Bhi04G000459 (gene) Wax gourd (B227) v1

BLAST of Bhi04G000459 vs. TAIR 10
Match: AT1G55120.1 (beta-fructofuranosidase 5 )

HSP 1 Score: 627.5 bits (1617), Expect = 1.1e-179
Identity = 303/544 (55.70%), Postives = 389/544 (71.51%), Query Frame = 0

Query: 63  QIQRTSYHFQPLKNWMNDPNGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWV 122
           Q  RT YHFQPLKNWMNDPNGPM YKGIYHLFYQ+NP GAV++ ++VW HS S DL+NW+
Sbjct: 36  QPYRTGYHFQPLKNWMNDPNGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWI 95

Query: 123 HLNHALEPSDPFDINGCWSGSVSFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEK 182
               A  PS P DINGCWSGSV+ L   KP+ILYTGID +  QVQN+A+P+N SDP+L +
Sbjct: 96  SQPPAFNPSQPSDINGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLRE 155

Query: 183 WRKSSQNPIIA--PPNGVERDKFRDPTTAWQGPDGEWRVAVGGHT-SYGGAAMLYRSEDF 242
           W K  QNP++     NG+  D+FRDPTTAW G DGEWRV VG  T    G A+LY+S DF
Sbjct: 156 WSKPPQNPLMTTNAVNGINPDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDF 215

Query: 243 VHWHQFRFPLYSSRDSGTWECPDFYPVMLNGTNGIDFSSGFGFGVKYVMKASF--NSHDY 302
            +W Q   PL+    +G WECPDF+PV + G++G++ SS    G+K+V+K S     HDY
Sbjct: 216 FNWTQSMKPLHYEDLTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDY 275

Query: 303 YTLGSYATEKEKFTSDSSSGFDFRGTNLGLRYDYGKFYASKTFYDSSKKRRILWGWVNES 362
           YT+GSY  EK+ +  D      F       R DYGK+YASKTFYD  KKRRILWGWVNES
Sbjct: 276 YTIGSYDREKDVYVPD----LGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNES 335

Query: 363 DSREDDIKKGWSGLQAVPRKIWLSKTGRQLTQWPVKEIEMLRRNHFSLHHKELRGRSTME 422
              +DDI+KGWSGLQ+ PRKIWL ++G++L QWP++EIE LR    +   K L+  ST++
Sbjct: 336 SPAKDDIEKGWSGLQSFPRKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQ 395

Query: 423 ILGGSASQVDVEVSFESPNLEEAESVETRWDDLDPQLLCSKRDASVNGRIGPFGLLVLAS 482
           + G +A+Q DVEVSF+   LE+A+ +E  W   DPQ +CS+ D SV   +GPFGL+VLAS
Sbjct: 396 VHGVTAAQADVEVSFKVKELEKADVIEPSW--TDPQKICSQGDLSVMSGLGPFGLMVLAS 455

Query: 483 NDLSEHTAIFFHILKAHN------RYVVLMCSDQSRSSFRKGVDKTTYGAFVDVDPCHKK 542
           ND+ E+T+++F I K+++      +YVVLMCSDQSRSS     DK+T+GAFV +DP H+ 
Sbjct: 456 NDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQT 515

Query: 543 ISLRTLVDHTIVESFGGKGKTCITSRVYPTLAVANDARLYAFNNGTQTVVISSLEVWNMS 596
           ISLRTL+DH+IVES+GG G+TCITSRVYP LA+  +A L+ FN GTQ+V I +L  W++ 
Sbjct: 516 ISLRTLIDHSIVESYGGGGRTCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLK 573

BLAST of Bhi04G000459 vs. TAIR 10
Match: AT3G13790.1 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 615.9 bits (1587), Expect = 3.4e-176
Identity = 306/546 (56.04%), Postives = 392/546 (71.79%), Query Frame = 0

Query: 63  QIQRTSYHFQPLKNWMNDPNGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWV 122
           Q  RT +HFQP KNWMNDPNGPM YKGIYHLFYQ NP GAV+ + +VWAHS S DLINW 
Sbjct: 49  QPYRTGFHFQPPKNWMNDPNGPMIYKGIYHLFYQWNPKGAVWGN-IVWAHSTSTDLINWD 108

Query: 123 HLNHALEPSDPFDINGCWSGSVSFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEK 182
               A+ PS PFDINGCWSGS + L   KP+ILYTGID   QQVQN+A P N SDP+L +
Sbjct: 109 PHPPAIFPSAPFDINGCWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLRE 168

Query: 183 WRKSSQNPIIAPP--NGVERDKFRDPTTAWQGPDGEWRVAVGGHTSYGGAAMLYRSEDFV 242
           W+KS  NP++AP   NG+    FRDPTTAW G D +WRV +G      G A+ Y S+DF+
Sbjct: 169 WKKSPLNPLMAPDAVNGINASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFL 228

Query: 243 HWHQFRFPLYSSRDSGTWECPDFYPVMLNGTNGIDFSSGFGFG-----VKYVMKASFN-- 302
            W +   PL+    SG WECPDF+PV   G+NG++ SS   FG     +K+V+K S +  
Sbjct: 229 KWEKSPEPLHYDDGSGMWECPDFFPVTRFGSNGVETSS---FGEPNEILKHVLKISLDDT 288

Query: 303 SHDYYTLGSYATEKEKFTSDSSSGFDFRGTNLGLRYDYGKFYASKTFYDSSKKRRILWGW 362
            HDYYT+G+Y   K+KF  D  +GF   GT    RYDYGK+YASKTF+DS+K RRILWGW
Sbjct: 289 KHDYYTIGTYDRVKDKFVPD--NGFKMDGT--APRYDYGKYYASKTFFDSAKNRRILWGW 348

Query: 363 VNESDSREDDIKKGWSGLQAVPRKIWLSKTGRQLTQWPVKEIEMLRRNHF-SLHHKELRG 422
            NES S EDD++KGWSG+Q +PRKIWL ++G+QL QWPV+E+E LR     +L +K L+ 
Sbjct: 349 TNESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKS 408

Query: 423 RSTMEILGGSASQVDVEVSFESPNLEEAESVETRWDDLDPQLLCSKRDASVNGRIGPFGL 482
            S +E+ G +A+Q DVEV F+  +LE+A+ +E  W   DPQL+CSK + SV   +GPFGL
Sbjct: 409 GSRLEVYGVTAAQADVEVLFKVRDLEKADVIEPSW--TDPQLICSKMNVSVKSGLGPFGL 468

Query: 483 LVLASNDLSEHTAIFFHILKAH---NRYVVLMCSDQSRSSFRKGVDKTTYGAFVDVDPCH 542
           +VLAS +L E+T+++F I KA    N+YVVLMCSDQSRSS ++  DKTTYGAFVD++P H
Sbjct: 469 MVLASKNLEEYTSVYFRIFKARQNSNKYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-H 528

Query: 543 KKISLRTLVDHTIVESFGGKGKTCITSRVYPTLAVANDARLYAFNNGTQTVVISSLEVWN 596
           + +SLR L+DH++VESFGGKG+ CITSRVYP LA+   + L+AFN G Q+V + +L  W+
Sbjct: 529 QPLSLRALIDHSVVESFGGKGRACITSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWS 583

BLAST of Bhi04G000459 vs. TAIR 10
Match: AT3G13790.2 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 603.6 bits (1555), Expect = 1.7e-172
Identity = 303/546 (55.49%), Postives = 389/546 (71.25%), Query Frame = 0

Query: 63  QIQRTSYHFQPLKNWMNDPNGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWV 122
           Q  RT +HFQP KNWM   NGPM YKGIYHLFYQ NP GAV+ + +VWAHS S DLINW 
Sbjct: 49  QPYRTGFHFQPPKNWM---NGPMIYKGIYHLFYQWNPKGAVWGN-IVWAHSTSTDLINWD 108

Query: 123 HLNHALEPSDPFDINGCWSGSVSFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEK 182
               A+ PS PFDINGCWSGS + L   KP+ILYTGID   QQVQN+A P N SDP+L +
Sbjct: 109 PHPPAIFPSAPFDINGCWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLRE 168

Query: 183 WRKSSQNPIIAPP--NGVERDKFRDPTTAWQGPDGEWRVAVGGHTSYGGAAMLYRSEDFV 242
           W+KS  NP++AP   NG+    FRDPTTAW G D +WRV +G      G A+ Y S+DF+
Sbjct: 169 WKKSPLNPLMAPDAVNGINASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFL 228

Query: 243 HWHQFRFPLYSSRDSGTWECPDFYPVMLNGTNGIDFSSGFGFG-----VKYVMKASFN-- 302
            W +   PL+    SG WECPDF+PV   G+NG++ SS   FG     +K+V+K S +  
Sbjct: 229 KWEKSPEPLHYDDGSGMWECPDFFPVTRFGSNGVETSS---FGEPNEILKHVLKISLDDT 288

Query: 303 SHDYYTLGSYATEKEKFTSDSSSGFDFRGTNLGLRYDYGKFYASKTFYDSSKKRRILWGW 362
            HDYYT+G+Y   K+KF  D  +GF   GT    RYDYGK+YASKTF+DS+K RRILWGW
Sbjct: 289 KHDYYTIGTYDRVKDKFVPD--NGFKMDGT--APRYDYGKYYASKTFFDSAKNRRILWGW 348

Query: 363 VNESDSREDDIKKGWSGLQAVPRKIWLSKTGRQLTQWPVKEIEMLRRNHF-SLHHKELRG 422
            NES S EDD++KGWSG+Q +PRKIWL ++G+QL QWPV+E+E LR     +L +K L+ 
Sbjct: 349 TNESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKS 408

Query: 423 RSTMEILGGSASQVDVEVSFESPNLEEAESVETRWDDLDPQLLCSKRDASVNGRIGPFGL 482
            S +E+ G +A+Q DVEV F+  +LE+A+ +E  W   DPQL+CSK + SV   +GPFGL
Sbjct: 409 GSRLEVYGVTAAQADVEVLFKVRDLEKADVIEPSW--TDPQLICSKMNVSVKSGLGPFGL 468

Query: 483 LVLASNDLSEHTAIFFHILKAH---NRYVVLMCSDQSRSSFRKGVDKTTYGAFVDVDPCH 542
           +VLAS +L E+T+++F I KA    N+YVVLMCSDQSRSS ++  DKTTYGAFVD++P H
Sbjct: 469 MVLASKNLEEYTSVYFRIFKARQNSNKYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-H 528

Query: 543 KKISLRTLVDHTIVESFGGKGKTCITSRVYPTLAVANDARLYAFNNGTQTVVISSLEVWN 596
           + +SLR L+DH++VESFGGKG+ CITSRVYP LA+   + L+AFN G Q+V + +L  W+
Sbjct: 529 QPLSLRALIDHSVVESFGGKGRACITSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWS 580

BLAST of Bhi04G000459 vs. TAIR 10
Match: AT1G55120.2 (beta-fructofuranosidase 5 )

HSP 1 Score: 589.0 bits (1517), Expect = 4.4e-168
Identity = 286/524 (54.58%), Postives = 372/524 (70.99%), Query Frame = 0

Query: 83  GPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEPSDPFDINGCWSG 142
           GPM YKGIYHLFYQ+NP GAV++ ++VW HS S DL+NW+    A  PS P DINGCWSG
Sbjct: 17  GPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSG 76

Query: 143 SVSFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEKWRKSSQNPIIA--PPNGVER 202
           SV+ L   KP+ILYTGID +  QVQN+A+P+N SDP+L +W K  QNP++     NG+  
Sbjct: 77  SVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINP 136

Query: 203 DKFRDPTTAWQGPDGEWRVAVGGHT-SYGGAAMLYRSEDFVHWHQFRFPLYSSRDSGTWE 262
           D+FRDPTTAW G DGEWRV VG  T    G A+LY+S DF +W Q   PL+    +G WE
Sbjct: 137 DRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWE 196

Query: 263 CPDFYPVMLNGTNGIDFSSGFGFGVKYVMKASF--NSHDYYTLGSYATEKEKFTSDSSSG 322
           CPDF+PV + G++G++ SS    G+K+V+K S     HDYYT+GSY  EK+ +  D    
Sbjct: 197 CPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPD---- 256

Query: 323 FDFRGTNLGLRYDYGKFYASKTFYDSSKKRRILWGWVNESDSREDDIKKGWSGLQAVPRK 382
             F       R DYGK+YASKTFYD  KKRRILWGWVNES   +DDI+KGWSGLQ+ PRK
Sbjct: 257 LGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRK 316

Query: 383 IWLSKTGRQLTQWPVKEIEMLRRNHFSLHHKELRGRSTMEILGGSASQVDVEVSFESPNL 442
           IWL ++G++L QWP++EIE LR    +   K L+  ST+++ G +A+Q DVEVSF+   L
Sbjct: 317 IWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKEL 376

Query: 443 EEAESVETRWDDLDPQLLCSKRDASVNGRIGPFGLLVLASNDLSEHTAIFFHILKAHN-- 502
           E+A+ +E  W   DPQ +CS+ D SV   +GPFGL+VLASND+ E+T+++F I K+++  
Sbjct: 377 EKADVIEPSW--TDPQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDT 436

Query: 503 ----RYVVLMCSDQSRSSFRKGVDKTTYGAFVDVDPCHKKISLRTLVDHTIVESFGGKGK 562
               +YVVLMCSDQSRSS     DK+T+GAFV +DP H+ ISLRTL+DH+IVES+GG G+
Sbjct: 437 NKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGR 496

Query: 563 TCITSRVYPTLAVANDARLYAFNNGTQTVVISSLEVWNMSNAQI 596
           TCITSRVYP LA+  +A L+ FN GTQ+V I +L  W++ +AQI
Sbjct: 497 TCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQI 534

BLAST of Bhi04G000459 vs. TAIR 10
Match: AT1G55120.3 (beta-fructofuranosidase 5 )

HSP 1 Score: 589.0 bits (1517), Expect = 4.4e-168
Identity = 286/525 (54.48%), Postives = 373/525 (71.05%), Query Frame = 0

Query: 82  NGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEPSDPFDINGCWS 141
           +GPM YKGIYHLFYQ+NP GAV++ ++VW HS S DL+NW+    A  PS P DINGCWS
Sbjct: 40  SGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWS 99

Query: 142 GSVSFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEKWRKSSQNPIIA--PPNGVE 201
           GSV+ L   KP+ILYTGID +  QVQN+A+P+N SDP+L +W K  QNP++     NG+ 
Sbjct: 100 GSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGIN 159

Query: 202 RDKFRDPTTAWQGPDGEWRVAVGGHT-SYGGAAMLYRSEDFVHWHQFRFPLYSSRDSGTW 261
            D+FRDPTTAW G DGEWRV VG  T    G A+LY+S DF +W Q   PL+    +G W
Sbjct: 160 PDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMW 219

Query: 262 ECPDFYPVMLNGTNGIDFSSGFGFGVKYVMKASF--NSHDYYTLGSYATEKEKFTSDSSS 321
           ECPDF+PV + G++G++ SS    G+K+V+K S     HDYYT+GSY  EK+ +  D   
Sbjct: 220 ECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPD--- 279

Query: 322 GFDFRGTNLGLRYDYGKFYASKTFYDSSKKRRILWGWVNESDSREDDIKKGWSGLQAVPR 381
              F       R DYGK+YASKTFYD  KKRRILWGWVNES   +DDI+KGWSGLQ+ PR
Sbjct: 280 -LGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPR 339

Query: 382 KIWLSKTGRQLTQWPVKEIEMLRRNHFSLHHKELRGRSTMEILGGSASQVDVEVSFESPN 441
           KIWL ++G++L QWP++EIE LR    +   K L+  ST+++ G +A+Q DVEVSF+   
Sbjct: 340 KIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKE 399

Query: 442 LEEAESVETRWDDLDPQLLCSKRDASVNGRIGPFGLLVLASNDLSEHTAIFFHILKAHN- 501
           LE+A+ +E  W   DPQ +CS+ D SV   +GPFGL+VLASND+ E+T+++F I K+++ 
Sbjct: 400 LEKADVIEPSW--TDPQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDD 459

Query: 502 -----RYVVLMCSDQSRSSFRKGVDKTTYGAFVDVDPCHKKISLRTLVDHTIVESFGGKG 561
                +YVVLMCSDQSRSS     DK+T+GAFV +DP H+ ISLRTL+DH+IVES+GG G
Sbjct: 460 TNKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGG 519

Query: 562 KTCITSRVYPTLAVANDARLYAFNNGTQTVVISSLEVWNMSNAQI 596
           +TCITSRVYP LA+  +A L+ FN GTQ+V I +L  W++ +AQI
Sbjct: 520 RTCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQI 558

BLAST of Bhi04G000459 vs. ExPASy Swiss-Prot
Match: Q67XZ3 (Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3702 GN=CWINV3 PE=1 SV=2)

HSP 1 Score: 627.5 bits (1617), Expect = 1.6e-178
Identity = 303/544 (55.70%), Postives = 389/544 (71.51%), Query Frame = 0

Query: 63  QIQRTSYHFQPLKNWMNDPNGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWV 122
           Q  RT YHFQPLKNWMNDPNGPM YKGIYHLFYQ+NP GAV++ ++VW HS S DL+NW+
Sbjct: 36  QPYRTGYHFQPLKNWMNDPNGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWI 95

Query: 123 HLNHALEPSDPFDINGCWSGSVSFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEK 182
               A  PS P DINGCWSGSV+ L   KP+ILYTGID +  QVQN+A+P+N SDP+L +
Sbjct: 96  SQPPAFNPSQPSDINGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLRE 155

Query: 183 WRKSSQNPIIA--PPNGVERDKFRDPTTAWQGPDGEWRVAVGGHT-SYGGAAMLYRSEDF 242
           W K  QNP++     NG+  D+FRDPTTAW G DGEWRV VG  T    G A+LY+S DF
Sbjct: 156 WSKPPQNPLMTTNAVNGINPDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDF 215

Query: 243 VHWHQFRFPLYSSRDSGTWECPDFYPVMLNGTNGIDFSSGFGFGVKYVMKASF--NSHDY 302
            +W Q   PL+    +G WECPDF+PV + G++G++ SS    G+K+V+K S     HDY
Sbjct: 216 FNWTQSMKPLHYEDLTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDY 275

Query: 303 YTLGSYATEKEKFTSDSSSGFDFRGTNLGLRYDYGKFYASKTFYDSSKKRRILWGWVNES 362
           YT+GSY  EK+ +  D      F       R DYGK+YASKTFYD  KKRRILWGWVNES
Sbjct: 276 YTIGSYDREKDVYVPD----LGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNES 335

Query: 363 DSREDDIKKGWSGLQAVPRKIWLSKTGRQLTQWPVKEIEMLRRNHFSLHHKELRGRSTME 422
              +DDI+KGWSGLQ+ PRKIWL ++G++L QWP++EIE LR    +   K L+  ST++
Sbjct: 336 SPAKDDIEKGWSGLQSFPRKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQ 395

Query: 423 ILGGSASQVDVEVSFESPNLEEAESVETRWDDLDPQLLCSKRDASVNGRIGPFGLLVLAS 482
           + G +A+Q DVEVSF+   LE+A+ +E  W   DPQ +CS+ D SV   +GPFGL+VLAS
Sbjct: 396 VHGVTAAQADVEVSFKVKELEKADVIEPSW--TDPQKICSQGDLSVMSGLGPFGLMVLAS 455

Query: 483 NDLSEHTAIFFHILKAHN------RYVVLMCSDQSRSSFRKGVDKTTYGAFVDVDPCHKK 542
           ND+ E+T+++F I K+++      +YVVLMCSDQSRSS     DK+T+GAFV +DP H+ 
Sbjct: 456 NDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQT 515

Query: 543 ISLRTLVDHTIVESFGGKGKTCITSRVYPTLAVANDARLYAFNNGTQTVVISSLEVWNMS 596
           ISLRTL+DH+IVES+GG G+TCITSRVYP LA+  +A L+ FN GTQ+V I +L  W++ 
Sbjct: 516 ISLRTLIDHSIVESYGGGGRTCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLK 573

BLAST of Bhi04G000459 vs. ExPASy Swiss-Prot
Match: Q43866 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3702 GN=CWINV1 PE=1 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 4.7e-175
Identity = 306/546 (56.04%), Postives = 392/546 (71.79%), Query Frame = 0

Query: 63  QIQRTSYHFQPLKNWMNDPNGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWV 122
           Q  RT +HFQP KNWMNDPNGPM YKGIYHLFYQ NP GAV+ + +VWAHS S DLINW 
Sbjct: 49  QPYRTGFHFQPPKNWMNDPNGPMIYKGIYHLFYQWNPKGAVWGN-IVWAHSTSTDLINWD 108

Query: 123 HLNHALEPSDPFDINGCWSGSVSFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEK 182
               A+ PS PFDINGCWSGS + L   KP+ILYTGID   QQVQN+A P N SDP+L +
Sbjct: 109 PHPPAIFPSAPFDINGCWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLRE 168

Query: 183 WRKSSQNPIIAPP--NGVERDKFRDPTTAWQGPDGEWRVAVGGHTSYGGAAMLYRSEDFV 242
           W+KS  NP++AP   NG+    FRDPTTAW G D +WRV +G      G A+ Y S+DF+
Sbjct: 169 WKKSPLNPLMAPDAVNGINASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFL 228

Query: 243 HWHQFRFPLYSSRDSGTWECPDFYPVMLNGTNGIDFSSGFGFG-----VKYVMKASFN-- 302
            W +   PL+    SG WECPDF+PV   G+NG++ SS   FG     +K+V+K S +  
Sbjct: 229 KWEKSPEPLHYDDGSGMWECPDFFPVTRFGSNGVETSS---FGEPNEILKHVLKISLDDT 288

Query: 303 SHDYYTLGSYATEKEKFTSDSSSGFDFRGTNLGLRYDYGKFYASKTFYDSSKKRRILWGW 362
            HDYYT+G+Y   K+KF  D  +GF   GT    RYDYGK+YASKTF+DS+K RRILWGW
Sbjct: 289 KHDYYTIGTYDRVKDKFVPD--NGFKMDGT--APRYDYGKYYASKTFFDSAKNRRILWGW 348

Query: 363 VNESDSREDDIKKGWSGLQAVPRKIWLSKTGRQLTQWPVKEIEMLRRNHF-SLHHKELRG 422
            NES S EDD++KGWSG+Q +PRKIWL ++G+QL QWPV+E+E LR     +L +K L+ 
Sbjct: 349 TNESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKS 408

Query: 423 RSTMEILGGSASQVDVEVSFESPNLEEAESVETRWDDLDPQLLCSKRDASVNGRIGPFGL 482
            S +E+ G +A+Q DVEV F+  +LE+A+ +E  W   DPQL+CSK + SV   +GPFGL
Sbjct: 409 GSRLEVYGVTAAQADVEVLFKVRDLEKADVIEPSW--TDPQLICSKMNVSVKSGLGPFGL 468

Query: 483 LVLASNDLSEHTAIFFHILKAH---NRYVVLMCSDQSRSSFRKGVDKTTYGAFVDVDPCH 542
           +VLAS +L E+T+++F I KA    N+YVVLMCSDQSRSS ++  DKTTYGAFVD++P H
Sbjct: 469 MVLASKNLEEYTSVYFRIFKARQNSNKYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-H 528

Query: 543 KKISLRTLVDHTIVESFGGKGKTCITSRVYPTLAVANDARLYAFNNGTQTVVISSLEVWN 596
           + +SLR L+DH++VESFGGKG+ CITSRVYP LA+   + L+AFN G Q+V + +L  W+
Sbjct: 529 QPLSLRALIDHSVVESFGGKGRACITSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWS 583

BLAST of Bhi04G000459 vs. ExPASy Swiss-Prot
Match: Q70XE6 (Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 3.5e-170
Identity = 290/536 (54.10%), Postives = 380/536 (70.90%), Query Frame = 0

Query: 66  RTSYHFQPLKNWMNDPNGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLN 125
           RT+YHFQ  KNWMNDPNGPM YKGIYHLFYQ+ P   V+++++VW HS S DLINW    
Sbjct: 56  RTAYHFQSPKNWMNDPNGPMIYKGIYHLFYQYYPYDPVWHTEIVWGHSTSTDLINWTQQP 115

Query: 126 HALEPSDPFDINGCWSGSVSFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEKWRK 185
            AL PS+P+DINGCWSGS++ L +NKP+ILYTGI++   QVQNLA+P N SDP+L++W K
Sbjct: 116 IALSPSEPYDINGCWSGSITILPQNKPVILYTGINNKNYQVQNLALPKNLSDPYLKEWIK 175

Query: 186 SSQNPIIAPP----NGVERDKFRDPTTAWQGPDGEWRVAVGGHTSYGGAAMLYRSEDFVH 245
             QNP++A      N +    FRDP+TAWQ  DG+WRV VG      G A+L+ S+DFV 
Sbjct: 176 LPQNPLMAGTPTNNNNINASSFRDPSTAWQLSDGKWRVIVGTQQGKRGLAVLFTSDDFVK 235

Query: 246 WHQFRFPLYSSRDSGTWECPDFYPVMLNGTNGIDFSSGFGFGVKYVMKASF--NSHDYYT 305
           W+    PL+S+  +G WECPDF+PV +  + G D +S  G  VK+V+K S     ++YYT
Sbjct: 236 WNNTGNPLHSTEGNGIWECPDFFPVYVGKSLGAD-TSIIGDDVKHVLKLSLFDTQYEYYT 295

Query: 306 LGSYATEKEKFTSDSSSGFDFRGTNLGLRYDYGKFYASKTFYDSSKKRRILWGWVNESDS 365
           +G Y  EK+ +  D  S      ++LGLRYDYGKFYASK+F+D    RRILWGWVNES  
Sbjct: 296 IGRYDIEKDIYVPDEGS----IESDLGLRYDYGKFYASKSFFDDETNRRILWGWVNESSI 355

Query: 366 REDDIKKGWSGLQAVPRKIWLSKTGRQLTQWPVKEIEMLRRNHFSLHHKELRGRSTMEIL 425
           + DDIKKGWSG+QA+PR + L K+G+QL QWP+ E++MLR N   L  + ++G S +EI 
Sbjct: 356 QADDIKKGWSGVQAIPRTVVLDKSGKQLVQWPLAEVDMLRENDVELPSQVIKGGSLVEIS 415

Query: 426 GGSASQVDVEVSFESPNLEEAESVETRWDDLDPQLLCSKRDASVNGRIGPFGLLVLASND 485
             +ASQ DVE+SF+ P     E +++     +PQ+LCS++ AS+ GR GPFGLL LAS  
Sbjct: 416 QITASQADVEISFKIPESNYVEELDSTC--TNPQILCSQKGASIKGRFGPFGLLTLASMG 475

Query: 486 LSEHTAIFFHILKAHNRYVVLMCSDQSRSSFRKGVDKTTYGAFVDVDPCHKKISLRTLVD 545
           L E+TA+FF I K  N+YVVLMCSDQ+RSS     DK ++G FVDVDP ++ +SLR L+D
Sbjct: 476 LEEYTAVFFRIFKGPNKYVVLMCSDQTRSSLNPTTDKLSFGIFVDVDPINEDLSLRILID 535

Query: 546 HTIVESFGGKGKTCITSRVYPTLAVANDARLYAFNNGTQTVVISSLEVWNMSNAQI 596
           H+IVESF  KGK+CIT+RVYPT+A+ + A+LY FNNGT+ V I+ L  W+M  AQI
Sbjct: 536 HSIVESFSAKGKSCITARVYPTMAINDKAKLYVFNNGTEDVKITKLSAWSMKKAQI 584

BLAST of Bhi04G000459 vs. ExPASy Swiss-Prot
Match: Q43089 (Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 PE=2 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 5.8e-165
Identity = 285/526 (54.18%), Postives = 368/526 (69.96%), Query Frame = 0

Query: 51  SHEYANGVHIPQQIQRTSYHFQPLKNWMNDPNGPMFYKGIYHLFYQHNPNGAVFNSKMVW 110
           SH  +   H P    RT+YHFQPLKNW+NDPNGPM Y G YHLFYQ+NP GAV+ + +VW
Sbjct: 35  SHHSSKSNHQP---YRTAYHFQPLKNWINDPNGPMRYGGFYHLFYQYNPKGAVWGN-IVW 94

Query: 111 AHSISHDLINWVHLNHALEPSDPFDINGCWSGSVSFLLKNKPLILYTGIDSSFQQVQNLA 170
           AHS+S DL+NW  L+HA+ PS P DI GCWSGS + L   KP ILYTGID +  QVQN+A
Sbjct: 95  AHSVSKDLVNWTPLDHAIHPSQPSDIKGCWSGSATILPGGKPAILYTGIDPNNHQVQNIA 154

Query: 171 IPINFSDPFLEKWRKSSQNPIIAP--PNGVERDKFRDPTTAWQGPDGEWRVAVGGHTSYG 230
           IP N SDP L +W+KS +NP++ P   N +    FRDPTT+W G DG WRV +G      
Sbjct: 155 IPKNMSDPLLREWKKSPKNPLMEPTIANKINSSSFRDPTTSWLGKDGFWRVLIGSKIDTK 214

Query: 231 GAAMLYRSEDFVHWHQFRFPLYSSRDSGTWECPDFYPVMLNG--TNGIDFSSGFGFGVKY 290
           G A+LY+S++FV W + + PL+S+  +G WECPDFYPV+       G+D S      V++
Sbjct: 215 GMAILYKSKNFVDWVEAKHPLHSAEGTGMWECPDFYPVLDKNLLRTGVDTSRNGDDDVRH 274

Query: 291 VMKASFN--SHDYYTLGSYATEKEKFTSDSSSGFDFRGTNLGLRYDYGKFYASKTFYDSS 350
           V+K S +   HD+Y +GSY   K+ F  +  +GF+  G    LRYDYGK+YASKTF+D  
Sbjct: 275 VLKVSLDDTKHDHYLIGSYDVVKDVFVPE--NGFEDNG--FVLRYDYGKYYASKTFFDDG 334

Query: 351 KKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIWLSKTGRQLTQWPVKEIEMLRRNHFS 410
           K RRIL GWVNES S  DD+KKGWSG+  +PR+IWL ++G+QL QWPVKEIE LR N  +
Sbjct: 335 KNRRILLGWVNESSSVADDVKKGWSGIHTIPREIWLHESGKQLVQWPVKEIENLRMNPVN 394

Query: 411 LHHKELRGRSTMEILGGSASQVDVEVSFESPNLEEAESVETRWDDLDPQLLCSKRDASVN 470
              K ++G   + I G  + Q DVE+SFE  +L + ES+  +W  +DPQLLCS++ A V 
Sbjct: 395 WPTKVIKGGERISITGVDSVQADVEISFEISDLGKVESLR-KW--IDPQLLCSQKGAGVK 454

Query: 471 GRIGPFGLLVLASNDLSEHTAIFFHILKAHNRYVVLMCSDQSRSSFRKGVDKTTYGAFVD 530
           G +GPFGLLV AS  L E+TA+FF I K  ++ +VLMCSDQSRSS  K  D T+YG FVD
Sbjct: 455 GGVGPFGLLVFASQGLKEYTAVFFRIFKYQDKNLVLMCSDQSRSSLNKDNDMTSYGTFVD 514

Query: 531 VDPCHKKISLRTLVDHTIVESFGGKGKTCITSRVYPTLAVANDARL 571
           VDP H+K+SLRTL+DH++VESFGG+G+ C+T+RVYPTLA+ + A L
Sbjct: 515 VDPLHEKLSLRTLIDHSVVESFGGEGRACVTARVYPTLAIHDKAML 549

BLAST of Bhi04G000459 vs. ExPASy Swiss-Prot
Match: Q9LIB9 (Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3702 GN=CWINV5 PE=2 SV=2)

HSP 1 Score: 579.7 bits (1493), Expect = 3.8e-164
Identity = 293/545 (53.76%), Postives = 372/545 (68.26%), Query Frame = 0

Query: 59  HIPQQIQRTSYHFQPLKNWMNDPNGPMFYKGIYHLFYQHNPNGAVFN-SKMVWAHSISHD 118
           +I  Q  RT YHFQP KNWMNDPNGPM YKGIYHLFYQ N NGAV + +K VW H+ S D
Sbjct: 36  NIKNQPYRTGYHFQPPKNWMNDPNGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHATSTD 95

Query: 119 LINWVHLNHALEPSDPFDINGCWSGSVSFLLKNKPLILYTGIDSSFQQVQNLAIPINFSD 178
           LINW+ L+ A+ PS P DINGCWSGSV+ L   KP+ILYTG D   +QVQNL  P N +D
Sbjct: 96  LINWITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKNLTD 155

Query: 179 PFLEKWRKSSQNPII--APPNGVERDKFRDPTTAWQGPDGEWRVAVGGHTSYGGAAMLYR 238
           P+L  W KS +NP++  +P N +    FRDPTTAW G DG WR+  G      G A+L+ 
Sbjct: 156 PYLRHWTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAILHT 215

Query: 239 SEDFVHWHQFRFPLYSSRDSGTWECPDFYPVMLNGTNGIDFSSGFGFGVKYVMKASFNS- 298
           S+DFV W Q   PL+    +G WECPDF+PV    + G+D S   G  VK+V+K S    
Sbjct: 216 SKDFVIWKQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKVSLTDT 275

Query: 299 -HDYYTLGSYATEKEKFTSDSSSGFDFRGTNLGLRYDYGKFYASKTFYDSSKKRRILWGW 358
            +DYYT+G+Y   ++ +  D      F       RYDYGKFYASKTFYDS  +RRILWGW
Sbjct: 276 FNDYYTIGTYDEVRDVYVPDKG----FVQDETAPRYDYGKFYASKTFYDSVNQRRILWGW 335

Query: 359 VNESDSREDDIKKGWSGLQAVPRKIWLSKTGRQLTQWPVKEIEMLRRNHFSLHHKELRGR 418
           VNES   +D+IKKGW+GLQA+PRK+WL ++G++L QWPVKEIE LR       +K L+G 
Sbjct: 336 VNESSPEKDNIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKGG 395

Query: 419 STMEILGGSASQVDVEVSFESP--NLEEAESVETRWDDLDPQLLCSKRDASVNGRIGPFG 478
           S ME+ G +A Q DVEV F+    +LE+A+ +E  W   DPQL+CS+R+AS    +GPFG
Sbjct: 396 SVMEVHGVTAPQADVEVFFKVSGFDLEKADVIEPGW--TDPQLICSQRNAS--SGLGPFG 455

Query: 479 LLVLASNDLSEHTAIFFHILKA---HNRYVVLMCSDQSRSSFRKGVDKTTYGAFVDVDPC 538
           L+VLAS ++ E+T++   I +A      +VV+MCSDQS SS  KG DKTTYGAF+D+ P 
Sbjct: 456 LMVLASKNMEEYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISP- 515

Query: 539 HKKISLRTLVDHTIVESFGGKGKTCITSRVYPTLAVANDARLYAFNNGTQTVVISSLEVW 594
           ++ ISLRTL+D +IVESFGGKGKTCITSRVYP LA+     L+AFN G+Q V + SL  W
Sbjct: 516 YQPISLRTLIDKSIVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSAW 571

BLAST of Bhi04G000459 vs. NCBI nr
Match: XP_008444460.1 (PREDICTED: LOW QUALITY PROTEIN: beta-fructofuranosidase, insoluble isoenzyme CWINV3-like [Cucumis melo])

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 511/579 (88.26%), Postives = 545/579 (94.13%), Query Frame = 0

Query: 19  DKMPTKCLQMGKCGLIALYFLLVISNRIGFVVSHEYANGVH-IPQQIQRTSYHFQPLKNW 78
           DKMP K L+MGK GL+A + LL+ SNR+GFVVSHEYAN VH  PQQ+QRTSYHFQPLKNW
Sbjct: 2   DKMPPKRLEMGKYGLVASFILLLASNRMGFVVSHEYANEVHSTPQQVQRTSYHFQPLKNW 61

Query: 79  MNDPNGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEPSDPFDIN 138
           MNDPNGPMFYKGIYH FYQHNPNGAVFNSKMVWAHSIS+DLINWVHLNHALEP+DPFDIN
Sbjct: 62  MNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDIN 121

Query: 139 GCWSGSVSFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEKWRKSSQNPIIAPPNG 198
           GCWSGSVSFL KNKP ILYTGIDSSFQQVQNLAIP N+SDPFLEKWRKS QNPIIAPP+G
Sbjct: 122 GCWSGSVSFLPKNKPRILYTGIDSSFQQVQNLAIPTNYSDPFLEKWRKSPQNPIIAPPDG 181

Query: 199 VERDKFRDPTTAWQGPDGEWRVAVGGHTSYGGAAMLYRSEDFVHWHQFRFPLYSSRDSGT 258
            ER++FRDPTTAWQGPDGEWRVA+GG TSYGGAAMLYRSEDFV WHQFRFPLYSS+DSGT
Sbjct: 182 AERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGT 241

Query: 259 WECPDFYPVMLNGTNGIDFSSGFGFGVKYVMKASFNSHDYYTLGSYATEKEKFTSDSSSG 318
           WECPDFYPVMLNGTNGI FSSGFG GVK+VMKASFNS D+YTLGSY  EKEKFTSD   G
Sbjct: 242 WECPDFYPVMLNGTNGIGFSSGFGVGVKHVMKASFNSRDHYTLGSYVPEKEKFTSDHGLG 301

Query: 319 FDFRGTNLGLRYDYGKFYASKTFYDSSKKRRILWGWVNESDSREDDIKKGWSGLQAVPRK 378
           FDFRG NLGLRYDYGKFYASKTFYDSS+KRRILWGWVNESDSR+DDI KGWSGLQA+PRK
Sbjct: 302 FDFRGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRK 361

Query: 379 IWLSKTGRQLTQWPVKEIEMLRRNHFSLHHKELRGRSTMEILGGSASQVDVEVSFESPNL 438
           IWLSKTGRQL QWPVKEI+MLRRNHFSLHHKELRGRSTME+LGGSASQVDVEVSF  P+L
Sbjct: 362 IWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFALPHL 421

Query: 439 EEAESVETRWDDLDPQLLCSKRDASVNGRIGPFGLLVLASNDLSEHTAIFFHILKAHNRY 498
           EEAESV+TRW  LDPQ+LCSKRDAS+NGR+GPFGLLVLASNDLSEHTAI+FH+LKAHNRY
Sbjct: 422 EEAESVDTRWGVLDPQVLCSKRDASMNGRVGPFGLLVLASNDLSEHTAIYFHVLKAHNRY 481

Query: 499 VVLMCSDQSRSSFRKGVDKTTYGAFVDVDPCHKKISLRTLVDHTIVESFGGKGKTCITSR 558
           VVLMCSD+SRSSFRKGVDKTTYGAFVD+DP +KKISLRTLVDH+IVESFGGKGKTCITSR
Sbjct: 482 VVLMCSDESRSSFRKGVDKTTYGAFVDIDPRYKKISLRTLVDHSIVESFGGKGKTCITSR 541

Query: 559 VYPTLAVANDARLYAFNNGTQTVVISSLEVWNMSNAQIK 597
           VYPTLAV NDARLYAFNNGTQTVVISSL+VWNMSNA+IK
Sbjct: 542 VYPTLAVNNDARLYAFNNGTQTVVISSLKVWNMSNAEIK 580

BLAST of Bhi04G000459 vs. NCBI nr
Match: XP_038884233.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV3-like [Benincasa hispida])

HSP 1 Score: 1068.9 bits (2763), Expect = 1.6e-308
Identity = 512/512 (100.00%), Postives = 512/512 (100.00%), Query Frame = 0

Query: 85  MFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEPSDPFDINGCWSGSV 144
           MFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEPSDPFDINGCWSGSV
Sbjct: 1   MFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEPSDPFDINGCWSGSV 60

Query: 145 SFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEKWRKSSQNPIIAPPNGVERDKFR 204
           SFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEKWRKSSQNPIIAPPNGVERDKFR
Sbjct: 61  SFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEKWRKSSQNPIIAPPNGVERDKFR 120

Query: 205 DPTTAWQGPDGEWRVAVGGHTSYGGAAMLYRSEDFVHWHQFRFPLYSSRDSGTWECPDFY 264
           DPTTAWQGPDGEWRVAVGGHTSYGGAAMLYRSEDFVHWHQFRFPLYSSRDSGTWECPDFY
Sbjct: 121 DPTTAWQGPDGEWRVAVGGHTSYGGAAMLYRSEDFVHWHQFRFPLYSSRDSGTWECPDFY 180

Query: 265 PVMLNGTNGIDFSSGFGFGVKYVMKASFNSHDYYTLGSYATEKEKFTSDSSSGFDFRGTN 324
           PVMLNGTNGIDFSSGFGFGVKYVMKASFNSHDYYTLGSYATEKEKFTSDSSSGFDFRGTN
Sbjct: 181 PVMLNGTNGIDFSSGFGFGVKYVMKASFNSHDYYTLGSYATEKEKFTSDSSSGFDFRGTN 240

Query: 325 LGLRYDYGKFYASKTFYDSSKKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIWLSKTG 384
           LGLRYDYGKFYASKTFYDSSKKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIWLSKTG
Sbjct: 241 LGLRYDYGKFYASKTFYDSSKKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIWLSKTG 300

Query: 385 RQLTQWPVKEIEMLRRNHFSLHHKELRGRSTMEILGGSASQVDVEVSFESPNLEEAESVE 444
           RQLTQWPVKEIEMLRRNHFSLHHKELRGRSTMEILGGSASQVDVEVSFESPNLEEAESVE
Sbjct: 301 RQLTQWPVKEIEMLRRNHFSLHHKELRGRSTMEILGGSASQVDVEVSFESPNLEEAESVE 360

Query: 445 TRWDDLDPQLLCSKRDASVNGRIGPFGLLVLASNDLSEHTAIFFHILKAHNRYVVLMCSD 504
           TRWDDLDPQLLCSKRDASVNGRIGPFGLLVLASNDLSEHTAIFFHILKAHNRYVVLMCSD
Sbjct: 361 TRWDDLDPQLLCSKRDASVNGRIGPFGLLVLASNDLSEHTAIFFHILKAHNRYVVLMCSD 420

Query: 505 QSRSSFRKGVDKTTYGAFVDVDPCHKKISLRTLVDHTIVESFGGKGKTCITSRVYPTLAV 564
           QSRSSFRKGVDKTTYGAFVDVDPCHKKISLRTLVDHTIVESFGGKGKTCITSRVYPTLAV
Sbjct: 421 QSRSSFRKGVDKTTYGAFVDVDPCHKKISLRTLVDHTIVESFGGKGKTCITSRVYPTLAV 480

Query: 565 ANDARLYAFNNGTQTVVISSLEVWNMSNAQIK 597
           ANDARLYAFNNGTQTVVISSLEVWNMSNAQIK
Sbjct: 481 ANDARLYAFNNGTQTVVISSLEVWNMSNAQIK 512

BLAST of Bhi04G000459 vs. NCBI nr
Match: XP_004142928.3 (beta-fructofuranosidase, insoluble isoenzyme CWINV3 isoform X1 [Cucumis sativus] >KAE8652051.1 hypothetical protein Csa_018697 [Cucumis sativus])

HSP 1 Score: 1067.0 bits (2758), Expect = 5.9e-308
Identity = 505/578 (87.37%), Postives = 540/578 (93.43%), Query Frame = 0

Query: 19  DKMPTKCLQMGKCGLIALYFLLVISNRIGFVVSHEYANGVHIPQQIQRTSYHFQPLKNWM 78
           DKMP K L++GK GL+  + +L+  NRIGFVVSHEYAN VH P Q+QRTSYHFQPLKNWM
Sbjct: 31  DKMPPKRLEIGKYGLVVSFVILLTCNRIGFVVSHEYANEVHTP-QVQRTSYHFQPLKNWM 90

Query: 79  NDPNGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEPSDPFDING 138
           NDPNGPMFYKGIYH FYQHNPNGAVFNSKMVWAHSIS+DLINWVHLNHALEP+DPFDING
Sbjct: 91  NDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDING 150

Query: 139 CWSGSVSFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEKWRKSSQNPIIAPPNGV 198
           CWSGSVSFL +NKP ILYTGIDSS QQVQNLAIP N+SDPFLEKWRK SQNPIIAPP+G+
Sbjct: 151 CWSGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIAPPDGL 210

Query: 199 ERDKFRDPTTAWQGPDGEWRVAVGGHTSYGGAAMLYRSEDFVHWHQFRFPLYSSRDSGTW 258
           ER++FRDPTTAWQGPDGEWRVA+GG TSYGGAAMLYRSEDFV WHQFRFPLYSS+DSGTW
Sbjct: 211 ERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTW 270

Query: 259 ECPDFYPVMLNGTNGIDFSSGFGFGVKYVMKASFNSHDYYTLGSYATEKEKFTSDSSSGF 318
           ECPDFYPVMLNGTNGI FSSGFG GVKYVMKASFNS D+YTLGSY  EKEKFTSD   GF
Sbjct: 271 ECPDFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTLGSYVPEKEKFTSDYGPGF 330

Query: 319 DFRGTNLGLRYDYGKFYASKTFYDSSKKRRILWGWVNESDSREDDIKKGWSGLQAVPRKI 378
           DF+G NLGLRYDYGKFYASKTFYDSS+KRRILWGWVNESDSR+DDI KGWSGLQA+PRKI
Sbjct: 331 DFKGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRKI 390

Query: 379 WLSKTGRQLTQWPVKEIEMLRRNHFSLHHKELRGRSTMEILGGSASQVDVEVSFESPNLE 438
           WLSKTGRQL QWPVKEI+MLRRNHFSLHHKELRGRSTME+LGGSASQVDVEVSFE P LE
Sbjct: 391 WLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPYLE 450

Query: 439 EAESVETRWDDLDPQLLCSKRDASVNGRIGPFGLLVLASNDLSEHTAIFFHILKAHNRYV 498
           EAESV+TRW  LDPQ+LCSKRDASVNGR+GPFGLLVLASNDLSEHTAI+FHILKAHNRYV
Sbjct: 451 EAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHNRYV 510

Query: 499 VLMCSDQSRSSFRKGVDKTTYGAFVDVDPCHKKISLRTLVDHTIVESFGGKGKTCITSRV 558
           VLMCSDQSRSSFRK VDKT +GAFVD+DPC+KKISLRTLVDH+IVESFGGKGKTCITSRV
Sbjct: 511 VLMCSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSRV 570

Query: 559 YPTLAVANDARLYAFNNGTQTVVISSLEVWNMSNAQIK 597
           YPTLAV N+ARLYAFNNGTQTVVISSL+ WNMSNA+IK
Sbjct: 571 YPTLAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEIK 607

BLAST of Bhi04G000459 vs. NCBI nr
Match: ALS30215.1 (cell wall invertase 4 [Cucumis sativus])

HSP 1 Score: 1062.4 bits (2746), Expect = 1.5e-306
Identity = 503/576 (87.33%), Postives = 538/576 (93.40%), Query Frame = 0

Query: 21  MPTKCLQMGKCGLIALYFLLVISNRIGFVVSHEYANGVHIPQQIQRTSYHFQPLKNWMND 80
           MP K L++GK GL+  + +L+  NRIGFVVSHEYAN VH P Q+QRTSYHFQPLKNWMND
Sbjct: 1   MPPKRLEIGKYGLVVSFVILLTCNRIGFVVSHEYANEVHTP-QVQRTSYHFQPLKNWMND 60

Query: 81  PNGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEPSDPFDINGCW 140
           PNGPMFYKGIYH FYQHNPNGAVFNSKMVWAHSIS+DLINWVHLNHALEP+DPFDINGCW
Sbjct: 61  PNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDINGCW 120

Query: 141 SGSVSFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEKWRKSSQNPIIAPPNGVER 200
           SGSVSFL +NKP ILYTGIDSS QQVQNLAIP N+SDPFLEKWRK SQNPIIAPP+G+ER
Sbjct: 121 SGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIAPPDGLER 180

Query: 201 DKFRDPTTAWQGPDGEWRVAVGGHTSYGGAAMLYRSEDFVHWHQFRFPLYSSRDSGTWEC 260
           ++FRDPTTAWQGPDGEWRVA+GG TSYGGAAMLYRSEDFV WHQFRFPLYSS+DSGTWEC
Sbjct: 181 NRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWEC 240

Query: 261 PDFYPVMLNGTNGIDFSSGFGFGVKYVMKASFNSHDYYTLGSYATEKEKFTSDSSSGFDF 320
           PDFYPVMLNGTNGI FSSGFG GVKYVMKASFNS D+YTLGSY  EKEKFTSD   GFDF
Sbjct: 241 PDFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTLGSYVPEKEKFTSDYGPGFDF 300

Query: 321 RGTNLGLRYDYGKFYASKTFYDSSKKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIWL 380
           +G NLGLRYDYGKFYASKTFYDSS+KRRILWGWVNESDSR+DDI KGWSGLQA+PRKIWL
Sbjct: 301 KGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRKIWL 360

Query: 381 SKTGRQLTQWPVKEIEMLRRNHFSLHHKELRGRSTMEILGGSASQVDVEVSFESPNLEEA 440
           SKTGRQL QWPVKEI+MLRRNHFSLHHKELRGRSTME+LGGSASQVDVEVSFE P LEEA
Sbjct: 361 SKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPYLEEA 420

Query: 441 ESVETRWDDLDPQLLCSKRDASVNGRIGPFGLLVLASNDLSEHTAIFFHILKAHNRYVVL 500
           ESV+TRW  LDPQ+LCSKRDASVNGR+GPFGLLVLASNDLSEHTAI+FHILKAHNRYVVL
Sbjct: 421 ESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHNRYVVL 480

Query: 501 MCSDQSRSSFRKGVDKTTYGAFVDVDPCHKKISLRTLVDHTIVESFGGKGKTCITSRVYP 560
           MCSDQSRSSFRK VDKT +GAFVD+DPC+KKISLRTLVDH+IVESFGGKGKTCITSRVYP
Sbjct: 481 MCSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSRVYP 540

Query: 561 TLAVANDARLYAFNNGTQTVVISSLEVWNMSNAQIK 597
           TLAV N+ARLYAFNNGTQTVVISSL+ WNMSNA+IK
Sbjct: 541 TLAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEIK 575

BLAST of Bhi04G000459 vs. NCBI nr
Match: XP_011649529.2 (beta-fructofuranosidase, insoluble isoenzyme CWINV3 isoform X2 [Cucumis sativus])

HSP 1 Score: 1054.3 bits (2725), Expect = 4.0e-304
Identity = 502/578 (86.85%), Postives = 537/578 (92.91%), Query Frame = 0

Query: 19  DKMPTKCLQMGKCGLIALYFLLVISNRIGFVVSHEYANGVHIPQQIQRTSYHFQPLKNWM 78
           DKMP K L++GK GL+  + +L+  NRIGFVVSHEYAN VH P Q+QRTSYHFQPLKNWM
Sbjct: 31  DKMPPKRLEIGKYGLVVSFVILLTCNRIGFVVSHEYANEVHTP-QVQRTSYHFQPLKNWM 90

Query: 79  NDPNGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEPSDPFDING 138
              NGPMFYKGIYH FYQHNPNGAVFNSKMVWAHSIS+DLINWVHLNHALEP+DPFDING
Sbjct: 91  ---NGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDING 150

Query: 139 CWSGSVSFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEKWRKSSQNPIIAPPNGV 198
           CWSGSVSFL +NKP ILYTGIDSS QQVQNLAIP N+SDPFLEKWRK SQNPIIAPP+G+
Sbjct: 151 CWSGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIAPPDGL 210

Query: 199 ERDKFRDPTTAWQGPDGEWRVAVGGHTSYGGAAMLYRSEDFVHWHQFRFPLYSSRDSGTW 258
           ER++FRDPTTAWQGPDGEWRVA+GG TSYGGAAMLYRSEDFV WHQFRFPLYSS+DSGTW
Sbjct: 211 ERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTW 270

Query: 259 ECPDFYPVMLNGTNGIDFSSGFGFGVKYVMKASFNSHDYYTLGSYATEKEKFTSDSSSGF 318
           ECPDFYPVMLNGTNGI FSSGFG GVKYVMKASFNS D+YTLGSY  EKEKFTSD   GF
Sbjct: 271 ECPDFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTLGSYVPEKEKFTSDYGPGF 330

Query: 319 DFRGTNLGLRYDYGKFYASKTFYDSSKKRRILWGWVNESDSREDDIKKGWSGLQAVPRKI 378
           DF+G NLGLRYDYGKFYASKTFYDSS+KRRILWGWVNESDSR+DDI KGWSGLQA+PRKI
Sbjct: 331 DFKGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRKI 390

Query: 379 WLSKTGRQLTQWPVKEIEMLRRNHFSLHHKELRGRSTMEILGGSASQVDVEVSFESPNLE 438
           WLSKTGRQL QWPVKEI+MLRRNHFSLHHKELRGRSTME+LGGSASQVDVEVSFE P LE
Sbjct: 391 WLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPYLE 450

Query: 439 EAESVETRWDDLDPQLLCSKRDASVNGRIGPFGLLVLASNDLSEHTAIFFHILKAHNRYV 498
           EAESV+TRW  LDPQ+LCSKRDASVNGR+GPFGLLVLASNDLSEHTAI+FHILKAHNRYV
Sbjct: 451 EAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHNRYV 510

Query: 499 VLMCSDQSRSSFRKGVDKTTYGAFVDVDPCHKKISLRTLVDHTIVESFGGKGKTCITSRV 558
           VLMCSDQSRSSFRK VDKT +GAFVD+DPC+KKISLRTLVDH+IVESFGGKGKTCITSRV
Sbjct: 511 VLMCSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSRV 570

Query: 559 YPTLAVANDARLYAFNNGTQTVVISSLEVWNMSNAQIK 597
           YPTLAV N+ARLYAFNNGTQTVVISSL+ WNMSNA+IK
Sbjct: 571 YPTLAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEIK 604

BLAST of Bhi04G000459 vs. ExPASy TrEMBL
Match: A0A1S3BB78 (LOW QUALITY PROTEIN: beta-fructofuranosidase, insoluble isoenzyme CWINV3-like OS=Cucumis melo OX=3656 GN=LOC103487780 PE=3 SV=1)

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 511/579 (88.26%), Postives = 545/579 (94.13%), Query Frame = 0

Query: 19  DKMPTKCLQMGKCGLIALYFLLVISNRIGFVVSHEYANGVH-IPQQIQRTSYHFQPLKNW 78
           DKMP K L+MGK GL+A + LL+ SNR+GFVVSHEYAN VH  PQQ+QRTSYHFQPLKNW
Sbjct: 2   DKMPPKRLEMGKYGLVASFILLLASNRMGFVVSHEYANEVHSTPQQVQRTSYHFQPLKNW 61

Query: 79  MNDPNGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEPSDPFDIN 138
           MNDPNGPMFYKGIYH FYQHNPNGAVFNSKMVWAHSIS+DLINWVHLNHALEP+DPFDIN
Sbjct: 62  MNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDIN 121

Query: 139 GCWSGSVSFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEKWRKSSQNPIIAPPNG 198
           GCWSGSVSFL KNKP ILYTGIDSSFQQVQNLAIP N+SDPFLEKWRKS QNPIIAPP+G
Sbjct: 122 GCWSGSVSFLPKNKPRILYTGIDSSFQQVQNLAIPTNYSDPFLEKWRKSPQNPIIAPPDG 181

Query: 199 VERDKFRDPTTAWQGPDGEWRVAVGGHTSYGGAAMLYRSEDFVHWHQFRFPLYSSRDSGT 258
            ER++FRDPTTAWQGPDGEWRVA+GG TSYGGAAMLYRSEDFV WHQFRFPLYSS+DSGT
Sbjct: 182 AERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGT 241

Query: 259 WECPDFYPVMLNGTNGIDFSSGFGFGVKYVMKASFNSHDYYTLGSYATEKEKFTSDSSSG 318
           WECPDFYPVMLNGTNGI FSSGFG GVK+VMKASFNS D+YTLGSY  EKEKFTSD   G
Sbjct: 242 WECPDFYPVMLNGTNGIGFSSGFGVGVKHVMKASFNSRDHYTLGSYVPEKEKFTSDHGLG 301

Query: 319 FDFRGTNLGLRYDYGKFYASKTFYDSSKKRRILWGWVNESDSREDDIKKGWSGLQAVPRK 378
           FDFRG NLGLRYDYGKFYASKTFYDSS+KRRILWGWVNESDSR+DDI KGWSGLQA+PRK
Sbjct: 302 FDFRGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRK 361

Query: 379 IWLSKTGRQLTQWPVKEIEMLRRNHFSLHHKELRGRSTMEILGGSASQVDVEVSFESPNL 438
           IWLSKTGRQL QWPVKEI+MLRRNHFSLHHKELRGRSTME+LGGSASQVDVEVSF  P+L
Sbjct: 362 IWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFALPHL 421

Query: 439 EEAESVETRWDDLDPQLLCSKRDASVNGRIGPFGLLVLASNDLSEHTAIFFHILKAHNRY 498
           EEAESV+TRW  LDPQ+LCSKRDAS+NGR+GPFGLLVLASNDLSEHTAI+FH+LKAHNRY
Sbjct: 422 EEAESVDTRWGVLDPQVLCSKRDASMNGRVGPFGLLVLASNDLSEHTAIYFHVLKAHNRY 481

Query: 499 VVLMCSDQSRSSFRKGVDKTTYGAFVDVDPCHKKISLRTLVDHTIVESFGGKGKTCITSR 558
           VVLMCSD+SRSSFRKGVDKTTYGAFVD+DP +KKISLRTLVDH+IVESFGGKGKTCITSR
Sbjct: 482 VVLMCSDESRSSFRKGVDKTTYGAFVDIDPRYKKISLRTLVDHSIVESFGGKGKTCITSR 541

Query: 559 VYPTLAVANDARLYAFNNGTQTVVISSLEVWNMSNAQIK 597
           VYPTLAV NDARLYAFNNGTQTVVISSL+VWNMSNA+IK
Sbjct: 542 VYPTLAVNNDARLYAFNNGTQTVVISSLKVWNMSNAEIK 580

BLAST of Bhi04G000459 vs. ExPASy TrEMBL
Match: A0A0A0LQX1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G351670 PE=3 SV=1)

HSP 1 Score: 1067.0 bits (2758), Expect = 2.9e-308
Identity = 505/578 (87.37%), Postives = 540/578 (93.43%), Query Frame = 0

Query: 19  DKMPTKCLQMGKCGLIALYFLLVISNRIGFVVSHEYANGVHIPQQIQRTSYHFQPLKNWM 78
           DKMP K L++GK GL+  + +L+  NRIGFVVSHEYAN VH P Q+QRTSYHFQPLKNWM
Sbjct: 20  DKMPPKRLEIGKYGLVVSFVILLTCNRIGFVVSHEYANEVHTP-QVQRTSYHFQPLKNWM 79

Query: 79  NDPNGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEPSDPFDING 138
           NDPNGPMFYKGIYH FYQHNPNGAVFNSKMVWAHSIS+DLINWVHLNHALEP+DPFDING
Sbjct: 80  NDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDING 139

Query: 139 CWSGSVSFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEKWRKSSQNPIIAPPNGV 198
           CWSGSVSFL +NKP ILYTGIDSS QQVQNLAIP N+SDPFLEKWRK SQNPIIAPP+G+
Sbjct: 140 CWSGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIAPPDGL 199

Query: 199 ERDKFRDPTTAWQGPDGEWRVAVGGHTSYGGAAMLYRSEDFVHWHQFRFPLYSSRDSGTW 258
           ER++FRDPTTAWQGPDGEWRVA+GG TSYGGAAMLYRSEDFV WHQFRFPLYSS+DSGTW
Sbjct: 200 ERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTW 259

Query: 259 ECPDFYPVMLNGTNGIDFSSGFGFGVKYVMKASFNSHDYYTLGSYATEKEKFTSDSSSGF 318
           ECPDFYPVMLNGTNGI FSSGFG GVKYVMKASFNS D+YTLGSY  EKEKFTSD   GF
Sbjct: 260 ECPDFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTLGSYVPEKEKFTSDYGPGF 319

Query: 319 DFRGTNLGLRYDYGKFYASKTFYDSSKKRRILWGWVNESDSREDDIKKGWSGLQAVPRKI 378
           DF+G NLGLRYDYGKFYASKTFYDSS+KRRILWGWVNESDSR+DDI KGWSGLQA+PRKI
Sbjct: 320 DFKGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRKI 379

Query: 379 WLSKTGRQLTQWPVKEIEMLRRNHFSLHHKELRGRSTMEILGGSASQVDVEVSFESPNLE 438
           WLSKTGRQL QWPVKEI+MLRRNHFSLHHKELRGRSTME+LGGSASQVDVEVSFE P LE
Sbjct: 380 WLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPYLE 439

Query: 439 EAESVETRWDDLDPQLLCSKRDASVNGRIGPFGLLVLASNDLSEHTAIFFHILKAHNRYV 498
           EAESV+TRW  LDPQ+LCSKRDASVNGR+GPFGLLVLASNDLSEHTAI+FHILKAHNRYV
Sbjct: 440 EAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHNRYV 499

Query: 499 VLMCSDQSRSSFRKGVDKTTYGAFVDVDPCHKKISLRTLVDHTIVESFGGKGKTCITSRV 558
           VLMCSDQSRSSFRK VDKT +GAFVD+DPC+KKISLRTLVDH+IVESFGGKGKTCITSRV
Sbjct: 500 VLMCSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSRV 559

Query: 559 YPTLAVANDARLYAFNNGTQTVVISSLEVWNMSNAQIK 597
           YPTLAV N+ARLYAFNNGTQTVVISSL+ WNMSNA+IK
Sbjct: 560 YPTLAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEIK 596

BLAST of Bhi04G000459 vs. ExPASy TrEMBL
Match: A0A2I4JRG1 (Cell wall invertase 4 OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 1062.4 bits (2746), Expect = 7.0e-307
Identity = 503/576 (87.33%), Postives = 538/576 (93.40%), Query Frame = 0

Query: 21  MPTKCLQMGKCGLIALYFLLVISNRIGFVVSHEYANGVHIPQQIQRTSYHFQPLKNWMND 80
           MP K L++GK GL+  + +L+  NRIGFVVSHEYAN VH P Q+QRTSYHFQPLKNWMND
Sbjct: 1   MPPKRLEIGKYGLVVSFVILLTCNRIGFVVSHEYANEVHTP-QVQRTSYHFQPLKNWMND 60

Query: 81  PNGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEPSDPFDINGCW 140
           PNGPMFYKGIYH FYQHNPNGAVFNSKMVWAHSIS+DLINWVHLNHALEP+DPFDINGCW
Sbjct: 61  PNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNHALEPTDPFDINGCW 120

Query: 141 SGSVSFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEKWRKSSQNPIIAPPNGVER 200
           SGSVSFL +NKP ILYTGIDSS QQVQNLAIP N+SDPFLEKWRK SQNPIIAPP+G+ER
Sbjct: 121 SGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWRKFSQNPIIAPPDGLER 180

Query: 201 DKFRDPTTAWQGPDGEWRVAVGGHTSYGGAAMLYRSEDFVHWHQFRFPLYSSRDSGTWEC 260
           ++FRDPTTAWQGPDGEWRVA+GG TSYGGAAMLYRSEDFV WHQFRFPLYSS+DSGTWEC
Sbjct: 181 NRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFRFPLYSSQDSGTWEC 240

Query: 261 PDFYPVMLNGTNGIDFSSGFGFGVKYVMKASFNSHDYYTLGSYATEKEKFTSDSSSGFDF 320
           PDFYPVMLNGTNGI FSSGFG GVKYVMKASFNS D+YTLGSY  EKEKFTSD   GFDF
Sbjct: 241 PDFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTLGSYVPEKEKFTSDYGPGFDF 300

Query: 321 RGTNLGLRYDYGKFYASKTFYDSSKKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIWL 380
           +G NLGLRYDYGKFYASKTFYDSS+KRRILWGWVNESDSR+DDI KGWSGLQA+PRKIWL
Sbjct: 301 KGINLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSRQDDINKGWSGLQAIPRKIWL 360

Query: 381 SKTGRQLTQWPVKEIEMLRRNHFSLHHKELRGRSTMEILGGSASQVDVEVSFESPNLEEA 440
           SKTGRQL QWPVKEI+MLRRNHFSLHHKELRGRSTME+LGGSASQVDVEVSFE P LEEA
Sbjct: 361 SKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGGSASQVDVEVSFELPYLEEA 420

Query: 441 ESVETRWDDLDPQLLCSKRDASVNGRIGPFGLLVLASNDLSEHTAIFFHILKAHNRYVVL 500
           ESV+TRW  LDPQ+LCSKRDASVNGR+GPFGLLVLASNDLSEHTAI+FHILKAHNRYVVL
Sbjct: 421 ESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIYFHILKAHNRYVVL 480

Query: 501 MCSDQSRSSFRKGVDKTTYGAFVDVDPCHKKISLRTLVDHTIVESFGGKGKTCITSRVYP 560
           MCSDQSRSSFRK VDKT +GAFVD+DPC+KKISLRTLVDH+IVESFGGKGKTCITSRVYP
Sbjct: 481 MCSDQSRSSFRKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSRVYP 540

Query: 561 TLAVANDARLYAFNNGTQTVVISSLEVWNMSNAQIK 597
           TLAV N+ARLYAFNNGTQTVVISSL+ WNMSNA+IK
Sbjct: 541 TLAVNNNARLYAFNNGTQTVVISSLKAWNMSNAEIK 575

BLAST of Bhi04G000459 vs. ExPASy TrEMBL
Match: A0A6J1HDH1 (beta-fructofuranosidase, insoluble isoenzyme CWINV3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462607 PE=3 SV=1)

HSP 1 Score: 1021.5 bits (2640), Expect = 1.4e-294
Identity = 485/576 (84.20%), Postives = 522/576 (90.62%), Query Frame = 0

Query: 21  MPTKCLQMGKCGLIALYFLLVISNRIGFVVSHEYANGVHIPQQIQRTSYHFQPLKNWMND 80
           M TK ++M KCGL+ALYF+ +ISNR GFVVSH   N VHIPQQ QRTSYHFQPL+NWMND
Sbjct: 1   MATKWMEMVKCGLVALYFVWLISNRFGFVVSHGDINEVHIPQQFQRTSYHFQPLQNWMND 60

Query: 81  PNGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEPSDPFDINGCW 140
           PNGPM+YKGIYHLFYQHNPNGAVF+SK+VW H++S DLINW HLNHALEP+ PFDINGCW
Sbjct: 61  PNGPMYYKGIYHLFYQHNPNGAVFDSKIVWGHAVSRDLINWAHLNHALEPTHPFDINGCW 120

Query: 141 SGSVSFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEKWRKSSQNPIIAPPNGVER 200
           SGSVSFL  NKP+ILYTG+DSSFQQVQNLAIP NFSDPFLE W KSS NPIIAPPNG+ER
Sbjct: 121 SGSVSFLHNNKPVILYTGLDSSFQQVQNLAIPSNFSDPFLEIWTKSSHNPIIAPPNGIER 180

Query: 201 DKFRDPTTAWQGPDGEWRVAVGGHTSYGGAAMLYRSEDFVHWHQFRFPLYSSRDSGTWEC 260
            +FRDPTTAW GPDG WR+ +GG TSYGGAAMLYRSEDFVHWHQ RFPLYSSRDSG WEC
Sbjct: 181 HRFRDPTTAWPGPDGGWRIVIGGQTSYGGAAMLYRSEDFVHWHQSRFPLYSSRDSGNWEC 240

Query: 261 PDFYPVMLNGTNGIDFSSGFGFG-VKYVMKASFNSHDYYTLGSYATEKEKFTSDSSSGFD 320
           PDFYPV LNG+NGID  +GF  G VKYVMKASFNSHDYYTLGSY  EKEKFTSDS  GFD
Sbjct: 241 PDFYPVKLNGSNGIDSLNGFRVGAVKYVMKASFNSHDYYTLGSYVPEKEKFTSDSGDGFD 300

Query: 321 FRGTNLGLRYDYGKFYASKTFYDSSKKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIW 380
            RGTNLGLRYDYGKFYASKTFYDSS+KRRILWGWVNESDSRED+IKKGW+GLQ VPRKIW
Sbjct: 301 LRGTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDNIKKGWAGLQVVPRKIW 360

Query: 381 LSKTGRQLTQWPVKEIEMLRRNHFSLHHKELRGRSTMEILGGSASQVDVEVSFESPNLEE 440
           LSKTGRQL QWPVKE+E LR N FSL++KELRGRSTM++LGGSASQVD EVSFE P LEE
Sbjct: 361 LSKTGRQLIQWPVKEVEKLRTNRFSLYNKELRGRSTMKVLGGSASQVDAEVSFEFPYLEE 420

Query: 441 AESVETRWDDLDPQLLCSKRDASVNGRIGPFGLLVLASNDLSEHTAIFFHILKAHNRYVV 500
           AE V+TRW DLDPQLLCSKRDASVNGR+GPFGLLVLASNDLSEHTAIFF +LKAHNRYVV
Sbjct: 421 AERVDTRWFDLDPQLLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIFFRVLKAHNRYVV 480

Query: 501 LMCSDQSRSSFRKGVDKTTYGAFVDVDPCHKKISLRTLVDHTIVESFGGKGKTCITSRVY 560
           LMCSDQSRSSFR+GVDKTTYGAFVDVDP H+KISLRTLVD +IVESFGGKGKTCITSRVY
Sbjct: 481 LMCSDQSRSSFREGVDKTTYGAFVDVDPRHEKISLRTLVDRSIVESFGGKGKTCITSRVY 540

Query: 561 PTLAVANDARLYAFNNGTQTVVISSLEVWNMSNAQI 596
           PTLAV +DARLYAFNNGTQ+VVISSL+VW M NAQI
Sbjct: 541 PTLAVDDDARLYAFNNGTQSVVISSLKVWKMWNAQI 576

BLAST of Bhi04G000459 vs. ExPASy TrEMBL
Match: A0A6J1KAT6 (beta-fructofuranosidase, insoluble isoenzyme CWINV3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491703 PE=3 SV=1)

HSP 1 Score: 1019.6 bits (2635), Expect = 5.2e-294
Identity = 483/576 (83.85%), Postives = 521/576 (90.45%), Query Frame = 0

Query: 21  MPTKCLQMGKCGLIALYFLLVISNRIGFVVSHEYANGVHIPQQIQRTSYHFQPLKNWMND 80
           M TK L+M KCG++ALYFL +ISNR GFVVSH   N VHIPQQ QRTSYHF PLKNWMND
Sbjct: 1   MVTKGLEMVKCGVVALYFLWLISNRFGFVVSHGDINEVHIPQQFQRTSYHFHPLKNWMND 60

Query: 81  PNGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEPSDPFDINGCW 140
           PNGPM+YKGIYHLFYQHNPNGAVF+SK+VW H++S DLINW HLNHALEP+ PFDINGCW
Sbjct: 61  PNGPMYYKGIYHLFYQHNPNGAVFDSKIVWGHAVSRDLINWAHLNHALEPTHPFDINGCW 120

Query: 141 SGSVSFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEKWRKSSQNPIIAPPNGVER 200
           SGSVSFL  NKP+ILYTG+DSSFQQVQNLAIP NFSDPFLE W KSS NPII PPNGVER
Sbjct: 121 SGSVSFLHNNKPVILYTGLDSSFQQVQNLAIPSNFSDPFLEIWTKSSHNPIIVPPNGVER 180

Query: 201 DKFRDPTTAWQGPDGEWRVAVGGHTSYGGAAMLYRSEDFVHWHQFRFPLYSSRDSGTWEC 260
            +FRDPTTAW GPDG WR+A+GG TSYGGAAMLY SEDFVHW+Q RFPLYSSRDSG WEC
Sbjct: 181 HRFRDPTTAWPGPDGGWRIAIGGQTSYGGAAMLYGSEDFVHWYQSRFPLYSSRDSGNWEC 240

Query: 261 PDFYPVMLNGTNGIDFSSGFGFG-VKYVMKASFNSHDYYTLGSYATEKEKFTSDSSSGFD 320
           PDFYPV LNG+NG+DF +G   G VKYVMKAS NSHDYYTLGSY  EKEKFTSDS  GFD
Sbjct: 241 PDFYPVKLNGSNGVDFLNGSRVGVVKYVMKASLNSHDYYTLGSYVPEKEKFTSDSGDGFD 300

Query: 321 FRGTNLGLRYDYGKFYASKTFYDSSKKRRILWGWVNESDSREDDIKKGWSGLQAVPRKIW 380
            RGTNLGLRYDYGKFYASKTFYDSS+KRRILWGWVNESDSRED+IKKGW+GLQ +PRKIW
Sbjct: 301 LRGTNLGLRYDYGKFYASKTFYDSSQKRRILWGWVNESDSREDNIKKGWAGLQVIPRKIW 360

Query: 381 LSKTGRQLTQWPVKEIEMLRRNHFSLHHKELRGRSTMEILGGSASQVDVEVSFESPNLEE 440
           LSKTGRQL QWPVKE+E LR NHFSL++KELRGRSTM++LGGSASQVD EVSFE P LEE
Sbjct: 361 LSKTGRQLIQWPVKEVEKLRTNHFSLYNKELRGRSTMKVLGGSASQVDTEVSFEFPYLEE 420

Query: 441 AESVETRWDDLDPQLLCSKRDASVNGRIGPFGLLVLASNDLSEHTAIFFHILKAHNRYVV 500
           AE V+TRW DLDPQLLCSKRDASVNGR+GPFGLLVLASNDLSEHTAIFFH+LKAHNRYVV
Sbjct: 421 AERVDTRWFDLDPQLLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIFFHVLKAHNRYVV 480

Query: 501 LMCSDQSRSSFRKGVDKTTYGAFVDVDPCHKKISLRTLVDHTIVESFGGKGKTCITSRVY 560
           LMCSDQSRSSFR+GVDKTTYGAFVD+DP H+KISLRTLVD +IVESFG KGKTCITSRVY
Sbjct: 481 LMCSDQSRSSFREGVDKTTYGAFVDIDPRHEKISLRTLVDRSIVESFGAKGKTCITSRVY 540

Query: 561 PTLAVANDARLYAFNNGTQTVVISSLEVWNMSNAQI 596
           PTLAV +DARLYAFNNGTQ+VVISSL+VW MSNAQI
Sbjct: 541 PTLAVDDDARLYAFNNGTQSVVISSLKVWKMSNAQI 576