Bhi03G001808 (gene) Wax gourd (B227) v1

Overview
NameBhi03G001808
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr3: 50123256 .. 50125527 (+)
RNA-Seq ExpressionBhi03G001808
SyntenyBhi03G001808
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGAATTAGATCAAGAAGTAATTGACCCTCCTCCCTTCTTTCTCTGTCCCATCTCTTTGCAAATCATGAAAGACCCCGTCACGATCGCCTCCGGCATAACCTACGATCGTGAAAACATCGAGAAATGGTTGTTTTCTGACAAACATAAGACATGTCCTGTCTCGAAGGTCGTTTTGTTAGACTTCGATATCACTCCCAACCATACTTTAAGACGAGTCATTCAAGCTTGGTGCACCGCCAATGCTACAAGCGGTGTTGAGAGGATACCGACGCCGAAGCCCCCAGTCGACCGAAAGCAAGTCGTTCGGATTCTCGCTGATGCTAAGTTATCTCCATTCTCTCAACAGAATTGTCTTCGTCGTCTTCGATCTATTGCTGCGGCGAATGAAACTAATAAACGTTGCATGGAAGCTGCTGGAGTTGTGGAGTTCTTGGCTGGGCTTGTTTGTAGTTCTACAACCAACGTGGAGTCTGGCTTGGAAGGTACTATACAAAACTCTCCTCTAGTTTTTTTTTCCCCTTTTTTATTTACTTTTTAGGAGCGTTTTCAAGTTAAATTTGAAAACTATTTTTTTAAAAAAATTACTTTTTATTTTTAAGAGCTTATTTATATTCAATTTTTTATTTTTAGTTTTCGAAAATTAAATTTATAAATACTAGTTATCTATTTTTAATATGCCAAGATTAATCTAAGAAAATTTTAAAAAAAATTAATTTTTAGAATTCGATAAGAATTTGAATTTATTTATTTATTTAAGAGGAATAAAAGTATAGTTAAGAAATTGCAGAAAAAACCCTAATTTTAAAGAAAAAAAAACATAAATTAAATTATTGTCGAGCCTAAAATATGTGCTTTTATTTTCAGAATTTAAGTAAGAATTAGTGAAATATTCCTTAATAAAGATTAAAACAGTTTTAAACAAAATTGTGAAACAACTAACCTAAAATTTAAAATTTTAAAATCTAATTTATAACTTTTAGTTTTAAACTTTAAAAAGTGAATTGAAAGTTTCTTAAACCTATTTTGTTAATAAATTTGTCAACTTTAAAAGGTGTCTAATATATTATTAAACTTTCAATGTCGAAAAATAGTTTGATTATAATTAATTTATTTTTTAAAAAAATTAATTAATAAGAATTTAATACTCATTTAAAGTCTAAGAATCAAAGTTAAAAGTGTAAATCTAACTTCGAATTTTAAGAACCAAATGAAAATAAAATTCAAAACTTAGATATGAAGGATAAAAGTATAACATTTTAAAATTTAGAGATTAAATAAAAATTTAGCTCAAAATCTAGTGATAAAATGTAATATTATTTCCTTTTTAACTTTTAAGCATAGTTTTTTTCTTTTTTTCTTTTTTTCAATAATAATAATAAAGCAAAAGTTTGAACTTTAAACATTATCCCTTCATTATAAATGTGTAATATAATGTATAATATAAGTTGATAATTTAATGTTGGTTATGTAATGACGCAGATTACGCGTTTGACGACATGTCGGCAAATTTAGGGGATGAAGCTTTGAGCATTCTTTACAAACTCCAAATCTCCGAATCCTCTCTGAAATTTCTCCTCTCCAACAATGGCGGCATCTTCATCTCCACCTTAACCAAAATCTTACAAAATCGAAGCTACTCCTCTCGAGCATATTCAATAATGCTTCTCAATTCAATGTTCGAAGTTGCAGATCAGATTCAAATCCTCAATCTCACCACCGATTTCTTCATCGAAATCGTCCAAATACTTAAAGATCAGATCTCCAAACAAGCCTCGAAATCGGCGTTGAAATTGCTAATCGGTGTCTGTTCGAGTATCAGAAACAGAGTAAAGGCGGTTGAAGCCGGCGCGGTTCCGGTTCTGGTCGATCTCCTCCTCGATCTTGATTCATCGGAGAAGCGATTGAACGAGATGATTCTTGTGGTGCTTGATCTGCTCTGTGGCTGCGCTGATGGCAGAGCGGGGCTGTTGAATCACGCGGCGGGGATCGCCGTCGTGTCGAAGAAGATTCTGAGAGTTTCGTCGCTTGGAAGTGAGAAGGCGGTTGGGATATTGTGCTCCGTTGCGAGATTCTCGGCGAGCCAAAGCGTTCTCCAGGAAATGGTGCGGCTCGGAGTTGTGACGAAGCTTTGTTTTCTGCTTCAAGTGGTCGGCGCCGGAGTGAAAGCGAAAGAGAAAGCTAAAGAGATTCTGAAGCTTCATGGTCGGACATGGCGGAATTCTTCTTGTTTGCCTTCGAGTCTGCGATCAGCTTATCCACAACATTAG

mRNA sequence

ATGGCAGAATTAGATCAAGAAGTAATTGACCCTCCTCCCTTCTTTCTCTGTCCCATCTCTTTGCAAATCATGAAAGACCCCGTCACGATCGCCTCCGGCATAACCTACGATCGTGAAAACATCGAGAAATGGTTGTTTTCTGACAAACATAAGACATGTCCTGTCTCGAAGGTCGTTTTGTTAGACTTCGATATCACTCCCAACCATACTTTAAGACGAGTCATTCAAGCTTGGTGCACCGCCAATGCTACAAGCGGTGTTGAGAGGATACCGACGCCGAAGCCCCCAGTCGACCGAAAGCAAGTCGTTCGGATTCTCGCTGATGCTAAGTTATCTCCATTCTCTCAACAGAATTGTCTTCGTCGTCTTCGATCTATTGCTGCGGCGAATGAAACTAATAAACGTTGCATGGAAGCTGCTGGAGTTGTGGAGTTCTTGGCTGGGCTTGTTTGTAGTTCTACAACCAACGTGGAGTCTGGCTTGGAAGATTACGCGTTTGACGACATGTCGGCAAATTTAGGGGATGAAGCTTTGAGCATTCTTTACAAACTCCAAATCTCCGAATCCTCTCTGAAATTTCTCCTCTCCAACAATGGCGGCATCTTCATCTCCACCTTAACCAAAATCTTACAAAATCGAAGCTACTCCTCTCGAGCATATTCAATAATGCTTCTCAATTCAATGTTCGAAGTTGCAGATCAGATTCAAATCCTCAATCTCACCACCGATTTCTTCATCGAAATCGTCCAAATACTTAAAGATCAGATCTCCAAACAAGCCTCGAAATCGGCGTTGAAATTGCTAATCGGTGTCTGTTCGAGTATCAGAAACAGAGTAAAGGCGGTTGAAGCCGGCGCGGTTCCGGTTCTGGTCGATCTCCTCCTCGATCTTGATTCATCGGAGAAGCGATTGAACGAGATGATTCTTGTGGTGCTTGATCTGCTCTGTGGCTGCGCTGATGGCAGAGCGGGGCTGTTGAATCACGCGGCGGGGATCGCCGTCGTGTCGAAGAAGATTCTGAGAGTTTCGTCGCTTGGAAGTGAGAAGGCGGTTGGGATATTGTGCTCCGTTGCGAGATTCTCGGCGAGCCAAAGCGTTCTCCAGGAAATGGTGCGGCTCGGAGTTGTGACGAAGCTTTGTTTTCTGCTTCAAGTGGTCGGCGCCGGAGTGAAAGCGAAAGAGAAAGCTAAAGAGATTCTGAAGCTTCATGGTCGGACATGGCGGAATTCTTCTTGTTTGCCTTCGAGTCTGCGATCAGCTTATCCACAACATTAG

Coding sequence (CDS)

ATGGCAGAATTAGATCAAGAAGTAATTGACCCTCCTCCCTTCTTTCTCTGTCCCATCTCTTTGCAAATCATGAAAGACCCCGTCACGATCGCCTCCGGCATAACCTACGATCGTGAAAACATCGAGAAATGGTTGTTTTCTGACAAACATAAGACATGTCCTGTCTCGAAGGTCGTTTTGTTAGACTTCGATATCACTCCCAACCATACTTTAAGACGAGTCATTCAAGCTTGGTGCACCGCCAATGCTACAAGCGGTGTTGAGAGGATACCGACGCCGAAGCCCCCAGTCGACCGAAAGCAAGTCGTTCGGATTCTCGCTGATGCTAAGTTATCTCCATTCTCTCAACAGAATTGTCTTCGTCGTCTTCGATCTATTGCTGCGGCGAATGAAACTAATAAACGTTGCATGGAAGCTGCTGGAGTTGTGGAGTTCTTGGCTGGGCTTGTTTGTAGTTCTACAACCAACGTGGAGTCTGGCTTGGAAGATTACGCGTTTGACGACATGTCGGCAAATTTAGGGGATGAAGCTTTGAGCATTCTTTACAAACTCCAAATCTCCGAATCCTCTCTGAAATTTCTCCTCTCCAACAATGGCGGCATCTTCATCTCCACCTTAACCAAAATCTTACAAAATCGAAGCTACTCCTCTCGAGCATATTCAATAATGCTTCTCAATTCAATGTTCGAAGTTGCAGATCAGATTCAAATCCTCAATCTCACCACCGATTTCTTCATCGAAATCGTCCAAATACTTAAAGATCAGATCTCCAAACAAGCCTCGAAATCGGCGTTGAAATTGCTAATCGGTGTCTGTTCGAGTATCAGAAACAGAGTAAAGGCGGTTGAAGCCGGCGCGGTTCCGGTTCTGGTCGATCTCCTCCTCGATCTTGATTCATCGGAGAAGCGATTGAACGAGATGATTCTTGTGGTGCTTGATCTGCTCTGTGGCTGCGCTGATGGCAGAGCGGGGCTGTTGAATCACGCGGCGGGGATCGCCGTCGTGTCGAAGAAGATTCTGAGAGTTTCGTCGCTTGGAAGTGAGAAGGCGGTTGGGATATTGTGCTCCGTTGCGAGATTCTCGGCGAGCCAAAGCGTTCTCCAGGAAATGGTGCGGCTCGGAGTTGTGACGAAGCTTTGTTTTCTGCTTCAAGTGGTCGGCGCCGGAGTGAAAGCGAAAGAGAAAGCTAAAGAGATTCTGAAGCTTCATGGTCGGACATGGCGGAATTCTTCTTGTTTGCCTTCGAGTCTGCGATCAGCTTATCCACAACATTAG

Protein sequence

MAELDQEVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVLLDFDITPNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAKLSPFSQQNCLRRLRSIAAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAFDDMSANLGDEALSILYKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQILNLTTDFFIEIVQILKDQISKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVDLLLDLDSSEKRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSVARFSASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSSLRSAYPQH
Homology
BLAST of Bhi03G001808 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 432.2 bits (1110), Expect = 4.9e-121
Identity = 235/436 (53.90%), Postives = 312/436 (71.56%), Query Frame = 0

Query: 4   LDQEVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVLLDF 63
           +DQE I+ P FFLCPISL IMKDPV +++GITYDRE+IEKWLFS K  +CPV+K V+ + 
Sbjct: 1   MDQE-IEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITET 60

Query: 64  DITPNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAKLSPFSQQNCLRRL 123
           D+TPNHTLRR+IQ+WCT NA+ G+ERIPTPKPP+ + ++ +++ ++  S  +Q  CL+RL
Sbjct: 61  DLTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRL 120

Query: 124 RSIAAANETNKRCMEAAGVVEFLAGLVCSST---TNVESGLEDYAFDDM----------- 183
           R I + N TNKRC+EAA V EFLA +V +S     +  S L     +DM           
Sbjct: 121 RQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFD 180

Query: 184 -SANLGDEALSILYKLQISESSLKFLLSNNGGI-FISTLTKILQNRSYSSRAYSIMLLNS 243
            S +L DEALS+LY L  SE++LK LL+N  G   + TLTKI+Q   Y SRAY+ +LL  
Sbjct: 181 SSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKK 240

Query: 244 MFEVADQIQILNLTTDFFIEIVQILKDQISKQASKSALKLLIGVCSSIRNRVKAVEAGAV 303
           + EVAD +QI+ L  + F E++QIL DQIS +A++SA+++L+  C   RNR KAVE G +
Sbjct: 241 LLEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTI 300

Query: 304 PVLVDLLL-DLDSSEKRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLG 363
            ++++LL+ D  SSE+R +EM +VVLD+LC CA+GRA  LNH A IAVVSKKILRVS + 
Sbjct: 301 SMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQIT 360

Query: 364 SEKAVGILCSVARFSASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRT 423
           SE+AV +L SV RF A+ S+LQEM++LGVV KLC +LQ V  G K KEKAKE+LKLH R 
Sbjct: 361 SERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARV 420

BLAST of Bhi03G001808 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 421.0 bits (1081), Expect = 1.1e-117
Identity = 222/418 (53.11%), Postives = 302/418 (72.25%), Query Frame = 0

Query: 7   EVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVLLDFDIT 66
           E I+ PPFFLCPISL+IMKDPV +++GITYDR++IEKWLF+ K  +CPV+K  + D D+T
Sbjct: 8   EEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLT 67

Query: 67  PNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAKLSPFSQQNCLRRLRSI 126
           PNHTLRR+IQ+WCT NA+ GVERIPTP+PP+ + ++ +++ D+  S  +Q  CL+RLR I
Sbjct: 68  PNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQI 127

Query: 127 AAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAFDDMSANLGDEALSILYKLQI 186
            + N TNKRC+EAAGV EFLA +V + + N               +L DEAL++LY L+ 
Sbjct: 128 VSENATNKRCLEAAGVPEFLANIVSNDSEN--------------GSLTDEALNLLYHLET 187

Query: 187 SESSLKFLLSN-NGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQILNLTTDFF 246
           SE+ LK LL+N      + +LTKI+Q   Y SR Y+ +LL ++ EVAD +Q + L  + F
Sbjct: 188 SETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVF 247

Query: 247 IEIVQILKDQISKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVDLLLDLD-SSEKRL 306
            E+VQIL D+IS++A+K+A+ +L+ +C   RNR KAVEAG + V+++LL+D   +SE+R 
Sbjct: 248 TEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRG 307

Query: 307 NEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSVARFSASQ 366
            EM +VVLDLLC CA+GRA  LNH A IAVV KKILRVS   S++AV +L SV RF A+ 
Sbjct: 308 PEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATP 367

Query: 367 SVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSSLRSAYP 423
           ++L EM++LGVV KLC +LQ V  G K KEKAKE+LKLH R W++S CLP ++  AYP
Sbjct: 368 ALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of Bhi03G001808 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 247.3 bits (630), Expect = 2.2e-65
Identity = 169/452 (37.39%), Postives = 246/452 (54.42%), Query Frame = 0

Query: 5   DQEVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVL-LDF 64
           ++E I+ P +F+CPISL+IMKDPVT  SGITYDR+NI KWL  +K  +CPV+K  L LD 
Sbjct: 18  EEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDS 77

Query: 65  DITPNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAK---LSPFSQQNCL 124
           D+TPNH LRR+IQ WC  N T GV RI TP+ P  +  VV  + + K        ++  L
Sbjct: 78  DLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETL 137

Query: 125 RRLRSIAAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAFDDMSANLGDEALSI 184
           ++L  + A +  N+R M   GV + L   V   T+  E G              DE+L +
Sbjct: 138 QKL-EVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRR-------RIKGLDESLRL 197

Query: 185 LYKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQILNL 244
           L+ + I  +  K +L  N  + + +LT +L    + S+AY+I+LL ++ E      +  L
Sbjct: 198 LHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERL 257

Query: 245 TTDFFIEIVQILKDQIS-----------------------------------KQASKSAL 304
             + F  I+  LKD ++                                   KQA  +AL
Sbjct: 258 NPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAAL 317

Query: 305 KLLIGVCSSIRNRVKAVEAGAVPVLVDLLLDLDSSEKRLNEMILVVLDLLCGCADGRAGL 364
            +L+   S  RNR   V+ GAV  L++L +   + EKR+ E++L VL  LC CA+GRA +
Sbjct: 318 MILLETSSWSRNRSLLVDLGAVSELIELEISY-TGEKRITELMLGVLSRLCCCANGRAEI 377

Query: 365 LNHAAGIAVVSKKILRVSSLGSEKAVGILCSVARFSASQSVLQEMVRLGVVTKLCFLLQV 418
           L H  GIAVV+K++LRVS    ++A+ IL +V++FS    V++EMV +G V KLC +L  
Sbjct: 378 LAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLG- 437

BLAST of Bhi03G001808 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 195.3 bits (495), Expect = 1.0e-49
Identity = 145/419 (34.61%), Postives = 227/419 (54.18%), Query Frame = 0

Query: 9   IDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVLLDFDITPN 68
           I  P  F CPISL++M+DPVT+ +G TYDR +IE W+    + TCPV++  L DF + PN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 69  HTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAK------LSPFSQQNCLRR 128
           HTLRR+IQ WC AN ++GVERIPTPK P D   V  +L+ A       +S  S+   LRR
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRR 131

Query: 129 LRSIAAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAFDDMSANLGDEALSILY 188
           LR  A  ++ N+  + A    E L  ++ S TT              S+ L  E+L++L 
Sbjct: 132 LRGFARDSDKNRVLIAAHNATEILIKILFSETT--------------SSELVSESLALLV 191

Query: 189 KLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSR---AYSIMLLNSMFEVADQIQILN 248
            L I+E +    +S++ G  +  LT++L + S  +R   A  I ++++  + AD    ++
Sbjct: 192 MLPITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSIS 251

Query: 249 LTTDFFIEIVQILKDQI-SKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVD-LLLDL 308
            +   F  ++ +L++ I S++A K  +K L  +CS    R  A+ AGA  +L+D L  D 
Sbjct: 252 NSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADF 311

Query: 309 DSSEKRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSV 368
           D  +    E  L  ++LLC   +G A    HA  + ++ K ILRVS   +E A G L  +
Sbjct: 312 DRCD---TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGAL--L 371

Query: 369 ARFSASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSS 417
           A  +A +   +E    GVV +L  ++Q      +AK+KA+++LKL   +W + +   +S
Sbjct: 372 ALCTAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSWPDYNSFANS 409

BLAST of Bhi03G001808 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 186.8 bits (473), Expect = 3.6e-47
Identity = 140/418 (33.49%), Postives = 221/418 (52.87%), Query Frame = 0

Query: 9   IDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVLLDFDITPN 68
           I  P  F CPISL +M DPVTI++G TYDR +I+ W+ +  + TCPV++V L DF + PN
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSDFTLIPN 71

Query: 69  HTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAK------LSPFSQQNCLRR 128
           HTLRR+IQ WC AN ++GVERIPTPK P D   V  +L+ A       +S  S+   +RR
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRR 131

Query: 129 LRSIAAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAFDDMSANLGDEALSILY 188
           LR +A  +E N+  +      E L  ++            D     +S+ L  E+L++L 
Sbjct: 132 LRGLARDSEKNRVLIAGHNAREILVRIL----------FADIETTSLSSELVSESLALLV 191

Query: 189 KLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQ---ILN 248
            L ++E+  + + S+     +  +T++L + S   R  +  L+  +   A  +    I++
Sbjct: 192 LLHMTETECEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIIS 251

Query: 249 LTTDFFIEIVQILKDQI-SKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVD-LLLDL 308
            +   F  ++ +LK+ I S++A K  +K +  +C   + R  A+ AGA  +L+D L  D 
Sbjct: 252 GSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADF 311

Query: 309 DSSEKRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSV 368
           D  +    E  L  ++LLC   +G A    HA  + ++ K ILRVS   +E A G L  +
Sbjct: 312 DRCD---TERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGAL--L 371

Query: 369 ARFSASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPS 416
           A  +A +    E    G+VT+L  L+Q      +AK KA+ +LKL   +W + S + S
Sbjct: 372 ALCTAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDSTVHS 410

BLAST of Bhi03G001808 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 6.9e-120
Identity = 235/436 (53.90%), Postives = 312/436 (71.56%), Query Frame = 0

Query: 4   LDQEVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVLLDF 63
           +DQE I+ P FFLCPISL IMKDPV +++GITYDRE+IEKWLFS K  +CPV+K V+ + 
Sbjct: 1   MDQE-IEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITET 60

Query: 64  DITPNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAKLSPFSQQNCLRRL 123
           D+TPNHTLRR+IQ+WCT NA+ G+ERIPTPKPP+ + ++ +++ ++  S  +Q  CL+RL
Sbjct: 61  DLTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRL 120

Query: 124 RSIAAANETNKRCMEAAGVVEFLAGLVCSST---TNVESGLEDYAFDDM----------- 183
           R I + N TNKRC+EAA V EFLA +V +S     +  S L     +DM           
Sbjct: 121 RQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFD 180

Query: 184 -SANLGDEALSILYKLQISESSLKFLLSNNGGI-FISTLTKILQNRSYSSRAYSIMLLNS 243
            S +L DEALS+LY L  SE++LK LL+N  G   + TLTKI+Q   Y SRAY+ +LL  
Sbjct: 181 SSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKK 240

Query: 244 MFEVADQIQILNLTTDFFIEIVQILKDQISKQASKSALKLLIGVCSSIRNRVKAVEAGAV 303
           + EVAD +QI+ L  + F E++QIL DQIS +A++SA+++L+  C   RNR KAVE G +
Sbjct: 241 LLEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTI 300

Query: 304 PVLVDLLL-DLDSSEKRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLG 363
            ++++LL+ D  SSE+R +EM +VVLD+LC CA+GRA  LNH A IAVVSKKILRVS + 
Sbjct: 301 SMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQIT 360

Query: 364 SEKAVGILCSVARFSASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRT 423
           SE+AV +L SV RF A+ S+LQEM++LGVV KLC +LQ V  G K KEKAKE+LKLH R 
Sbjct: 361 SERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARV 420

BLAST of Bhi03G001808 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 1.6e-116
Identity = 222/418 (53.11%), Postives = 302/418 (72.25%), Query Frame = 0

Query: 7   EVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVLLDFDIT 66
           E I+ PPFFLCPISL+IMKDPV +++GITYDR++IEKWLF+ K  +CPV+K  + D D+T
Sbjct: 8   EEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLT 67

Query: 67  PNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAKLSPFSQQNCLRRLRSI 126
           PNHTLRR+IQ+WCT NA+ GVERIPTP+PP+ + ++ +++ D+  S  +Q  CL+RLR I
Sbjct: 68  PNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQI 127

Query: 127 AAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAFDDMSANLGDEALSILYKLQI 186
            + N TNKRC+EAAGV EFLA +V + + N               +L DEAL++LY L+ 
Sbjct: 128 VSENATNKRCLEAAGVPEFLANIVSNDSEN--------------GSLTDEALNLLYHLET 187

Query: 187 SESSLKFLLSN-NGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQILNLTTDFF 246
           SE+ LK LL+N      + +LTKI+Q   Y SR Y+ +LL ++ EVAD +Q + L  + F
Sbjct: 188 SETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVF 247

Query: 247 IEIVQILKDQISKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVDLLLDLD-SSEKRL 306
            E+VQIL D+IS++A+K+A+ +L+ +C   RNR KAVEAG + V+++LL+D   +SE+R 
Sbjct: 248 TEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRG 307

Query: 307 NEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSVARFSASQ 366
            EM +VVLDLLC CA+GRA  LNH A IAVV KKILRVS   S++AV +L SV RF A+ 
Sbjct: 308 PEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATP 367

Query: 367 SVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSSLRSAYP 423
           ++L EM++LGVV KLC +LQ V  G K KEKAKE+LKLH R W++S CLP ++  AYP
Sbjct: 368 ALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of Bhi03G001808 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 3.1e-64
Identity = 169/452 (37.39%), Postives = 246/452 (54.42%), Query Frame = 0

Query: 5   DQEVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVL-LDF 64
           ++E I+ P +F+CPISL+IMKDPVT  SGITYDR+NI KWL  +K  +CPV+K  L LD 
Sbjct: 4   EEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDS 63

Query: 65  DITPNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAK---LSPFSQQNCL 124
           D+TPNH LRR+IQ WC  N T GV RI TP+ P  +  VV  + + K        ++  L
Sbjct: 64  DLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETL 123

Query: 125 RRLRSIAAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAFDDMSANLGDEALSI 184
           ++L  + A +  N+R M   GV + L   V   T+  E G              DE+L +
Sbjct: 124 QKL-EVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRR-------RIKGLDESLRL 183

Query: 185 LYKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQILNL 244
           L+ + I  +  K +L  N  + + +LT +L    + S+AY+I+LL ++ E      +  L
Sbjct: 184 LHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERL 243

Query: 245 TTDFFIEIVQILKDQIS-----------------------------------KQASKSAL 304
             + F  I+  LKD ++                                   KQA  +AL
Sbjct: 244 NPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAAL 303

Query: 305 KLLIGVCSSIRNRVKAVEAGAVPVLVDLLLDLDSSEKRLNEMILVVLDLLCGCADGRAGL 364
            +L+   S  RNR   V+ GAV  L++L +   + EKR+ E++L VL  LC CA+GRA +
Sbjct: 304 MILLETSSWSRNRSLLVDLGAVSELIELEISY-TGEKRITELMLGVLSRLCCCANGRAEI 363

Query: 365 LNHAAGIAVVSKKILRVSSLGSEKAVGILCSVARFSASQSVLQEMVRLGVVTKLCFLLQV 418
           L H  GIAVV+K++LRVS    ++A+ IL +V++FS    V++EMV +G V KLC +L  
Sbjct: 364 LAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLG- 423

BLAST of Bhi03G001808 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.4e-48
Identity = 145/419 (34.61%), Postives = 227/419 (54.18%), Query Frame = 0

Query: 9   IDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVLLDFDITPN 68
           I  P  F CPISL++M+DPVT+ +G TYDR +IE W+    + TCPV++  L DF + PN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 69  HTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAK------LSPFSQQNCLRR 128
           HTLRR+IQ WC AN ++GVERIPTPK P D   V  +L+ A       +S  S+   LRR
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRR 131

Query: 129 LRSIAAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAFDDMSANLGDEALSILY 188
           LR  A  ++ N+  + A    E L  ++ S TT              S+ L  E+L++L 
Sbjct: 132 LRGFARDSDKNRVLIAAHNATEILIKILFSETT--------------SSELVSESLALLV 191

Query: 189 KLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSR---AYSIMLLNSMFEVADQIQILN 248
            L I+E +    +S++ G  +  LT++L + S  +R   A  I ++++  + AD    ++
Sbjct: 192 MLPITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSIS 251

Query: 249 LTTDFFIEIVQILKDQI-SKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVD-LLLDL 308
            +   F  ++ +L++ I S++A K  +K L  +CS    R  A+ AGA  +L+D L  D 
Sbjct: 252 NSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADF 311

Query: 309 DSSEKRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSV 368
           D  +    E  L  ++LLC   +G A    HA  + ++ K ILRVS   +E A G L  +
Sbjct: 312 DRCD---TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGAL--L 371

Query: 369 ARFSASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSS 417
           A  +A +   +E    GVV +L  ++Q      +AK+KA+++LKL   +W + +   +S
Sbjct: 372 ALCTAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSWPDYNSFANS 409

BLAST of Bhi03G001808 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 5.0e-46
Identity = 140/418 (33.49%), Postives = 221/418 (52.87%), Query Frame = 0

Query: 9   IDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVLLDFDITPN 68
           I  P  F CPISL +M DPVTI++G TYDR +I+ W+ +  + TCPV++V L DF + PN
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSDFTLIPN 71

Query: 69  HTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAK------LSPFSQQNCLRR 128
           HTLRR+IQ WC AN ++GVERIPTPK P D   V  +L+ A       +S  S+   +RR
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRR 131

Query: 129 LRSIAAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAFDDMSANLGDEALSILY 188
           LR +A  +E N+  +      E L  ++            D     +S+ L  E+L++L 
Sbjct: 132 LRGLARDSEKNRVLIAGHNAREILVRIL----------FADIETTSLSSELVSESLALLV 191

Query: 189 KLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQ---ILN 248
            L ++E+  + + S+     +  +T++L + S   R  +  L+  +   A  +    I++
Sbjct: 192 LLHMTETECEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIIS 251

Query: 249 LTTDFFIEIVQILKDQI-SKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVD-LLLDL 308
            +   F  ++ +LK+ I S++A K  +K +  +C   + R  A+ AGA  +L+D L  D 
Sbjct: 252 GSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADF 311

Query: 309 DSSEKRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSV 368
           D  +    E  L  ++LLC   +G A    HA  + ++ K ILRVS   +E A G L  +
Sbjct: 312 DRCD---TERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGAL--L 371

Query: 369 ARFSASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPS 416
           A  +A +    E    G+VT+L  L+Q      +AK KA+ +LKL   +W + S + S
Sbjct: 372 ALCTAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDSTVHS 410

BLAST of Bhi03G001808 vs. NCBI nr
Match: XP_038882772.1 (E3 ubiquitin-protein ligase PUB22-like [Benincasa hispida])

HSP 1 Score: 808.1 bits (2086), Expect = 3.5e-230
Identity = 424/424 (100.00%), Postives = 424/424 (100.00%), Query Frame = 0

Query: 1   MAELDQEVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVL 60
           MAELDQEVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVL
Sbjct: 1   MAELDQEVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVL 60

Query: 61  LDFDITPNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAKLSPFSQQNCL 120
           LDFDITPNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAKLSPFSQQNCL
Sbjct: 61  LDFDITPNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAKLSPFSQQNCL 120

Query: 121 RRLRSIAAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAFDDMSANLGDEALSI 180
           RRLRSIAAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAFDDMSANLGDEALSI
Sbjct: 121 RRLRSIAAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAFDDMSANLGDEALSI 180

Query: 181 LYKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQILNL 240
           LYKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQILNL
Sbjct: 181 LYKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQILNL 240

Query: 241 TTDFFIEIVQILKDQISKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVDLLLDLDSS 300
           TTDFFIEIVQILKDQISKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVDLLLDLDSS
Sbjct: 241 TTDFFIEIVQILKDQISKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVDLLLDLDSS 300

Query: 301 EKRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSVARF 360
           EKRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSVARF
Sbjct: 301 EKRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSVARF 360

Query: 361 SASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSSLRSA 420
           SASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSSLRSA
Sbjct: 361 SASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSSLRSA 420

Query: 421 YPQH 425
           YPQH
Sbjct: 421 YPQH 424

BLAST of Bhi03G001808 vs. NCBI nr
Match: XP_008441961.1 (PREDICTED: E3 ubiquitin-protein ligase PUB22 [Cucumis melo] >KAA0060143.1 E3 ubiquitin-protein ligase PUB22 [Cucumis melo var. makuwa] >TYK08456.1 E3 ubiquitin-protein ligase PUB22 [Cucumis melo var. makuwa])

HSP 1 Score: 718.0 bits (1852), Expect = 4.8e-203
Identity = 375/426 (88.03%), Postives = 403/426 (94.60%), Query Frame = 0

Query: 1   MAELD--QEVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKV 60
           MAELD   +V+DPPP+FLCPISLQIMKDPVTIASGITYDRE+IEKWLFSDKHKTCPVS +
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLLDFDITPNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAKLSPFSQQN 120
           VL DFD+TPNHTLRRVIQAWCT NA+ GVERIPTPKPPVDR+QVVRILADAKLSPFSQ+N
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 CLRRLRSIAAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAFDDMSANLGDEAL 180
           CL RLRSIAAANE+NKRCME+AGVVEFLAG+VC+STTN+E GLEDYAFDDMSAN  DEAL
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEAL 180

Query: 181 SILYKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQIL 240
            IL+KLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSR+YS+MLLNSMFEVADQIQI 
Sbjct: 181 IILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 240

Query: 241 NLTTDFFIEIVQILKDQISKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVDLLLDLD 300
           NLT DFFIEI+QILKDQISKQASKSALKLLI V SS RNRVKAV+AGAVPVL+DLLLDLD
Sbjct: 241 NLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 300

Query: 301 SSE-KRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSV 360
           SSE KRL EMILV+LD LCGCADGRA LLNHAAGIAVVSKKILR+S++GSEKAVGIL S+
Sbjct: 301 SSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSI 360

Query: 361 ARFSASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSSL 420
           ARFS SQSV+QEMVR+GVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPS+L
Sbjct: 361 ARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 420

Query: 421 RSAYPQ 424
           RSAYPQ
Sbjct: 421 RSAYPQ 426

BLAST of Bhi03G001808 vs. NCBI nr
Match: XP_023543358.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 700.7 bits (1807), Expect = 7.9e-198
Identity = 366/424 (86.32%), Postives = 396/424 (93.40%), Query Frame = 0

Query: 1   MAELDQEVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVL 60
           MAELDQE +DPPPFFLCPISLQIMKDPVT+ASGITYDRE+IEKWLFSD+HKTCPV++VVL
Sbjct: 1   MAELDQE-LDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVL 60

Query: 61  LDFDITPNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAKLSPFSQQNCL 120
             FDITPNHTLRRVIQAWCT NA  G+ERIPTPKPPVD KQVVRILADAKLSPFSQQNCL
Sbjct: 61  SSFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCL 120

Query: 121 RRLRSIAAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAF--DDMSANLGDEAL 180
           RRLRSIAA +E+NKRCMEAAG VEFLAG+VC+S TNVE   EDYA+  DD  ANLGDEAL
Sbjct: 121 RRLRSIAAGSESNKRCMEAAGAVEFLAGIVCNS-TNVEFSSEDYAYLIDDQFANLGDEAL 180

Query: 181 SILYKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQIL 240
           SILYKLQISES LKFLL+NNGGIF+STLTKILQN +Y SRAYS+MLL SMFEVADQIQIL
Sbjct: 181 SILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIL 240

Query: 241 NLTTDFFIEIVQILKDQISKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVDLLLDLD 300
           +L TDFF+EIVQILKDQISKQASKSALKLLIG+CSS RNRVKAV+AGAVPV++DLLLDLD
Sbjct: 241 SLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLD 300

Query: 301 SSEKRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSVA 360
           SSEKR  EMILV LDLLCGCADGRA +LNHAAGIAVVSKKILRVS+LGSEKAVGILCS+A
Sbjct: 301 SSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIA 360

Query: 361 RFSASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSSLR 420
           RFSAS+SV+QEM+RLGVVTKLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSCLPSSLR
Sbjct: 361 RFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLR 420

Query: 421 SAYP 423
           S++P
Sbjct: 421 SSFP 422

BLAST of Bhi03G001808 vs. NCBI nr
Match: XP_022978192.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima])

HSP 1 Score: 699.9 bits (1805), Expect = 1.3e-197
Identity = 365/424 (86.08%), Postives = 397/424 (93.63%), Query Frame = 0

Query: 1   MAELDQEVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVL 60
           MAELDQE +DPPPFFLCPISLQIMKDPVT+ASGITYDRE+IE+WLFSD+HKTCPV++VVL
Sbjct: 1   MAELDQE-LDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVL 60

Query: 61  LDFDITPNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAKLSPFSQQNCL 120
             FDITPNHT+RRVIQAWCT NA  G+ERIPTPKPP+D KQVVRILADAKLSPFSQQNCL
Sbjct: 61  SSFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCL 120

Query: 121 RRLRSIAAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAF--DDMSANLGDEAL 180
           RRLRSIAAA+E+NKRCMEAAG VEFLAG+VC+S TNVE  LEDYA+  DD  ANLGDEAL
Sbjct: 121 RRLRSIAAASESNKRCMEAAGAVEFLAGIVCNS-TNVEFSLEDYAYLIDDQFANLGDEAL 180

Query: 181 SILYKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQIL 240
           SILYKLQISES LKFLL+NNGGIF+STLTKILQN +Y SRAYS+MLL SMFEVADQIQIL
Sbjct: 181 SILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIL 240

Query: 241 NLTTDFFIEIVQILKDQISKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVDLLLDLD 300
           +L TDFFIEIVQILKDQISKQASKSALKLLIG+CSS RNRVKAV+AGA+PVL+DLLLDLD
Sbjct: 241 SLNTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLD 300

Query: 301 SSEKRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSVA 360
           SSEKR  EMILV LDLLC CADGRA +LNHAAGIAVVSKKILRVS+LGSEKAVGILCS+A
Sbjct: 301 SSEKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIA 360

Query: 361 RFSASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSSLR 420
           RFSAS+SV+QEM+RLGVVTKLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSCLPSSLR
Sbjct: 361 RFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLR 420

Query: 421 SAYP 423
           S++P
Sbjct: 421 SSFP 422

BLAST of Bhi03G001808 vs. NCBI nr
Match: KAG7033684.1 (E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 694.1 bits (1790), Expect = 7.4e-196
Identity = 364/424 (85.85%), Postives = 395/424 (93.16%), Query Frame = 0

Query: 1   MAELDQEVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVL 60
           MAELDQE +DPPPFFLCPISLQIMKDPVT+ASGITYDR++IEKWLFSD HKTCPV++VVL
Sbjct: 1   MAELDQE-LDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVL 60

Query: 61  LDFDITPNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAKLSPFSQQNCL 120
             FDITPN TLRRVIQAWCT NA  G+ERIPTPKPPVD KQVVRILADAKLSPFSQQNCL
Sbjct: 61  SSFDITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCL 120

Query: 121 RRLRSIAAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAF--DDMSANLGDEAL 180
           RRLRSIAAA+E+NKRCMEAAG VEFLAG+VC+S TNVE  LEDYA+  DD  ANLGDEAL
Sbjct: 121 RRLRSIAAASESNKRCMEAAGAVEFLAGIVCNS-TNVEFSLEDYAYLIDDQLANLGDEAL 180

Query: 181 SILYKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQIL 240
           SIL+KLQISES LKFLL+NNGGIF+STLTKILQN +Y SRAYS+MLL SMFEVADQIQIL
Sbjct: 181 SILHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIL 240

Query: 241 NLTTDFFIEIVQILKDQISKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVDLLLDLD 300
           +L TDFF+EIVQILKDQISKQASKSALKLLIG+CSS RNRVKAV+AGAVPVL+DLLLDLD
Sbjct: 241 SLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLD 300

Query: 301 SSEKRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSVA 360
           SSEKR  EMILV LDLLCGCADGRA +LNHAAGIA+VSKKILRVS+LGSEKAVGILCS+A
Sbjct: 301 SSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIA 360

Query: 361 RFSASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSSLR 420
           RFSAS+SV+QEM+RLGVVTKLCFLLQV GAG KAKEKAKEILKLHGRTWRNSSCLPSSLR
Sbjct: 361 RFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLR 420

Query: 421 SAYP 423
           S++P
Sbjct: 421 SSFP 422

BLAST of Bhi03G001808 vs. ExPASy TrEMBL
Match: A0A5A7UVZ1 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold654G00730 PE=4 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 2.3e-203
Identity = 375/426 (88.03%), Postives = 403/426 (94.60%), Query Frame = 0

Query: 1   MAELD--QEVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKV 60
           MAELD   +V+DPPP+FLCPISLQIMKDPVTIASGITYDRE+IEKWLFSDKHKTCPVS +
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLLDFDITPNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAKLSPFSQQN 120
           VL DFD+TPNHTLRRVIQAWCT NA+ GVERIPTPKPPVDR+QVVRILADAKLSPFSQ+N
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 CLRRLRSIAAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAFDDMSANLGDEAL 180
           CL RLRSIAAANE+NKRCME+AGVVEFLAG+VC+STTN+E GLEDYAFDDMSAN  DEAL
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEAL 180

Query: 181 SILYKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQIL 240
            IL+KLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSR+YS+MLLNSMFEVADQIQI 
Sbjct: 181 IILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 240

Query: 241 NLTTDFFIEIVQILKDQISKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVDLLLDLD 300
           NLT DFFIEI+QILKDQISKQASKSALKLLI V SS RNRVKAV+AGAVPVL+DLLLDLD
Sbjct: 241 NLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 300

Query: 301 SSE-KRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSV 360
           SSE KRL EMILV+LD LCGCADGRA LLNHAAGIAVVSKKILR+S++GSEKAVGIL S+
Sbjct: 301 SSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSI 360

Query: 361 ARFSASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSSL 420
           ARFS SQSV+QEMVR+GVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPS+L
Sbjct: 361 ARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 420

Query: 421 RSAYPQ 424
           RSAYPQ
Sbjct: 421 RSAYPQ 426

BLAST of Bhi03G001808 vs. ExPASy TrEMBL
Match: A0A1S3B4K2 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485961 PE=4 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 2.3e-203
Identity = 375/426 (88.03%), Postives = 403/426 (94.60%), Query Frame = 0

Query: 1   MAELD--QEVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKV 60
           MAELD   +V+DPPP+FLCPISLQIMKDPVTIASGITYDRE+IEKWLFSDKHKTCPVS +
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLLDFDITPNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAKLSPFSQQN 120
           VL DFD+TPNHTLRRVIQAWCT NA+ GVERIPTPKPPVDR+QVVRILADAKLSPFSQ+N
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 CLRRLRSIAAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAFDDMSANLGDEAL 180
           CL RLRSIAAANE+NKRCME+AGVVEFLAG+VC+STTN+E GLEDYAFDDMSAN  DEAL
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEAL 180

Query: 181 SILYKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQIL 240
            IL+KLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSR+YS+MLLNSMFEVADQIQI 
Sbjct: 181 IILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 240

Query: 241 NLTTDFFIEIVQILKDQISKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVDLLLDLD 300
           NLT DFFIEI+QILKDQISKQASKSALKLLI V SS RNRVKAV+AGAVPVL+DLLLDLD
Sbjct: 241 NLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 300

Query: 301 SSE-KRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSV 360
           SSE KRL EMILV+LD LCGCADGRA LLNHAAGIAVVSKKILR+S++GSEKAVGIL S+
Sbjct: 301 SSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSI 360

Query: 361 ARFSASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSSL 420
           ARFS SQSV+QEMVR+GVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPS+L
Sbjct: 361 ARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 420

Query: 421 RSAYPQ 424
           RSAYPQ
Sbjct: 421 RSAYPQ 426

BLAST of Bhi03G001808 vs. ExPASy TrEMBL
Match: A0A6J1IKG7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478249 PE=4 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 6.5e-198
Identity = 365/424 (86.08%), Postives = 397/424 (93.63%), Query Frame = 0

Query: 1   MAELDQEVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVL 60
           MAELDQE +DPPPFFLCPISLQIMKDPVT+ASGITYDRE+IE+WLFSD+HKTCPV++VVL
Sbjct: 1   MAELDQE-LDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVL 60

Query: 61  LDFDITPNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAKLSPFSQQNCL 120
             FDITPNHT+RRVIQAWCT NA  G+ERIPTPKPP+D KQVVRILADAKLSPFSQQNCL
Sbjct: 61  SSFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCL 120

Query: 121 RRLRSIAAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAF--DDMSANLGDEAL 180
           RRLRSIAAA+E+NKRCMEAAG VEFLAG+VC+S TNVE  LEDYA+  DD  ANLGDEAL
Sbjct: 121 RRLRSIAAASESNKRCMEAAGAVEFLAGIVCNS-TNVEFSLEDYAYLIDDQFANLGDEAL 180

Query: 181 SILYKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQIL 240
           SILYKLQISES LKFLL+NNGGIF+STLTKILQN +Y SRAYS+MLL SMFEVADQIQIL
Sbjct: 181 SILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIL 240

Query: 241 NLTTDFFIEIVQILKDQISKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVDLLLDLD 300
           +L TDFFIEIVQILKDQISKQASKSALKLLIG+CSS RNRVKAV+AGA+PVL+DLLLDLD
Sbjct: 241 SLNTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLD 300

Query: 301 SSEKRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSVA 360
           SSEKR  EMILV LDLLC CADGRA +LNHAAGIAVVSKKILRVS+LGSEKAVGILCS+A
Sbjct: 301 SSEKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIA 360

Query: 361 RFSASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSSLR 420
           RFSAS+SV+QEM+RLGVVTKLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSCLPSSLR
Sbjct: 361 RFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLR 420

Query: 421 SAYP 423
           S++P
Sbjct: 421 SSFP 422

BLAST of Bhi03G001808 vs. ExPASy TrEMBL
Match: A0A6J1GGQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453790 PE=4 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 8.0e-196
Identity = 362/424 (85.38%), Postives = 395/424 (93.16%), Query Frame = 0

Query: 1   MAELDQEVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVL 60
           MAELDQE +DPPPFFLCPISLQIMKDPVT+ASGITYDRE+IE+WLFSD+HKTCP+++VVL
Sbjct: 1   MAELDQE-LDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVL 60

Query: 61  LDFDITPNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAKLSPFSQQNCL 120
            +FDITPNHTLRRVIQAWCT NA  GVERIPTPKPPVD KQVVRILADAKLS FSQQNCL
Sbjct: 61  SNFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCL 120

Query: 121 RRLRSIAAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAF--DDMSANLGDEAL 180
           RRL+SIAAA+E+NKRCMEAAG VEFLAG+VC+S TNVE  LEDYA+  DD  ANLG EAL
Sbjct: 121 RRLQSIAAASESNKRCMEAAGAVEFLAGIVCNS-TNVEFSLEDYAYLIDDQLANLGFEAL 180

Query: 181 SILYKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQIL 240
           SILYKLQISES LKFLL+NNGGIF+STLTKILQN +Y SR YS+MLL SMFEVADQIQIL
Sbjct: 181 SILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQIL 240

Query: 241 NLTTDFFIEIVQILKDQISKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVDLLLDLD 300
           +L TDFF+EIVQILKDQISKQASKSALKLLIG+CSS RNRVKAV+AGAVPVL+DLLLDLD
Sbjct: 241 SLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLD 300

Query: 301 SSEKRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSVA 360
           SSEKR  EMILV LDLLCGCADGRA +LNHAAGIA+VSKKILRVS+LGSEKAVGILCS+A
Sbjct: 301 SSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIA 360

Query: 361 RFSASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSSLR 420
           RFSAS+SV+QEM+RLGVVTKLCFLLQV GAG KAKEKAKEILKLHGRTWRNSSCLPSSLR
Sbjct: 361 RFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLR 420

Query: 421 SAYP 423
           S++P
Sbjct: 421 SSFP 422

BLAST of Bhi03G001808 vs. ExPASy TrEMBL
Match: A0A0A0L1F5 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G194780 PE=4 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 9.8e-194
Identity = 363/425 (85.41%), Postives = 392/425 (92.24%), Query Frame = 0

Query: 1   MAELDQEVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVL 60
           MAELDQ V+DPPP+FLCPIS QIMKDPVT+ASGITYDRE+IEKWL S KH TCPVS +VL
Sbjct: 1   MAELDQ-VLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVL 60

Query: 61  LDFDITPNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAKLSPFSQQNCL 120
             F ITPNHTLRRVIQAWCT NA+ GVERIPTPKPPVDR+QVVRILA A LSPFSQ+N L
Sbjct: 61  SHFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSL 120

Query: 121 RRLRSIAAANETNKRCMEAAGVVEFLAGLVC-SSTTNVESGLEDYAFDDMSANLGDEALS 180
            RLRSIA ANE+NKRCME+AGVVEFLAG+VC +STTN+E GLEDYAFDDMSAN  DEAL 
Sbjct: 121 CRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEALL 180

Query: 181 ILYKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQILN 240
           IL+KLQ+SESSLKFLLSNNGGIF+STLTKILQNRSYSSR+YS+MLLNSMF+VADQIQI N
Sbjct: 181 ILHKLQVSESSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFQVADQIQIQN 240

Query: 241 LTTDFFIEIVQILKDQISKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVDLLLDLDS 300
           LT DFF EI+QILKDQISKQASKSALKLLI V SS RNRVKAV+AGAVPVL+DLLLDLDS
Sbjct: 241 LTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDS 300

Query: 301 SE-KRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSVA 360
           SE KRL EMILV+LDLLC CADGRA LLNHAAGIAVVSKKILRVSS+GSEKAVGIL SVA
Sbjct: 301 SENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVA 360

Query: 361 RFSASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSSLR 420
           +FS SQSV+QEMVR+GVVTKLCF+LQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPS+LR
Sbjct: 361 KFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLR 420

Query: 421 SAYPQ 424
           SAYPQ
Sbjct: 421 SAYPQ 424

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G52450.14.9e-12153.90plant U-box 22 [more]
AT2G35930.11.1e-11753.11plant U-box 23 [more]
AT3G11840.12.2e-6537.39plant U-box 24 [more]
AT3G19380.11.0e-4934.61plant U-box 25 [more]
AT1G49780.13.6e-4733.49plant U-box 26 [more]
Match NameE-valueIdentityDescription
Q9SVC66.9e-12053.90E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q84TG31.6e-11653.11E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SF153.1e-6437.39E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ... [more]
Q9LT791.4e-4834.61U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9FXA45.0e-4633.49U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Match NameE-valueIdentityDescription
XP_038882772.13.5e-230100.00E3 ubiquitin-protein ligase PUB22-like [Benincasa hispida][more]
XP_008441961.14.8e-20388.03PREDICTED: E3 ubiquitin-protein ligase PUB22 [Cucumis melo] >KAA0060143.1 E3 ubi... [more]
XP_023543358.17.9e-19886.32E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo][more]
XP_022978192.11.3e-19786.08E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima][more]
KAG7033684.17.4e-19685.85E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
A0A5A7UVZ12.3e-20388.03RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3B4K22.3e-20388.03RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485961 PE=4 ... [more]
A0A6J1IKG76.5e-19886.08RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478249 P... [more]
A0A6J1GGQ88.0e-19685.38RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453790... [more]
A0A0A0L1F59.8e-19485.41RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G194780 PE... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 14..78
e-value: 2.8E-32
score: 123.3
IPR003613U box domainPFAMPF04564U-boxcoord: 12..82
e-value: 3.4E-19
score: 68.8
IPR003613U box domainPROSITEPS51698U_BOXcoord: 10..85
score: 42.664577
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 2..89
e-value: 5.5E-27
score: 95.6
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 99..409
e-value: 4.5E-15
score: 57.3
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 8..422
NoneNo IPR availablePANTHERPTHR22849:SF132E3 UBIQUITIN-PROTEIN LIGASE PUB23coord: 8..422
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 15..57
e-value: 3.58509E-21
score: 83.8222
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 3..85
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 103..384

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M001808Bhi03M001808mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity