Bhi03G001707 (gene) Wax gourd (B227) v1

Overview
NameBhi03G001707
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionPyruvate decarboxylase
Locationchr3: 47039075 .. 47040516 (+)
RNA-Seq ExpressionBhi03G001707
SyntenyBhi03G001707
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGTGGAGAAGGCAGCTCGACTTCTAAATAGCGCGGTTAAACCCGTCCTAGTAGGAGGAAAAAAGTTACGACCTGCCAAAGCACAAGCCGTATTCGTAGAGCTAGCCAATGCTTGTGAGTACGCGGTTGCTGTTACACCATCAGCAAAAGGAATGTTCCCTGAACAGCACCCTCATTTCATTGGAACTTATTGGGGAGTTCTCAGCACCGCCTTCTGCGGCGAGACGGTGGAAATTGCCGACGCCTCAATCTTTGTCGGAGCTATGTTTGATGAATTGGAAACCGTCGGCTTCTCGCTTTCATACAAGAAGAACAATGCCATTATCATAGAAACCGATCACGTTGTGTTTCCAAATGGGCCGAGTTTTGGGCCGATTTTAATGAAGGACTTTCTTTGGGCCCTTGCTAAGCGACTCAAGCCCAATGCAAAGGCTTATGAGAATTATCGTCGGATTTATATTCCAGAGTCAAGCCCTGCCAAGTCAGAGCCGGGCGAAGCTTTACGAGTCAACGTTCTTTTCAAACATATTCAGAAAATGTTGTCAAGTAACATGGTTGTAATTTCTGAAGCGGGTGATTCCTGGTTTCACTGTCAAAAACTTGAATTACCACGATCATGCGGGTAAAATTCTTACAAAATTTTTAACTCAAATGGGTTTAAATTATTATGAGTATAATTTTAGAATAACGTTTGGAGAAATGTATATAGGTATGAGATGCAATTGGTATATGCATCTATTGGATGGTCTTTAGGTGCTACTCTTGGATATGCACAAGCTTCATTAGATGATCGTGTGGTTCTTTGCATTGGTGATGGAAGCTTTCAAGTTAGTTTTCATTTTAATGAATTCTAATTCTATTTTTGTCATTGAATTTTTTTTTTTATCCAAAATTGTTTTTGTTAGAAATAGATTGAACGATATTGTGGTAATTTGGCAGATGGCTCCACAAGATTTGTCAACAATGTTAAGATTAGGTCAAAGGAACATAATATTTTTGATAAATAATGGTGGATACACAATTGAAGTTGAGATTCATGATGGCCCTTACAATGTCATCAAGAATTGGGACTACACTGCTCTTGTTGATGCAATGCATAATCACAATGGCAATTGTTGGACGGCCAAGGTAAATTCTCAAATAACTTTTTTTTTTTCATTTTGATTGAACAACCATATGTGGACGGTTAAAAAATGACAACTATTATTATAGTTGACGTTATTTTCATTACTTGAGATTTTGATAAGTTTTGACTTTCAACATAAAGGTTCAAACAGAGGAAGAATTAGTGAAAGCAATTGAAATTGCAAAGGGAGACCGAAAGGACTGTTTGTGCTTTATAGAGGCTATCGTTCATAAAGATGATACAAGTAAAGAGTTACTTGAATTTGGGAACAAACTTGCTGCTATCAATAGTCGTCCTCCAAAATCTTTATAG

mRNA sequence

ATGGCAGTGGAGAAGGCAGCTCGACTTCTAAATAGCGCGGTTAAACCCGTCCTAGTAGGAGGAAAAAAGTTACGACCTGCCAAAGCACAAGCCGTATTCGTAGAGCTAGCCAATGCTTGTGAGTACGCGGTTGCTGTTACACCATCAGCAAAAGGAATGTTCCCTGAACAGCACCCTCATTTCATTGGAACTTATTGGGGAGTTCTCAGCACCGCCTTCTGCGGCGAGACGGTGGAAATTGCCGACGCCTCAATCTTTGTCGGAGCTATGTTTGATGAATTGGAAACCGTCGGCTTCTCGCTTTCATACAAGAAGAACAATGCCATTATCATAGAAACCGATCACGTTGTGTTTCCAAATGGGCCGAGTTTTGGGCCGATTTTAATGAAGGACTTTCTTTGGGCCCTTGCTAAGCGACTCAAGCCCAATGCAAAGGCTTATGAGAATTATCGTCGGATTTATATTCCAGAGTCAAGCCCTGCCAAGTCAGAGCCGGGCGAAGCTTTACGAGTCAACGTTCTTTTCAAACATATTCAGAAAATGTTGTCAAGTAACATGGTTGTAATTTCTGAAGCGGGTGCTACTCTTGGATATGCACAAGCTTCATTAGATGATCGTGTGGTTCTTTGCATTGGTGATGGAAGCTTTCAAATGGCTCCACAAGATTTGTCAACAATGTTAAGATTAGGTCAAAGGAACATAATATTTTTGATAAATAATGGTGGATACACAATTGAAGTTGAGATTCATGATGGCCCTTACAATGTCATCAAGAATTGGGACTACACTGCTCTTGTTGATGCAATGCATAATCACAATGGCAATTGTTGGACGGCCAAGGTTCAAACAGAGGAAGAATTAGTGAAAGCAATTGAAATTGCAAAGGGAGACCGAAAGGACTGTTTGTGCTTTATAGAGGCTATCGTTCATAAAGATGATACAAGTAAAGAGTTACTTGAATTTGGGAACAAACTTGCTGCTATCAATAGTCGTCCTCCAAAATCTTTATAG

Coding sequence (CDS)

ATGGCAGTGGAGAAGGCAGCTCGACTTCTAAATAGCGCGGTTAAACCCGTCCTAGTAGGAGGAAAAAAGTTACGACCTGCCAAAGCACAAGCCGTATTCGTAGAGCTAGCCAATGCTTGTGAGTACGCGGTTGCTGTTACACCATCAGCAAAAGGAATGTTCCCTGAACAGCACCCTCATTTCATTGGAACTTATTGGGGAGTTCTCAGCACCGCCTTCTGCGGCGAGACGGTGGAAATTGCCGACGCCTCAATCTTTGTCGGAGCTATGTTTGATGAATTGGAAACCGTCGGCTTCTCGCTTTCATACAAGAAGAACAATGCCATTATCATAGAAACCGATCACGTTGTGTTTCCAAATGGGCCGAGTTTTGGGCCGATTTTAATGAAGGACTTTCTTTGGGCCCTTGCTAAGCGACTCAAGCCCAATGCAAAGGCTTATGAGAATTATCGTCGGATTTATATTCCAGAGTCAAGCCCTGCCAAGTCAGAGCCGGGCGAAGCTTTACGAGTCAACGTTCTTTTCAAACATATTCAGAAAATGTTGTCAAGTAACATGGTTGTAATTTCTGAAGCGGGTGCTACTCTTGGATATGCACAAGCTTCATTAGATGATCGTGTGGTTCTTTGCATTGGTGATGGAAGCTTTCAAATGGCTCCACAAGATTTGTCAACAATGTTAAGATTAGGTCAAAGGAACATAATATTTTTGATAAATAATGGTGGATACACAATTGAAGTTGAGATTCATGATGGCCCTTACAATGTCATCAAGAATTGGGACTACACTGCTCTTGTTGATGCAATGCATAATCACAATGGCAATTGTTGGACGGCCAAGGTTCAAACAGAGGAAGAATTAGTGAAAGCAATTGAAATTGCAAAGGGAGACCGAAAGGACTGTTTGTGCTTTATAGAGGCTATCGTTCATAAAGATGATACAAGTAAAGAGTTACTTGAATTTGGGAACAAACTTGCTGCTATCAATAGTCGTCCTCCAAAATCTTTATAG

Protein sequence

MAVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPHFIGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPNGPSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQKMLSSNMVVISEAGATLGYAQASLDDRVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMHNHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAAINSRPPKSL
Homology
BLAST of Bhi03G001707 vs. TAIR 10
Match: AT5G54960.1 (pyruvate decarboxylase-2 )

HSP 1 Score: 433.7 bits (1114), Expect = 1.3e-121
Identity = 218/363 (60.06%), Postives = 257/363 (70.80%), Query Frame = 0

Query: 2   AVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPHF 61
           AVE AA  LN AVKPVLVGG K+R AKA   FVELA+A  Y +AV PSAKG  PE H HF
Sbjct: 242 AVEAAAEFLNKAVKPVLVGGPKMRVAKAADAFVELADASGYGLAVMPSAKGQVPEHHKHF 301

Query: 62  IGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPNG 121
           IGTYWG +STAFC E VE ADA +F G +F++  +VG+SL  KK  AII++ D V   NG
Sbjct: 302 IGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKKEKAIIVQPDRVTIGNG 361

Query: 122 PSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQKM 181
           P+FG +LMKDFL  LAKR+K N  +YENY RIY+PE  P +  P E+LRVNVLF+HIQ M
Sbjct: 362 PAFGCVLMKDFLSELAKRIKHNNTSYENYHRIYVPEGKPLRDNPNESLRVNVLFQHIQNM 421

Query: 182 LSSNMVVISE-------------------------------AGATLGYAQASLDDRVVLC 241
           LSS   V++E                                GATLGYAQA  + RV+ C
Sbjct: 422 LSSESAVLAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSVGATLGYAQAMPNRRVIAC 481

Query: 242 IGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMH 301
           IGDGSFQ+  QD+STM+R GQ+ IIFLINNGGYTIEVEIHDGPYNVIKNW+YTA V+A+H
Sbjct: 482 IGDGSFQVTAQDVSTMIRCGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTAFVEAIH 541

Query: 302 NHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAAINS 334
           N  G CWTAKV+ EEELVKAI  A  + K+  CFIE IVHKDDTSKELLE+G++++A NS
Sbjct: 542 NGEGKCWTAKVRCEEELVKAINTATNEEKESFCFIEVIVHKDDTSKELLEWGSRVSAANS 601

BLAST of Bhi03G001707 vs. TAIR 10
Match: AT4G33070.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 429.9 bits (1104), Expect = 1.9e-120
Identity = 218/363 (60.06%), Postives = 257/363 (70.80%), Query Frame = 0

Query: 2   AVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPHF 61
           AVE     LN AVKPV+VGG KLR AKA   FVELA+A  YA+A+ PSAKG  PE HPHF
Sbjct: 242 AVEATLEFLNKAVKPVMVGGPKLRVAKACDAFVELADASGYALAMMPSAKGFVPEHHPHF 301

Query: 62  IGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPNG 121
           IGTYWG +ST FC E VE ADA IF G +F++  +VG+SL  KK  AI+++ D +   NG
Sbjct: 302 IGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYSLLLKKEKAIVVQPDRITVANG 361

Query: 122 PSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQKM 181
           P+FG ILM DF   L+KR+K N  AYENY RI++PE  P K E  E LRVN +F+HIQKM
Sbjct: 362 PTFGCILMSDFFRELSKRVKRNETAYENYHRIFVPEGKPLKCESREPLRVNTMFQHIQKM 421

Query: 182 LSSNMVVISE-------------------------------AGATLGYAQASLDDRVVLC 241
           LSS   VI+E                                GATLGYAQAS + RV+  
Sbjct: 422 LSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQASPEKRVLAF 481

Query: 242 IGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMH 301
           IGDGSFQ+  QD+STMLR GQ+ IIFLINNGGYTIEVEIHDGPYNVIKNW+YT LVDA+H
Sbjct: 482 IGDGSFQVTVQDISTMLRNGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIH 541

Query: 302 NHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAAINS 334
           N  GNCWTAKV+ EEELV+AI  A  ++KDCLCFIE I+HKDDTSKELLE+G++++A NS
Sbjct: 542 NGEGNCWTAKVRYEEELVEAITTATTEKKDCLCFIEVILHKDDTSKELLEWGSRVSAANS 601

BLAST of Bhi03G001707 vs. TAIR 10
Match: AT5G01320.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 425.6 bits (1093), Expect = 3.6e-119
Identity = 215/363 (59.23%), Postives = 255/363 (70.25%), Query Frame = 0

Query: 2   AVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPHF 61
           AVE     LN AVKPV+VGG KLR AKA   F+ELA+A  Y +AV PS KG+ PE HPHF
Sbjct: 238 AVEATLEFLNKAVKPVMVGGPKLRVAKASEAFLELADASGYPLAVMPSTKGLVPENHPHF 297

Query: 62  IGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPNG 121
           IGTYWG +ST FC E VE ADA IF G +F++  +VG+SL  KK  AII+  D VV  NG
Sbjct: 298 IGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYSLLLKKEKAIIVHPDRVVVANG 357

Query: 122 PSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQKM 181
           P+FG +LM DF   LAKR+K N  AYENY RI++PE  P K +PGE LRVN +F+HIQKM
Sbjct: 358 PTFGCVLMSDFFRELAKRVKRNETAYENYERIFVPEGKPLKCKPGEPLRVNAMFQHIQKM 417

Query: 182 LSSNMVVISE-------------------------------AGATLGYAQASLDDRVVLC 241
           LSS   VI+E                                GATLGYAQA+ + RV+  
Sbjct: 418 LSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQATPEKRVLSF 477

Query: 242 IGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMH 301
           IGDGSFQ+  QD+STM+R GQ+ IIFLINNGGYTIEVEIHDGPYNVIKNW+YT LVDA+H
Sbjct: 478 IGDGSFQVTAQDISTMIRNGQKAIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIH 537

Query: 302 NHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAAINS 334
           N  G CWT KV+ EEELV+AI+ A  ++KD LCFIE IVHKDDTSKELLE+G++++A N 
Sbjct: 538 NGEGKCWTTKVRYEEELVEAIKTATTEKKDSLCFIEVIVHKDDTSKELLEWGSRVSAANG 597

BLAST of Bhi03G001707 vs. TAIR 10
Match: AT5G01330.1 (pyruvate decarboxylase-3 )

HSP 1 Score: 422.5 bits (1085), Expect = 3.1e-118
Identity = 214/363 (58.95%), Postives = 254/363 (69.97%), Query Frame = 0

Query: 2   AVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPHF 61
           AVE     LN AVKPV+VGG KLR AKA+  FVELA+A  Y VAV PSAKG  PE HPHF
Sbjct: 227 AVEATLEFLNKAVKPVMVGGPKLRVAKARDAFVELADASGYPVAVMPSAKGFVPENHPHF 286

Query: 62  IGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPNG 121
           IGTYWG +ST FC E VE ADA IF G +F++  +VG+SL  KK  AII+  D VV  NG
Sbjct: 287 IGTYWGAVSTLFCSEIVESADAYIFAGPIFNDYSSVGYSLLLKKEKAIIVHPDSVVVANG 346

Query: 122 PSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQKM 181
           P+FG + M +F   LAKR+KPN  AYENY RI++PE  P K +P E LR+N +F+HIQKM
Sbjct: 347 PTFGCVRMSEFFRELAKRVKPNKTAYENYHRIFVPEGKPLKCKPREPLRINAMFQHIQKM 406

Query: 182 LSSNMVVISE-------------------------------AGATLGYAQASLDDRVVLC 241
           LS+   VI+E                                GATLGYAQA+ + RV+  
Sbjct: 407 LSNETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQATPEKRVLSF 466

Query: 242 IGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMH 301
           IGDGSFQ+  QD+STM+R GQ+ IIFLINNGGYTIEVEIHDGPYNVIKNW+YT LVDA+H
Sbjct: 467 IGDGSFQVTAQDVSTMIRNGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIH 526

Query: 302 NHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAAINS 334
           N  G CWT KV+ EEELV+AI  A  ++KD LCFIE IVHKDDTSKELLE+G++++A N 
Sbjct: 527 NGEGKCWTTKVRYEEELVEAINTATLEKKDSLCFIEVIVHKDDTSKELLEWGSRVSAANG 586

BLAST of Bhi03G001707 vs. TAIR 10
Match: AT5G17380.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 43.1 bits (100), Expect = 5.1e-04
Identity = 63/289 (21.80%), Postives = 102/289 (35.29%), Query Frame = 0

Query: 3   VEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPHFI 62
           +E A  LL  A +P++V GK    ++A+    +L          TP  KG+ P+ H  F 
Sbjct: 206 IESAVSLLRKAERPLIVFGKGAAYSRAEDELKKLVEITGIPFLPTPMGKGLLPDTH-EFS 265

Query: 63  GTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIII--ETDHVVFPN 122
            T    L+   C       D ++ VGA  + L   G S  + K+   I+   ++  +   
Sbjct: 266 ATAARSLAIGKC-------DVALVVGARLNWLLHFGESPKWDKDVKFILVDVSEEEIELR 325

Query: 123 GPSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRV--------- 182
            P  G +     +  L  R   +          ++   S    E GE + +         
Sbjct: 326 KPHLGIVGDAKTVIGLLNREIKDDPFCLGKSNSWVESISKKAKENGEKMEIQLAKDVVPF 385

Query: 183 ------NVLFKHIQKMLSSNMVVISEA----------------------------GATLG 242
                  ++   I  +   + VV+SE                             G  LG
Sbjct: 386 NFLTPMRIIRDAILAVEGPSPVVVSEGANTMDVGRSVLVQKEPRTRLDAGTWGTMGVGLG 445

BLAST of Bhi03G001707 vs. ExPASy Swiss-Prot
Match: P51851 (Pyruvate decarboxylase 2 (Fragment) OS=Pisum sativum OX=3888 GN=PDC2 PE=2 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 4.0e-123
Identity = 228/363 (62.81%), Postives = 261/363 (71.90%), Query Frame = 0

Query: 2   AVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPHF 61
           AVE AA  LN AVKPVLVGG KLR AKA   FVELA+A  YA+AV PSAKGM PE HPHF
Sbjct: 40  AVEAAAEFLNKAVKPVLVGGPKLRVAKASDAFVELADASGYALAVMPSAKGMVPEHHPHF 99

Query: 62  IGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPNG 121
           IGTYWG +STAFC E VE ADA +F G +F++  +VG+SL  KK  AII+  D VV  NG
Sbjct: 100 IGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKKEKAIIVMPDRVVIANG 159

Query: 122 PSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQKM 181
           P+FG +LM DFL ALAKRLK N  AYENY RI++P+ +P KS   E LRVNV+F+HIQKM
Sbjct: 160 PAFGCVLMNDFLKALAKRLKHNNVAYENYHRIFVPDGTPLKSASKEPLRVNVMFQHIQKM 219

Query: 182 LSSNMVVISE-------------------------------AGATLGYAQASLDDRVVLC 241
           LSS   VI+E                                GATLGYAQA  + RV+ C
Sbjct: 220 LSSETAVIAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSVGATLGYAQAVPEKRVIAC 279

Query: 242 IGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMH 301
           IGDGSFQ+  QD+STMLR GQ+ IIFLINNGGYTIEVEIHDGPYNVIKNW+YT LVDA+H
Sbjct: 280 IGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIH 339

Query: 302 NHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAAINS 334
           N  G CWT KV  EEELV+AI  A G +KD LCFIE IVHKDDTSKELLE+G++++A NS
Sbjct: 340 NGEGKCWTTKVFCEEELVEAIAKATGPKKDSLCFIEVIVHKDDTSKELLEWGSRVSAANS 399

BLAST of Bhi03G001707 vs. ExPASy Swiss-Prot
Match: A2Y5L9 (Pyruvate decarboxylase 1 OS=Oryza sativa subsp. indica OX=39946 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 4.5e-122
Identity = 217/363 (59.78%), Postives = 263/363 (72.45%), Query Frame = 0

Query: 2   AVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPHF 61
           AVE AA  LN AVKPV+VGG K+R AKA+  F  +A +  Y +AV PSAKG+ PE HP F
Sbjct: 240 AVEAAADFLNKAVKPVMVGGPKIRVAKAKKAFAGIAESSGYPIAVMPSAKGLVPEHHPRF 299

Query: 62  IGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPNG 121
           IGTYWG +ST FC E VE ADA +F G +F++  +VG+SL  K+  A+I++ D VV  NG
Sbjct: 300 IGTYWGAVSTTFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVQPDRVVVGNG 359

Query: 122 PSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQKM 181
           P+FG ILM +FL ALAKRL  N  AY+NYRRI+IP+  P   +P E LRVN+LFKHI++M
Sbjct: 360 PAFGCILMTEFLDALAKRLDRNTTAYDNYRRIFIPDREPPNGQPDEPLRVNILFKHIKEM 419

Query: 182 LSSNMVVISE-------------------------------AGATLGYAQASLDDRVVLC 241
           LS +  VI+E                                GATLGYAQA+ D RV+ C
Sbjct: 420 LSGDTAVIAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGATLGYAQAAKDKRVISC 479

Query: 242 IGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMH 301
           IGDGSFQM  QD+STMLR GQ++IIFLINNGGYTIEVEIHDGPYNVIKNWDYT L+DA+H
Sbjct: 480 IGDGSFQMTAQDVSTMLRCGQKSIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLIDAIH 539

Query: 302 NHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAAINS 334
           N +GNCWT KV+TEEEL++AI  A G +KDCLCFIE IVHKDDTSKELLE+G++++A NS
Sbjct: 540 NSDGNCWTKKVRTEEELIEAIATATGAKKDCLCFIEIIVHKDDTSKELLEWGSRVSAANS 599

BLAST of Bhi03G001707 vs. ExPASy Swiss-Prot
Match: Q0DHF6 (Pyruvate decarboxylase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 1.3e-121
Identity = 217/363 (59.78%), Postives = 262/363 (72.18%), Query Frame = 0

Query: 2   AVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPHF 61
           AVE AA  LN AVKPV+VGG K+R AKA+  F  +A +  Y  AV PSAKG+ PE HP F
Sbjct: 240 AVEAAADFLNKAVKPVMVGGPKIRVAKAKKAFAGIAESSGYPFAVMPSAKGLVPEHHPRF 299

Query: 62  IGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPNG 121
           IGTYWG +ST FC E VE ADA +F G +F++  +VG+SL  K+  A+I++ D VV  NG
Sbjct: 300 IGTYWGAVSTTFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVQPDRVVVGNG 359

Query: 122 PSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQKM 181
           P+FG ILM +FL ALAKRL  N  AY+NYRRI+IP+  P   +P E LRVN+LFKHI++M
Sbjct: 360 PAFGCILMTEFLDALAKRLDRNTTAYDNYRRIFIPDREPPNGQPDEPLRVNILFKHIKEM 419

Query: 182 LSSNMVVISE-------------------------------AGATLGYAQASLDDRVVLC 241
           LS +  VI+E                                GATLGYAQA+ D RV+ C
Sbjct: 420 LSGDTAVIAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGATLGYAQAAKDKRVISC 479

Query: 242 IGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMH 301
           IGDGSFQM  QD+STMLR GQ++IIFLINNGGYTIEVEIHDGPYNVIKNWDYT L+DA+H
Sbjct: 480 IGDGSFQMTAQDVSTMLRCGQKSIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLIDAIH 539

Query: 302 NHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAAINS 334
           N +GNCWT KV+TEEEL++AI  A G +KDCLCFIE IVHKDDTSKELLE+G++++A NS
Sbjct: 540 NSDGNCWTKKVRTEEELIEAIATATGAKKDCLCFIEIIVHKDDTSKELLEWGSRVSAANS 599

BLAST of Bhi03G001707 vs. ExPASy Swiss-Prot
Match: A2XFI3 (Pyruvate decarboxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=PDC2 PE=2 SV=2)

HSP 1 Score: 435.6 bits (1119), Expect = 4.9e-121
Identity = 214/363 (58.95%), Postives = 261/363 (71.90%), Query Frame = 0

Query: 2   AVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPHF 61
           AVE     LN AVKPVLVGG KLR AKA   FV+L +A  YA AV PSAKG+ PE HPHF
Sbjct: 241 AVEATVEFLNKAVKPVLVGGPKLRVAKAGKAFVDLVDASGYAYAVMPSAKGLVPETHPHF 300

Query: 62  IGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPNG 121
           IGTYWG +STAFC E VE ADA +F G +F++  +VG+S   KK+ AII++ + V+  NG
Sbjct: 301 IGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSFLLKKDKAIIVQPERVIVGNG 360

Query: 122 PSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQKM 181
           P+FG ++MK+FL  LAKR+  N  AYENY+RI++PE  P +SEP E LRVNVLFKH+QKM
Sbjct: 361 PAFGCVMMKEFLSELAKRVNKNTTAYENYKRIFVPEGQPLESEPNEPLRVNVLFKHVQKM 420

Query: 182 LSSNMVVISE-------------------------------AGATLGYAQASLDDRVVLC 241
           L+S+  VI+E                                GA LGYAQ + D RV+ C
Sbjct: 421 LNSDSAVIAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSVGALLGYAQGAKDKRVIAC 480

Query: 242 IGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMH 301
           IGDGSFQ+  QD+STM+R  Q +IIFLINNGGYTIEVEIHDGPYNVIKNW+YT LVDA+H
Sbjct: 481 IGDGSFQVTAQDVSTMIRCAQNSIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIH 540

Query: 302 NHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAAINS 334
           N  G CWT+KV+ EEEL +AI +A G++KDCLCFIE I HKDDTSKELLE+G++++A NS
Sbjct: 541 NGEGKCWTSKVKCEEELTEAIGMALGEKKDCLCFIEVIAHKDDTSKELLEWGSRVSAANS 600

BLAST of Bhi03G001707 vs. ExPASy Swiss-Prot
Match: Q9FFT4 (Pyruvate decarboxylase 2 OS=Arabidopsis thaliana OX=3702 GN=PDC2 PE=2 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 1.9e-120
Identity = 218/363 (60.06%), Postives = 257/363 (70.80%), Query Frame = 0

Query: 2   AVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPHF 61
           AVE AA  LN AVKPVLVGG K+R AKA   FVELA+A  Y +AV PSAKG  PE H HF
Sbjct: 242 AVEAAAEFLNKAVKPVLVGGPKMRVAKAADAFVELADASGYGLAVMPSAKGQVPEHHKHF 301

Query: 62  IGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPNG 121
           IGTYWG +STAFC E VE ADA +F G +F++  +VG+SL  KK  AII++ D V   NG
Sbjct: 302 IGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKKEKAIIVQPDRVTIGNG 361

Query: 122 PSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQKM 181
           P+FG +LMKDFL  LAKR+K N  +YENY RIY+PE  P +  P E+LRVNVLF+HIQ M
Sbjct: 362 PAFGCVLMKDFLSELAKRIKHNNTSYENYHRIYVPEGKPLRDNPNESLRVNVLFQHIQNM 421

Query: 182 LSSNMVVISE-------------------------------AGATLGYAQASLDDRVVLC 241
           LSS   V++E                                GATLGYAQA  + RV+ C
Sbjct: 422 LSSESAVLAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSVGATLGYAQAMPNRRVIAC 481

Query: 242 IGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMH 301
           IGDGSFQ+  QD+STM+R GQ+ IIFLINNGGYTIEVEIHDGPYNVIKNW+YTA V+A+H
Sbjct: 482 IGDGSFQVTAQDVSTMIRCGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTAFVEAIH 541

Query: 302 NHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAAINS 334
           N  G CWTAKV+ EEELVKAI  A  + K+  CFIE IVHKDDTSKELLE+G++++A NS
Sbjct: 542 NGEGKCWTAKVRCEEELVKAINTATNEEKESFCFIEVIVHKDDTSKELLEWGSRVSAANS 601

BLAST of Bhi03G001707 vs. NCBI nr
Match: XP_038882338.1 (pyruvate decarboxylase 1-like, partial [Benincasa hispida])

HSP 1 Score: 655.6 bits (1690), Expect = 2.3e-184
Identity = 333/364 (91.48%), Postives = 333/364 (91.48%), Query Frame = 0

Query: 1   MAVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPH 60
           MAVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPH
Sbjct: 261 MAVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPH 320

Query: 61  FIGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPN 120
           FIGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPN
Sbjct: 321 FIGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPN 380

Query: 121 GPSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQK 180
           GPSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQK
Sbjct: 381 GPSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQK 440

Query: 181 MLSSNMVVISEA-------------------------------GATLGYAQASLDDRVVL 240
           MLSSNMVVISEA                               GATLGYAQASLDDRVVL
Sbjct: 441 MLSSNMVVISEAGDSWFHCQKLELPRSCGYEMQLVYASIGWSLGATLGYAQASLDDRVVL 500

Query: 241 CIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAM 300
           CIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAM
Sbjct: 501 CIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAM 560

Query: 301 HNHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAAIN 334
           HNHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAAIN
Sbjct: 561 HNHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAAIN 620

BLAST of Bhi03G001707 vs. NCBI nr
Match: TYK10923.1 (pyruvate decarboxylase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 597.0 bits (1538), Expect = 9.8e-167
Identity = 302/369 (81.84%), Postives = 317/369 (85.91%), Query Frame = 0

Query: 1   MAVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPH 60
           MAVEKAA+LLN AVKPVLVGGKKLR AKAQA FVELAN C+YAVAVTPSAKGMFPE HPH
Sbjct: 262 MAVEKAAQLLNGAVKPVLVGGKKLRVAKAQAAFVELANTCDYAVAVTPSAKGMFPENHPH 321

Query: 61  FIGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPN 120
           F+GTYWGVLSTAFCGETVEIADASIFVGA+FDELETVGFSLSYKKN AII+ETDHVVFPN
Sbjct: 322 FMGTYWGVLSTAFCGETVEIADASIFVGAIFDELETVGFSLSYKKNKAIIVETDHVVFPN 381

Query: 121 GPSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQK 180
           GP FGPILMKDFLWALAKR+KPN+ AYENYRRIYIPESSPA SEPGEALRVNVLFKHIQK
Sbjct: 382 GPRFGPILMKDFLWALAKRVKPNSTAYENYRRIYIPESSPATSEPGEALRVNVLFKHIQK 441

Query: 181 MLSSNMVVISEA-------------------------------GATLGYAQA--SLDDRV 240
           ML+++M VISEA                               GATLGYAQA  S +DRV
Sbjct: 442 MLTNDMTVISEAGDSWFHTQKLRLPQSCGYEMQIVYASIGWSLGATLGYAQASSSSNDRV 501

Query: 241 VLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVD 300
           VLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDY+ALVD
Sbjct: 502 VLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYSALVD 561

Query: 301 AMHNHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAA 337
           AMHNHNGNCWT KVQTEEELVKAIEIA  D  DCLCFIE IVH+DDTSKELLEFGNKLA 
Sbjct: 562 AMHNHNGNCWTTKVQTEEELVKAIEIATRDHNDCLCFIEVIVHRDDTSKELLEFGNKLAE 621

BLAST of Bhi03G001707 vs. NCBI nr
Match: XP_008464266.1 (PREDICTED: pyruvate decarboxylase 1-like [Cucumis melo] >KAA0037738.1 pyruvate decarboxylase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 597.0 bits (1538), Expect = 9.8e-167
Identity = 302/369 (81.84%), Postives = 317/369 (85.91%), Query Frame = 0

Query: 1   MAVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPH 60
           MAVEKAA+LLN AVKPVLVGGKKLR AKAQA FVELAN C+YAVAVTPSAKGMFPE HPH
Sbjct: 262 MAVEKAAQLLNGAVKPVLVGGKKLRVAKAQAAFVELANTCDYAVAVTPSAKGMFPENHPH 321

Query: 61  FIGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPN 120
           F+GTYWGVLSTAFCGETVEIADASIFVGA+FDELETVGFSLSYKKN AII+ETDHVVFPN
Sbjct: 322 FMGTYWGVLSTAFCGETVEIADASIFVGAIFDELETVGFSLSYKKNKAIIVETDHVVFPN 381

Query: 121 GPSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQK 180
           GP FGPILMKDFLWALAKR+KPN+ AYENYRRIYIPESSPA SEPGEALRVNVLFKHIQK
Sbjct: 382 GPRFGPILMKDFLWALAKRVKPNSTAYENYRRIYIPESSPATSEPGEALRVNVLFKHIQK 441

Query: 181 MLSSNMVVISEA-------------------------------GATLGYAQA--SLDDRV 240
           ML+++M VISEA                               GATLGYAQA  S +DRV
Sbjct: 442 MLTNDMTVISEAGDSWFHTQKLRLPQSCGYEMQIVYASIGWSLGATLGYAQASSSSNDRV 501

Query: 241 VLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVD 300
           VLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDY+ALVD
Sbjct: 502 VLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYSALVD 561

Query: 301 AMHNHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAA 337
           AMHNHNGNCWT KVQTEEELVKAIEIA  D  DCLCFIE IVH+DDTSKELLEFGNKLA 
Sbjct: 562 AMHNHNGNCWTTKVQTEEELVKAIEIATRDHNDCLCFIEVIVHRDDTSKELLEFGNKLAE 621

BLAST of Bhi03G001707 vs. NCBI nr
Match: XP_004148630.1 (pyruvate decarboxylase 1 [Cucumis sativus] >KGN54657.2 hypothetical protein Csa_018093 [Cucumis sativus])

HSP 1 Score: 582.4 bits (1500), Expect = 2.5e-162
Identity = 294/368 (79.89%), Postives = 309/368 (83.97%), Query Frame = 0

Query: 1   MAVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPH 60
           MAVEKAA LLN AVKPVLVGGKKLR AKAQA F+ELAN C+YAVAVTPSAKGMFPE HPH
Sbjct: 261 MAVEKAAELLNGAVKPVLVGGKKLRVAKAQAAFMELANTCDYAVAVTPSAKGMFPENHPH 320

Query: 61  FIGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPN 120
           FIGTYWGV+STAFC ETVEIAD SIF GA+FDELETVGFSLSYKKN AII+ETDHVVFPN
Sbjct: 321 FIGTYWGVISTAFCSETVEIADGSIFAGAIFDELETVGFSLSYKKNKAIIVETDHVVFPN 380

Query: 121 GPSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQK 180
           G  FGPILMKDFLWAL KR+KPN+ +YENYRRIYIPESSPA SEPGE LRVNVLFKHIQK
Sbjct: 381 GLRFGPILMKDFLWALTKRVKPNSTSYENYRRIYIPESSPATSEPGEGLRVNVLFKHIQK 440

Query: 181 MLSSNMVVISEA-------------------------------GATLGYAQASLDD-RVV 240
           ML+ +M VISEA                               GATLGYAQAS  + RVV
Sbjct: 441 MLTDDMAVISEAGDSWFHGQKLKLPQSCGYEMQMVYASIGWSLGATLGYAQASSSNGRVV 500

Query: 241 LCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDA 300
           LCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYN+IKNWDY ALVDA
Sbjct: 501 LCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNIIKNWDYCALVDA 560

Query: 301 MHNHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAAI 337
           MHNHNGNCWT KVQTEE+LVKAIEIA  D  DCLCFIEAIVH+DDTSKELLEFGNKLA +
Sbjct: 561 MHNHNGNCWTRKVQTEEQLVKAIEIATRDHNDCLCFIEAIVHRDDTSKELLEFGNKLAEM 620

BLAST of Bhi03G001707 vs. NCBI nr
Match: XP_022144025.1 (pyruvate decarboxylase 2-like, partial [Momordica charantia])

HSP 1 Score: 574.7 bits (1480), Expect = 5.2e-160
Identity = 290/366 (79.23%), Postives = 308/366 (84.15%), Query Frame = 0

Query: 2   AVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPHF 61
           AVEKAA+LLNSAVKPV V GKKLR AKAQA FVELA+AC YAVAVTPSAKG+FPE HPHF
Sbjct: 121 AVEKAAQLLNSAVKPVAVAGKKLRLAKAQAAFVELADACGYAVAVTPSAKGLFPENHPHF 180

Query: 62  IGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPNG 121
           IGTYWGVLSTAFCGETVEIADAS+F G  FDELETVGFSL YKKNNAII+E + VVFPNG
Sbjct: 181 IGTYWGVLSTAFCGETVEIADASLFAGPTFDELETVGFSLCYKKNNAIIVEPERVVFPNG 240

Query: 122 PSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQKM 181
           PSFGPILM DFL AL KRLKPN  AYENYRRIY+PES P +SEPGEALRVNVLFKHIQKM
Sbjct: 241 PSFGPILMMDFLRALTKRLKPNPTAYENYRRIYVPESGPLQSEPGEALRVNVLFKHIQKM 300

Query: 182 LSSNMVVISEA-------------------------------GATLGYAQASLDDRVVLC 241
           LSSNM VISEA                               GATLGYAQA+ ++R+VLC
Sbjct: 301 LSSNMAVISEAGDSWFHCQKLKLPQSCGYEMQIIYASIGWSLGATLGYAQAASNERMVLC 360

Query: 242 IGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMH 301
           +GDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMH
Sbjct: 361 MGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMH 420

Query: 302 NHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAAINS 337
           N NGNCWT KV+TEEELVKAI+IA G++ DCLCFIE IVH+DDTSKELLEFGNKLAAINS
Sbjct: 421 NANGNCWTTKVKTEEELVKAIQIAMGEKNDCLCFIETIVHRDDTSKELLEFGNKLAAINS 480

BLAST of Bhi03G001707 vs. ExPASy TrEMBL
Match: A0A5D3CG96 (Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold107G00020 PE=3 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 4.7e-167
Identity = 302/369 (81.84%), Postives = 317/369 (85.91%), Query Frame = 0

Query: 1   MAVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPH 60
           MAVEKAA+LLN AVKPVLVGGKKLR AKAQA FVELAN C+YAVAVTPSAKGMFPE HPH
Sbjct: 262 MAVEKAAQLLNGAVKPVLVGGKKLRVAKAQAAFVELANTCDYAVAVTPSAKGMFPENHPH 321

Query: 61  FIGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPN 120
           F+GTYWGVLSTAFCGETVEIADASIFVGA+FDELETVGFSLSYKKN AII+ETDHVVFPN
Sbjct: 322 FMGTYWGVLSTAFCGETVEIADASIFVGAIFDELETVGFSLSYKKNKAIIVETDHVVFPN 381

Query: 121 GPSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQK 180
           GP FGPILMKDFLWALAKR+KPN+ AYENYRRIYIPESSPA SEPGEALRVNVLFKHIQK
Sbjct: 382 GPRFGPILMKDFLWALAKRVKPNSTAYENYRRIYIPESSPATSEPGEALRVNVLFKHIQK 441

Query: 181 MLSSNMVVISEA-------------------------------GATLGYAQA--SLDDRV 240
           ML+++M VISEA                               GATLGYAQA  S +DRV
Sbjct: 442 MLTNDMTVISEAGDSWFHTQKLRLPQSCGYEMQIVYASIGWSLGATLGYAQASSSSNDRV 501

Query: 241 VLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVD 300
           VLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDY+ALVD
Sbjct: 502 VLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYSALVD 561

Query: 301 AMHNHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAA 337
           AMHNHNGNCWT KVQTEEELVKAIEIA  D  DCLCFIE IVH+DDTSKELLEFGNKLA 
Sbjct: 562 AMHNHNGNCWTTKVQTEEELVKAIEIATRDHNDCLCFIEVIVHRDDTSKELLEFGNKLAE 621

BLAST of Bhi03G001707 vs. ExPASy TrEMBL
Match: A0A5A7T4K4 (Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold141G00340 PE=3 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 4.7e-167
Identity = 302/369 (81.84%), Postives = 317/369 (85.91%), Query Frame = 0

Query: 1   MAVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPH 60
           MAVEKAA+LLN AVKPVLVGGKKLR AKAQA FVELAN C+YAVAVTPSAKGMFPE HPH
Sbjct: 262 MAVEKAAQLLNGAVKPVLVGGKKLRVAKAQAAFVELANTCDYAVAVTPSAKGMFPENHPH 321

Query: 61  FIGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPN 120
           F+GTYWGVLSTAFCGETVEIADASIFVGA+FDELETVGFSLSYKKN AII+ETDHVVFPN
Sbjct: 322 FMGTYWGVLSTAFCGETVEIADASIFVGAIFDELETVGFSLSYKKNKAIIVETDHVVFPN 381

Query: 121 GPSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQK 180
           GP FGPILMKDFLWALAKR+KPN+ AYENYRRIYIPESSPA SEPGEALRVNVLFKHIQK
Sbjct: 382 GPRFGPILMKDFLWALAKRVKPNSTAYENYRRIYIPESSPATSEPGEALRVNVLFKHIQK 441

Query: 181 MLSSNMVVISEA-------------------------------GATLGYAQA--SLDDRV 240
           ML+++M VISEA                               GATLGYAQA  S +DRV
Sbjct: 442 MLTNDMTVISEAGDSWFHTQKLRLPQSCGYEMQIVYASIGWSLGATLGYAQASSSSNDRV 501

Query: 241 VLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVD 300
           VLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDY+ALVD
Sbjct: 502 VLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYSALVD 561

Query: 301 AMHNHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAA 337
           AMHNHNGNCWT KVQTEEELVKAIEIA  D  DCLCFIE IVH+DDTSKELLEFGNKLA 
Sbjct: 562 AMHNHNGNCWTTKVQTEEELVKAIEIATRDHNDCLCFIEVIVHRDDTSKELLEFGNKLAE 621

BLAST of Bhi03G001707 vs. ExPASy TrEMBL
Match: A0A1S3CL30 (Pyruvate decarboxylase OS=Cucumis melo OX=3656 GN=LOC103502191 PE=3 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 4.7e-167
Identity = 302/369 (81.84%), Postives = 317/369 (85.91%), Query Frame = 0

Query: 1   MAVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPH 60
           MAVEKAA+LLN AVKPVLVGGKKLR AKAQA FVELAN C+YAVAVTPSAKGMFPE HPH
Sbjct: 262 MAVEKAAQLLNGAVKPVLVGGKKLRVAKAQAAFVELANTCDYAVAVTPSAKGMFPENHPH 321

Query: 61  FIGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPN 120
           F+GTYWGVLSTAFCGETVEIADASIFVGA+FDELETVGFSLSYKKN AII+ETDHVVFPN
Sbjct: 322 FMGTYWGVLSTAFCGETVEIADASIFVGAIFDELETVGFSLSYKKNKAIIVETDHVVFPN 381

Query: 121 GPSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQK 180
           GP FGPILMKDFLWALAKR+KPN+ AYENYRRIYIPESSPA SEPGEALRVNVLFKHIQK
Sbjct: 382 GPRFGPILMKDFLWALAKRVKPNSTAYENYRRIYIPESSPATSEPGEALRVNVLFKHIQK 441

Query: 181 MLSSNMVVISEA-------------------------------GATLGYAQA--SLDDRV 240
           ML+++M VISEA                               GATLGYAQA  S +DRV
Sbjct: 442 MLTNDMTVISEAGDSWFHTQKLRLPQSCGYEMQIVYASIGWSLGATLGYAQASSSSNDRV 501

Query: 241 VLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVD 300
           VLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDY+ALVD
Sbjct: 502 VLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYSALVD 561

Query: 301 AMHNHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAA 337
           AMHNHNGNCWT KVQTEEELVKAIEIA  D  DCLCFIE IVH+DDTSKELLEFGNKLA 
Sbjct: 562 AMHNHNGNCWTTKVQTEEELVKAIEIATRDHNDCLCFIEVIVHRDDTSKELLEFGNKLAE 621

BLAST of Bhi03G001707 vs. ExPASy TrEMBL
Match: A0A0A0KZZ6 (Pyruvate decarboxylase OS=Cucumis sativus OX=3659 GN=Csa_4G312270 PE=3 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.2e-162
Identity = 294/368 (79.89%), Postives = 309/368 (83.97%), Query Frame = 0

Query: 1   MAVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPH 60
           MAVEKAA LLN AVKPVLVGGKKLR AKAQA F+ELAN C+YAVAVTPSAKGMFPE HPH
Sbjct: 1   MAVEKAAELLNGAVKPVLVGGKKLRVAKAQAAFMELANTCDYAVAVTPSAKGMFPENHPH 60

Query: 61  FIGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPN 120
           FIGTYWGV+STAFC ETVEIAD SIF GA+FDELETVGFSLSYKKN AII+ETDHVVFPN
Sbjct: 61  FIGTYWGVISTAFCSETVEIADGSIFAGAIFDELETVGFSLSYKKNKAIIVETDHVVFPN 120

Query: 121 GPSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQK 180
           G  FGPILMKDFLWAL KR+KPN+ +YENYRRIYIPESSPA SEPGE LRVNVLFKHIQK
Sbjct: 121 GLRFGPILMKDFLWALTKRVKPNSTSYENYRRIYIPESSPATSEPGEGLRVNVLFKHIQK 180

Query: 181 MLSSNMVVISEA-------------------------------GATLGYAQASLDD-RVV 240
           ML+ +M VISEA                               GATLGYAQAS  + RVV
Sbjct: 181 MLTDDMAVISEAGDSWFHGQKLKLPQSCGYEMQMVYASIGWSLGATLGYAQASSSNGRVV 240

Query: 241 LCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDA 300
           LCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYN+IKNWDY ALVDA
Sbjct: 241 LCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNIIKNWDYCALVDA 300

Query: 301 MHNHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAAI 337
           MHNHNGNCWT KVQTEE+LVKAIEIA  D  DCLCFIEAIVH+DDTSKELLEFGNKLA +
Sbjct: 301 MHNHNGNCWTRKVQTEEQLVKAIEIATRDHNDCLCFIEAIVHRDDTSKELLEFGNKLAEM 360

BLAST of Bhi03G001707 vs. ExPASy TrEMBL
Match: A0A6J1CQH4 (Pyruvate decarboxylase OS=Momordica charantia OX=3673 GN=LOC111013817 PE=3 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 2.5e-160
Identity = 290/366 (79.23%), Postives = 308/366 (84.15%), Query Frame = 0

Query: 2   AVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANACEYAVAVTPSAKGMFPEQHPHF 61
           AVEKAA+LLNSAVKPV V GKKLR AKAQA FVELA+AC YAVAVTPSAKG+FPE HPHF
Sbjct: 121 AVEKAAQLLNSAVKPVAVAGKKLRLAKAQAAFVELADACGYAVAVTPSAKGLFPENHPHF 180

Query: 62  IGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFSLSYKKNNAIIIETDHVVFPNG 121
           IGTYWGVLSTAFCGETVEIADAS+F G  FDELETVGFSL YKKNNAII+E + VVFPNG
Sbjct: 181 IGTYWGVLSTAFCGETVEIADASLFAGPTFDELETVGFSLCYKKNNAIIVEPERVVFPNG 240

Query: 122 PSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSPAKSEPGEALRVNVLFKHIQKM 181
           PSFGPILM DFL AL KRLKPN  AYENYRRIY+PES P +SEPGEALRVNVLFKHIQKM
Sbjct: 241 PSFGPILMMDFLRALTKRLKPNPTAYENYRRIYVPESGPLQSEPGEALRVNVLFKHIQKM 300

Query: 182 LSSNMVVISEA-------------------------------GATLGYAQASLDDRVVLC 241
           LSSNM VISEA                               GATLGYAQA+ ++R+VLC
Sbjct: 301 LSSNMAVISEAGDSWFHCQKLKLPQSCGYEMQIIYASIGWSLGATLGYAQAASNERMVLC 360

Query: 242 IGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMH 301
           +GDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMH
Sbjct: 361 MGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMH 420

Query: 302 NHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIVHKDDTSKELLEFGNKLAAINS 337
           N NGNCWT KV+TEEELVKAI+IA G++ DCLCFIE IVH+DDTSKELLEFGNKLAAINS
Sbjct: 421 NANGNCWTTKVKTEEELVKAIQIAMGEKNDCLCFIETIVHRDDTSKELLEFGNKLAAINS 480

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G54960.11.3e-12160.06pyruvate decarboxylase-2 [more]
AT4G33070.11.9e-12060.06Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
AT5G01320.13.6e-11959.23Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
AT5G01330.13.1e-11858.95pyruvate decarboxylase-3 [more]
AT5G17380.15.1e-0421.80Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
Match NameE-valueIdentityDescription
P518514.0e-12362.81Pyruvate decarboxylase 2 (Fragment) OS=Pisum sativum OX=3888 GN=PDC2 PE=2 SV=1[more]
A2Y5L94.5e-12259.78Pyruvate decarboxylase 1 OS=Oryza sativa subsp. indica OX=39946 GN=PDC1 PE=2 SV=... [more]
Q0DHF61.3e-12159.78Pyruvate decarboxylase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PDC1 PE=2 S... [more]
A2XFI34.9e-12158.95Pyruvate decarboxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=PDC2 PE=2 SV=... [more]
Q9FFT41.9e-12060.06Pyruvate decarboxylase 2 OS=Arabidopsis thaliana OX=3702 GN=PDC2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038882338.12.3e-18491.48pyruvate decarboxylase 1-like, partial [Benincasa hispida][more]
TYK10923.19.8e-16781.84pyruvate decarboxylase 1-like [Cucumis melo var. makuwa][more]
XP_008464266.19.8e-16781.84PREDICTED: pyruvate decarboxylase 1-like [Cucumis melo] >KAA0037738.1 pyruvate d... [more]
XP_004148630.12.5e-16279.89pyruvate decarboxylase 1 [Cucumis sativus] >KGN54657.2 hypothetical protein Csa_... [more]
XP_022144025.15.2e-16079.23pyruvate decarboxylase 2-like, partial [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A5D3CG964.7e-16781.84Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1... [more]
A0A5A7T4K44.7e-16781.84Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1... [more]
A0A1S3CL304.7e-16781.84Pyruvate decarboxylase OS=Cucumis melo OX=3656 GN=LOC103502191 PE=3 SV=1[more]
A0A0A0KZZ61.2e-16279.89Pyruvate decarboxylase OS=Cucumis sativus OX=3659 GN=Csa_4G312270 PE=3 SV=1[more]
A0A6J1CQH42.5e-16079.23Pyruvate decarboxylase OS=Momordica charantia OX=3673 GN=LOC111013817 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011766Thiamine pyrophosphate enzyme, C-terminal TPP-bindingPFAMPF02775TPP_enzyme_Ccoord: 192..303
e-value: 2.7E-11
score: 43.5
NoneNo IPR availableGENE3D3.40.50.1220coord: 1..155
e-value: 4.2E-37
score: 129.3
NoneNo IPR availableGENE3D3.40.50.970coord: 164..318
e-value: 8.8E-36
score: 125.2
NoneNo IPR availablePANTHERPTHR43452:SF25PYRUVATE DECARBOXYLASE 1coord: 193..334
NoneNo IPR availablePANTHERPTHR43452:SF25PYRUVATE DECARBOXYLASE 1coord: 2..194
IPR012000Thiamine pyrophosphate enzyme, central domainPFAMPF00205TPP_enzyme_Mcoord: 3..114
e-value: 2.7E-21
score: 75.8
IPR012110Thiamine pyrophosphate (TPP)-dependent enzymePANTHERPTHR43452PYRUVATE DECARBOXYLASEcoord: 193..334
IPR012110Thiamine pyrophosphate (TPP)-dependent enzymePANTHERPTHR43452PYRUVATE DECARBOXYLASEcoord: 2..194
IPR029035DHS-like NAD/FAD-binding domain superfamilySUPERFAMILY52467DHS-like NAD/FAD-binding domaincoord: 2..144
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 170..332

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M001707Bhi03M001707mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0004737 pyruvate decarboxylase activity
molecular_function GO:0030976 thiamine pyrophosphate binding
molecular_function GO:0016831 carboxy-lyase activity
molecular_function GO:0003824 catalytic activity