Bhi04G000193 (gene) Wax gourd (B227) v1

Overview
NameBhi04G000193
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionWAT1-related protein
Locationchr4: 5167130 .. 5172083 (-)
RNA-Seq ExpressionBhi04G000193
SyntenyBhi04G000193
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGACACAGGCTCCGTCTCCAACAAGAGAATGTCGTGTTCTATCCCCGAGAGGTTCCAGCTCCATGCCGCCATGTTGGCCTTGCAATTCGGCTATGCCGGTTTCCATGTCGTCTCTAGAGCTGCCCTCAACATGGGCATTAGTAAACTCGTCTTCCCTGTCTACCGAAATGTCATTGCTTTGCTCCTCCTCCTTCCCTTTGCATATTTCATCGAAAAGTAAGCTTACGAATATGTATGACAATTTTACACCGTGATTACAAAAATAAAATATCTCCCGACTTGTATTTTCTTTCAAAAATATTCACGTTCTATCAAAAAAATTGAAATAGTACATTTGACATTTTTATTAAAAACTACACTTAAAAGTAAAACTTTATTAGAATTTTAGATGAAAGTTTGTGACCTGAGATCATGTTCTCTGACAAACTAAAATGAAACTTAGGTCATCACTACACTTATTTAATTTTATGTCTCGATTTTCATTCAAAATTTTAACAGAGTATTACTAACTCATAATATTGCACGATCATGGAAGTATTCACATAATTTCAATATATATCTAACGATTATTACGATGTTCATTTGTTTTTTTGGCACAATCAGGAAGGATAGGCCTGCCCTCACTCTCAACTTTGTCCTTCAATTCTTCCTACTAGCTCTTGTTGGGTAATAAAAATATGAAAGAATTGAATTTTTGTTATGAGAGAAAAAATATATATTTTTTAATTTTCTGGATTTAATTAAACTGACTAATGTTTTTTTTTTTTTTTTTTTTTTAATGTGGGCAGAATTACAGCCAATCAAGGGTTTTACTTGCTGGGTTTGGATAATACTTCCCCAACCTTTGCTTCTGCTATTCAAAATTCTGTCCCTGCCATTACTTTCCTTATGGCTGCACTTCTCAGGTAATAATAATATTAATTACACAAATTTTTAATTTAAATTACTCCCTCTAACACTCCATTTAACAACTAAACCAAAAACTTCTTTAATTTCTTCATTAAATTTAACCCTAAGTTTTACAATACTGCATGCCTTGCCCACATCATCTATCTAATTAGTTTCAAAGTCTAAGAATCTTTAATTTGGATGGTAATTAAGTTTAATAAATATCATACACAAAATGCACCTAATAATTAACTCAGGATTATACAATTCTTTTTCTTTTTGTTTGAGTTGTCAAATCCACCCTTTAAAATTTTAATTTATTTGTCTACTAGAAATGATTTTTTTTTAAAAAAAATAGTTAAATCACAACTAAATTTAAAAGTCAAAGTTCATGGAGTTTGATAAATTTAATTGTATTAATTATTTAATACATCGTTTTTATGTGTAGGTTTGAATATTTGTAAAAGGTCCAATAACCAAAAACCAATCATATTATTTTTTTCAAAAAAATAACATTAAAGATTTTGAACAGAAACCTCACACTTTGATACTCTACATACGTTAAATATTAACCCATCAACTCAGGAGCTCAAACTATGGATTAAAAAATTTACTATAGACTAGTGATTTCAATTTTATGACTTATTAATTTTTTTAAATTATGGATCTCTTATTGTTAAATAATACTGAAAACTAAAAAATTTTATTTATTGTCCCATATGGCCCTTTAATCATAAGTCACACTCATTGGTTTAAGTTTTTGAGTTGATAAATACAGACGGTTTTGTATTATTTAACATACTAATTCTTCTCCCTTTGTTTTGCCATCAGGATTGAGCAAGTGCGACTGAACCGAAAAGATGGGATAGCAAAGGTGATGGGTACAATATGTTGTGTGGCCGGAGCGACGGTGATCACATTATACAAAGGGCCCACCATCTACAGCCCGGCAACGACGACGGTGACGGTGCAGGGGACGGCAGAGACGGCGGGGACGATGCTGATGGGGCCAGGAATGTTCCCATCCTTAGGCGATGCAAAGGGGAAAAGCTGGACGTTAGGGTGCGTGTACTTGATCGGACACTGCTTGTCGTGGTCGGCGTGGCTAGTCCTACAAGCACCGGTGCTGAAAAAATACCCGGCTCGCCTCTCCGTCACCTCCTTCACTTGCTTTTTTGGTCTCATCCAATTTCTCATCATCGCCATCGCGTTCGAGCGCGATGCTCAGGCTTGGCTCTTCCACTCCGGCGCCGAAGCCTTTAGCATCGTTTATGCTGTAAGTCGAAGTTTTTTCCTAACTAAAAACCTAGGCTAAATTTCAAAAAGAAATCATTTTTGAAAAACTACTTTTGTTTTGTTAGTGTTTATAATTAATTAGGATGACTTTTCAAAAATCTAATGATTATCGAATTTGTAAATTTTTTTTTTAGAGTTTTATACAAAATTGAAATTTTAAGATTCTATTAGTCTATAAAATGCAAATTTATGTCTAATAAATCAAATAATTTCTAAAAAATTTCAATTTTGGTAGGAATTTTTTAGACAACAAAAGAAAAGTTTATAAACTTATTCTTAAATCATCGACCTAAAAATTTAAAATTTTTAGTGAAGGTAAATTTAATTACCTTCTAAACCAACCTACTTTTATACCATCTTAAATCACTTATTGACCTAAAAGTTTAAGTTAATCGATGAAGACTAGTTTAATTTTATATCATCTTACACTTACTAGACATTTTTAAAAGCGAAGGGACTCGATGACACCAAGTAGTAACCTCAAAGTTTAAGAACTAAACTCATAATTTAACTCTTAGTGTATCTTTTTTTTTTTTTTGTGGCTAAAAATTTTTAGTGGATATTGTGATCTAAATTTAGTTTTTAAGATTAGTATTTTTTGTTCCTCAACTTCTATAAAGGTTACAGGTTAGTTCCTACACTTTAGTTTATAATGATTTAATTTATCACGGTGTATTTTCAAATTTAAAACAATTTAGTCTATGAACTTTAGTAATAATCTAATATTCCTTGTACTTTATCATTTATAAATATTGAATCTCCATCCATGAAAGTTAATAATAAAATTTAATGAAAGTTAGAACACGTGGAGATTAATAAAATAATTAGAAATCAAACTTGTTTATAAATTATAAAAACTAATGTTTTTAGACATTAAAATTGTACAAATAATTTTTCAAGAGACTAATATATTGATACGGACTCAATTATTATGTGTAGAAAAGTTTCCCTTAAAAAAAAAAAAAAAATTAATTATTAATTTAGAGTTTTGGTTGGTTTAAATGAATGTGGGTGTAGGGGGTGGTGGCTTCGGGGATAGCGTTTGCTGTACAGATATGGTGCATTGACAGAGGTGGCCCGGTCTTCGTTGCTGTCTACCAACCGGTTCAAACCTTCGTCGTTGCTCTCATGGCTTCCTTCGCTTTGGGCGAGGAGTTCTACTTGGGAGGGTACCTCTTTTTTCATCTTTCATTTAATCTCAACCCTTCATCAAATCCATCCATTCAATACCTACTTACAAATTAATCTAAAAATTTGTTAGACAGAAAATTAAAATGTCAAAACCTTAAATAAAATTAAAAAAAAACATTTCTTTTATTATTATTATTTAAAAAGAAAAAGTGTCTAAGGAGATTAGAAACTTGGTTGATTTAATGTTAGGATGTACACATAAAAGTTTTAACACCAAAAGTTGAATTAGTTGAACTATGATTAAGTTAATTAATCATTAAAATATTTTAAAATAAATTATAAATTTAATTCTTAAACTTTTGAATTTGAATCTTGAACTTTTACGAGTGTAATATATTTCTTGAATTTTCAACTTTTTGTTTAATAGGTCCCTGTAATTTAAAAATATCTAATATTGTCCTTAAACTTTCACTTTTTTATTTAATAAGTCTTTGACATGTTTAAAAAATTATTAAATAATTGGTCTATTAAATTAAATTAAGTCTTATGTTTAATATGTTAATAAACTTTCAATTTTATATTAGTAGATTTGTATAATATATATATATAAAAGTCTGATATTTAAACATAAAATTGAATGTTAGAGACCTAAATTTATGAAACAAATATACATAATCCTTAAATTCATAAATTTGGAAATTAAAAATTGTAATTTAAGCAAGGTGTTAAGGACCTCTTTTGTAGGCAATCTAAAAGCAGAAATTTGAAAATAAGAGATTCATTGTCATAAAATAAATTCTAATTTTAAAAAAATTTCAAAATATGATTGAAAATATATTTAGTTACTCATTAAATAATAAGTTGAATATAAACCATTATTAATTAATTTCTGAACTTGTTTTATGTTAAAATTTTTATTATTTTGTAATATCATAAAAAATATATATAATATATTACATATTTTAAGTTTAACAAAAAATAGTTGAGTAAGTTGTTATTGTTTTTATCATCTTTTATATAATTTTGCATTTTAAAATTTTGAATTCAAAATCTGAAAACACTAAAAGCATAAAAAAATTATTTTCAGAATTTGTACGGTTTAGATCACGTAATCGAAAAACAACTTTCGAAAATAGAATCCATACGGCCAATCAAGCACATATCTTTCAACTTAATGAATCTGAAAATATAAAACGAAATTTGAATTTTCTACCCAATAAACCCTAATTTCTTAAATTTGTATTTATTATTTTAGATGAACAATAAACTAACCCCAACCACACGAGTCTTCTTTAATCATAAACATTGGTGGGACCTATTTAGAGATATTTTGGACACTCTTATCTTGGACAAGATAGTCCACTGTCTATTAATCTAATTATGAGGTTAAAAATATATATATATTGGTGAGACACATTGATTAAGCGTGGGTCTGGATGTACAGGATCATAGGAGCGGTGCTGATTATAACAGGACTATACCTTGTGCTGTGGGGAAAGAGCGAAGAAAAGAAGATCTTATTGGAAAGAGCAGTGATCCAGTCCGCCCCGGACCACGGTAGCAGCAGACCAAGTGGCCACATCAAATCGTCAATTACCCAGCCACTTCTCCATCCTACGGCTGAAAATGTTTGA

mRNA sequence

ATGGCGGACACAGGCTCCGTCTCCAACAAGAGAATGTCGTGTTCTATCCCCGAGAGGTTCCAGCTCCATGCCGCCATGTTGGCCTTGCAATTCGGCTATGCCGGTTTCCATGTCGTCTCTAGAGCTGCCCTCAACATGGGCATTAGTAAACTCGTCTTCCCTGTCTACCGAAATGTCATTGCTTTGCTCCTCCTCCTTCCCTTTGCATATTTCATCGAAAAGAAGGATAGGCCTGCCCTCACTCTCAACTTTGTCCTTCAATTCTTCCTACTAGCTCTTGTTGGAATTACAGCCAATCAAGGGTTTTACTTGCTGGGTTTGGATAATACTTCCCCAACCTTTGCTTCTGCTATTCAAAATTCTGTCCCTGCCATTACTTTCCTTATGGCTGCACTTCTCAGGATTGAGCAAGTGCGACTGAACCGAAAAGATGGGATAGCAAAGGTGATGGGTACAATATGTTGTGTGGCCGGAGCGACGGTGATCACATTATACAAAGGGCCCACCATCTACAGCCCGGCAACGACGACGGTGACGGTGCAGGGGACGGCAGAGACGGCGGGGACGATGCTGATGGGGCCAGGAATGTTCCCATCCTTAGGCGATGCAAAGGGGAAAAGCTGGACGTTAGGGTGCGTGTACTTGATCGGACACTGCTTGTCGTGGTCGGCGTGGCTAGTCCTACAAGCACCGGTGCTGAAAAAATACCCGGCTCGCCTCTCCGTCACCTCCTTCACTTGCTTTTTTGGTCTCATCCAATTTCTCATCATCGCCATCGCGTTCGAGCGCGATGCTCAGGCTTGGCTCTTCCACTCCGGCGCCGAAGCCTTTAGCATCGTTTATGCTGGGGTGGTGGCTTCGGGGATAGCGTTTGCTGTACAGATATGGTGCATTGACAGAGGTGGCCCGGTCTTCGTTGCTGTCTACCAACCGGTTCAAACCTTCGTCGTTGCTCTCATGGCTTCCTTCGCTTTGGGCGAGGAGTTCTACTTGGGAGGGATCATAGGAGCGGTGCTGATTATAACAGGACTATACCTTGTGCTGTGGGGAAAGAGCGAAGAAAAGAAGATCTTATTGGAAAGAGCAGTGATCCAGTCCGCCCCGGACCACGGTAGCAGCAGACCAAGTGGCCACATCAAATCGTCAATTACCCAGCCACTTCTCCATCCTACGGCTGAAAATGTTTGA

Coding sequence (CDS)

ATGGCGGACACAGGCTCCGTCTCCAACAAGAGAATGTCGTGTTCTATCCCCGAGAGGTTCCAGCTCCATGCCGCCATGTTGGCCTTGCAATTCGGCTATGCCGGTTTCCATGTCGTCTCTAGAGCTGCCCTCAACATGGGCATTAGTAAACTCGTCTTCCCTGTCTACCGAAATGTCATTGCTTTGCTCCTCCTCCTTCCCTTTGCATATTTCATCGAAAAGAAGGATAGGCCTGCCCTCACTCTCAACTTTGTCCTTCAATTCTTCCTACTAGCTCTTGTTGGAATTACAGCCAATCAAGGGTTTTACTTGCTGGGTTTGGATAATACTTCCCCAACCTTTGCTTCTGCTATTCAAAATTCTGTCCCTGCCATTACTTTCCTTATGGCTGCACTTCTCAGGATTGAGCAAGTGCGACTGAACCGAAAAGATGGGATAGCAAAGGTGATGGGTACAATATGTTGTGTGGCCGGAGCGACGGTGATCACATTATACAAAGGGCCCACCATCTACAGCCCGGCAACGACGACGGTGACGGTGCAGGGGACGGCAGAGACGGCGGGGACGATGCTGATGGGGCCAGGAATGTTCCCATCCTTAGGCGATGCAAAGGGGAAAAGCTGGACGTTAGGGTGCGTGTACTTGATCGGACACTGCTTGTCGTGGTCGGCGTGGCTAGTCCTACAAGCACCGGTGCTGAAAAAATACCCGGCTCGCCTCTCCGTCACCTCCTTCACTTGCTTTTTTGGTCTCATCCAATTTCTCATCATCGCCATCGCGTTCGAGCGCGATGCTCAGGCTTGGCTCTTCCACTCCGGCGCCGAAGCCTTTAGCATCGTTTATGCTGGGGTGGTGGCTTCGGGGATAGCGTTTGCTGTACAGATATGGTGCATTGACAGAGGTGGCCCGGTCTTCGTTGCTGTCTACCAACCGGTTCAAACCTTCGTCGTTGCTCTCATGGCTTCCTTCGCTTTGGGCGAGGAGTTCTACTTGGGAGGGATCATAGGAGCGGTGCTGATTATAACAGGACTATACCTTGTGCTGTGGGGAAAGAGCGAAGAAAAGAAGATCTTATTGGAAAGAGCAGTGATCCAGTCCGCCCCGGACCACGGTAGCAGCAGACCAAGTGGCCACATCAAATCGTCAATTACCCAGCCACTTCTCCATCCTACGGCTGAAAATGTTTGA

Protein sequence

MADTGSVSNKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVIALLLLLPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQNSVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTVQGTAETAGTMLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARLSVTSFTCFFGLIQFLIIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILLERAVIQSAPDHGSSRPSGHIKSSITQPLLHPTAENV
Homology
BLAST of Bhi04G000193 vs. TAIR 10
Match: AT1G75500.1 (Walls Are Thin 1 )

HSP 1 Score: 558.9 bits (1439), Expect = 3.2e-159
Identity = 292/399 (73.18%), Postives = 336/399 (84.21%), Query Frame = 0

Query: 1   MADTGSVSNKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60
           MAD  +  N+R    +PE+ QLH AML LQFGYAGFHVVSRAALNMGISKLVFPVYRN+I
Sbjct: 1   MAD--NTDNRRSLWGVPEKLQLHIAMLTLQFGYAGFHVVSRAALNMGISKLVFPVYRNII 60

Query: 61  ALLLLLPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120
           ALLLLLPFAYF+EKK+RPA+TLNF++QFF LAL+GITANQGFYLLGLDNTSPTFAS++QN
Sbjct: 61  ALLLLLPFAYFLEKKERPAITLNFLIQFFFLALIGITANQGFYLLGLDNTSPTFASSMQN 120

Query: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTV 180
           SVPAITFLMAALLRIE+VR+NR+DGI+K++GT  CVAGA+VITLYKGPTIY+PA+    +
Sbjct: 121 SVPAITFLMAALLRIEKVRINRRDGISKILGTALCVAGASVITLYKGPTIYTPAS---HL 180

Query: 181 QGTAETAGTMLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL 240
                T  + ++ P     LG+A  K+WTLGC+YLIGHCLSWS WLV QAPVLK YPARL
Sbjct: 181 HAHLLTTNSAVLAP-----LGNAAPKNWTLGCIYLIGHCLSWSGWLVFQAPVLKSYPARL 240

Query: 241 SVTSFTCFFGLIQFLIIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR 300
           SVTS+TCFFG+IQFLIIA   ERD+QAW+FHSG E F+I+YAG+VASGIAFAVQIWCIDR
Sbjct: 241 SVTSYTCFFGIIQFLIIAAFCERDSQAWVFHSGWELFTILYAGIVASGIAFAVQIWCIDR 300

Query: 301 GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKI-LL 360
           GGPVFVAVYQPVQT VVA+MAS ALGEEFYLGGIIGAVLII GLY VL+GKSEE+K   L
Sbjct: 301 GGPVFVAVYQPVQTLVVAIMASIALGEEFYLGGIIGAVLIIAGLYFVLYGKSEERKFAAL 360

Query: 361 ERAVIQSAPDHGSSR---PSGHIKSSITQPLLHPTAENV 396
           E+A IQS+ +HG  R       IKSSIT PLLH + +NV
Sbjct: 361 EKAAIQSSAEHGIERAPVSRNSIKSSITTPLLHQSTDNV 389

BLAST of Bhi04G000193 vs. TAIR 10
Match: AT1G75500.2 (Walls Are Thin 1 )

HSP 1 Score: 558.9 bits (1439), Expect = 3.2e-159
Identity = 292/399 (73.18%), Postives = 336/399 (84.21%), Query Frame = 0

Query: 1   MADTGSVSNKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60
           MAD  +  N+R    +PE+ QLH AML LQFGYAGFHVVSRAALNMGISKLVFPVYRN+I
Sbjct: 1   MAD--NTDNRRSLWGVPEKLQLHIAMLTLQFGYAGFHVVSRAALNMGISKLVFPVYRNII 60

Query: 61  ALLLLLPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120
           ALLLLLPFAYF+EKK+RPA+TLNF++QFF LAL+GITANQGFYLLGLDNTSPTFAS++QN
Sbjct: 61  ALLLLLPFAYFLEKKERPAITLNFLIQFFFLALIGITANQGFYLLGLDNTSPTFASSMQN 120

Query: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTV 180
           SVPAITFLMAALLRIE+VR+NR+DGI+K++GT  CVAGA+VITLYKGPTIY+PA+    +
Sbjct: 121 SVPAITFLMAALLRIEKVRINRRDGISKILGTALCVAGASVITLYKGPTIYTPAS---HL 180

Query: 181 QGTAETAGTMLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL 240
                T  + ++ P     LG+A  K+WTLGC+YLIGHCLSWS WLV QAPVLK YPARL
Sbjct: 181 HAHLLTTNSAVLAP-----LGNAAPKNWTLGCIYLIGHCLSWSGWLVFQAPVLKSYPARL 240

Query: 241 SVTSFTCFFGLIQFLIIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR 300
           SVTS+TCFFG+IQFLIIA   ERD+QAW+FHSG E F+I+YAG+VASGIAFAVQIWCIDR
Sbjct: 241 SVTSYTCFFGIIQFLIIAAFCERDSQAWVFHSGWELFTILYAGIVASGIAFAVQIWCIDR 300

Query: 301 GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKI-LL 360
           GGPVFVAVYQPVQT VVA+MAS ALGEEFYLGGIIGAVLII GLY VL+GKSEE+K   L
Sbjct: 301 GGPVFVAVYQPVQTLVVAIMASIALGEEFYLGGIIGAVLIIAGLYFVLYGKSEERKFAAL 360

Query: 361 ERAVIQSAPDHGSSR---PSGHIKSSITQPLLHPTAENV 396
           E+A IQS+ +HG  R       IKSSIT PLLH + +NV
Sbjct: 361 EKAAIQSSAEHGIERAPVSRNSIKSSITTPLLHQSTDNV 389

BLAST of Bhi04G000193 vs. TAIR 10
Match: AT3G53210.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 400.6 bits (1028), Expect = 1.5e-111
Identity = 203/378 (53.70%), Postives = 275/378 (72.75%), Query Frame = 0

Query: 16  IPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVIALLLLLPFAYFIEKK 75
           IPER +LH AM+  Q GYAG HV+ R ALN+G+SKLVFP+YR ++A  +L P AYF+EKK
Sbjct: 4   IPERAKLHIAMVVFQTGYAGNHVIMRYALNLGVSKLVFPLYRTIVAFSVLAPSAYFLEKK 63

Query: 76  DRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQNSVPAITFLMAALLRI 135
           +RPA+ ++F++QFFLL LVGIT NQGFY+ GLDNTSPTFASA +N VPA++FLMAALL I
Sbjct: 64  ERPAMKISFLIQFFLLGLVGITLNQGFYIFGLDNTSPTFASATENVVPAVSFLMAALLGI 123

Query: 136 EQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTVQGTAETAGTMLMGPG 195
           E+V   RKDGIAKV+GTI  VAG+ VITLYKGPTIY P+                ++   
Sbjct: 124 EKVEWKRKDGIAKVVGTIVSVAGSLVITLYKGPTIYQPSLN--------------IVNQT 183

Query: 196 MFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARLSVTSFTCFFGLIQFL 255
           + P   + + K+WTLGC+ L+GHCL WS+W+VLQ+P+LKKYPAR S  S++CFF +IQF 
Sbjct: 184 IKPEEAEEENKNWTLGCLCLMGHCLCWSSWIVLQSPLLKKYPARFSFVSYSCFFAVIQFF 243

Query: 256 IIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTF 315
            I+  FERD + W   SG E ++++Y G+V S + FA+QI+ ++RGGP+FV+ Y P+QT 
Sbjct: 244 GISAYFERDLERWKIISGGELYALLYTGLVGSAMVFAIQIYVVERGGPLFVSAYLPLQTL 303

Query: 316 VVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILLER---AVIQSAPDHGS 375
           + A++A+ ALGE FYLGG+IGA+LI++GLYLV+ GKS E + L ++    +  +A D G 
Sbjct: 304 IAAVLATLALGEHFYLGGLIGAILIMSGLYLVVMGKSWENQALCQQQQHMISSAASDFGD 363

Query: 376 SRP--SGHIKSSITQPLL 389
                +   +S I+QPL+
Sbjct: 364 EEDYHNNKPRSPISQPLI 367

BLAST of Bhi04G000193 vs. TAIR 10
Match: AT3G18200.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 387.1 bits (993), Expect = 1.7e-107
Identity = 196/347 (56.48%), Postives = 257/347 (74.06%), Query Frame = 0

Query: 16  IPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVIALLLLLPFAYFIEKK 75
           + E+ +L  A++ LQF +AGFH+VSR ALN+G+SK+V+PVYRN++ALLL+ PFAYF EKK
Sbjct: 6   VSEKVKLVVALITLQFCFAGFHIVSRVALNIGVSKVVYPVYRNLLALLLIGPFAYFFEKK 65

Query: 76  DRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQNSVPAITFLMAALLRI 135
           +RP LT++ + QFF LAL+GITANQGFYLLGL   +PTFASA+QNSVPAITF+MA  LR+
Sbjct: 66  ERPPLTISLLAQFFFLALIGITANQGFYLLGLYYATPTFASAMQNSVPAITFIMACALRL 125

Query: 136 EQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTVQGTAETAGTMLMGPG 195
           E + L RK G+AKV+GT+  + GATVITLY+G  I+         +              
Sbjct: 126 EHIDLVRKHGVAKVLGTLVSIGGATVITLYRGFPIFDQGLNMQKEE-------------- 185

Query: 196 MFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARLSVTSFTCFFGLIQFL 255
               +G     S TLG +YL+GHCLSW+ W+VLQAPVLK+YPA+L++TSFTCFFGLIQFL
Sbjct: 186 ---VVGSDNSHSLTLGWLYLMGHCLSWAGWMVLQAPVLKQYPAKLTLTSFTCFFGLIQFL 245

Query: 256 IIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTF 315
           +IA+  E D   W+  S  E F+I+YAG++ASG+   +Q WCI + GPVFVAV+QP+QT 
Sbjct: 246 VIALFVETDLNNWIIVSWEELFTILYAGIIASGLVVYLQTWCIYKSGPVFVAVFQPLQTL 305

Query: 316 VVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILLERA 363
           +VA MA   LG++ Y GGI+GAV I+ GLYLVLWGK+EE+K+ LE +
Sbjct: 306 LVAAMAFLILGDQLYSGGIVGAVFIMLGLYLVLWGKNEERKLALEES 335

BLAST of Bhi04G000193 vs. TAIR 10
Match: AT3G18200.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 255.4 bits (651), Expect = 7.6e-68
Identity = 130/245 (53.06%), Postives = 171/245 (69.80%), Query Frame = 0

Query: 118 IQNSVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTT 177
           +QNSVPAITF+MA  LR+E + L RK G+AKV+GT+  + GATVITLY+G  I+      
Sbjct: 1   MQNSVPAITFIMACALRLEHIDLVRKHGVAKVLGTLVSIGGATVITLYRGFPIFDQGLNM 60

Query: 178 VTVQGTAETAGTMLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYP 237
              +                  +G     S TLG +YL+GHCLSW+ W+VLQAPVLK+YP
Sbjct: 61  QKEE-----------------VVGSDNSHSLTLGWLYLMGHCLSWAGWMVLQAPVLKQYP 120

Query: 238 ARLSVTSFTCFFGLIQFLIIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWC 297
           A+L++TSFTCFFGLIQFL+IA+  E D   W+  S  E F+I+YAG++ASG+   +Q WC
Sbjct: 121 AKLTLTSFTCFFGLIQFLVIALFVETDLNNWIIVSWEELFTILYAGIIASGLVVYLQTWC 180

Query: 298 IDRGGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKI 357
           I + GPVFVAV+QP+QT +VA MA   LG++ Y GGI+GAV I+ GLYLVLWGK+EE+K+
Sbjct: 181 IYKSGPVFVAVFQPLQTLLVAAMAFLILGDQLYSGGIVGAVFIMLGLYLVLWGKNEERKL 228

Query: 358 LLERA 363
            LE +
Sbjct: 241 ALEES 228

BLAST of Bhi04G000193 vs. ExPASy Swiss-Prot
Match: Q94AP3 (Protein WALLS ARE THIN 1 OS=Arabidopsis thaliana OX=3702 GN=WAT1 PE=1 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 4.5e-158
Identity = 292/399 (73.18%), Postives = 336/399 (84.21%), Query Frame = 0

Query: 1   MADTGSVSNKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60
           MAD  +  N+R    +PE+ QLH AML LQFGYAGFHVVSRAALNMGISKLVFPVYRN+I
Sbjct: 1   MAD--NTDNRRSLWGVPEKLQLHIAMLTLQFGYAGFHVVSRAALNMGISKLVFPVYRNII 60

Query: 61  ALLLLLPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120
           ALLLLLPFAYF+EKK+RPA+TLNF++QFF LAL+GITANQGFYLLGLDNTSPTFAS++QN
Sbjct: 61  ALLLLLPFAYFLEKKERPAITLNFLIQFFFLALIGITANQGFYLLGLDNTSPTFASSMQN 120

Query: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTV 180
           SVPAITFLMAALLRIE+VR+NR+DGI+K++GT  CVAGA+VITLYKGPTIY+PA+    +
Sbjct: 121 SVPAITFLMAALLRIEKVRINRRDGISKILGTALCVAGASVITLYKGPTIYTPAS---HL 180

Query: 181 QGTAETAGTMLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL 240
                T  + ++ P     LG+A  K+WTLGC+YLIGHCLSWS WLV QAPVLK YPARL
Sbjct: 181 HAHLLTTNSAVLAP-----LGNAAPKNWTLGCIYLIGHCLSWSGWLVFQAPVLKSYPARL 240

Query: 241 SVTSFTCFFGLIQFLIIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR 300
           SVTS+TCFFG+IQFLIIA   ERD+QAW+FHSG E F+I+YAG+VASGIAFAVQIWCIDR
Sbjct: 241 SVTSYTCFFGIIQFLIIAAFCERDSQAWVFHSGWELFTILYAGIVASGIAFAVQIWCIDR 300

Query: 301 GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKI-LL 360
           GGPVFVAVYQPVQT VVA+MAS ALGEEFYLGGIIGAVLII GLY VL+GKSEE+K   L
Sbjct: 301 GGPVFVAVYQPVQTLVVAIMASIALGEEFYLGGIIGAVLIIAGLYFVLYGKSEERKFAAL 360

Query: 361 ERAVIQSAPDHGSSR---PSGHIKSSITQPLLHPTAENV 396
           E+A IQS+ +HG  R       IKSSIT PLLH + +NV
Sbjct: 361 EKAAIQSSAEHGIERAPVSRNSIKSSITTPLLHQSTDNV 389

BLAST of Bhi04G000193 vs. ExPASy Swiss-Prot
Match: Q6J163 (Auxin-induced protein 5NG4 OS=Pinus taeda OX=3352 PE=2 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 4.1e-127
Identity = 248/401 (61.85%), Postives = 303/401 (75.56%), Query Frame = 0

Query: 8   SNKRMSCSI--PERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVIALLLL 67
           SN    C++   ER +LHAAMLALQFGYAGFH+VSRAALNMG+SK+VFPVYRN++AL+L+
Sbjct: 3   SNIMQRCNVFMSERVKLHAAMLALQFGYAGFHIVSRAALNMGVSKVVFPVYRNILALMLI 62

Query: 68  LPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQNSVPAI 127
            P AYF+EKK+RPALTL+F++QFFLLAL GIT       L +    PTFASAIQNSVPAI
Sbjct: 63  GPCAYFLEKKERPALTLSFLIQFFLLALCGITGQSRILSLRIVLHIPTFASAIQNSVPAI 122

Query: 128 TFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTVQGTAE 187
           TF+MAA LR+E+V ++R+DG+AK++GT+ CV+GAT+ITLYKGP I     T +       
Sbjct: 123 TFIMAAALRLEKVHISRRDGLAKIIGTVACVSGATIITLYKGPPI-----THIWRPNLEV 182

Query: 188 TAGTMLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARLSVTSF 247
           TA       G   S   AK ++WTLGC+YL+G+CL+WS W+VLQAPVLK+YPARLSVTSF
Sbjct: 183 TASYFKAFQGNDLS---AKSENWTLGCIYLLGNCLAWSGWIVLQAPVLKRYPARLSVTSF 242

Query: 248 TCFFGLIQFLIIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDRGGPVF 307
           TCFFG+IQFLIIA  FE D + W  HSG E F+I+YAG VASGIAF+VQIWCIDRGGPVF
Sbjct: 243 TCFFGVIQFLIIAAFFETDLEHWKIHSGGELFTILYAGFVASGIAFSVQIWCIDRGGPVF 302

Query: 308 VAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILLERA--- 367
           VAVYQPVQT  VA+MAS  LGE+FYLGGI GA+LII GLYLVLWGKSEEK++ L +A   
Sbjct: 303 VAVYQPVQTIAVAIMASIILGEQFYLGGIFGAILIIIGLYLVLWGKSEEKRLGLLQAKSS 362

Query: 368 -VIQSAPD----------HGSSRPSGHIKSSITQPLLHPTA 393
            V ++ PD          + S+    +  SS+TQPLL  T+
Sbjct: 363 MVPENQPDNMDQSATLIINSSNGIKPNTSSSLTQPLLLDTS 395

BLAST of Bhi04G000193 vs. ExPASy Swiss-Prot
Match: F4J9A3 (WAT1-related protein At3g53210 OS=Arabidopsis thaliana OX=3702 GN=At3g53210 PE=2 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 2.1e-110
Identity = 203/378 (53.70%), Postives = 275/378 (72.75%), Query Frame = 0

Query: 16  IPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVIALLLLLPFAYFIEKK 75
           IPER +LH AM+  Q GYAG HV+ R ALN+G+SKLVFP+YR ++A  +L P AYF+EKK
Sbjct: 4   IPERAKLHIAMVVFQTGYAGNHVIMRYALNLGVSKLVFPLYRTIVAFSVLAPSAYFLEKK 63

Query: 76  DRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQNSVPAITFLMAALLRI 135
           +RPA+ ++F++QFFLL LVGIT NQGFY+ GLDNTSPTFASA +N VPA++FLMAALL I
Sbjct: 64  ERPAMKISFLIQFFLLGLVGITLNQGFYIFGLDNTSPTFASATENVVPAVSFLMAALLGI 123

Query: 136 EQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTVQGTAETAGTMLMGPG 195
           E+V   RKDGIAKV+GTI  VAG+ VITLYKGPTIY P+                ++   
Sbjct: 124 EKVEWKRKDGIAKVVGTIVSVAGSLVITLYKGPTIYQPSLN--------------IVNQT 183

Query: 196 MFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARLSVTSFTCFFGLIQFL 255
           + P   + + K+WTLGC+ L+GHCL WS+W+VLQ+P+LKKYPAR S  S++CFF +IQF 
Sbjct: 184 IKPEEAEEENKNWTLGCLCLMGHCLCWSSWIVLQSPLLKKYPARFSFVSYSCFFAVIQFF 243

Query: 256 IIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTF 315
            I+  FERD + W   SG E ++++Y G+V S + FA+QI+ ++RGGP+FV+ Y P+QT 
Sbjct: 244 GISAYFERDLERWKIISGGELYALLYTGLVGSAMVFAIQIYVVERGGPLFVSAYLPLQTL 303

Query: 316 VVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILLER---AVIQSAPDHGS 375
           + A++A+ ALGE FYLGG+IGA+LI++GLYLV+ GKS E + L ++    +  +A D G 
Sbjct: 304 IAAVLATLALGEHFYLGGLIGAILIMSGLYLVVMGKSWENQALCQQQQHMISSAASDFGD 363

Query: 376 SRP--SGHIKSSITQPLL 389
                +   +S I+QPL+
Sbjct: 364 EEDYHNNKPRSPISQPLI 367

BLAST of Bhi04G000193 vs. ExPASy Swiss-Prot
Match: Q9LV20 (WAT1-related protein At3g18200 OS=Arabidopsis thaliana OX=3702 GN=At3g18200 PE=2 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 2.4e-106
Identity = 196/347 (56.48%), Postives = 257/347 (74.06%), Query Frame = 0

Query: 16  IPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVIALLLLLPFAYFIEKK 75
           + E+ +L  A++ LQF +AGFH+VSR ALN+G+SK+V+PVYRN++ALLL+ PFAYF EKK
Sbjct: 29  VSEKVKLVVALITLQFCFAGFHIVSRVALNIGVSKVVYPVYRNLLALLLIGPFAYFFEKK 88

Query: 76  DRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQNSVPAITFLMAALLRI 135
           +RP LT++ + QFF LAL+GITANQGFYLLGL   +PTFASA+QNSVPAITF+MA  LR+
Sbjct: 89  ERPPLTISLLAQFFFLALIGITANQGFYLLGLYYATPTFASAMQNSVPAITFIMACALRL 148

Query: 136 EQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTVQGTAETAGTMLMGPG 195
           E + L RK G+AKV+GT+  + GATVITLY+G  I+         +              
Sbjct: 149 EHIDLVRKHGVAKVLGTLVSIGGATVITLYRGFPIFDQGLNMQKEE-------------- 208

Query: 196 MFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARLSVTSFTCFFGLIQFL 255
               +G     S TLG +YL+GHCLSW+ W+VLQAPVLK+YPA+L++TSFTCFFGLIQFL
Sbjct: 209 ---VVGSDNSHSLTLGWLYLMGHCLSWAGWMVLQAPVLKQYPAKLTLTSFTCFFGLIQFL 268

Query: 256 IIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTF 315
           +IA+  E D   W+  S  E F+I+YAG++ASG+   +Q WCI + GPVFVAV+QP+QT 
Sbjct: 269 VIALFVETDLNNWIIVSWEELFTILYAGIIASGLVVYLQTWCIYKSGPVFVAVFQPLQTL 328

Query: 316 VVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILLERA 363
           +VA MA   LG++ Y GGI+GAV I+ GLYLVLWGK+EE+K+ LE +
Sbjct: 329 LVAAMAFLILGDQLYSGGIVGAVFIMLGLYLVLWGKNEERKLALEES 358

BLAST of Bhi04G000193 vs. ExPASy Swiss-Prot
Match: Q5PP32 (WAT1-related protein At3g45870 OS=Arabidopsis thaliana OX=3702 GN=At3g45870 PE=2 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.6e-65
Identity = 152/383 (39.69%), Postives = 228/383 (59.53%), Query Frame = 0

Query: 18  ERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVIALLLLLPFAYFIEKKDR 77
           E ++ H AM+ +Q    G+HV+++ ALN+G+++LVF V+R++IAL +L P AY  +K+ R
Sbjct: 8   EAWKAHVAMIGVQLFNGGYHVITKVALNVGVNQLVFCVFRDLIALSILAPLAYIRDKRTR 67

Query: 78  PALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQNSVPAITFLMAALLRIEQ 137
           P L   F+L FF L L GI  NQ  +L+GL+ T+PT+A+AIQ S+P  TF++A ++  E+
Sbjct: 68  PPLNRQFLLAFFFLGLTGIFGNQLLFLIGLNYTNPTYAAAIQPSIPVFTFILALIMGTER 127

Query: 138 VRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTVQGTA---ETAGTMLMGP 197
           + L + +G AKV GT+ CVAGA ++ L++G  ++          G +   ET+G  +   
Sbjct: 128 LNLFKLEGQAKVGGTLICVAGAVLMVLFRGLALFGETEAESLGHGESRHTETSGHFM--S 187

Query: 198 GMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARLSVTSFTCFFGLIQF 257
           G F  LG      W LG + LIG+C   +A+L +QAPVLKKYPA LSVT+++ FFG +  
Sbjct: 188 GFFNGLG-----RWNLGVLCLIGNCTCMAAFLAIQAPVLKKYPANLSVTAYSYFFGTMFM 247

Query: 258 LIIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDRGGPVFVAVYQPVQT 317
           +  A     ++  W   + +E F++VYAGV+AS + + +  W     GP  VA+Y P+Q 
Sbjct: 248 VTSAFFMTNESTNWSL-TRSEFFAVVYAGVIASALNYGLLTWSNKILGPSLVALYNPLQP 307

Query: 318 FVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILLERAVI-----QSAP- 377
              A ++   LG   YLG I+G   II GLY V W   +EKK     AVI     ++ P 
Sbjct: 308 AASAFLSRIFLGSPIYLGSILGGCAIIAGLYSVTWASYKEKKAAAAMAVIPITSKEAEPL 367

Query: 378 ---DHGSSRPSGHI--KSSITQP 387
              DH  ++P GH+  KS I+ P
Sbjct: 368 IYKDH-KNKPIGHLFTKSPISSP 381

BLAST of Bhi04G000193 vs. NCBI nr
Match: XP_038884865.1 (protein WALLS ARE THIN 1-like [Benincasa hispida])

HSP 1 Score: 765.0 bits (1974), Expect = 3.2e-217
Identity = 395/395 (100.00%), Postives = 395/395 (100.00%), Query Frame = 0

Query: 1   MADTGSVSNKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60
           MADTGSVSNKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI
Sbjct: 1   MADTGSVSNKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60

Query: 61  ALLLLLPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120
           ALLLLLPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN
Sbjct: 61  ALLLLLPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120

Query: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTV 180
           SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTV
Sbjct: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTV 180

Query: 181 QGTAETAGTMLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL 240
           QGTAETAGTMLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL
Sbjct: 181 QGTAETAGTMLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL 240

Query: 241 SVTSFTCFFGLIQFLIIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR 300
           SVTSFTCFFGLIQFLIIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR
Sbjct: 241 SVTSFTCFFGLIQFLIIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR 300

Query: 301 GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILLE 360
           GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILLE
Sbjct: 301 GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILLE 360

Query: 361 RAVIQSAPDHGSSRPSGHIKSSITQPLLHPTAENV 396
           RAVIQSAPDHGSSRPSGHIKSSITQPLLHPTAENV
Sbjct: 361 RAVIQSAPDHGSSRPSGHIKSSITQPLLHPTAENV 395

BLAST of Bhi04G000193 vs. NCBI nr
Match: XP_004152669.1 (protein WALLS ARE THIN 1 [Cucumis sativus] >KGN62642.1 hypothetical protein Csa_022013 [Cucumis sativus])

HSP 1 Score: 728.8 bits (1880), Expect = 2.5e-206
Identity = 377/396 (95.20%), Postives = 385/396 (97.22%), Query Frame = 0

Query: 1   MADTGSVSNKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60
           MAD+GS S+KRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI
Sbjct: 1   MADSGSASDKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60

Query: 61  ALLLLLPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120
           ALLLLLPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFAS IQN
Sbjct: 61  ALLLLLPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASCIQN 120

Query: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTV 180
           SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSP+TTTVT+
Sbjct: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPSTTTVTL 180

Query: 181 QGTAETAGTM-LMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPAR 240
           QG AET   M L GPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPAR
Sbjct: 181 QGMAETTAAMTLQGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPAR 240

Query: 241 LSVTSFTCFFGLIQFLIIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCID 300
           LSVTSFTCFFGLIQFLIIA+AFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCID
Sbjct: 241 LSVTSFTCFFGLIQFLIIAVAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCID 300

Query: 301 RGGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILL 360
           RGGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILL
Sbjct: 301 RGGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILL 360

Query: 361 ERAVIQSAPDHGSSRPSGHIKSSITQPLLHPTAENV 396
           ER++IQ+APDHGSSR SG IK SITQPLLHPTAENV
Sbjct: 361 ERSMIQTAPDHGSSRISGQIKPSITQPLLHPTAENV 396

BLAST of Bhi04G000193 vs. NCBI nr
Match: XP_022997349.1 (protein WALLS ARE THIN 1-like [Cucurbita maxima])

HSP 1 Score: 726.9 bits (1875), Expect = 9.6e-206
Identity = 376/395 (95.19%), Postives = 385/395 (97.47%), Query Frame = 0

Query: 1   MADTGSVSNKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60
           MADTGSVS++RMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI
Sbjct: 1   MADTGSVSDRRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60

Query: 61  ALLLLLPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120
           ALLLLLPFAYF+EKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN
Sbjct: 61  ALLLLLPFAYFLEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120

Query: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTV 180
           SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPA TT+TV
Sbjct: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPA-TTMTV 180

Query: 181 QGTAETAGTMLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL 240
           QGTAETAG MLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL
Sbjct: 181 QGTAETAG-MLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL 240

Query: 241 SVTSFTCFFGLIQFLIIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR 300
           SVTSFTCFFGLIQF IIA  FERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR
Sbjct: 241 SVTSFTCFFGLIQFFIIAAVFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR 300

Query: 301 GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILLE 360
           GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLII+GLYLVLWGKSEEKK LLE
Sbjct: 301 GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIISGLYLVLWGKSEEKKFLLE 360

Query: 361 RAVIQSAPDHGSSRPSGHIKSSITQPLLHPTAENV 396
           RAVIQS PDHG+ RPSGHIK S+ QPLLHPTA++V
Sbjct: 361 RAVIQSVPDHGTRRPSGHIKPSLNQPLLHPTADSV 393

BLAST of Bhi04G000193 vs. NCBI nr
Match: XP_022961629.1 (protein WALLS ARE THIN 1-like [Cucurbita moschata] >KAG7029410.1 Protein WALLS ARE THIN 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 726.9 bits (1875), Expect = 9.6e-206
Identity = 376/395 (95.19%), Postives = 385/395 (97.47%), Query Frame = 0

Query: 1   MADTGSVSNKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60
           MADTGSVS++RMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI
Sbjct: 1   MADTGSVSDRRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60

Query: 61  ALLLLLPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120
           ALLLLLPFAYF+EKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN
Sbjct: 61  ALLLLLPFAYFLEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120

Query: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTV 180
           SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPA TTVTV
Sbjct: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPA-TTVTV 180

Query: 181 QGTAETAGTMLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL 240
           +GTAETAG MLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL
Sbjct: 181 EGTAETAG-MLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL 240

Query: 241 SVTSFTCFFGLIQFLIIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR 300
           SVTSFTCFFGLIQF IIA  FERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR
Sbjct: 241 SVTSFTCFFGLIQFFIIAAVFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR 300

Query: 301 GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILLE 360
           GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLII+GLYLVLWGKSEEKK LLE
Sbjct: 301 GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIISGLYLVLWGKSEEKKFLLE 360

Query: 361 RAVIQSAPDHGSSRPSGHIKSSITQPLLHPTAENV 396
           RAVIQS PDHG+ RPSGHIK S+ QPLLHPTA++V
Sbjct: 361 RAVIQSVPDHGTRRPSGHIKPSLNQPLLHPTADSV 393

BLAST of Bhi04G000193 vs. NCBI nr
Match: XP_023545575.1 (protein WALLS ARE THIN 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 724.9 bits (1870), Expect = 3.6e-205
Identity = 375/395 (94.94%), Postives = 384/395 (97.22%), Query Frame = 0

Query: 1   MADTGSVSNKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60
           MADTGSVS++RMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI
Sbjct: 1   MADTGSVSDRRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60

Query: 61  ALLLLLPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120
           ALLLLLPFAYF+EKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN
Sbjct: 61  ALLLLLPFAYFLEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120

Query: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTV 180
           SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPA T VTV
Sbjct: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPA-TNVTV 180

Query: 181 QGTAETAGTMLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL 240
           QGTAETAG MLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL
Sbjct: 181 QGTAETAG-MLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL 240

Query: 241 SVTSFTCFFGLIQFLIIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR 300
           SVTS+TCFFGLIQF IIA  FERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR
Sbjct: 241 SVTSYTCFFGLIQFFIIAAVFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR 300

Query: 301 GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILLE 360
           GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLII+GLYLVLWGKSEEKK LLE
Sbjct: 301 GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIISGLYLVLWGKSEEKKFLLE 360

Query: 361 RAVIQSAPDHGSSRPSGHIKSSITQPLLHPTAENV 396
           RAVIQS PDHG+ RPSGHIK S+ QPLLHPTA++V
Sbjct: 361 RAVIQSVPDHGTRRPSGHIKPSLNQPLLHPTADSV 393

BLAST of Bhi04G000193 vs. ExPASy TrEMBL
Match: A0A0A0LPD6 (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_2G363030 PE=3 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 1.2e-206
Identity = 377/396 (95.20%), Postives = 385/396 (97.22%), Query Frame = 0

Query: 1   MADTGSVSNKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60
           MAD+GS S+KRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI
Sbjct: 1   MADSGSASDKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60

Query: 61  ALLLLLPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120
           ALLLLLPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFAS IQN
Sbjct: 61  ALLLLLPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASCIQN 120

Query: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTV 180
           SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSP+TTTVT+
Sbjct: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPSTTTVTL 180

Query: 181 QGTAETAGTM-LMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPAR 240
           QG AET   M L GPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPAR
Sbjct: 181 QGMAETTAAMTLQGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPAR 240

Query: 241 LSVTSFTCFFGLIQFLIIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCID 300
           LSVTSFTCFFGLIQFLIIA+AFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCID
Sbjct: 241 LSVTSFTCFFGLIQFLIIAVAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCID 300

Query: 301 RGGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILL 360
           RGGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILL
Sbjct: 301 RGGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILL 360

Query: 361 ERAVIQSAPDHGSSRPSGHIKSSITQPLLHPTAENV 396
           ER++IQ+APDHGSSR SG IK SITQPLLHPTAENV
Sbjct: 361 ERSMIQTAPDHGSSRISGQIKPSITQPLLHPTAENV 396

BLAST of Bhi04G000193 vs. ExPASy TrEMBL
Match: A0A6J1KB58 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111492292 PE=3 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 4.6e-206
Identity = 376/395 (95.19%), Postives = 385/395 (97.47%), Query Frame = 0

Query: 1   MADTGSVSNKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60
           MADTGSVS++RMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI
Sbjct: 1   MADTGSVSDRRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60

Query: 61  ALLLLLPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120
           ALLLLLPFAYF+EKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN
Sbjct: 61  ALLLLLPFAYFLEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120

Query: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTV 180
           SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPA TT+TV
Sbjct: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPA-TTMTV 180

Query: 181 QGTAETAGTMLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL 240
           QGTAETAG MLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL
Sbjct: 181 QGTAETAG-MLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL 240

Query: 241 SVTSFTCFFGLIQFLIIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR 300
           SVTSFTCFFGLIQF IIA  FERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR
Sbjct: 241 SVTSFTCFFGLIQFFIIAAVFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR 300

Query: 301 GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILLE 360
           GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLII+GLYLVLWGKSEEKK LLE
Sbjct: 301 GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIISGLYLVLWGKSEEKKFLLE 360

Query: 361 RAVIQSAPDHGSSRPSGHIKSSITQPLLHPTAENV 396
           RAVIQS PDHG+ RPSGHIK S+ QPLLHPTA++V
Sbjct: 361 RAVIQSVPDHGTRRPSGHIKPSLNQPLLHPTADSV 393

BLAST of Bhi04G000193 vs. ExPASy TrEMBL
Match: A0A6J1HEL9 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111462346 PE=3 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 4.6e-206
Identity = 376/395 (95.19%), Postives = 385/395 (97.47%), Query Frame = 0

Query: 1   MADTGSVSNKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60
           MADTGSVS++RMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI
Sbjct: 1   MADTGSVSDRRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60

Query: 61  ALLLLLPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120
           ALLLLLPFAYF+EKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN
Sbjct: 61  ALLLLLPFAYFLEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120

Query: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTV 180
           SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPA TTVTV
Sbjct: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPA-TTVTV 180

Query: 181 QGTAETAGTMLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL 240
           +GTAETAG MLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL
Sbjct: 181 EGTAETAG-MLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL 240

Query: 241 SVTSFTCFFGLIQFLIIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR 300
           SVTSFTCFFGLIQF IIA  FERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR
Sbjct: 241 SVTSFTCFFGLIQFFIIAAVFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR 300

Query: 301 GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILLE 360
           GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLII+GLYLVLWGKSEEKK LLE
Sbjct: 301 GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIISGLYLVLWGKSEEKKFLLE 360

Query: 361 RAVIQSAPDHGSSRPSGHIKSSITQPLLHPTAENV 396
           RAVIQS PDHG+ RPSGHIK S+ QPLLHPTA++V
Sbjct: 361 RAVIQSVPDHGTRRPSGHIKPSLNQPLLHPTADSV 393

BLAST of Bhi04G000193 vs. ExPASy TrEMBL
Match: A0A6J1KNR5 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111496234 PE=3 SV=1)

HSP 1 Score: 719.2 bits (1855), Expect = 9.7e-204
Identity = 373/395 (94.43%), Postives = 383/395 (96.96%), Query Frame = 0

Query: 1   MADTGSVSNKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60
           MADTGSVSNKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI
Sbjct: 1   MADTGSVSNKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60

Query: 61  ALLLLLPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120
           ALLLLLPFAYF+EKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN
Sbjct: 61  ALLLLLPFAYFMEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120

Query: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTV 180
           SVPAITFLMA LLRIEQVRLNRKDGIAKV+GT+CCVAGATVITLYKGPT+YSPA TTVTV
Sbjct: 121 SVPAITFLMAVLLRIEQVRLNRKDGIAKVVGTLCCVAGATVITLYKGPTVYSPA-TTVTV 180

Query: 181 QGTAETAGTMLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL 240
           Q TAE+AG MLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL
Sbjct: 181 QSTAESAG-MLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL 240

Query: 241 SVTSFTCFFGLIQFLIIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR 300
           SVTSFTCFFGL+QF IIA AFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR
Sbjct: 241 SVTSFTCFFGLVQFFIIAAAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR 300

Query: 301 GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILLE 360
           GGPVFVAVYQPVQTFVVALMAS ALGEEFYLGGIIGAVLII+GLYLVLWGKSEEKK+LLE
Sbjct: 301 GGPVFVAVYQPVQTFVVALMASLALGEEFYLGGIIGAVLIISGLYLVLWGKSEEKKLLLE 360

Query: 361 RAVIQSAPDHGSSRPSGHIKSSITQPLLHPTAENV 396
           RAVIQSA DH +SR SGHIK S+ QPLLHPTAENV
Sbjct: 361 RAVIQSAQDHVTSRTSGHIKPSLNQPLLHPTAENV 393

BLAST of Bhi04G000193 vs. ExPASy TrEMBL
Match: A0A6J1GHE4 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111454189 PE=3 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 9.1e-202
Identity = 370/395 (93.67%), Postives = 380/395 (96.20%), Query Frame = 0

Query: 1   MADTGSVSNKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60
           MAD GSVSNKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI
Sbjct: 1   MADIGSVSNKRMSCSIPERFQLHAAMLALQFGYAGFHVVSRAALNMGISKLVFPVYRNVI 60

Query: 61  ALLLLLPFAYFIEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120
           ALLLLLPFAYF+EKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN
Sbjct: 61  ALLLLLPFAYFMEKKDRPALTLNFVLQFFLLALVGITANQGFYLLGLDNTSPTFASAIQN 120

Query: 121 SVPAITFLMAALLRIEQVRLNRKDGIAKVMGTICCVAGATVITLYKGPTIYSPATTTVTV 180
           SVPAITFLMA LLRIEQVRLNRKDGIAKV+GT+CCVAGATVITLYKGPT+YSPA TTVTV
Sbjct: 121 SVPAITFLMAVLLRIEQVRLNRKDGIAKVVGTLCCVAGATVITLYKGPTVYSPA-TTVTV 180

Query: 181 QGTAETAGTMLMGPGMFPSLGDAKGKSWTLGCVYLIGHCLSWSAWLVLQAPVLKKYPARL 240
             TAETAG MLMGPGMFPSLGDAKGKSWTLGC+YLIGHCLSWSAWLVLQAPVLKKYPARL
Sbjct: 181 HSTAETAG-MLMGPGMFPSLGDAKGKSWTLGCMYLIGHCLSWSAWLVLQAPVLKKYPARL 240

Query: 241 SVTSFTCFFGLIQFLIIAIAFERDAQAWLFHSGAEAFSIVYAGVVASGIAFAVQIWCIDR 300
           SVTSFTCFFGLIQF IIA AFERDAQAWLFHS AEAFSIVYAGVVASGIAFAVQIWCIDR
Sbjct: 241 SVTSFTCFFGLIQFFIIAAAFERDAQAWLFHSDAEAFSIVYAGVVASGIAFAVQIWCIDR 300

Query: 301 GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIITGLYLVLWGKSEEKKILLE 360
           GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLII+GLYLVLWGKSEE+K+LLE
Sbjct: 301 GGPVFVAVYQPVQTFVVALMASFALGEEFYLGGIIGAVLIISGLYLVLWGKSEERKLLLE 360

Query: 361 RAVIQSAPDHGSSRPSGHIKSSITQPLLHPTAENV 396
            AVIQS PDH +SR SGHIK S+ QPLLHPTAENV
Sbjct: 361 WAVIQSTPDHVTSRTSGHIKPSLNQPLLHPTAENV 393

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G75500.13.2e-15973.18Walls Are Thin 1 [more]
AT1G75500.23.2e-15973.18Walls Are Thin 1 [more]
AT3G53210.11.5e-11153.70nodulin MtN21 /EamA-like transporter family protein [more]
AT3G18200.11.7e-10756.48nodulin MtN21 /EamA-like transporter family protein [more]
AT3G18200.27.6e-6853.06nodulin MtN21 /EamA-like transporter family protein [more]
Match NameE-valueIdentityDescription
Q94AP34.5e-15873.18Protein WALLS ARE THIN 1 OS=Arabidopsis thaliana OX=3702 GN=WAT1 PE=1 SV=1[more]
Q6J1634.1e-12761.85Auxin-induced protein 5NG4 OS=Pinus taeda OX=3352 PE=2 SV=1[more]
F4J9A32.1e-11053.70WAT1-related protein At3g53210 OS=Arabidopsis thaliana OX=3702 GN=At3g53210 PE=2... [more]
Q9LV202.4e-10656.48WAT1-related protein At3g18200 OS=Arabidopsis thaliana OX=3702 GN=At3g18200 PE=2... [more]
Q5PP321.6e-6539.69WAT1-related protein At3g45870 OS=Arabidopsis thaliana OX=3702 GN=At3g45870 PE=2... [more]
Match NameE-valueIdentityDescription
XP_038884865.13.2e-217100.00protein WALLS ARE THIN 1-like [Benincasa hispida][more]
XP_004152669.12.5e-20695.20protein WALLS ARE THIN 1 [Cucumis sativus] >KGN62642.1 hypothetical protein Csa_... [more]
XP_022997349.19.6e-20695.19protein WALLS ARE THIN 1-like [Cucurbita maxima][more]
XP_022961629.19.6e-20695.19protein WALLS ARE THIN 1-like [Cucurbita moschata] >KAG7029410.1 Protein WALLS A... [more]
XP_023545575.13.6e-20594.94protein WALLS ARE THIN 1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0LPD61.2e-20695.20WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_2G363030 PE=3 SV=1[more]
A0A6J1KB584.6e-20695.19WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111492292 PE=3 SV=1[more]
A0A6J1HEL94.6e-20695.19WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111462346 PE=3 SV=1[more]
A0A6J1KNR59.7e-20494.43WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111496234 PE=3 SV=1[more]
A0A6J1GHE49.1e-20293.67WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111454189 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 210..348
e-value: 2.4E-14
score: 53.7
coord: 26..163
e-value: 2.5E-14
score: 53.6
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 14..373
NoneNo IPR availablePANTHERPTHR31218:SF338WAT1-RELATED PROTEINcoord: 14..373
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 253..352
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 56..166

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi04M000193Bhi04M000193mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity