Bhi04G000781 (gene) Wax gourd (B227) v1

BLAST of Bhi04G000781 vs. TAIR 10
Match: AT3G11760.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G04860.1); Has 84 Blast hits to 73 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 84; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 299.7 bits (766), Expect = 5.2e-81
Identity = 226/700 (32.29%), Postives = 332/700 (47.43%), Query Frame = 0

Query: 5   KKFQVKLENLKLLGYG------KENERIAIEIKWKGPQRHSLLPVPFYAKSPLQTNRTTA 64
           +K++VKL   KL G+        E +R+ +EI+WKGP            K+ L + R + 
Sbjct: 17  RKYEVKLSVKKLEGWDLVREGVPEKDRLTVEIRWKGP------------KATLGSLRRSV 76

Query: 65  QRVLS----NHHQTVQW-NHEFHSMCEFEFPHDDASSIPFWDTKFYVLLEEEPTKSKTKS 124
           +R  +         V W + EF S+C     + D+   P W+  F V         K K+
Sbjct: 77  KRNFTKEAVGESDVVSWEDEEFQSLCSLT-SYKDSLFYP-WEITFSVFTNGMKQGQKNKA 136

Query: 125 SVLGKASLNLAEMLSAMETK-MERNVPIVLKDSAGAAPHHATISVCVNFVEVRDGPD--- 184
            V+G A LNLAE     + K  + N+P+ L  +  A+  H  + V ++ +E+R  P+   
Sbjct: 137 PVVGTAFLNLAEYACVTDKKEFDINIPLTL-SACVASETHPLLFVSLSLLELRTTPETSD 196

Query: 185 ------------------------PIQQEDKEGF---LKTLKDLTSF------KKKNREK 244
                                    +++ED       L+ +K  T F      KK  RE+
Sbjct: 197 SAAQTAVVPLPLPSPSPQQPTETHSVEKEDVSAIKAGLRKVKIFTEFVSTRKAKKACREE 256

Query: 245 GKVISSDGENRGLGDSTTGEEDG------------------------------------- 304
               SS   +  L D  T  ++G                                     
Sbjct: 257 EGRFSSFESSESLDDFETDFDEGKEELMSMRKSFSYGPLSYANGVGTSLNCGAKVSDEDE 316

Query: 305 -----DHKQG-------------------------KSLTKKRRLSFSFRHSRRKVEPWLE 364
                 H++                          +S+   R+   SFR  + K EP L+
Sbjct: 317 DWVYYSHRKSDVGAGCSDAEDSAAGLVYEASLLPRRSILPWRKRKLSFRSPKSKGEPLLK 376

Query: 365 KTN-TAVNDGVTVDRQR--HDNGASVFNVAPISTTSQMEKAESTEFSLETNIQYKEASGG 424
           K N     D +  DR++   D     F       +S   +   +EF  ++       + G
Sbjct: 377 KDNGEEGGDDIDFDRRQLSSDEAHPPFGSKIDEDSSANPRTSFSEFGEDS------FAIG 436

Query: 425 RWETREIVSRDGKLKLKTEVFFGSFDQRSEKAGGESACTAIVAVIAHWLHSNYGVMPTQL 484
            WE +E++SRDG +KL+T VF  S DQRSE+A GESACTA+VAVIA W   N  +MP + 
Sbjct: 437 SWEEKEVISRDGHMKLQTSVFLASIDQRSERAAGESACTALVAVIADWFQKNGNLMPIKS 496

Query: 485 ELDNLIMEGSSEWQKLCNNECYSNSFPNKHFDLETVVQADVGPITVSAENSFVGFFSPE- 544
           + D+LI EGS EW+ LC NE Y   FP+KHFDL+TV+QA + P+TV    SFVGFF P+ 
Sbjct: 497 QFDSLIREGSLEWRNLCENETYMQKFPDKHFDLDTVLQAKIRPLTVIPGKSFVGFFHPDG 556

Query: 545 -----KFNCLTEAMSFEQIWNEV-----NTKTFSNYES----RIYIVSWNDHFFVLKMDE 565
                +F  L  AMSF+ IW E+     ++    +Y+      +YIVSWNDHFFVLK+++
Sbjct: 557 MINEGRFEFLQGAMSFDSIWAEIISLEESSANGDSYDDDSPPHVYIVSWNDHFFVLKVEK 616

BLAST of Bhi04G000781 vs. TAIR 10
Match: AT5G04860.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G11760.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 284.3 bits (726), Expect = 2.3e-76
Identity = 232/691 (33.57%), Postives = 325/691 (47.03%), Query Frame = 0

Query: 24  ERIAIEIKWKGPQRHSLLPVPFYAKSPLQTNRTTAQRVLSNHHQTVQWNHEFHSMCEFEF 83
           +R  +EIKWKGP+  +L       K  +  N T       +    V+WN EF  +CEF  
Sbjct: 58  KRPVVEIKWKGPKSVTL-------KRSVVRNLTEEGGFRGD--GVVEWNEEFKRVCEFSV 117

Query: 84  PHDDASSIPFWDTKFYVLLEEEPTKSKTKSSVLGKASLNLAEMLSAM-ETKMERNVPIVL 143
            + + S +P++ +        + +K K +S   GKASLN+AE  S M E  ++  VP+  
Sbjct: 118 -YKEGSFLPWFVSLTVFSGLNQGSKEKVRS--FGKASLNIAEYFSLMKEDDVQVKVPLKD 177

Query: 144 KDSAGAAPHHATISVCVNFVE-----------VRDGPDPIQQEDKEGFLKT-LKDLTSF- 203
            DS+     H  IS+  +  E           V   P   + E  E  +K  L+ + +F 
Sbjct: 178 CDSSSVRSPHVHISLQFSPKESLPERQRSALPVLWSPLSAEAEKAESVVKVGLRKMKTFN 237

Query: 204 ----------KKKNREKGKVISSDGEN--------------------------------- 263
                     K+  ++      SDG++                                 
Sbjct: 238 NCMSSTQASEKESEKDGSSGSGSDGKSPERNLDSDSSYPFDTDSLDEGDAADESEENKEN 297

Query: 264 -------------------RGLGDSTTGEEDGD-----HK-------------------- 323
                              RG   + T  ED D     H+                    
Sbjct: 298 ESSLADPVNYKTLRSANWARGSFHTVTNPEDEDLIYYSHRSPLAETGHCSDEVSNDVVSL 357

Query: 324 -QGK-SLTKKRRLSF-----SFRHSRRKVEPWLEKTNTAVNDGVTVDRQRHDNGASVFNV 383
            Q K  ++KKR LS+     SFR  ++K EP L+K +     G  +D  R    +S    
Sbjct: 358 EQAKGQMSKKRMLSWKKRKLSFRSPKQKGEPLLKK-DCLEEGGDDIDFDRRQLSSS---- 417

Query: 384 APISTTSQMEKAESTEFSLETNIQYKEASGGRWETREIVSRDGKLKLKTEVFFGSFDQRS 443
               + S   +++       +     +   G WET+EI+SRDG +KL   VF  S DQRS
Sbjct: 418 --DESNSDWYRSDDAIMKPLSQFGDDDFVVGSWETKEIISRDGLMKLTARVFLASIDQRS 477

Query: 444 EKAGGESACTAIVAVIAHWLHSNYGVMPTQLELDNLIMEGSSEWQKLCNNECYSNSFPNK 503
           E+A GESACTA+VAV+AHWL SN  ++PT+ E D+LI EGSSEW+ +C NE Y   FP+K
Sbjct: 478 ERAAGESACTALVAVMAHWLGSNRDIIPTRSEFDSLIREGSSEWRNMCENEEYRERFPDK 537

Query: 504 HFDLETVVQADVGPITVSAENSFVGFFSPEK-----------FNCLTEAMSFEQIWNEV- 563
           HFDLETV+QA V PI V  E SF+GFF PEK            + L   MSF+ IW E+ 
Sbjct: 538 HFDLETVLQAKVRPICVVPERSFIGFFHPEKSEEEEGKEDASLDFLKGVMSFDSIWEELM 597

BLAST of Bhi04G000781 vs. TAIR 10
Match: AT2G25460.1 (CONTAINS InterPro DOMAIN/s: C2 calcium-dependent membrane targeting (InterPro:IPR000008); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G04860.1); Has 108 Blast hits to 69 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 108; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 215.7 bits (548), Expect = 9.9e-56
Identity = 162/470 (34.47%), Postives = 231/470 (49.15%), Query Frame = 0

Query: 3   KMKKFQVKLENLKLLGY--------GKENERIAIEIKWKGP-QRHSLLPVPFYAKSPLQT 62
           K++K  V ++ ++L G           +N    +E+KWKGP     L  VPFY +S    
Sbjct: 4   KVRKLHVTVKPVRLDGLPAILGDETAGKNLSAMVEVKWKGPVSGFGLGFVPFY-RSNRPV 63

Query: 63  NRTTAQRVL--SNHHQTVQWNHEFHSMCEFEFPHDDASSIPFWDTKFYVLLEEEPTKSKT 122
           N T+++ +   SNH   V+W  EF  +C    P         W+  F V   E    +K 
Sbjct: 64  NHTSSKPIALGSNH---VEWEEEFERVCCIVGP---------WNLSFNVFYGEN-MDAKN 123

Query: 123 KSSVLGKASLNLAEMLSAMETKMERNVPIVLKDSAGAAPHHATISVCVNFVEVRDGPDPI 182
           K S++GKASL+L+E+ S  E+ +ER +PI  K S       AT+ V V F EVR  PD  
Sbjct: 124 KKSLIGKASLDLSELASKQESTVERKLPIRSKGS--VLSKEATLVVNVTFSEVRTEPDDF 183

Query: 183 QQEDKEGFLKTLKDLTSFKKKNREKG----------KVISSDG------ENRGLGDSTTG 242
            Q    G +     +   K  +R  G             SS G      E          
Sbjct: 184 MQ---LGQISVDSAIPKKKSTSRRGGGSHFDSFSSPATASSSGGRSPVVETGLFSSPDEN 243

Query: 243 EEDGDHKQGKSLTKKRRLSFSFRHSRRKVEPWLEKTNTAVNDGVTVDRQRHDNGASVFNV 302
           + +   K G +  K+RRLSFS    R   E  + KT+T                      
Sbjct: 244 QSEPGQKAGFNWWKRRRLSFSMTWRREPREDEVTKTST---------------------- 303

Query: 303 APISTTSQMEKAESTEFSLETNIQYKEASGGRWETREIVSRDGKLKLKTEVFFGSFDQRS 362
                + ++EK  +TE  +E N         +W  +++VSRDGK KLK+EV+  S DQRS
Sbjct: 304 ---KPSEELEK-PATEIPIEAN---------KWVMKDLVSRDGKSKLKSEVYLASIDQRS 363

Query: 363 EKAGGESACTAIVAVIAHWLHSNYGVM-PTQLELDNLIMEGSSEWQKLCNNECYSNSFPN 422
           E+A GE+AC A+  V+AHW H+N  ++ P+    D+LI +GSS WQ LC+ E Y   FPN
Sbjct: 364 EQAAGEAACAAVAVVVAHWFHANPKLINPSGTAFDSLITQGSSLWQSLCDKESYLRLFPN 419

Query: 423 KHFDLETVVQADVGPITVSAENSFVGFFSPEKFNCLTEAMSFEQIWNEVN 445
           +HFDLET+V A++ P+ V  + SF G FSPE+F  L   MSF+QIW+E++
Sbjct: 424 RHFDLETIVSANLRPVRVCTDKSFTGLFSPERFASLDGLMSFDQIWDELS 419

BLAST of Bhi04G000781 vs. TAIR 10
Match: AT2G10560.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G04860.1); Has 70 Blast hits to 70 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 70; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 161.0 bits (406), Expect = 2.9e-39
Identity = 96/225 (42.67%), Postives = 130/225 (57.78%), Query Frame = 0

Query: 382 LCNNECYSNSFPNKHFDLETVVQADVGPITVSAENSFVGFFSPEK-----------FNCL 441
           +C NE Y   FP+KHFDLETV+QA V PI V  E +F+GFF  EK            + L
Sbjct: 1   MCENEEYRERFPDKHFDLETVLQAKVRPICVVPERTFIGFFHREKSKEEEEKEDVSLDFL 60

Query: 442 TEAMSFEQIWNEV--NTKTFSNYESRIYIVSWNDHFFVLKMDENACYIIDSLGERLFEGC 501
              MSF+ IW E+       S  E  IYIVSWNDH+FVL ++ +A YIID+LGER++EGC
Sbjct: 61  KGVMSFDSIWEEIMKQEPEESASEHVIYIVSWNDHYFVLLVNHDAYYIIDTLGERVYEGC 120

Query: 502 NQAYILKFDSS-------SLMFEN---------------------QEKGEVGE--LVCRG 561
           NQAY+LKFD         S++ +N                     +E  E GE  +VCRG
Sbjct: 121 NQAYVLKFDQDAEIKRLPSVIKDNKADMGSQKQGGKNKYEQPERSKESEEQGEEVVVCRG 180

Query: 562 KECCREFFERFLAAITIEELEEEQKKLGSCNFIPHQRLQIDFHFS 564
           KE CRE+ + FLAAI I++++ + K+    +F  H RLQI+ +++
Sbjct: 181 KESCREYIKSFLAAIPIQQVKADMKEGLVSSF--HHRLQIELYYT 223

BLAST of Bhi04G000781 vs. ExPASy TrEMBL
Match: A0A5A7VGR1 (C2 NT-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002390 PE=4 SV=1)

HSP 1 Score: 981.1 bits (2535), Expect = 2.0e-282
Identity = 500/587 (85.18%), Postives = 525/587 (89.44%), Query Frame = 0

Query: 1   MVKMKKFQVKLENLKLLGYGKENERIAIEIKWKGPQRHSLLPVPFYAKSPLQTNRTTAQR 60
           M KMKKFQVKL+NLKL GY KENERIAIEIKWKGPQRHSLL VPFYAKSPLQ NRT+AQ 
Sbjct: 1   MAKMKKFQVKLDNLKLYGYDKENERIAIEIKWKGPQRHSLLSVPFYAKSPLQINRTSAQP 60

Query: 61  VLSNHHQTVQWNHEFHSMCEFEFP-HDDASSIPFWDTKFYVLLEEEPTKSKTKSSVLGKA 120
           VL+NHH   QWNHEFHS+C FEFP H+D SSIPFWDTKFYVLLEE  TKSKTK+SVLGKA
Sbjct: 61  VLNNHH---QWNHEFHSICAFEFPHHEDPSSIPFWDTKFYVLLEES-TKSKTKTSVLGKA 120

Query: 121 SLNLAEMLSAMETKMERNVPIVLKDSAGAAPHHATISVCVNFVEVRDGPDPIQQEDKEGF 180
           SLNLAEML A+E KMERNVPI LKDSAGAAPHHA ISVCVNFVE+RDG D I Q+DKEGF
Sbjct: 121 SLNLAEMLLALERKMERNVPITLKDSAGAAPHHAMISVCVNFVEIRDGSDMIHQQDKEGF 180

Query: 181 LKTLKDLTSFKKKNREKGKVISSDGENRGLGDSTTGEEDGDHKQGKSLTKKRRLSFSFRH 240
           +K LK LTSFKKKNREKGK+ISSDGENRGLGD T GE+DGD K GK  +KKRRLSFSFRH
Sbjct: 181 MKALKGLTSFKKKNREKGKMISSDGENRGLGDPTIGEDDGDQKLGKLFSKKRRLSFSFRH 240

Query: 241 SRRKVEPWLEKTNTAVNDGVTVDRQRHDNGASVFNVAPISTTSQMEKAESTEFSLETNIQ 300
           S+ KVEPWLEKTNTAVNDGVTVD+Q+HDN  SV    PISTTSQMEK EST FSLET+ Q
Sbjct: 241 SKGKVEPWLEKTNTAVNDGVTVDQQQHDNDPSVLKDVPISTTSQMEKTESTAFSLETDGQ 300

Query: 301 YKEASGGRWETREIVSRDGKLKLKTEVFFGSFDQRSEKAGGESACTAIVAVIAHWLHSNY 360
            KE SGG+WETREIVSRDGK KLKTEVFFGSFDQRSEKAGGESACTAIVAVI HWLHSNY
Sbjct: 301 NKETSGGKWETREIVSRDGKTKLKTEVFFGSFDQRSEKAGGESACTAIVAVITHWLHSNY 360

Query: 361 GVMPTQLELDNLIMEGSSEWQKLCNNECYSNSFPNKHFDLETVVQADVGPITVSAENSFV 420
           G+MPTQ ELDNLIMEGSSEWQKLCNN CYSN FPNKHFDLETVVQADV PI VS E+SFV
Sbjct: 361 GMMPTQQELDNLIMEGSSEWQKLCNNVCYSNYFPNKHFDLETVVQADVRPIAVSPEDSFV 420

Query: 421 GFFSPEKFNCLTEAMSFEQIWNEVNTKTFSNYESRIYIVSWNDHFFVLKMDENACYIIDS 480
           GFFSPEKF+CLTEAMSFEQIWNEVN KT S YESRIYIVSWNDHFFV+KM+E+ACYIIDS
Sbjct: 421 GFFSPEKFSCLTEAMSFEQIWNEVNAKTCSIYESRIYIVSWNDHFFVMKMEEDACYIIDS 480

Query: 481 LGERLFEGCNQAYILKFDSSSLMFENQEKGEVGELVCRGKECCREFFERFLAAITIEELE 540
           LGERLFEGCNQAYILKFDSSSLMFENQEKGE GELVCRGKECCREFFERFLAAITIEELE
Sbjct: 481 LGERLFEGCNQAYILKFDSSSLMFENQEKGEAGELVCRGKECCREFFERFLAAITIEELE 540

Query: 541 EEQKKLGSCNFIPHQRLQIDFHFSSPVASSSSSSTSPSSLFFDQDSA 587
           EEQKKL   NF+PHQRLQIDFHFSSPVA  SSSSTSP SLF DQ  A
Sbjct: 541 EEQKKLSDFNFVPHQRLQIDFHFSSPVA--SSSSTSPYSLFSDQSPA 581

BLAST of Bhi04G000781 vs. ExPASy TrEMBL
Match: A0A1S3BBP9 (uncharacterized protein LOC103488316 OS=Cucumis melo OX=3656 GN=LOC103488316 PE=4 SV=1)

HSP 1 Score: 981.1 bits (2535), Expect = 2.0e-282
Identity = 500/587 (85.18%), Postives = 525/587 (89.44%), Query Frame = 0

Query: 1   MVKMKKFQVKLENLKLLGYGKENERIAIEIKWKGPQRHSLLPVPFYAKSPLQTNRTTAQR 60
           M KMKKFQVKL+NLKL GY KENERIAIEIKWKGPQRHSLL VPFYAKSPLQ NRT+AQ 
Sbjct: 1   MAKMKKFQVKLDNLKLYGYDKENERIAIEIKWKGPQRHSLLSVPFYAKSPLQINRTSAQP 60

Query: 61  VLSNHHQTVQWNHEFHSMCEFEFP-HDDASSIPFWDTKFYVLLEEEPTKSKTKSSVLGKA 120
           VL+NHH   QWNHEFHS+C FEFP H+D SSIPFWDTKFYVLLEE  TKSKTK+SVLGKA
Sbjct: 61  VLNNHH---QWNHEFHSICAFEFPHHEDPSSIPFWDTKFYVLLEES-TKSKTKTSVLGKA 120

Query: 121 SLNLAEMLSAMETKMERNVPIVLKDSAGAAPHHATISVCVNFVEVRDGPDPIQQEDKEGF 180
           SLNLAEML A+E KMERNVPI LKDSAGAAPHHA ISVCVNFVE+RDG D I Q+DKEGF
Sbjct: 121 SLNLAEMLLALERKMERNVPITLKDSAGAAPHHAMISVCVNFVEIRDGSDMIHQQDKEGF 180

Query: 181 LKTLKDLTSFKKKNREKGKVISSDGENRGLGDSTTGEEDGDHKQGKSLTKKRRLSFSFRH 240
           +K LK LTSFKKKNREKGK+ISSDGENRGLGD T GE+DGD K GK  +KKRRLSFSFRH
Sbjct: 181 MKALKGLTSFKKKNREKGKMISSDGENRGLGDPTIGEDDGDQKLGKLFSKKRRLSFSFRH 240

Query: 241 SRRKVEPWLEKTNTAVNDGVTVDRQRHDNGASVFNVAPISTTSQMEKAESTEFSLETNIQ 300
           S+ KVEPWLEKTNTAVNDGVTVD+Q+HDN  SV    PISTTSQMEK EST FSLET+ Q
Sbjct: 241 SKGKVEPWLEKTNTAVNDGVTVDQQQHDNDPSVLKDVPISTTSQMEKTESTAFSLETDGQ 300

Query: 301 YKEASGGRWETREIVSRDGKLKLKTEVFFGSFDQRSEKAGGESACTAIVAVIAHWLHSNY 360
            KE SGG+WETREIVSRDGK KLKTEVFFGSFDQRSEKAGGESACTAIVAVI HWLHSNY
Sbjct: 301 NKETSGGKWETREIVSRDGKTKLKTEVFFGSFDQRSEKAGGESACTAIVAVITHWLHSNY 360

Query: 361 GVMPTQLELDNLIMEGSSEWQKLCNNECYSNSFPNKHFDLETVVQADVGPITVSAENSFV 420
           G+MPTQ ELDNLIMEGSSEWQKLCNN CYSN FPNKHFDLETVVQADV PI VS E+SFV
Sbjct: 361 GMMPTQQELDNLIMEGSSEWQKLCNNVCYSNYFPNKHFDLETVVQADVRPIAVSPEDSFV 420

Query: 421 GFFSPEKFNCLTEAMSFEQIWNEVNTKTFSNYESRIYIVSWNDHFFVLKMDENACYIIDS 480
           GFFSPEKF+CLTEAMSFEQIWNEVN KT S YESRIYIVSWNDHFFV+KM+E+ACYIIDS
Sbjct: 421 GFFSPEKFSCLTEAMSFEQIWNEVNAKTCSIYESRIYIVSWNDHFFVMKMEEDACYIIDS 480

Query: 481 LGERLFEGCNQAYILKFDSSSLMFENQEKGEVGELVCRGKECCREFFERFLAAITIEELE 540
           LGERLFEGCNQAYILKFDSSSLMFENQEKGE GELVCRGKECCREFFERFLAAITIEELE
Sbjct: 481 LGERLFEGCNQAYILKFDSSSLMFENQEKGEAGELVCRGKECCREFFERFLAAITIEELE 540

Query: 541 EEQKKLGSCNFIPHQRLQIDFHFSSPVASSSSSSTSPSSLFFDQDSA 587
           EEQKKL   NF+PHQRLQIDFHFSSPVA  SSSSTSP SLF DQ  A
Sbjct: 541 EEQKKLSDFNFVPHQRLQIDFHFSSPVA--SSSSTSPYSLFSDQSPA 581

BLAST of Bhi04G000781 vs. ExPASy TrEMBL
Match: A0A0A0LM03 (C2 NT-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G381800 PE=4 SV=1)

HSP 1 Score: 971.5 bits (2510), Expect = 1.6e-279
Identity = 496/584 (84.93%), Postives = 520/584 (89.04%), Query Frame = 0

Query: 4   MKKFQVKLENLKLLGYGKENERIAIEIKWKGPQRHSLLPVPFYAKSPLQTNRTTAQRVLS 63
           MKKFQVKL+N KL GY KENERIAIEIKWKGPQRHSLL VPFYAKSPLQ NRTTAQ VL+
Sbjct: 1   MKKFQVKLDNFKLYGYNKENERIAIEIKWKGPQRHSLLSVPFYAKSPLQINRTTAQLVLN 60

Query: 64  NHHQTVQWNHEFHSMCEFEFP-HDDASSIPFWDTKFYVLLEEEPTKSKTKSSVLGKASLN 123
           NHH   QWNHEFHS+CEFE P HD  SSIPFWDTKFYVLLEE  TKSKTK+SVLGKASLN
Sbjct: 61  NHH---QWNHEFHSICEFELPHHDHPSSIPFWDTKFYVLLEEY-TKSKTKTSVLGKASLN 120

Query: 124 LAEMLSAMETKMERNVPIVLKDSAGAAPHHATISVCVNFVEVRDGPDPIQQEDKEGFLKT 183
           LAEML AMETKMERNVPI LKDSAGAAPHHA ISVCVNFVE+RDG D I Q+DKEGFLK 
Sbjct: 121 LAEMLLAMETKMERNVPITLKDSAGAAPHHAMISVCVNFVEIRDGSDMIHQQDKEGFLKA 180

Query: 184 LKDLTSFKKKNREKGKVISSDGENRGLGDSTTGEEDGDHKQGKSLTKKRRLSFSFRHSRR 243
           LK LTSFKKKNREKGKVISSDGENRGLGD T GEEDGD K GK L+KKRRLSFSFRHS+ 
Sbjct: 181 LKGLTSFKKKNREKGKVISSDGENRGLGDPTIGEEDGDQKLGKLLSKKRRLSFSFRHSKG 240

Query: 244 KVEPWLEKTNTAVNDGVTVDRQRHDNGASVFNVAPISTTSQMEKAESTEFSLETNIQYKE 303
           KVEPWLEKTNTAVNDGVTVD+  HDN  SV  V PIS TSQM+KAE+T FSLET+ Q KE
Sbjct: 241 KVEPWLEKTNTAVNDGVTVDQPEHDNDPSVLKVVPIS-TSQMDKAETTAFSLETDGQNKE 300

Query: 304 ASGGRWETREIVSRDGKLKLKTEVFFGSFDQRSEKAGGESACTAIVAVIAHWLHSNYGVM 363
            SGG+WETREI+SRDGK KLKTEVFFGSFDQRSEKAGGESACTAIVAVI HWLHSNYG M
Sbjct: 301 TSGGKWETREIISRDGKTKLKTEVFFGSFDQRSEKAGGESACTAIVAVITHWLHSNYGTM 360

Query: 364 PTQLELDNLIMEGSSEWQKLCNNECYSNSFPNKHFDLETVVQADVGPITVSAENSFVGFF 423
           PTQ ELD+LI+EGSSEWQKLCNN CYSN FPNKHFDLET+VQADV PI VSAENSFVGFF
Sbjct: 361 PTQPELDSLIIEGSSEWQKLCNNACYSNYFPNKHFDLETIVQADVRPIAVSAENSFVGFF 420

Query: 424 SPEKFNCLTEAMSFEQIWNEVNTKTFSNYESRIYIVSWNDHFFVLKMDENACYIIDSLGE 483
           SPEKFNCL EAMSFEQIWNEVN KT+S YESRIYIVSWNDHFFV+KM+E+ACYIIDSLGE
Sbjct: 421 SPEKFNCLAEAMSFEQIWNEVNAKTYSTYESRIYIVSWNDHFFVMKMEEDACYIIDSLGE 480

Query: 484 RLFEGCNQAYILKFDSSSLMFENQEKGEVGELVCRGKECCREFFERFLAAITIEELEEEQ 543
           RLFEGCNQAYILKFD SSLMF+NQEKGE GELVCRGKECCREFFERFLAAITIEELEEEQ
Sbjct: 481 RLFEGCNQAYILKFDKSSLMFKNQEKGEPGELVCRGKECCREFFERFLAAITIEELEEEQ 540

Query: 544 KKLGSCNFIPHQRLQIDFHFSSPVASSSSSSTSPSSLFFDQDSA 587
           KKL   NFIPHQRLQIDFHFSSPVASS S+  SP SLF D+  A
Sbjct: 541 KKLSDSNFIPHQRLQIDFHFSSPVASSPSN--SPCSLFSDRSPA 577

BLAST of Bhi04G000781 vs. ExPASy TrEMBL
Match: A0A6J1K753 (uncharacterized protein LOC111491769 OS=Cucurbita maxima OX=3661 GN=LOC111491769 PE=4 SV=1)

HSP 1 Score: 886.7 bits (2290), Expect = 5.2e-254
Identity = 464/585 (79.32%), Postives = 504/585 (86.15%), Query Frame = 0

Query: 1   MVKMKKFQVKLENLKLLGYGKENERIAIEIKWKGPQRHSLLPVPFYAKSPLQTNRTTAQR 60
           M KMKKFQVKL +LKL G+G E E IAIEIKWKGPQRHS+L VPFY KSPLQT+RTTAQR
Sbjct: 1   MAKMKKFQVKLGDLKLHGFGNEKEAIAIEIKWKGPQRHSILSVPFYGKSPLQTDRTTAQR 60

Query: 61  VLSNHHQTVQWNHEFHSMCEFEFPHDDASSIPFWDTKFYVLLEEEPTKSKTKSSVLGKAS 120
            L    QT+QW+HEF S+CEFEF  D  SS   WDTKFYVLLEE  TKSKTK SVLGKAS
Sbjct: 61  PLI---QTLQWDHEFLSICEFEFADD--SSTASWDTKFYVLLEES-TKSKTKFSVLGKAS 120

Query: 121 LNLAEMLSAMETKMERNVPIVLKDSAGAAPHHATISVCVNFVEVRDGPDPIQQEDKEGFL 180
           LNLA+ML  METK+ERNVPI+LKD    APH  T+SV VNFVEVRD  D IQQ+DKEGFL
Sbjct: 121 LNLAKMLLEMETKVERNVPIMLKD----APHQVTLSVRVNFVEVRDDSDTIQQQDKEGFL 180

Query: 181 KTLKDLTSFKKKNREKGKVISSDGENRGLGDSTTGEEDGDHKQ-GKSLTKKRRLSFSFRH 240
           K LKDLTSF+KKNR+KGK ISSDG+NR LGD    E+ GD KQ GK L+KKRRLSFSFR+
Sbjct: 181 KALKDLTSFRKKNRDKGKAISSDGQNRALGDPKE-EDGGDQKQLGKLLSKKRRLSFSFRY 240

Query: 241 SRRKVEPWLEKTNTAVNDGVTVDRQRHDNGASVFNVAPISTTSQMEKAESTEFSLETNIQ 300
           S+ KVEPW EKTNTAVNDGV+VDRQ+HD   +    APISTTSQ EK ESTE SLE + Q
Sbjct: 241 SKGKVEPWSEKTNTAVNDGVSVDRQQHDTEPT----APISTTSQTEKGESTEISLEIDEQ 300

Query: 301 YKEASGGRWETREIVSRDGKLKLKTEVFFGSFDQRSEKAGGESACTAIVAVIAHWLHSNY 360
            KE S GRWET+EIVSRDGK KLKTEVFFGSFDQRSEKAGGESACTAIVAVI HWLHSNY
Sbjct: 301 NKEDSRGRWETKEIVSRDGKAKLKTEVFFGSFDQRSEKAGGESACTAIVAVITHWLHSNY 360

Query: 361 GVMPTQLELDNLIMEGSSEWQKLCNNECYSNSFPNKHFDLETVVQADVGPITVSAENSFV 420
           G MPT+ ELDNLIMEGSSEWQKLCNN CYSNSFPNKHFDLETV++ADV PITVSAENSFV
Sbjct: 361 GAMPTRPELDNLIMEGSSEWQKLCNNHCYSNSFPNKHFDLETVMEADVRPITVSAENSFV 420

Query: 421 GFFSPEKFNCLTEAMSFEQIWNEVNTKTFSNYESRIYIVSWNDHFFVLKMDENACYIIDS 480
           GFFSPEKFNCL+EAMSFEQIWN VNTKT  NYE+R+YIVSWNDHFFVLKM+E+ACYI+DS
Sbjct: 421 GFFSPEKFNCLSEAMSFEQIWNLVNTKTCPNYETRVYIVSWNDHFFVLKMEEDACYIVDS 480

Query: 481 LGERLFEGCNQAYILKFDSSSLMFENQEKGEVGELVCRGKECCREFFERFLAAITIEELE 540
           LGERLFEGCNQAYILKFDSSSLM+E++E+GEVGELVC+GKECCREFFERFLAAITIEELE
Sbjct: 481 LGERLFEGCNQAYILKFDSSSLMYESKEEGEVGELVCKGKECCREFFERFLAAITIEELE 540

Query: 541 EEQKKLGSCNFIPHQRLQIDFHFSSPVASSSSSSTSPSSLFFDQD 585
           EEQKK  SC+FIPHQRLQIDFHFSSPV   SS STSP S+F DQD
Sbjct: 541 EEQKKQSSCSFIPHQRLQIDFHFSSPVV--SSLSTSPCSIFSDQD 568

BLAST of Bhi04G000781 vs. ExPASy TrEMBL
Match: A0A6J1HEA9 (uncharacterized protein LOC111462700 OS=Cucurbita moschata OX=3662 GN=LOC111462700 PE=4 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.3e-252
Identity = 462/587 (78.71%), Postives = 502/587 (85.52%), Query Frame = 0

Query: 1   MVKMKKFQVKLENLKLLGYGKENERIAIEIKWKGPQRHSLLPVPFYAKSPLQTNRTTAQR 60
           M KMKKFQVKL +LKL G+G E E IAIEIKWKGP RHS+L VPFY KSPLQT+RTTAQR
Sbjct: 1   MAKMKKFQVKLGDLKLHGFGNEKEAIAIEIKWKGPHRHSILSVPFYGKSPLQTDRTTAQR 60

Query: 61  VLSNHHQTVQWNHEFHSMCEFEFPHDDASSIPFWDTKFYVLLEEEPTKSKTKSSVLGKAS 120
            L    QT+QW+HEF S+CEFEF  DD+SSI  WDTKFYVLLEE  TKSKTK SVLGKAS
Sbjct: 61  ALIQTQQTLQWDHEFLSVCEFEFA-DDSSSIASWDTKFYVLLEES-TKSKTKFSVLGKAS 120

Query: 121 LNLAEMLSAMETKMERNVPIVLKDSAGAAPHHATISVCVNFVEVRDGPDPI-QQEDKEGF 180
           LNLAEML  MET +ERNVPI LK     APH AT+SV VNFVEVRD  DPI QQ+DKEGF
Sbjct: 121 LNLAEMLLEMETIVERNVPITLK----GAPHQATLSVRVNFVEVRDDSDPIQQQQDKEGF 180

Query: 181 LKTLKDLTSFKKKNREKGKVISSDGENRGLGDSTTGEEDGDHKQ-GKSLTKKRRLSFSFR 240
           LK LKDLTSF+KKNR+KGK +SSDG+NRG  D    E+ GD KQ GK L+KKRRLSFSFR
Sbjct: 181 LKALKDLTSFRKKNRDKGKAVSSDGQNRGFADPKE-EDGGDQKQLGKLLSKKRRLSFSFR 240

Query: 241 HSRRKVEPWLEKTNTAVNDGVTVDRQRHDNGASVFNVAPISTTSQMEKAESTEFSLETNI 300
           +S+ KVEPW EKTNTAVNDGV+VDRQ+HD   +    APIS TSQ EK ESTE SLE + 
Sbjct: 241 YSKGKVEPWSEKTNTAVNDGVSVDRQQHDTEPT----APISITSQTEKGESTEISLEIDE 300

Query: 301 QYKEASGGRWETREIVSRDGKLKLKTEVFFGSFDQRSEKAGGESACTAIVAVIAHWLHSN 360
           Q KE S GRWET+EIVSRDGK KLKTEVFFGSFDQRSEKAGGESACTAIVAVI HWLHSN
Sbjct: 301 QNKEDSRGRWETKEIVSRDGKAKLKTEVFFGSFDQRSEKAGGESACTAIVAVITHWLHSN 360

Query: 361 YGVMPTQLELDNLIMEGSSEWQKLCNNECYSNSFPNKHFDLETVVQADVGPITVSAENSF 420
           YG MPT+ ELDNLIMEGSSEWQKLCNN CYSNSFPNKHFDLETV++ADV PITVSAENSF
Sbjct: 361 YGAMPTRPELDNLIMEGSSEWQKLCNNHCYSNSFPNKHFDLETVMEADVRPITVSAENSF 420

Query: 421 VGFFSPEKFNCLTEAMSFEQIWNEVN-TKTFSNYESRIYIVSWNDHFFVLKMDENACYII 480
           VGFFSPEKFNCL+EAMSFEQIWNE+N TKT  NY +R+YIVSWNDHFFV KM+E+ACYI+
Sbjct: 421 VGFFSPEKFNCLSEAMSFEQIWNEINTTKTCPNYVTRVYIVSWNDHFFVFKMEEDACYIV 480

Query: 481 DSLGERLFEGCNQAYILKFDSSSLMFENQEKGEVGELVCRGKECCREFFERFLAAITIEE 540
           DSLGERLFEGCNQAYILKFDSSSLM+E++EKGEVGELVC+GKECCREFFERFLAAITIEE
Sbjct: 481 DSLGERLFEGCNQAYILKFDSSSLMYESKEKGEVGELVCKGKECCREFFERFLAAITIEE 540

Query: 541 LEEEQKKLGSCNFIPHQRLQIDFHFSSPVASSSSSSTSPSSLFFDQD 585
           LEEEQ+K  SCNFIPHQRLQIDFHFSSPV   SS STSP S+F DQD
Sbjct: 541 LEEEQEKQSSCNFIPHQRLQIDFHFSSPVV--SSLSTSPCSIFSDQD 574