Bhi03G001900 (gene) Wax gourd (B227) v1

Overview


Name	Bhi03G001900
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Protein kinase domain-containing protein
Location	chr3: 52450544 .. 52451253 (+)
RNA-Seq Expression	Bhi03G001900
Synteny	Bhi03G001900

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAATCCCTTTATAAATTCATCTTTATCTCCAGATTGTGACCAATCTCTTAGGCCGAAGAGGATGATTACGAAATTCCCAAACCCTAACAACAGCAATGGAGTCCAAAGTCAAACGCAAATCGACGGTTTCGACAACTCTCATGGTTCCTTTTGGTTTCGTGGTCGTCTTCTCGGCAAAGGTAGCTTCGGCTCTGTTTTCTTAGCCCAAATTAACACAGACTTTTTTATCGATTCACATTATGGTAAACTTCCTCTTGGTAAATATATTGCTGTCAAATCTCCTTCTTCAAAGATTCCTCCTCCCTTCGATTTGAGAAGAAGGTACTTGATGCTTTCAAGTCTTGCTCTAATGTAGTTCAATGTTATGGACATGAAATTACAAATACTGTATCTGGCAATTACATTTACAATTTACTTCTCGAGTATTGTTCTGGAGGTAGTTTGTACGATCGTATTACGAAATTCGGACCCAATGGCTTGTCGGAATTGGAAGTTCGTCGATATACCAGAGACATTGTCTATGGAATTTATTCTATTCATGGACTTGGATATATTCATTGTGATATTAAACCAGAAAACATACTTCTGATTCCGAGTTTTGATCATGGAGGATTTTTGGTTGCGAAAATAGCTGATTTTGGATTGGCAATGCAGATGACCAATGTAGTGAATATTGATGACGATAATAGTACTAGGGGAACTTAA

mRNA sequence

ATGAAGAATCCCTTTATAAATTCATCTTTATCTCCAGATTGTGACCAATCTCTTAGGCCGAAGAGGATGATTACGAAATTCCCAAACCCTAACAACAGCAATGGAGTCCAAAGTCAAACGCAAATCGACGGTTTCGACAACTCTCATGGTTCCTTTTGGTTTCGTGGTCGTCTTCTCGGCAAAGATTCCTCCTCCCTTCGATTTGAGAAGAAGGTACTTGATGCTTTCAAGTCTTGCTCTAATGTAGTTCAATGTTATGGACATGAAATTACAAATACTGTATCTGGCAATTACATTTACAATTTACTTCTCGAGTATTGTTCTGGAGGTAGTTTGTACGATCGTATTACGAAATTCGGACCCAATGGCTTGTCGGAATTGGAAGTTCGTCGATATACCAGAGACATTGTCTATGGAATTTATTCTATTCATGGACTTGGATATATTCATTGTGATATTAAACCAGAAAACATACTTCTGATTCCGAGTTTTGATCATGGAGGATTTTTGGTTGCGAAAATAGCTGATTTTGGATTGGCAATGCAGATGACCAATGTAGTGAATATTGATGACGATAATAGTACTAGGGGAACTTAA

Coding sequence (CDS)

ATGAAGAATCCCTTTATAAATTCATCTTTATCTCCAGATTGTGACCAATCTCTTAGGCCGAAGAGGATGATTACGAAATTCCCAAACCCTAACAACAGCAATGGAGTCCAAAGTCAAACGCAAATCGACGGTTTCGACAACTCTCATGGTTCCTTTTGGTTTCGTGGTCGTCTTCTCGGCAAAGATTCCTCCTCCCTTCGATTTGAGAAGAAGGTACTTGATGCTTTCAAGTCTTGCTCTAATGTAGTTCAATGTTATGGACATGAAATTACAAATACTGTATCTGGCAATTACATTTACAATTTACTTCTCGAGTATTGTTCTGGAGGTAGTTTGTACGATCGTATTACGAAATTCGGACCCAATGGCTTGTCGGAATTGGAAGTTCGTCGATATACCAGAGACATTGTCTATGGAATTTATTCTATTCATGGACTTGGATATATTCATTGTGATATTAAACCAGAAAACATACTTCTGATTCCGAGTTTTGATCATGGAGGATTTTTGGTTGCGAAAATAGCTGATTTTGGATTGGCAATGCAGATGACCAATGTAGTGAATATTGATGACGATAATAGTACTAGGGGAACTTAA

Protein sequence

MKNPFINSSLSPDCDQSLRPKRMITKFPNPNNSNGVQSQTQIDGFDNSHGSFWFRGRLLGKDSSSLRFEKKVLDAFKSCSNVVQCYGHEITNTVSGNYIYNLLLEYCSGGSLYDRITKFGPNGLSELEVRRYTRDIVYGIYSIHGLGYIHCDIKPENILLIPSFDHGGFLVAKIADFGLAMQMTNVVNIDDDNSTRGT

Homology

[Close]

BLAST of Bhi03G001900 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 112.1 bits (279), Expect = 5.2e-25
Identity = 62/128 (48.44%), Postives = 79/128 (61.72%), Query Frame = 0


Query: 63  SSSLRFEKKVLDAFKSCSNVVQCYGHEITNTVSGNYIYNLLLEYCSGGSLYDRITKFGPN 122
           S++LR E+ VLD    CS +V+C+G E     +G  IYNL LEY SGGSL DRI   G  
Sbjct: 44  SAALRNERDVLDDLGDCSEIVRCFG-EGRTVENGEEIYNLFLEYASGGSLADRIKSSG-E 103

Query: 123 GLSELEVRRYTRDIVYGIYSIHGLGYIHCDIKPENILLIPSFDHGGFLVAKIADFGLAMQ 182
            L E EVRR+TR IV G+  IHG G+ HCDIK EN+L+    D       KI+DFGLA +
Sbjct: 104 ALPEFEVRRFTRSIVKGLCHIHGNGFTHCDIKLENVLVFGDGD------VKISDFGLAKR 163

Query: 183 MTNVVNID 191
            +  V ++
Sbjct: 164 RSGEVCVE 163

[Close]

BLAST of Bhi03G001900 vs. TAIR 10
Match: AT5G27510.1 (Protein kinase superfamily protein )

HSP 1 Score: 109.4 bits (272), Expect = 3.4e-24
Identity = 58/154 (37.66%), Postives = 84/154 (54.55%), Query Frame = 0


Query: 35  GVQSQTQIDGFDNSHGSFWFRGRLLGKDSSSLRFEKKVLDAFKSCSNVVQCYGHEITNTV 94
           G  S   +  +  S GS +        D +SL  E  +L   K C  ++QC+G+++    
Sbjct: 14  GAYSFVDLFKYTKSDGSSFHAAVKSSDDENSLLKEFHILSELKGCPRIIQCFGNDLEEGF 73

Query: 95  --SGNYIYNLLLEYCSGGSLYDRITKFGPNGLSELEVRRYTRDIVYGIYSIHGLGYIHCD 154
              GN +Y LLLEY S GSL D +       L +L +R +TR I+ G+ SIH  GY+HCD
Sbjct: 74  DDKGNRVYKLLLEYASEGSLSDFMNNCVDRKLPDLMIRDFTRMILQGLVSIHSHGYVHCD 133

Query: 155 IKPENILLIPSFDHGGFLVAKIADFGLAMQMTNV 187
           +KPEN+L+ P    G     KI+DFGL++Q+  V
Sbjct: 134 LKPENVLVFPC---GDSYEVKISDFGLSLQVGEV 164

[Close]

BLAST of Bhi03G001900 vs. TAIR 10
Match: AT2G05060.1 (Protein kinase superfamily protein )

HSP 1 Score: 109.0 bits (271), Expect = 4.4e-24
Identity = 53/120 (44.17%), Postives = 79/120 (65.83%), Query Frame = 0


Query: 63  SSSLRFEKKVLDAFKSCSNVVQCYGHEITNTVS--GNYIYNLLLEYCSGGSLYDRITKFG 122
           + SL  E ++L  FK CS +VQCYG ++  T++  G+  + + +EY SGGSL   +++F 
Sbjct: 54  ADSLLKEFQILSEFKGCSRIVQCYGTKVQETINEEGDVEFTIPMEYASGGSLRHFMSRFK 113

Query: 123 PNGLSELEVRRYTRDIVYGIYSIHGLGYIHCDIKPENILLIPSFDHGGFLVAKIADFGLA 181
              L +  +RR+TR I+ G+  IHG GY+HCD+KPENIL+ PSF+       KI+DFGL+
Sbjct: 114 DMKLPDALIRRFTRMILEGLAVIHGHGYVHCDLKPENILVFPSFE------LKISDFGLS 167

[Close]

BLAST of Bhi03G001900 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 104.0 bits (258), Expect = 1.4e-22
Identity = 56/121 (46.28%), Postives = 75/121 (61.98%), Query Frame = 0


Query: 63  SSSLRFEKKVLDAFKSCSNVVQCYGHEITNTVSGNYIYNLLLEYCSGGSLYDRITKFGPN 122
           ++SL  EK VLD+   C  +++CYG + T   +G  ++NLLLEY S GSL   + K G  
Sbjct: 43  AASLSNEKSVLDSLGDCPEIIRCYGEDST-VENGEEMHNLLLEYASRGSLASYMKKLGGE 102

Query: 123 GLSELEVRRYTRDIVYGIYSIHGLGYIHCDIKPENILLIPSFDHGGFLVAKIADFGLAMQ 182
           GL E  VRR+T  ++ G+  IH  G+ HCDIK  NILL   F+ G     KIADFGLAM+
Sbjct: 103 GLPESTVRRHTGSVLRGLRHIHAKGFAHCDIKLANILL---FNDGS---VKIADFGLAMR 156

Query: 183 M 184
           +
Sbjct: 163 V 156

[Close]

BLAST of Bhi03G001900 vs. TAIR 10
Match: AT3G45790.1 (Protein kinase superfamily protein )

HSP 1 Score: 103.6 bits (257), Expect = 1.9e-22
Identity = 62/127 (48.82%), Postives = 80/127 (62.99%), Query Frame = 0


Query: 63  SSSLRFEKKVLDAFKSCSNVVQCYGHEIT--NTVSG--NYIYNLLLEYCSGGSLYDRITK 122
           +SSL  E+++L    S   +V+CYGHEI    T+ G     YNL+LEYCSG SL+D +  
Sbjct: 134 ASSLMDEERILTRLSS-PFIVRCYGHEIAREETLFGGERTNYNLILEYCSGKSLFDLVNS 193

Query: 123 FGPNGLSELEVRRYTRDIVYGIYSIHGLGYIHCDIKPENILLIPSFDH---GGFLVAKIA 182
               GLSE +V+   RDI+YG+  IH    IHCDIKPENILL P  +     G+ VAKI 
Sbjct: 194 -NLGGLSEKDVKLLARDILYGLDYIHRANIIHCDIKPENILLAPVENRIRPNGY-VAKIG 253

[Close]

BLAST of Bhi03G001900 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 2.0e-21
Identity = 56/121 (46.28%), Postives = 75/121 (61.98%), Query Frame = 0


Query: 63  SSSLRFEKKVLDAFKSCSNVVQCYGHEITNTVSGNYIYNLLLEYCSGGSLYDRITKFGPN 122
           ++SL  EK VLD+   C  +++CYG + T   +G  ++NLLLEY S GSL   + K G  
Sbjct: 43  AASLSNEKSVLDSLGDCPEIIRCYGEDST-VENGEEMHNLLLEYASRGSLASYMKKLGGE 102

Query: 123 GLSELEVRRYTRDIVYGIYSIHGLGYIHCDIKPENILLIPSFDHGGFLVAKIADFGLAMQ 182
           GL E  VRR+T  ++ G+  IH  G+ HCDIK  NILL   F+ G     KIADFGLAM+
Sbjct: 103 GLPESTVRRHTGSVLRGLRHIHAKGFAHCDIKLANILL---FNDGS---VKIADFGLAMR 156

Query: 183 M 184
           +
Sbjct: 163 V 156

[Close]

BLAST of Bhi03G001900 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.5e-13
Identity = 52/160 (32.50%), Postives = 76/160 (47.50%), Query Frame = 0


Query: 53  WFRGRLLGKDSSS--------------------------LRFEKKVLDAFKSCSNVVQCY 112
           W RGR+LG+ S++                          L+ E K+L +  S   V+   
Sbjct: 3   WTRGRILGRGSTATVYAAAGHNSDEILAVKSSEVHRSEFLQREAKILSSLSS-PYVIGYR 62

Query: 113 GHEITNTVSGNYIYNLLLEYCSGGSLYDRITKFGPNGLSELEVRRYTRDIVYGIYSIHGL 172
           G E     +G  +YNLL+EY   G+L D   K G   + E  V +YTRDI+ G+  IH  
Sbjct: 63  GSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDG-GRVDETRVVKYTRDILKGLEYIHSK 122

Query: 173 GYIHCDIKPENILLIPSFDHGGFLVAKIADFGLAMQMTNV 187
           G +HCD+K  N+++    +      AKIADFG A ++  V
Sbjct: 123 GIVHCDVKGSNVVISEKGE------AKIADFGCAKRVDPV 154

[Close]

BLAST of Bhi03G001900 vs. ExPASy Swiss-Prot
Match: O35280 (Serine/threonine-protein kinase Chk1 OS=Mus musculus OX=10090 GN=Chek1 PE=1 SV=2)

HSP 1 Score: 75.5 bits (184), Expect = 7.6e-13
Identity = 45/103 (43.69%), Postives = 59/103 (57.28%), Query Frame = 0


Query: 78  SCSNVVQCYGHEITNTVSGNYIYNLLLEYCSGGSLYDRITKFGPNGLSELEVRRYTRDIV 137
           S  NVV+ YGH         +I  L LEYCSGG L+DRI      G+ E + +R+   ++
Sbjct: 63  SHENVVKFYGHR-----REGHIQYLFLEYCSGGELFDRIEP--DIGMPEQDAQRFFHQLM 122

Query: 138 YGIYSIHGLGYIHCDIKPENILLIPSFDHGGFLVAKIADFGLA 181
            G+  +HG+G  H DIKPEN+LL    D    L  KI+DFGLA
Sbjct: 123 AGVVYLHGIGITHRDIKPENLLL----DERDNL--KISDFGLA 152

[Close]

BLAST of Bhi03G001900 vs. ExPASy Swiss-Prot
Match: Q91ZN7 (Serine/threonine-protein kinase Chk1 OS=Rattus norvegicus OX=10116 GN=Chek1 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 7.6e-13
Identity = 46/100 (46.00%), Postives = 59/100 (59.00%), Query Frame = 0


Query: 81  NVVQCYGHEITNTVSGNYIYNLLLEYCSGGSLYDRITKFGPNGLSELEVRRYTRDIVYGI 140
           NVV+ YGH       GN  Y L LEYCSGG L+DRI      G+ E + +R+   ++ G+
Sbjct: 66  NVVKFYGHR----REGNIQY-LFLEYCSGGELFDRIEP--DIGMPEQDAQRFFHQLMAGV 125

Query: 141 YSIHGLGYIHCDIKPENILLIPSFDHGGFLVAKIADFGLA 181
             +HG+G  H DIKPEN+LL    D    L  KI+DFGLA
Sbjct: 126 VYLHGIGITHRDIKPENLLL----DERDNL--KISDFGLA 152

[Close]

BLAST of Bhi03G001900 vs. ExPASy Swiss-Prot
Match: O14757 (Serine/threonine-protein kinase Chk1 OS=Homo sapiens OX=9606 GN=CHEK1 PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 1.0e-12
Identity = 46/100 (46.00%), Postives = 59/100 (59.00%), Query Frame = 0


Query: 81  NVVQCYGHEITNTVSGNYIYNLLLEYCSGGSLYDRITKFGPNGLSELEVRRYTRDIVYGI 140
           NVV+ YGH       GN  Y L LEYCSGG L+DRI      G+ E + +R+   ++ G+
Sbjct: 66  NVVKFYGHR----REGNIQY-LFLEYCSGGELFDRIEP--DIGMPEPDAQRFFHQLMAGV 125

Query: 141 YSIHGLGYIHCDIKPENILLIPSFDHGGFLVAKIADFGLA 181
             +HG+G  H DIKPEN+LL    D    L  KI+DFGLA
Sbjct: 126 VYLHGIGITHRDIKPENLLL----DERDNL--KISDFGLA 152

[Close]

BLAST of Bhi03G001900 vs. NCBI nr
Match: XP_038882350.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 414.1 bits (1063), Expect = 6.9e-112
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0


Query: 1   MKNPFINSSLSPDCDQSLRPKRMITKFPNPNNSNGVQSQTQIDGFDNSHGSFWFRGRLLG 60
           MKNPFINSSLSPDCDQSLRPKRMITKFPNPNNSNGVQSQTQIDGFDNSHGSFWFRGRLLG
Sbjct: 1   MKNPFINSSLSPDCDQSLRPKRMITKFPNPNNSNGVQSQTQIDGFDNSHGSFWFRGRLLG 60

Query: 61  KDSSSLRFEKKVLDAFKSCSNVVQCYGHEITNTVSGNYIYNLLLEYCSGGSLYDRITKFG 120
           KDSSSLRFEKKVLDAFKSCSNVVQCYGHEITNTVSGNYIYNLLLEYCSGGSLYDRITKFG
Sbjct: 61  KDSSSLRFEKKVLDAFKSCSNVVQCYGHEITNTVSGNYIYNLLLEYCSGGSLYDRITKFG 120

Query: 121 PNGLSELEVRRYTRDIVYGIYSIHGLGYIHCDIKPENILLIPSFDHGGFLVAKIADFGLA 180
           PNGLSELEVRRYTRDIVYGIYSIHGLGYIHCDIKPENILLIPSFDHGGFLVAKIADFGLA
Sbjct: 121 PNGLSELEVRRYTRDIVYGIYSIHGLGYIHCDIKPENILLIPSFDHGGFLVAKIADFGLA 180

Query: 181 MQMTNVVNIDDDNSTRGT 199
           MQMTNVVNIDDDNSTRGT
Sbjct: 181 MQMTNVVNIDDDNSTRGT 198

[Close]

BLAST of Bhi03G001900 vs. NCBI nr
Match: XP_023516554.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 151.0 bits (380), Expect = 1.1e-32
Identity = 82/153 (53.59%), Postives = 103/153 (67.32%), Query Frame = 0


Query: 47  NSHGSFWFRGRLLGKDSSSLRFEKKVLDAFKSCSNVVQCYGHEITNTVSGNYIYNLLLEY 106
           NS+  F  +  L+ + SSSL++EK+VL     CSN+V+CYG EIT   SG  IYN++ EY
Sbjct: 91  NSNPEFLVKSCLM-ESSSSLQYEKEVLRNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEY 150

Query: 107 CSGGSLYDRITKFGPNGLSELEVRRYTRDIVYGIYSIH-GLGYIHCDIKPENILLIPSFD 166
           C G SL D I KFG NGL E  VR+YTRDIV+G+  +H   GYIH DIKP NILL PS  
Sbjct: 151 CRGSSLRDHIIKFGRNGLPEPIVRQYTRDIVHGLCYMHCNAGYIHGDIKPRNILLSPSDH 210

Query: 167 HGGFLVAKIADFGLAMQMTNVVNIDDDNSTRGT 199
            GG LVAK+A FGLA ++T  V  D++ S  G+
Sbjct: 211 QGGCLVAKLASFGLARKLTPEVLCDEEISGSGS 242

[Close]

BLAST of Bhi03G001900 vs. NCBI nr
Match: KAG6589837.1 (Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 150.6 bits (379), Expect = 1.4e-32
Identity = 83/153 (54.25%), Postives = 102/153 (66.67%), Query Frame = 0


Query: 47  NSHGSFWFRGRLLGKDSSSLRFEKKVLDAFKSCSNVVQCYGHEITNTVSGNYIYNLLLEY 106
           NS+  F  +  L+ + SSSL++EK+VL     CSN+V+CYG EIT   SG  IYN++ EY
Sbjct: 91  NSNSEFLVKSCLM-ESSSSLQYEKEVLCNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEY 150

Query: 107 CSGGSLYDRITKFGPNGLSELEVRRYTRDIVYGIYSIH-GLGYIHCDIKPENILLIPSFD 166
           C G SL D I KFG NGL E  VR+YTRDIV G+  IH   GYIH DIKP NILL PS  
Sbjct: 151 CCGSSLRDHIIKFGRNGLPEPIVRKYTRDIVRGLCYIHCNAGYIHGDIKPRNILLSPSDR 210

Query: 167 HGGFLVAKIADFGLAMQMTNVVNIDDDNSTRGT 199
            GG LVAK+A FGLA ++T  V  D++ S  G+
Sbjct: 211 KGGCLVAKLASFGLARKLTPEVLCDEEISGSGS 242

[Close]

BLAST of Bhi03G001900 vs. NCBI nr
Match: XP_022960664.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata])

HSP 1 Score: 150.2 bits (378), Expect = 1.9e-32
Identity = 82/153 (53.59%), Postives = 103/153 (67.32%), Query Frame = 0


Query: 47  NSHGSFWFRGRLLGKDSSSLRFEKKVLDAFKSCSNVVQCYGHEITNTVSGNYIYNLLLEY 106
           NS+  F  +  L+ + SSSL++EK+VL     CSN+V+CYG EIT + SG  IYN++ EY
Sbjct: 91  NSNSEFLVKSCLM-ESSSSLQYEKEVLCNLGPCSNLVRCYGDEITMSKSGEQIYNVVFEY 150

Query: 107 CSGGSLYDRITKFGPNGLSELEVRRYTRDIVYGIYSIH-GLGYIHCDIKPENILLIPSFD 166
           C G SL D I KFG NGL E  VR+YTRDIV G+  +H   GYIH DIKP NILL PS  
Sbjct: 151 CCGSSLRDHIIKFGRNGLPEPIVRKYTRDIVRGLCYMHCNAGYIHGDIKPRNILLSPSDH 210

Query: 167 HGGFLVAKIADFGLAMQMTNVVNIDDDNSTRGT 199
            GG LVAK+A FGLA ++T  V  D++ S  G+
Sbjct: 211 KGGCLVAKLASFGLARKLTPEVLCDEEISGSGS 242

[Close]

BLAST of Bhi03G001900 vs. NCBI nr
Match: KAG7023508.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 149.8 bits (377), Expect = 2.4e-32
Identity = 82/153 (53.59%), Postives = 102/153 (66.67%), Query Frame = 0


Query: 47  NSHGSFWFRGRLLGKDSSSLRFEKKVLDAFKSCSNVVQCYGHEITNTVSGNYIYNLLLEY 106
           NS+  F  +  L+ + SSSL++EK+VL     CSN+V+CYG EIT   SG  IYN++ EY
Sbjct: 91  NSNSEFLVKSCLM-ESSSSLQYEKEVLCNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEY 150

Query: 107 CSGGSLYDRITKFGPNGLSELEVRRYTRDIVYGIYSIH-GLGYIHCDIKPENILLIPSFD 166
           C G SL D I KFG NGL E  VR+YTRDIV G+  +H   GYIH DIKP NILL PS  
Sbjct: 151 CCGSSLRDHIIKFGRNGLPEPIVRKYTRDIVRGLCYMHCNAGYIHGDIKPRNILLSPSDH 210

Query: 167 HGGFLVAKIADFGLAMQMTNVVNIDDDNSTRGT 199
            GG LVAK+A FGLA ++T  V  D++ S  G+
Sbjct: 211 KGGCLVAKLASFGLARKLTPEVLCDEEISGSGS 242

[Close]

BLAST of Bhi03G001900 vs. ExPASy TrEMBL
Match: A0A6J1HBP5 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=3662 GN=LOC111461386 PE=4 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 9.0e-33
Identity = 82/153 (53.59%), Postives = 103/153 (67.32%), Query Frame = 0


Query: 47  NSHGSFWFRGRLLGKDSSSLRFEKKVLDAFKSCSNVVQCYGHEITNTVSGNYIYNLLLEY 106
           NS+  F  +  L+ + SSSL++EK+VL     CSN+V+CYG EIT + SG  IYN++ EY
Sbjct: 91  NSNSEFLVKSCLM-ESSSSLQYEKEVLCNLGPCSNLVRCYGDEITMSKSGEQIYNVVFEY 150

Query: 107 CSGGSLYDRITKFGPNGLSELEVRRYTRDIVYGIYSIH-GLGYIHCDIKPENILLIPSFD 166
           C G SL D I KFG NGL E  VR+YTRDIV G+  +H   GYIH DIKP NILL PS  
Sbjct: 151 CCGSSLRDHIIKFGRNGLPEPIVRKYTRDIVRGLCYMHCNAGYIHGDIKPRNILLSPSDH 210

Query: 167 HGGFLVAKIADFGLAMQMTNVVNIDDDNSTRGT 199
            GG LVAK+A FGLA ++T  V  D++ S  G+
Sbjct: 211 KGGCLVAKLASFGLARKLTPEVLCDEEISGSGS 242

[Close]

BLAST of Bhi03G001900 vs. ExPASy TrEMBL
Match: A0A6J1JAH8 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=3661 GN=LOC111485031 PE=4 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 1.3e-31
Identity = 77/139 (55.40%), Postives = 95/139 (68.35%), Query Frame = 0


Query: 61  KDSSSLRFEKKVLDAFKSCSNVVQCYGHEITNTVSGNYIYNLLLEYCSGGSLYDRITKFG 120
           + SSSL++EK+VL     CSN+V+CYG EIT   SG  IYN++ EYC G SL D I KFG
Sbjct: 2   ESSSSLQYEKEVLCNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKFG 61

Query: 121 PNGLSELEVRRYTRDIVYGIYSIH-GLGYIHCDIKPENILLIPSFDHGGFLVAKIADFGL 180
            NGL E  VR+YTRDIV+G+  +H    YIH DIKP NILL PS   GG LVAK+A FGL
Sbjct: 62  RNGLPEPIVRKYTRDIVHGLCYMHCNARYIHGDIKPRNILLSPSDHKGGCLVAKLASFGL 121

Query: 181 AMQMTNVVNIDDDNSTRGT 199
           A ++T  V  D++ S  G+
Sbjct: 122 ARKLTPEVLCDEEISGSGS 140

[Close]

BLAST of Bhi03G001900 vs. ExPASy TrEMBL
Match: A0A6S7MWQ1 (Protein kinase domain-containing protein OS=Lactuca saligna OX=75948 GN=LSAL_LOCUS20434 PE=3 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 4.2e-30
Identity = 66/118 (55.93%), Postives = 88/118 (74.58%), Query Frame = 0


Query: 63  SSSLRFEKKVLDAFKSCSNVVQCYGHEITNTVSGNYIYNLLLEYCSGGSLYDRITKFGPN 122
           SSS++ E++VL+  + C NV++C+G EIT   +G  +YNLLLEY SGG+L D I K GPN
Sbjct: 91  SSSIQNEREVLNNLRGCRNVIRCFGEEITTGENGQMVYNLLLEYGSGGTLADLIKKSGPN 150

Query: 123 GLSELEVRRYTRDIVYGIYSIHGLGYIHCDIKPENILLIPSFDHGGFLVAKIADFGLA 181
           GL E +V+R+TR I++G+  IH  GY+HCD+KPENILL+ S  +GGF  AKI D GLA
Sbjct: 151 GLLESDVKRHTRSILHGLRHIHHNGYVHCDLKPENILLVGSSSNGGF-TAKIGDLGLA 207

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BLAST of Bhi03G001900 vs. ExPASy TrEMBL
Match: A0A6S7MW80 (Protein kinase domain-containing protein OS=Lactuca saligna OX=75948 GN=LSAL_LOCUS20431 PE=3 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 5.5e-30
Identity = 65/118 (55.08%), Postives = 89/118 (75.42%), Query Frame = 0


Query: 63  SSSLRFEKKVLDAFKSCSNVVQCYGHEITNTVSGNYIYNLLLEYCSGGSLYDRITKFGPN 122
           SSS++ E++V++  + C NV++C+G EIT   +G  +YNLLLEY SGG+L D I K GPN
Sbjct: 91  SSSIQNEREVVNNLRGCRNVIRCFGEEITTGENGQMVYNLLLEYGSGGTLADLIKKSGPN 150

Query: 123 GLSELEVRRYTRDIVYGIYSIHGLGYIHCDIKPENILLIPSFDHGGFLVAKIADFGLA 181
           GL E +V+R+TR I++G+  IH  GY+HCD+KPENILL+ S  +GGF+ AKI D GLA
Sbjct: 151 GLLESDVKRHTRSILHGLRHIHHNGYVHCDLKPENILLVGSSSNGGFM-AKIGDLGLA 207

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BLAST of Bhi03G001900 vs. ExPASy TrEMBL
Match: A0A6A1VNJ4 (Mitogen-activated protein kinase kinase kinase ANP1 OS=Morella rubra OX=262757 GN=CJ030_MR5G003188 PE=3 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 9.3e-30
Identity = 72/166 (43.37%), Postives = 94/166 (56.63%), Query Frame = 0


Query: 48  SHGSFWFRGRLLGKD---------------------------------SSSLRFEKKVLD 107
           SHG  W RG ++GK                                  S SL+ EK+VLD
Sbjct: 12  SHGESWMRGPMIGKGSFGSVFLATSKKPRSRFDCFPAAMAVKSAEVSVSGSLQKEKEVLD 71

Query: 108 AFKSCSNVVQCYGHEITNTVSGNYIYNLLLEYCSGGSLYDRITKFGPNGLSELEVRRYTR 167
             K C  V+ C+G EIT   +G  ++NLLLEY SGG+L + I K   +GL E +V+RYTR
Sbjct: 72  NVKGCPFVIGCFGEEITTQENGEMVFNLLLEYASGGTLAESIEKSSGSGLPESDVKRYTR 131

Query: 168 DIVYGIYSIHGLGYIHCDIKPENILLIPSFDHGGFLVAKIADFGLA 181
            I+ G+  IH  GY+HCD+KPEN+LL+P+ +  G LVAKI DFGLA
Sbjct: 132 SILKGLGHIHDCGYVHCDLKPENVLLVPAVNGSGNLVAKIGDFGLA 177

The following BLAST results are available for this feature:

BLAST of Bhi03G001900 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G36950.1	5.2e-25	48.44	mitogen-activated protein kinase kinase kinase 21	[more]
AT5G27510.1	3.4e-24	37.66	Protein kinase superfamily protein	[more]
AT2G05060.1	4.4e-24	44.17	Protein kinase superfamily protein	[more]
AT3G50310.1	1.4e-22	46.28	mitogen-activated protein kinase kinase kinase 20	[more]
AT3G45790.1	1.9e-22	48.82	Protein kinase superfamily protein	[more]

BLAST of Bhi03G001900 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SND6	2.0e-21	46.28	Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370...	[more]
O80888	1.5e-13	32.50	Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370...	[more]
O35280	7.6e-13	43.69	Serine/threonine-protein kinase Chk1 OS=Mus musculus OX=10090 GN=Chek1 PE=1 SV=2	[more]
Q91ZN7	7.6e-13	46.00	Serine/threonine-protein kinase Chk1 OS=Rattus norvegicus OX=10116 GN=Chek1 PE=2...	[more]
O14757	1.0e-12	46.00	Serine/threonine-protein kinase Chk1 OS=Homo sapiens OX=9606 GN=CHEK1 PE=1 SV=2	[more]

BLAST of Bhi03G001900 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038882350.1	6.9e-112	100.00	mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida]	[more]
XP_023516554.1	1.1e-32	53.59	mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pe...	[more]
KAG6589837.1	1.4e-32	54.25	Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosper...	[more]
XP_022960664.1	1.9e-32	53.59	mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata]	[more]
KAG7023508.1	2.4e-32	53.59	Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper...	[more]

BLAST of Bhi03G001900 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HBP5	9.0e-33	53.59	mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=...	[more]
A0A6J1JAH8	1.3e-31	55.40	mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=36...	[more]
A0A6S7MWQ1	4.2e-30	55.93	Protein kinase domain-containing protein OS=Lactuca saligna OX=75948 GN=LSAL_LOC...	[more]
A0A6S7MW80	5.5e-30	55.08	Protein kinase domain-containing protein OS=Lactuca saligna OX=75948 GN=LSAL_LOC...	[more]
A0A6A1VNJ4	9.3e-30	43.37	Mitogen-activated protein kinase kinase kinase ANP1 OS=Morella rubra OX=262757 G...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 53..195 e-value: 1.5E-5 score: -56.7
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 64..189 e-value: 1.2E-19 score: 70.7
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 1..198 score: 20.400322
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 57..198 e-value: 6.3E-27 score: 96.6
None	No IPR available	PANTHER	PTHR48011:SF56	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 3-LIKE	coord: 62..198
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 62..198
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 148..160
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 64..186

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi03M001900	Bhi03M001900	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity