Bhi04G000830 (gene) Wax gourd (B227) v1

Overview


Name	Bhi04G000830
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	gibberellin 3-beta-dioxygenase 1-like
Location	chr4: 25779903 .. 25780394 (-)
RNA-Seq Expression	Bhi04G000830
Synteny	Bhi04G000830

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGAAGATGTTGACTTCAACCTGCGACTCCGGCGGAAAACCCTACATGGCTCTCCGCCTGAACTCATATCCACCGTGCCCCGATCCAAGTCGAGTCGTCGGCCTCGCCGCCCACACTGACACCTCCCTCTTAACCATCATTCATCAGGTACGCAGTGATGGGCTCCAGGTCTTCAACGACGGAGCAGGTTGGGTTCCGTTGTCTCCGATGAGTGGTACTCTCATCGTCAACGTTGGCGATATCCTACATATTCTTTCAAATGGGCGGTTTCCGAGCATTCTTCACCGGGTGACGATACAAGAAAATAAGGAGCATAGATATTCATTGGGATATTTCTATGATCCACCAGCTGGTTTATACATTTCACCTTATTGTAAGCCATTGAGTGAATCTCCACAGATTCCTCTTTATAGATGTGTGAATGTGCAAGAATATTTCACCATCAAGGCCAGCAACAAGGGAAAAGGACTTTCTGCCATAAAAATATGA

mRNA sequence

ATGGTGAAGATGTTGACTTCAACCTGCGACTCCGGCGGAAAACCCTACATGGCTCTCCGCCTGAACTCATATCCACCGTGCCCCGATCCAAGTCGAGTCGTCGGCCTCGCCGCCCACACTGACACCTCCCTCTTAACCATCATTCATCAGGTACGCAGTGATGGGCTCCAGGTCTTCAACGACGGAGCAGGTTGGGTTCCGTTGTCTCCGATGAGTGGTACTCTCATCGTCAACGTTGGCGATATCCTACATATTCTTTCAAATGGGCGGTTTCCGAGCATTCTTCACCGGGTGACGATACAAGAAAATAAGGAGCATAGATATTCATTGGGATATTTCTATGATCCACCAGCTGGTTTATACATTTCACCTTATTGTAAGCCATTGAGTGAATCTCCACAGATTCCTCTTTATAGATGTGTGAATGTGCAAGAATATTTCACCATCAAGGCCAGCAACAAGGGAAAAGGACTTTCTGCCATAAAAATATGA

Coding sequence (CDS)

ATGGTGAAGATGTTGACTTCAACCTGCGACTCCGGCGGAAAACCCTACATGGCTCTCCGCCTGAACTCATATCCACCGTGCCCCGATCCAAGTCGAGTCGTCGGCCTCGCCGCCCACACTGACACCTCCCTCTTAACCATCATTCATCAGGTACGCAGTGATGGGCTCCAGGTCTTCAACGACGGAGCAGGTTGGGTTCCGTTGTCTCCGATGAGTGGTACTCTCATCGTCAACGTTGGCGATATCCTACATATTCTTTCAAATGGGCGGTTTCCGAGCATTCTTCACCGGGTGACGATACAAGAAAATAAGGAGCATAGATATTCATTGGGATATTTCTATGATCCACCAGCTGGTTTATACATTTCACCTTATTGTAAGCCATTGAGTGAATCTCCACAGATTCCTCTTTATAGATGTGTGAATGTGCAAGAATATTTCACCATCAAGGCCAGCAACAAGGGAAAAGGACTTTCTGCCATAAAAATATGA

Protein sequence

MVKMLTSTCDSGGKPYMALRLNSYPPCPDPSRVVGLAAHTDTSLLTIIHQVRSDGLQVFNDGAGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVTIQENKEHRYSLGYFYDPPAGLYISPYCKPLSESPQIPLYRCVNVQEYFTIKASNKGKGLSAIKI

Homology

[Close]

BLAST of Bhi04G000830 vs. TAIR 10
Match: AT1G80340.1 (gibberellin 3-oxidase 2 )

HSP 1 Score: 145.6 bits (366), Expect = 3.5e-35
Identity = 68/144 (47.22%), Postives = 99/144 (68.75%), Query Frame = 0


