Bhi03G001252 (gene) Wax gourd (B227) v1

Overview
NameBhi03G001252
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionHMA domain-containing protein
Locationchr3: 34271530 .. 34275776 (-)
RNA-Seq ExpressionBhi03G001252
SyntenyBhi03G001252
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGTGTGTTATCACATCCTTAAAAAAACAGGCCTCATTATTCTCTCTCTCAACCACACAAAGAGAAGTTCACACTTTTTGAGCCGAGAGAGAAAAATTAGAAAGAAAAATACCAAAAACCATTTTATCCAAAAACACACAAAATCTCATCATCATATCATGAAAACCATCGATTTCTTCTGCGCCTCCCAAGCCTCCACCGCCGTCGACCAACCTTCATCTTCCCCCGCCGCTGCCGCCTCCGGAAGAGCCATCGACCGCCACAACCCCATCATCGCCGACGGCAGAAGATCAAACTTCACTTCTAGAACTACTTTCCCCAACCCTCCGTGTTCCTCCCAATACTCCCCCATAAATCCTCTCCCTTACCACCAACTCCACGCCGCCGCCGCCTCCCCAAATGTCGCCGCCGACCAGATCCGCTCCGGCGCCTCTGCAACTCATAAGGGTTTGAAGATGAAGAAGAAGAAAAAAAATTCATCTATTATTACAACCGACTTTGTAAGGTTTGTTCATTTCCTTCAAACTTATCCATTCACTTTTAACTCAAATCTCATTTATTTATATTAATTATCATTGCTTTTTTTTTTTTTTTTTGAAAATTTACAATTTGTTTACACTCATAATTGATATTTTCTTTAATGTACTTTCTAGATTATTTCATGCTATTAAATTCATTTTCGTGATATATCAATATTATCGATTTTATTTATTATTAAAACATTTTTTTTTTTTATTATTGAAACATTGACATTTTAAATTTTAGATCATTTTAGTTCTAGTTAAAAATAGTTTTCTAGCAATATTTTAAGTGCATTCAATATTCGAATAAAAAAATTAAATGTGTTTTTTTAAAAAAATATTTATATCACACAAAAAATTTAAATTAAACATGCTATTTTTAAATATATGAAAATGAGTTTAAGTTATTTATAGATATAGTAAAATATCATTGTCTAAAATTGATAGATATATATTTTATTATATTTAAAGTATTTTAGATCGTTTTACCATTTAAAATAATAAATATAATTAAATAATTAATTAGATGCACTCAAAATGCATCTCAGTATTTTTTATAATTAATATGAGTTTTAATATGCACTAAATTCAAAGTTTTTTATTAAAAAATAAAAGCATTTAATAGAAATGGTGTGGGGAAATTTAATTTATTAATTGTAAAGAATAGAAAAATAATGATTTAGTTTGACCACTTAGGTGGAGTTGTGCGAAGCCCAGTGATTTGGCCACGCCTCCTGGGTCGATGCGATACCTTTTGAACGACAAGTCGGTTCGTGATGGTTCGATGGATCGTCTTTCCACATCCATTCAACTTAACAAAAAGGTTAATTCACCAAATGAAATTCCACAACTATCTAAACCAACGGCCGAGATTTCTTTGGAGGACGACTGCAAAAAATCTCCACCTTCCAATCAGGTTAGTATTTTTCACCCTATTCAAGGGAAAAAAGAAAATCAATTGCAAGCAAATAGGCCGTTCGACTTTCAAATTTGTGTTAAATATAGCTCCCTCTAAAAGTAACTAATAAATATGAAATTAAAATTTTAAAATTTATTCAACATAAGATTTAATCATTAATTTTATCTGATTGGTCAATTATTAATATTTTTTTAAAAAAGAATATATTAGAGATCTATTAAATACAAAATTTAAGGTTTGGGGACTTTCTACGTAAAATTGAAAGTTAAGAAATTTATTAGATACTTTTAAAAATTTAGAAATCACCTAAGAGACATAAACTTACAAGTTTAGAGACTAAGTTTATAATTTAAAAATTTTCTATTCCTCTCTCTTATTTATCAAAAACCTAGAGTAGTCCACACGATTACCTAAGCAAACTTTTGCAATAGGTTAAAATATCATTTTGGTCTCTGTACTTTGAAATTTGTTCAATTTGATGTATTTCCAAATATCCATTTTAGTTCATGTACTTTAAAATAAATCTAAATTTAGACATTCGAAGTTATTTTTTTTGTTGAAATTGATTAAATAATCATAATAATTTTTATTCAAAGAAATACGACATGTGACTATGTTTTCAAAATTTATAGTGAAAGTTCTAATAAAAATAAAAAGTAATTAGAAAATCCTCAATAAACTAGTAGTATGAACTAAATTTAAGATTTATTAAAAGTAAATGAACTAAAATTAGACATTTGAAATAAAGACTAAACTTAAAACAAACGGTAAAATATAGGCACCAAAATGATAACTTTAACTTAAAAATAATTATAATGCCATGAATGAGATGTTTTTAAATATAAGAAAAATGAATCAAAATATTTACAAATATAGTAAAATTTCATTGTTTATCTACAATAGACCGTGATAGACTATTATCTGTATTATGATAAATACATATAGTAGTCTATCGCAGTCTATCACATGTGATATTTTGTTATTATCTGTAAATATTTTCAATAATTTTGTAATTTAAAATAATTTTTCATGATTTTGAAATAGAATTGTGTAAAAGAAATTTGTAATTTTATTTTATTTTATTTATTTATTTATTTATTATTTATTTTTAATTTTTGTATGTATGTGTTTAATTAAGGTGATCTCTCACTCTCAAGTTGTGGTTTTGAGGGTATCATTGCATTGCAGAGGCTGTGAAGGAAAACTGAGGAAACATCTTTCCAAAATGGAAGGTAAGCAATGAAAAAGGTTTAAATATAAAGCTAAGCTATTCCCTTTTTCTTTTTAAGTAAATTTATTATTGATTTTTTTTTTTTTAACTAGATGATTAGAAAAATTATATGTTACAACCCGACTTAGAATCTTCAAAAGATTTGAAAATTAAGTTACACTACTTCCATCTCCAAATTTCTTTATTTGTTATCTACTTTTTACTGATAGTTTAAAAAATAAAGCCAAAATTTGAAAAACTAAAAATGTAAATTTTAAACAACTTGATTTTGTTTTTTAAATTTGACTATAAATTAAACTATTGTATTTTAGAAAAAATGCAAATCAAAATTGGGAGGAAATATGCTTAATTTTTTAAAAATAAAAAACCAAAAACAAAATGATTACCAAACGGAGTTTGGTCACATTTGATAACCATTTCATTTAGTTTTTTATTTTTGAAAACTAAATCTATAGACATTACTTTCTTCTCCAAATTTCTTCTTTTGTTATTTACTTTTTATCAATGGTTTAAAAAACCAAGCAAAAATATAAAAACTAAGTATCGTTTGGTAATCATTTTGTTCTTAATTTTTTATTTTTGAAAATTAAACCCCCAATTTCTTACAATGATTTAGATTTTCTTAAATACAATGGTTGAATTCTCAGTCAAATTCTAAAAACAATAACAACTTTTAAAAGTTACTTTTTTTAGTTTTCAAATTTTAATTTGTTTTTTAAATCATTGATAAAAAGTAGATAACAAATGAAGAAATTTAGAGGTCGAAATACCAAAGAATTATCAAACGAGACCTAAAAAGTAGTTTTAAAAAATTTGTTTTTGGTTTTTGAATTTGGTCAATAACTCAACCAACGTACTTAAGAAAGTTACAAATCCCGATAGGAATTGGAAAACAAATTTATATAGTGACTCTTTTGTTCTTTAATCTTTTAGATAGAATCTCATACATTTTAATCGAGAAAACACACTTTTATAATAATTGAACTATTCTCGGTTTTACTTATGATTGGTTTGAAAATGCAGGAGTGAACTCATTCAATATTGATTTTGCAGCCAAAAAGGTGACAATAATGGGAAATATAACACCAGAGGGTATGTTGGAAAGTGTATCAAAGGTCAAAAATGCCCAATTTTGGCCATATGCAGACCCAACACCAACACCAAACCCTAATCCTATTCATCAACAAAATGTATTGAAGAAAGCCTGAACAATTTCATCTCCTACTTTCTTTTTTTTCCTTCTAAATAATTGAGCTTATGTTTATTGTTTAATAATGTAATTGCTAGAAATGCTTCGACACTCCCAGTTATAAGTTGAAAAAATTAAAGCTCCGTTTGATAACTATTTTATTTTTTAAAATTAAATTTAGATATTACTTTTATCTCTAAATTTCTTCATTTGTTATTTACTTTTCATCGGTGGTTTAAAAAACCAAACCAAATTTAGATAACTAAAAAAGTAATTTTCAAAAAGTTGTTTTTTCTTTTTGGAATTTAGCTAAGAATTCAACCATTGTGATGTAGATTATTGTAAGAAATGTGAATGATGTAGGCTTAATTTTCAAAAACAAAAATT

mRNA sequence

GTGTGTGTTATCACATCCTTAAAAAAACAGGCCTCATTATTCTCTCTCTCAACCACACAAAGAGAAGTTCACACTTTTTGAGCCGAGAGAGAAAAATTAGAAAGAAAAATACCAAAAACCATTTTATCCAAAAACACACAAAATCTCATCATCATATCATGAAAACCATCGATTTCTTCTGCGCCTCCCAAGCCTCCACCGCCGTCGACCAACCTTCATCTTCCCCCGCCGCTGCCGCCTCCGGAAGAGCCATCGACCGCCACAACCCCATCATCGCCGACGGCAGAAGATCAAACTTCACTTCTAGAACTACTTTCCCCAACCCTCCGTGTTCCTCCCAATACTCCCCCATAAATCCTCTCCCTTACCACCAACTCCACGCCGCCGCCGCCTCCCCAAATGTCGCCGCCGACCAGATCCGCTCCGGCGCCTCTGCAACTCATAAGGGTTTGAAGATGAAGAAGAAGAAAAAAAATTCATCTATTATTACAACCGACTTTGTAAGGTGGAGTTGTGCGAAGCCCAGTGATTTGGCCACGCCTCCTGGGTCGATGCGATACCTTTTGAACGACAAGTCGGTTCGTGATGGTTCGATGGATCGTCTTTCCACATCCATTCAACTTAACAAAAAGGTTAATTCACCAAATGAAATTCCACAACTATCTAAACCAACGGCCGAGATTTCTTTGGAGGACGACTGCAAAAAATCTCCACCTTCCAATCAGGTGATCTCTCACTCTCAAGTTGTGGTTTTGAGGGTATCATTGCATTGCAGAGGCTGTGAAGGAAAACTGAGGAAACATCTTTCCAAAATGGAAGGAGTGAACTCATTCAATATTGATTTTGCAGCCAAAAAGGTGACAATAATGGGAAATATAACACCAGAGGGTATGTTGGAAAGTGTATCAAAGGTCAAAAATGCCCAATTTTGGCCATATGCAGACCCAACACCAACACCAAACCCTAATCCTATTCATCAACAAAATGTATTGAAGAAAGCCTGAACAATTTCATCTCCTACTTTCTTTTTTTTCCTTCTAAATAATTGAGCTTATGTTTATTGTTTAATAATGTAATTGCTAGAAATGCTTCGACACTCCCAGTTATAAGTTGAAAAAATTAAAGCTCCGTTTGATAACTATTTTATTTTTTAAAATTAAATTTAGATATTACTTTTATCTCTAAATTTCTTCATTTGTTATTTACTTTTCATCGGTGGTTTAAAAAACCAAACCAAATTTAGATAACTAAAAAAGTAATTTTCAAAAAGTTGTTTTTTCTTTTTGGAATTTAGCTAAGAATTCAACCATTGTGATGTAGATTATTGTAAGAAATGTGAATGATGTAGGCTTAATTTTCAAAAACAAAAATT

Coding sequence (CDS)

ATGAAAACCATCGATTTCTTCTGCGCCTCCCAAGCCTCCACCGCCGTCGACCAACCTTCATCTTCCCCCGCCGCTGCCGCCTCCGGAAGAGCCATCGACCGCCACAACCCCATCATCGCCGACGGCAGAAGATCAAACTTCACTTCTAGAACTACTTTCCCCAACCCTCCGTGTTCCTCCCAATACTCCCCCATAAATCCTCTCCCTTACCACCAACTCCACGCCGCCGCCGCCTCCCCAAATGTCGCCGCCGACCAGATCCGCTCCGGCGCCTCTGCAACTCATAAGGGTTTGAAGATGAAGAAGAAGAAAAAAAATTCATCTATTATTACAACCGACTTTGTAAGGTGGAGTTGTGCGAAGCCCAGTGATTTGGCCACGCCTCCTGGGTCGATGCGATACCTTTTGAACGACAAGTCGGTTCGTGATGGTTCGATGGATCGTCTTTCCACATCCATTCAACTTAACAAAAAGGTTAATTCACCAAATGAAATTCCACAACTATCTAAACCAACGGCCGAGATTTCTTTGGAGGACGACTGCAAAAAATCTCCACCTTCCAATCAGGTGATCTCTCACTCTCAAGTTGTGGTTTTGAGGGTATCATTGCATTGCAGAGGCTGTGAAGGAAAACTGAGGAAACATCTTTCCAAAATGGAAGGAGTGAACTCATTCAATATTGATTTTGCAGCCAAAAAGGTGACAATAATGGGAAATATAACACCAGAGGGTATGTTGGAAAGTGTATCAAAGGTCAAAAATGCCCAATTTTGGCCATATGCAGACCCAACACCAACACCAAACCCTAATCCTATTCATCAACAAAATGTATTGAAGAAAGCCTGA

Protein sequence

MKTIDFFCASQASTAVDQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPCSSQYSPINPLPYHQLHAAAASPNVAADQIRSGASATHKGLKMKKKKKNSSIITTDFVRWSCAKPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSIQLNKKVNSPNEIPQLSKPTAEISLEDDCKKSPPSNQVISHSQVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKVTIMGNITPEGMLESVSKVKNAQFWPYADPTPTPNPNPIHQQNVLKKA
Homology
BLAST of Bhi03G001252 vs. TAIR 10
Match: AT3G53530.1 (Chloroplast-targeted copper chaperone protein )

HSP 1 Score: 144.1 bits (362), Expect = 1.8e-34
Identity = 117/276 (42.39%), Postives = 147/276 (53.26%), Query Frame = 0

Query: 1   MKTIDFFCASQASTAVDQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPCSS 60
           MK   F+CASQASTA    +++     + RAIDRHNPII DGRRS FT+       PCSS
Sbjct: 1   MKAGMFYCASQASTA----TANGERTVTARAIDRHNPIIKDGRRS-FTA-------PCSS 60

Query: 61  QYSPINPLPYHQLHAAAASPNVAAD----QIRSGASATHKGLKMKKKKKNSSIITTDFVR 120
               +   PY QL      P+ + D    Q+  G   ++ G  MK    + S+    F  
Sbjct: 61  GDDYV--APYRQLSKITRVPSSSGDGKSVQVDKG-RRSNSGSLMKLISYDVSLARKSF-- 120

Query: 121 WSCAKPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSIQLNKKVNSPNEIPQLSKPTAEIS 180
             C   +   TPPGS RYLL    V         T      + ++P      S P     
Sbjct: 121 -GCVVATP-KTPPGSTRYLLGSDPVSLAGSTGQDTVATEESEASAPKR--GSSGPV---- 180

Query: 181 LEDDCKKSPPSNQVISHSQVVVLRVSL--HCRGCEGKLRKHLSKMEGVNSFNIDFAAKKV 240
             ++ KKS  S       QVVVLRVSL  HCRGC+GK++KHLSKM+GV SFNIDFA+KKV
Sbjct: 181 --EEKKKSSGSGS----DQVVVLRVSLHCHCRGCQGKVKKHLSKMQGVTSFNIDFASKKV 240

Query: 241 TIMGNITPEGMLESVSKVKNAQFWPYADPT-PTPNP 270
           T+ G+ITP  +L  +SKVKNAQFW    P+ P  NP
Sbjct: 241 TVTGDITPLEVLGCLSKVKNAQFWTPPPPSIPRANP 245

BLAST of Bhi03G001252 vs. TAIR 10
Match: AT3G53530.2 (Chloroplast-targeted copper chaperone protein )

HSP 1 Score: 139.4 bits (350), Expect = 4.3e-33
Identity = 117/277 (42.24%), Postives = 147/277 (53.07%), Query Frame = 0

Query: 1   MKTIDFFCASQASTAVDQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPCSS 60
           MK   F+CASQASTA    +++     + RAIDRHNPII DGRRS FT+       PCSS
Sbjct: 1   MKAGMFYCASQASTA----TANGERTVTARAIDRHNPIIKDGRRS-FTA-------PCSS 60

Query: 61  QYSPINPLPYHQLHAAAASPNVAAD----QIRSGASATHKGLKMKKKKKNSSIITTDFVR 120
               +   PY QL      P+ + D    Q+  G   ++ G  MK    + S+    F  
Sbjct: 61  GDDYV--APYRQLSKITRVPSSSGDGKSVQVDKG-RRSNSGSLMKLISYDVSLARKSF-- 120

Query: 121 WSCAKPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSIQLNKKVNSPNEIPQLSKPTAEIS 180
             C   +   TPPGS RYLL    V         T      + ++P      S P     
Sbjct: 121 -GCVVATP-KTPPGSTRYLLGSDPVSLAGSTGQDTVATEESEASAPKR--GSSGPV---- 180

Query: 181 LEDDCKKSPPSNQVISHSQVVVLRVSL--HCRGCEGKLRKHLSKME-GVNSFNIDFAAKK 240
             ++ KKS  S       QVVVLRVSL  HCRGC+GK++KHLSKM+ GV SFNIDFA+KK
Sbjct: 181 --EEKKKSSGSGS----DQVVVLRVSLHCHCRGCQGKVKKHLSKMQVGVTSFNIDFASKK 240

Query: 241 VTIMGNITPEGMLESVSKVKNAQFWPYADPT-PTPNP 270
           VT+ G+ITP  +L  +SKVKNAQFW    P+ P  NP
Sbjct: 241 VTVTGDITPLEVLGCLSKVKNAQFWTPPPPSIPRANP 246

BLAST of Bhi03G001252 vs. TAIR 10
Match: AT2G37390.1 (Chloroplast-targeted copper chaperone protein )

HSP 1 Score: 136.0 bits (341), Expect = 4.8e-32
Identity = 110/282 (39.01%), Postives = 146/282 (51.77%), Query Frame = 0

Query: 7   FCASQASTAV--------DQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPC 66
           FCASQASTA+           ++      SGRAIDRHNPII DGRRS       F   P 
Sbjct: 6   FCASQASTAICSSMDHVHKSTTTDEDERNSGRAIDRHNPIIKDGRRS---FADDFIKLPT 65

Query: 67  SSQYSPINPLPYHQLHAAAASPNVAADQIRSGASATHKGLKMKKKKKNSSIITTDFVRWS 126
           S++   ++     +L       ++   +   G         +K    +  +    F   S
Sbjct: 66  SAEDGEMS---NKKLEIYKGRRSITGRRSTGGGGGGGAAALLKLITNDIGLARKSF---S 125

Query: 127 C-AKPSD--LATPPGSMRYLL-NDKSVRDGSMDRLSTSIQLNKKVNSPNEIPQLSKPTAE 186
           C A+P+   + TP GS RYLL +D     GS+D+             P +  +   P   
Sbjct: 126 CVARPACDLIKTPVGSTRYLLGSDPDSITGSVDQ------------DPAKTVEAEAPAG- 185

Query: 187 ISLEDDCKKSPPSNQVISHSQVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKV 246
              ED       +    +  QVVVL+VSLHCRGCEGK+RKHL++M+GV SFNIDFAAKKV
Sbjct: 186 ---EDKTLTEKKTTCGDTDQQVVVLKVSLHCRGCEGKVRKHLARMQGVTSFNIDFAAKKV 245

Query: 247 TIMGNITPEGMLESVSKVKNAQFWPYADPTPTPNPNPIHQQN 277
           T+ G+ITP  +L+S+SKVKNAQFW   +PT  P PN +  QN
Sbjct: 246 TVTGDITPLEILDSISKVKNAQFW--TNPT-IPKPN-VETQN 258

BLAST of Bhi03G001252 vs. TAIR 10
Match: AT2G37390.2 (Chloroplast-targeted copper chaperone protein )

HSP 1 Score: 135.6 bits (340), Expect = 6.3e-32
Identity = 110/282 (39.01%), Postives = 145/282 (51.42%), Query Frame = 0

Query: 7   FCASQASTAV--------DQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPC 66
           FCASQASTA+           ++      SGRAIDRHNPII DGRRS       F   P 
Sbjct: 6   FCASQASTAICSSMDHVHKSTTTDEDERNSGRAIDRHNPIIKDGRRS---FADDFIKLPT 65

Query: 67  SSQYSPINPLPYHQLHAAAASPNVAADQIRSGASATHKGLKMKKKKKNSSIITTDFVRWS 126
           S++   ++     +L       ++   +   G         +K    +  +    F   S
Sbjct: 66  SAEDGEMS---NKKLEIYKGRRSITGRRSTGGGGGGGAAALLKLITNDIGLARKSF---S 125

Query: 127 C-AKPSD--LATPPGSMRYLL-NDKSVRDGSMDRLSTSIQLNKKVNSPNEIPQLSKPTAE 186
           C A+P+   + TP GS RYLL +D     GS+D+             P +  +   P  E
Sbjct: 126 CVARPACDLIKTPVGSTRYLLGSDPDSITGSVDQ------------DPAKTVEAEAPAGE 185

Query: 187 ISLEDDCKKSPPSNQVISHSQVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKV 246
                D   +          QVVVL+VSLHCRGCEGK+RKHL++M+GV SFNIDFAAKKV
Sbjct: 186 -----DKTLTEKKTTCGDTDQVVVLKVSLHCRGCEGKVRKHLARMQGVTSFNIDFAAKKV 245

Query: 247 TIMGNITPEGMLESVSKVKNAQFWPYADPTPTPNPNPIHQQN 277
           T+ G+ITP  +L+S+SKVKNAQFW   +PT  P PN +  QN
Sbjct: 246 TVTGDITPLEILDSISKVKNAQFW--TNPT-IPKPN-VETQN 257

BLAST of Bhi03G001252 vs. TAIR 10
Match: AT5G02600.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 134.8 bits (338), Expect = 1.1e-31
Identity = 128/335 (38.21%), Postives = 156/335 (46.57%), Query Frame = 0

Query: 8   CASQASTA--------VDQPSSSPAAA--ASGRAIDRHNPIIADGRRSNFTSRTTFPNPP 67
           CASQAST           QPSSS +A     GRAIDRHNPII DGRR           PP
Sbjct: 3   CASQASTTTLCSTMDQTSQPSSSSSATIRLGGRAIDRHNPIIRDGRRL---------TPP 62

Query: 68  CSSQYSPINPLPYHQLHAAAASPNVAADQIRSG-ASATHKGLKMKKKKKNSSIITTDFVR 127
            S   +P          ++++S        R G  S+  K L  +K KK  S +    V 
Sbjct: 63  PSPNLNP---------SSSSSSTYHTPLMTRLGLESSEQKRLAKRKSKKGDSDVGKSPV- 122

Query: 128 WSCAKPSDLATPPGSMRYLLNDKSVRDGSMDR---------------------------- 187
            SC   SD  TP GS RYLL++    DG +D                             
Sbjct: 123 -SCFS-SD--TPQGSSRYLLSNPVFFDGFVDSDPIPIPIDEPEITKADDLNNFHEDRLII 182

Query: 188 -----LSTSIQ-LNKK----------------VNSPNEIPQLSKPTAEI-SLED------ 247
                LSTS   L KK                 ++P   P  + PTA + SLED      
Sbjct: 183 NASKYLSTSASFLEKKQPDFFEGFLDYEPVLSPDNPFSEPTKASPTASLSSLEDKDVSSP 242

Query: 248 DCKKSP---------------PSNQVISHSQVVVLRVSLHCRGCEGKLRKHLSKMEGVNS 260
           D K SP               P  +  S  QVVVLRVSLHC+GC GK++KHLSK++GV S
Sbjct: 243 DFKFSPPPPPPPSPPQSSPPSPPEKNSSSDQVVVLRVSLHCKGCAGKVKKHLSKLKGVTS 302

BLAST of Bhi03G001252 vs. ExPASy Swiss-Prot
Match: Q8RXH8 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 3 OS=Arabidopsis thaliana OX=3702 GN=NAKR3 PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.5e-33
Identity = 117/276 (42.39%), Postives = 147/276 (53.26%), Query Frame = 0

Query: 1   MKTIDFFCASQASTAVDQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPCSS 60
           MK   F+CASQASTA    +++     + RAIDRHNPII DGRRS FT+       PCSS
Sbjct: 1   MKAGMFYCASQASTA----TANGERTVTARAIDRHNPIIKDGRRS-FTA-------PCSS 60

Query: 61  QYSPINPLPYHQLHAAAASPNVAAD----QIRSGASATHKGLKMKKKKKNSSIITTDFVR 120
               +   PY QL      P+ + D    Q+  G   ++ G  MK    + S+    F  
Sbjct: 61  GDDYV--APYRQLSKITRVPSSSGDGKSVQVDKG-RRSNSGSLMKLISYDVSLARKSF-- 120

Query: 121 WSCAKPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSIQLNKKVNSPNEIPQLSKPTAEIS 180
             C   +   TPPGS RYLL    V         T      + ++P      S P     
Sbjct: 121 -GCVVATP-KTPPGSTRYLLGSDPVSLAGSTGQDTVATEESEASAPKR--GSSGPV---- 180

Query: 181 LEDDCKKSPPSNQVISHSQVVVLRVSL--HCRGCEGKLRKHLSKMEGVNSFNIDFAAKKV 240
             ++ KKS  S       QVVVLRVSL  HCRGC+GK++KHLSKM+GV SFNIDFA+KKV
Sbjct: 181 --EEKKKSSGSGS----DQVVVLRVSLHCHCRGCQGKVKKHLSKMQGVTSFNIDFASKKV 240

Query: 241 TIMGNITPEGMLESVSKVKNAQFWPYADPT-PTPNP 270
           T+ G+ITP  +L  +SKVKNAQFW    P+ P  NP
Sbjct: 241 TVTGDITPLEVLGCLSKVKNAQFWTPPPPSIPRANP 245

BLAST of Bhi03G001252 vs. ExPASy Swiss-Prot
Match: Q58FZ0 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 2 OS=Arabidopsis thaliana OX=3702 GN=NAKR2 PE=2 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 6.8e-31
Identity = 110/282 (39.01%), Postives = 146/282 (51.77%), Query Frame = 0

Query: 7   FCASQASTAV--------DQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPC 66
           FCASQASTA+           ++      SGRAIDRHNPII DGRRS       F   P 
Sbjct: 6   FCASQASTAICSSMDHVHKSTTTDEDERNSGRAIDRHNPIIKDGRRS---FADDFIKLPT 65

Query: 67  SSQYSPINPLPYHQLHAAAASPNVAADQIRSGASATHKGLKMKKKKKNSSIITTDFVRWS 126
           S++   ++     +L       ++   +   G         +K    +  +    F   S
Sbjct: 66  SAEDGEMS---NKKLEIYKGRRSITGRRSTGGGGGGGAAALLKLITNDIGLARKSF---S 125

Query: 127 C-AKPSD--LATPPGSMRYLL-NDKSVRDGSMDRLSTSIQLNKKVNSPNEIPQLSKPTAE 186
           C A+P+   + TP GS RYLL +D     GS+D+             P +  +   P   
Sbjct: 126 CVARPACDLIKTPVGSTRYLLGSDPDSITGSVDQ------------DPAKTVEAEAPAG- 185

Query: 187 ISLEDDCKKSPPSNQVISHSQVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKV 246
              ED       +    +  QVVVL+VSLHCRGCEGK+RKHL++M+GV SFNIDFAAKKV
Sbjct: 186 ---EDKTLTEKKTTCGDTDQQVVVLKVSLHCRGCEGKVRKHLARMQGVTSFNIDFAAKKV 245

Query: 247 TIMGNITPEGMLESVSKVKNAQFWPYADPTPTPNPNPIHQQN 277
           T+ G+ITP  +L+S+SKVKNAQFW   +PT  P PN +  QN
Sbjct: 246 TVTGDITPLEILDSISKVKNAQFW--TNPT-IPKPN-VETQN 258

BLAST of Bhi03G001252 vs. ExPASy Swiss-Prot
Match: Q8LDS4 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=NAKR1 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.5e-30
Identity = 128/335 (38.21%), Postives = 156/335 (46.57%), Query Frame = 0

Query: 8   CASQASTA--------VDQPSSSPAAA--ASGRAIDRHNPIIADGRRSNFTSRTTFPNPP 67
           CASQAST           QPSSS +A     GRAIDRHNPII DGRR           PP
Sbjct: 3   CASQASTTTLCSTMDQTSQPSSSSSATIRLGGRAIDRHNPIIRDGRRL---------TPP 62

Query: 68  CSSQYSPINPLPYHQLHAAAASPNVAADQIRSG-ASATHKGLKMKKKKKNSSIITTDFVR 127
            S   +P          ++++S        R G  S+  K L  +K KK  S +    V 
Sbjct: 63  PSPNLNP---------SSSSSSTYHTPLMTRLGLESSEQKRLAKRKSKKGDSDVGKSPV- 122

Query: 128 WSCAKPSDLATPPGSMRYLLNDKSVRDGSMDR---------------------------- 187
            SC   SD  TP GS RYLL++    DG +D                             
Sbjct: 123 -SCFS-SD--TPQGSSRYLLSNPVFFDGFVDSDPIPIPIDEPEITKADDLNNFHEDRLII 182

Query: 188 -----LSTSIQ-LNKK----------------VNSPNEIPQLSKPTAEI-SLED------ 247
                LSTS   L KK                 ++P   P  + PTA + SLED      
Sbjct: 183 NASKYLSTSASFLEKKQPDFFEGFLDYEPVLSPDNPFSEPTKASPTASLSSLEDKDVSSP 242

Query: 248 DCKKSP---------------PSNQVISHSQVVVLRVSLHCRGCEGKLRKHLSKMEGVNS 260
           D K SP               P  +  S  QVVVLRVSLHC+GC GK++KHLSK++GV S
Sbjct: 243 DFKFSPPPPPPPSPPQSSPPSPPEKNSSSDQVVVLRVSLHCKGCAGKVKKHLSKLKGVTS 302

BLAST of Bhi03G001252 vs. ExPASy Swiss-Prot
Match: Q94BT9 (Copper transport protein ATX1 OS=Arabidopsis thaliana OX=3702 GN=ATX1 PE=1 SV=2)

HSP 1 Score: 73.6 bits (179), Expect = 4.1e-12
Identity = 32/66 (48.48%), Postives = 47/66 (71.21%), Query Frame = 0

Query: 194 SQVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKVTIMGNITPEGMLESVSKV- 253
           SQ VVLRV++ C GC G +++ L KMEGV SF++D   +KVT+ GN+ P+ +L++V+K  
Sbjct: 2   SQTVVLRVAMTCEGCVGAVKRVLGKMEGVESFDVDIKEQKVTVKGNVQPDAVLQTVTKTG 61

Query: 254 KNAQFW 259
           K   FW
Sbjct: 62  KKTAFW 67

BLAST of Bhi03G001252 vs. ExPASy Swiss-Prot
Match: O82089 (Copper transport protein CCH OS=Arabidopsis thaliana OX=3702 GN=CCH PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 5.4e-12
Identity = 35/80 (43.75%), Postives = 51/80 (63.75%), Query Frame = 0

Query: 194 SQVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKVTIMGNITPEGMLESVSKV- 253
           +Q VVL+V + C+GC G + + L KMEGV SF+ID   +KVT+ GN+ PE + ++VSK  
Sbjct: 2   AQTVVLKVGMSCQGCVGAVNRVLGKMEGVESFDIDIKEQKVTVKGNVEPEAVFQTVSKTG 61

Query: 254 KNAQFWPY-ADPTPTPNPNP 272
           K   +WP  A+  P    +P
Sbjct: 62  KKTSYWPVEAEAEPKAEADP 81

BLAST of Bhi03G001252 vs. NCBI nr
Match: XP_038881230.1 (protein SODIUM POTASSIUM ROOT DEFECTIVE 3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 558.5 bits (1438), Expect = 3.2e-155
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 0

Query: 1   MKTIDFFCASQASTAVDQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPCSS 60
           MKTIDFFCASQASTAVDQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPCSS
Sbjct: 1   MKTIDFFCASQASTAVDQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPCSS 60

Query: 61  QYSPINPLPYHQLHAAAASPNVAADQIRSGASATHKGLKMKKKKKNSSIITTDFVRWSCA 120
           QYSPINPLPYHQLHAAAASPNVAADQIRSGASATHKGLKMKKKKKNSSIITTDFVRWSCA
Sbjct: 61  QYSPINPLPYHQLHAAAASPNVAADQIRSGASATHKGLKMKKKKKNSSIITTDFVRWSCA 120

Query: 121 KPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSIQLNKKVNSPNEIPQLSKPTAEISLEDD 180
           KPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSIQLNKKVNSPNEIPQLSKPTAEISLEDD
Sbjct: 121 KPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSIQLNKKVNSPNEIPQLSKPTAEISLEDD 180

Query: 181 CKKSPPSNQVISHSQVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKVTIMGNI 240
           CKKSPPSNQVISHSQVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKVTIMGNI
Sbjct: 181 CKKSPPSNQVISHSQVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKVTIMGNI 240

Query: 241 TPEGMLESVSKVKNAQFWPYADPTPTPNPNPIHQQNVLKKA 282
           TPEGMLESVSKVKNAQFWPYADPTPTPNPNPIHQQNVLKKA
Sbjct: 241 TPEGMLESVSKVKNAQFWPYADPTPTPNPNPIHQQNVLKKA 281

BLAST of Bhi03G001252 vs. NCBI nr
Match: XP_038881231.1 (protein SODIUM POTASSIUM ROOT DEFECTIVE 3-like isoform X2 [Benincasa hispida])

HSP 1 Score: 540.8 bits (1392), Expect = 6.9e-150
Identity = 275/281 (97.86%), Postives = 275/281 (97.86%), Query Frame = 0

Query: 1   MKTIDFFCASQASTAVDQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPCSS 60
           MKTIDFFCASQASTAVDQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPCSS
Sbjct: 1   MKTIDFFCASQASTAVDQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPCSS 60

Query: 61  QYSPINPLPYHQLHAAAASPNVAADQIRSGASATHKGLKMKKKKKNSSIITTDFVRWSCA 120
           QYSPINPLPYHQLHAAAASPNVAADQIRSGASATHKGLKMKKKKKNSSIITTDFVRWSCA
Sbjct: 61  QYSPINPLPYHQLHAAAASPNVAADQIRSGASATHKGLKMKKKKKNSSIITTDFVRWSCA 120

Query: 121 KPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSIQLNKKVNSPNEIPQLSKPTAEISLEDD 180
           KPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSIQLNKKVNSPNEIPQLSKPTAEISLEDD
Sbjct: 121 KPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSIQLNKKVNSPNEIPQLSKPTAEISLEDD 180

Query: 181 CKKSPPSNQVISHSQVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKVTIMGNI 240
           CKKSPPSN      QVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKVTIMGNI
Sbjct: 181 CKKSPPSN------QVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKVTIMGNI 240

Query: 241 TPEGMLESVSKVKNAQFWPYADPTPTPNPNPIHQQNVLKKA 282
           TPEGMLESVSKVKNAQFWPYADPTPTPNPNPIHQQNVLKKA
Sbjct: 241 TPEGMLESVSKVKNAQFWPYADPTPTPNPNPIHQQNVLKKA 275

BLAST of Bhi03G001252 vs. NCBI nr
Match: XP_038881232.1 (protein SODIUM POTASSIUM ROOT DEFECTIVE 3-like isoform X3 [Benincasa hispida])

HSP 1 Score: 530.0 bits (1364), Expect = 1.2e-146
Identity = 271/281 (96.44%), Postives = 271/281 (96.44%), Query Frame = 0

Query: 1   MKTIDFFCASQASTAVDQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPCSS 60
           MKTIDFFCASQASTAVDQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPCSS
Sbjct: 1   MKTIDFFCASQASTAVDQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPCSS 60

Query: 61  QYSPINPLPYHQLHAAAASPNVAADQIRSGASATHKGLKMKKKKKNSSIITTDFVRWSCA 120
           QYSPINPLPYHQLHAAAASPNVAADQIRSGASATHKGLKMKKKKKNSSIITTDFVRWSCA
Sbjct: 61  QYSPINPLPYHQLHAAAASPNVAADQIRSGASATHKGLKMKKKKKNSSIITTDFVRWSCA 120

Query: 121 KPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSIQLNKKVNSPNEIPQLSKPTAEISLEDD 180
           KPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSIQLNKKVNSPNEIPQLSKPTAEISLEDD
Sbjct: 121 KPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSIQLNKKVNSPNEIPQLSKPTAEISLEDD 180

Query: 181 CKKSPPSNQVISHSQVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKVTIMGNI 240
           CKKSPPSNQVISHSQVVVLRVSLHCRGCEGKLRKHLSKME          AKKVTIMGNI
Sbjct: 181 CKKSPPSNQVISHSQVVVLRVSLHCRGCEGKLRKHLSKME----------AKKVTIMGNI 240

Query: 241 TPEGMLESVSKVKNAQFWPYADPTPTPNPNPIHQQNVLKKA 282
           TPEGMLESVSKVKNAQFWPYADPTPTPNPNPIHQQNVLKKA
Sbjct: 241 TPEGMLESVSKVKNAQFWPYADPTPTPNPNPIHQQNVLKKA 271

BLAST of Bhi03G001252 vs. NCBI nr
Match: XP_038881233.1 (protein SODIUM POTASSIUM ROOT DEFECTIVE 3-like isoform X4 [Benincasa hispida])

HSP 1 Score: 511.9 bits (1317), Expect = 3.5e-141
Identity = 265/281 (94.31%), Postives = 265/281 (94.31%), Query Frame = 0

Query: 1   MKTIDFFCASQASTAVDQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPCSS 60
           MKTIDFFCASQASTAVDQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPCSS
Sbjct: 1   MKTIDFFCASQASTAVDQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPCSS 60

Query: 61  QYSPINPLPYHQLHAAAASPNVAADQIRSGASATHKGLKMKKKKKNSSIITTDFVRWSCA 120
           QYSPINPLPYHQLHAAAASPNVAADQIRSGASATHKGLKMKKKKKNSSIITTDFVRWSCA
Sbjct: 61  QYSPINPLPYHQLHAAAASPNVAADQIRSGASATHKGLKMKKKKKNSSIITTDFVRWSCA 120

Query: 121 KPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSIQLNKKVNSPNEIPQLSKPTAEISLEDD 180
           KPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSIQLNKKVNSPNEIPQLSKPTAEISLEDD
Sbjct: 121 KPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSIQLNKKVNSPNEIPQLSKPTAEISLEDD 180

Query: 181 CKKSPPSNQVISHSQVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKVTIMGNI 240
           CKKSPPSN      QVVVLRVSLHCRGCEGKLRKHLSKME          AKKVTIMGNI
Sbjct: 181 CKKSPPSN------QVVVLRVSLHCRGCEGKLRKHLSKME----------AKKVTIMGNI 240

Query: 241 TPEGMLESVSKVKNAQFWPYADPTPTPNPNPIHQQNVLKKA 282
           TPEGMLESVSKVKNAQFWPYADPTPTPNPNPIHQQNVLKKA
Sbjct: 241 TPEGMLESVSKVKNAQFWPYADPTPTPNPNPIHQQNVLKKA 265

BLAST of Bhi03G001252 vs. NCBI nr
Match: XP_011653614.1 (protein SODIUM POTASSIUM ROOT DEFECTIVE 3 isoform X1 [Cucumis sativus] >KGN54216.1 hypothetical protein Csa_017964 [Cucumis sativus])

HSP 1 Score: 428.3 bits (1100), Expect = 5.0e-116
Identity = 236/290 (81.38%), Postives = 243/290 (83.79%), Query Frame = 0

Query: 1   MKTIDFFCASQASTAVDQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTT-FPNPPCS 60
           MKTIDFFCASQASTAVDQPSSSPA    GR IDRHNPIIAD RRSN TSRTT FPNPPCS
Sbjct: 1   MKTIDFFCASQASTAVDQPSSSPA----GRFIDRHNPIIADARRSNVTSRTTNFPNPPCS 60

Query: 61  SQYSPINPLPYHQLH--AAAASPNVAADQIR-SGASATHKGLKM-KKKKKNSSIITTDFV 120
           SQYSPINPLPYHQLH  AAAASPNVA DQIR SG S  HK LKM KKKKK+SSIITTDFV
Sbjct: 61  SQYSPINPLPYHQLHAAAAAASPNVAGDQIRPSGVSGNHKDLKMKKKKKKSSSIITTDFV 120

Query: 121 RWSCAKPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSIQLNKKVNSPN-EIPQLSKPTAE 180
           RWSCAKPSDLATPPGSMRYLLNDKSV DGSMDR+ T I +NK   S N + P  SKPT +
Sbjct: 121 RWSCAKPSDLATPPGSMRYLLNDKSVPDGSMDRIPTPIPINKNQPSSNPQDPHHSKPTPQ 180

Query: 181 ISLEDDCKKSPPSNQVISHSQVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKV 240
           IS +DD  KSPPSN      QVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKV
Sbjct: 181 ISSQDDSNKSPPSN------QVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKV 240

Query: 241 TIMGNITPEGMLESVSKVKNAQFWPYADPTPTPNPNP----IHQQNVLKK 281
           TIMGNITP+GMLESVSKVKNAQFWPYADPTPTP PNP     H  NVLKK
Sbjct: 241 TIMGNITPQGMLESVSKVKNAQFWPYADPTPTPTPNPNLNQNHHPNVLKK 280

BLAST of Bhi03G001252 vs. ExPASy TrEMBL
Match: A0A0A0L239 (HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G293260 PE=4 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 2.4e-116
Identity = 236/290 (81.38%), Postives = 243/290 (83.79%), Query Frame = 0

Query: 1   MKTIDFFCASQASTAVDQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTT-FPNPPCS 60
           MKTIDFFCASQASTAVDQPSSSPA    GR IDRHNPIIAD RRSN TSRTT FPNPPCS
Sbjct: 1   MKTIDFFCASQASTAVDQPSSSPA----GRFIDRHNPIIADARRSNVTSRTTNFPNPPCS 60

Query: 61  SQYSPINPLPYHQLH--AAAASPNVAADQIR-SGASATHKGLKM-KKKKKNSSIITTDFV 120
           SQYSPINPLPYHQLH  AAAASPNVA DQIR SG S  HK LKM KKKKK+SSIITTDFV
Sbjct: 61  SQYSPINPLPYHQLHAAAAAASPNVAGDQIRPSGVSGNHKDLKMKKKKKKSSSIITTDFV 120

Query: 121 RWSCAKPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSIQLNKKVNSPN-EIPQLSKPTAE 180
           RWSCAKPSDLATPPGSMRYLLNDKSV DGSMDR+ T I +NK   S N + P  SKPT +
Sbjct: 121 RWSCAKPSDLATPPGSMRYLLNDKSVPDGSMDRIPTPIPINKNQPSSNPQDPHHSKPTPQ 180

Query: 181 ISLEDDCKKSPPSNQVISHSQVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKV 240
           IS +DD  KSPPSN      QVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKV
Sbjct: 181 ISSQDDSNKSPPSN------QVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKV 240

Query: 241 TIMGNITPEGMLESVSKVKNAQFWPYADPTPTPNPNP----IHQQNVLKK 281
           TIMGNITP+GMLESVSKVKNAQFWPYADPTPTP PNP     H  NVLKK
Sbjct: 241 TIMGNITPQGMLESVSKVKNAQFWPYADPTPTPTPNPNLNQNHHPNVLKK 280

BLAST of Bhi03G001252 vs. ExPASy TrEMBL
Match: A0A5A7TZF0 (HMA domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold908G001570 PE=4 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 6.0e-115
Identity = 227/286 (79.37%), Postives = 241/286 (84.27%), Query Frame = 0

Query: 1   MKTIDFFCASQASTAVDQPSSSPA-AAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPCS 60
           MKTIDFFCASQASTAVDQPSS+PA  AA+GR IDRHNPIIADGRRSN TSRT FPNPPCS
Sbjct: 1   MKTIDFFCASQASTAVDQPSSTPATVAAAGRFIDRHNPIIADGRRSNVTSRTNFPNPPCS 60

Query: 61  SQYSPINPLPYHQLHAAAASPNVAADQIRSGASATHKGLKMKKK----KKNSSIITTDFV 120
           SQYSPINPLPYHQLH AAASPNVA DQIR   S  HK LKMKKK    KK+SSI+TTDFV
Sbjct: 61  SQYSPINPLPYHQLH-AAASPNVAGDQIR---SENHKDLKMKKKKKKIKKSSSIVTTDFV 120

Query: 121 RWSCAKPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSIQLNKKVN-SPNEIPQLSKPTAE 180
           RWSCAKPSDLATPPGSMRYLLNDKSVRDGSMDR+ST I ++K +  S  + P   +PT +
Sbjct: 121 RWSCAKPSDLATPPGSMRYLLNDKSVRDGSMDRISTPIPIHKNLTPSDQQHPHQPQPTPQ 180

Query: 181 ISLEDDCKKSPPSNQVISHSQVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKV 240
           IS +D C KSPPSN      QVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKV
Sbjct: 181 ISSDDHCNKSPPSN------QVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKV 240

Query: 241 TIMGNITPEGMLESVSKVKNAQFWPYADPTPTPNPNPIHQQNVLKK 281
           TIMGNITP+GMLESVSKVKNAQFWPYADPTP PN N  H  N L+K
Sbjct: 241 TIMGNITPQGMLESVSKVKNAQFWPYADPTPNPNLNRNHHPNGLEK 276

BLAST of Bhi03G001252 vs. ExPASy TrEMBL
Match: A0A1S3BIC7 (uncharacterized protein LOC103489885 OS=Cucumis melo OX=3656 GN=LOC103489885 PE=4 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 6.0e-115
Identity = 227/286 (79.37%), Postives = 241/286 (84.27%), Query Frame = 0

Query: 1   MKTIDFFCASQASTAVDQPSSSPA-AAASGRAIDRHNPIIADGRRSNFTSRTTFPNPPCS 60
           MKTIDFFCASQASTAVDQPSS+PA  AA+GR IDRHNPIIADGRRSN TSRT FPNPPCS
Sbjct: 1   MKTIDFFCASQASTAVDQPSSTPATVAAAGRFIDRHNPIIADGRRSNVTSRTNFPNPPCS 60

Query: 61  SQYSPINPLPYHQLHAAAASPNVAADQIRSGASATHKGLKMKKK----KKNSSIITTDFV 120
           SQYSPINPLPYHQLH AAASPNVA DQIR   S  HK LKMKKK    KK+SSI+TTDFV
Sbjct: 61  SQYSPINPLPYHQLH-AAASPNVAGDQIR---SENHKDLKMKKKKKKIKKSSSIVTTDFV 120

Query: 121 RWSCAKPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSIQLNKKVN-SPNEIPQLSKPTAE 180
           RWSCAKPSDLATPPGSMRYLLNDKSVRDGSMDR+ST I ++K +  S  + P   +PT +
Sbjct: 121 RWSCAKPSDLATPPGSMRYLLNDKSVRDGSMDRISTPIPIHKNLTPSDQQHPHQPQPTPQ 180

Query: 181 ISLEDDCKKSPPSNQVISHSQVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKV 240
           IS +D C KSPPSN      QVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKV
Sbjct: 181 ISSDDHCNKSPPSN------QVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAKKV 240

Query: 241 TIMGNITPEGMLESVSKVKNAQFWPYADPTPTPNPNPIHQQNVLKK 281
           TIMGNITP+GMLESVSKVKNAQFWPYADPTP PN N  H  N L+K
Sbjct: 241 TIMGNITPQGMLESVSKVKNAQFWPYADPTPNPNLNRNHHPNGLEK 276

BLAST of Bhi03G001252 vs. ExPASy TrEMBL
Match: A0A6J1GEB0 (protein SODIUM POTASSIUM ROOT DEFECTIVE 3-like OS=Cucurbita moschata OX=3662 GN=LOC111453393 PE=4 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 7.1e-108
Identity = 226/285 (79.30%), Postives = 234/285 (82.11%), Query Frame = 0

Query: 1   MKTIDFFCASQASTAV-----DQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPN 60
           MKTI+  CASQASTAV     +QPSS+PA    GRAIDRHNPIIADGRRS  TSR TFPN
Sbjct: 1   MKTINLLCASQASTAVSLPTAEQPSSTPAV---GRAIDRHNPIIADGRRSVTTSR-TFPN 60

Query: 61  PPCSSQYSPINPLPYHQLHAAAASPNVAADQIRSGASATHKGLKMKKKKKNSSIITTDFV 120
           PPCSSQYSPINPLPYHQLH AAA    AADQIRSG SATHKGLK KKKKK SSII+TDFV
Sbjct: 61  PPCSSQYSPINPLPYHQLHPAAA----AADQIRSGGSATHKGLK-KKKKKGSSIISTDFV 120

Query: 121 RWSCAKPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSI---QLNKKVNSPNEIPQLSKPT 180
           RWSCAKPSDLATPPGSMRYLLNDKSV DGS DRLSTSI   Q N K +S  E    SKPT
Sbjct: 121 RWSCAKPSDLATPPGSMRYLLNDKSVPDGSTDRLSTSILSSQTNNKPDSGTENRDESKPT 180

Query: 181 AEISLEDDCKKSPPSNQVISHSQVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAK 240
           AEI LEDDCK SPP N       VVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAK
Sbjct: 181 AEILLEDDCKSSPPFN------HVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDFAAK 240

Query: 241 KVTIMGNITPEGMLESVSKVKNAQFWPYADPTPTPNPNPIHQQNV 278
           KVTIMGNITPEG+LESVSKVKNAQFWP+ADP   P+PNP  QQNV
Sbjct: 241 KVTIMGNITPEGVLESVSKVKNAQFWPFADPISKPDPNP--QQNV 268

BLAST of Bhi03G001252 vs. ExPASy TrEMBL
Match: A0A6J1ILS7 (protein SODIUM POTASSIUM ROOT DEFECTIVE 3-like OS=Cucurbita maxima OX=3661 GN=LOC111477619 PE=4 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 4.6e-107
Identity = 224/288 (77.78%), Postives = 235/288 (81.60%), Query Frame = 0

Query: 1   MKTIDFFCASQASTAV-----DQPSSSPAAAASGRAIDRHNPIIADGRRSNFTSRTTFPN 60
           MKTI+  CASQASTAV     +QPSS+PA    GRAIDRHNPIIADGRRS  TSR TFPN
Sbjct: 1   MKTINLLCASQASTAVSLPTAEQPSSTPAV---GRAIDRHNPIIADGRRSVTTSR-TFPN 60

Query: 61  PPCSSQYSPINPLPYHQLH--AAAASPNVAADQIRSGASATHKGLKMKKKKK-NSSIITT 120
           PPCSSQYSPINPLPYHQLH  AAAA+PN+ ADQI SG SATHKGLK KKKKK  SSII+T
Sbjct: 61  PPCSSQYSPINPLPYHQLHPAAAAATPNLTADQIHSGDSATHKGLKKKKKKKMGSSIIST 120

Query: 121 DFVRWSCAKPSDLATPPGSMRYLLNDKSVRDGSMDRLSTSI---QLNKKVNSPNEIPQLS 180
           DFVRWSC KP DLATP GSMRYLLNDKSVRDGS DRLSTSI   Q N K +S  E    S
Sbjct: 121 DFVRWSCVKPRDLATPLGSMRYLLNDKSVRDGSTDRLSTSILSSQTNNKPDSGTENRDES 180

Query: 181 KPTAEISLEDDCKKSPPSNQVISHSQVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDF 240
           KPTAEI LEDDCK SPPSN       VVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDF
Sbjct: 181 KPTAEILLEDDCKSSPPSN------HVVVLRVSLHCRGCEGKLRKHLSKMEGVNSFNIDF 240

Query: 241 AAKKVTIMGNITPEGMLESVSKVKNAQFWPYADPTPTPNPNPIHQQNV 278
           AAKKVTIMGNITPEG+LESVSKVKNAQFWP+ADP   P+ NP  QQN+
Sbjct: 241 AAKKVTIMGNITPEGVLESVSKVKNAQFWPFADPISKPDLNP--QQNI 276

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G53530.11.8e-3442.39Chloroplast-targeted copper chaperone protein [more]
AT3G53530.24.3e-3342.24Chloroplast-targeted copper chaperone protein [more]
AT2G37390.14.8e-3239.01Chloroplast-targeted copper chaperone protein [more]
AT2G37390.26.3e-3239.01Chloroplast-targeted copper chaperone protein [more]
AT5G02600.21.1e-3138.21Heavy metal transport/detoxification superfamily protein [more]
Match NameE-valueIdentityDescription
Q8RXH82.5e-3342.39Protein SODIUM POTASSIUM ROOT DEFECTIVE 3 OS=Arabidopsis thaliana OX=3702 GN=NAK... [more]
Q58FZ06.8e-3139.01Protein SODIUM POTASSIUM ROOT DEFECTIVE 2 OS=Arabidopsis thaliana OX=3702 GN=NAK... [more]
Q8LDS41.5e-3038.21Protein SODIUM POTASSIUM ROOT DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=NAK... [more]
Q94BT94.1e-1248.48Copper transport protein ATX1 OS=Arabidopsis thaliana OX=3702 GN=ATX1 PE=1 SV=2[more]
O820895.4e-1243.75Copper transport protein CCH OS=Arabidopsis thaliana OX=3702 GN=CCH PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_038881230.13.2e-155100.00protein SODIUM POTASSIUM ROOT DEFECTIVE 3-like isoform X1 [Benincasa hispida][more]
XP_038881231.16.9e-15097.86protein SODIUM POTASSIUM ROOT DEFECTIVE 3-like isoform X2 [Benincasa hispida][more]
XP_038881232.11.2e-14696.44protein SODIUM POTASSIUM ROOT DEFECTIVE 3-like isoform X3 [Benincasa hispida][more]
XP_038881233.13.5e-14194.31protein SODIUM POTASSIUM ROOT DEFECTIVE 3-like isoform X4 [Benincasa hispida][more]
XP_011653614.15.0e-11681.38protein SODIUM POTASSIUM ROOT DEFECTIVE 3 isoform X1 [Cucumis sativus] >KGN54216... [more]
Match NameE-valueIdentityDescription
A0A0A0L2392.4e-11681.38HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G293260 PE=4 SV... [more]
A0A5A7TZF06.0e-11579.37HMA domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A1S3BIC76.0e-11579.37uncharacterized protein LOC103489885 OS=Cucumis melo OX=3656 GN=LOC103489885 PE=... [more]
A0A6J1GEB07.1e-10879.30protein SODIUM POTASSIUM ROOT DEFECTIVE 3-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1ILS74.6e-10777.78protein SODIUM POTASSIUM ROOT DEFECTIVE 3-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.70.100coord: 191..256
e-value: 3.2E-15
score: 58.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 16..35
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 261..281
NoneNo IPR availablePANTHERPTHR46119:SF15PROTEIN SODIUM POTASSIUM ROOT DEFECTIVE 2coord: 1..271
IPR006121Heavy metal-associated domain, HMAPFAMPF00403HMAcoord: 199..251
e-value: 5.7E-12
score: 45.9
IPR006121Heavy metal-associated domain, HMAPROSITEPS50846HMA_2coord: 195..252
score: 10.840837
IPR006121Heavy metal-associated domain, HMACDDcd00371HMAcoord: 198..251
e-value: 1.76212E-11
score: 56.4601
IPR044526Protein SODIUM POTASSIUM ROOT DEFECTIVE 1/2/3PANTHERPTHR46119OS08G0405700 PROTEINcoord: 1..271
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 194..252

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M001252Bhi03M001252mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding