Bhi04G001375 (gene) Wax gourd (B227) v1

BLAST of Bhi04G001375 vs. TAIR 10
Match: AT3G53010.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 219.9 bits (559), Expect = 2.5e-57
Identity = 124/262 (47.33%), Postives = 155/262 (59.16%), Query Frame = 0

Query: 4   PIPNSKVRTSPNTIFILAGQSNMAGRGGILQDPTTNKMVWDGYIPPECQSNESIFRLTAD 63
           P   S+  T   +IFILAGQSNMAGRGG+  D  TN  VWDG IPPEC+SN SI RLT+ 
Sbjct: 18  PHLQSQTITRNISIFILAGQSNMAGRGGVYNDTATNTTVWDGVIPPECRSNPSILRLTSK 77

Query: 64  LVWEQAHEPLHWDIDVVKTNGIGPGMAFANELLAIAGDRVGVIGLVPCAIGGSHLKEWVK 123
           L W++A EPLH DID+ KTNG+GPGM FAN ++    +R G +GLVPC+IGG+ L +W K
Sbjct: 78  LEWKEAKEPLHVDIDINKTNGVGPGMPFANRVV----NRFGQVGLVPCSIGGTKLSQWQK 137

Query: 124 GTDRYANLVERIKA--SEKNGGKVQGFLWYQGECDDAVEDEAMCYERELTKFFTDLRADV 183
           G   Y   V+R KA  +   GG  +  LWYQGE D     +A  Y++ L KFF+DLR D+
Sbjct: 138 GEFLYEETVKRAKAAMASGGGGSYRAVLWYQGESDTVDMVDASVYKKRLVKFFSDLRNDL 197

Query: 184 NHPDLPFIVVKIVTHDFFISPNISFKEEVWKALEAVTHKLPNVRMVDGPMAVGNFDEGLN 243
            HP+LP I V + T      P   + + V KA   +   L NV  VD           L 
Sbjct: 198 QHPNLPIIQVALATG---AGP---YLDAVRKA--QLKTDLENVYCVDAR------GLPLE 257

Query: 244 EDKGHLNVKSEVKLGKMFAHSF 264
            D  HL   S+V+LG M A SF
Sbjct: 258 PDGLHLTTSSQVQLGHMIAESF 261

BLAST of Bhi04G001375 vs. TAIR 10
Match: AT4G34215.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 211.5 bits (537), Expect = 8.7e-55
Identity = 115/255 (45.10%), Postives = 148/255 (58.04%), Query Frame = 0

Query: 14  PNTIFILAGQSNMAGRGGILQDPTTNKMVWDGYIPPECQSNESIFRLTADLVWEQAHEPL 73
           PN IFIL+GQSNMAGRGG+ +D   N+ VWD  +PPEC  N SI RL+ADL WE+AHEPL
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 74  HWDIDVVKTNGIGPGMAFANELLAIAGDRVGVIGLVPCAIGGSHLKEWVKGTDRYANLVE 133
           H DID  K  G+GPGMAFAN +         VIGLVPCA GG+ +KEW +G+  Y  +V+
Sbjct: 81  HVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSHLYERMVK 140

Query: 134 RIKASEKNGGKVQGFLWYQGECDDAVEDEAMCYERELTKFFTDLRADVNHPDLPFIVVKI 193
           R + S K GG+++  LWYQGE D     +A  Y   + +   +LR D+N P LP I V I
Sbjct: 141 RTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPIIQVAI 200

Query: 194 VTHDFFISPNISFKEEVWKALEA-VTHKLPNVRMVDGPMAVGNFDEGLNEDKGHLNVKSE 253
            +   +I           K  EA +  KL NV  VD           L  D  HL  +++
Sbjct: 201 ASGGGYID----------KVREAQLGLKLSNVVCVDAK------GLPLKSDNLHLTTEAQ 259

Query: 254 VKLGKMFAHSFYSNF 268
           V+LG   A ++ SNF
Sbjct: 261 VQLGLSLAQAYLSNF 259

BLAST of Bhi04G001375 vs. TAIR 10
Match: AT4G34215.2 (Domain of unknown function (DUF303) )

HSP 1 Score: 211.5 bits (537), Expect = 8.7e-55
Identity = 115/255 (45.10%), Postives = 148/255 (58.04%), Query Frame = 0

Query: 14  PNTIFILAGQSNMAGRGGILQDPTTNKMVWDGYIPPECQSNESIFRLTADLVWEQAHEPL 73
           PN IFIL+GQSNMAGRGG+ +D   N+ VWD  +PPEC  N SI RL+ADL WE+AHEPL
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 74  HWDIDVVKTNGIGPGMAFANELLAIAGDRVGVIGLVPCAIGGSHLKEWVKGTDRYANLVE 133
           H DID  K  G+GPGMAFAN +         VIGLVPCA GG+ +KEW +G+  Y  +V+
Sbjct: 81  HVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSHLYERMVK 140

Query: 134 RIKASEKNGGKVQGFLWYQGECDDAVEDEAMCYERELTKFFTDLRADVNHPDLPFIVVKI 193
           R + S K GG+++  LWYQGE D     +A  Y   + +   +LR D+N P LP I V I
Sbjct: 141 RTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPIIQVAI 200

Query: 194 VTHDFFISPNISFKEEVWKALEA-VTHKLPNVRMVDGPMAVGNFDEGLNEDKGHLNVKSE 253
            +   +I           K  EA +  KL NV  VD           L  D  HL  +++
Sbjct: 201 ASGGGYID----------KVREAQLGLKLSNVVCVDAK------GLPLKSDNLHLTTEAQ 259

Query: 254 VKLGKMFAHSFYSNF 268
           V+LG   A ++ SNF
Sbjct: 261 VQLGLSLAQAYLSNF 259

BLAST of Bhi04G001375 vs. ExPASy Swiss-Prot
Match: Q8L9J9 (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 211.5 bits (537), Expect = 1.2e-53
Identity = 115/255 (45.10%), Postives = 148/255 (58.04%), Query Frame = 0

Query: 14  PNTIFILAGQSNMAGRGGILQDPTTNKMVWDGYIPPECQSNESIFRLTADLVWEQAHEPL 73
           PN IFIL+GQSNMAGRGG+ +D   N+ VWD  +PPEC  N SI RL+ADL WE+AHEPL
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 74  HWDIDVVKTNGIGPGMAFANELLAIAGDRVGVIGLVPCAIGGSHLKEWVKGTDRYANLVE 133
           H DID  K  G+GPGMAFAN +         VIGLVPCA GG+ +KEW +G+  Y  +V+
Sbjct: 81  HVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSHLYERMVK 140

Query: 134 RIKASEKNGGKVQGFLWYQGECDDAVEDEAMCYERELTKFFTDLRADVNHPDLPFIVVKI 193
           R + S K GG+++  LWYQGE D     +A  Y   + +   +LR D+N P LP I V I
Sbjct: 141 RTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPIIQVAI 200

Query: 194 VTHDFFISPNISFKEEVWKALEA-VTHKLPNVRMVDGPMAVGNFDEGLNEDKGHLNVKSE 253
            +   +I           K  EA +  KL NV  VD           L  D  HL  +++
Sbjct: 201 ASGGGYID----------KVREAQLGLKLSNVVCVDAK------GLPLKSDNLHLTTEAQ 259

Query: 254 VKLGKMFAHSFYSNF 268
           V+LG   A ++ SNF
Sbjct: 261 VQLGLSLAQAYLSNF 259

BLAST of Bhi04G001375 vs. ExPASy TrEMBL
Match: A0A5D3CGI9 (Putative carbohydrate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold874G00750 PE=4 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 1.4e-137
Identity = 235/272 (86.40%), Postives = 248/272 (91.18%), Query Frame = 0

Query: 1   MTDPIPNSKVRTSPNTIFILAGQSNMAGRGGILQDPTTNKMVWDGYIPPECQSNESIFRL 60
           MTD I + K  TSPN IFILAGQSNMAGRGG+ QDPTT KMVWDGYIP ECQSN+SIFRL
Sbjct: 1   MTDSILSPKATTSPNNIFILAGQSNMAGRGGVSQDPTTEKMVWDGYIPLECQSNDSIFRL 60

Query: 61  TADLVWEQAHEPLHWDIDVVKTNGIGPGMAFANELLAIAGDRVGVIGLVPCAIGGSHLKE 120
            AD+VWE+AHEPLHWDIDVVKTNGIGPGM FANELLAI G R G IGLVPCAIGGSHLKE
Sbjct: 61  NADMVWEKAHEPLHWDIDVVKTNGIGPGMPFANELLAIGGKRAGAIGLVPCAIGGSHLKE 120

Query: 121 WVKGTDRYANLVERIKASEKNGGKVQGFLWYQGECDDAVEDEAMCYERELTKFFTDLRAD 180
           WVKGTDRY NLVERI+ASEK+GGKV+GFLWYQGE D AVE+EAMCYERELTKFF DLRAD
Sbjct: 121 WVKGTDRYDNLVERIRASEKDGGKVKGFLWYQGESDAAVEEEAMCYERELTKFFIDLRAD 180

Query: 181 VNHPDLPFIVVKIVTHDFFISPNISFKEEVWKALEAVTHKLPNVRMVDGPMAVGNFDEGL 240
            NHPDLP I+VKIVTHDFF+SPNISFKEEV KALEAVTH LPNV MVDGPMAVGNFD+GL
Sbjct: 181 TNHPDLPIIMVKIVTHDFFLSPNISFKEEVLKALEAVTHNLPNVTMVDGPMAVGNFDDGL 240

Query: 241 NEDKGHLNVKSEVKLGKMFAHSFYSNFAHNFI 273
           NEDKGHLNVKSEVKLGKMFAHSFYSNFAHNF+
Sbjct: 241 NEDKGHLNVKSEVKLGKMFAHSFYSNFAHNFL 272

BLAST of Bhi04G001375 vs. ExPASy TrEMBL
Match: A0A1S3B3Y0 (probable carbohydrate esterase At4g34215 OS=Cucumis melo OX=3656 GN=LOC103485730 PE=4 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 1.4e-137
Identity = 235/272 (86.40%), Postives = 248/272 (91.18%), Query Frame = 0

Query: 1   MTDPIPNSKVRTSPNTIFILAGQSNMAGRGGILQDPTTNKMVWDGYIPPECQSNESIFRL 60
           MTD I + K  TSPN IFILAGQSNMAGRGG+ QDPTT KMVWDGYIP ECQSN+SIFRL
Sbjct: 1   MTDSILSPKATTSPNNIFILAGQSNMAGRGGVSQDPTTEKMVWDGYIPLECQSNDSIFRL 60

Query: 61  TADLVWEQAHEPLHWDIDVVKTNGIGPGMAFANELLAIAGDRVGVIGLVPCAIGGSHLKE 120
            AD+VWE+AHEPLHWDIDVVKTNGIGPGM FANELLAI G R G IGLVPCAIGGSHLKE
Sbjct: 61  NADMVWEKAHEPLHWDIDVVKTNGIGPGMPFANELLAIGGKRAGAIGLVPCAIGGSHLKE 120

Query: 121 WVKGTDRYANLVERIKASEKNGGKVQGFLWYQGECDDAVEDEAMCYERELTKFFTDLRAD 180
           WVKGTDRY NLVERI+ASEK+GGKV+GFLWYQGE D AVE+EAMCYERELTKFF DLRAD
Sbjct: 121 WVKGTDRYDNLVERIRASEKDGGKVKGFLWYQGESDAAVEEEAMCYERELTKFFIDLRAD 180

Query: 181 VNHPDLPFIVVKIVTHDFFISPNISFKEEVWKALEAVTHKLPNVRMVDGPMAVGNFDEGL 240
            NHPDLP I+VKIVTHDFF+SPNISFKEEV KALEAVTH LPNV MVDGPMAVGNFD+GL
Sbjct: 181 TNHPDLPIIMVKIVTHDFFLSPNISFKEEVLKALEAVTHNLPNVTMVDGPMAVGNFDDGL 240

Query: 241 NEDKGHLNVKSEVKLGKMFAHSFYSNFAHNFI 273
           NEDKGHLNVKSEVKLGKMFAHSFYSNFAHNF+
Sbjct: 241 NEDKGHLNVKSEVKLGKMFAHSFYSNFAHNFL 272

BLAST of Bhi04G001375 vs. ExPASy TrEMBL
Match: A0A0A0K9J9 (SASA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G058180 PE=4 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 2.1e-136
Identity = 232/273 (84.98%), Postives = 249/273 (91.21%), Query Frame = 0

Query: 1   MTDPIPNSKVRTSPNTIFILAGQSNMAGRGGILQDPTTNKMVWDGYIPPECQSNESIFRL 60
           MTD I + K  TSPN IFILAGQSNMAGRGG+  DPTT+KMVWDGYIP EC+SN+SIFRL
Sbjct: 1   MTDSILSPKATTSPNNIFILAGQSNMAGRGGVSLDPTTDKMVWDGYIPLECESNDSIFRL 60

Query: 61  TADLVWEQAHEPLHWDIDVVKTNGIGPGMAFANELLAIAGDRVGVIGLVPCAIGGSHLKE 120
            AD+VWEQAHEPLHWDIDVVKTNGIGPGMAFANELLAI G R+G IGLVPCAIGGSHLKE
Sbjct: 61  NADMVWEQAHEPLHWDIDVVKTNGIGPGMAFANELLAIGGKRIGAIGLVPCAIGGSHLKE 120

Query: 121 WVKGTDRYANLVERIKASEKNGGKVQGFLWYQGECDDAVEDEAMCYERELTKFFTDLRAD 180
           WVKGT+RY NLVERI+ASEKNGG VQG LWYQGE D AVE+EAMCYERELTKFF DLRAD
Sbjct: 121 WVKGTNRYDNLVERIRASEKNGGTVQGILWYQGESDAAVEEEAMCYERELTKFFIDLRAD 180

Query: 181 VNHPDLPFIVVKIVTHDFFISPNISFKEEVWKALEAVTHKLPNVRMVDGPMAVGNFDEGL 240
            NHP+LP I+VK+VTHDFF+SPNISFKEEV  ALEAVTH+LPNV MVDGPMAVGNFD+GL
Sbjct: 181 TNHPELPIILVKLVTHDFFLSPNISFKEEVCNALEAVTHRLPNVTMVDGPMAVGNFDDGL 240

Query: 241 NEDKGHLNVKSEVKLGKMFAHSFYSNFAHNFIS 274
           NEDKGHLNVKSEVKLGKMFAHSFYSNFAHNF+S
Sbjct: 241 NEDKGHLNVKSEVKLGKMFAHSFYSNFAHNFLS 273

BLAST of Bhi04G001375 vs. ExPASy TrEMBL
Match: A0A6J1K1G7 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111489769 PE=4 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 4.4e-123
Identity = 212/262 (80.92%), Postives = 232/262 (88.55%), Query Frame = 0

Query: 12  TSPNTIFILAGQSNMAGRGGILQDPTTNKMVWDGYIPPECQSNESIFRLTADLVWEQAHE 71
           TSP  IFILAGQSNMAGRGG+ +DPTT+K VWDGYIPPE Q N+SIFR TAD+VWEQA E
Sbjct: 4   TSPANIFILAGQSNMAGRGGVSKDPTTDKNVWDGYIPPESQPNQSIFRFTADMVWEQARE 63

Query: 72  PLHWDIDVVKTNGIGPGMAFANELLAIAGDRVGVIGLVPCAIGGSHLKEWVKGTDRYANL 131
           PLHWDIDVVKTNG+GPGM FANELLA AG  +G IGLVPCAIGGSHL+EWVKGTDRY  L
Sbjct: 64  PLHWDIDVVKTNGVGPGMPFANELLAKAGPSIGTIGLVPCAIGGSHLREWVKGTDRYTKL 123

Query: 132 VERIKASEKNGGKVQGFLWYQGECDDAVEDEAMCYERELTKFFTDLRADVNHPDLPFIVV 191
           VER+K SE++GGKV+GF WYQGE D AVE+EA  YEREL+KFFTDLRADVNHPDLP I+V
Sbjct: 124 VERMKRSEEHGGKVKGFFWYQGESDAAVEEEAKSYERELSKFFTDLRADVNHPDLPIILV 183

Query: 192 KIVTHDFFISPNISFKEEVWKALEAVTHKLPNVRMVDGPMAVGNFDEGLNEDKGHLNVKS 251
           KIVTHDFFISP+  FK+EVW A EAVT KLPNVRMVDG +AVGNFDEGLNED+GHLNVKS
Sbjct: 184 KIVTHDFFISPDFEFKDEVWNAQEAVTQKLPNVRMVDGRVAVGNFDEGLNEDRGHLNVKS 243

Query: 252 EVKLGKMFAHSFYSNFAHNFIS 274
           EV LGKMFAHS+YSNFAH FIS
Sbjct: 244 EVNLGKMFAHSYYSNFAH-FIS 264

BLAST of Bhi04G001375 vs. ExPASy TrEMBL
Match: A0A6J1FFF9 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111445241 PE=4 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 2.2e-122
Identity = 210/262 (80.15%), Postives = 232/262 (88.55%), Query Frame = 0

Query: 12  TSPNTIFILAGQSNMAGRGGILQDPTTNKMVWDGYIPPECQSNESIFRLTADLVWEQAHE 71
           TSP  IFILAGQSNMAGRGG+ +DPTT+K VWDGYIPPE Q N+SIFR TAD+VWEQA E
Sbjct: 4   TSPANIFILAGQSNMAGRGGVSKDPTTDKNVWDGYIPPESQPNQSIFRFTADMVWEQARE 63

Query: 72  PLHWDIDVVKTNGIGPGMAFANELLAIAGDRVGVIGLVPCAIGGSHLKEWVKGTDRYANL 131
           PLHWDIDVVKTNG+GPGM FANELLA AG  +G IGLVPCAIGGSHL+EWVKGT+RY  L
Sbjct: 64  PLHWDIDVVKTNGVGPGMPFANELLAKAGPSIGTIGLVPCAIGGSHLREWVKGTNRYTKL 123

Query: 132 VERIKASEKNGGKVQGFLWYQGECDDAVEDEAMCYERELTKFFTDLRADVNHPDLPFIVV 191
           VER+K SE++GGKV+GF WYQGE D AVE+EA  YEREL+KFFTDLRAD+NHPDLP I+V
Sbjct: 124 VERMKRSEEHGGKVKGFFWYQGESDAAVEEEAKSYERELSKFFTDLRADMNHPDLPIILV 183

Query: 192 KIVTHDFFISPNISFKEEVWKALEAVTHKLPNVRMVDGPMAVGNFDEGLNEDKGHLNVKS 251
           KIVTHDFFISP+  FKEEVW A EAVT KLPN+RMVDG +AVGNFDEGLNED+GHLNVKS
Sbjct: 184 KIVTHDFFISPDFEFKEEVWNAQEAVTQKLPNIRMVDGRVAVGNFDEGLNEDRGHLNVKS 243

Query: 252 EVKLGKMFAHSFYSNFAHNFIS 274
           EV LGKMFAHS+YSNFAH FIS
Sbjct: 244 EVNLGKMFAHSYYSNFAH-FIS 264