Query: 19  LRLNSYPPCPDPSRVVGLAAHTDTSLLTIIHQVRSDGLQVFNDGAGWVPLSPMSGTLIVN 78
           ++LN YP CP+P R +GLAAHTD++L+TI++Q  + GLQVF D  GWV   P+ G+L+VN
Sbjct: 205 IQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGWVTAPPVPGSLVVN 264

Query: 79  VGDILHILSNGRFPSILHRVTIQENKEHRYSLGYFYDPPAGLYISPYCKPLSESPQIPLY 138
           VGD+LHIL+NG FPS+LHR  +   +  R+S+ Y + PP+ + ISP  K L +  Q PLY
Sbjct: 265 VGDLLHILTNGIFPSVLHRARVNHVRS-RFSMAYLWGPPSDIMISPLPK-LVDPLQSPLY 324

Query: 139 RCVNVQEYFTIKASNKGKGLSAIK 163
             +  ++Y   KA++  + LS I+
Sbjct: 325 PSLTWKQYLATKATHFNQSLSIIR 346

[Close]

BLAST of Bhi04G000830 vs. TAIR 10
Match: AT1G15550.1 (gibberellin 3-oxidase 1 )

HSP 1 Score: 142.9 bits (359), Expect = 2.3e-34
Identity = 70/145 (48.28%), Postives = 97/145 (66.90%), Query Frame = 0


Query: 18  ALRLNSYPPCPDPSRVVGLAAHTDTSLLTIIHQVRSDGLQVFNDGAGWVPLSPMSGTLIV 77
           AL+LN YP CP+P R +GLAAHTD++LLTI++Q  + GLQVF D  GWV + P  G+L+V
Sbjct: 211 ALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQVFRDDLGWVTVPPFPGSLVV 270

Query: 78  NVGDILHILSNGRFPSILHRVTIQENKEHRYSLGYFYDPPAGLYISPYCKPLSESPQIPL 137
           NVGD+ HILSNG F S+LHR  + + +  R S+ + + P + + ISP  K L    + PL
Sbjct: 271 NVGDLFHILSNGLFKSVLHRARVNQTRA-RLSVAFLWGPQSDIKISPVPK-LVSPVESPL 330

Query: 138 YRCVNVQEYFTIKASNKGKGLSAIK 163
           Y+ V  +EY   KA++  K LS I+
Sbjct: 331 YQSVTWKEYLRTKATHFNKALSMIR 353

[Close]

BLAST of Bhi04G000830 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 141.7 bits (356), Expect = 5.1e-34
Identity = 67/147 (45.58%), Postives = 97/147 (65.99%), Query Frame = 0


Query: 18  ALRLNSYPPCPDPSRVVGLAAHTDTSLLTIIHQVRSDGLQVFNDGAGWVPLSPMSGTLIV 77
           A+RLN YP CP+P R +GLAAHTD+++LTI+HQ  + GLQVF + +GWV + P  G L+V
Sbjct: 206 AIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPAPGVLVV 265

Query: 78  NVGDILHILSNGRFPSILHRVTIQENKEHRYSLGYFYDPPAG-LYISPYCKPLSESPQIP 137
           N+GD+ HILSNG+ PS++HR  +   +  R S+ Y +  PAG + I+P  K L+   +  
Sbjct: 266 NIGDLFHILSNGKIPSVVHRAKVNHTRS-RISIAYLWGGPAGDVQIAPISK-LTGPAEPS 325

Query: 138 LYRCVNVQEYFTIKASNKGKGLSAIKI 164
           LYR +  +EY  IK     K + AI++
Sbjct: 326 LYRSITWKEYLQIKYEVFDKAMDAIRV 350

[Close]

BLAST of Bhi04G000830 vs. TAIR 10
Match: AT4G21690.1 (gibberellin 3-oxidase 3 )

HSP 1 Score: 122.5 bits (306), Expect = 3.2e-28
Identity = 61/147 (41.50%), Postives = 91/147 (61.90%), Query Frame = 0


Query: 19  LRLNSYPPCPDPSRVVGLAAHTDTSLLTIIHQVRSDGLQVFN---DGAGWVPLSPMSGTL 78
           L+LNSYP CPDP   +GLA HTD+SLLTI++Q    GL++ +   +G+ W+ + P+ G+L
Sbjct: 206 LQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIESPQEEGSRWIGVEPIEGSL 265

Query: 79  IVNVGDILHILSNGRFPSILHRVTIQENKEHRYSLGYFYDPPAGLYISPYCKPLSESPQI 138
           +V +GD+ HI+SNG+F S +HR  + +   HR S  YF  PP  L I P     S+    
Sbjct: 266 VVIMGDLSHIISNGQFRSTMHRAVVNKT-HHRVSAAYFAGPPKNLQIGPL---TSDKNHP 325

Query: 139 PLYRCVNVQEYFTIKASNKGKGLSAIK 163
           P+YR +  +EY   KA++  K L+  +
Sbjct: 326 PIYRRLIWEEYLAAKATHFNKALTLFR 348

[Close]

BLAST of Bhi04G000830 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 110.2 bits (274), Expect = 1.6e-24
Identity = 58/149 (38.93%), Postives = 89/149 (59.73%), Query Frame = 0


Query: 16  YMALRLNSYPPCPDPSRVVGLAAHTDTSLLTIIHQVRS-DGLQVFNDGAGWVPLSPMSGT 75
           + ++++N YPPCP P +V+GL  H+D + LTI+ QV   +GLQ+  DG  WV + P+   
Sbjct: 208 WQSIKINYYPPCPQPDQVMGLTQHSDAAGLTILLQVNQVEGLQIKKDGK-WVVVKPLRDA 267

Query: 76  LIVNVGDILHILSNGRFPSILHRVTIQENKEHRYSLGYFYDPPAGLYISPYCKPLSESPQ 135
           L+VNVG+IL I++NGR+ SI HR  +   KE R S+  F+ P     I P  K L +  +
Sbjct: 268 LVVNVGEILEIITNGRYRSIEHRAVVNSEKE-RLSVAMFHSPGKETIIRP-AKSLVDRQK 327

Query: 136 IPLYRCVNVQEYFTIKASNKGKGLSAIKI 164
             L++ ++ QEYF    + K  G S + +
Sbjct: 328 QCLFKSMSTQEYFDAFFTQKLNGKSHLDL 353

[Close]

BLAST of Bhi04G000830 vs. ExPASy Swiss-Prot
Match: O24648 (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.5e-35
Identity = 70/150 (46.67%), Postives = 100/150 (66.67%), Query Frame = 0


Query: 14  KPYMALRLNSYPPCPDPSRVVGLAAHTDTSLLTIIHQVRSDGLQVFNDGAGWVPLSPMSG 73
           K   AL+LNSYP CPDP   +GLA HTD++ LTI+ Q    GLQV  +G+GW+ + P+ G
Sbjct: 206 KACAALQLNSYPSCPDPDHAMGLAPHTDSTFLTILSQNDISGLQVNREGSGWITVPPLQG 265

Query: 74  TLIVNVGDILHILSNGRFPSILHRVTIQENKEHRYSLGYFYDPPAGLYISPYCKPLSESP 133
            L+VNVGD+ HILSNG +PS+LHRV +   ++ R+S+ Y Y PP+ + I P+ K +  + 
Sbjct: 266 GLVVNVGDLFHILSNGLYPSVLHRVLVNRTRQ-RFSVAYLYGPPSNVEICPHAKLIGPT- 325

Query: 134 QIPLYRCVNVQEYFTIKASNKGKGLSAIKI 164
           + PLYR V   EY   KA +  K LS++++
Sbjct: 326 KPPLYRSVTWNEYLGTKAKHFNKALSSVRL 353

[Close]

BLAST of Bhi04G000830 vs. ExPASy Swiss-Prot
Match: Q9ZT84 (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 145.6 bits (366), Expect = 4.9e-34
Identity = 68/144 (47.22%), Postives = 99/144 (68.75%), Query Frame = 0


Query: 19  LRLNSYPPCPDPSRVVGLAAHTDTSLLTIIHQVRSDGLQVFNDGAGWVPLSPMSGTLIVN 78
           ++LN YP CP+P R +GLAAHTD++L+TI++Q  + GLQVF D  GWV   P+ G+L+VN
Sbjct: 205 IQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGWVTAPPVPGSLVVN 264

Query: 79  VGDILHILSNGRFPSILHRVTIQENKEHRYSLGYFYDPPAGLYISPYCKPLSESPQIPLY 138
           VGD+LHIL+NG FPS+LHR  +   +  R+S+ Y + PP+ + ISP  K L +  Q PLY
Sbjct: 265 VGDLLHILTNGIFPSVLHRARVNHVRS-RFSMAYLWGPPSDIMISPLPK-LVDPLQSPLY 324

Query: 139 RCVNVQEYFTIKASNKGKGLSAIK 163
             +  ++Y   KA++  + LS I+
Sbjct: 325 PSLTWKQYLATKATHFNQSLSIIR 346

[Close]

BLAST of Bhi04G000830 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 142.9 bits (359), Expect = 3.2e-33
Identity = 70/145 (48.28%), Postives = 97/145 (66.90%), Query Frame = 0


Query: 18  ALRLNSYPPCPDPSRVVGLAAHTDTSLLTIIHQVRSDGLQVFNDGAGWVPLSPMSGTLIV 77
           AL+LN YP CP+P R +GLAAHTD++LLTI++Q  + GLQVF D  GWV + P  G+L+V
Sbjct: 211 ALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQVFRDDLGWVTVPPFPGSLVV 270

Query: 78  NVGDILHILSNGRFPSILHRVTIQENKEHRYSLGYFYDPPAGLYISPYCKPLSESPQIPL 137
           NVGD+ HILSNG F S+LHR  + + +  R S+ + + P + + ISP  K L    + PL
Sbjct: 271 NVGDLFHILSNGLFKSVLHRARVNQTRA-RLSVAFLWGPQSDIKISPVPK-LVSPVESPL 330

Query: 138 YRCVNVQEYFTIKASNKGKGLSAIK 163
           Y+ V  +EY   KA++  K LS I+
Sbjct: 331 YQSVTWKEYLRTKATHFNKALSMIR 353

[Close]

BLAST of Bhi04G000830 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 7.1e-33
Identity = 67/147 (45.58%), Postives = 97/147 (65.99%), Query Frame = 0


Query: 18  ALRLNSYPPCPDPSRVVGLAAHTDTSLLTIIHQVRSDGLQVFNDGAGWVPLSPMSGTLIV 77
           A+RLN YP CP+P R +GLAAHTD+++LTI+HQ  + GLQVF + +GWV + P  G L+V
Sbjct: 206 AIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPAPGVLVV 265

Query: 78  NVGDILHILSNGRFPSILHRVTIQENKEHRYSLGYFYDPPAG-LYISPYCKPLSESPQIP 137
           N+GD+ HILSNG+ PS++HR  +   +  R S+ Y +  PAG + I+P  K L+   +  
Sbjct: 266 NIGDLFHILSNGKIPSVVHRAKVNHTRS-RISIAYLWGGPAGDVQIAPISK-LTGPAEPS 325

Query: 138 LYRCVNVQEYFTIKASNKGKGLSAIKI 164
           LYR +  +EY  IK     K + AI++
Sbjct: 326 LYRSITWKEYLQIKYEVFDKAMDAIRV 350

[Close]

BLAST of Bhi04G000830 vs. ExPASy Swiss-Prot
Match: Q9SVS8 (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 4.5e-27
Identity = 61/147 (41.50%), Postives = 91/147 (61.90%), Query Frame = 0


Query: 19  LRLNSYPPCPDPSRVVGLAAHTDTSLLTIIHQVRSDGLQVFN---DGAGWVPLSPMSGTL 78
           L+LNSYP CPDP   +GLA HTD+SLLTI++Q    GL++ +   +G+ W+ + P+ G+L
Sbjct: 206 LQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIESPQEEGSRWIGVEPIEGSL 265

Query: 79  IVNVGDILHILSNGRFPSILHRVTIQENKEHRYSLGYFYDPPAGLYISPYCKPLSESPQI 138
           +V +GD+ HI+SNG+F S +HR  + +   HR S  YF  PP  L I P     S+    
Sbjct: 266 VVIMGDLSHIISNGQFRSTMHRAVVNKT-HHRVSAAYFAGPPKNLQIGPL---TSDKNHP 325

Query: 139 PLYRCVNVQEYFTIKASNKGKGLSAIK 163
           P+YR +  +EY   KA++  K L+  +
Sbjct: 326 PIYRRLIWEEYLAAKATHFNKALTLFR 348

[Close]

BLAST of Bhi04G000830 vs. ExPASy TrEMBL
Match: Q9LM06 (Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus OX=3654 GN=WGA3H PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.6e-78
Identity = 141/163 (86.50%), Postives = 149/163 (91.41%), Query Frame = 0


Query: 1   MVKMLTSTCDSGGKPYMALRLNSYPPCPDPSRVVGLAAHTDTSLLTIIHQVRSDGLQVFN 60
           M+K+LTST +S GKPYMALRLNSYPPCPDPSRV+GLAAHTDTSL TI+HQ R DGLQVF 
Sbjct: 187 MMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHDGLQVFK 246

Query: 61  DGAGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVTIQENKEHRYSLGYFYDPPAGL 120
           DG GWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRV I ENKEHR+SL YFYDPP  L
Sbjct: 247 DGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFYDPPGDL 306

Query: 121 YISPYCKPLSESPQIPLYRCVNVQEYFTIKASNKGKGLSAIKI 164
           YISPYCKPLSESPQIPLYRCVNV+EYFTIKA  +GKGLSAIKI
Sbjct: 307 YISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 349

[Close]

BLAST of Bhi04G000830 vs. ExPASy TrEMBL
Match: A0A5A7VBH8 (Gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002890 PE=4 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 8.1e-72
Identity = 129/163 (79.14%), Postives = 144/163 (88.34%), Query Frame = 0


Query: 1   MVKMLTSTCDSGGKPYMALRLNSYPPCPDPSRVVGLAAHTDTSLLTIIHQVRSDGLQVFN 60
           M+K+LTST +S GKPYMALRLNSYPPCPDP +V+GLAAHTDTSL TI+HQVRS+GLQ+F 
Sbjct: 23  MMKLLTSTDESIGKPYMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVRSNGLQIFK 82

Query: 61  DGAGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVTIQENKEHRYSLGYFYDPPAGL 120
           DG GWVPLSPMSGTL+VNVGD+LHILSNGRF SILHRV IQENKEHR+SL YFYDPP  +
Sbjct: 83  DGTGWVPLSPMSGTLVVNVGDLLHILSNGRFTSILHRVMIQENKEHRFSLAYFYDPPGDI 142

Query: 121 YISPYCKPLSESPQIPLYRCVNVQEYFTIKASNKGKGLSAIKI 164
           YISPYCKPLS++PQ PLYR VNV+EYF IKA   G GL AIKI
Sbjct: 143 YISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKMGSGLPAIKI 185

[Close]

BLAST of Bhi04G000830 vs. ExPASy TrEMBL
Match: A0A1S3BBH9 (gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103488263 PE=3 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 8.1e-72
Identity = 129/163 (79.14%), Postives = 144/163 (88.34%), Query Frame = 0


Query: 1   MVKMLTSTCDSGGKPYMALRLNSYPPCPDPSRVVGLAAHTDTSLLTIIHQVRSDGLQVFN 60
           M+K+LTST +S GKPYMALRLNSYPPCPDP +V+GLAAHTDTSL TI+HQVRS+GLQ+F 
Sbjct: 189 MMKLLTSTDESIGKPYMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVRSNGLQIFK 248

Query: 61  DGAGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVTIQENKEHRYSLGYFYDPPAGL 120
           DG GWVPLSPMSGTL+VNVGD+LHILSNGRF SILHRV IQENKEHR+SL YFYDPP  +
Sbjct: 249 DGTGWVPLSPMSGTLVVNVGDLLHILSNGRFTSILHRVMIQENKEHRFSLAYFYDPPGDI 308

Query: 121 YISPYCKPLSESPQIPLYRCVNVQEYFTIKASNKGKGLSAIKI 164
           YISPYCKPLS++PQ PLYR VNV+EYF IKA   G GL AIKI
Sbjct: 309 YISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKMGSGLPAIKI 351

[Close]

BLAST of Bhi04G000830 vs. ExPASy TrEMBL
Match: S0BE47 (GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox4 PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.8e-71
Identity = 128/163 (78.53%), Postives = 145/163 (88.96%), Query Frame = 0


Query: 1   MVKMLTSTCDSGGKPYMALRLNSYPPCPDPSRVVGLAAHTDTSLLTIIHQVRSDGLQVFN 60
           M+K+LTST +S GKP+MALRLNSYPPCPDP +V+GLAAHTDTSL TI+HQV ++GLQ+F 
Sbjct: 188 MMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQIFK 247

Query: 61  DGAGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVTIQENKEHRYSLGYFYDPPAGL 120
           DG GWVPLSPMSGTLIVN+GD+LHILSNGRFPSILHRV IQENKEHR+SL YFYDPP  +
Sbjct: 248 DGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFYDPPGEI 307

Query: 121 YISPYCKPLSESPQIPLYRCVNVQEYFTIKASNKGKGLSAIKI 164
           YISPYCKPLS++PQ PLYR VNV+EYF IKA   GKGL AIKI
Sbjct: 308 YISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 350

[Close]

BLAST of Bhi04G000830 vs. ExPASy TrEMBL
Match: A0A0A0LSC5 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G379320 PE=4 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.8e-71
Identity = 128/163 (78.53%), Postives = 145/163 (88.96%), Query Frame = 0


Query: 1   MVKMLTSTCDSGGKPYMALRLNSYPPCPDPSRVVGLAAHTDTSLLTIIHQVRSDGLQVFN 60
           M+K+LTST +S GKP+MALRLNSYPPCPDP +V+GLAAHTDTSL TI+HQV ++GLQ+F 
Sbjct: 30  MMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGNNGLQIFK 89

Query: 61  DGAGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVTIQENKEHRYSLGYFYDPPAGL 120
           DG GWVPLSPMSGTLIVN+GD+LHILSNGRFPSILHRV IQENKEHR+SL YFYDPP  +
Sbjct: 90  DGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYFYDPPGEI 149

Query: 121 YISPYCKPLSESPQIPLYRCVNVQEYFTIKASNKGKGLSAIKI 164
           YISPYCKPLS++PQ PLYR VNV+EYF IKA   GKGL AIKI
Sbjct: 150 YISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 192

The following BLAST results are available for this feature:

BLAST of Bhi04G000830 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80340.1	3.5e-35	47.22	gibberellin 3-oxidase 2	[more]
AT1G15550.1	2.3e-34	48.28	gibberellin 3-oxidase 1	[more]
AT1G80330.1	5.1e-34	45.58	gibberellin 3-oxidase 4	[more]
AT4G21690.1	3.2e-28	41.50	gibberellin 3-oxidase 3	[more]
AT1G78550.1	1.6e-24	38.93	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

BLAST of Bhi04G000830 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24648	1.5e-35	46.67	Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1	[more]
Q9ZT84	4.9e-34	47.22	Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 ...	[more]
Q39103	3.2e-33	48.28	Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 ...	[more]
Q9C971	7.1e-33	45.58	Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ...	[more]
Q9SVS8	4.5e-27	41.50	Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 ...	[more]

BLAST of Bhi04G000830 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
Q9LM06	2.6e-78	86.50	Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus OX=3654 GN=WGA3H PE...	[more]
A0A5A7VBH8	8.1e-72	79.14	Gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A1S3BBH9	8.1e-72	79.14	gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103488263 PE...	[more]
S0BE47	1.8e-71	78.53	GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox4 PE=2 SV=1	[more]
A0A0A0LSC5	1.8e-71	78.53	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 39..60 score: 38.84 coord: 75..101 score: 31.99
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 15..154
None	No IPR available	PANTHER	PTHR47991:SF77	GIBBERELLIN 3-BETA-DIOXYGENASE 1-LIKE	coord: 15..154
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 7..155
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 3..161 e-value: 2.1E-41 score: 144.4
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 18..116 e-value: 2.2E-27 score: 95.5
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 14..116 score: 12.076986

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi04M000830	Bhi04M000830	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding