Bhi04G000456 (gene) Wax gourd (B227) v1

Overview
NameBhi04G000456
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionZinc transporter 4
Locationchr4: 12827905 .. 12831775 (-)
RNA-Seq ExpressionBhi04G000456
SyntenyBhi04G000456
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTGTTAGCAATGAAAAATGAGGGCATAAAGAGTGTCGACACTATCGACCTCATTCTGAAGCAAAAAGTCATAACTGTTTAGTTTATTCCTCTGTTTCTTCCTTTCATTTCCTTCCATTATGTCGACACTCTTCTTCTTCCCTTCCCACTTCCCCACTTTCATCTTCTTCCTTCTCTATCTCTTTATCTTTCTCTTTTTCTCTTTTTTTTCTTCTTCCTTCATCCATCCCTCTCTGTTCCAACTCTTTTTTAAAAACCCAAAACAACCCATCTTCTCTCCAAACGCCCCATCAATCTACTCCTTCTCTTCCTCTTTGCCTGATTCCGTTTCTGGCGCTTTCCCTGTTCGAAGATTCCAGATTTGGGTTCTTCGAATTTCTAATCGGGTTTTAATTCCCCACTCTACCTTCCCTCTTCTCTCATGTTTTTCTTTGAGGTTAGTTCCAACCCCTCCTCTCTTCTTTCCCCCAATTTCATTTTGCTCATCGTTCTTCTGGTTCTTTTCTGTTTCAGGATTTGTTGCAGATGATCTGTATTCGAGGTTTCCAGGATAAAACTCGGCTTCTCTCTGGTAATTTCCATCTGTGTCTAAGCTGCTTTGAATTGAACTGGGTCAATGTTGGAATTTTCTGGGTTCTTACTTAATTCTTGTAATTGGTTGATTGATTGATTTGTGATTTCTGGGTTTTAATTTTATTAGGTTTATTTGGGAAATTCTGTGAATGGATGAGATTTCTATTCTGGGGGTTTCTTTTTCATTTTTTTTTTCAATTCCTGATCGAAACAGAGTCTTTCATACGGACCGTATCCGAATCCATGGCCAATTCATCTTGTGGGGTCTCAGAAGTAGAGCTCTGTAGAAACGATACGGCTGCGCTCCGTTACAAATTAATCGCCATCGCTTCGATTCTAACATCGGGAGTCATCGGAATCGCCCTTCCATTGTTGGGTAGACAGTGTCGGTTCCTCAAAACAGACGGCAACCTGTTCGTGGCAGCGAAGGCGTTTGCCGCCGGTGTGATTCTAGCCACCGCCTTCGTCCATATGCTTCCGGATGGATCCGACGCCCTCTCCGATCCGTGCTTGCCGGAATTTCCATGGTCCAAATTCCCATTCTCTGGATTCTTCGCTATGATGGCGTCGCTGTTGACGCTTCTCGTCGATTTCGTTGGAACTCAGTATTACGAGCGGAAACAGGGGATGATTCGGTCGAAAGAGGAGGCGAATAGGGTCGGCTCGGTGGAGGATTCCGGATTAGAATCCGGCATTGTTCCAGCCGGAGATTTGAACGTGAAACTATTTGGGGAAGAAGACGGCGGCGCGATGCACATCGTCGGAATGCATGCCCACGCCGCCCAGCACCGGCACAGTCATCCGCAAGGGAAGGAGGCATGTGATGGGCACGTGACGGTTCATGATCATGGGCATGGGCATGCCCATGGACATGGTCATTCTCATGGGCTGGACGGCGATGATGACGAAAGCGGTGTACGACATGTCGTTGTTTCCCAGGTAACTTTTTTTTTCTTCGAGTTTTCGCCCTCCACGTGTCAATGTCGCTTAAATTACTGACTAACGTTTTTTCCCTCTTGCCTTCTGGGGAACGTAATTTTGTTTGTCAAAGTCAACCATCCGAATTTGGTTCTTTTTTCATTTTGAAGAAAAAATTGTTTCAGTATATATATATCATTTAAAAAAAAAAAAATTAAGCTCATGAGTAGTTAAATTATTAATTTTAAGGTTGATAATTATTTTATTTTGATTTCTAAACTTTCAATATCTATTATTTCTAATAGATATTAAATTAGTTAGATGATGAATTTAATATAATTTAAATTTAAATATTTATTAGACATCAAATTGAAAGTAAATTTATTATACACTTTTTTATTGCATTATTTTTTTTACAACCAATTGTTATTGTTCATGGATCTTTTAAAGTTCAGTAATTAGTAATTTATTACATAGTTAATATTTTTTTTTTTTTTAAAAAAAAAAAAGTCATGATCCATTAGAAACAAAATAAAAAAAATTTATGATTTCATCACACACACAAAAGTAAAATTTTTGTCTATAGTTTAATTAGTTTATTAAATTTATTAGACACCTTTAAAAATTAAGTACTTATTGAGTACATTTATTAAACACTTTTTGTAATTTCAATTTTGTTTTGAAAGTTTAAAGATAAAAATATATTAAATAATTATTTCATCTGAACCTATTAGATAATATACTTTAGTCACCTTTTTTCCCCCTTTTTTTAGAATGATACTTTAGGGACCTGTATTAGACATTTTCAAACTTATAAGACCCTTTTTGAGAATGTTTTTCTTTTAATTATTCAATATACTTTTGAAATTTTAAATTAGAAACAATGGCCTATTAGGTACAATCCTCAAACTTAGTGAATTTTTCAGATTACAAAGTTAGTTTACTTGGTGGGTAAGGTTATAAACACAAAATTCAAGTGAGGTTTTGAAAATTAAATTTAGAATTTTTTTTAAAAACAAAATAGGTATCAAATACTATTTTAATGAATTGTTAAAATCTTTCTCAAAACCACATCTCTTTAGTTGTTGTATACTTTTATTGTTGTTGTTTTAAACTTGATAAAATTGTAAAAGGAAGATGTTGAGAGTTGAATTATGATTTTTTTTTAAAAAAAAAAAATTAGTGATGTTTTCTATATTTGAATAATAATTTTGAAATTCTAGTCAAATCTCAAAAAGATAATAAAAGTAGGGTCCATTTGATGATCTAATATATGATTTACTGAAAAAAGGACAAATTTTGAGAACAAACAACCAGAACATACAACAAGACAACAACAAAACACAATACTTCATATAAATGACAATGCATTATTGTAATATAAAGAATCCAATATTTATCTAAATGTTCAAAAATATATTTTCTTGAGCAGATATTGGAGCTTGGAATAGTGTCACATTCAGTGATAATTGGGCTGTCTCTGGGAGTGTCCCACAGCCCATGCACCATTAGACCTTTAATTGCAGCCCTGTCCTTCCATCAGTTCTTTGAAGGCTTTGCCCTTGGTGGCTGCATTTCTCAGGCTCAGTTCAACACCCTCTCCACCTCCCTCATGGCTGTCTTCTTTGCCATCACCACCCCCCTCGGGATCGCCGTAGGCGCTGCAGTTGCCTCTACCTACAACCCCAACAGCACCGGGGCGTTGGTTGCTGAAGGCATTTTGGACTCTCTATCGGCCGGAATTCTCGTGTACATGGCTCTTGTTGATTTGATTGCGGCCGACTTTCTTAACAAGAGAATGAGCTGCAATTTTAGGCTCCAAATTGTTTCGTATTGTACTCTCTTCCTCGGCGCGGGATTGATGTCTTCGCTCGCGCTTTGGGCTTGACCTCAAATTCAATTACATTACACAACTGTTTTTTTTTTTTTTTTTCTTTTCTAAAATTGGGATGAAATTGTTTCTTAAAAGCAAGAGGTAGATGATGTTAGGGAATTAATGCTTTTCACTTTTCAGAAGGGTTTGGAAGAACCCAACGCATTAGATTAGATTATGTATACTTATACTTAAAAATCTCTTCTGTAGCTTTACGTACCCCCCAAGAAAAAGAAAACAAGTTTGATGATGATATTGATTTTAGTTTTCCTTTTAATAACAATATTGTTCTGTAATTATATATATATTTATAAGTCACTCAATTTGAATTGCATCATTTTGGTACTAGAGTAATCCCAAAATGGCCCTGACATAGTATGACGAAGAAGTGGACTCTGAAGAGGTAAGTTGTATGTATGGAGTACGTGAATATAACCTCTAATTATTGATCATTTGATGTTTTTTCTCAAATTTTACAATTTGTTCTAATGTTGATTTATTTTCGGG

mRNA sequence

GTTGTTAGCAATGAAAAATGAGGGCATAAAGAGTGTCGACACTATCGACCTCATTCTGAAGCAAAAAGTCATAACTGTTTAGTTTATTCCTCTGTTTCTTCCTTTCATTTCCTTCCATTATGTCGACACTCTTCTTCTTCCCTTCCCACTTCCCCACTTTCATCTTCTTCCTTCTCTATCTCTTTATCTTTCTCTTTTTCTCTTTTTTTTCTTCTTCCTTCATCCATCCCTCTCTGTTCCAACTCTTTTTTAAAAACCCAAAACAACCCATCTTCTCTCCAAACGCCCCATCAATCTACTCCTTCTCTTCCTCTTTGCCTGATTCCGTTTCTGGCGCTTTCCCTGTTCGAAGATTCCAGATTTGGGTTCTTCGAATTTCTAATCGGGTTTTAATTCCCCACTCTACCTTCCCTCTTCTCTCATGTTTTTCTTTGAGGATTTGTTGCAGATGATCTGTATTCGAGGTTTCCAGGATAAAACTCGGCTTCTCTCTGAGTCTTTCATACGGACCGTATCCGAATCCATGGCCAATTCATCTTGTGGGGTCTCAGAAGTAGAGCTCTGTAGAAACGATACGGCTGCGCTCCGTTACAAATTAATCGCCATCGCTTCGATTCTAACATCGGGAGTCATCGGAATCGCCCTTCCATTGTTGGGTAGACAGTGTCGGTTCCTCAAAACAGACGGCAACCTGTTCGTGGCAGCGAAGGCGTTTGCCGCCGGTGTGATTCTAGCCACCGCCTTCGTCCATATGCTTCCGGATGGATCCGACGCCCTCTCCGATCCGTGCTTGCCGGAATTTCCATGGTCCAAATTCCCATTCTCTGGATTCTTCGCTATGATGGCGTCGCTGTTGACGCTTCTCGTCGATTTCGTTGGAACTCAGTATTACGAGCGGAAACAGGGGATGATTCGGTCGAAAGAGGAGGCGAATAGGGTCGGCTCGGTGGAGGATTCCGGATTAGAATCCGGCATTGTTCCAGCCGGAGATTTGAACGTGAAACTATTTGGGGAAGAAGACGGCGGCGCGATGCACATCGTCGGAATGCATGCCCACGCCGCCCAGCACCGGCACAGTCATCCGCAAGGGAAGGAGGCATGTGATGGGCACGTGACGGTTCATGATCATGGGCATGGGCATGCCCATGGACATGGTCATTCTCATGGGCTGGACGGCGATGATGACGAAAGCGGTGTACGACATGTCGTTGTTTCCCAGATATTGGAGCTTGGAATAGTGTCACATTCAGTGATAATTGGGCTGTCTCTGGGAGTGTCCCACAGCCCATGCACCATTAGACCTTTAATTGCAGCCCTGTCCTTCCATCAGTTCTTTGAAGGCTTTGCCCTTGGTGGCTGCATTTCTCAGGCTCAGTTCAACACCCTCTCCACCTCCCTCATGGCTGTCTTCTTTGCCATCACCACCCCCCTCGGGATCGCCGTAGGCGCTGCAGTTGCCTCTACCTACAACCCCAACAGCACCGGGGCGTTGGTTGCTGAAGGCATTTTGGACTCTCTATCGGCCGGAATTCTCGTGTACATGGCTCTTGTTGATTTGATTGCGGCCGACTTTCTTAACAAGAGAATGAGCTGCAATTTTAGGCTCCAAATTGTTTCGTATTGTACTCTCTTCCTCGGCGCGGGATTGATGTCTTCGCTCGCGCTTTGGGCTTGACCTCAAATTCAATTACATTACACAACTGTTTTTTTTTTTTTTTTTCTTTTCTAAAATTGGGATGAAATTGTTTCTTAAAAGCAAGAGGTAGATGATGTTAGGGAATTAATGCTTTTCACTTTTCAGAAGGGTTTGGAAGAACCCAACGCATTAGATTAGATTATGTATACTTATACTTAAAAATCTCTTCTGTAGCTTTACGTACCCCCCAAGAAAAAGAAAACAAGTTTGATGATGATATTGATTTTAGTTTTCCTTTTAATAACAATATTGTTCTGTAATTATATATATATTTATAAGTCACTCAATTTGAATTGCATCATTTTGGTACTAGAGTAATCCCAAAATGGCCCTGACATAGTATGACGAAGAAGTGGACTCTGAAGAGGTAAGTTGTATGTATGGAGTACGTGAATATAACCTCTAATTATTGATCATTTGATGTTTTTTCTCAAATTTTACAATTTGTTCTAATGTTGATTTATTTTCGGG

Coding sequence (CDS)

ATGTTTTTCTTTGAGGATTTGTTGCAGATGATCTGTATTCGAGGTTTCCAGGATAAAACTCGGCTTCTCTCTGAGTCTTTCATACGGACCGTATCCGAATCCATGGCCAATTCATCTTGTGGGGTCTCAGAAGTAGAGCTCTGTAGAAACGATACGGCTGCGCTCCGTTACAAATTAATCGCCATCGCTTCGATTCTAACATCGGGAGTCATCGGAATCGCCCTTCCATTGTTGGGTAGACAGTGTCGGTTCCTCAAAACAGACGGCAACCTGTTCGTGGCAGCGAAGGCGTTTGCCGCCGGTGTGATTCTAGCCACCGCCTTCGTCCATATGCTTCCGGATGGATCCGACGCCCTCTCCGATCCGTGCTTGCCGGAATTTCCATGGTCCAAATTCCCATTCTCTGGATTCTTCGCTATGATGGCGTCGCTGTTGACGCTTCTCGTCGATTTCGTTGGAACTCAGTATTACGAGCGGAAACAGGGGATGATTCGGTCGAAAGAGGAGGCGAATAGGGTCGGCTCGGTGGAGGATTCCGGATTAGAATCCGGCATTGTTCCAGCCGGAGATTTGAACGTGAAACTATTTGGGGAAGAAGACGGCGGCGCGATGCACATCGTCGGAATGCATGCCCACGCCGCCCAGCACCGGCACAGTCATCCGCAAGGGAAGGAGGCATGTGATGGGCACGTGACGGTTCATGATCATGGGCATGGGCATGCCCATGGACATGGTCATTCTCATGGGCTGGACGGCGATGATGACGAAAGCGGTGTACGACATGTCGTTGTTTCCCAGATATTGGAGCTTGGAATAGTGTCACATTCAGTGATAATTGGGCTGTCTCTGGGAGTGTCCCACAGCCCATGCACCATTAGACCTTTAATTGCAGCCCTGTCCTTCCATCAGTTCTTTGAAGGCTTTGCCCTTGGTGGCTGCATTTCTCAGGCTCAGTTCAACACCCTCTCCACCTCCCTCATGGCTGTCTTCTTTGCCATCACCACCCCCCTCGGGATCGCCGTAGGCGCTGCAGTTGCCTCTACCTACAACCCCAACAGCACCGGGGCGTTGGTTGCTGAAGGCATTTTGGACTCTCTATCGGCCGGAATTCTCGTGTACATGGCTCTTGTTGATTTGATTGCGGCCGACTTTCTTAACAAGAGAATGAGCTGCAATTTTAGGCTCCAAATTGTTTCGTATTGTACTCTCTTCCTCGGCGCGGGATTGATGTCTTCGCTCGCGCTTTGGGCTTGA

Protein sequence

MFFFEDLLQMICIRGFQDKTRLLSESFIRTVSESMANSSCGVSEVELCRNDTAALRYKLIAIASILTSGVIGIALPLLGRQCRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSDALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVGSVEDSGLESGIVPAGDLNVKLFGEEDGGAMHIVGMHAHAAQHRHSHPQGKEACDGHVTVHDHGHGHAHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLNKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA
Homology
BLAST of Bhi04G000456 vs. TAIR 10
Match: AT1G60960.1 (iron regulated transporter 3 )

HSP 1 Score: 514.6 bits (1324), Expect = 7.4e-146
Identity = 275/428 (64.25%), Postives = 331/428 (77.34%), Query Frame = 0

Query: 1   MFFFEDLLQMICIRGFQDKTRLLS--ESFIRTVSESMANSS----CGVSEVELCRNDTAA 60
           MFF + L +++ +  F  +T+ LS  ES ++ V E+MA +S    C  SE +LCR+D+AA
Sbjct: 1   MFFVDVLWKLVPLYLFGSETKSLSATESILQIVPEAMAATSSNVLCNASESDLCRDDSAA 60

Query: 61  LRYKLIAIASILTSGVIGIALPLLGRQCRFLKTDGNLFVAAKAFAAGVILATAFVHMLPD 120
              K +AIASIL +G  G+ +PL+GR  RFL+TDGNLFV AKAFAAGVILAT FVHML  
Sbjct: 61  FLLKFVAIASILLAGAAGVTIPLIGRNRRFLQTDGNLFVTAKAFAAGVILATGFVHMLAG 120

Query: 121 GSDALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVG 180
           G++AL +PCLP+FPWSKFPF GFFAM+A+L+TL VDF+GTQYYERKQ    + E     G
Sbjct: 121 GTEALKNPCLPDFPWSKFPFPGFFAMIAALITLFVDFMGTQYYERKQER-EASESVEPFG 180

Query: 181 SVEDSGLESGIVPAGDLNVKLFGEEDGGAMHIVGMHAHAAQHRHSHPQGKEACDGHVTV- 240
             +  G+   ++  G  + K+FGEED G +HIVG+HAHAA HRHSHP G ++C+GH  + 
Sbjct: 181 REQSPGIVVPMIGEGTNDGKVFGEEDSGGIHIVGIHAHAAHHRHSHPPGHDSCEGHSKID 240

Query: 241 ----HDHGHGHAHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSP 300
               H HGHGH HGHGH HG  G D  +G RH+VVSQ+LELGIVSHS+IIGLSLGVS SP
Sbjct: 241 IGHAHAHGHGHGHGHGHVHG--GLDAVNGARHIVVSQVLELGIVSHSIIIGLSLGVSQSP 300

Query: 301 CTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTY 360
           CTIRPLIAALSFHQFFEGFALGGCISQAQF   S ++MA FFA+TTP+GI +G AVAS++
Sbjct: 301 CTIRPLIAALSFHQFFEGFALGGCISQAQFRNKSATIMACFFALTTPIGIGIGTAVASSF 360

Query: 361 NPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLNKRMSCNFRLQIVSYCTLFLGAGL 418
           N +S GALV EGILDSLSAGILVYMALVDLIAADFL+ +M CNFRLQIVSY  LFLGAGL
Sbjct: 361 NSHSVGALVTEGILDSLSAGILVYMALVDLIAADFLSTKMRCNFRLQIVSYVMLFLGAGL 420

BLAST of Bhi04G000456 vs. TAIR 10
Match: AT1G10970.1 (zinc transporter 4 precursor )

HSP 1 Score: 495.7 bits (1275), Expect = 3.5e-140
Identity = 274/425 (64.47%), Postives = 322/425 (75.76%), Query Frame = 0

Query: 1   MFFFEDLLQMICIRGFQDKTRLLSESFIRTVSESMANSS----CGVSEVELCRNDTAALR 60
           M F + L ++  +  F      LSES ++ + E+MA+S+    C   E +LCR+D+AA  
Sbjct: 1   MIFVDVLWKLFPLYSFGSGRDSLSESILQIIPETMASSTTKILCDAGESDLCRDDSAAFL 60

Query: 61  YKLIAIASILTSGVIGIALPLLGRQCRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGS 120
            K +AIASIL +G  G+A+PL+GR  RFL+T+GNLFVAAKAFAAGVILAT FVHML  G+
Sbjct: 61  LKFVAIASILLAGAAGVAIPLIGRNRRFLQTEGNLFVAAKAFAAGVILATGFVHMLAGGT 120

Query: 121 DALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVGSV 180
           +ALS+PCLP+FPWSKFPF GFFAM+A+L TLLVDF+GTQYYERKQ       E N+  + 
Sbjct: 121 EALSNPCLPDFPWSKFPFPGFFAMVAALATLLVDFMGTQYYERKQ-------ERNQAATE 180

Query: 181 EDSGLES-GIVPAGDLNV---KLFGEEDGGAMHIVGMHAHAAQHRHSHPQGKEACDGHVT 240
             +G E   +VP     V   K+FGEEDGG +HIVG+ AHAA HRHSH      CDGH  
Sbjct: 181 AAAGSEEIAVVPVVGERVTDNKVFGEEDGGGIHIVGIRAHAAHHRHSHSNSHGTCDGHA- 240

Query: 241 VHDHGHGHAHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTI 300
              HGH H H HG+S      D E+G RHVVVSQILELGIVSHS+IIGLSLGVS SPCTI
Sbjct: 241 ---HGHSHGHMHGNS------DVENGARHVVVSQILELGIVSHSIIIGLSLGVSQSPCTI 300

Query: 301 RPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPN 360
           RPLIAALSFHQFFEGFALGGCISQAQF   S ++MA FFA+TTPLGI +G AVAS++N +
Sbjct: 301 RPLIAALSFHQFFEGFALGGCISQAQFRNKSATIMACFFALTTPLGIGIGTAVASSFNSH 360

Query: 361 STGALVAEGILDSLSAGILVYMALVDLIAADFLNKRMSCNFRLQIVSYCTLFLGAGLMSS 418
           S GALV EGILDSLSAGILVYMALVDLIAADFL+KRMSCN RLQ+VSY  LFLGAGLMS+
Sbjct: 361 SPGALVTEGILDSLSAGILVYMALVDLIAADFLSKRMSCNLRLQVVSYVMLFLGAGLMSA 408

BLAST of Bhi04G000456 vs. TAIR 10
Match: AT4G33020.1 (ZIP metal ion transporter family )

HSP 1 Score: 409.5 bits (1051), Expect = 3.3e-114
Identity = 233/362 (64.36%), Postives = 273/362 (75.41%), Query Frame = 0

Query: 62  IASILTSGVIGIALPLLGRQCRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSDALSD 121
           +ASIL SG  G+++PL+G     L  +G L   AKAFAAGVILAT FVHML  GS ALSD
Sbjct: 1   MASILISGAAGVSIPLVG---TLLPLNGGLMRGAKAFAAGVILATGFVHMLSGGSKALSD 60

Query: 122 PCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG--MIRSKEE-ANRVGSVED 181
           PCLPEFPW  FPF  FFAM+A+LLTLL DF+ T YYERKQ   M +S E    +V  + D
Sbjct: 61  PCLPEFPWKMFPFPEFFAMVAALLTLLADFMITGYYERKQEKMMNQSVESLGTQVSVMSD 120

Query: 182 SGLESGIVPAGDLNVKLFGEEDGGAMHIVGMHAHAAQHRHS---HPQGKEACDGHVTVHD 241
            GLESG          L  +EDGGA+HIVGM AHA  HRHS     +G EA      V  
Sbjct: 121 PGLESGF---------LRDQEDGGALHIVGMRAHAEHHRHSLSMGAEGFEALSKRSGVSG 180

Query: 242 HGHGHAHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPL 301
           HGHGH+HGHG   GLD     SGVRHVVVSQILE+GIVSHS+IIG+SLGVSHSPCTIRPL
Sbjct: 181 HGHGHSHGHG-DVGLD-----SGVRHVVVSQILEMGIVSHSIIIGISLGVSHSPCTIRPL 240

Query: 302 IAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTG 361
           + ALSFHQFFEGFALGGC+++A+     +++MA FFAITTP+G+AVG A+AS+YN  S  
Sbjct: 241 LLALSFHQFFEGFALGGCVAEARLTPRGSAMMAFFFAITTPIGVAVGTAIASSYNSYSVA 300

Query: 362 ALVAEGILDSLSAGILVYMALVDLIAADFLNKRMSCNFRLQIVSYCTLFLGAGLMSSLAL 418
           ALVAEG+LDSLSAGILVYMALVDLIAADFL+K+MS +FR+Q+VSYC LFLGAG+MS+LA+
Sbjct: 301 ALVAEGVLDSLSAGILVYMALVDLIAADFLSKKMSVDFRVQVVSYCFLFLGAGMMSALAI 344

BLAST of Bhi04G000456 vs. TAIR 10
Match: AT1G05300.1 (zinc transporter 5 precursor )

HSP 1 Score: 278.5 bits (711), Expect = 8.9e-75
Identity = 166/391 (42.46%), Postives = 235/391 (60.10%), Query Frame = 0

Query: 31  VSESMANSSCGVSEVELCRNDTAALRYKLIAIASILTSGVIGIALPLLGRQCRFLKTDGN 90
           ++ S   S C  S  +   N   A +YK+ AI S+L +GVIG+  PLLG+    LK +  
Sbjct: 22  IAVSAGESKCECSHEDDEANKAGAKKYKIAAIPSVLAAGVIGVMFPLLGKFFPSLKPETT 81

Query: 91  LFVAAKAFAAGVILATAFVHMLPDGSDALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVD 150
            F   KAFAAGVILAT F+H+LP+G + L+ PCL    W +FPF+GF AM+A++LTL VD
Sbjct: 82  FFFVTKAFAAGVILATGFMHVLPEGYEKLTSPCLKGEAW-EFPFTGFIAMVAAILTLSVD 141

Query: 151 FVGTQYYERKQGMIRSKEEANRVGSVEDSGLESGIVPAGDLNVKLFGEEDGGAMHIVGMH 210
              T Y+ +        + + R+G  E+     G                GG    +G+H
Sbjct: 142 SFATSYFHKAH-----FKTSKRIGDGEEQDAGGG----------------GGGGDELGLH 201

Query: 211 AHAAQHRHSHPQGKEACDGHVTVHDHGHGHAHGHGHSHGLDG-DDDESGV---RHVVVSQ 270
            HA                              HGH+HG+ G +  ES V   R  VV+Q
Sbjct: 202 VHA------------------------------HGHTHGIVGVESGESQVQLHRTRVVAQ 261

Query: 271 ILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSL 330
           +LE+GI+ HSV+IG+SLG S SP T + L AAL FHQ FEG  LGGCI+Q  FN +S ++
Sbjct: 262 VLEVGIIVHSVVIGISLGASQSPDTAKALFAALMFHQCFEGLGLGGCIAQGNFNCMSITI 321

Query: 331 MAVFFAITTPLGIAVGAAVASTYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLN 390
           M++FF++TTP+GIAVG A++S+Y+ +S  AL+ +G+L++ SAGIL+YM+LVD +AADF++
Sbjct: 322 MSIFFSVTTPVGIAVGMAISSSYDDSSPTALIVQGVLNAASAGILIYMSLVDFLAADFMH 360

Query: 391 KRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 418
            +M  N RLQI+++ +L +GAG+MS LA WA
Sbjct: 382 PKMQSNTRLQIMAHISLLVGAGVMSLLAKWA 360

BLAST of Bhi04G000456 vs. TAIR 10
Match: AT3G12750.1 (zinc transporter 1 precursor )

HSP 1 Score: 261.9 bits (668), Expect = 8.6e-70
Identity = 163/385 (42.34%), Postives = 229/385 (59.48%), Query Frame = 0

Query: 36  ANSSCGVSEVELCRNDT-AALRYKLIAIASILTSGVIGIALPLLGRQCRFLKTDGNLFVA 95
           A+S C   +  + +++   A + KL +IA +L +G +G++LPL+G++   L+ + ++F  
Sbjct: 28  ASSDCTSHDDPVSQDEAEKATKLKLGSIALLLVAGGVGVSLPLIGKRIPALQPENDIFFM 87

Query: 96  AKAFAAGVILATAFVHMLPDGSDALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGT 155
            KAFAAGVIL T FVH+LPD  + LS PCL +    KFPF+GF AM++++ TL++D   T
Sbjct: 88  VKAFAAGVILCTGFVHILPDAFERLSSPCLEDTTAGKFPFAGFVAMLSAMGTLMIDTFAT 147

Query: 156 QYYERKQGMIRSKEEANRVGSVEDSGLESGIVPAGDLNVKLFGEEDGGAMHIVGMHAHAA 215
            YY+R       +  +N  GS +             +NV +  EE  G +HI   H HA+
Sbjct: 148 GYYKR-------QHFSNNHGSKQ-------------VNVVVDEEEHAGHVHI---HTHAS 207

Query: 216 QHRHSHPQGKEACDGHVTVHDHGHGHAHGHGHSHGLDGDDDESGVRHVVVSQILELGIVS 275
                                        HGH+HG         +R  +VSQ+LE+GIV 
Sbjct: 208 -----------------------------HGHTHG-----STELIRRRIVSQVLEIGIVV 267

Query: 276 HSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAIT 335
           HSVIIG+SLG S S  TI+PL+AALSFHQFFEG  LGGCIS A   + ST LMA FF++T
Sbjct: 268 HSVIIGISLGASQSIDTIKPLMAALSFHQFFEGLGLGGCISLADMKSKSTVLMATFFSVT 327

Query: 336 TPLGIAVGAAVAS--TYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLNKRMSCN 395
            PLGI +G  ++S   Y   S  A++ EG+L++ SAGIL+YM+LVDL+A DF+N R+  N
Sbjct: 328 APLGIGIGLGMSSGLGYRKESKEAIMVEGMLNAASAGILIYMSLVDLLATDFMNPRLQSN 355

Query: 396 FRLQIVSYCTLFLGAGLMSSLALWA 418
             L + +Y +L LGAG MS LA+WA
Sbjct: 388 LWLHLAAYLSLVLGAGSMSLLAIWA 355

BLAST of Bhi04G000456 vs. ExPASy Swiss-Prot
Match: Q8LE59 (Fe(2+) transport protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IRT3 PE=2 SV=3)

HSP 1 Score: 514.6 bits (1324), Expect = 1.0e-144
Identity = 275/428 (64.25%), Postives = 331/428 (77.34%), Query Frame = 0

Query: 1   MFFFEDLLQMICIRGFQDKTRLLS--ESFIRTVSESMANSS----CGVSEVELCRNDTAA 60
           MFF + L +++ +  F  +T+ LS  ES ++ V E+MA +S    C  SE +LCR+D+AA
Sbjct: 1   MFFVDVLWKLVPLYLFGSETKSLSATESILQIVPEAMAATSSNVLCNASESDLCRDDSAA 60

Query: 61  LRYKLIAIASILTSGVIGIALPLLGRQCRFLKTDGNLFVAAKAFAAGVILATAFVHMLPD 120
              K +AIASIL +G  G+ +PL+GR  RFL+TDGNLFV AKAFAAGVILAT FVHML  
Sbjct: 61  FLLKFVAIASILLAGAAGVTIPLIGRNRRFLQTDGNLFVTAKAFAAGVILATGFVHMLAG 120

Query: 121 GSDALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVG 180
           G++AL +PCLP+FPWSKFPF GFFAM+A+L+TL VDF+GTQYYERKQ    + E     G
Sbjct: 121 GTEALKNPCLPDFPWSKFPFPGFFAMIAALITLFVDFMGTQYYERKQER-EASESVEPFG 180

Query: 181 SVEDSGLESGIVPAGDLNVKLFGEEDGGAMHIVGMHAHAAQHRHSHPQGKEACDGHVTV- 240
             +  G+   ++  G  + K+FGEED G +HIVG+HAHAA HRHSHP G ++C+GH  + 
Sbjct: 181 REQSPGIVVPMIGEGTNDGKVFGEEDSGGIHIVGIHAHAAHHRHSHPPGHDSCEGHSKID 240

Query: 241 ----HDHGHGHAHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSP 300
               H HGHGH HGHGH HG  G D  +G RH+VVSQ+LELGIVSHS+IIGLSLGVS SP
Sbjct: 241 IGHAHAHGHGHGHGHGHVHG--GLDAVNGARHIVVSQVLELGIVSHSIIIGLSLGVSQSP 300

Query: 301 CTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTY 360
           CTIRPLIAALSFHQFFEGFALGGCISQAQF   S ++MA FFA+TTP+GI +G AVAS++
Sbjct: 301 CTIRPLIAALSFHQFFEGFALGGCISQAQFRNKSATIMACFFALTTPIGIGIGTAVASSF 360

Query: 361 NPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLNKRMSCNFRLQIVSYCTLFLGAGL 418
           N +S GALV EGILDSLSAGILVYMALVDLIAADFL+ +M CNFRLQIVSY  LFLGAGL
Sbjct: 361 NSHSVGALVTEGILDSLSAGILVYMALVDLIAADFLSTKMRCNFRLQIVSYVMLFLGAGL 420

BLAST of Bhi04G000456 vs. ExPASy Swiss-Prot
Match: O04089 (Zinc transporter 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ZIP4 PE=2 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 1.5e-135
Identity = 263/391 (67.26%), Postives = 303/391 (77.49%), Query Frame = 0

Query: 31  VSESMANSSCGVSEVELCRNDTAALRYKLIAIASILTSGVIGIALPLLGRQCRFLKTDGN 90
           ++ S     C   E +LCR+D+AA   K +AIASIL +G  G+A+PL+GR  RFL+T+GN
Sbjct: 1   MASSTTKILCDAGESDLCRDDSAAFLLKFVAIASILLAGAAGVAIPLIGRNRRFLQTEGN 60

Query: 91  LFVAAKAFAAGVILATAFVHMLPDGSDALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVD 150
           LFVAAKAFAAGVILAT FVHML  G++ALS+PCLP+FPWSKFPF GFFAM+A+L TLLVD
Sbjct: 61  LFVAAKAFAAGVILATGFVHMLAGGTEALSNPCLPDFPWSKFPFPGFFAMVAALATLLVD 120

Query: 151 FVGTQYYERKQGMIRSKEEANRVGSVEDSGLES-GIVPAGDLNV---KLFGEEDGGAMHI 210
           F+GTQYYERKQ       E N+  +   +G E   +VP     V   K+FGEEDGG +HI
Sbjct: 121 FMGTQYYERKQ-------ERNQAATEAAAGSEEIAVVPVVGERVTDNKVFGEEDGGGIHI 180

Query: 211 VGMHAHAAQHRHSHPQGKEACDGHVTVHDHGHGHAHGHGHSHGLDGDDDESGVRHVVVSQ 270
           VG+ AHAA HRHSH      CDGH     HGH H H HG+S      D E+G RHVVVSQ
Sbjct: 181 VGIRAHAAHHRHSHSNSHGTCDGHA----HGHSHGHMHGNS------DVENGARHVVVSQ 240

Query: 271 ILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSL 330
           ILELGIVSHS+IIGLSLGVS SPCTIRPLIAALSFHQFFEGFALGGCISQAQF   S ++
Sbjct: 241 ILELGIVSHSIIIGLSLGVSQSPCTIRPLIAALSFHQFFEGFALGGCISQAQFRNKSATI 300

Query: 331 MAVFFAITTPLGIAVGAAVASTYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLN 390
           MA FFA+TTPLGI +G AVAS++N +S GALV EGILDSLSAGILVYMALVDLIAADFL+
Sbjct: 301 MACFFALTTPLGIGIGTAVASSFNSHSPGALVTEGILDSLSAGILVYMALVDLIAADFLS 360

Query: 391 KRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 418
           KRMSCN RLQ+VSY  LFLGAGLMS+LA+WA
Sbjct: 361 KRMSCNLRLQVVSYVMLFLGAGLMSALAIWA 374

BLAST of Bhi04G000456 vs. ExPASy Swiss-Prot
Match: Q5Z653 (Zinc transporter 10 OS=Oryza sativa subsp. japonica OX=39947 GN=ZIP10 PE=3 SV=2)

HSP 1 Score: 416.0 bits (1068), Expect = 5.0e-115
Identity = 241/405 (59.51%), Postives = 283/405 (69.88%), Query Frame = 0

Query: 27  FIRTVSESMANSSC------GVSEVELCRNDTAALRYKLIAIASILTSGVIGIALPLLGR 86
           FIR ++ S++ +SC      G  + E CR++ AALR K++A+A+IL +G  G+A+PL+GR
Sbjct: 12  FIRQIAASVSAASCDAVVGGGGDKDEECRDEAAALRLKMVAVAAILIAGAAGVAIPLVGR 71

Query: 87  QCR-------FLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSDALSDPCLPEFPWSKFP 146
           + R          + G LFV AKAFAAGVILAT FVHML D   ALS+PCLP  PW +FP
Sbjct: 72  RRRGGGGGGGGGASSGGLFVLAKAFAAGVILATGFVHMLHDAEHALSNPCLPHSPWRRFP 131

Query: 147 FSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVGSVEDSGLE-SGIVPAGDLN 206
           F GF AM+A+L TL+VDFVGT +YERK    R +E A        + LE  G +P GD  
Sbjct: 132 FPGFVAMLAALATLVVDFVGTHFYERKH---RQEEAAAAAEEAAAALLEDGGALPVGDGE 191

Query: 207 VKLFGEEDGGAMHIVGMHAHAAQHRHSHPQGKEACDGHVTVHDHGHGHAHGHGHSHGLDG 266
            +        AMHIVG+HAHAA HRHSH     AC G      H HGH HGH        
Sbjct: 192 GRDGRGGKRDAMHIVGIHAHAAAHRHSHAHVHGACHGGAVNDAHAHGHGHGH-------- 251

Query: 267 DDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFALGG 326
            ++    RHVVVSQILELGIVSHSVIIGLSLGVS SPCTI+PL+AALSFHQFFEGFALGG
Sbjct: 252 -EEGPSARHVVVSQILELGIVSHSVIIGLSLGVSQSPCTIKPLVAALSFHQFFEGFALGG 311

Query: 327 CISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAEGILDSLSAGILV 386
           CIS+AQ    S  LMA FFAITTP GI VGAAVAS YNPNS  ALV EGILDS+SAGIL+
Sbjct: 312 CISEAQLKNFSAFLMAFFFAITTPAGITVGAAVASFYNPNSPRALVVEGILDSMSAGILI 371

Query: 387 YMALVDLIAADFLNKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 418
           YMALVDLIAADFL+++MSCN RLQ+ SY  LFLGA  M++LALWA
Sbjct: 372 YMALVDLIAADFLSRKMSCNPRLQVGSYIALFLGAMAMAALALWA 404

BLAST of Bhi04G000456 vs. ExPASy Swiss-Prot
Match: O82643 (Zinc transporter 9 OS=Arabidopsis thaliana OX=3702 GN=ZIP9 PE=2 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 4.7e-113
Identity = 233/362 (64.36%), Postives = 273/362 (75.41%), Query Frame = 0

Query: 62  IASILTSGVIGIALPLLGRQCRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSDALSD 121
           +ASIL SG  G+++PL+G     L  +G L   AKAFAAGVILAT FVHML  GS ALSD
Sbjct: 1   MASILISGAAGVSIPLVG---TLLPLNGGLMRGAKAFAAGVILATGFVHMLSGGSKALSD 60

Query: 122 PCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG--MIRSKEE-ANRVGSVED 181
           PCLPEFPW  FPF  FFAM+A+LLTLL DF+ T YYERKQ   M +S E    +V  + D
Sbjct: 61  PCLPEFPWKMFPFPEFFAMVAALLTLLADFMITGYYERKQEKMMNQSVESLGTQVSVMSD 120

Query: 182 SGLESGIVPAGDLNVKLFGEEDGGAMHIVGMHAHAAQHRHS---HPQGKEACDGHVTVHD 241
            GLESG          L  +EDGGA+HIVGM AHA  HRHS     +G EA      V  
Sbjct: 121 PGLESGF---------LRDQEDGGALHIVGMRAHAEHHRHSLSMGAEGFEALSKRSGVSG 180

Query: 242 HGHGHAHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPL 301
           HGHGH+HGHG   GLD     SGVRHVVVSQILE+GIVSHS+IIG+SLGVSHSPCTIRPL
Sbjct: 181 HGHGHSHGHG-DVGLD-----SGVRHVVVSQILEMGIVSHSIIIGISLGVSHSPCTIRPL 240

Query: 302 IAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTG 361
           + ALSFHQFFEGFALGGC+++A+     +++MA FFAITTP+G+AVG A+AS+YN  S  
Sbjct: 241 LLALSFHQFFEGFALGGCVAEARLTPRGSAMMAFFFAITTPIGVAVGTAIASSYNSYSVA 300

Query: 362 ALVAEGILDSLSAGILVYMALVDLIAADFLNKRMSCNFRLQIVSYCTLFLGAGLMSSLAL 418
           ALVAEG+LDSLSAGILVYMALVDLIAADFL+K+MS +FR+Q+VSYC LFLGAG+MS+LA+
Sbjct: 301 ALVAEGVLDSLSAGILVYMALVDLIAADFLSKKMSVDFRVQVVSYCFLFLGAGMMSALAI 344

BLAST of Bhi04G000456 vs. ExPASy Swiss-Prot
Match: Q6L8F7 (Zinc transporter 7 OS=Oryza sativa subsp. japonica OX=39947 GN=ZIP7 PE=2 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 4.1e-101
Identity = 220/393 (55.98%), Postives = 267/393 (67.94%), Query Frame = 0

Query: 31  VSESMANSSC----GVSEVELCRNDTAALRYKLIAIASILTSGVIGIALPLLGRQCRFLK 90
           ++ S+A  SC      +E   CR+D AALR K +A+A+IL +GV+G+ LPL GR+ R L+
Sbjct: 15  MAASLAAGSCAEEVAKAEGAGCRDDAAALRLKGVAMATILVAGVVGVGLPLAGRKRRALR 74

Query: 91  TDGNLFVAAKAFAAGVILATAFVHMLPDGSDALSDPCLPEFPWSKFPFSGFFAMMASLLT 150
           TD   FVAAKAFAAGVILAT FVHML D   ALS PCLP  PW  FPF GF AM A+L T
Sbjct: 75  TDSAAFVAAKAFAAGVILATGFVHMLHDAEHALSSPCLPAHPWRSFPFPGFVAMSAALAT 134

Query: 151 LLVDFVGTQYYERKQGMIRSKEEANRVGSVEDSGLESGIVPAGDLNVKLFGEEDG-GAMH 210
           L++DF+ T++YE K      + E  RV +   + L +      D+ V    E+D      
Sbjct: 135 LVLDFLATRFYEGKH-----RAETERVKAAAAAALAASSASDDDITVVTVTEDDNDNKAP 194

Query: 211 IVGMHAHAAQHRHSHPQGKEACDGHVTVHDHGHGHAHGHGHSHGLDGDDD-ESGVRHVVV 270
           ++  H+H+    HSHP              HGHGH H      G  G+ +  + VR VVV
Sbjct: 195 LLQPHSHS----HSHP--------------HGHGHGHELAQPEGSGGEGEVPAQVRSVVV 254

Query: 271 SQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLST 330
           SQILE+GIVSHSVIIGLSLGVS SPCTIRPL+AALSFHQFFEGFALGGCI+QAQF TLS 
Sbjct: 255 SQILEMGIVSHSVIIGLSLGVSRSPCTIRPLVAALSFHQFFEGFALGGCIAQAQFKTLSA 314

Query: 331 SLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADF 390
           ++MA FFAITTP GIA GA VAS YN NS  ALV EGILDS+SAGIL+YM+LVDLIAADF
Sbjct: 315 AIMACFFAITTPAGIAAGAGVASFYNANSPRALVVEGILDSVSAGILIYMSLVDLIAADF 374

Query: 391 LNKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 418
           L  +M+ + R Q+++Y  LFLGA  MSSLA+WA
Sbjct: 375 LGGKMTGSTRQQVMAYIALFLGALSMSSLAIWA 384

BLAST of Bhi04G000456 vs. NCBI nr
Match: XP_038886495.1 (zinc transporter 4, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 805.1 bits (2078), Expect = 2.9e-229
Identity = 417/417 (100.00%), Postives = 417/417 (100.00%), Query Frame = 0

Query: 1   MFFFEDLLQMICIRGFQDKTRLLSESFIRTVSESMANSSCGVSEVELCRNDTAALRYKLI 60
           MFFFEDLLQMICIRGFQDKTRLLSESFIRTVSESMANSSCGVSEVELCRNDTAALRYKLI
Sbjct: 1   MFFFEDLLQMICIRGFQDKTRLLSESFIRTVSESMANSSCGVSEVELCRNDTAALRYKLI 60

Query: 61  AIASILTSGVIGIALPLLGRQCRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSDALS 120
           AIASILTSGVIGIALPLLGRQCRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSDALS
Sbjct: 61  AIASILTSGVIGIALPLLGRQCRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSDALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVGSVEDSG 180
           DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVGSVEDSG
Sbjct: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVGSVEDSG 180

Query: 181 LESGIVPAGDLNVKLFGEEDGGAMHIVGMHAHAAQHRHSHPQGKEACDGHVTVHDHGHGH 240
           LESGIVPAGDLNVKLFGEEDGGAMHIVGMHAHAAQHRHSHPQGKEACDGHVTVHDHGHGH
Sbjct: 181 LESGIVPAGDLNVKLFGEEDGGAMHIVGMHAHAAQHRHSHPQGKEACDGHVTVHDHGHGH 240

Query: 241 AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300
           AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS
Sbjct: 241 AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300

Query: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE 360
           FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE
Sbjct: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE 360

Query: 361 GILDSLSAGILVYMALVDLIAADFLNKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 418
           GILDSLSAGILVYMALVDLIAADFLNKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA
Sbjct: 361 GILDSLSAGILVYMALVDLIAADFLNKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 417

BLAST of Bhi04G000456 vs. NCBI nr
Match: XP_004142927.1 (zinc transporter 4, chloroplastic isoform X1 [Cucumis sativus] >KGN62388.1 hypothetical protein Csa_018631 [Cucumis sativus])

HSP 1 Score: 765.4 bits (1975), Expect = 2.6e-217
Identity = 393/417 (94.24%), Postives = 409/417 (98.08%), Query Frame = 0

Query: 1   MFFFEDLLQMICIRGFQDKTRLLSESFIRTVSESMANSSCGVSEVELCRNDTAALRYKLI 60
           MFFFEDLLQM+CIRGFQDKTRLLSESFIRTVSESMANS+CGVSE+ELCRND+AAL +KLI
Sbjct: 1   MFFFEDLLQMLCIRGFQDKTRLLSESFIRTVSESMANSACGVSELELCRNDSAALHFKLI 60

Query: 61  AIASILTSGVIGIALPLLGRQCRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSDALS 120
           AIASILT+GVIGIALPL G+Q RFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGS ALS
Sbjct: 61  AIASILTAGVIGIALPLFGKQRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVGSVEDSG 180
           DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG+IRSKEEANRVGS+ D+G
Sbjct: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGIIRSKEEANRVGSLMDTG 180

Query: 181 LESGIVPAGDLNVKLFGEEDGGAMHIVGMHAHAAQHRHSHPQGKEACDGHVTVHDHGHGH 240
           LESGIVPAGDLNVK+FGEE+GG MHIVGMHAHAA HRH+HPQGKEACDGH+ VHDHGHGH
Sbjct: 181 LESGIVPAGDLNVKVFGEEEGGGMHIVGMHAHAAHHRHNHPQGKEACDGHMGVHDHGHGH 240

Query: 241 AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300
           AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS
Sbjct: 241 AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300

Query: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE 360
           FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE
Sbjct: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE 360

Query: 361 GILDSLSAGILVYMALVDLIAADFLNKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 418
           GILDSLSAGILVYMALVDLIAADFL+KRMSCNFRLQ+VSYCTLFLGAGLMSSLALWA
Sbjct: 361 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQLVSYCTLFLGAGLMSSLALWA 417

BLAST of Bhi04G000456 vs. NCBI nr
Match: XP_008444462.1 (PREDICTED: zinc transporter 4, chloroplastic [Cucumis melo])

HSP 1 Score: 762.7 bits (1968), Expect = 1.7e-216
Identity = 392/417 (94.00%), Postives = 407/417 (97.60%), Query Frame = 0

Query: 1   MFFFEDLLQMICIRGFQDKTRLLSESFIRTVSESMANSSCGVSEVELCRNDTAALRYKLI 60
           MFFFEDLLQ++CIRGFQDKTRLLSESFIRTVSESM NS+CGVSEVELCRND+AAL +KLI
Sbjct: 1   MFFFEDLLQVLCIRGFQDKTRLLSESFIRTVSESMTNSACGVSEVELCRNDSAALHFKLI 60

Query: 61  AIASILTSGVIGIALPLLGRQCRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSDALS 120
           AIASILT+GVIGIA+PL G+Q RFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGS ALS
Sbjct: 61  AIASILTAGVIGIAIPLFGKQRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVGSVEDSG 180
           DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVGS+ D+G
Sbjct: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVGSLVDTG 180

Query: 181 LESGIVPAGDLNVKLFGEEDGGAMHIVGMHAHAAQHRHSHPQGKEACDGHVTVHDHGHGH 240
           LESGIVPAG+LNVK+FGEEDGG MHIVGMHAHAA HRH+HPQGKEACDGHV VHDHGHGH
Sbjct: 181 LESGIVPAGELNVKVFGEEDGGGMHIVGMHAHAAHHRHNHPQGKEACDGHVAVHDHGHGH 240

Query: 241 AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300
           AHGHGHSHGLD DDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS
Sbjct: 241 AHGHGHSHGLDVDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300

Query: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE 360
           FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE
Sbjct: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE 360

Query: 361 GILDSLSAGILVYMALVDLIAADFLNKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 418
           GILDSLSAGILVYMALVDLIAADFL+KRMSCNFRLQ+VSYCTLFLGAGLMSSLALWA
Sbjct: 361 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQLVSYCTLFLGAGLMSSLALWA 417

BLAST of Bhi04G000456 vs. NCBI nr
Match: XP_031737133.1 (zinc transporter 4, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 748.4 bits (1931), Expect = 3.3e-212
Identity = 384/408 (94.12%), Postives = 400/408 (98.04%), Query Frame = 0

Query: 10  MICIRGFQDKTRLLSESFIRTVSESMANSSCGVSEVELCRNDTAALRYKLIAIASILTSG 69
           M+CIRGFQDKTRLLSESFIRTVSESMANS+CGVSE+ELCRND+AAL +KLIAIASILT+G
Sbjct: 1   MLCIRGFQDKTRLLSESFIRTVSESMANSACGVSELELCRNDSAALHFKLIAIASILTAG 60

Query: 70  VIGIALPLLGRQCRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSDALSDPCLPEFPW 129
           VIGIALPL G+Q RFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGS ALSDPCLPEFPW
Sbjct: 61  VIGIALPLFGKQRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALSDPCLPEFPW 120

Query: 130 SKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVGSVEDSGLESGIVPAG 189
           SKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG+IRSKEEANRVGS+ D+GLESGIVPAG
Sbjct: 121 SKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGIIRSKEEANRVGSLMDTGLESGIVPAG 180

Query: 190 DLNVKLFGEEDGGAMHIVGMHAHAAQHRHSHPQGKEACDGHVTVHDHGHGHAHGHGHSHG 249
           DLNVK+FGEE+GG MHIVGMHAHAA HRH+HPQGKEACDGH+ VHDHGHGHAHGHGHSHG
Sbjct: 181 DLNVKVFGEEEGGGMHIVGMHAHAAHHRHNHPQGKEACDGHMGVHDHGHGHAHGHGHSHG 240

Query: 250 LDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFA 309
           LDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFA
Sbjct: 241 LDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFA 300

Query: 310 LGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAEGILDSLSAG 369
           LGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAEGILDSLSAG
Sbjct: 301 LGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAEGILDSLSAG 360

Query: 370 ILVYMALVDLIAADFLNKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 418
           ILVYMALVDLIAADFL+KRMSCNFRLQ+VSYCTLFLGAGLMSSLALWA
Sbjct: 361 ILVYMALVDLIAADFLSKRMSCNFRLQLVSYCTLFLGAGLMSSLALWA 408

BLAST of Bhi04G000456 vs. NCBI nr
Match: XP_022962014.1 (zinc transporter 4, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 735.3 bits (1897), Expect = 2.8e-208
Identity = 378/417 (90.65%), Postives = 397/417 (95.20%), Query Frame = 0

Query: 1   MFFFEDLLQMICIRGFQDKTRLLSESFIRTVSESMANSSCGVSEVELCRNDTAALRYKLI 60
           MFF E+L QM+C++GFQDK R+LSES IRTVSESM NS CGVSE+ELCR+D+AA  +KLI
Sbjct: 1   MFFLENLWQMLCVQGFQDKARVLSESLIRTVSESMGNSDCGVSELELCRDDSAAFHFKLI 60

Query: 61  AIASILTSGVIGIALPLLGRQCRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSDALS 120
           AIASILTSGVIGIA+PL G Q RFLKTDGNLFV AKAFAAGVILATAFVHMLPDGS ALS
Sbjct: 61  AIASILTSGVIGIAIPLFGSQRRFLKTDGNLFVTAKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVGSVEDSG 180
           DPCLP+FPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG+IRSKEE NRVGSVEDSG
Sbjct: 121 DPCLPKFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGVIRSKEEENRVGSVEDSG 180

Query: 181 LESGIVPAGDLNVKLFGEEDGGAMHIVGMHAHAAQHRHSHPQGKEACDGHVTVHDHGHGH 240
           LESGI+PAGDLNVK+FGEEDGGAMHI+GMHAHAA HRHSHPQGKEACDGHV VHDHGHG 
Sbjct: 181 LESGILPAGDLNVKVFGEEDGGAMHIIGMHAHAAHHRHSHPQGKEACDGHVRVHDHGHG- 240

Query: 241 AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300
             GHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS
Sbjct: 241 --GHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300

Query: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE 360
           FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTP+GIA+G AVASTYNPNSTGAL+AE
Sbjct: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPIGIAIGTAVASTYNPNSTGALIAE 360

Query: 361 GILDSLSAGILVYMALVDLIAADFLNKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 418
           GILDSLSAGILVYMALVDLIAADFL+KRMSCNFRLQIVSYCTLFLGAGLMSSLALWA
Sbjct: 361 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 414

BLAST of Bhi04G000456 vs. ExPASy TrEMBL
Match: A0A0A0LKS6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G351690 PE=3 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 1.2e-217
Identity = 393/417 (94.24%), Postives = 409/417 (98.08%), Query Frame = 0

Query: 1   MFFFEDLLQMICIRGFQDKTRLLSESFIRTVSESMANSSCGVSEVELCRNDTAALRYKLI 60
           MFFFEDLLQM+CIRGFQDKTRLLSESFIRTVSESMANS+CGVSE+ELCRND+AAL +KLI
Sbjct: 1   MFFFEDLLQMLCIRGFQDKTRLLSESFIRTVSESMANSACGVSELELCRNDSAALHFKLI 60

Query: 61  AIASILTSGVIGIALPLLGRQCRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSDALS 120
           AIASILT+GVIGIALPL G+Q RFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGS ALS
Sbjct: 61  AIASILTAGVIGIALPLFGKQRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVGSVEDSG 180
           DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG+IRSKEEANRVGS+ D+G
Sbjct: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGIIRSKEEANRVGSLMDTG 180

Query: 181 LESGIVPAGDLNVKLFGEEDGGAMHIVGMHAHAAQHRHSHPQGKEACDGHVTVHDHGHGH 240
           LESGIVPAGDLNVK+FGEE+GG MHIVGMHAHAA HRH+HPQGKEACDGH+ VHDHGHGH
Sbjct: 181 LESGIVPAGDLNVKVFGEEEGGGMHIVGMHAHAAHHRHNHPQGKEACDGHMGVHDHGHGH 240

Query: 241 AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300
           AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS
Sbjct: 241 AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300

Query: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE 360
           FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE
Sbjct: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE 360

Query: 361 GILDSLSAGILVYMALVDLIAADFLNKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 418
           GILDSLSAGILVYMALVDLIAADFL+KRMSCNFRLQ+VSYCTLFLGAGLMSSLALWA
Sbjct: 361 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQLVSYCTLFLGAGLMSSLALWA 417

BLAST of Bhi04G000456 vs. ExPASy TrEMBL
Match: A0A1S3BAD0 (zinc transporter 4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487782 PE=3 SV=1)

HSP 1 Score: 762.7 bits (1968), Expect = 8.1e-217
Identity = 392/417 (94.00%), Postives = 407/417 (97.60%), Query Frame = 0

Query: 1   MFFFEDLLQMICIRGFQDKTRLLSESFIRTVSESMANSSCGVSEVELCRNDTAALRYKLI 60
           MFFFEDLLQ++CIRGFQDKTRLLSESFIRTVSESM NS+CGVSEVELCRND+AAL +KLI
Sbjct: 1   MFFFEDLLQVLCIRGFQDKTRLLSESFIRTVSESMTNSACGVSEVELCRNDSAALHFKLI 60

Query: 61  AIASILTSGVIGIALPLLGRQCRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSDALS 120
           AIASILT+GVIGIA+PL G+Q RFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGS ALS
Sbjct: 61  AIASILTAGVIGIAIPLFGKQRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVGSVEDSG 180
           DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVGS+ D+G
Sbjct: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVGSLVDTG 180

Query: 181 LESGIVPAGDLNVKLFGEEDGGAMHIVGMHAHAAQHRHSHPQGKEACDGHVTVHDHGHGH 240
           LESGIVPAG+LNVK+FGEEDGG MHIVGMHAHAA HRH+HPQGKEACDGHV VHDHGHGH
Sbjct: 181 LESGIVPAGELNVKVFGEEDGGGMHIVGMHAHAAHHRHNHPQGKEACDGHVAVHDHGHGH 240

Query: 241 AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300
           AHGHGHSHGLD DDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS
Sbjct: 241 AHGHGHSHGLDVDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300

Query: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE 360
           FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE
Sbjct: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE 360

Query: 361 GILDSLSAGILVYMALVDLIAADFLNKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 418
           GILDSLSAGILVYMALVDLIAADFL+KRMSCNFRLQ+VSYCTLFLGAGLMSSLALWA
Sbjct: 361 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQLVSYCTLFLGAGLMSSLALWA 417

BLAST of Bhi04G000456 vs. ExPASy TrEMBL
Match: A0A6J1HFN1 (zinc transporter 4, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111462610 PE=3 SV=1)

HSP 1 Score: 735.3 bits (1897), Expect = 1.4e-208
Identity = 378/417 (90.65%), Postives = 397/417 (95.20%), Query Frame = 0

Query: 1   MFFFEDLLQMICIRGFQDKTRLLSESFIRTVSESMANSSCGVSEVELCRNDTAALRYKLI 60
           MFF E+L QM+C++GFQDK R+LSES IRTVSESM NS CGVSE+ELCR+D+AA  +KLI
Sbjct: 1   MFFLENLWQMLCVQGFQDKARVLSESLIRTVSESMGNSDCGVSELELCRDDSAAFHFKLI 60

Query: 61  AIASILTSGVIGIALPLLGRQCRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSDALS 120
           AIASILTSGVIGIA+PL G Q RFLKTDGNLFV AKAFAAGVILATAFVHMLPDGS ALS
Sbjct: 61  AIASILTSGVIGIAIPLFGSQRRFLKTDGNLFVTAKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVGSVEDSG 180
           DPCLP+FPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG+IRSKEE NRVGSVEDSG
Sbjct: 121 DPCLPKFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGVIRSKEEENRVGSVEDSG 180

Query: 181 LESGIVPAGDLNVKLFGEEDGGAMHIVGMHAHAAQHRHSHPQGKEACDGHVTVHDHGHGH 240
           LESGI+PAGDLNVK+FGEEDGGAMHI+GMHAHAA HRHSHPQGKEACDGHV VHDHGHG 
Sbjct: 181 LESGILPAGDLNVKVFGEEDGGAMHIIGMHAHAAHHRHSHPQGKEACDGHVRVHDHGHG- 240

Query: 241 AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300
             GHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS
Sbjct: 241 --GHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300

Query: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE 360
           FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTP+GIA+G AVASTYNPNSTGAL+AE
Sbjct: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPIGIAIGTAVASTYNPNSTGALIAE 360

Query: 361 GILDSLSAGILVYMALVDLIAADFLNKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 418
           GILDSLSAGILVYMALVDLIAADFL+KRMSCNFRLQIVSYCTLFLGAGLMSSLALWA
Sbjct: 361 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 414

BLAST of Bhi04G000456 vs. ExPASy TrEMBL
Match: A0A6J1K6U3 (zinc transporter 4, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111491704 PE=3 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 8.9e-208
Identity = 377/417 (90.41%), Postives = 397/417 (95.20%), Query Frame = 0

Query: 1   MFFFEDLLQMICIRGFQDKTRLLSESFIRTVSESMANSSCGVSEVELCRNDTAALRYKLI 60
           MFF EDL Q++C++GFQDK R+LSES +RTVSESM NS CGVSE+ELCR+D+AA  +KLI
Sbjct: 1   MFFLEDLWQILCVQGFQDKARVLSESLLRTVSESMGNSDCGVSELELCRDDSAAFHFKLI 60

Query: 61  AIASILTSGVIGIALPLLGRQCRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSDALS 120
           AIASILTSGVIGIA+PL G Q RFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGS+ALS
Sbjct: 61  AIASILTSGVIGIAIPLFGSQRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSNALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVGSVEDSG 180
           DPCLP+FPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG+IRSKEE NRVGSV DSG
Sbjct: 121 DPCLPKFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGVIRSKEEENRVGSVVDSG 180

Query: 181 LESGIVPAGDLNVKLFGEEDGGAMHIVGMHAHAAQHRHSHPQGKEACDGHVTVHDHGHGH 240
           LESGI+PAGDLNVKLFGEEDGGAMHIVGMHAHA  HR SHPQGKEACDGHV VHDHGH H
Sbjct: 181 LESGILPAGDLNVKLFGEEDGGAMHIVGMHAHAGHHRPSHPQGKEACDGHVRVHDHGHAH 240

Query: 241 AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300
             GHGHSHGLDGDDDESGVRHVV+SQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS
Sbjct: 241 G-GHGHSHGLDGDDDESGVRHVVISQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300

Query: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE 360
           FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTP+GIA+GAAVASTYNPNSTGAL+AE
Sbjct: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPIGIAIGAAVASTYNPNSTGALIAE 360

Query: 361 GILDSLSAGILVYMALVDLIAADFLNKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 418
           GILDSLSAGILVYMALVDLIAADFL+KRMSCNFRLQIVSYCTLFLGAGLMSSLALWA
Sbjct: 361 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 416

BLAST of Bhi04G000456 vs. ExPASy TrEMBL
Match: A0A6J1GGQ1 (zinc transporter 4, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111454070 PE=3 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 4.8e-201
Identity = 368/417 (88.25%), Postives = 387/417 (92.81%), Query Frame = 0

Query: 1   MFFFEDLLQMICIRGFQDKTRLLSESFIRTVSESMANSSCGVSEVELCRNDTAALRYKLI 60
           M FFEDL QM+   GFQD+TRLLSES +RTVSESM NSSC VSE+ELCR+D+AAL +KLI
Sbjct: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60

Query: 61  AIASILTSGVIGIALPLLGRQCRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSDALS 120
           AIASILTSGV+GIA+PL GR  RFLKTDGNLFVA KAFAAGVILATAFVHMLPDGS ALS
Sbjct: 61  AIASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVGSVEDSG 180
           DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG IR KEE NRVGS  DSG
Sbjct: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDSG 180

Query: 181 LESGIVPAGDLNVKLFGEEDGGAMHIVGMHAHAAQHRHSHPQGKEACDGHVTVHDHGHGH 240
           LE+GI+PA D NVK+FGEEDGG MHIVGMHAHAAQHRHSHPQGK+ACDGHVT+HD     
Sbjct: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKDACDGHVTLHD----- 240

Query: 241 AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300
            HGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS
Sbjct: 241 -HGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300

Query: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE 360
           FHQFFEGFALGGCISQAQFNTLSTSLMAVFFA TTP+GIA+GAA++STYNPNSTGALVAE
Sbjct: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAE 360

Query: 361 GILDSLSAGILVYMALVDLIAADFLNKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 418
           GILDSLSAGILVYMALVDLIAADFL+KRMSCNFRLQIVSYCTLFLGAGLMS LALWA
Sbjct: 361 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 411

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G60960.17.4e-14664.25iron regulated transporter 3 [more]
AT1G10970.13.5e-14064.47zinc transporter 4 precursor [more]
AT4G33020.13.3e-11464.36ZIP metal ion transporter family [more]
AT1G05300.18.9e-7542.46zinc transporter 5 precursor [more]
AT3G12750.18.6e-7042.34zinc transporter 1 precursor [more]
Match NameE-valueIdentityDescription
Q8LE591.0e-14464.25Fe(2+) transport protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IRT... [more]
O040891.5e-13567.26Zinc transporter 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ZIP4 PE=2 S... [more]
Q5Z6535.0e-11559.51Zinc transporter 10 OS=Oryza sativa subsp. japonica OX=39947 GN=ZIP10 PE=3 SV=2[more]
O826434.7e-11364.36Zinc transporter 9 OS=Arabidopsis thaliana OX=3702 GN=ZIP9 PE=2 SV=1[more]
Q6L8F74.1e-10155.98Zinc transporter 7 OS=Oryza sativa subsp. japonica OX=39947 GN=ZIP7 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038886495.12.9e-229100.00zinc transporter 4, chloroplastic-like [Benincasa hispida][more]
XP_004142927.12.6e-21794.24zinc transporter 4, chloroplastic isoform X1 [Cucumis sativus] >KGN62388.1 hypot... [more]
XP_008444462.11.7e-21694.00PREDICTED: zinc transporter 4, chloroplastic [Cucumis melo][more]
XP_031737133.13.3e-21294.12zinc transporter 4, chloroplastic isoform X2 [Cucumis sativus][more]
XP_022962014.12.8e-20890.65zinc transporter 4, chloroplastic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0LKS61.2e-21794.24Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G351690 PE=3 SV=1[more]
A0A1S3BAD08.1e-21794.00zinc transporter 4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487782 PE=3 S... [more]
A0A6J1HFN11.4e-20890.65zinc transporter 4, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A6J1K6U38.9e-20890.41zinc transporter 4, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114917... [more]
A0A6J1GGQ14.8e-20188.25zinc transporter 4, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003689Zinc/iron permeasePFAMPF02535Zipcoord: 57..414
e-value: 1.5E-76
score: 257.8
IPR004698Zinc/iron permease, fungal/plantTIGRFAMTIGR00820TIGR00820coord: 40..417
e-value: 1.3E-133
score: 443.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 235..255
NoneNo IPR availablePANTHERPTHR11040ZINC/IRON TRANSPORTERcoord: 35..417
NoneNo IPR availablePANTHERPTHR11040:SF157ZIP ZINC/IRON TRANSPORT FAMILY PROTEINcoord: 35..417

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi04M000456Bhi04M000456mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071577 zinc ion transmembrane transport
biological_process GO:0030001 metal ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0005385 zinc ion transmembrane transporter activity
molecular_function GO:0046873 metal ion transmembrane transporter activity