLSEIVVRDFAYPRPIAMAIAQFLMIFGHLFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASELFGLKKFGALYNFLTLSNPMGSLIFSSLIASGIYDSEAEKQAHNHPPHLQSSSSFLLSRLYADGPHECEGAICFFLTCMLMAGLCAIAGMLSLILVYRTKGVYSNLYGKSRTSTLS
Homology
BLAST of Bhi04G001992 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 808.5 bits (2087), Expect = 3.5e-234
Identity = 408/589 (69.27%), Postives = 485/589 (82.34%), Query Frame = 0

Query: 6   EKLVAFFNNRWLVFVAAIWLQSCAGIGYLFGSISPVIKTNLSYNQRQIARLGVAKDLGDS 65
           EK V+F NNRWLVFVAA+W+QSCAGIGYLFGSISPVIK++L+YNQ++++RLGVAKDLGDS
Sbjct: 7   EKFVSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDS 66

Query: 66  VGILAATLSEILPFWGSLLVGAIHNFVGYGWIWLIVTGRAPVLPLWAMCILVFLGTNGET 125
           VG +A TLSEILP W +LLVGA+ N +GYGW+WLIVTGRAP+LPLWAMC+L+F+G NGET
Sbjct: 67  VGFIAGTLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGET 126

Query: 126 YFNTVALVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIVHAPDSANLIFMVAVGPA 185
           YFNT ALVS VQNFPKSRGPVVGILKGFAGL GAI++QIY ++H+ + A+LI MVAV PA
Sbjct: 127 YFNTGALVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPA 186

Query: 186 LVAIGVMFFIRPVAGHRQVRPSDSMSFTSVYGVCLLLAAYLMGVMLVEDLVTLNPTVIAI 245
           +V + +MFFIRPV GH+Q+RP+D  SFT +YGVCLLLAAYLM VML++DLV ++  VI +
Sbjct: 187 VVVVCLMFFIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITV 246

Query: 246 FTGVMFVILLTPFLIPVTLTFSSETMTYAE--QEALLPPSEKQEPARSEPDGNEVIFSEV 305
           FT V+FVIL+ P L+P+  +F +ET    +  +E L+P  E QEP    PD   +I SEV
Sbjct: 247 FTIVLFVILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPD---LILSEV 306

Query: 306 EDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALIKAD 365
           EDEK +  DLLPA ER KRIA LQA+L+QAAAEGAVRV RR+GP RGEDFTL QAL+KAD
Sbjct: 307 EDEKPKDVDLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKAD 366

Query: 366 FWLIFVSLLLGSGTGLTVIDNLGQMSESLGYDNTHIFVSLISIWNFLGRVGGGYLSEIVV 425
           FWLIF SLLLGSG+GLTVIDNLGQMS+SLGYDNTH+ VS+ISIWNFLGR+GGGY SE+VV
Sbjct: 367 FWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHVLVSMISIWNFLGRIGGGYFSELVV 426

Query: 426 RDFAYPRPIAMAIAQFLMIFGHLFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASELFG 485
           RD+AYPRP+AMA+AQ +M  GH+F   GWPGAMYIGTL+ GLGYGAHWAIVPATASELFG
Sbjct: 427 RDYAYPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFG 486

Query: 486 LKKFGALYNFLTLSNPMGSLIFSSLIASGIYDSEAEKQAHNHPPHLQSSSSFLLSRLYAD 545
           LKKFGALYNFLTL+NP GSL+FS +IAS IYD EAE+QAH              S    D
Sbjct: 487 LKKFGALYNFLTLANPAGSLVFSGMIASSIYDREAERQAHG-------------SVFDPD 546

Query: 546 GPHECEGAICFFLTCMLMAGLCAIAGMLSLILVYRTKGVYSNLYGKSRT 593
               C G+ICFFLT ++M+G C IA MLS+ILV RTK VY++LYGK+RT
Sbjct: 547 DALRCNGSICFFLTSLIMSGFCIIACMLSMILVRRTKSVYTHLYGKTRT 579

BLAST of Bhi04G001992 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 799.7 bits (2064), Expect = 1.6e-231
Identity = 409/590 (69.32%), Postives = 486/590 (82.37%), Query Frame = 0

Query: 6   EKLVAFFNNRWLVFVAAIWLQSCAGIGYLFGSISPVIKTNLSYNQRQIARLGVAKDLGDS 65
           E++ +F NNRWLVFVAA+W+QSCAGIGYLFGSISPVIK++L+YNQ+Q++RLGVAKDLGDS
Sbjct: 7   ERVKSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDS 66

Query: 66  VGILAATLSEILPFWGSLLVGAIHNFVGYGWIWLIVTGRAPVLPLWAMCILVFLGTNGET 125
           VG LA TLSEILP W +LLVG++ N VGYGW+WLIVTGRAP+LPLWAMCIL+F+G NGET
Sbjct: 67  VGFLAGTLSEILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGET 126

Query: 126 YFNTVALVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIVHAPDSANLIFMVAVGPA 185
           YFNT ALVS VQNFPKSRGPVVGILKGFAGL GAIL+Q+Y ++H+ D A+LIFMVAV P+
Sbjct: 127 YFNTAALVSGVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSDRASLIFMVAVAPS 186

Query: 186 LVAIGVMFFIRPVAGHRQVRPSDSMSFTSVYGVCLLLAAYLMGVMLVEDLVTLNPTVIAI 245
           +V + +MFFIRPV GHRQ+R SD+ SFT +Y VC+LLAAYLM VMLVED + L+ ++I  
Sbjct: 187 VVVVPLMFFIRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLVEDFIDLSHSIIIA 246

Query: 246 FTGVMFVILLTPFLIPV-TLTFSSET-MTYAEQEALLPPSEKQEPARS-EPD-GNEVIFS 305
           FT V+F ILL P  IP+ T  F++ T      +E LL   + Q+P +S  PD G E+IFS
Sbjct: 247 FTVVLFAILLVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELIFS 306

Query: 306 EVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALIK 365
           EVEDEK +  DLLPA+ER KRIAQLQA+L+QAAAEGAVRVKRR+GP RGEDFTL QAL+K
Sbjct: 307 EVEDEKPKEVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVK 366

Query: 366 ADFWLIFVSLLLGSGTGLTVIDNLGQMSESLGYDNTHIFVSLISIWNFLGRVGGGYLSEI 425
           ADFWLIF SLLLGSG+GLTVIDNLGQMS+SLGYDNTH+FVS+ISIWNFLGR+GGGY SE+
Sbjct: 367 ADFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHVFVSMISIWNFLGRIGGGYFSEL 426

Query: 426 VVRDFAYPRPIAMAIAQFLMIFGHLFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASEL 485
           +VRD+AYPRP+A+A+AQ +M  GH+F   GWPGAM+IGTL+ GLGYGAHWAIVPATASEL
Sbjct: 427 IVRDYAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIVPATASEL 486

Query: 486 FGLKKFGALYNFLTLSNPMGSLIFSSLIASGIYDSEAEKQAHNHPPHLQSSSSFLLSRLY 545
           FGLKKFGALYNFLTL+NP GSL+FS LIAS IYD EAE+QA               S   
Sbjct: 487 FGLKKFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQG-------------SLFN 546

Query: 546 ADGPHECEGAICFFLTCMLMAGLCAIAGMLSLILVYRTKGVYSNLYGKSR 592
            D    C G+IC+FLT ++M+G C IA  LS+ILV RTK VY+NLYGK+R
Sbjct: 547 PDDVLRCRGSICYFLTSLIMSGFCLIAAALSMILVQRTKPVYTNLYGKTR 583

BLAST of Bhi04G001992 vs. TAIR 10
Match: AT3G01930.1 (Major facilitator superfamily protein )

HSP 1 Score: 617.8 bits (1592), Expect = 8.8e-177
Identity = 326/483 (67.49%), Postives = 387/483 (80.12%), Query Frame = 0

Query: 113 MCILVFLGTNGETYFNTVALVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIVHAPD 172
           MCIL+F+G NGETYFNT ALVS VQNFPKSRGPVVGILKGFAGL GAIL+Q+Y ++H+ D
Sbjct: 1   MCILIFVGNNGETYFNTAALVSGVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSD 60

Query: 173 SANLIFMVAVGPALVAIGVMFFIRPVAGHRQVRPSDSMSFTSVYGVCLLLAAYLMGVMLV 232
            A+LIFMVAV P++V + +MFFIRPV GHRQ+R SD+ SFT +Y VC+LLAAYLM VMLV
Sbjct: 61  RASLIFMVAVAPSVVVVPLMFFIRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLV 120

Query: 233 EDLVTLNPTVIAIFTGVMFVILLTPFLIPV-TLTFSSET-MTYAEQEALLPPSEKQEPAR 292
           ED + L+ ++I  FT V+F ILL P  IP+ T  F++ T      +E LL   + Q+P +
Sbjct: 121 EDFIDLSHSIIIAFTVVLFAILLVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQ 180

Query: 293 S-EPD-GNEVIFSEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPR 352
           S  PD G E+IFSEVEDEK +  DLLPA+ER KRIAQLQA+L+QAAAEGAVRVKRR+GP 
Sbjct: 181 STTPDHGPELIFSEVEDEKPKEVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPH 240

Query: 353 RGEDFTLGQALIKADFWLIFVSLLLGSGTGLTVIDNLGQMSESLGYDNTHIFVSLISIWN 412
           RGEDFTL QAL+KADFWLIF SLLLGSG+GLTVIDNLGQMS+SLGYDNTH+FVS+ISIWN
Sbjct: 241 RGEDFTLTQALVKADFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHVFVSMISIWN 300

Query: 413 FLGRVGGGYLSEIVVRDFAYPRPIAMAIAQFLMIFGHLFIGMGWPGAMYIGTLITGLGYG 472
           FLGR+GGGY SE++VRD+AYPRP+A+A+AQ +M  GH+F   GWPGAM+IGTL+ GLGYG
Sbjct: 301 FLGRIGGGYFSELIVRDYAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYG 360

Query: 473 AHWAIVPATASELFGLKKFGALYNFLTLSNPMGSLIFSSLIASGIYDSEAEKQAHNHPPH 532
           AHWAIVPATASELFGLKKFGALYNFLTL+NP GSL+FS LIAS IYD EAE+QA      
Sbjct: 361 AHWAIVPATASELFGLKKFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQG---- 420

Query: 533 LQSSSSFLLSRLYADGPHECEGAICFFLTCMLMAGLCAIAGMLSLILVYRTKGVYSNLYG 592
                    S    D    C G+IC+FLT ++M+G C IA  LS+ILV RTK VY+NLYG
Sbjct: 421 ---------SLFNPDDVLRCRGSICYFLTSLIMSGFCLIAAALSMILVQRTKPVYTNLYG 470

BLAST of Bhi04G001992 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 505.0 bits (1299), Expect = 8.3e-143
Identity = 274/589 (46.52%), Postives = 381/589 (64.69%), Query Frame = 0

Query: 1   MGRWNEKLVAFFNNRWLVFVAAIWLQSCAGIGYLF-GSISPVIKTNLSYNQRQIARLGVA 60
           M  W  KL    N+RWLVFV A+W+QS AG+GYLF GS+SP IKT+L YNQ+QIA LGVA
Sbjct: 2   MTLWRHKLELLVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVA 61

Query: 61  KDLGDSVGILAATLSEILPFWGSLLVGAIHNFVGYGWIWLIVTGRAPVLPLWAMCILVFL 120
           K+LGD++G ++  LSE+ P W  LLVGA  N  GYG +WL+VTG+ P LPLW + + +F+
Sbjct: 62  KNLGDAIGFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFV 121

Query: 121 GTNGETYFNTVALVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIVHAPDSANLIFM 180
           GTNGETY+NT +LVSC+ NFP+SRGPVVGILKGF+GLSGAILTQ+Y + +    +++I M
Sbjct: 122 GTNGETYYNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYLMFNPSHDSSVILM 181

Query: 181 VAVGPALVAIGVMFFIRPV-AGHRQVRPSDSMSFTSVYGVCLLLAAYLMGVMLVEDLVTL 240
           VA+GP +V + ++F +RPV    R    SD + F ++YG C++LA YL+G+++++ +  +
Sbjct: 182 VALGPPVVVLALLFIVRPVERSCRTNLRSDDLRFLAIYGFCVVLAVYLLGLLVLQSVFDM 241

Query: 241 NPTVIAIFTGVMFVILLTPFLIPVTLTFSSETMTYAEQEALLPPSEKQEPARSEPDGNEV 300
             T+I     ++ + ++ P L+P +  F S                          GN V
Sbjct: 242 TQTIITTSGAILVIFMVVPVLVPFSSVFIS--------------------------GNNV 301

Query: 301 IFSEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQA 360
              + E+  S              + Q +AR L   ++     KR   P  GEDFTL QA
Sbjct: 302 TSVKPEEGTS-------------NVDQHEARTLIERSDRPPEKKR--APCIGEDFTLLQA 361

Query: 361 LIKADFWLIFVSLLLGSGTGLTVIDNLGQMSESLGYDNTHIFVSLISIWNFLGRVGGGYL 420
           L +ADFWLIF+SL+LG G+G+T+IDNLGQ+  SLGY NT IFVSLISI NFLGRV GGY 
Sbjct: 362 LGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYSNTKIFVSLISISNFLGRVAGGYF 421

Query: 421 SEIVVRDFAYPRPIAMAIAQFLMIFGHLFIGMGWPGAMYIGTLITGLGYGAHWAIVPATA 480
           SE+++R  + PR +AM++ Q +M  G ++  + WPG +Y+ T++ G+GYGAHWAI PA+ 
Sbjct: 422 SELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGMGYGAHWAIAPASV 481

Query: 481 SELFGLKKFGALYNFLTLSNPMGSLIFSSLIASGIYDSEAEKQAHNHPPHLQSSSSFLLS 540
           S++FGLK FG+LYNF   + P+GS +FS +IAS IYD  A KQA    P  ++ S     
Sbjct: 482 SDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARKQA---GPTTETESLV--- 534

Query: 541 RLYADGPHECEGAICFFLTCMLMAGLCAIAGMLSLILVYRTKGVYSNLY 588
                    C G++C+ +TC LM+ LC +A +LSL +VYRT+  Y  L+
Sbjct: 542 ---------CTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFYLRLH 534

BLAST of Bhi04G001992 vs. TAIR 10
Match: AT5G50520.1 (Major facilitator superfamily protein )

HSP 1 Score: 505.0 bits (1299), Expect = 8.3e-143
Identity = 274/589 (46.52%), Postives = 381/589 (64.69%), Query Frame = 0

Query: 1   MGRWNEKLVAFFNNRWLVFVAAIWLQSCAGIGYLF-GSISPVIKTNLSYNQRQIARLGVA 60
           M  W  KL    N+RWLVFV A+W+QS AG+GYLF GS+SP IKT+L YNQ+QIA LGVA
Sbjct: 2   MTLWRHKLELLVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVA 61

Query: 61  KDLGDSVGILAATLSEILPFWGSLLVGAIHNFVGYGWIWLIVTGRAPVLPLWAMCILVFL 120
           K+LGD++G ++  LSE+ P W  LLVGA  N  GYG +WL+VTG+ P LPLW + + +F+
Sbjct: 62  KNLGDAIGFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFV 121

Query: 121 GTNGETYFNTVALVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIVHAPDSANLIFM 180
           GTNGETY+NT +LVSC+ NFP+SRGPVVGILKGF+GLSGAILTQ+Y + +    +++I M
Sbjct: 122 GTNGETYYNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYLMFNPSHDSSVILM 181

Query: 181 VAVGPALVAIGVMFFIRPV-AGHRQVRPSDSMSFTSVYGVCLLLAAYLMGVMLVEDLVTL 240
           VA+GP +V + ++F +RPV    R    SD + F ++YG C++LA YL+G+++++ +  +
Sbjct: 182 VALGPPVVVLALLFIVRPVERSCRTNLRSDDLRFLAIYGFCVVLAVYLLGLLVLQSVFDM 241

Query: 241 NPTVIAIFTGVMFVILLTPFLIPVTLTFSSETMTYAEQEALLPPSEKQEPARSEPDGNEV 300
             T+I     ++ + ++ P L+P +  F S                          GN V
Sbjct: 242 TQTIITTSGAILVIFMVVPVLVPFSSVFIS--------------------------GNNV 301

Query: 301 IFSEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQA 360
              + E+  S              + Q +AR L   ++     KR   P  GEDFTL QA
Sbjct: 302 TSVKPEEGTS-------------NVDQHEARTLIERSDRPPEKKR--APCIGEDFTLLQA 361

Query: 361 LIKADFWLIFVSLLLGSGTGLTVIDNLGQMSESLGYDNTHIFVSLISIWNFLGRVGGGYL 420
           L +ADFWLIF+SL+LG G+G+T+IDNLGQ+  SLGY NT IFVSLISI NFLGRV GGY 
Sbjct: 362 LGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYSNTKIFVSLISISNFLGRVAGGYF 421

Query: 421 SEIVVRDFAYPRPIAMAIAQFLMIFGHLFIGMGWPGAMYIGTLITGLGYGAHWAIVPATA 480
           SE+++R  + PR +AM++ Q +M  G ++  + WPG +Y+ T++ G+GYGAHWAI PA+ 
Sbjct: 422 SELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGMGYGAHWAIAPASV 481

Query: 481 SELFGLKKFGALYNFLTLSNPMGSLIFSSLIASGIYDSEAEKQAHNHPPHLQSSSSFLLS 540
           S++FGLK FG+LYNF   + P+GS +FS +IAS IYD  A KQA    P  ++ S     
Sbjct: 482 SDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARKQA---GPTTETESLV--- 534

Query: 541 RLYADGPHECEGAICFFLTCMLMAGLCAIAGMLSLILVYRTKGVYSNLY 588
                    C G++C+ +TC LM+ LC +A +LSL +VYRT+  Y  L+
Sbjct: 542 ---------CTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFYLRLH 534

BLAST of Bhi04G001992 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 3.9e-28
Identity = 145/595 (24.37%), Postives = 259/595 (43.53%), Query Frame = 0

Query: 2   GRWNEKLVAFFNNRWLVFVAAIWLQSCAGIGYLFGSISPVIKTNLSYNQRQIARLGVAKD 61
           GRW          +W V VAAIW+Q+  G  + F + S  +K+ L  +Q ++  L VA D
Sbjct: 39  GRW---------RKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASD 98

Query: 62  LGDSVGILAATLSEILPFWGSLLVGAIHNFVGYGWIWLIVTG--RAPVLPLWAMCILVFL 121
           LG + G  +       P    L   A   FVGYG  WL++T     P   ++  C+L  L
Sbjct: 99  LGKAFGWSSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYSLVFLCCLLAGL 158

Query: 122 GTNGETYFNTVALVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIVHAPDSANLIFM 181
                 +FNT   + C+++FP +R   + +   F G+S A+ +  +  ++ P S+NL  +
Sbjct: 159 SI---CWFNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAIN-PSSSNLYLL 218

Query: 182 V-AVGPALVAIGVMF--FIRP---VAGHRQVRPSDSMSFTSVYGVCLLLAAYLMGVMLVE 241
           + ++ P +V+   ++    +P          R  DS  FT +  + ++ + +L+      
Sbjct: 219 LNSLVPLVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSST 278

Query: 242 DLVTLNPTVIAIFTGVMFVILLTPFLIPVTLTFSSETMTYAEQEALLPPSEKQEPARSEP 301
               LN      F G + V+L+ P   P+        + YA ++  LP       AR   
Sbjct: 279 SSARLN------FIGAV-VLLVFPLCAPL--------LVYA-RDYFLPVIN----ARLNH 338

Query: 302 DGNEVIFSEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDF 361
           + +  +   +++ K++   +                  +   E     K     R G++ 
Sbjct: 339 ESSGYVMLNIDELKNQKTSVSS----------------KTGYEHMGTAKEGNTVRLGDEH 398

Query: 362 TLGQALIKADFWLIFVSLLLGSGTGLTVIDNLGQMSESLGYDNTHIFVSLISIWNFLGRV 421
           +    + + +FWL +++   G   GL   +NLGQ+++SLG ++T + V++ S ++F GR+
Sbjct: 399 SFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNSTTL-VTIYSSFSFFGRL 458

Query: 422 GGGYLSEIVVRDFAYPRP--IAMAIAQFLMIFGHLFIGMGWPGAMYIGTLITGLGYGAHW 481
                 + + + F   R    A+A+    + F  L +      A+   T + GL  G  +
Sbjct: 459 LSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTATALIGLSSGFIF 518

Query: 482 AIVPATASELFGLKKFGALYNFLTLSNPMGSLIFSSLIASGIYDSEAEKQAHNHPPHLQS 541
           A   +  S+LFG    G  +N L  + P+GSL++   IA+ IY++ A             
Sbjct: 519 AAAVSITSDLFGPNSVGVNHNILITNIPIGSLLY-GYIAASIYEANASPD---------- 565

Query: 542 SSSFLLSRLYADGPHECEGAICFFLTCMLMAGLCAIAGMLSLILVYRTKGVYSNL 587
                ++ + +D    C G  C+F T +    L  +  + SL L  RTK VY  L
Sbjct: 579 -----ITPIVSDS-IVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYHRL 565

BLAST of Bhi04G001992 vs. ExPASy TrEMBL
Match: A0A5A7UN83 (Protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1154G00120 PE=4 SV=1)

HSP 1 Score: 1070.1 bits (2766), Expect = 3.3e-309
Identity = 546/596 (91.61%), Postives = 570/596 (95.64%), Query Frame = 0

Query: 1   MGRWNEKLVAFFNNRWLVFVAAIWLQSCAGIGYLFGSISPVIKTNLSYNQRQIARLGVAK 60
           MGRWN+KLVAF NNRWLVFVAAIWLQS AGIGYLFGSISPVIKTNLSYNQRQ++RLGVAK
Sbjct: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK 60

Query: 61  DLGDSVGILAATLSEILPFWGSLLVGAIHNFVGYGWIWLIVTGRAPVLPLWAMCILVFLG 120
           DLGDSVG LAATL+EILPFWGSLLVGAIHN VGYGW+WLIVTGRAPVLPLWAMC+LVF+G
Sbjct: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVALVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIVHAPDSANLIFMV 180
           TNGETYFNTV+LVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAI+H+PDSANLIFMV
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDSMSFTSVYGVCLLLAAYLMGVMLVEDLVTLNP 240
           AVGPALVAIGVMFFIRPVAGHRQVRPSD MSF+SVYGVCLLLAAYLMGVML+EDLVTL+P
Sbjct: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP 240

Query: 241 TVIAIFTGVMFVILLTPFLIPVTLTFSSETMTYAEQEALLPPSEKQEPARSEPDGNEVIF 300
           TVI IFT VMFVILLTPFLIPVTLTFSSET TYAEQEALLPPSEK+EPAR+EPDGNEVIF
Sbjct: 241 TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF 300

Query: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPA ERQKRIAQLQA+LLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFVSLLLGSGTGLTVIDNLGQMSESLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIF S LLGSGTGLTVIDNLGQMS+SLGYDNTHIFVSLISIWNFLGRVGGGYLSE
Sbjct: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420

Query: 421 IVVRDFAYPRPIAMAIAQFLMIFGHLFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDFAYPRPIAM IAQ LMIFGH+FIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFLTLSNPMGSLIFSSLIASGIYDSEAEKQAHNHPPHLQSSSSFLLSRL 540
           LFGLKKFGALYNF+TLS PMGSLIFS LIAS IYDSEAEKQA NH    QSSSS   +RL
Sbjct: 481 LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL 540

Query: 541 YADGPHECEGAICFFLTCMLMAGLCAIAGMLSLILVYRTKGVYSNLYGKSRTSTLS 597
           YA+GPH+CEGAICFFLTCM+MAG CAIAG+LSLILVYRTKGVY NLYGKSRTSTLS
Sbjct: 541 YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS 596

BLAST of Bhi04G001992 vs. ExPASy TrEMBL
Match: A0A1S3B357 (protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485475 PE=4 SV=1)

HSP 1 Score: 1070.1 bits (2766), Expect = 3.3e-309
Identity = 546/596 (91.61%), Postives = 570/596 (95.64%), Query Frame = 0

Query: 1   MGRWNEKLVAFFNNRWLVFVAAIWLQSCAGIGYLFGSISPVIKTNLSYNQRQIARLGVAK 60
           MGRWN+KLVAF NNRWLVFVAAIWLQS AGIGYLFGSISPVIKTNLSYNQRQ++RLGVAK
Sbjct: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK 60

Query: 61  DLGDSVGILAATLSEILPFWGSLLVGAIHNFVGYGWIWLIVTGRAPVLPLWAMCILVFLG 120
           DLGDSVG LAATL+EILPFWGSLLVGAIHN VGYGW+WLIVTGRAPVLPLWAMC+LVF+G
Sbjct: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVALVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIVHAPDSANLIFMV 180
           TNGETYFNTV+LVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAI+H+PDSANLIFMV
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDSMSFTSVYGVCLLLAAYLMGVMLVEDLVTLNP 240
           AVGPALVAIGVMFFIRPVAGHRQVRPSD MSF+SVYGVCLLLAAYLMGVML+EDLVTL+P
Sbjct: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP 240

Query: 241 TVIAIFTGVMFVILLTPFLIPVTLTFSSETMTYAEQEALLPPSEKQEPARSEPDGNEVIF 300
           TVI IFT VMFVILLTPFLIPVTLTFSSET TYAEQEALLPPSEK+EPAR+EPDGNEVIF
Sbjct: 241 TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF 300

Query: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPA ERQKRIAQLQA+LLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFVSLLLGSGTGLTVIDNLGQMSESLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIF S LLGSGTGLTVIDNLGQMS+SLGYDNTHIFVSLISIWNFLGRVGGGYLSE
Sbjct: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420

Query: 421 IVVRDFAYPRPIAMAIAQFLMIFGHLFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDFAYPRPIAM IAQ LMIFGH+FIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFLTLSNPMGSLIFSSLIASGIYDSEAEKQAHNHPPHLQSSSSFLLSRL 540
           LFGLKKFGALYNF+TLS PMGSLIFS LIAS IYDSEAEKQA NH    QSSSS   +RL
Sbjct: 481 LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL 540

Query: 541 YADGPHECEGAICFFLTCMLMAGLCAIAGMLSLILVYRTKGVYSNLYGKSRTSTLS 597
           YA+GPH+CEGAICFFLTCM+MAG CAIAG+LSLILVYRTKGVY NLYGKSRTSTLS
Sbjct: 541 YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS 596

BLAST of Bhi04G001992 vs. ExPASy TrEMBL
Match: A0A0A0LQ60 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G406130 PE=4 SV=1)

HSP 1 Score: 1052.7 bits (2721), Expect = 5.6e-304
Identity = 537/596 (90.10%), Postives = 562/596 (94.30%), Query Frame = 0

Query: 1   MGRWNEKLVAFFNNRWLVFVAAIWLQSCAGIGYLFGSISPVIKTNLSYNQRQIARLGVAK 60
           MGRWN+KLVAF NNRWLVFVAAIWLQS AGIGYLFGSISP+IKTNLSYNQRQI+RLGVAK
Sbjct: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAK 60

Query: 61  DLGDSVGILAATLSEILPFWGSLLVGAIHNFVGYGWIWLIVTGRAPVLPLWAMCILVFLG 120
           DLGDSVG LAATL+EILPFWGSLLVGAIHNFVGYGW+WLIVTGRAPVLPLWAMC LVF+G
Sbjct: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIG 120

Query: 121 TNGETYFNTVALVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIVHAPDSANLIFMV 180
           TNGETYFNTV+LVSCVQNFPKSRGPVVGILKGFAGLSGAILTQ YAI H+P+SANLIFMV
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMV 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDSMSFTSVYGVCLLLAAYLMGVMLVEDLVTLNP 240
           AVGPALVAIGVMFFIRPVAGHRQVRPSD MSFTSVYGVCLLLAAYLMGVML+EDLVTL+P
Sbjct: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSP 240

Query: 241 TVIAIFTGVMFVILLTPFLIPVTLTFSSETMTYAEQEALLPPSEKQEPARSEPDGNEVIF 300
            VI IFT VMFVILLTPF IPV+LT SSE  TYAEQEALLPPSEK+EPAR+EPDGNEVIF
Sbjct: 241 IVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEVIF 300

Query: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPA ERQKRIAQLQA+LLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFVSLLLGSGTGLTVIDNLGQMSESLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIF S LLGSGTGLTVIDNLGQMS+SLGYDNTHIFVSLISIWNFLGRVGGGYLSE
Sbjct: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420

Query: 421 IVVRDFAYPRPIAMAIAQFLMIFGHLFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDFAYPRPIAM IAQ LMIFGH+FIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFLTLSNPMGSLIFSSLIASGIYDSEAEKQAHNHPPHLQSSSSFLLSRL 540
           LFGLKKFGALYNF+TLS PMGSL+FS LIAS IYDSEAEKQA NH    QSSSSF  +RL
Sbjct: 481 LFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRL 540

Query: 541 YADGPHECEGAICFFLTCMLMAGLCAIAGMLSLILVYRTKGVYSNLYGKSRTSTLS 597
           Y +GPH+CEGAICFFLTCM+M G CAIA +LSLILV+RTKGVY NLYGKSRTSTLS
Sbjct: 541 YTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSRTSTLS 596

BLAST of Bhi04G001992 vs. ExPASy TrEMBL
Match: A0A6J1JLV2 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111487059 PE=4 SV=1)

HSP 1 Score: 1049.7 bits (2713), Expect = 4.7e-303
Identity = 540/596 (90.60%), Postives = 564/596 (94.63%), Query Frame = 0

Query: 1   MGRWNEKLVAFFNNRWLVFVAAIWLQSCAGIGYLFGSISPVIKTNLSYNQRQIARLGVAK 60
           MGR NEKLVAF NNRWLVFVAAIW+QSCAGIGYLFGSISPVIKTNLSYNQ+QIARLGVAK
Sbjct: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60

Query: 61  DLGDSVGILAATLSEILPFWGSLLVGAIHNFVGYGWIWLIVTGRAPVLPLWAMCILVFLG 120
           DLGDSVGILA TLSEILPFWG+LLVGA++NF+GYGW+WLIVTGRAPVLPLWAMC+LVF+G
Sbjct: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVALVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIVHAPDSANLIFMV 180
           TNGETYFNTV+LVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAI+H PDSANLIFM+
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDSMSFTSVYGVCLLLAAYLMGVMLVEDLVTLNP 240
           AVGPALVAIG+MFFIRPVAGHRQVRPSD MSF+SVYGVCLLLAAYLMGVMLVEDLV L+P
Sbjct: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240

Query: 241 TVIAIFTGVMFVILLTPFLIPVTLTFSSETMTYAEQEALLPPSEKQEPARSEPDGNEVIF 300
           TVIAIFT VMFVILLTPFLIPV LTFSSETM Y EQEALL  S KQEPARSEPDG+EVIF
Sbjct: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVIF 300

Query: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFVSLLLGSGTGLTVIDNLGQMSESLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIF SLLLGSGTGLTVIDNLGQMS+SLGYDNTHIFVSLISIWNFLGRVGGGY SE
Sbjct: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420

Query: 421 IVVRDFAYPRPIAMAIAQFLMIFGHLFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRD+AYPRPIAMA AQFLMIFGH+FIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFLTLSNPMGSLIFSSLIASGIYDSEAEKQAHNHPPHLQSSSSFLLSRL 540
           LFGLKKFGALYNFLTLS PMGSLIFS LIAS IYDSEAEKQAHN    LQSSSS   SRL
Sbjct: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540

Query: 541 YADGPHECEGAICFFLTCMLMAGLCAIAGMLSLILVYRTKGVYSNLYGKSRTSTLS 597
           + D P +C+GAICFFLTCM+MAG CAIAGMLSLILV+RTKGVY NLYGKSR STLS
Sbjct: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 596

BLAST of Bhi04G001992 vs. ExPASy TrEMBL
Match: A0A6J1E533 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111430673 PE=4 SV=1)

HSP 1 Score: 1041.6 bits (2692), Expect = 1.3e-300
Identity = 536/596 (89.93%), Postives = 562/596 (94.30%), Query Frame = 0

Query: 1   MGRWNEKLVAFFNNRWLVFVAAIWLQSCAGIGYLFGSISPVIKTNLSYNQRQIARLGVAK 60
           MGR NEKLVAF NNRWLVFVAAIW+QSCAGIGYLFGSISPVIKTNLSYNQ+QIARLGVAK
Sbjct: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60

Query: 61  DLGDSVGILAATLSEILPFWGSLLVGAIHNFVGYGWIWLIVTGRAPVLPLWAMCILVFLG 120
           DLGDSVGILA TLSEILPFWG+LLVGA++NF+GYGW+WLIVTGRAPVLPLWAMC+LVF+G
Sbjct: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVALVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIVHAPDSANLIFMV 180
           TNGETYFNTV+LVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAI+H PDSANLIFM+
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDSMSFTSVYGVCLLLAAYLMGVMLVEDLVTLNP 240
           AVGPALVAIG+MFFIRPVAGHRQVRPSD +SF+SVYGVCLLLAAYLMGVMLVEDLV L+P
Sbjct: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGVSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240

Query: 241 TVIAIFTGVMFVILLTPFLIPVTLTFSSETMTYAEQEALLPPSEKQEPARSEPDGNEVIF 300
           TVIAIFT VMFVILLTPFLIPV LTFSSET  Y EQEALL  S KQEPARSEPDG+EVIF
Sbjct: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETTAYPEQEALLQQSPKQEPARSEPDGHEVIF 300

Query: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPA ERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFVSLLLGSGTGLTVIDNLGQMSESLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIF SLLLGSGTGLTVIDNLGQMS+SLGY+NTHIFVSLISIWNFLGRVGGGY SE
Sbjct: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYNNTHIFVSLISIWNFLGRVGGGYFSE 420

Query: 421 IVVRDFAYPRPIAMAIAQFLMIFGHLFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRD+AYPRPIAMA AQFLMIFGH+FIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFLTLSNPMGSLIFSSLIASGIYDSEAEKQAHNHPPHLQSSSSFLLSRL 540
           LFGLKKFGALYNFLTLS PMGSLIFS LIAS IYDSEAEKQAHN    LQSSSS   SRL
Sbjct: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540

Query: 541 YADGPHECEGAICFFLTCMLMAGLCAIAGMLSLILVYRTKGVYSNLYGKSRTSTLS 597
           + D P +C+GAICFFLTCM+MAG CAIAGMLSLILV+RTKGVY NLYGKSR STLS
Sbjct: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 596

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G14120.13.5e-23469.27Major facilitator superfamily protein [more]
AT3G01930.21.6e-23169.32Major facilitator superfamily protein [more]
AT3G01930.18.8e-17767.49Major facilitator superfamily protein [more]
AT5G50630.18.3e-14346.52Major facilitator superfamily protein [more]
AT5G50520.18.3e-14346.52Major facilitator superfamily protein [more]
Match NameE-valueIdentityDescription
F4I9E13.9e-2824.37Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A5A7UN833.3e-30991.61Protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3B3573.3e-30991.61protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A0A0LQ605.6e-30490.10Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G40613... [more]
A0A6J1JLV24.7e-30390.60protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111487... [more]
A0A6J1E5331.3e-30089.93protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111430673... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 15..262
e-value: 1.2E-88
score: 296.9
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 299..524
e-value: 2.1E-10
score: 41.9
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 211..581
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 9..202
NoneNo IPR availablePANTHERPTHR21576:SF136PROTEIN NUCLEAR FUSION DEFECTIVE 4-LIKEcoord: 3..592
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 3..592
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..28
score: 6.0
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 15..568
e-value: 1.54833E-113
score: 342.309

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi04M001992Bhi04M001992mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane