Bhi04G000652 (gene) Wax gourd (B227) v1

Overview


Name	Bhi04G000652
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	RING-type E3 ubiquitin transferase
Location	chr4: 19524442 .. 19525949 (+)
RNA-Seq Expression	Bhi04G000652
Synteny	Bhi04G000652

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTTAAAATTTTGAATTAGTTTTTTTAAAATATGTTTTAAAATTAAGTAATAAGAGAAAATAAAAAATGATATTGAAATTTAACCTAATTGTTAAGGTGTGGAGCAAAGTCATTTTTATTTGTTTGTTTATTGTATTTCTTCAGACAAAAGAAATTGAGAGTGGAGCACGCTCATCTCCCTCTTTCTTCTTCCTTTTCCCATTTTTCACTCTGTTTCTCTCTCTCTGTCTCAGCATTCTGAGCTCTTCCAGAGCTCCCTCTGCCATGGCGGTTACAATTAGCAGAAAACTTTTCCCTGTTGCTACTGGAACTACCCAACCAACCACCAATTGTTCCGGCTGCCCTTACGACTGCCCTTTCGACTGCTACGGTATTCCAGATTTCTATTACTCGCAACCGCCGCCACCACCTCCTCTCGCCGATCACCAAAAACAGCTTCCAACTTTAATCATCATCCTGCTTCTGTTCGTTGGATTTTTCATCGCTTTTAGTTTCTACATCATCGTCGTAAAATGCCGATCTTGGCAAAGTGGCTCCGGCAACGAAGGGACAGAGGCGCTGCAATCGGACGGGGGGGAGGGAGAGTTTATGAACGAGAATCAAGTCGATCATCCAATTTGGTTCATCACCACCGCTGGACTTCAGCAATCAGTCATCAATTCGATTACGGTTTGCAAGTACAAGAAGAGCGAAGGTTTAATTGAAGGAACAGAGTGCTCTGTTTGTTTGAGTGAATTTCAAGAAGACGAAATGCTTCGGCTATTACCCAAATGCAGCCACGCCTTTCATATTGGGTGTGTTGATACTTGGCTAAGAACTCACACCACTTGCCCACTCTGTCGAGCCCATATTCTGACTGATTTCACGGCTTCCAACTCTGTGAGGCCACCAAACTTAGGTCCTGTGAATCAAAACGAAGCTAATTTGGGTGTAAATGAAGAAACCCAGATGGAGAATGAGGAAATTAATGGCGAAGTTTTAGTGGAAAATGAAGGGGTTGTTTCTCGTGGGAACTTTGAAAACAGAGGAGATGTTGTTGATGAACAGAGAGAAGTCGGTATTGTTCAGACTGATGATTCTAAAGTTGAAGGAAATGAAAGTACGAGGAACAATCAAATGGATTCTTCTTCAGCAACATGACATGAAAAAAAAGGAGATCAGATTGGTTTAATTCTTAGTATTGAAGAGGGTGAGCCTTGTATCAAAATTCTCTGCTCAATAGAGGGAAATTAATTGATAGCTTTTAATAGAAAGAGAGGGAGTGGCTATGATTTGATGGATTGCTTAAATAATCTATAAGTAATCTTTTGAACCCAGTTTTTGTCTGTGATTTGAAAATATAAGGAAATTGATAAAGGCGTTTGTTATTCATTGAATGGCTTTTTGTGGCCTCTGTTTTCTCTGATGATAATGGTTTCATGTTTGTTCTACATTGCCTTTCTGGGTATTATATTAAACTCTTTTATCTAAATAGAACAAAACAGAGCAAGGGGAGTTTCTTGTTTAA

mRNA sequence

CTTTTAAAATTTTGAATTAGTTTTTTTAAAATATGTTTTAAAATTAAGTAATAAGAGAAAATAAAAAATGATATTGAAATTTAACCTAATTGTTAAGGTGTGGAGCAAAGTCATTTTTATTTGTTTGTTTATTGTATTTCTTCAGACAAAAGAAATTGAGAGTGGAGCACGCTCATCTCCCTCTTTCTTCTTCCTTTTCCCATTTTTCACTCTGTTTCTCTCTCTCTGTCTCAGCATTCTGAGCTCTTCCAGAGCTCCCTCTGCCATGGCGGTTACAATTAGCAGAAAACTTTTCCCTGTTGCTACTGGAACTACCCAACCAACCACCAATTGTTCCGGCTGCCCTTACGACTGCCCTTTCGACTGCTACGGTATTCCAGATTTCTATTACTCGCAACCGCCGCCACCACCTCCTCTCGCCGATCACCAAAAACAGCTTCCAACTTTAATCATCATCCTGCTTCTGTTCGTTGGATTTTTCATCGCTTTTAGTTTCTACATCATCGTCGTAAAATGCCGATCTTGGCAAAGTGGCTCCGGCAACGAAGGGACAGAGGCGCTGCAATCGGACGGGGGGGAGGGAGAGTTTATGAACGAGAATCAAGTCGATCATCCAATTTGGTTCATCACCACCGCTGGACTTCAGCAATCAGTCATCAATTCGATTACGGTTTGCAAGTACAAGAAGAGCGAAGGTTTAATTGAAGGAACAGAGTGCTCTGTTTGTTTGAGTGAATTTCAAGAAGACGAAATGCTTCGGCTATTACCCAAATGCAGCCACGCCTTTCATATTGGGTGTGTTGATACTTGGCTAAGAACTCACACCACTTGCCCACTCTGTCGAGCCCATATTCTGACTGATTTCACGGCTTCCAACTCTGTGAGGCCACCAAACTTAGGTCCTGTGAATCAAAACGAAGCTAATTTGGGTGTAAATGAAGAAACCCAGATGGAGAATGAGGAAATTAATGGCGAAGTTTTAGTGGAAAATGAAGGGGTTGTTTCTCGTGGGAACTTTGAAAACAGAGGAGATGTTGTTGATGAACAGAGAGAAGTCGGTATTGTTCAGACTGATGATTCTAAAGTTGAAGGAAATGAAAGTACGAGGAACAATCAAATGGATTCTTCTTCAGCAACATGACATGAAAAAAAAGGAGATCAGATTGGTTTAATTCTTAGTATTGAAGAGGGTGAGCCTTGTATCAAAATTCTCTGCTCAATAGAGGGAAATTAATTGATAGCTTTTAATAGAAAGAGAGGGAGTGGCTATGATTTGATGGATTGCTTAAATAATCTATAAGTAATCTTTTGAACCCAGTTTTTGTCTGTGATTTGAAAATATAAGGAAATTGATAAAGGCGTTTGTTATTCATTGAATGGCTTTTTGTGGCCTCTGTTTTCTCTGATGATAATGGTTTCATGTTTGTTCTACATTGCCTTTCTGGGTATTATATTAAACTCTTTTATCTAAATAGAACAAAACAGAGCAAGGGGAGTTTCTTGTTTAA

Coding sequence (CDS)

ATGATATTGAAATTTAACCTAATTGTTAAGGTGTGGAGCAAAGTCATTTTTATTTGTTTGTTTATTGTATTTCTTCAGACAAAAGAAATTGAGAGTGGAGCACGCTCATCTCCCTCTTTCTTCTTCCTTTTCCCATTTTTCACTCTGTTTCTCTCTCTCTGTCTCAGCATTCTGAGCTCTTCCAGAGCTCCCTCTGCCATGGCGGTTACAATTAGCAGAAAACTTTTCCCTGTTGCTACTGGAACTACCCAACCAACCACCAATTGTTCCGGCTGCCCTTACGACTGCCCTTTCGACTGCTACGGTATTCCAGATTTCTATTACTCGCAACCGCCGCCACCACCTCCTCTCGCCGATCACCAAAAACAGCTTCCAACTTTAATCATCATCCTGCTTCTGTTCGTTGGATTTTTCATCGCTTTTAGTTTCTACATCATCGTCGTAAAATGCCGATCTTGGCAAAGTGGCTCCGGCAACGAAGGGACAGAGGCGCTGCAATCGGACGGGGGGGAGGGAGAGTTTATGAACGAGAATCAAGTCGATCATCCAATTTGGTTCATCACCACCGCTGGACTTCAGCAATCAGTCATCAATTCGATTACGGTTTGCAAGTACAAGAAGAGCGAAGGTTTAATTGAAGGAACAGAGTGCTCTGTTTGTTTGAGTGAATTTCAAGAAGACGAAATGCTTCGGCTATTACCCAAATGCAGCCACGCCTTTCATATTGGGTGTGTTGATACTTGGCTAAGAACTCACACCACTTGCCCACTCTGTCGAGCCCATATTCTGACTGATTTCACGGCTTCCAACTCTGTGAGGCCACCAAACTTAGGTCCTGTGAATCAAAACGAAGCTAATTTGGGTGTAAATGAAGAAACCCAGATGGAGAATGAGGAAATTAATGGCGAAGTTTTAGTGGAAAATGAAGGGGTTGTTTCTCGTGGGAACTTTGAAAACAGAGGAGATGTTGTTGATGAACAGAGAGAAGTCGGTATTGTTCAGACTGATGATTCTAAAGTTGAAGGAAATGAAAGTACGAGGAACAATCAAATGGATTCTTCTTCAGCAACATGA

Protein sequence

MILKFNLIVKVWSKVIFICLFIVFLQTKEIESGARSSPSFFFLFPFFTLFLSLCLSILSSSRAPSAMAVTISRKLFPVATGTTQPTTNCSGCPYDCPFDCYGIPDFYYSQPPPPPPLADHQKQLPTLIIILLLFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQVDHPIWFITTAGLQQSVINSITVCKYKKSEGLIEGTECSVCLSEFQEDEMLRLLPKCSHAFHIGCVDTWLRTHTTCPLCRAHILTDFTASNSVRPPNLGPVNQNEANLGVNEETQMENEEINGEVLVENEGVVSRGNFENRGDVVDEQREVGIVQTDDSKVEGNESTRNNQMDSSSAT

Homology

[Close]

BLAST of Bhi04G000652 vs. TAIR 10
Match: AT1G72220.1 (RING/U-box superfamily protein )

HSP 1 Score: 183.3 bits (464), Expect = 3.3e-46
Identity = 112/265 (42.26%), Postives = 139/265 (52.45%), Query Frame = 0


Query: 67  MAVTISRKLFPVATGTTQPTTNCSG--CPYDCPFDCYGIPDFYYSQPPPPP--------- 126
           MA    RKLFP     T  T +CS   C   CP++CY  PD+Y   P  PP         
Sbjct: 1   MARKKHRKLFPTLASETNKTLDCSNGVCDPICPYNCYPEPDYYTISPQLPPWSSSPQPSP 60

Query: 127 -----------PLADHQKQLPTLIIILLLFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEA 186
                      P  D    L  + II +      I  + + +V K  S      N+GT  
Sbjct: 61  CPSPSISAVYLPSQDSSSSLDAISIITITGAVLAILLTGFFLVAKFFSDSVNRVNQGTYQ 120

Query: 187 LQSDGG----EGEFMNENQVDHPIWFITTAGLQQSVINSITVCKYKKSEGLIEGTECSVC 246
             ++      E EF +  QVDHPIW I T GLQQS+INSIT+C YK+ +GLIE T+C VC
Sbjct: 121 SDNEDNDTVMEEEFQDREQVDHPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDCPVC 180

Query: 247 LSEFQEDEMLRLLPKCSHAFHIGCVDTWLRTHTTCPLCRAHILTDFTASNSVRPPNL-GP 305
           L+EF+EDE LRLLPKC+HAFHI C+DTWL +HT CPLCRA I     A  SV  P   GP
Sbjct: 181 LNEFEEDESLRLLPKCNHAFHISCIDTWLSSHTNCPLCRAGI-----AMISVTTPRYSGP 240

[Close]

BLAST of Bhi04G000652 vs. TAIR 10
Match: AT5G10380.1 (RING/U-box superfamily protein )

HSP 1 Score: 147.5 bits (371), Expect = 2.0e-35
Identity = 86/200 (43.00%), Postives = 114/200 (57.00%), Query Frame = 0


Query: 107 YYSQPPPPPPLADHQKQLPTLI-----IILLLFVGFFIAFSFYIIVVKCRSWQSGSGNEG 166
           Y   PPPPPP  +    LPT I     IIL LF+ FF+    Y+ + + R   + S   G
Sbjct: 25  YPPPPPPPPPPRELSLLLPTSICVVGSIILFLFLVFFL----YLHITQQRRISAASVTPG 84

Query: 167 TEALQSDGGEGEFMNENQVDHPIWFITTAGLQQSVINSITVCKYKKSEGLIEGTECSVCL 226
               Q D  E E  + +   H +W I T GL +S INSITV  +KK EG+I+GTECSVCL
Sbjct: 85  DTNQQEDEDETEERDFSDFHH-VWQIPTVGLHRSAINSITVVGFKKGEGIIDGTECSVCL 144

Query: 227 SEFQEDEMLRLLPKCSHAFHIGCVDTWLRTHTTCPLCRAHILTDFTASNSVRPPNLGPVN 286
           +EF+EDE LRLLPKCSHAFH+ C+DTWL +H  CPLCRA +L      +     N  P +
Sbjct: 145 NEFEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPVLLITEPPHQETETNHQPDS 204

Query: 287 QNEANLGVNEETQMENEEIN 302
           ++  +L   +++       N
Sbjct: 205 ESSNDLRGRQDSSRSRRNHN 219

[Close]

BLAST of Bhi04G000652 vs. TAIR 10
Match: AT4G33565.1 (RING/U-box superfamily protein )

HSP 1 Score: 132.9 bits (333), Expect = 5.2e-31
Identity = 89/234 (38.03%), Postives = 118/234 (50.43%), Query Frame = 0


Query: 92  CPYDCPFDCYGIPDFYYSQPPPPPPLADHQKQLPTLIII--------LLLFVGFFIAFSF 151
           C   C  +C  I +        PP    H   L  ++II        L L     + F F
Sbjct: 89  CSSYCLDECPSICEIVVISGLSPPAEIHHDDNLKRILIISASSIITTLFLLTLLVLCFKF 148

Query: 152 YI----IVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQVDHPIWFITTAGLQQSVINS 211
           Y      +   R W      E       DG          VDHPIW I T GL  +VI+S
Sbjct: 149 YYRRRRSITTSRRWSM----EEARNWDFDGPAPVI-----VDHPIWHIRTIGLNPTVISS 208

Query: 212 ITVCKYKKSEGLIEGTECSVCLSEFQEDEMLRLLPKCSHAFHIGCVDTWLRTHTTCPLCR 271
           I VC+Y K +G++EGT+CSVCLSEF+E+E LRLLPKC HAFH+ C+DTWLR+HT CPLCR
Sbjct: 209 IKVCQYSKKDGVVEGTDCSVCLSEFEEEETLRLLPKCKHAFHLYCIDTWLRSHTNCPLCR 268

Query: 272 AHILTDFTASNSVRPPNLGPVNQNEANLGVNEETQMENEEINGEVLVENEGVVS 314
           A I+    A+  +   + G     E ++ + EE   + +E   E+  E +G VS
Sbjct: 269 APIV---EANTMIDDHSEG---LEEISVMIPEENGDDTDE---EIEEERDGFVS 304

[Close]

BLAST of Bhi04G000652 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 7.0e-28
Identity = 100/303 (33.00%), Postives = 139/303 (45.87%), Query Frame = 0


Query: 77  PVATGTTQPT-TNCSG--CPYDCPFDCYGIPDFYYSQPPPPPPLADHQKQ------LPTL 136
           P   GTT  +  +CS   C   CP  CY I       PPPP    D +         P L
Sbjct: 7   PNPWGTTYDSYRDCSQGVCSVYCPQWCYVI------FPPPPSFYLDDEDDSSSSDFSPLL 66

Query: 137 IIILLLFVGFFIAFSFYIIVVK-CRSWQSGSGNEGTEALQSDGGEGEFM-----NENQVD 196
           I ++ +    FI  S+Y ++ K C   +  S +    A+     +  +      N N   
Sbjct: 67  IALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGAT 126

Query: 197 HPIWFITTA---GLQQSVINSITVCKYKKSEGLIEGTECSVCLSEFQEDEMLRLLPKCSH 256
           +P   I      GL +S+I SITV KY+K +G +E ++CSVCLSEFQE+E LRLLPKC+H
Sbjct: 127 NPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNH 186

Query: 257 AFHIGCVDTWLRTHTTCPLCRAHILTD--------------------FTASNSVRPPNLG 316
           AFH+ C+DTWL++H+ CPLCRA I+T                      T  +SV   NL 
Sbjct: 187 AFHVPCIDTWLKSHSNCPLCRAFIVTSSAVEIVDLTNQQIVTENNSISTGDDSVVVVNLD 246

Query: 317 PVNQNEANLGVNE---------ETQMENEEINGEVLVENEGVVSRGNFENRGDVVDEQRE 333
             N    N  VNE         +   + EE     L    GVVS  +     ++ D++  
Sbjct: 247 LENSRSRNETVNEGSTPKPPEMQDSRDGEERRSASLNSGGGVVSIADILR--EIEDDEES 301

[Close]

BLAST of Bhi04G000652 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 119.4 bits (298), Expect = 5.9e-27
Identity = 95/279 (34.05%), Postives = 140/279 (50.18%), Query Frame = 0


Query: 93  PYDCPFDC-YGIPDFYYSQ------PPPPPPL-------ADHQKQLPTLIIILLLFVGFF 152
           PYD   DC  GI + Y  Q      PPPPP         +      P LI ++ +     
Sbjct: 10  PYDSYNDCSQGICNIYCPQWCYLIFPPPPPSFFLDDDSSSSSSSFSPLLIALIGILTSAL 69

Query: 153 IAFSFYIIVVK-CRSWQSGSGNEGTEALQSDGGEGEFMNENQVDHPIWFITTAGLQQSVI 212
           I  S+Y ++ K C      S +E      +  GEG F +  ++          GL +S+I
Sbjct: 70  ILVSYYTLISKYCHRHHQTSSSETLNL--NHNGEGFFSSTQRIS-----TNGDGLNESMI 129

Query: 213 NSITVCKYKKSEGLIEGTECSVCLSEFQEDEMLRLLPKCSHAFHIGCVDTWLRTHTTCPL 272
            SITV KYK  +G ++G++CSVCLSEF+E+E LRLLPKC+HAFH+ C+DTWL++H+ CPL
Sbjct: 130 KSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNCPL 189

Query: 273 CRAHILTDFTASNSVRPPNLGPVNQNEANLGVNEETQMENEEINGEVLVENEGVVSR-GN 332
           CRA +         V  P    V QN + +  N+           E+ +   G  S+ G+
Sbjct: 190 CRAFV-------TGVNNPT-ASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQTGD 249

Query: 333 FENRGDVVDEQREVGIVQTD-DSKVEGNESTRNNQMDSS 355
           F++   VV E  E+G   +D  S+++  E  R  + + S
Sbjct: 250 FDS--VVVIEDLEIGSRNSDARSELQLPEERRETKDEDS 271

[Close]

BLAST of Bhi04G000652 vs. ExPASy Swiss-Prot
Match: Q8LFY8 (RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2)

HSP 1 Score: 183.3 bits (464), Expect = 4.7e-45
Identity = 112/265 (42.26%), Postives = 139/265 (52.45%), Query Frame = 0


Query: 67  MAVTISRKLFPVATGTTQPTTNCSG--CPYDCPFDCYGIPDFYYSQPPPPP--------- 126
           MA    RKLFP     T  T +CS   C   CP++CY  PD+Y   P  PP         
Sbjct: 1   MARKKHRKLFPTLASETNKTLDCSNGVCDPICPYNCYPEPDYYTISPQLPPWSSSPQPSP 60

Query: 127 -----------PLADHQKQLPTLIIILLLFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEA 186
                      P  D    L  + II +      I  + + +V K  S      N+GT  
Sbjct: 61  CPSPSISAVYLPSQDSSSSLDAISIITITGAVLAILLTGFFLVAKFFSDSVNRVNQGTYQ 120

Query: 187 LQSDGG----EGEFMNENQVDHPIWFITTAGLQQSVINSITVCKYKKSEGLIEGTECSVC 246
             ++      E EF +  QVDHPIW I T GLQQS+INSIT+C YK+ +GLIE T+C VC
Sbjct: 121 SDNEDNDTVMEEEFQDREQVDHPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDCPVC 180

Query: 247 LSEFQEDEMLRLLPKCSHAFHIGCVDTWLRTHTTCPLCRAHILTDFTASNSVRPPNL-GP 305
           L+EF+EDE LRLLPKC+HAFHI C+DTWL +HT CPLCRA I     A  SV  P   GP
Sbjct: 181 LNEFEEDESLRLLPKCNHAFHISCIDTWLSSHTNCPLCRAGI-----AMISVTTPRYSGP 240

[Close]

BLAST of Bhi04G000652 vs. ExPASy Swiss-Prot
Match: Q9LX93 (E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 2.9e-34
Identity = 86/200 (43.00%), Postives = 114/200 (57.00%), Query Frame = 0


Query: 107 YYSQPPPPPPLADHQKQLPTLI-----IILLLFVGFFIAFSFYIIVVKCRSWQSGSGNEG 166
           Y   PPPPPP  +    LPT I     IIL LF+ FF+    Y+ + + R   + S   G
Sbjct: 25  YPPPPPPPPPPRELSLLLPTSICVVGSIILFLFLVFFL----YLHITQQRRISAASVTPG 84

Query: 167 TEALQSDGGEGEFMNENQVDHPIWFITTAGLQQSVINSITVCKYKKSEGLIEGTECSVCL 226
               Q D  E E  + +   H +W I T GL +S INSITV  +KK EG+I+GTECSVCL
Sbjct: 85  DTNQQEDEDETEERDFSDFHH-VWQIPTVGLHRSAINSITVVGFKKGEGIIDGTECSVCL 144

Query: 227 SEFQEDEMLRLLPKCSHAFHIGCVDTWLRTHTTCPLCRAHILTDFTASNSVRPPNLGPVN 286
           +EF+EDE LRLLPKCSHAFH+ C+DTWL +H  CPLCRA +L      +     N  P +
Sbjct: 145 NEFEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPVLLITEPPHQETETNHQPDS 204

Query: 287 QNEANLGVNEETQMENEEIN 302
           ++  +L   +++       N
Sbjct: 205 ESSNDLRGRQDSSRSRRNHN 219

[Close]

BLAST of Bhi04G000652 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 127.1 bits (318), Expect = 4.0e-28
Identity = 79/214 (36.92%), Postives = 109/214 (50.93%), Query Frame = 0


Query: 84  QPTTNCSG--CPYDCPFDCYGIPDFYYSQPPPPP-----PLADHQKQ---LPTLIIILLL 143
           +PT +CS   C   CP  C      Y+  PPPPP       AD        P +I I+ +
Sbjct: 12  EPTRDCSQGLCSMYCPQWC------YFIFPPPPPFDVAGTSADDSSGPVFSPLVIAIIGV 71

Query: 144 FVGFFIAFSFYIIVVK-CRSWQS------GSGNEGTEALQSDGGEGEFMNENQVDHPIWF 203
               F+  S+Y  + K C +  S      GS + G       G  G   +     H  W 
Sbjct: 72  LASAFLLVSYYTFISKYCGTVSSLRGRVFGSSSGGAAYGGGAGSGGRHGHGQSRSHESWN 131

Query: 204 IT-TAGLQQSVINSITVCKYKKSEGLIEGTECSVCLSEFQEDEMLRLLPKCSHAFHIGCV 263
           ++  +GL +++IN ITVCKY++ +G +  T+CSVCL EF + E LRLLP+CSHAFH  C+
Sbjct: 132 VSPPSGLDETLINKITVCKYRRGDGFVHTTDCSVCLGEFSDGESLRLLPRCSHAFHQQCI 191

Query: 264 DTWLRTHTTCPLCRAHILTDFTASNSVRPPNLGP 280
           DTWL++H+ CPLCRA+I        S  P    P
Sbjct: 192 DTWLKSHSNCPLCRANITFVTVGLASPEPEGCAP 219

[Close]

BLAST of Bhi04G000652 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 122.5 bits (306), Expect = 9.8e-27
Identity = 100/303 (33.00%), Postives = 139/303 (45.87%), Query Frame = 0


Query: 77  PVATGTTQPT-TNCSG--CPYDCPFDCYGIPDFYYSQPPPPPPLADHQKQ------LPTL 136
           P   GTT  +  +CS   C   CP  CY I       PPPP    D +         P L
Sbjct: 7   PNPWGTTYDSYRDCSQGVCSVYCPQWCYVI------FPPPPSFYLDDEDDSSSSDFSPLL 66

Query: 137 IIILLLFVGFFIAFSFYIIVVK-CRSWQSGSGNEGTEALQSDGGEGEFM-----NENQVD 196
           I ++ +    FI  S+Y ++ K C   +  S +    A+     +  +      N N   
Sbjct: 67  IALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGAT 126

Query: 197 HPIWFITTA---GLQQSVINSITVCKYKKSEGLIEGTECSVCLSEFQEDEMLRLLPKCSH 256
           +P   I      GL +S+I SITV KY+K +G +E ++CSVCLSEFQE+E LRLLPKC+H
Sbjct: 127 NPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNH 186

Query: 257 AFHIGCVDTWLRTHTTCPLCRAHILTD--------------------FTASNSVRPPNLG 316
           AFH+ C+DTWL++H+ CPLCRA I+T                      T  +SV   NL 
Sbjct: 187 AFHVPCIDTWLKSHSNCPLCRAFIVTSSAVEIVDLTNQQIVTENNSISTGDDSVVVVNLD 246

Query: 317 PVNQNEANLGVNE---------ETQMENEEINGEVLVENEGVVSRGNFENRGDVVDEQRE 333
             N    N  VNE         +   + EE     L    GVVS  +     ++ D++  
Sbjct: 247 LENSRSRNETVNEGSTPKPPEMQDSRDGEERRSASLNSGGGVVSIADILR--EIEDDEES 301

[Close]

BLAST of Bhi04G000652 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 8.3e-26
Identity = 95/279 (34.05%), Postives = 140/279 (50.18%), Query Frame = 0


Query: 93  PYDCPFDC-YGIPDFYYSQ------PPPPPPL-------ADHQKQLPTLIIILLLFVGFF 152
           PYD   DC  GI + Y  Q      PPPPP         +      P LI ++ +     
Sbjct: 10  PYDSYNDCSQGICNIYCPQWCYLIFPPPPPSFFLDDDSSSSSSSFSPLLIALIGILTSAL 69

Query: 153 IAFSFYIIVVK-CRSWQSGSGNEGTEALQSDGGEGEFMNENQVDHPIWFITTAGLQQSVI 212
           I  S+Y ++ K C      S +E      +  GEG F +  ++          GL +S+I
Sbjct: 70  ILVSYYTLISKYCHRHHQTSSSETLNL--NHNGEGFFSSTQRIS-----TNGDGLNESMI 129

Query: 213 NSITVCKYKKSEGLIEGTECSVCLSEFQEDEMLRLLPKCSHAFHIGCVDTWLRTHTTCPL 272
            SITV KYK  +G ++G++CSVCLSEF+E+E LRLLPKC+HAFH+ C+DTWL++H+ CPL
Sbjct: 130 KSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNCPL 189

Query: 273 CRAHILTDFTASNSVRPPNLGPVNQNEANLGVNEETQMENEEINGEVLVENEGVVSR-GN 332
           CRA +         V  P    V QN + +  N+           E+ +   G  S+ G+
Sbjct: 190 CRAFV-------TGVNNPT-ASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQTGD 249

Query: 333 FENRGDVVDEQREVGIVQTD-DSKVEGNESTRNNQMDSS 355
           F++   VV E  E+G   +D  S+++  E  R  + + S
Sbjct: 250 FDS--VVVIEDLEIGSRNSDARSELQLPEERRETKDEDS 271

[Close]

BLAST of Bhi04G000652 vs. ExPASy TrEMBL
Match: A0A5A7VDK2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004620 PE=3 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 4.1e-137
Identity = 252/304 (82.89%), Postives = 269/304 (88.49%), Query Frame = 0


Query: 67  MAVTISRKLFPVATGTTQPTTNCSGCPYDCPFDCYGIPDFYYSQPPPPPPLADHQKQLPT 126
           MAVT SRKLFPVATGTTQPTTNCS CPYDCP+DCYG PD Y+S PPPPPPL DHQKQLPT
Sbjct: 1   MAVTHSRKLFPVATGTTQPTTNCSACPYDCPYDCYGFPDLYFSPPPPPPPLFDHQKQLPT 60

Query: 127 LIIILLLFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQVDHPIWF 186
           +II+L+LFV FFIAF+FYIIVVKCRSW SGSGNEG EALQSDGGEGEFMNENQVDHPIWF
Sbjct: 61  MIIVLILFVAFFIAFTFYIIVVKCRSWYSGSGNEGAEALQSDGGEGEFMNENQVDHPIWF 120

Query: 187 ITTAGLQQSVINSITVCKYKKSEGLIEGTECSVCLSEFQEDEMLRLLPKCSHAFHIGCVD 246
           ITTAGLQQSVINSITVCKYKKSEGLIEGT+CSVCLSEFQEDEMLRLLPKCSHAFHIGCVD
Sbjct: 121 ITTAGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGCVD 180

Query: 247 TWLRTHTTCPLCRAHILTDFTA--SNSVRPPNLGPVNQNEANLGVNEETQMENEEINGEV 306
           TWLRTHTTCPLCRAHILTDFT   SNS+RPPNLGP+NQNE NLG+NE+TQMENE INGE 
Sbjct: 181 TWLRTHTTCPLCRAHILTDFTTSNSNSIRPPNLGPLNQNEGNLGLNEDTQMENENINGEA 240

Query: 307 LVENEG---VVSRGNFENRGDVVDEQREV-------GIVQTDDSKVEGNESTRN-NQMDS 358
           + ENEG    +S  + ENRGDVVDEQREV       G  ++DDSKVEGNESTRN N+M S
Sbjct: 241 VRENEGGVVSISSESSENRGDVVDEQREVEEIVQKEGNFESDDSKVEGNESTRNDNRMYS 300

[Close]

BLAST of Bhi04G000652 vs. ExPASy TrEMBL
Match: A0A1S3BAW8 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103488086 PE=3 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 4.1e-137
Identity = 252/304 (82.89%), Postives = 269/304 (88.49%), Query Frame = 0


Query: 67  MAVTISRKLFPVATGTTQPTTNCSGCPYDCPFDCYGIPDFYYSQPPPPPPLADHQKQLPT 126
           MAVT SRKLFPVATGTTQPTTNCS CPYDCP+DCYG PD Y+S PPPPPPL DHQKQLPT
Sbjct: 1   MAVTHSRKLFPVATGTTQPTTNCSACPYDCPYDCYGFPDLYFSPPPPPPPLFDHQKQLPT 60

Query: 127 LIIILLLFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQVDHPIWF 186
           +II+L+LFV FFIAF+FYIIVVKCRSW SGSGNEG EALQSDGGEGEFMNENQVDHPIWF
Sbjct: 61  MIIVLILFVAFFIAFTFYIIVVKCRSWYSGSGNEGAEALQSDGGEGEFMNENQVDHPIWF 120

Query: 187 ITTAGLQQSVINSITVCKYKKSEGLIEGTECSVCLSEFQEDEMLRLLPKCSHAFHIGCVD 246
           ITTAGLQQSVINSITVCKYKKSEGLIEGT+CSVCLSEFQEDEMLRLLPKCSHAFHIGCVD
Sbjct: 121 ITTAGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGCVD 180

Query: 247 TWLRTHTTCPLCRAHILTDFTA--SNSVRPPNLGPVNQNEANLGVNEETQMENEEINGEV 306
           TWLRTHTTCPLCRAHILTDFT   SNS+RPPNLGP+NQNE NLG+NE+TQMENE INGE 
Sbjct: 181 TWLRTHTTCPLCRAHILTDFTTSNSNSIRPPNLGPLNQNEGNLGLNEDTQMENENINGEA 240

Query: 307 LVENEG---VVSRGNFENRGDVVDEQREV-------GIVQTDDSKVEGNESTRN-NQMDS 358
           + ENEG    +S  + ENRGDVVDEQREV       G  ++DDSKVEGNESTRN N+M S
Sbjct: 241 VRENEGGVVSISSESSENRGDVVDEQREVEEIVQKEGNFESDDSKVEGNESTRNDNRMYS 300

[Close]

BLAST of Bhi04G000652 vs. ExPASy TrEMBL
Match: A0A0A0LPJ4 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G369190 PE=3 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.5e-134
Identity = 244/302 (80.79%), Postives = 263/302 (87.09%), Query Frame = 0


Query: 67  MAVTISRKLFPVATGTTQPTTNCSGCPYDCPFDCYGIPDFYYSQPPPPPPLADHQKQLPT 126
           MAVT+SRKLFPVATGT  PTTNCS CPYDCP+DCYG PD Y+S PPPPPPL+DHQKQLPT
Sbjct: 1   MAVTLSRKLFPVATGTALPTTNCSACPYDCPYDCYGFPDLYFSPPPPPPPLSDHQKQLPT 60

Query: 127 LIIILLLFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQVDHPIWF 186
            IIIL+LF+ FF+AF+FYIIVVKCRSW SGS NEG EALQSDGGEGEFMNENQVDHPIWF
Sbjct: 61  TIIILILFIAFFVAFTFYIIVVKCRSWYSGSANEGAEALQSDGGEGEFMNENQVDHPIWF 120

Query: 187 ITTAGLQQSVINSITVCKYKKSEGLIEGTECSVCLSEFQEDEMLRLLPKCSHAFHIGCVD 246
           ITTAGLQQSVINSITVCKYKKSEGLIEGT+CSVCLSEFQEDEMLRLLPKCSHAFHIGCVD
Sbjct: 121 ITTAGLQQSVINSITVCKYKKSEGLIEGTDCSVCLSEFQEDEMLRLLPKCSHAFHIGCVD 180

Query: 247 TWLRTHTTCPLCRAHILTDFTASNSVRPPNLGPVNQNEANLGVNEETQMENEEINGEVLV 306
           TWLRTHTTCPLCRAHILTDFT  NSVRPPN+GP+NQNE NLG+NE+TQMENE  N E + 
Sbjct: 181 TWLRTHTTCPLCRAHILTDFTTPNSVRPPNIGPLNQNEGNLGLNEDTQMENENTNREAVR 240

Query: 307 ENEG---VVSRGNFENRGDVVDEQREV-------GIVQTDDSKVEGNESTRN-NQMDSSS 358
           ENEG    +S  + ENRGD VDEQREV       G  ++DDSKVEGNES RN NQM+SSS
Sbjct: 241 ENEGGGVSISSESSENRGDAVDEQREVEEIVQKEGNFESDDSKVEGNESMRNDNQMNSSS 300

[Close]

BLAST of Bhi04G000652 vs. ExPASy TrEMBL
Match: A0A6J1K4G1 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492196 PE=3 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 1.1e-110
Identity = 214/304 (70.39%), Postives = 238/304 (78.29%), Query Frame = 0


Query: 67  MAVTISRKLFPVATGTTQPTTNCSGCPYDCPFDCYGIPDFYYSQPPPPPPLADHQKQLPT 126
           MAV I+RKLFPV TGT Q + NCSGCPYDCPFDC  +PDFY+S   PPPP  DHQKQLPT
Sbjct: 1   MAVGITRKLFPVETGTHQQSFNCSGCPYDCPFDCLDLPDFYFS---PPPPFGDHQKQLPT 60

Query: 127 LIIILL-LFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQVDHPIW 186
           ++I++L LFVGFF  FSFYII+VK ++W+SGSGN+G EA Q DG +GEF+NENQVDHPIW
Sbjct: 61  IVIVILALFVGFFFTFSFYIIIVKWQAWRSGSGNQGAEASQLDGEDGEFLNENQVDHPIW 120

Query: 187 FITTAGLQQSVINSITVCKYKKSEGLIEGTECSVCLSEFQEDEMLRLLPKCSHAFHIGCV 246
           FITT GLQQS+INSITVCKYKK EGLIEGT CSVCL+EFQEDEMLRLLPKCSHAFHIGCV
Sbjct: 121 FITTVGLQQSIINSITVCKYKKGEGLIEGTVCSVCLNEFQEDEMLRLLPKCSHAFHIGCV 180

Query: 247 DTWLRTHTTCPLCRAHILTDFTASNSVRPPNLGPVNQNEANLGVNEETQMENEEINGEVL 306
           DTWLRTHTTCPLCRAHILTDF  SN  RPPN G VNQNEANL V EETQMEN  + GE  
Sbjct: 181 DTWLRTHTTCPLCRAHILTDFATSNFPRPPNSGHVNQNEANLAVIEETQMEN--LEGE-- 240

Query: 307 VENEGVVSRGNFENRGDVVDEQREVGIVQ------------TDDSKVEGNESTRNNQMDS 358
            E   V   G  ENRGD VDEQR+VG+ Q            +DDSK+E NE+     M+ 
Sbjct: 241 -EQSAVSGGGGVENRGDAVDEQRDVGVPQIGEIVPKEGVFESDDSKMEVNET----MMNC 292

[Close]

BLAST of Bhi04G000652 vs. ExPASy TrEMBL
Match: A0A6J1HEB6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111462708 PE=3 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 7.4e-110
Identity = 211/306 (68.95%), Postives = 239/306 (78.10%), Query Frame = 0


Query: 67  MAVTISRKLFPVATGTTQPTTNCSGCPYDCPFDCYGIPDFYYSQPP--PPPPLADHQKQL 126
           MAV I+RKLFPVA GT Q + NCS CPYDCPFDC  +PDFY+S PP  P PP  DHQKQL
Sbjct: 1   MAVGITRKLFPVAAGTHQQSFNCSACPYDCPFDCLDLPDFYFSPPPPLPLPPFGDHQKQL 60

Query: 127 PTLIIILL-LFVGFFIAFSFYIIVVKCRSWQSGSGNEGTEALQSDGGEGEFMNENQVDHP 186
           PT++I++L LFVGFF  FSFYII+VKC++W+SGSGN+G EA Q +G +GEF+NENQVDHP
Sbjct: 61  PTIVIVILALFVGFFFTFSFYIIIVKCQAWRSGSGNQGAEASQLNGEDGEFLNENQVDHP 120

Query: 187 IWFITTAGLQQSVINSITVCKYKKSEGLIEGTECSVCLSEFQEDEMLRLLPKCSHAFHIG 246
           IWFITT GLQQS+INSITVCKYKK EGLIEGT CSVCL+EFQEDEMLRLLPKCSHAFHIG
Sbjct: 121 IWFITTVGLQQSIINSITVCKYKKGEGLIEGTVCSVCLNEFQEDEMLRLLPKCSHAFHIG 180

Query: 247 CVDTWLRTHTTCPLCRAHILTDFTASNSVRPPNLGPVNQNEANLGVNEETQMENEEINGE 306
           CVDTWLRTHTTCPLCRAHILTDF  SN+ RPPN G +NQNEANLGV EETQMEN  + GE
Sbjct: 181 CVDTWLRTHTTCPLCRAHILTDFPTSNAARPPNSGHLNQNEANLGVIEETQMEN--LEGE 240

Query: 307 VLVENEGVVSRGNFENRGDVVDEQREVGIVQ------------TDDSKVEGNESTRNNQM 358
              E   V   G  ENRGD V EQR++G+ Q            +DDSK+E  E+     M
Sbjct: 241 ---EQSAVSGGGGVENRGDAVYEQRDIGVPQIGEIVPKEGVFESDDSKMEVTET----MM 297

The following BLAST results are available for this feature:

BLAST of Bhi04G000652 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G72220.1	3.3e-46	42.26	RING/U-box superfamily protein	[more]
AT5G10380.1	2.0e-35	43.00	RING/U-box superfamily protein	[more]
AT4G33565.1	5.2e-31	38.03	RING/U-box superfamily protein	[more]
AT3G03550.1	7.0e-28	33.00	RING/U-box superfamily protein	[more]
AT5G17600.1	5.9e-27	34.05	RING/U-box superfamily protein	[more]

BLAST of Bhi04G000652 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LFY8	4.7e-45	42.26	RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2	[more]
Q9LX93	2.9e-34	43.00	E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 ...	[more]
Q7XLY8	4.0e-28	36.92	E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994...	[more]
Q9SRQ8	9.8e-27	33.00	RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2	[more]
Q9LF64	8.3e-26	34.05	RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1	[more]

BLAST of Bhi04G000652 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VDK2	4.1e-137	82.89	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A1S3BAW8	4.1e-137	82.89	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103488086 PE=3 ...	[more]
A0A0A0LPJ4	1.5e-134	80.79	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G369190 PE...	[more]
A0A6J1K4G1	1.1e-110	70.39	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492196 P...	[more]
A0A6J1HEB6	7.4e-110	68.95	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111462708...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM01197	FANCL_C_2	coord: 213..267 e-value: 0.0079 score: -0.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 319..343
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 317..357
None	No IPR available	PANTHER	PTHR45676:SF121	TRANSCRIPTION FACTOR C2H2 FAMILY-RELATED	coord: 73..350
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 216..259 e-value: 1.19219E-18 score: 76.5717
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 207..265
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 217..258 e-value: 1.1E-6 score: 38.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 216..259 e-value: 1.7E-13 score: 50.6
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 217..259 score: 12.740079
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 191..266 e-value: 1.2E-21 score: 77.7
IPR044675	E3 ubiquitin-protein ligase RING1-like	PANTHER	PTHR45676	RING-H2 FINGER PROTEIN ATL51-RELATED	coord: 73..350

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi04M000652	Bhi04M000652	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane